Find your PDFs fast.
Welcome to the Pubget Medical Subject Headings (MESH) browser. Click on a topic or subtopic below to explore related papers. In the gold box is the most recent paper about the current MESH term, and below are 20 related papers.
RNA Polymerase III (0):
Recent article
RNA Polymerase III
Abstract
We find that human MAF1 is absolutely required for pol III repression in response to serum starvation or TORC1 inhibition by rapamycin or Torin1. The protein is phosphorylated mainly on residues S60, S68, and S75, and this inhibits its pol III repression function. The responsible kinase is mTORC1, w...
|
PMID: 20516213
PDF is available here.
PDF is available here.
| < More recent | Older article > |
Clinica Chimica Acta (411)9-10 2010
Abstract
These new ELISA methods for AFA, anti-RNAP III and anti-PM-Scl detection have good diagnostic specificity, and may help identify a subset of SSc patients ACA and anti-topo I-negative.
Copyright 2010 Elsevier B.V. All rights reserved....
|
PMID: 20138166
PDF is available here.
PDF is available here.
Abstract
Streptomyces lydicus NRRL2433 and S. spectabilis NRRL2494 produce two inhibitors of bacterial RNA polymerase: the 3-acyltetramic acid streptolydigin and the naphthalenic ansamycin streptovaricin, respectively. Both strains are highly resistant to their own antibiotics. Independent expression of the...
|
PMID: 20176899
PDF is available here.
PDF is available here.
Abstract
We extend this finding by demonstrating that four of the five RNAPIII-specific subunits are also homologous to transcription factors of RNAPII. We use the available evidence to propose an evolutionary history of the eukaryotic RNAPs and argue that the increases in the number of subunits that occurre...
|
PMID: 20026480
PDF is available here.
PDF is available here.
Journal of Rheumatology (37)5 2010
Abstract
Journal of Rheumatology (37)3 2010
Abstract
Twenty-eight patients were included in this analysis, including 17 with diffuse cutaneous (dcSSc) and 11 with limited cutaneous SSc (lcSSc). The mean disease duration at diagnosis with GAVE was 21.5 months for dcSSc and 84.3 months for lcSSc (p = 0.025). Seventy-six percent of patients with dcSSc de...
|
PMID: 20080908
PDF is available here.
PDF is available here.
Abstract
We detected maturely processed MuHV-4 miRNAs within total RNA from lytically infected cells in vitro and infected tissues ex vivo, using a highly sensitive reverse ligation meditated RT-PCR strategy. We determined that the MuHV-4 microRNAs are biologically active during infection by a luciferase rep...
|
PMID: 19948768
PDF is available here.
PDF is available here.
Abstract
We show that TLS (translocated in liposarcoma), a protein originally identified as the product of a chromosomal translocation and which associates with both RNAP II and the spliceosome, also represses transcription by RNAP III. TLS was found to repress transcription from all three classes of RNAP II...
|
PMID: 19841068
PDF is available here.
PDF is available here.
Nature Immunology (10)10 2009
Abstract
Abstract
We used a differential chromatin immunoprecipitation procedure coupled to whole-genome microarray detection in Saccharomyces cerevisiae. We find that TATA-binding protein (TBP) turnover is low at RNA polymerase I (Pol I) promoters. Whereas RNA polymerase III (Pol III) promoters represent an intermed...
|
PMID: 19767748
PDF is available here.
PDF is available here.
Nature Immunology (10)10 2009
Abstract
We identify a novel DNA-sensing pathway involving RNA polymerase III and RIG-I. In this pathway, AT-rich double-stranded DNA (dsDNA) served as a template for RNA polymerase III and was transcribed into double-stranded RNA (dsRNA) containing a 5'-triphosphate moiety. Activation of RIG-I by this dsRNA...
|
PMID: 19609254
PDF is available here.
PDF is available here.
Plant Physiology (150)3 2009
Abstract
We focus on the identification of factors controlling the processing of tRNA-snoRNA dicistronic precursors (pre-tsnoRNA) synthesized by RNA polymerase III and producing tRNA(Gly) and C/D snoR43. We produced radiolabeled RNA probes corresponding to different pre-tsnoRNA mutants to test their impact o...
|
PMID: 19420328
PDF is available here.
PDF is available here.
