Find your PDFs fast.
Welcome to the Pubget Medical Subject Headings (MESH) browser. Click on a topic or subtopic below to explore related papers. In the gold box is the most recent paper about the current MESH term, and below are 20 related papers.
RNA Nucleotidyltransferases (7):
Recent article
RNA Nucleotidyltransferases
Abstract
Concurrent andrographolide treatment (10 microM, 3 h) sensitized Ras-transformed cells to radiation in vitro (sensitizer enhancement ratio, 1.73). Andrographolide plus radiation (one dose of 300 mg/kg peritumor andrographolide and one dose of 6 Gy radiation) resulted in significant tumor growth dela...
|
PMID: 20610044
PDF is available here.
PDF is available here.
| < More recent | Older article > |
Journal of Bacteriology (191)1 2009
Abstract
The genome of Geobacter sulfurreducens contains three genes whose sequences are quite similar to sequences encoding known members of an RNA nucleotidyltransferase superfamily that includes tRNA nucleotidyltransferases and poly(A) polymerases. Reverse transcription-PCR using G. sulfurreducens total R...
|
PMID: 18952795
PDF is available here.
PDF is available here.
Genome Research (18)10 2008
Abstract
We speculate that mRNA target site-directed editing of mmu-let-7a duplex-bulges stabilizes "loose" miRNA:mRNA target associations and functions to expand the target repertoire and/or enhance mRNA decay over translational repression. Our results also demonstrate that the systematic study of sequence...
|
PMID: 18614752
PDF is available here.
PDF is available here.
Abstract
We present here the details of a general approach to fluorescent labeling of the CCA sequence with the fluorescent base analog pyrrolo-C (PyC) at position 75 as a molecular probe for monitoring the dynamics of the tRNA 3' end. Using Escherichia coli tRNA(Cys) as an example, we achieve such labeling...
|
PMID: 18755841
PDF is available here.
PDF is available here.
Journal of Lipid Research (49)10 2008
Abstract
Change in phosphoethanolamine pool size in tumor tissues is an important indicator of tumor prognosis and drug therapy efficacy. Phosphoethanolamine is the substrate of the regulatory enzyme CTP:phosphoethanolamine cytidylyltransferase (ECT) in the de novo biosynthesis of phosphatidylethanolamine (P...
|
PMID: 18583706
PDF is available here.
PDF is available here.
Nucleic Acids Research (36)16 2008
Abstract
Showing a high sequence similarity, the evolutionary closely related bacterial poly(A) polymerases (PAP) and CCA-adding enzymes catalyze quite different reactions--PAP adds poly(A) tails to RNA 3'-ends, while CCA-adding enzymes synthesize the sequence CCA at the 3'-terminus of tRNAs. Here, two highl...
|
PMID: 18682528
PDF is available here.
PDF is available here.
Journal of Cell Biology (182)3 2008
Abstract
We have investigated in Caenorhabditis elegans whether Dhh1 and Pat1 generally function together, and how they influence mRNA sequestration during oogenesis. We show that in somatic tissues, the Dhh1 orthologue (CGH-1) forms Pat1 (patr-1)-dependent P bodies that are involved in mRNA decapping. In co...
|
PMID: 18695045
PDF is available here.
PDF is available here.
2'-5' oligoadenylate synthetase shares active site architecture with the archaeal CCA-adding enzyme.
Abstract
We compared the structure of OAS to that of poly adenosine polymerase (PAP) and the class I CCA-adding enzyme from Archeoglobus fulgidus (AfCCA). This comparison revealed a strong structural homology between the three enzyme families. In particular, the active sites of OAS and CCA class I enzymes ar...
|
PMID: 18604630
PDF is available here.
PDF is available here.
EMBO Journal (27)14 2008
Abstract
We present almost a dozen complex structures of the class I CCA-adding enzyme and tRNA mini-helices (mini-D(73)N(74), mini-D(73)N(74)C(75) and mini-D(73)C(74)N(75); D(73) is a discriminator nucleotide and N is either A, G, or U). The mini-D(73)N(74) complexes adopt catalytically inactive open forms,...
|
PMID: 18583961
PDF is available here.
PDF is available here.
Abstract
We performed a mutational analysis to dissect the role of this residue in CCA-addition activity. We found that the phylogenetically permissible P295G mutant and the phylogenetically absent P295T had little effect on CCA addition, whereas P295A and P295S progressively interfered with CCA addition (C7...
|
PMID: 18495940
PDF is available here.
