Abstract
We selected bacteria that potentially could be passed through 0.22- or 0.45-microm-pore-size filters and then PCR amplified the 16S rDNA genes using the universal primer pairs 27F/1492R and 63F/1387R. The PCR-amplified rDNAs extracted from bacteria that had been passed through 0.45-microm-pore-size...
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PMID: 20513960
PDF is available here.
Abstract
The second part of the article presents the results of a study of antimicrobial activity of filter nonwovens with an addition of biocides, as a function of the presence of sweat in the environment and the method of microbe deposition on a nonwoven in the form of a liquid and a bioaerosol. At the sam...
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PMID: 20540845
PDF is available here.
Abstract
This paper presents the results of a study on antimicrobial activity of polymer filter nonwovens produced by needle-punching or melt-blowing with an addition of disinfecting agents. The first part of the paper discusses how the biocidal activity of nonwovens is a function of the active agent added t...
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PMID: 20540844
PDF is available here.
Abstract
Haemofilter TMP remained unchanged in G1 while it increased up to three times in G2 (P=0.0002). The median filter lifespan was 68 h in G1 and 19 h in G2. The rate of spontaneous circuit failure was 24% in G1 and 93% in G2 (P=0.0001). The platelet count was stable over the treatment period in G1 whil...
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PMID: 19650808
PDF is available here.
Abstract
77.2% of all GIBs were MF-mounted. 33.1% of mounted GIBs versus 48.8% of non-mounted GIBs were assessed as fully acceptable sections. The differences between these figures are not statistically significant. 41.7% of the mounted GIBs were placed with the mucosal surface facing the MF, which entails a...
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PMID: 19758509
PDF is available here.
Abstract
The membrane concentration method recovered >80% of Campylobacter phages from spiked samples. The absence of Campylobacter phages in environmental samples, from both enrichment and concentration methods, suggests that, if present, they are at very low titres. SIGNIFICANCE AND IMPACT OF THE STUDY: Te...
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PMID: 19187501
PDF is available here.
Parasitology (88 ( Pt 1))01 2009
Abstract
We have performed restriction endonuclease digestion of nuclear DNAs, combined with gel electrophoresis and molecular hybridization to characterize different Trypanosoma brucei brucei stocks and to identify trypanosome species. Cloned DNA complementary to the messenger RNAs (mRNAs) for different var...
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PMID: 6324058
PDF is available here.
Abstract
A simplified method for the purification of cholera toxin was developed. The 569B strain of Vibrio cholerae, a recognized hyper-producer of cholera toxin, was propagated in a bioreactor under conditions that promote the production of the toxin. The toxin was separated from the bacterial cells using...
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PMID: 19190416
PDF is available here.
Abstract
We propose the continual monitoring of the water supplies in hemodialysis centers and the adoption of effective prophylactic measures that minimize the exposure of these immunodeficient patients to contaminated sources of water....
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PMID: 19229389
PDF is available here.
Abstract
Miniaturised liquid/liquid interfaces provide benefits for bioanalytical detection with electrochemical methods. In this work, microporous silicon membranes which can be used for interface miniaturisation were characterized by simulations and experiments. The microporous membranes possessed hexagona...
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PMID: 19082187
PDF is available here.
Abstract
A quantitative reverse-phase HPLC method with UV detection, for lumefantrine (LF) and desbutyllumefantrine (DLF) in whole blood spotted on filter paper was developed. The analytes were stabilized on filter paper by treatment of blood with phosphoric acid (1.6 mol/L). Halofantrine was...
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PMID: 19026600
PDF is available here.
Abstract
We have investigated the alternative use of tangential flow filtration for preparation of membranes from Escherichia coli. This method proved to be superior to the conventional use of ultracentrifuges both in speed and in yield of membrane protein. Moreover, it could more readily be scaled up to pro...
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PMID: 19021076
PDF is available here.
Abstract
We report a case of internal carotid artery (ICA) stenosis complicated with filter obstruction during CAS. A 74-year-old woman who had a history of coronary artery stenting was referred to our hospital for the treatment of carotid artery stenosis. Angiography showed occlusion of the right ICA and hi...
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PMID: 19086445
PDF is available here.
Abstract
A bacterial filter causes a small difference but is not clinically significant, with a wide spread comparable to the variability of the technique and recommended cut-offs for assessing repeatability and bronchodilation. Age, height or severity of obstruction need not be corrected for in general.
(c)...
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PMID: 18618618
PDF is available here.
Abstract
B. atrophaeus spores at levels <10 CFU l(-1) were concentrated directly from tap water using an automated dead-end hollow-fibre ultrafiltration system. SIGNIFICANCE AND IMPACT OF THE STUDY: The prototype concentrator represents a critical step towards an autonomous system that could be installed in...
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PMID: 18298529
PDF is available here.
Abstract
The Lis-1400-aided FISHFC method is more efficient than conventional methods for enumeration of viable Listeria spp. in food samples. SIGNIFICANCE AND IMPACT OF THE STUDY: For enumeration of Listeria spp., Lis-1400-aided FISHFC method is equally accurate yet faster than conventional plate count meth...
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PMID: 18298523
PDF is available here.
Abstract
Reference values of IGF-I for African boys and girls were determined. They will be used for endocrine evaluations and nutritional monitoring....
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PMID: 18329308
PDF is available here.
M Yunoki,
S Yamamoto,
H Tanaka,
H Nishigaki,
Y Tanaka,
A Nishida,
J Adan-Kubo,
M Tsujikawa,
S Hattori,
T Urayama,
M Yoshikawa,
I Yamamoto,
K Hagiwara and
K Ikuta
Abstract
Hepatitis E virus isolates in albumin solutions were inactivated slowly at 60 degrees C for 5 h and the resultant log reduction factor (LRF) was from 1.0 to > or = 2.2, whereas the virus in PBS was inactivated quickly to below the detection limit and the LRF was > or = 2.4 to > or = 3.7. The virus i...
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PMID: 18714441
PDF is available here.
Abstract
The analytical performance characteristics of our 17-OHP Neonatal UMELISA suggest that it can be used for the neonatal screening of CAH....
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PMID: 18442476
PDF is available here.
Abstract
In tungsten refining and manufacturing processes, a series of tungsten oxides (WO(X)) are typically formed as intermediates in the production of tungsten powder. