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RNA Polymerase I (0):
Recent article
RNA Polymerase I
Molecular Cell (39)4 2010
Abstract
We show that the Pol I-specific subunits A49 and A34.5 form a subcomplex that binds DNA and is related to TFIIF and TFIIE. The N-terminal regions of A49 and A34.5 form a dimerization module that stimulates polymerase-intrinsic RNA cleavage and has a fold that resembles the TFIIF core. The C-terminal...
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PMID: 20797630
PDF is available here.
PDF is available here.
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Abstract
I (RPB1) and nuclear ribosomal large subunit (LSU) rDNA gene sequences. Based on the result of this initial screen, 62 collections representing the full range of genetic diversity sampled were subjected to multigene phylogenetic species recognition based on genealogical concordance (GCPSR). The 62-t...
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PMID: 20580850
PDF is available here.
PDF is available here.
Abstract
We show that after infection of human cells with herpes simplex virus type 1 (HSV-1) the rate of ribosome biogenesis is modulated independently of RNA Pol I activity by a dramatic change in the rRNA maturation pathway. This process permits control of the ribosome biogenesis rate, giving the possibil...
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PMID: 19934231
PDF is available here.
PDF is available here.
Abstract
We report the recovery of infectious FMDV from cDNAs based on an efficient in vivo RNA polymerase I (polI) transcription system. Intracellular transcription of the full-length viral genome from polI-based vectors resulted in efficient formation of infectious virus displaying a genetic marker. Compar...
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PMID: 20011974
PDF is available here.
PDF is available here.
Abstract
We used a differential chromatin immunoprecipitation procedure coupled to whole-genome microarray detection in Saccharomyces cerevisiae. We find that TATA-binding protein (TBP) turnover is low at RNA polymerase I (Pol I) promoters. Whereas RNA polymerase III (Pol III) promoters represent an intermed...
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PMID: 19767748
PDF is available here.
PDF is available here.
Abstract
We have raised antibodies against the E. histolytica homologue of fibrillarin, a highly conserved protein known to be a marker for nucleolus. These antibodies cross-reacted preferentially with the nuclear periphery, forming a peripheral ring. There was complete colocalization of fibrillarin with the...
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PMID: 19416742
PDF is available here.
PDF is available here.
Journal of Rheumatology (36)7 2009
Abstract
Forty-six cases (38 female; 40 diffuse cutaneous SSc) were identified. Mean age at SSc and SRC onset was 52.8 years +/- 13.2 and 55.4 years +/- 11.8, respectively. ANA were present in 44 patients (96%). Anti-topo I antibodies were detected in 30 (65%), anti-RNAP I-III in 7 (15%). No differences emer...
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PMID: 19487262
PDF is available here.
PDF is available here.
EMBO Journal (28)7 2009
Abstract
I can transcribe through nucleosome templates and that this requires structural rearrangement of the nucleosomal core particle. The subunits of the histone chaperone FACT (facilitates chromatin transcription), SSRP1 and Spt16, co-purify and co-immunoprecipitate with mammalian Pol I complexes. In cel...
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PMID: 19214185
PDF is available here.
PDF is available here.
EMBO Reports (9)12 2008
Abstract
Abstract
We also described the development of a reverse genetics system for HTNV strain 84FLi. The RNA polymerase I (pol I)-mediated transcription system was used to generate artificial viral RNA genome segments (minigenomes), which contained the chloramphenicol acetyltransferase (CAT) reporter gene in antis...
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PMID: 19133012
PDF is available here.
PDF is available here.
Abstract
I (Pol I). Cells lacking the Uaf30 subunit of UAF reduce the rRNA synthesis rate by approximately 70% compared to wild-type cells and produce rRNA using both Pol I and Pol II. Miller chromatin spreads demonstrated that even though there is an overall reduction in rRNA synthesis in uaf30 mutants, the...
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PMID: 18765638
PDF is available here.
PDF is available here.
Journal of Molecular Biology (382)2 2008
Abstract
We have analyzed at which step of the transcription cycle CSB influences in vitro transcription by RNA Pol I. We demonstrate that CSB stimulates elongation of RNA Pol I in an ATP-independent manner. Moreover, CSB can be cross-linked to the rDNA promoter and gene-internal sequences. Partial deletion...
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PMID: 18656484
PDF is available here.
PDF is available here.
Abstract
We describe a method of reverse genetics to recover infectious RVFV from transfected plasmids based on the use of the cellular RNA polymerase I promoter to synthesize viral transcripts. We compared its efficiency with a system using T7 RNA polymerase and found that both are equally efficient for the...
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PMID: 18614193
PDF is available here.
PDF is available here.
Abstract
We show that the glycogen synthase kinase (GSK) 3beta, which phosphorylates the tumour suppressor PTEN (phosphatase and tensin homologue deleted on chromosome 10), is selectively enriched in nucleoli of RAS-transformed cells. Immunoprecipitation and chromatin immunoprecipitation assays performed on...
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PMID: 18490923
PDF is available here.
PDF is available here.
Journal of General Virology (89)Pt 9 2008
Abstract
We report the establishment and characterization of two different systems to rescue the RVFV wild-type strain ZH548. The first system is based on the BHK-21 cell clone BSR-T7/5, which stably expresses T7 RNA polymerase (T7 pol). Rescue of wild-type RVFV was achieved with three T7 pol-driven cDNA pla...
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PMID: 18753225
PDF is available here.
PDF is available here.
Nucleic Acids Research (36)15 2008
Abstract
We showed that phosphorylation of UBF by the MAP-kinase ERK regulates chromatin folding and transcription elongation, explaining the rapid response of the ribosomal RNA genes to growth factors. Here we have investigated the roles the UBF variants play in the response of these genes to ERK activity....
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PMID: 18676449
PDF is available here.
PDF is available here.
Abstract
We show in yeast, that the presence of yeast linker histone Hho1p represses expression of a pol II transcribed gene (MET15) embedded in the rDNA. In vivo deletions of Hho1p sequences showed that the second globular domain is sufficient for that repression, whereas the presence of the N terminus is r...
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PMID: 18687885
PDF is available here.
PDF is available here.
Abstract
We show that CK2 phosphorylates the transcription initiation factor TIF-IA at serines 170 and 172 (Ser170/172), and this phosphorylation triggers the release of TIF-IA from Pol I after transcription initiation. Inhibition of Ser170/172 phosphorylation or covalent tethering of TIF-IA to the RPA43 sub...
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PMID: 18559419
PDF is available here.
PDF is available here.
Abstract
I (RPI) transcription in nucleolar development during major transcriptional activation (MTA) in cattle. Late eight-cell embryos were cultured in the absence (control group) or presence of actinomycin D (AD) (RPI inhibition, AD 0.2 microg/ml; total transcriptional inhibition, AD 2.0 microg/ml). Late...
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PMID: 18196555
PDF is available here.
PDF is available here.
Molecular Cell (30)5 2008
Abstract
In a recent issue of Molecular Cell, Gorski et al. (2008) demonstrate directly that polymerase assembly kinetics regulate Pol I transcription.
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PMID: 18538652
PDF is available here.
PDF is available here.
Molecular Cell (30)4 2008
Abstract
We have used single-cell fluorescence imaging combined with computational modeling and chromatin immunoprecipitation to analyze transcription complex dynamics in gene regulation during the cell cycle in living cells. We demonstrate a link between the dynamics of RNA polymerase I (RNA Pol I) assembly...
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PMID: 18498750
PDF is available here.
PDF is available here.
Journal of Cell Biology (181)4 2008
Abstract
We examine here whether transcription occurs in discrete sites (factories) containing the required machinery and whether these sites specialize in transcribing different genes. We cotransfected plasmids encoding a common origin of replication but different transcription units into cells, where they...
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PMID: 18490511
PDF is available here.
PDF is available here.
Abstract
The swine influenza virus (SIV) strain A/Swine/TianJin/01/2004(H1N1) (A/S/TJ/04) was rescued successfully by an eight-plasmid rescue system. The cDNAs of SIV 8 gene segments were synthesized by RT-PCR and cloned into the RNA polymerase I/II bidirection expression vector PHW2000 independently, result...
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PMID: 18724708
PDF is available here.
PDF is available here.
Genes & Development (22)8 2008
Abstract
We report that the activity of Rat1 is also required for efficient termination by RNA polymerase I (Pol I) on the rDNA. In strains lacking catalytically active Rat1 or its cofactor Rai1, Pol I reads through the major, "Reb1-dependent" terminator (T1) but stops downstream at the "fail-safe" terminato...
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PMID: 18413717
PDF is available here.
