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Welcome to the Pubget Medical Subject Headings (MESH) browser. Click on a topic or subtopic below to explore related papers. In the gold box is the most recent paper about the current MESH term, and below are 20 related papers.
Transferases (8):
Recent article
Transferases
FASEB Journal (24)11 2010
Abstract
We have probed at the single-molecule level the interaction of 1-deoxy-D-xylulose 5-phosphate synthase (DXS), which catalyzes the first step of the MEP pathway, with its two substrates, pyruvate and glyceraldehyde-3-phosphate. The data obtained in this pioneering SMFS analysis of a bisubstrate enzym...
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PMID: 20634351
PDF is available here.
PDF is available here.
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Journal of Antibiotics (63)10 2010
Abstract
We found that a herbicide, ketoclomazone, exhibited antibacterial activity against a pathogenic bacterium, Haemophilus influenzae, with an MIC value of 12.5 μg ml(-1) and that antibacterial activity was suppressed by adding 1-deoxyxylulose, a free alcohol of 1-deoxy-D-xylulose 5-phosphate (DXP...
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PMID: 20808315
PDF is available here.
PDF is available here.
Kidney International (78)10 2010
Abstract
Kidney International (67) 2010
Abstract
Kidney International (78)10 2010
Abstract
Kidney International (78)10 2010
Abstract
Kidney International (78)10 2010
Abstract
Glycobiology (20)7 2010
Abstract
We wondered how Giardia synthesizes dolichol phosphate (Dol-P), which is used to make N-glycans and glycosylphosphatidylinositol (GPI) anchors. Here we show that cultured Giardia makes an unsaturated polyprenyl pyrophosphate (dehydrodolichol), which contains 11 and 12 isoprene units and is reduced t...
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PMID: 20308470
PDF is available here.
PDF is available here.
Biomedical Papers (154)2 2010
Abstract
Phase II biotransformation reactions (also 'conjugation reactions') generally serve as a detoxifying step in drug metabolism. Phase II drug metabolising enzymes are mainly transferases. This review covers the major phase II enzymes: UDP-glucuronosyltransferases, sulfotransferases, N-acetyltransferas...
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PMID: 20668491
PDF is available here.
PDF is available here.
Plant Cell Reports (29)6 2010
Abstract
Plants, and specially species adapted in non-friendly environments, produce secondary metabolites that help them to cope with biotic or abiotic stresses. These metabolites could be of great pharmaceutical interest because several of those show cytotoxic, antibacterial or antioxidant activities. Leav...
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PMID: 20364257
PDF is available here.
PDF is available here.
Phytochemistry (71)8-9 2010
Abstract
We developed a sensitive assay suitable for plant extracts that is based on the decarboxylation of labeled pyruvate (1-(13)C)-PYR and detection of (13)CO(2) by isotope ratio mass spectrometry. We tested our method investigating the DXS activity in poplar leaves. Apparent DXS activity showed Michaeli...
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PMID: 20303132
PDF is available here.
PDF is available here.
médecine/sciences (26)2 2010
Abstract
Abstract
We show here that the FA content in the cell walls and the transcript levels of rice genes Os05g08640, Os06g39470, Os01g09010 and Os06g39390, are both higher in the stems than in the leaves. In addition, an RNA interference (RNAi) construct that simultaneously down-regulates transcript levels of the...
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PMID: 20012086
PDF is available here.
PDF is available here.
Abstract
We rationally modified this enzyme at the [+1]/[+2] subsites so that oNPGal would bind less tightly. Molecular modeling (MM) suggested the mutation A221W, which decreased the affinity of the donor at these sites and moved it away from the bound galactose at the -1 subsite. A single (A221W) and a dou...
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PMID: 19926593
PDF is available here.
PDF is available here.
Abstract
We searched for its protein partners using a two-hybrid screen, and identified five interacting proteins, among them Yta7p. Subsequently, we showed that Yta7p was a membrane-associated protein localized both to the nucleus and to the endoplasmic reticulum. Deletion of YTA7 affected the enzymatic act...
