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Nucleotidyltransferases (16):
- UDPglucose-Hexose-1-Phosphate Uridylyltransferase
- Rec A Recombinases
- 2',5'-Oligoadenylate Synthetase
- UTP-Hexose-1-Phosphate Uridylyltransferase
- RNA Nucleotidyltransferases
- DNA Nucleotidyltransferases
- Transposases
- Polynucleotide Adenylyltransferase
- Nicotinamide-Nucleotide Adenylyltransferase
- Sulfate Adenylyltransferase
- VDJ Recombinases
- Choline-Phosphate Cytidylyltransferase
- Glucose-1-Phosphate Adenylyltransferase
- UTP-Glucose-1-Phosphate Uridylyltransferase
- Transposon Resolvases
- N-Acylneuraminate Cytidylyltransferase
Recent article
Nucleotidyltransferases
Abstract
We demonstrate that the resistance enzyme ANT(4') from Staphylococcus aureus is highly substrate and cosubstrate promiscuous. We show that deoxy-ribonucleotide triphosphates (dNTPs) are better cosubstrates than NTPs. We demonstrate that the position of the triphosphate group (5' and not 3') on the r...
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PMID: 21040710
PDF is available here.
PDF is available here.
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Abstract
We here generated a mouse Rev1 monoclonal antibody, called D6, which also recognizes human REV1. The epitope for the antibody could be mapped between 860-877 amino acid residues of human REV1, which was located outside of the conserved catalytic domain. Although the antibody unfortunately could not...
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PMID: 21265264
PDF is available here.
PDF is available here.
Abstract
Our studies using RNA cRACE (circularization-based rapid amplification of cDNA ends) techniques indicate that mRNA decapping in the fission yeast Schizosaccharomyces pombe often does not require prior deadenylation. Furthermore, the poly(A) polymerase-related, cytoplasmic enzyme Cid1 catalyses uridy...
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PMID: 20659020
PDF is available here.
PDF is available here.
Abstract
We performed Brownian dynamics simulations of the R.P(c) --> R.P(o) transition. In the fast trajectories, unwinding of the promoter DNA begins by local melting around the -10 element, which is followed by sequential unzipping of DNA till the +2 site. The R.P(c) --> R.P(o) transition occurs in three...
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PMID: 20615963
PDF is available here.
PDF is available here.
Endocrinology (151)7 2010
Abstract
Elevated glucose and saturated fatty acids synergize in inducing apoptosis in INS832/13 cells and in human islet cells. In order to gain insight into the molecular mechanism(s) of glucolipotoxicity (Gltox), gene profiling and metabolic analyses were performed in INS832/13 cells cultured at 5 or 20 m...
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PMID: 20444946
PDF is available here.
PDF is available here.
Abstract
We used a model system of purified components to explore the effects of a downstream target on the signaling properties of a covalent modification cycle, an example of retroactivity. In the experimental system used, a bifunctional enzyme catalyzed the modification and demodification of its substrate...
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PMID: 20479260
PDF is available here.
PDF is available here.
Abstract
We found that the increase of telomere length extended organismic lifespan in Caenorhabditis elegans. To investigate the effect of the telomere length on organismic lifespan at the global gene expression level, we performed microarray experiments using mRNA from long telomere-containing worms. We id...
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PMID: 20430011
PDF is available here.
PDF is available here.
Abstract
We developed a phosphate sensor-based high-throughput screening assay to quantify the activity of GDP-MP and screened a library containing approximately 80,000 lead-like compounds for GDP-MP inhibitors. On the basis of their GDP-MP inhibitory properties and chemical structures, the activities of 20...
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PMID: 20160053
PDF is available here.
PDF is available here.
Abstract
When a voltage is imposed across a thin membrane containing a nanoscopic pore, the electric field generated within the pore captures linear ionized polymers, such as nucleic acids, that are present in the solution bathing the pore. The nucleic acid molecule transiently blocks ionic current as it is...
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PMID: 20192777
PDF is available here.
PDF is available here.
Journal of Molecular Biology (396)3 2010
Abstract
We previously reported the three-dimensional structure of the AR domain (residues 1-440). The present structure contains both the R and AT domains. AR and AT share 24% sequence identity and also contain the beta-polymerase motif that is characteristic of many nucleotidylyl transferase enzymes. The s...
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PMID: 20026075
PDF is available here.
PDF is available here.