Journal of Rheumatology (36)7 2009
Abstract
Forty-six cases (38 female; 40 diffuse cutaneous SSc) were identified. Mean age at SSc and SRC onset was 52.8 years +/- 13.2 and 55.4 years +/- 11.8, respectively. ANA were present in 44 patients (96%). Anti-topo I antibodies were detected in 30 (65%), anti-RNAP I-III in 7 (15%). No differences emer...
|
PMID: 19487262
PDF is available here.
PDF is available here.
Abstract
I (ATA) and anti-RNA polymerase III (ARA) antibodies. The presence of apoptotic cells in cultures of circulating lymphocytes was investigated using the TUNEL (terminal deoxynucleotidyl transferase-mediated deoxyuridine triphosphate nick end labeling) technique. ACAs were present in 16 (41%), ATA in...
|
PMID: 19050892
PDF is available here.
PDF is available here.
EMBO Journal (28)7 2009
Abstract
I can transcribe through nucleosome templates and that this requires structural rearrangement of the nucleosomal core particle. The subunits of the histone chaperone FACT (facilitates chromatin transcription), SSRP1 and Spt16, co-purify and co-immunoprecipitate with mammalian Pol I complexes. In cel...
|
PMID: 19214185
PDF is available here.
PDF is available here.
Abstract
We designed and used a short hairpin RNA to inhibit c-Myc expression in Colo 320 cells and validated its effect on cell proliferation. In this study, four c-Myc-shRNA expression vectors were constructed and introduced into Colo 320 cells. The effects of c-Myc silencing on tumor cell growth was asses...
|
PMID: 19116866
PDF is available here.
PDF is available here.
Abstract
We describe the use of iodinated secondary antibodies for immunodetection of an autoantigen during HPLC purification....
|
PMID: 19378082
PDF is available here.
PDF is available here.
Abstract
We show that in Saccharomyces cerevisiae tRNA genes (tDNAs) and even partially assembled Pol III complexes containing only the transcription factor TFIIIC can exhibit chromatin boundary functions both as heterochromatin barriers and as insulators to gene activation. Both the TRT2 tDNA and the ETC4 s...
|
PMID: 18849469
PDF is available here.
PDF is available here.
EMBO Reports (9)12 2008
Abstract
Abstract
RNA polymerase (Pol) III is the largest of the RNA polymerases with seventeen subunits. It is responsible for the production of short, untranslated RNAs that play important roles in determining the biosynthetic capacity of the cell. Pol III transcription is generally elevated in transformed cells an...
|
PMID: 18971635
PDF is available here.
PDF is available here.
Biochemistry (Washington) (1779)11 2008
Abstract
A compelling tool for functional genetics is to silence the expression of multiple related genes concomitantly and reversibly. Such a tool will accelerate the understanding on gene interaction in signaling pathway and the development of comprehensive animal models for human diseases. Multiple gene si...
|
PMID: 18439919
PDF is available here.
PDF is available here.
Journal of Biological Chemistry (283)39 2008
Abstract
We present the implementation of a quantitative chromatin immunoprecipitation assay in the RNA polymerase III (pol III) system that allowed us to measure the absolute in vivo occupancy of pol III and its two transcription factors, TFIIIC and TFIIIB, on a subset of pol III genes. The crucial point of...
|
PMID: 18667429
PDF is available here.
PDF is available here.
Nature Genetics (40)9 2008
Abstract
We generate Max loss-of-function and reduction-of-function mutations in Drosophila melanogaster to address the contribution of Max to Myc-dependent growth control. We find that many biological activities of Myc do not, or only partly, require the association with Max--for example, the control of end...
|
PMID: 19165923
PDF is available here.
PDF is available here.
Abstract
We report the polyadenylation of short interspersed elements (SINEs) B2 and VES transcripts generated by RNA polymerase III. HeLa cells were transfected with SINE constructs with or without polyadenylation signals. The analyses of the SINE transcripts showed that only the RNAs with the AAUAAA-signal...
|
PMID: 18658125
PDF is available here.
PDF is available here.
Abstract
We describe a completely defined in vitro system for transcription of a human tRNA gene assembled into a chromatin template. Transcriptional activation and histone acetylation in this system depend on recruitment of p300 by general initiation factor TFIIIC, thus providing a new paradigm for recruitm...
|
PMID: 18644873
PDF is available here.