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PMID: 19416104
PDF is available here.
PDF is available here.
Genes & Development (23)9 2009
Abstract
We demonstrate that yeast mitochondrial glutaminyl-tRNA is in fact generated by a transamidation pathway involving a novel type of trimeric tRNA-dependent amidotransferase (AdT). More surprising is the fact that cytosolic glutamyl-tRNA synthetase (ERS) is imported into mitochondria, where it constit...
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PMID: 19417106
PDF is available here.
PDF is available here.
Abstract
1-Deoxy-D-xylulose-5-phosphate synthase (DXS) catalyses the first committed step of the 2C-methyl-D-erythritol-4-phosphate (MEP) pathway, which is an alternative isoprenoids biosynthetic route that has been recently discovered. In this work, a DXS1-like cDNA (GmDXS1) was isolated from soybean. The f...
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PMID: 18437529
PDF is available here.
PDF is available here.
Abstract
We report the first in silico analysis of grapevine DXS genes based on the whole genome sequence. Further research on the functional significance of these associations might help to understand the genetic control of Muscat flavor....
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PMID: 19037624
PDF is available here.
PDF is available here.
Abstract
Our results suggest that there is allelic and phenotypic heterogeneity of GSD III in Egypt. This is the second description of a large deletion in AGL. p.L620P is the second mutation found in GSD IIId....
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PMID: 19754354
PDF is available here.
PDF is available here.
Abstract
We aimed to assess the effects of traditional Chinese medicine; marine (MT) and kuhuang (KH), either alone or in combination, on the early graft function of the recipients and overall patient survival rate after liver transplantation (LT) by using diammonium glycyrrhizinate (DG) as a positive contro...
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PMID: 19222109
PDF is available here.
PDF is available here.
Abstract
We achieved non-ribosomal N-terminal-specific introduction of various kinds of nonnatural amino acids to a protein. A nonnatural amino acid is once charged onto an E. coli tRNA(Phe) by a mutant ARS in situ, and successively transferred from the tRNA to a target protein, namely the NEXT-A reaction. B...
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PMID: 19749248
PDF is available here.
PDF is available here.
Infection and Immunity (76)12 2008
Abstract
These results indicate that modification of LOS with PEA by LptA enhances meningococcal adhesion to human endothelial and epithelial cells in unencapsulated N. meningitidis....
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PMID: 18824535
PDF is available here.
PDF is available here.
Abstract
We cloned the novel short-chain cis-prenyltransferases from three different bacteria, and characterized their enzymatic activities to compare and elucidate a common feature of the short-chain cis-prenyltransferases. Furthermore, we identified a specific isoleucine that is conserved in short-chain ci...
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PMID: 18725204
PDF is available here.
PDF is available here.
Journal of Bacteriology (190)21 2008
Abstract
We identify a new FtsH substrate--3-deoxy-D-manno-octulosonate (KDO) transferase--which carries out the attachment of two KDO residues to the lipid A precursor (lipid IVA) to form the minimal essential structure of the lipopolysaccharide (LPS) (KDO2-lipid A). Thus, FtsH regulates the concentration o...
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PMID: 18776015
PDF is available here.
PDF is available here.
Molecules and Cells (26)3 2008
Abstract
An open reading frame, designated GerGTII and located downstream of the polyketide synthase genes, has been identified as a chalcosyltransferase by sequence analysis in the dihydrochalcomycin biosynthetic gene cluster of Streptomyces sp. KCTC 0041BP. The deduced product of gerGTII is similar to seve...
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PMID: 18612242
PDF is available here.
PDF is available here.
Plant Journal (56)1 2008
Abstract
The first step of the plastidial methylerythritol phosphate (MEP) pathway is catalyzed by two isoforms of 1-deoxy-D-xylulose 5-phosphate synthase (DXS1 and DXS2). In Medicago truncatula, MtDXS1 and MtDXS2 genes exhibit completely different expression patterns. Most prominently, colonization by arbus...
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PMID: 18557838
PDF is available here.
PDF is available here.