Abstract
These results show that SlJAR1 synthesizes the bioactive conjugate (+)-7-iso-JA-Ile and this is the predominant isomer accumulated in wounded tomato leaves....
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PMID: 20012084
PDF is available here.
PDF is available here.
Abstract
We show that archaeal homologs of Thg1 catalyze G(-1) addition, in vitro and in vivo in yeast, but only in a templated reaction, i.e. with tRNA(His) substrates that contain a C(73) discriminator nucleotide. Because tRNA(His) from archaea contains C(73), these findings are consistent with a physiolog...
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PMID: 20080734
PDF is available here.
PDF is available here.
Abstract
We report on an elicitor obtained from the green algae Ulva spp. A fraction containing most exclusively the sulfated polysaccharide known as ulvan-induced expression of a GUS gene placed under the control of a lipoxygenase gene promoter. Gene expression profiling was performed upon ulvan treatments...
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PMID: 20445752
PDF is available here.
PDF is available here.
Abstract
AmtR, a member of the TetR family of transcription regulators, is a global regulator of nitrogen control in Corynebacterium glutamicum. Unlike other TetR-family members, which are regulated by small-molecule effectors, AmtR is regulated by a protein called GlnK. It has been shown that a GlnK trimer...
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PMID: 19923732
PDF is available here.
PDF is available here.
Abstract
There were 30 bp and 8 bp unique nucleotide in ITS and matK sequence in Forsythia suspensa. The matK gene and ITS sequences might be good molecular marker to be used to identify the original plant of Forsythia suspensa. CONCLUSION: The sequence analysis of matK gene ITS sequences might become the me...
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PMID: 20112711
PDF is available here.
PDF is available here.
Genetika (Moskva) (45)10 2009
Abstract
We identified one, which differed from other regulatory mutants by overproduction of riboflavin and simultaneous upregulation of the rib C gene encoding flavokinase/FAD-synthase. Genetic and biochemical analysis showed that the ribU1 mutation determines a trans-acting factor that simultaneously regu...
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PMID: 19947554
PDF is available here.
PDF is available here.
Abstract
We show here that many of the key elements of this highly unusual strategy are conserved between yeast and human Rev1, including the eviction of template G from the DNA helix and the pairing of incoming deoxycytidine 5'-triphosphate with a surrogate arginine residue. We also show that the catalytic...
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PMID: 19464298
PDF is available here.
PDF is available here.
Biochemistry (Washington) (48)25 2009
Abstract
We show that ORF MJ1117 of the hyperthermophilic, methanogenic archaeon Methanocaldococcus jannaschii encodes a CobY protein (Mj CobY) that transfers the GMP moiety of GTP to AdoCbi-P to form AdoCbi-GDP. Results from isothermal titration calorimetry (ITC) experiments show that MjCobY binds GTP (K(d)...
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PMID: 19489548
PDF is available here.
PDF is available here.
Genes & Development (23)12 2009
Abstract
We determine if the Mec1 kinase and the subunits of the checkpoint clamp and the clamp loader are required for TLS. We show that proficient TLS can occur in the absence of these checkpoint proteins in nucleotide excision repair (NER)-proficient cells; however, in the absence of NER, checkpoint prote...
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PMID: 19528320
PDF is available here.
PDF is available here.
Abstract
These results indicate that the CDP-D: -glucose synthase encoded by ste17 gene is involved in the formation of nucleotide sugar (CDP-D-glucose) as glucose precursor in Ebosin biosynthesis....
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PMID: 19330326
PDF is available here.
PDF is available here.
Abstract
We present an analysis of the chemical function of a recombinant bifunctional phosphomannose isomerase/GDP-mannose pyrophosphorylase (manC) from Pyrococcus furiosus DSM 3638 and its use in the synthesis of guanidinediphospho-hexoses and a range of nucleotidediphospho-mannoses. This enzyme is unusual...
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PMID: 19421452
PDF is available here.
PDF is available here.
Zhonghua Yixue Zazhi (89)19 2009
Abstract
The expressions of 4 genes including Rev3, Rev7, Pol eta and Pol iota were significantly (P < 0.05) higher in glioma tissues in grade II, III and IV as compared to normal brain tissues. The expressions of Rev1 and Pol kappa were significantly (P < 0.05) higher in grade IV glioma compared to the norm...
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PMID: 19615182
PDF is available here.