PDF is available here.
Genes & Development (22)16 2008
Abstract
We analyze condensin binding to budding yeast chromosomes using high-resolution oligonucleotide tiling arrays. Condensin-binding sites coincide with those of the loading factor Scc2/4 of the related cohesin complex. The sites map to tRNA and other genes bound by the RNA polymerase III transcription...
|
PMID: 18708580
PDF is available here.
PDF is available here.
Genes & Development (22)16 2008
Abstract
We find that the chromosome-condensing complex, condensin, is involved in the clustering of tRNA genes. Conditionally defective mutations in all five subunits of condensin, which we confirm is bound to active tRNA genes in the yeast genome, lead to loss of both pol II transcriptional silencing near...
|
PMID: 18708579
PDF is available here.
PDF is available here.
Genes & Development (22)14 2008
Abstract
We performed genome-wide location analysis of this factor. Under normal growth conditions, TFIIS was detected on Pol II-transcribed genes, and TFIIS occupancy was well correlated with that of Pol II, indicating that TFIIS recruitment is not restricted to NTP-depleted cells. Unexpectedly, TFIIS was a...
|
PMID: 18628399
PDF is available here.
PDF is available here.
Journal of Cell Biology (182)1 2008
Abstract
We observe that primary miRNA (pri-miRNA) transcripts retained at transcription sites due to the deletion of 3'-end processing signals are converted more efficiently into precursor miRNAs (pre-miRNAs) than pri-miRNAs that are cleaved, polyadenylated, and released. Flanking exons, which also increase...
|
PMID: 18625843
PDF is available here.
PDF is available here.
Journal of Biological Chemistry (283)28 2008
Abstract
We show that Rat1a fibroblasts undergoing oncogenic transformation by the TATA-binding protein or c-Myc display enhanced RNA pol III transcription. Decreased expression of the RNA pol III-specific transcription factor Brf1 prevented this increase in RNA pol III transcription. Although the overall pr...
|
PMID: 18456653
PDF is available here.
PDF is available here.
Rheumatology (47)7 2008
Abstract
We report the outcome of a study on a large population of SSc patients that shows the ARA ELISA to be of high analytical sensitivity and specificity. We confirm that there is minimal overlap between ARA and other SSc-specific autoantibodies. Additionally, it is demonstrated that the presence of ARA...
|
PMID: 18499715
PDF is available here.
PDF is available here.
Abstract
5S rRNA genes from Saccharomyces cerevisiae were examined by Miller chromatin spreading, representing the first quantitative analysis of RNA polymerase III genes in situ by electron microscopy. These very short genes, approximately 132 nucleotides (nt), were engaged by one to three RNA polymerases....
|
PMID: 18474615
PDF is available here.
PDF is available here.
Journal of Biological Chemistry (283)25 2008
Abstract
We conclude that phosphorylation of Maf1 inside the nucleus acts both directly by decreasing of Maf1-mediated repression of Pol III and indirectly by stimulation of Msn5 binding and export of nuclear Maf1 to the cytoplasm....
|
PMID: 18445601
PDF is available here.
PDF is available here.
B M B Reports (41)5 2008
Abstract
We have developed an effective strategy to express multiple shRNAs (small hairpin RNA) simultaneously using multiple RNA Polymerase III (Pol III) promoters in a single vector. Our data demonstrate that multiple shRNAs expressed from Pol III promoters have a synergistic effect in repressing the targe...
|
PMID: 18510865
PDF is available here.
PDF is available here.
Abstract
PTEN, a tumor suppressor whose function is frequently lost in human cancers, possesses a lipid phosphatase activity that represses phosphatidylinositol 3-kinase (PI3K) signaling, controlling cell growth, proliferation, and survival. The potential for PTEN to regulate the synthesis of RNA polymerase...
|
PMID: 18391023
PDF is available here.
PDF is available here.
Nucleic Acids Research (36)11 2008
Abstract
We combine chromatin immunoprecipitation with RNA interference to demonstrate that the RPC3/6/7 subcomplex is required for pol III recruitment in mammalian cells. Specific knockdown of RPC6 by RNAi results in post-transcriptional depletion of the other components of the subcomplex, RPC3 and RPC7, wi...
|
PMID: 18487626
PDF is available here.