Abstract
We report on a target-based approach to identify possible Mycobacterium tuberculosis DXS inhibitors from the structure of a known transketolase inhibitor. A small focused library of analogs was assembled in order to begin elucidating some meaningful structure-activity relationships of 3-(4-chloro-ph...
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PMID: 18783951
PDF is available here.
PDF is available here.
Phytochemistry (69)14 2008
Abstract
Natural rubber, cis-1,4-polyisoprene, is a vital industrial material synthesized by plants via a side branch of the isoprenoid pathway by the enzyme rubber transferase. While the specific structure of this enzyme is not yet defined, based on activity it is probably a cis-prenyl transferase. Photoact...
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PMID: 18799172
PDF is available here.
PDF is available here.
Abstract
Juvenile turbot (Scophthalmus maximus) were exposed to different concentrations of the Prestige fuel oil through the diet. The effects on hepatic biotransformation enzymes, namely, 7-ethoxyresorufin O-deethylase (EROD), catalase, and phase II activities - UDP-glucuronosyltransferases (UGT) and cytos...
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PMID: 18625515
PDF is available here.
PDF is available here.
Abstract
These results support the proposed sequence of reactions for C13-O-side chain assembly in Taxol biosynthesis, and suggest that other N-transferases are responsible for the formation of related, late pathway, N-acylated taxoids....
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PMID: 18621016
PDF is available here.
PDF is available here.
Pediatric Research (64)3 2008
Abstract
We have established a transgenic mouse line (low-GCS) with reduced GCS activity (29% of wild-type (WT) C57BL/6) and accumulation of glycine in the brain (Stroke, 2007; 38:2157). The purpose of the present study is to characterize behavioral features of the low-GCS mouse as a model of mild GE. Two ot...
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PMID: 18414141
PDF is available here.
PDF is available here.
Biochemistry (Washington) (47)34 2008
Abstract
Our data show that the D98 residue that is invariant in the whole superfamily should be involved in the deprotonation of the lipid substrate during the catalytic process....
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PMID: 18672909
PDF is available here.
PDF is available here.
Biochemistry (Washington) (47)33 2008
Abstract
We have explored the promiscuous thymidylyltransferase from Streptococcus pneumoniae, Cps2L, and enhanced its uridylyltransferase and guanidyltransferase activities by active site engineering. Mutagenesis at position Q24 resulted in a variant with 10-, 3-, and 2-fold enhancement of UDP-glucosamine,...
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PMID: 18656961
PDF is available here.
PDF is available here.
Journal of Cell Biology (182)3 2008
Abstract
We show that Cosmc is an endoplasmic reticulum (ER)-localized adenosine triphosphate binding chaperone that binds directly to human T-synthase. Cosmc prevents the aggregation and ubiquitin-mediated degradation of the T-synthase. These results demonstrate that Cosmc is a molecular chaperone in the ER...
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PMID: 18695044
PDF is available here.
PDF is available here.
Abstract
We have previously used phage display methods to select a 12 residue fragment that retains recognition for modification by the Escherichia coli phosphopantetheinyltransferase (PPTase) AcpS. In this work, we have used (15)N-HSQC based NMR titration experiments of a 12-residue peptide substrate with A...
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PMID: 18593165
PDF is available here.
PDF is available here.
Abstract
Our synthetic route provided a range of key analogues with partial structures to define the pharmacophore. Simplification of the caprazamycins was further pursued to develop diketopiperazine analogs....
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PMID: 18670104
PDF is available here.
PDF is available here.
Journal of Immunology (181)3 2008
Abstract
We demonstrate that lipidation of prelipoproteins in Listeria monocytogenes is required to promote NF-kappaB activation via TLR2. In HeLa cells transiently expressing TLR2, L. monocytogenes and Listeria innocua mutants lacking the prolipoprotein diacylglyceryl transferase (lgt) gene are unable to in...
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PMID: 18641340
PDF is available here.
PDF is available here.