PDF is available here.
Genes & Development (22)8 2008
Abstract
We demonstrate that both Rat1p exonuclease and Sen1p helicase are required for efficient termination by promoting degradation of the nascent transcript associated with Pol II, following mRNA 3' end processing. Similarly, Pol I termination relies on prior Rnt1p cleavage at the 3' end of the pre-rRNA...
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PMID: 18413718
PDF is available here.
PDF is available here.
Journal of Cell Science (121)Pt 7 2008
Abstract
We have used RNA interference to deplete key structural nuclear proteins, the nuclear lamins. In HeLa cells, we show that reduced expression of lamin B1, but not lamin A/C, severely inhibits RNA synthesis--first by RNA polymerase II and later by RNA polymerase I. Declining levels of transcription co...
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PMID: 18334554
PDF is available here.
PDF is available here.
Journal of Cell Biology (180)6 2008
Abstract
I transcription has implicated polymeric actin, consorting with an isoform of its classical partner myosin. Both studies are critically discussed here, and although there are several issues to be resolved, it now seems reasonable to start thinking about functions for both monomeric and assembled act...
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PMID: 18347069
PDF is available here.
PDF is available here.
Abstract
We investigated a novel vector system for integration and expression of a transgene. In this system, the transgene expression was driven by an endogenous RNA polymerase I (Pol I) promoter after being integrated into the ribosomal DNA (rDNA) locus. Human coagulation factor IX coding sequence (FIX), w...
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PMID: 18194663
PDF is available here.
PDF is available here.
Abstract
Rpa34 and Rpa49 are nonessential subunits of RNA polymerase I, conserved in species from Saccharomyces cerevisiae and Schizosaccharomyces pombe to humans. Rpa34 bound an N-terminal region of Rpa49 in a two-hybrid assay and was lost from RNA polymerase in an rpa49 mutant lacking this Rpa34-binding do...
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PMID: 18086878
PDF is available here.
PDF is available here.
Molecular Microbiology (67)4 2008
Abstract
We show that S. cerevisiae histone H1 represses recombination at the rDNA without affecting Pol II gene silencing. The most highly studied repressor of recombination at the rDNA is the Silent information regulator protein Sir2. We find that cells lacking histone H1 do not exhibit a premature-ageing...
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PMID: 18179596
PDF is available here.
PDF is available here.
Journal of Virology (82)3 2008
Abstract
We established an alternative reverse genetics system for influenza virus using the canine polymerase I (PolI) promoter sequence that works efficiently in the Madin-Darby canine kidney cell line, a cell line approved for human vaccine production. Using this system, we were able to generate H5N1 vacc...
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PMID: 18045936
PDF is available here.
PDF is available here.
Genes & Development (22)3 2008
Abstract
We show that actin and NM1 act in concert to promote RNA polymerase I (Pol I) transcription. Drugs that prevent actin polymerization or inhibit myosin function inhibit Pol I transcription in vivo and in vitro. Mutants that stabilize the polymeric state actin are tightly associated with Pol I and act...
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PMID: 18230700
PDF is available here.
PDF is available here.
Nucleic Acids Research (36)3 2008
Abstract
We used a rapid and efficient way to purify yeast RNA Pol I to determine 13 phosphoserines and -threonines. Seven of these phosphoresidues could be located in the 3D-homology model for Pol I, five of them are more at the surface. The single phosphorylated residues were systematically mutated and the...
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PMID: 18084032
PDF is available here.
PDF is available here.
Early pre-implantation lethality in mice carrying truncated mutation in the RNA polymerase 1-2 gene.
Abstract
We have identified an insertional mutation (Rpo1-2(Gt)) in the 14th exon of Rpo1-2, resulting in a truncation of 312aa from the C-terminal. In Rpo1-2(Gt/Gt) embryos, the synthesis of rRNA was severely impaired. Rpo1-2(Gt/Gt) embryos could develop to the morula stage, and thereafter displayed nucleol...
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PMID: 18023416
PDF is available here.
PDF is available here.
Abstract
By using biochemical and reverse genetic means we show here that human RNase P is required for efficient transcription of rDNA by Pol I. Thus, inactivation of RNase P by targeting its protein subunits for destruction by RNA interference or its H1 RNA moiety for specific cleavage causes marked reduct...
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PMID: 19115013
PDF is available here.
PDF is available here.