PDF is available here.
Circulation Research (102)10 2008
Abstract
We have identified cyclin (Cyc)D2, a G(1) cyclin implicated in mediating S phase entry, as a potential regulator of hypertrophic growth in adult post mitotic myocardium. To examine the role of CycD2 and its downstream targets, we subjected CycD2-null mice to mechanical stress. Hypertrophic growth in...
|
PMID: 18420946
PDF is available here.
PDF is available here.
Journal of Cell Biology (181)4 2008
Abstract
We examine here whether transcription occurs in discrete sites (factories) containing the required machinery and whether these sites specialize in transcribing different genes. We cotransfected plasmids encoding a common origin of replication but different transcription units into cells, where they...
|
PMID: 18490511
PDF is available here.
PDF is available here.
Abstract
We analyzed both yeast Pol II and Pol III by multiplexed mass spectrometric analysis using various proteases and both collision induced and electron transfer dissociation. The cumulative data obtained from using the various proteases (trypsin, chymotrypsin, Glu-C and Lys-C) and the two peptide fragm...
|
PMID: 18416563
PDF is available here.
PDF is available here.
Abstract
RNA polymerase III (Pol III) as well as Pol II (35S) promoters are able to drive hairpin RNA (hpRNA) expression and induce target gene silencing in plants. siRNAs of 21 nt are the predominant species in a 35S Pol II line, whereas 24- and/or 22-nucleotide (nt) siRNAs are produced by a Pol III line. T...
|
PMID: 18367720
PDF is available here.
PDF is available here.
Journal of Molecular Biology (378)3 2008
Abstract
We demonstrate that a Maf1 ortholog is also used to restrain pol III activity in mouse and human cells. Mammalian Maf1 represses pol III transcription in vitro and in transfected fibroblasts. Furthermore, genetic deletion of Maf1 elevates pol III transcript expression, thus confirming the role of en...
|
PMID: 18377933
PDF is available here.
PDF is available here.
Abstract
Abstract
We report a group of TRIMs (terminal-repeat retrotransposons in miniature), which are small nonautonomous retrotransposons. These elements, named Cassandra, universally carry conserved 5S RNA sequences and associated RNA polymerase (pol) III promoters and terminators in their long terminal repeats (...
|
PMID: 18408163
PDF is available here.
PDF is available here.
Abstract
Characteristics of transformed and tumor cells include increased levels of protein synthesis and elevated expression of RNA polymerase (pol) III products, such as tRNAs and 5S rRNA. However, whether deregulated pol III transcription contributes to transformation has been unclear. Generating cell lin...
|
PMID: 18394991
PDF is available here.
PDF is available here.
Abstract
Overexpression of Brf1, a transcription factor of the RNA polymerase III apparatus, can transform cells in vitro and cause tumor formation in vivo. Marshall et al. (2008) now show that one of the transcriptional products of RNA polymerase III, the initiator tRNA(Met), mediates this effect, revealing...
|
PMID: 18394985
PDF is available here.
PDF is available here.
FEBS Letters (582)5 2008
Abstract
We found that in vitro methylation blocked binding of the cellular proteins c-Myc and ATF to the 5'-region of the EBER-1 gene, and silenced the expression of the EBER-1 and EBER-2 genes, transcribed by RNA polymerase III, in transfected cells....
|
PMID: 18258198
PDF is available here.
PDF is available here.
Cellular Microbiology (10)3 2008
Abstract
We provide evidence for the existence of a unique VV infection-induced cell cycle control mechanism. The regulation is correlated with the inactivation of p53 and Rb, which are associated with the RNA polymerase III transcription factor B (TFIIIB) subunits, TBP and Brf1 respectively. VV infection in...
|
PMID: 17877750
PDF is available here.
PDF is available here.
Gene Therapy (15)3 2008
Abstract
We designed and characterized a new set of plasmid vectors driven by promoters of the Epstein-Barr virus (EBV)-encoded small RNAs (EBERs). The EBERs are the most abundant transcript in infected cells and they are transcribed by Pol III. We showed that the EBER promoters were able to drive the expres...
|
PMID: 17972920
PDF is available here.
PDF is available here.