YAKUGAKU ZASSHI (128)7 2008
Abstract
We purified Sec synthesizing enzymes, such as seryl-tRNA synthetase (SerRS), Sec synthetase (SecS) and selenophosphate synthetase (SePS). I described the procedures to prepare Sec tRNA, SerRS, SecS, SePS and [(75)Se]H(2)Se in detail. We clarified that SecS composed of two proteins, SecSalpha and Sec...
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PMID: 18591866
PDF is available here.
PDF is available here.
DNA Sequence (19)3 2008
Abstract
I of plant DXS proteins, and HbDXS2 encodes a protein predicted to contain 711 amino acids and with a high homology to the plant DXS class II proteins. Several important motifs and amino acid positions characteristic of DXS proteins are strictly conserved in both new HbDXS proteins. The two HbDXS ge...
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PMID: 17852343
PDF is available here.
PDF is available here.
Abstract
We assessed the basal mRNA expression of 64 drug disposition genes in Fa2N-4 cells. Significant differences were found in the expression of major drug-metabolizing enzymes, nuclear receptors, and transporters between both cell types. Importantly, the expression of constitutive androstane receptor (C...
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PMID: 18332078
PDF is available here.
PDF is available here.
Abstract
Helicases and nucleic acid translocases are motor proteins that have essential roles in nearly all aspects of nucleic acid metabolism, ranging from DNA replication to chromatin remodelling. Fuelled by the binding and hydrolysis of nucleoside triphosphates, helicases move along nucleic acid filaments...
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PMID: 18414490
PDF is available here.
PDF is available here.
Abstract
Genes encoding 1-deoxy-D-xylulose 5-phosphate synthase (DXS; EC 2.2.1.7) and 2C-methyl-D-erythritol 4-phosphate synthase (MEPS; EC 1.1.1.267), the first two enzymes in the deoxyxylulose phosphate (DXP) pathway, were cloned from young leaves of Croton stellatopilosus, and designated as 1-deoxy-D-xylu...
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PMID: 18451506
PDF is available here.
PDF is available here.
Abstract
Since most studies were based on small sample sizes, replication of the findings is mandatory. Recently a large twin study confirmed a genetic predisposition to gallstones and a genome-wide association scan identified the hepatocanalicular cholesterol transporter ABCG8 as the common susceptibility f...
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PMID: 18408466
PDF is available here.
PDF is available here.
Journal of Immunology (180)9 2008
Abstract
We found that GBS strains of the same serotype differ in released factors that activate TLR2. Several lines of genetic and biochemical evidence indicated that lipoteichoic acid (LTA), the most widely studied TLR2 agonist in Gram-positive bacteria, was not essential for TLR2 activation. We thus exami...
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PMID: 18424736
PDF is available here.
PDF is available here.
Abstract
An extracellular enzyme (RMEBE) possessing alpha- (1-->4)-(1-->6)-transferring activity was purified to homogeneity from Rhodothermus marinus by combination of ammonium sulfate precipitation, Q-Sepharose ion-exchange, and Superdex- 200 gel filtration chromatographies, and preparative native polyacry...
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PMID: 18388462
PDF is available here.
PDF is available here.
Abstract
The biosynthesis of bacterial cell wall peptidoglycan is a complex process involving many different steps taking place in the cytoplasm (synthesis of the nucleotide precursors) and on the inner and outer sides of the cytoplasmic membrane (assembly and polymerization of the disaccharide-peptide monom...
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PMID: 18081839
PDF is available here.
PDF is available here.
Nucleic Acids Research (36)4 2008
Abstract
We present the crystal structure of Methanococcus maripaludis SepSecS complexed with PLP at 2.5 A resolution. SepSecS, a member of the Fold Type I PLP enzyme family, forms an (alpha2)2 homotetramer through its N-terminal extension. The active site lies on the dimer interface with each monomer contri...
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PMID: 18158303
PDF is available here.
PDF is available here.
Abstract
We show that 3CLpro has mechanistic features common and disparate to the archetypical proteases papain and chymotrypsin. The kinetic mechanism for 3CLpro-mediated ester hydrolysis, including the individual rate constants, is consistent with a simple double displacement mechanism. The pre-steady-stat...