Tsitologiia (50)4 2008
Abstract
We describe how the nucleolus reacts to inhibition of protein synthesis as revealed by labeling with a new monoclonal antibody A3. In normal cells A3 antigen is observed as numerous foci within the nucleolus. During mitosis A3 antigen is located in a few foci on chromosomes. Regions of A3 localizati...
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PMID: 18664117
PDF is available here.
PDF is available here.
Tsitologiia (50)8 2008
Abstract
We have examined the features of organization and molecular composition of nucleolus like body, or postnucleolus, in two groups of oocytes with different chromatin configuration from mouse antral follicles. Using light and electron immunocytochemistry, we have defined the dynamics of ribosomal RNA s...
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PMID: 18822787
PDF is available here.
PDF is available here.
Gene Expression (14)3 2008
Abstract
We developed a novel assay similar to chromatin immunoprecipitation assays. We found that RNA polymerase I can be recruited to a template in the absence of active Rrn3. However, that complex will not initiate transcription, even after Rrn3 is added to the reaction. Interestingly, the complex that fo...
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PMID: 18590050
PDF is available here.
PDF is available here.
Abstract
We report the development and optimization of two minigenome rescue systems for Nipah virus, a member of the Paramyxoviridae family. One is mediated by the T7 RNA polymerase supplied either by a constitutively expressing cell line or by transfection of expression plasmids and is thus independent fro...
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PMID: 17904180
PDF is available here.
PDF is available here.
Abstract
We show that Rnt1p physically interacts with RNA polymerase I (RNAPI) and its deletion decreases the transcription of the rRNA gene and increases the number of rRNA genes with an open chromatin structure. In contrast, depletion of ribosomal proteins or factors that impair RNAPI termination did not i...
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PMID: 17991894
PDF is available here.
PDF is available here.
Abstract
We have evaluated heterologous expression systems using vectors with two different polypyrimidine tracts in the splice acceptor site by measuring a reporter gene transcribed from L. tarentolae RNA polymerase I promoter. Our data indicate that the efficiency of chloramphenicol acetyl transferase expr...
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PMID: 18060313
PDF is available here.
PDF is available here.
Abstract
HMO1 is a high-mobility group B protein that plays a role in transcription of genes encoding rRNA and ribosomal proteins (RPGs) in Saccharomyces cerevisiae. This study uses genome-wide chromatin immunoprecipitation to study the roles of HMO1, FHL1, and RAP1 in transcription of these genes as well as...
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PMID: 17646381
PDF is available here.
PDF is available here.
Abstract
Transfection of the RNA polymerase I-HCV IRES plasmid into human hepatoma Huh-7 and HepG2 cells resulted in luciferase gene expression. Deletion of the IIIf domain in HCV IRES dramatically reduced luciferase activity. CONCLUSION: Our results indicated that the plasmid vector system-based on RNA poly...
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PMID: 17632032
PDF is available here.
PDF is available here.
Abstract
We have epitope tagged the specific subunit RPB6z and tandem affinity purified RNA polymerase I from crude extract. The purified enzyme was active in both a nonspecific and a promoter-dependent transcription assay and exhibited enriched protein bands with apparent sizes of 31, 29, and 27 kDa. p31 an...
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PMID: 17606628
PDF is available here.
PDF is available here.
Abstract
We report that the absence of the Slx5-Slx8 complex, a newly identified player in the SUMO (small ubiquitin-like modifier) pathway, led to increased Rad51-dependent and Rad51-independent recombination. The increases were most striking during S phase, suggesting an accumulation of DNA lesions during...
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PMID: 17591698
PDF is available here.
PDF is available here.
Molecular Cell (27)4 2007
Abstract
We show that a complex consisting of CSB, RNA polymerase I (Pol I), and histone methyltransferase G9a is present at active rRNA genes. G9a methylates histone H3 on lysine 9 (H3K9me2) in the pre-rRNA coding region and facilitates the association of heterochromatin protein 1gamma (HP1gamma) with rDNA....
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PMID: 17707230
PDF is available here.
PDF is available here.
Genes & Development (21)16 2007
Abstract
We characterize human t-UTPs and establish that their ability to link transcription and pre-rRNA processing is evolutionarily conserved. The pol I transcription factor UBF binds extensively across rDNA throughout the cell cycle, resulting in a specialized form of chromatin that is the hallmark of ac...
