Abstract
An antihypertensive peptide, Lys-Val-Leu-Pro-Val-Pro (KVLPVP), can reduce blood pressure in hypertensive rats after being orally administered. In this study, the transepithelial transport of intact KVLPVP was examined by Caco-2 monolayers. The results were as follows: (i) The flux was not saturable...
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PMID: 19202266
PDF is available here.
Abstract
The purpose of this investigation was to study the effect of Streptomyces erumpens cells immobilized in various matrices, i.e., agar-agar, polyacrylamide, and luffa (Luffa cylindrica L.) sponge for production of alpha-amylase. Luffa sponge was found to be 21% and 51% more effective in enzyme yield t...
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PMID: 18521760
PDF is available here.
Abstract
A high-throughput and sensitive liquid chromatography/tandem mass spectrometry assay was established to detect total unlabeled hepatitis C virus inhibitor concentrations in replicon cells. The intracellular concentrations determined by this assay correlated well with concentrations obtained using ra...
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PMID: 18790690
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Abstract
Vertical and horizontal spatial variability in the biodegradation of the herbicide bentazone was compared in sandy-loam soil from an agricultural field using sieved soil and intact soil cores. An initial experiment compared degradation at five depths between 0 and 80 cm using sieved soil. Degradatio...
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PMID: 18768205
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Abstract
These results suggest that late incubation conditions affect long bone development in broilers....
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PMID: 19093239
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Abstract
1. Changes in water loss, eggshell conductance and hatchability with flock age were monitored in layer hens in a commercial hatchery. 2. Optimal water loss for maximal hatchability of layer eggs was found to be 12 to 13% of initial egg mass at d 18 of incubation. 3. Mass specific water vapour conduc...
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PMID: 19093240
PDF is available here.
Abstract
We have examined the self-assembled membrane-bound aggregates of two annexin V (A5) dye conjugates and compared them to those from native A5. Native A5 and FITC-labeled A5 (A5-FITC) both formed discrete well-defined crystalline monolayer domains of p6 symmetry. However, A5-FITC also showed additiona...
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PMID: 18783196
PDF is available here.
Abstract
We report a novel Dual Approach to Random Chemical Mutagenesis (DuARCheM) to introduce random mutations in a defined DNA fragment. DuARCheM involves in vivo chemical mutagenesis and in vitro genetic manipulations. The resulting library revealed an accumulation of mutations in its members. These resu...
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PMID: 18756549
PDF is available here.
Abstract
Two experiments, which included 3 incubators, were carried out to investigate the effects of egg weight and position relative to incubator (setter) fan on embryonic mortality, second quality chicks, and fertile hatchability of broiler eggs. Three egg weight groups termed small (approximately 62.4 g)...
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PMID: 18753462
PDF is available here.
Abstract
We use microfluidic devices to encapsulate, incubate, and manipulate individual cells in picoliter aqueous drops in a carrier fluid at rates of up to several hundred Hz. We use a modular approach with individual devices for each function, thereby significantly increasing the robustness of our system...
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PMID: 18584086
PDF is available here.
Abstract
Formaldehyde cross-linking of proteins is emerging as a novel approach to study protein-protein interactions in living cells. It has been shown to be compatible with standard techniques used in functional proteomics such as affinity-based protein enrichment, enzymatic digestion, and mass spectrometr...
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PMID: 18513538
PDF is available here.
Abstract
We recently discovered new nucleotides (4-pyridone-3-carboxamide-1-beta -D-ribonucleoside phosphates) in human erythrocytes. To establish the precursor compound and pathways of nucleotide derivative formation and breakdown, human erythrocytes were incubated for 3 hours with 0.3 mM 4-pyridone-3-carbo...
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PMID: 18600548
PDF is available here.
Abstract
A universal platform of homogeneous noncompetitive immunoassay, using human immunoglobulin (IgG) as a model analyte, has been developed. The assay is based on aggregation of antibody-functionalized gold nanoparticles directed by the immunoreaction coupled with light scattering detection with a commo...
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PMID: 18585169
PDF is available here.