PDF is available here.
Abstract
CCA-adding enzymes are specialized polymerases that add a specific sequence (C-C-A) to tRNA 3' ends without requiring a nucleic acid template. In some organisms, CCA synthesis is accomplished by the collaboration of evolutionary closely related enzymes with partial activities (CC and A addition). Th...
|
PMID: 18523015
PDF is available here.
PDF is available here.
Journal of Molecular Biology (379)3 2008
Abstract
We show here by single-turnover kinetics that minihelices are insufficient substrates for the Escherichia coli CCA enzyme and that only the full-length tRNA is kinetically competent. Even a nick in the full-length tRNA backbone in the T loop, or as far away from the minihelix domain as in the antico...
|
PMID: 18466919
PDF is available here.
PDF is available here.
Abstract
Abstract
Representing one of the most fascinating RNA polymerases, the CCA-adding enzyme (tRNA nucleotidyltransferase) is responsible for synthesis and repair of the 3'-terminal CCA sequence in tRNA transcripts. As a consequence of this important function, this enzyme is found in all organisms analyzed so fa...
|
PMID: 18226598
PDF is available here.
PDF is available here.
Abstract
We searched Chlamydomonas for open reading frames (ORFs) potentially encoding exoribonucleases, poly(A) polymerases, and proteins known to associate with and/or regulate them. The ORFs were further analyzed for indications of protein localization to the nucleus, cytosol, mitochondrion, and/or chloro...
|
PMID: 18493045
PDF is available here.
PDF is available here.
Biochimica et Biophysica Acta (1779)4 2008
Abstract
Poly(A) polymerases were identified almost 50 years ago as enzymes that add multiple AMP residues to the 3' ends of primer RNAs without use of a template from ATP as cosubstrate and with release of pyrophosphate. Based on sequence homology of a signature motif in the catalytic domain, poly(A) polyme...
|
PMID: 18177750
PDF is available here.
PDF is available here.
Biochimica et Biophysica Acta (1779)4 2008
Abstract
Abstract
We show that a single guanine-to-adenine transition in cca1-1 generates the temperature-sensitive phenotype. Alignment of the amino acid sequence of S. cerevisiae tRNA nucleotidyltransferase with other tRNA nucleotidyltransferases for which crystal structures have been solved suggests that the resul...
|
PMID: 18302315
PDF is available here.
PDF is available here.
Abstract
We describe here an alternative assay involving [3'-32P]-labeled tRNA followed by nuclease digestion and TLC analysis that permits aminoacylation to be monitored in an efficient, quantitative manner while circumventing many of the problems faced when using radiolabeled amino acids. We also describe...
|
PMID: 18241789
PDF is available here.
PDF is available here.
Abstract
RNA-specific nucleotidyl transferases (rNTrs) are a diverse family of template-independent polymerases that add ribonucleotides to the 3'-ends of RNA molecules. All rNTrs share a related active-site architecture first described for DNA polymerase beta and a catalytic mechanism conserved among DNA an...
|
PMID: 17872511
PDF is available here.
PDF is available here.
Abstract
We propose a nucleotide synthetase ribozyme as an alternative candidate, especially considering recent experimental evidence suggesting the possibility of effective nonenzymatic template-directed synthesis of RNA. A computer simulation was conducted to support our proposal. The conditions for the em...
|
PMID: 17878321
PDF is available here.
PDF is available here.
Animal Reproduction Science (101)3-4 2007
Abstract
The vasa gene, first described in Drosophila, is purported to be important in germ cell development. Vasa is present across several invertebrate and vertebrate taxa, including frogs, fish, chickens, and humans. Vasa, a DEAD (asparagine-glutamine-alanine-asparagine) box protein shown to function as a...
|
PMID: 17150314
PDF is available here.
PDF is available here.
Abstract
We have previously determined crystal structures of the UTP-bound and apo forms of the minimal trypanosomal TUTase, TbTUT4, which is composed solely of the N-terminal catalytic and C-terminal base-recognition domains. Here we report crystal structures of TbTUT4 with bound CTP, GTP, and ATP, demonstr...
|
PMID: 17785418
PDF is available here.
PDF is available here.
Abstract
We have used deoxyribozyme-synthesized 2',5'-branched RNA corresponding exactly to the proposed Ty1 branch site for a direct test of this read-through ability. Using an in vitro assay that incorporates all components known to be required for Ty1 cDNA synthesis (including the TyA chaperone protein),...
|
PMID: 17652136
PDF is available here.