PDF is available here.
Abstract
We analysed the mutation spectrum of somatically mutated immunoglobulin genes in B cells from PCNAK164R knock-in mice. A 10-fold reduction in A/T mutations is associated with a compensatory increase in G/C mutations-a phenotype similar to Poleta and mismatch repair-deficient B cells. Mismatch recogn...
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PMID: 19008189
PDF is available here.
PDF is available here.
Abstract
Abstract
We show that the proton donor to pyrophosphate is an active-site amino acid residue. The use of general acid catalysis by polymerases extends the mechanism of nucleotidyl transfer beyond that of the well-established two-metal-ion mechanism. The existence of an active-site residue that regulates poly...
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PMID: 19151724
PDF is available here.
PDF is available here.
Abstract
We show that the enzyme can be reconstituted from two purified polypeptides that comprise the N-terminal two-thirds of the protein and the C-terminal one-third of the protein. Properties of the reconstituted enzyme support recent hypotheses for the sites of regulatory interactions and mechanisms for...
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PMID: 19105634
PDF is available here.
PDF is available here.
Abstract
We isolated a pol inhibitor from the cultured mycelia extract of a fungal strain isolated from natural salt from a sea salt pan in Australia, which was identified as 3-O-methylfunicone by spectroscopic analyses. This compound selectively inhibited the activities of mammalian Y-family DNA polymerases...
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PMID: 20098603
PDF is available here.
PDF is available here.
Abstract
Abstract
These results indicate that specialized DNA polymerases are involved in the mutagenesis induced by 8-OH-dGTP....
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PMID: 19749341
PDF is available here.
PDF is available here.
Acta Crystallographica Section D (64)Pt 12 2008
Abstract
The enzyme 3-deoxy-manno-octulosonate cytidylyltransferase (CMP-KDO synthetase; CKS) catalyzes the activation of 3-deoxy-D-manno-octulosonate (or 2-keto-3-deoxy-manno-octonic acid; KDO) by forming CMP-KDO. CKS is unique to Gram-negative bacteria and is an attractive target for the development of ant...
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PMID: 19018107
PDF is available here.
PDF is available here.
Abstract
We report that mutations in the Arabidopsis gene encoding GDP-mannose pyrophosphorylase (GMPase) essential for synthesizing GDP-mannose confer hypersensitivity to NH(4)(+). The in planta activities of WT and mutant GMPases all were inhibited by NH(4)(+), but the magnitude of the inhibition was signi...
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PMID: 19011088
PDF is available here.
PDF is available here.
Journal of Biological Chemistry (283)45 2008
Abstract
We named AtRibF1 and AtRibF2, carry N-terminal extensions with characteristics of organellar targeting peptides. AtRibF1 and AtRibF2 cDNAs were cloned by reverse transcription-PCR. Only FAD synthetase activity was detected in the recombinant enzymes produced in Escherichia coli. FMN and ATP inhibite...
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PMID: 18713732
PDF is available here.
PDF is available here.
Abstract
We conducted a mutational analysis of the vaccinia virus lid peptide ((545)DKFRLNPEVSYFTNKRTRG(563)) entailing in vivo and in vitro readouts of the effects of alanine and conservative substitutions. We thereby identified essential functional groups that interact with the AdoMet sulfonium (Tyr555, Ph...
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PMID: 18799596
PDF is available here.
PDF is available here.
Journal of Bacteriology (190)21 2008
Abstract
We found that the OmpR-type regulator protein GlnR controls nitrogen-dependent transcription regulation in Mycobacterium smegmatis. Based on RNA hybridization experiments with a wild-type strain and a corresponding mutant strain, real-time reverse transcription-PCR analyses, and DNA binding studies...
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PMID: 18689485
PDF is available here.
PDF is available here.
Journal of Bacteriology (190)21 2008
Abstract
We identified a cluster of polysaccharide biosynthesis genes (hfsEFGH) directly adjacent to the holdfast polysaccharide export genes. Sequence analysis indicated that these genes are involved in the biosynthesis of the minimum repeat unit of the holdfast polysaccharide. HfsE is predicted to be a UDP...
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PMID: 18757530
PDF is available here.
PDF is available here.
Abstract
Addition of a 5' cap to RNA polymerase II transcripts, the first step of pre-mRNA processing in eukaryotes from yeasts to mammals, is catalyzed by the sequential action of RNA triphosphatase, guanylyltransferase, and (guanine-N-7)methyltransferase. The effects of knockdown of these capping enzymes i...