Abstract
The aim of the research was to evaluate embryo composition and changes in egg yolk fatty acid composition during embryonic development as a function of incubation temperature and age of breeders. Eggs obtained from a common breeder stock at 3 ages: 32 (younger), 42 (mid age), and 65 (older) wk were...
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PMID: 18493015
PDF is available here.
Abstract
Inoculation with Azospirillum brasilense Sp245 exerts beneficial effects on micropropagated plants of Prunus cerasifera L. clone Mr.S 2/5, as seen in the results of a comparative analysis of inoculated and non-inoculated explants, during both the rooting and acclimatation phases. The presence of Azo...
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PMID: 18358553
PDF is available here.
Abstract
The biochemical routes for the metabolism of methylglyoxal and the formation of lactaldehyde and hydroxyacetone in Methanocaldococcus jannaschii have been established. The addition of methylglyoxal and NADH, NADPH, F 420H 2, or DTT to a M. jannaschii cell extract stimulated the production of both la...
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PMID: 18363381
PDF is available here.
Abstract
OBJECTIVE: To perform a quantitative and qualitative analysis of the environmental microorganisms associated with two assisted reproductive technology (ART) laboratories. DESIGN: Prospective descriptive study. SETTING: Two ART laboratories at Greenville Hospital System (GHS) University Medical Cente...
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PMID: 17678906
PDF is available here.
Abstract
A small-scale, economical isolator system was adapted to hatch and raise chicks in a bacteria-free environment as a means to observe bacterial interactions with the intestinal mucosa during early development. The design and construction of flexible plastic isolators for incubation and brooding are d...
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PMID: 18435876
PDF is available here.
Abstract
We review the general topic of incubator management as it pertains to ART. Discussed within the context of this article will be our experiences as they relate to incubator management. Details as they apply to incubator environment also will include gamete/embryo positions within incubator, air quali...
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PMID: 17524397
PDF is available here.
Abstract
Valdecoxib is a new anti-inflammatory drug that is highly selective for inhibition of the inducible form of cyclooxygenase (COX-2). In the present study, biotransformation of valdecoxib was investigated in cell cultures of five medicinal plants, viz., Catharanthus roseus, Azadirachta indica, Capsicu...
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PMID: 18556810
PDF is available here.
Abstract
Despite thorough selection during the last decade, the incidence of ascites is still high in modern broiler strains. Although ascites occurs mostly at the end of the rearing period, there are indications that the etiology of this problem may have started during embryonic development. Recent studies...
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PMID: 18281584
PDF is available here.
Abstract
During studies directed towards the discovery of nitrile hydrolysing enzymes from thermophiles, vanillin aldoxime was incubated with the thermophilic organism, Geobacillus sp. DDS012 isolated from Italian rye grass (Lolium multiflorum) silage. The predominant product was a dihydro-dimer, which could...
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PMID: 18264580
PDF is available here.
Abstract
Lactobacillus plantarum strains IS-10506 and IS-20506 isolated from Indonesian traditional fermented milk, dadih, were screened for their ability to remove the cyanobacterial toxin microcystin-LR (MC-LR) from aqueous solution (100 microg/L) at 22 and 37 degrees C. The objective was to study the main...
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PMID: 17928120
PDF is available here.
Abstract
A bacterium classified as Achromobacter xylosoxidans strain IR08 by phenotypic typing coupled with 16S rRNA gene analysis was isolated from a soil contaminated with electrical transformer fluid for over sixty years using Aroclor 1221 as an enrichment substrate. The substrate utilization profiles rev...
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PMID: 19143351
PDF is available here.
Abstract
We aimed to verify whether incubation at 37 degrees C could affect the morphologic integrity of sperm nuclei. DESIGN: Time-kinetics studies testing fine morphology of the sperm nuclei upon prolonged in vitro incubation. SETTING: Male Fertility Laboratory at Bar-Ilan University, Ramat Gan, Israel. PA...
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PMID: 17481619
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Abstract
The ostrich industry experiences a high rate of embryonic mortalities during artificial incubation of eggs. Embryonic deaths were studied from data recorded on 37,740 fertile eggs incubated artificially during the 1998-2005 breeding seasons. Roughly 10,000 eggs that sustained embryonic mortalities w...