PDF is available here.
Abstract
We describe properties of the mouse and human Pcyt2 genes and their regulatory promoters and provide molecular evidence for the existence of 2 distinct Pcyt2 proteins. The goal is to obtain more insight into Pcyt2 catalytic function and regulation to facilitate a better understanding of the producti...
|
PMID: 17612623
PDF is available here.
PDF is available here.
Abstract
Abstract
We have disrupted the mouse gene encoding CTP:phosphoethanolamine cytidylyltransferase, Pcyt2, the main regulatory enzyme in this pathway. Intercrossings of Pcyt2(+/-) animals resulted in small litter sizes and unexpected Mendelian frequencies, with no null mice genotyped. The Pcyt2(-/-) embryos die...
|
PMID: 17325045
PDF is available here.
PDF is available here.
Genome Research (17)4 2007
Abstract
The prediction of gene models from genome sequence remains an unsolved problem. One hallmark of eukaryotic gene structure is the presence of introns, which are spliced out of pre-mRNAs prior to translation. The excised introns are released in the form of lariats, which must be debranched prior to th...
|
PMID: 17351133
PDF is available here.
PDF is available here.
Journal of Molecular Biology (366)4 2007
Abstract
We describe the sequence and activity of a splice variant of the human CCA-adding enzyme identified in public cDNA databases. The in silico analyses performed on this splice variant indicate that there is conservation of the alternative splice donor site among species and indicate that it seems to b...
|
PMID: 17204286
PDF is available here.
PDF is available here.
Journal of Molecular Biology (366)3 2007
Abstract
We report identification, structural and biochemical analyses of a novel trypanosomal TUTase, TbTUT4, which represents a minimal catalytically active RNA uridylyltransferase. The TbTUT4 consists of only two domains that define the catalytic center at the bottom of the nucleoside triphosphate and RNA...
|
PMID: 17189640
PDF is available here.
PDF is available here.
Abstract
We report the characterization of the yeast Npa2p (Urb2p) protein, which is essential for 60S ribosomal subunit biogenesis. We identified this protein in a synthetic lethal screening with the rsa3 null allele. Rsa3p is a genetic partner of the putative RNA helicase Dbp6p. Mutation or depletion of Np...
|
PMID: 17145778
PDF is available here.
PDF is available here.
Abstract
Based on the close evolutionary relation of these two nucleotidyltransferases, it was tested whether Hfq is a specific modulator acting exclusively on PAP or whether it also influences the activity of the CCA-adding enzyme. The obtained data indicate that the reaction catalyzed by this enzyme is sub...
|
PMID: 17949481
PDF is available here.
PDF is available here.
Abstract
These results demonstrate that glycerophospholipid synthesis is very active in the G2/M phase of these cells. Therefore, the postulated cessation of phospholipid synthesis in G2/M phases is not applicable to all cell types....
|
PMID: 17113814
PDF is available here.
PDF is available here.
Abstract
We have solved the crystal structure of the tRNA-maturating enzymes in a complex with tRNA (precursor) to especially elucidate the sophisticated mechanism of their highly specific chemical reactions. Our goal is to provide the animated crystallography or "movie" of the dynamic processes in the genet...
|
PMID: 18029606
PDF is available here.
PDF is available here.
Methods in Enzymology (424) 2007
Abstract
Terminal RNA uridylyltransferases (TUTases) catalyze the transfer of UMP residues to the 3' hydroxyl group of RNA. These enzymes belong to the DNA polymerase beta superfamily, which also includes poly(A) polymerases, CCA-adding enzymes, and other nucleotidyltransferases. Studies of uridylyl insertio...
|
PMID: 17662836
PDF is available here.
PDF is available here.
Abstract
Enzymes involved in tRNA maturation are essential for cytosolic, mitochondrial, and plastid protein synthesis and are therefore localized to these different compartments of the cell. Interestingly, only one isoform of tRNA nucleotidyltransferase (responsible for adding the 3'-terminal cytidine-cytid...
|
PMID: 18182422
PDF is available here.
PDF is available here.
Abstract
We introduced mutations designed to reverse the polarity of hydrogen bonds between the nucleobases and protein template. We were able to transform the CCA-adding enzyme into a (U,G)-adding enzyme that incorporates UTP and GTP instead of CTP and ATP; we transformed the related Aquifex aeolicus CC- an...
|
PMID: 17179213
PDF is available here.
