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Welcome to the Pubget Medical Subject Headings (MESH) browser. Click on a topic or subtopic below to explore related papers. In the gold box is the most recent paper about the current MESH term, and below are 20 related papers.
DNA-Directed RNA Polymerases (5):
Recent article
DNA-Directed RNA Polymerases
Abstract
We have determined that the Pol II associated with the promoters of many genes has initiated transcription but paused in the region 20-50 nucleotides from the start. Here we describe the application of this method in Drosophila and human cells. The method should prove useful in assessing if promoter...
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PMID: 19272453
PDF is available here.
PDF is available here.
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Abstract
Abstract
Strategies for assembly and analysis of human, yeast, and bacterial RNA polymerase elongation complexes are described, and methods are shown for millisecond phase kinetic analyses of elongation using rapid chemical quench flow. Human, yeast, and bacterial RNA polymerases function very similarly in N...
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PMID: 19398005
PDF is available here.
PDF is available here.
Biochemical Journal (421)3 2009
Abstract
We have characterized the molecular interactions between the F/E complex and the RNAP core. F/E binds to RNAP with submicromolar affinity and is not in a dynamic exchange with unbound F/E....
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PMID: 19492989
PDF is available here.
PDF is available here.
Abstract
We compared these effects for chromosome and plasmid encoding strategies for bait proteins in two microbes: Escherichia coli and Rhodopseudomonas palustris. Differential metabolic labeling of strains expressing bait protein relative to the wild-type strain in each species allowed comparison by liqui...
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PMID: 19459691
PDF is available here.
PDF is available here.
Abstract
We demonstrate that activity against HCV NS5b polymerase is best explained using the anionic forms. The syntheses and structure-activity relationships for a variety of new analogs are also discussed....
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PMID: 19447623
PDF is available here.
PDF is available here.
Abstract
A new series of benzothiazine-substituted quinolinediones were evaluated as inhibitors of HCV polymerase NS5B. SAR studies on this series revealed a methyl sulfonamide group as a high affinity feature. Analogues with this group showed submicromolar potencies in the HCV cell based rep...
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PMID: 19457662
PDF is available here.
PDF is available here.
Methods in Molecular Biology (554)554 2009
Abstract
We used such a system to investigate the transcription termination properties of the sea urchin factor mtDBP, however, it is applicable to the study of transcription termination in a variety of organisms, provided that the pure mtRNAP and the transcription termination factor are available.The assay...
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PMID: 19513672
PDF is available here.
PDF is available here.
Abstract
Early clinical data for several nucleosides targeted to HCV Pol indicate marked antiviral effects and a likelihood of relatively slow HCV resistance, consistent with the profile of nucleosidic inhibitors of HIV and hepatitis B virus infection and supporting potentially important roles for nucleoside...
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PMID: 19426125
PDF is available here.
PDF is available here.
Virus/Uirusu (59)1 2009
Abstract
We summarize the recent accumulating information on the 3D structures of viral factors and discuss their function based on their structures....
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PMID: 19927983
PDF is available here.
PDF is available here.
Leprosy Review (80)2 2009
Abstract
Our results suggest that the use of the molecular approach for sample selection can contribute to an improvement in the quality of produced lepromin....
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PMID: 19743617
PDF is available here.
PDF is available here.
Abstract
ChemBioChem (10)7 2009
Abstract
We synthesized 6-thioguanosine 5'-diphosphate 3'-diphosphate (6-thioppGpp) as a photoaffinity probe of ppGpp; this probe thus enabled the investigation of ppGpp binding to chloroplast RNA polymerase. We found that 6-thioppGpp, as well as ppGpp, inhibits chloroplast RNA synthesis in vitro in a dose-d...
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PMID: 19308923
PDF is available here.
PDF is available here.
Nucleic Acids Research (37)8 2009
Abstract
Gene expression analysis performed through comparative abundance of transcripts is facing a new challenge with the increasing need to compare samples of known cell number, such as early embryos or laser microbiopsies, where the RNA contents of identical cellular inputs can by nature be variable. Whe...
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PMID: 19336411
PDF is available here.