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PMID: 18507217
PDF is available here.
Abstract
Phototrophic biofilms are defined as interfacial microbial communities mainly driven by light as energy source and are studied for both ecological and technological reasons. Field investigations of biofilms usually do not offer the opportunity to study the effects of a large number of external param...
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PMID: 17590463
PDF is available here.
Abstract
Our results also suggest that some stages of development are more sensitive to dexamethasone administration as effects can be influenced by early incubation protocols....
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PMID: 16697137
PDF is available here.
Abstract
Many environmental conditions for in vitro embryo production (IVP) systems for cattle have been relatively standardised, e.g. media composition, temperature, pH, water quality, and atmospheric composition. However, little attention has been paid to the quality of ambient laboratory air and the gas e...
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PMID: 17331571
PDF is available here.
Abstract
Temperature recovery after a 5-s door opening/closing procedure was approximately 5 min for the mini-incubator and 30 min for the conventional incubator. The oxygen concentration return was significantly improved in the mini-incubator (3.0 +/- 0 min) compared with the conventional incubator (7.8 +/-...
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PMID: 17160731
PDF is available here.
Abstract
This study investigated the effect of non-ventilation of the incubator during the first 10 days of incubation on carbon dioxide (CO(2)) concentrations in the incubator and its effects on the embryonic and post-hatch development of the chicken (Gallus gallus). Two different incubation conditions were...
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PMID: 16928458
PDF is available here.
Abstract
A novel solid-state fermentation apparatus, namely an upright multi-tray conidiation chamber, was developed to facilitate the production of aerial conidia of fungal biocontrol agents, such as Beauveria bassiana. The chamber with 25 bottom-meshed metal trays had a capacity of > or =50 kg rice with ea...
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PMID: 16786244
PDF is available here.
Abstract
Two experiments were carried out to study embryonic metabolic responses to short-term temperature fluctuations in order to explore the possibilities of using embryonic metabolic responses as a tool to control the incubation process. In the first experiment, eggshell temperature (ET) in the control g...
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PMID: 16776478
PDF is available here.
Abstract
An experiment was conducted to study the effect of egg size on embryo development, heat production, and energy partitioning between egg and hatchling. Small (56.1 +/- 0.12 g SEM) and large (70.0 +/- 0.11 g SEM) hatching eggs were incubated in climate respiration chambers, and eggshell temperature wa...
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PMID: 16615362
PDF is available here.
Abstract
We investigated the effects of HEPES in the medium (to maintain pH) and paraffin oil covering the medium (to maintain osmolality) on the developmental ability of porcine embryos produced in vitro using tightly closed glass tubes in the absence of a CO2 gas-regulated incubator. Putative porcine zygot...
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PMID: 16084577
PDF is available here.
Abstract
We here describe how to produce, from commercially available plastic wide-mouth jars, very simple gas-tight containers that can be flushed with prepared gas mixtures to produce low-oxygen environments for standard cell culture. This permits any laboratory to easily try the effects of physiological o...
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PMID: 17487199
PDF is available here.
Abstract
The existing toxicity data on the effects of polycyclic aromatic hydrocarbons (PAHs) on Pseudokirchneriella subcapitata (green alga) are quite insufficient. These data were derived using different test techniques (e.g. conventional batch test, closed-system test, semi-static test). The relative toxi...
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PMID: 17302334
PDF is available here.
Abstract
Temperature increase was most marked in the foil-covered hood followed by cloth-covered hood, both with the heater and the volunteer. Similarly, in the volunteer study, the humidity was best maintained inside the aluminium foil-covered hood. CONCLUSION: We recommend the use of regular hood with suit...
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PMID: 17050931
PDF is available here.
Abstract
The temperature variations within the chambers were <0.3 degrees C, and the variation between the chambers was on average <0.05 degrees C. After eight hours the pH values of the cell culture media were 7.47 and 7.48 in the reference and measurement chambers, respectively; 7.41 in the CO(2) incubator...
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PMID: 16315215
PDF is available here.
Abstract
Nebulization of aerosolized medications in infants with evolving BPD by hood was less time-consuming for caregivers and was much better tolerated by the infants while being at least as effective as the conventional facemask nebulization....