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PMID: 18678651
PDF is available here.
PDF is available here.
Abstract
The presence of a common insertion point for isolates from different geographical areas could suggest the insertion of Tn1546 by transposition in a plasmid-specific site, followed by genetic rearrangement both inside the transposon and in the flanking regions....
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PMID: 18583326
PDF is available here.
PDF is available here.
Journal of Biological Chemistry (283)37 2008
Abstract
We investigated the involvement of the human L-cysteine desulfurase Nfs1 in sulfur transfer to MOCS3-RLD. A variant of Nfs1 was purified in conjunction with Isd11 in a heterologous expression system in Escherichia coli, and the kinetic parameters of the purified protein were determined. By studying...
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PMID: 18650437
PDF is available here.
PDF is available here.
Abstract
The gene product of open reading frame Rv1018c from Mycobacterium tuberculosis is annotated as encoding a probable N-acetylglucosamine 1-phosphate uridylyltransferase (MtbGlmU), an enzyme that catalyzes the biosynthesis of UDP-N-acetylglucosamine, a precursor common to lipopolysaccharide and peptido...
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PMID: 18765909
PDF is available here.
PDF is available here.
Microbiology (154)Pt 9 2008
Abstract
Our results showed that the Afsrb1 gene is essential for cell wall integrity, morphogenesis and viability of Aspergillus fumigatus....
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PMID: 18757806
PDF is available here.
PDF is available here.
Plant Cell Reports (27)9 2008
Abstract
We established a procedure to prepare sporangia of M. polymorpha under laboratory conditions by promoting its transition to reproductive development by far-red light irradiation. Here we report an improved direct transformation system of M. polymorpha using immature thalli developing from spores. Hy...
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PMID: 18553085
PDF is available here.
PDF is available here.
Abstract
Plant Physiology (148)1 2008
Abstract
We have characterized a viable Arabidopsis (Arabidopsis thaliana) T-DNA mutant affected in the penultimate step of the CoA biosynthesis pathway, which is catalyzed by the enzyme phosphopantetheine adenylyltransferase (PPAT). This ppat-1 knockdown mutation showed an approximately 90% reduction in PPA...
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PMID: 18621975
PDF is available here.
PDF is available here.
Journal of Virology (82)18 2008
Abstract
We now find that the capping-associated enzymatic activities of recombinant nsP1, containing ts10 or ts14 lesions, were not ts. The mutant proteins and polyproteins also were membrane bound, mutant nsP1 interacted normally with the other nonstructural proteins, and there was no major defect in nonst...
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PMID: 18596091
PDF is available here.
PDF is available here.
Journal of Virology (82)18 2008
Abstract
We show that this enzyme also catalyzes nontemplated nucleotide additions to single-stranded and double-stranded nucleic acid molecules. This terminal nucleotidyltransferase activity not only is a property of the isolated enzyme but also is detected to take place within the viral nucleocapsid. This...
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PMID: 18614640
PDF is available here.
PDF is available here.
Abstract
We provide evidence for the requirement of Rad5 in TLS mediated by Polzeta. Using duplex plasmids carrying different site-specific DNA lesions--an abasic site, a cis-syn TT dimer, a (6-4) TT photoproduct, or a G-AAF adduct--we show that Rad5 is needed for Polzeta-dependent TLS. Rad5 action in this r...
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PMID: 18757916
PDF is available here.
PDF is available here.
Biochimica et Biophysica Acta (1777)9 2008
Abstract
We used two mutants of Synechocystis sp. PCC6803: the PAL mutant which has no phycobilisomes and shows a high PSII/PSI ratio, and a mutant derived from it by inactivating its cdsA gene encoding cytidine 5'-diphosphate diacylglycerol synthase, a key enzyme in PG synthesis. In a medium supplemented wi...
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PMID: 18585998
PDF is available here.
PDF is available here.
Journal of Biological Chemistry (283)35 2008
Abstract
We conclude that human REV1, apparently the slowest Y family polymerase, is kinetically highly tolerant to N(2)-adduct at G but not to O(6)-adducts....
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PMID: 18591245
PDF is available here.
PDF is available here.