PDF is available here.
Plant Cell Online (18)12 2006
Abstract
A TILLING strategy (for targeting-induced local-scale lesions in genomes) was used in Arabidopsis thaliana to isolate mutants of a gene encoding CTP:PHOSPHORYLETHANOLAMINE CYTIDYLYLTRANSFERASE (PECT; EC 2.7.7.14), a rate-limiting enzyme in phosphatidylethanolamine biosynthesis. A null mutation, pect...
|
PMID: 17189343
PDF is available here.
PDF is available here.
Abstract
CTP:phosphoethanolamine cytidylyltransferase (ECT) is the enzyme that catalyzes the conversion of phosphoethanolamine to CDP-ethanolamine in the phosphatidylethanolamine-biosynthetic pathway (Kennedy pathway). ECT from Saccharomyces cerevisiae was crystallized by the sitting-drop vapour-diffusion me...
|
PMID: 17012796
PDF is available here.
PDF is available here.
DNA Sequence (17)4 2006
Abstract
We characterized Bat1a and Nfkbil1 in inbred mice differing in their H2 haplotype. We identified two indels and nine single nucleotide polymorphisms (SNP) upstream of Nfkbil1, one indel, nine SNP upstream of Bat1a and a synonymous SNP in exon 2 of Bat1a. H2(g7) and H2(b) mice yielded identical Bat1a...
|
PMID: 17312949
PDF is available here.
PDF is available here.
Molecular Cell (23)3 2006
Abstract
We find evidence that the GTPase Snu114p mediates the regulation of spliceosome activation and disassembly. Specifically, both unwinding of U4/U6, required for spliceosome activation, and disassembly of the postsplicing U2/U6.U5.intron complex are repressed by Snu114p bound to GDP and derepressed by...
|
PMID: 16885028
PDF is available here.
PDF is available here.
Abstract
Mammalian cells contain a highly specific terminal uridylyl transferase (TUTase) that exclusively accepts U6 snRNA as substrate. This enzyme, termed U6-TUTase, was purified from HeLa cell extracts and analyzed by microsequencing. All sequenced peptides matched a unique human cDNA coding for a previo...
|
PMID: 16790842
PDF is available here.
PDF is available here.
Abstract
rck/p54, a DEAD-box RNA helicase, is closely associated with the basic modification of RNA molecules in the process of mRNA transport, RNA decay, and translation initiation. In the current study, Western blot analysis revealed that rck/p54 protein was ubiquitously expressed in mouse tissues. Interes...
|
PMID: 16618942
PDF is available here.
PDF is available here.
Genes to Cells (11)4 2006
Abstract
I formed a closed conformation, which was induced by Asn131, a residue unique to the RCK subfamily. It appears that ATP does not bind to the P-loop. The results of dynamic light scattering revealed to ATP-induced conformational change of rck/p54. It was demonstrated that free rck/p54 is a distended...
|
PMID: 16611246
PDF is available here.
PDF is available here.
Journal of Biological Chemistry (281)14 2006
Abstract
We found previously that tRNA does not rotate or translocate on the enzyme during the addition of C75 and A76. We therefore predicted that the growing 3'-end of tRNA must, upon addition of each nucleotide, refold to reposition the new 3'-hydroxyl equivalently relative to the solitary nucleotidyltran...
|
PMID: 16455665
PDF is available here.
PDF is available here.
Microbiology (152)Pt 3 2006
Abstract
These results strongly suggest that the enzyme responsible for RNA 3'-tail synthesis in S. coelicolor and other streptomycetes is polynucleotide phosphorylase (PNPase). Moreover, this study shows that both PNPase and the product of SCO3896 are essential. It is possible that the dual functions of PNP...
|
PMID: 16514143
PDF is available here.
PDF is available here.
Abstract
We here demonstrate that TbMP57 TUTase of U-insertion can act efficiently within a U-deletion cycle. When physiological UTP levels are provided, it adds U's to the upstream cleavage fragment after U-deletional endonuclease and 3'-U-exo action, but before rejoining by the U-deletional ligase, generat...
|
PMID: 16495238
PDF is available here.
PDF is available here.
Abstract
I CCA-adding enzyme employs both an arginine sidechain and backbone phosphates of the bound tRNA to recognize incoming nucleotides. It switches from C to A addition through changes in the size and shape of the nucleotide-binding pocket, which is progressively altered by the elongating 3' terminus of...
|
PMID: 16364630
PDF is available here.
PDF is available here.
Nucleic Acids Research (34)15 2006
Abstract
I shall give a general overview of the DEAD-box protein family....
|
PMID: 16936318
PDF is available here.
PDF is available here.
Nucleic Acids Research (34)10 2006
Abstract
The DEAD-box RNA helicase Xp54 is an integral component of the messenger ribonucleoprotein (mRNP) particles of Xenopus oocytes. In oocytes, several abundant proteins bind pre-mRNA transcripts to modulate nuclear export, RNA stability and translational fate. Of these, Xp54, the mRNA-masking protein F...
|
PMID: 16769775
PDF is available here.
PDF is available here.
Abstract
La synthèse résiduelle de RNA dans le foie de rats soumis à une injection de lasiocarpine a été étudiée par autoradiographie à haute résolution après administration d'uridine tritiée. Les blocs ont été débarrassés autant que possible des précurseurs acido-solubles marqués du RNA, et...
PDF is available here.
PDF is available here.
Molecular Cell (20)6 2005
Abstract
We identify three proteins that exist in complex with the decapping enzyme subunits hDcp2 and hDcp1: hEdc3, Rck/p54, and a protein in decapping we name Hedls. Hedls is important in decapping because it enhances the activity of the catalytic hDcp2 subunit and promotes complex formation between hDcp2...
|
PMID: 16364915
PDF is available here.
PDF is available here.
EMBO Journal (24)23 2005
Abstract
We report the 1.8 A crystal structure of the Trypanosoma brucei RET2 apoenzyme and its complexes with uridine nucleotides. This structure reveals that the specificity of the TUTase for UTP is determined by a crucial water molecule that is exquisitely positioned by the conserved carboxylates D421 and...
|
PMID: 16281058
PDF is available here.
PDF is available here.
Abstract
We have generated a set of plasmids for dsRNA-mediated knockdown of DBR under diverse conditions in transgenic Drosophila and in cultured cells. We describe the use of these plasmids and protocols for lariat analysis. We have generated transgenic Drosophila strains carrying a GAL4-regulated RNAi con...
|
PMID: 16314266
PDF is available here.
PDF is available here.
Journal of Biological Chemistry (280)45 2005
Abstract
We report that mice that lack both PEMT and MDR2 and are fed a CD diet survive for >90 days. Unexpectedly, the amount of PC also decreases by 50% in the livers of Mdr2(-/-)/Pemt(-/-) mice. The Mdr2(-/-)/Pemt(-/-) mice adapt to the severe choline deprivation via choline recycling by induction of phos...
|
PMID: 16144842
PDF is available here.
PDF is available here.
Abstract
Polynucleotide polymerases play a crucial role in transmitting genetic information from generation to generation, and they are the most important reagents in biotechnology. Although classical crystal structure analyses as well as biochemical studies have significantly contributed to our understandin...
|
PMID: 16143831
PDF is available here.
PDF is available here.
Development (132)22 2005
Abstract
We describe a predicted RNA-binding protein, CAR-1, that associates with CGH-1 and Y-box proteins within a conserved germline RNA-protein (RNP) complex, and in cytoplasmic particles in the gonad and early embryo. The CGH-1/CAR-1 interaction is conserved in Drosophila oocytes. When car-1 expression i...
|
PMID: 16221731
PDF is available here.
PDF is available here.
Journal of Cell Biology (171)2 2005
Abstract
We characterize CAR-1, a predicted RNA binding protein that is implicated in cytokinesis. CAR-1 localizes to germline-specific RNA-containing particles and copurifies with the essential RNA helicase, CGH-1, in an RNA-dependent fashion. The atypical Sm domain of CAR-1, which directly binds RNA, is di...
|
PMID: 16247027
PDF is available here.
PDF is available here.
Molecular Cell (20)1 2005
Abstract
We report that depletion of the DEAD box helicase Dbp4p dramatically increased cosedimentation of the snoRNAs U14 and snR41 with preribosomes. Cosedimentation was maintained after deproteinization by proteinase K, indicating that the snoRNAs remained base paired to the pre-rRNA. Affinity purificatio...
|
PMID: 16209945
PDF is available here.
PDF is available here.
Cellular and Molecular Life Sciences (62)19-20 2005
Abstract
I discuss the current knowledge of this novel enzyme family and possible roles of RNA uridylylation in the regulation of gene expression....
|
PMID: 16158189
PDF is available here.
PDF is available here.