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PMID: 16341026
PDF is available here.
Abstract
We studied the affects of storage period and egg weight on the hatchability of 314 ostrich (Struthio camelus) eggs. Eggs were stored at 20 degrees C and 65% RH before incubation at the Poultry Research, Teaching and Extension Center at Texas A&M University (College Station, TX). Eggs were classed by...
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PMID: 16479949
PDF is available here.
Abstract
Contamination and penetration of salmonellae into hatching eggs may comprise an important link in the transmission of these bacteria to growing birds, processed carcasses, and eventually to the consumer. In this study, a predictive model for Salmonella typhimurium as a function of initial cell numbe...
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PMID: 16479956
PDF is available here.
Abstract
The cereal-grain WPs, both mung beans and dry corn, were equally effective in producing heat when warmed in the microwave oven. The mean maximal temperatures at the surface of brown envelope were too high for direct application to newborns. The mean maximal temperatures of towels covering the paper...
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PMID: 16856441
PDF is available here.
Abstract
The effects of asymmetrical hatching behaviors on the development of turning bias and footedness in domestic chicks, bobwhite quail, and Japanese quail chicks were examined. Control tests with incubator reared domestic chicks and bobwhite quail revealed significant individual and population left-sid...
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PMID: 16136548
PDF is available here.
Abstract
We used callus of Echinacea augustifolia for isolation and researched the factors influencing the process of protoplasts preparation, the result indicated it was easy to isolate protoplast from buff-green Callus in E. augustifolia which was looked like granule with symmetrical character. The result...
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PMID: 15979859
PDF is available here.
Abstract
In this manuscript, a new tool is presented for automatic monitoring of the hatching process of eggs without opening the incubator doors. The amount of energy in the noise produced by the hatchlings was registered by means of a microphone and a personal computer for signal treatment. Evidence is sho...
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PMID: 16050127
PDF is available here.
Abstract
The need to transport oocytes and embryos between two laboratories have prompted us to evaluate the effects of in vitro maturation of immature mouse oocytes in a CO2-deficient dry heat portable incubator and subsequent in vitro development of these fertilized mouse oocytes in a standard CO2 incubato...
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PMID: 15826691
PDF is available here.
Abstract
We showed that describing an egg as a sphere underestimated convective heat transfer by 33% and was, therefore, too simplistic to accurately assess actual heat transfer from real eggs....
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PMID: 15782902
PDF is available here.
Abstract
The present study was conducted to investigate whether a portable CO2 incubator was effective for in vitro maturation (IVM) of bovine, porcine and minke whale oocytes, and the effect of maturation media supplemented with different hormones; porcine follicle stimulating hormone (pFSH), estradiol-17be...
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PMID: 15750298
PDF is available here.
Abstract
We concluded that egg turning was required during incubation until d 12, at least, and should not be stopped until after d 15....
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PMID: 15742969
PDF is available here.
Abstract
The detection of a positive by either the Steritest compact system or the BacT/ALERT system was summarized for each organism in each validation study. Data generated from studies reducing the incubation temperature from 35 degrees C to 32 degrees C improved detection times in the automated method co...
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PMID: 16306015
PDF is available here.
Abstract
The amount of light reduction provided by incubator covers varies depending on type of fabric as well as percentage of incubator surface shielded by the cover. Dark-colored covers provided greater light reduction than bright/light-colored covers when covers identical in fabric type were compared. Th...
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PMID: 15835478
PDF is available here.
Abstract
We measured EMF at mattress level in three neonatal incubators with and without ferromagnetic panels between the electric motor and the mattress. We then measured the EMF at the level of the maximum emission point for caregivers, i.e., near the display panel. The ferromagnetic panels were (a) 5 mm t...
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PMID: 15719965
PDF is available here.
Abstract
Variability of egg weight, egg yolk content, neonatal growth rate and relationships of these parameters were studied in meat-type chicks. As it had been established the level of variability in neonatal growth traits was greater than variability of the egg morphology parameters. Egg weight had strong...
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PMID: 15859454
PDF is available here.