Biochemistry (Washington) (47)33 2008
Abstract
We use selective 2'-hydroxyl acylation analyzed by primer extension (SHAPE) chemistry coupled with analysis of point mutants to map long-range interactions in this RNA. This analysis reveals two critical features of the free RNA state. First, the catalytic intron is separated from the flanking exons...
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PMID: 18642882
PDF is available here.
PDF is available here.
Microbiology (154)Pt 8 2008
Abstract
We analysed the interaction of GlnJ with known cellular targets such as the ammonium transporter AmtB1, the adenylyltransferase GlnE and the uridylyltransferase GlnD. Our results show that the interaction of GlnJ with cellular targets is regulated in vitro by the concentrations of manganese and 2-OG...
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PMID: 18667566
PDF is available here.
PDF is available here.
Abstract
Aptamers, a promising new class of therapeutics, are single-stranded oligonucleotides generated via an in vitro selection process that bind to and inhibit the activity of target proteins in a manner similar to therapeutic antibodies. In order to enhance the drug-like character of aptamers, oligonucl...
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PMID: 18644461
PDF is available here.
PDF is available here.
Abstract
We have identified a novel class I integron that carries the aac(6')-Im cassette and an IS26-disrupted copy of aadA1 (integron-IS26) in most IRAB outbreak strains and in a few ISAB non-outbreak control strains. Integron-IS26 is located chromosomally....
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PMID: 18565738
PDF is available here.
PDF is available here.
Journal of Virology (82)16 2008
Abstract
We use a simple mathematical model to explain the seemingly inconsistent data. We show that the dynamic interactions between CD8(+) T cells and antigen presentation lead to a situation where CD8(+) T cells are limiting during the initial response whereas antigen is limiting in the secondary response...
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PMID: 18550672
PDF is available here.
PDF is available here.
Journal of Virology (82)15 2008
Abstract
We report that the L protein produces an unusual cap structure, guanosine(5')tetraphospho(5')adenosine (GppppA), that is formed by the transfer of the 5'-monophosphorylated viral mRNA start sequence to GTP by the PRNTase activity before the removal of the gamma-phosphate from GTP by GTPase. Interest...
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PMID: 18495767
PDF is available here.
PDF is available here.
Abstract
Antibiotic resistance rates in P. aeruginosa were globally similar in general practice as in French hospitals. This first analysis of resistance mechanisms showed an unexpectedly high frequency of ESBLs and an unusual distribution of aminoglycoside modifying enzymes....
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PMID: 18467306
PDF is available here.
PDF is available here.
Abstract
These results suggest that Rev1 may be involved in facilitating mutagenic and recombinagenic responses to the failure of Pol delta. Genome stability, therefore, may reflect a dynamic relationship between primary and auxiliary DNA polymerases....
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PMID: 18711219
PDF is available here.
PDF is available here.
Antonie Van Leeuwenhoek (94)2 2008
Abstract
Chromate-hypersensitive mutants of the Pseudomonas aeruginosa PAO1 strain were isolated using transposon-insertion mutagenesis. Comparison of the nucleotide sequences of the regions interrupted in the mutants with the PAO1 genome revealed that the genes affected in three mutant strains were oprE (OR...
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PMID: 18446454
PDF is available here.
PDF is available here.
Molecular Cell (31)2 2008
Regulation of a eukaryotic gene by GTP-dependent start site selection and transcription attenuation.
Abstract
We show that RNA polymerase II (Pol II) responds to GTP concentration. When GTP is sufficient, Pol II initiates transcription of the IMPDH gene (IMD2) at TATA box-proximal "G" sites, producing attenuated transcripts. When GTP is deficient, Pol II initiates at an "A" further downstream, circumventing...
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PMID: 18657503
PDF is available here.
PDF is available here.
Acta Crystallographica Section D (D64)Pt 7 2008
Abstract
The X-ray polarization anisotropy of anomalous scattering in crystals of brominated nucleic acids and selenated proteins is shown to have significant effects on the diffraction data collected at an absorption edge. For conventionally collected single- or multi-wavelength anomalous diffraction data,...
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PMID: 18566507
PDF is available here.
PDF is available here.
Abstract
We report the analysis of the function of Dot1 in the response to alkylating damage. Unexpectedly, deletion of DOT1 results in increased resistance to the alkylating agent methyl methanesulfonate (MMS). This phenotype is independent of the dot1 silencing defect and does not result from reduced level...
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PMID: 18562671
PDF is available here.
PDF is available here.