PDF is available here.
Abstract
We found that the H subunit of Mg-chelatase ChlH interacts with sigma factor SigE by yeast two-hybrid screening, and immunoprecipitation analysis revealed that ChlH associates with SigE in a light-dependent manner in vivo. We also found that Mg(2+) promotes the interaction of SigE and ChlH and deter...
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PMID: 19342483
PDF is available here.
PDF is available here.
Abstract
We characterized the physiological and transcriptional responses of N. europaea cells to cyanide. The cells were extremely sensitive to low concentrations of cyanide, with NO-(2)production and ammonia-dependent oxygen uptake rates decreasing by 50% within 30 min of exposure to 1 microM NaCN. Whole-g...
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PMID: 19072843
PDF is available here.
PDF is available here.
Abstract
We report on the development of a T7 RNA polymerase-dependent expression system for B. megaterium. The system was evaluated for cytosolic and secretory protein production with green fluorescent protein (GFP) from Aequoria victoria as intracellular and Lactobacillus reuteri levansucrase as extracellu...
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PMID: 19308404
PDF is available here.
PDF is available here.
Abstract
Influenza infection is initiated by virus attachment to sialic acid-containing cell-surface receptors. The spectrum of sialylglycoconjugates varies substantially between viral host species as well as target tissues and cell types of the same species, leading to variations in the receptor-binding spe...
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PMID: 19618627
PDF is available here.
PDF is available here.
Abstract
Bacterial midrib rot of greenhouse-grown butterhead lettuce (Lactuca sativa L. var. capitata) is an emerging disease in Flanders (Belgium) and fluorescent pseudomonads are suspected to play an important role in the disease. Isolations from infected lettuces, collected from 14 commerc...
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PMID: 19157742
PDF is available here.
PDF is available here.
Abstract
Staphylococcus epidermidis is the most important pathogen in infections related to implanted foreign materials, especially prosthetic joint infections (PJIs). The aim of this study was to investigate the antimicrobial activities of 16 antibiotics against S. epidermidis isolated from PJIs, with speci...
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PMID: 19196261
PDF is available here.
PDF is available here.
Abstract
We investigated in vivo inhibitory effect of shRNA on FMDV replication in BHK-21 cells and guinea pigs. The results showed that transfection of 3D shRNA could reduce virus growth by three folds when cells were challenged with 10(2) TCID(50) of FMDV. Pretreated guinea pigs with 3DshRNA were protected...
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PMID: 18810649
PDF is available here.
PDF is available here.
EMBO Journal (28)4 2009
Abstract
We show that during stress there is a dramatic change in the nucleosome organization of stress-responsive loci that depends on Hog1 and the RSC chromatin remodelling complex. Upon stress, the MAPK Hog1 physically interacts with RSC to direct its association with the ORF of osmo-responsive genes. In...
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PMID: 19153600
PDF is available here.
PDF is available here.
Biochemical Society transactions (37)Pt 1 2009
Abstract
The recent success in reconstitution of RNAPs (RNA polymerases) from hyperthermophilic archaea from bacterially expressed purified subunits opens the way for detailed structure-function analyses of multisubunit RNAPs. The archaeal enzyme shows close structural similarity to eukaryotic RNAP, particul...
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PMID: 19143595
PDF is available here.
PDF is available here.
Biochemical Society transactions (37)Pt 1 2009
Abstract
All cellular life depends on multisubunit RNAPs (RNA polymerases) that are evolutionarily related through the three domains of life. Archaeal RNAPs encompass 12 subunits that contribute in different ways to the assembly and stability of the enzyme, nucleic acid binding, catalysis and specific regula...
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PMID: 19143594
PDF is available here.
PDF is available here.
Abstract
We report a new series of inhibitors for hepatitis C virus NS5B RNA polymerase containing a constrained pentacyclic scaffold. Our SAR studies led to the identification of hexahydroindolo[2,1-a]pyrrolo[3,2-d][2]benzazepines exposing basic groups. The compounds displayed a high activity in the enzyme...
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PMID: 19109015
PDF is available here.
PDF is available here.