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PMID: 18237196
PDF is available here.
PDF is available here.
Abstract
These results indicated that avidity played a key role in the inhibition of cell proliferation by these antibodies against the human EGFR, suggesting that their mechanisms of action are similar....
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PMID: 18232715
PDF is available here.
PDF is available here.
Abstract
We have delineated a 450-residue core of mouse SecS, which retained full selenocysteyl-tRNA [Ser]Sec synthesis activity, and determined its crystal structure at 1.65 A resolution. SecS exhibits three domains that place it in the fold type I family of pyridoxal phosphate (PLP)-dependent enzymes. Two...
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PMID: 18093968
PDF is available here.
PDF is available here.
Abstract
The production of epothilone mixtures is a direct consequence of the substrate tolerance of the module 3 acyltransferase (AT) domain of the epothilone polyketide synthase (PKS) which utilises both malonyl- and methylmalonyl-CoA extender units. Particular amino acid motifs in the active site of AT do...
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PMID: 18219420
PDF is available here.
PDF is available here.
Microbiology (154)Pt 2 2008
Abstract
We investigated the contribution of each region to WbaP function by constructing a series of mutant WbaP proteins and using them to complement O antigen synthesis in DeltawbaP mutants of S. enterica serovars Typhi and Typhimurium. Truncated forms of WbaP lacking the periplasmic loop exhibited altere...
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PMID: 18227248
PDF is available here.
PDF is available here.
Abstract
Real-time polymerase chain reaction (PCR)-based methodology for the determination of rRNA gene (rrn) copy number was introduced and demonstrated. Both absolute and relative quantifications were tested with Escherichia coli. The separate detection of rRNA gene and chromosomal DNA was achieved using t...
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PMID: 18074129
PDF is available here.
PDF is available here.
Infection and Immunity (76)2 2008
Abstract
We found several strains in which there were two distinct but variant lic1D DNA sequences, genes predicted to encode the transferase responsible for directing the addition of PCho to LPS. The same isolates were also found to express concomitantly two PCho residues at distinct positions in their LPS....
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PMID: 17984210
PDF is available here.
PDF is available here.
Abstract
Two gem-difluoromethylenated nucleoside moieties of liposidomycins, 3 and 4, were designed and synthesized. Compound 3 was assembled from lactol 5 and gem-difluoromethylenated nucleoside 6. In the synthesis of target molecule 4, the coupling of the trichloroacetimidate derivative of gem-difluorometh...
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PMID: 18075661
PDF is available here.
PDF is available here.
Abstract
Abstract
Experimental thyrotoxicosis in rats is accompanied by the increase of serum alanine aminotransferase (AlA), aspartate aminotransferase (AsA), creatine kinase-MB (CK-MB) activities and content of primary products of lipid peroxidation--conjugated dienes--in liver, heart and blood. Thi...
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PMID: 18421916
PDF is available here.
PDF is available here.
The associations between height components (leg and trunk length) and adult levels of liver enzymes.
Abstract
Adult liver function is affected by early life environmental exposures as reflected in leg length, and this may suggest common childhood influences on liver development and adult risk of diabetes and coronary heart disease. Further studies with detailed measures of early life exposures relevant to l...
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PMID: 18079333
PDF is available here.
PDF is available here.
Drug Metabolism Reviews (40)2 2008
Abstract
Our current understanding of regulatory polymorphisms impacting drug disposition is reviewed including specific discussion regarding knowledge gaps and future research opportunities....
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PMID: 18464046
PDF is available here.
PDF is available here.
Pediatrics International (49)6 2007
Abstract
The mean serum SCF level of the BA children was higher than that of normal controls (748.3 +/- 17.9 pg/mL vs 582.2 +/- 17.3 pg/mL; P < 0.001). Subsequent analysis demonstrated that the BA patients with serum ALT > or = 100 U/L had significantly greater levels of serum SCF compared to those with seru...
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PMID: 18045291
PDF is available here.
PDF is available here.