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PMID: 17699751
PDF is available here.
PDF is available here.
Abstract
We propose a role for the methyl-CpG binding domain protein MBD3 in epigenetically maintaining active rRNA promoters. We show that MBD3 is localized to the nucleolus, colocalizes with upstream binding factor, and binds to unmethylated rRNA promoters. Knockdown of MBD3 by small interfering RNA result...
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PMID: 17452452
PDF is available here.
PDF is available here.
Abstract
I promoters, respectively. In avian cells, transcription/replication activities were in the same range with all ribonucleoproteins tested. In human cells, ribonucleoproteins derived from A/Mallard/Marquenterre/MZ237/83 showed reduced transcription/replication activity and reduced NP binding to the P...
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PMID: 17270230
PDF is available here.
PDF is available here.
Abstract
We monitor RNA polymerase I (Pol I) activity in mouse cells exposed to genotoxic stress and show that induction of DNA breaks leads to a transient repression in Pol I transcription. Surprisingly, we find Pol I inhibition is not itself the direct result of DNA damage but is mediated by ATM kinase act...
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PMID: 17554310
PDF is available here.
PDF is available here.
Genes to Cells (12)6 2007
Abstract
We reported the isolation of condensin mutants of Saccharomyces cerevisiae that were defective in carrying long rDNA repeat due to the loss of the replication fork barrier (RFB) protein Fob1p; thus the repeat in the mutants were dramatically contracted. The reintroduction of the FOB1 gene suppressed...
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PMID: 17573776
PDF is available here.
PDF is available here.
Cell Proliferation (40)3 2007
Abstract
Our data presented here argue for a crucial role of pKi-67 in RNA polymerase I-dependent nucleolar rRNA synthesis....
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PMID: 17531085
PDF is available here.
PDF is available here.
Trends in Microbiology (15)6 2007
Abstract
We discuss recent advances in understanding the role of chromosomal nuclear positioning in the regulation of gene expression in T. brucei....
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PMID: 17481901
PDF is available here.
PDF is available here.
Molecular Cell (26)3 2007
Abstract
We show that the human Maf1 protein negatively regulates transcription by all three nuclear Pols. Changes in Maf1 expression affect Pol I- and Pol III-dependent transcription in human glioblastoma lines. These effects are mediated, in part, through the ability of Maf1 to repress transcription of the...
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PMID: 17499043
PDF is available here.
PDF is available here.
Abstract
We then carried out functional analysis of two of these sites, serines 389 and 584. Serine-alanine substitution mutations of 389 (S389A) abrogated rRNA transcription in vitro and in vivo, whereas mutation of serine 584 (S584A) reduced transcription in vivo but not in vitro. In contrast, serine-gluta...
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PMID: 17182730
PDF is available here.
PDF is available here.
Abstract
Molecular Cell (26)2 2007
Abstract
We describe the identification of a point mutation in the second largest subunit of RNA polymerase I near the active center of the enzyme that results in an elongation-defective enzyme in the yeast Saccharomyces cerevisiae. In vivo, this mutant shows significant defects in rRNA processing and riboso...
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PMID: 17466624
PDF is available here.
PDF is available here.
Abstract
I that controls ribosomal RNA synthesis and ribosome biogenesis. The type 1 insulin-like growth factor receptor (IGF-IR) and its docking protein, insulin receptor substrate-1 (IRS-1) control, in a non-redundant way, about 50% of cell and body size. This is certainly true in mice, flies and cells in...
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PMID: 17404503
PDF is available here.
PDF is available here.
Journal of General Virology (88)Pt 4 2007
Abstract
The currently available reverse-genetics systems for Influenza A virus are all based on transcription of genomic RNA by RNA polymerase I, but the species specificity of this polymerase is a disadvantage. A reverse-genetics vector containing a T7 RNA polymerase promoter, hepatitis delta virus ribozym...
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PMID: 17374773
PDF is available here.
PDF is available here.
Abstract
We used tandem affinity purification (TAP) to identify subunits of RNAP II and III. Mass spectrometric analysis of the complexes co-purified with TAP-tagged LmRPB2 (encoded by LmjF31.0160) identified seven RNAP II subunits: RPB1, RPB2, RPB3, RPB5, RPB7, RPB10 and RPB11. With the exception of RPB10 a...
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PMID: 17275824
PDF is available here.
PDF is available here.