EMBO Journal (27)1 2008
Abstract
We examined these activities and their importance in vivo. We utilized array-based methods to examine nucleosome occupancy and positioning at more than 200 locations in the genome following the controlled destruction of the catalytic subunit of RSC, Sth1. Loss of RSC function caused pronounced and g...
|
PMID: 18059476
PDF is available here.
PDF is available here.
Acta Biochimica Polonica (55)2 2008
Abstract
Maf1 was the first protein discovered to regulate polymerase III RNA in yeast and because it is evolutionarily conserved, a Maf1 ortholog also serves to restrain transcription in mouse and human cells. Understanding the mechanism of the regulation has been made possible by recent studies showing tha...
|
PMID: 18560610
PDF is available here.
PDF is available here.
Abstract
By using biochemical and reverse genetic means we show here that human RNase P is required for efficient transcription of rDNA by Pol I. Thus, inactivation of RNase P by targeting its protein subunits for destruction by RNA interference or its H1 RNA moiety for specific cleavage causes marked reduct...
|
PMID: 19115013
PDF is available here.
PDF is available here.
Abstract
Here, we report: (1) the TFIIIB subunits Brf1 and Brf2 are differentially expressed in a variety of cancer cell lines: (2) the Brf1 and Brf2 promoters differ in activity in cancer cell lines, and (3) VAI transcription is universally elevated, as compared to U6, in breast, prostate and cervical cance...
|
PMID: 18700021
PDF is available here.
PDF is available here.
Abstract
We analyzed the kinetics of transcription initiation and reinitiation of a simplified in vitro transcription system consisting only of Pol III and template DNA. The data indicates that, in the absence of transcription factors, first-round transcription initiation by Pol III proceeds at a normal rate...
|
PMID: 17965971
PDF is available here.
PDF is available here.
Journal of Gene Medicine (10)1 2008
Abstract
Thus, this new form of gene silencer 'Zorro LNA' could potentially serve as a versatile regulator of pol III-dependent transcription, including various forms of shRNAs.
(c) 2007 John Wiley & Sons, Ltd....
|
PMID: 18023071
PDF is available here.
PDF is available here.
Abstract
Small non-translated RNA genes directed by RNA polymerase III (class III genes) comprise substantial part of mammal genomes--about 10%. Besides well-known class III genes--5S rRNA, tRNA, 7SL RNA genes, mobile elements SINEs--a number of new RNA polymerase III-directed genes with poorly studied funct...
|
PMID: 18856053
PDF is available here.
PDF is available here.
Trends in Genetics (23)12 2007
Abstract
The role of RNA polymerase (Pol) III in eukaryotic transcription is commonly thought of as being restricted to a small set of highly expressed, housekeeping non-protein-coding (nc)RNA genes. Recent studies, however, have remarkably expanded the set of known Pol III-synthesized ncRNAs, suggesting tha...
|
PMID: 17977614
PDF is available here.
PDF is available here.
Abstract
We show that Staf activates U6 transcription from a preassembled chromatin template in vitro and associates with several proteins linked to chromatin modification, among them chromodomain-helicase-DNA binding protein 8 (CHD8). CHD8 binds to histone H3 di- and trimethylated on lysine 4. It resides on...
|
PMID: 17938208
PDF is available here.
PDF is available here.
Abstract
We address the question of how Pol II coordinates the expression of spliceosome components, including U6. We used chromatin immunoprecipitation (ChIP) and high-resolution mapping by PCR to localize both Pol II and Pol III to snRNA gene regions. We report the surprising finding that Pol II is highly...
|
PMID: 18039033
PDF is available here.
PDF is available here.
Abstract
We identify a causative mutation of the second largest Pol III subunit, polr3b, that disrupts digestive organ development in zebrafish slim jim (slj) mutants. The slj mutation is a splice-site substitution that causes deletion of a conserved tract of 41 amino acids in the Polr3b protein. Structural...
|
PMID: 18044988
PDF is available here.
PDF is available here.
Gene Therapy (14)21 2007
Abstract
We evaluated the strategy of using pol II promoters to drive the expression of siRNAs against HIV-1. We replaced the stem sequence in the stem-loop structure of the well-characterized miR-30a with siRNA sequences and showed that designed microRNA (miRNA) could be expressed from pol II promoters. We...
|
PMID: 17805304
PDF is available here.
PDF is available here.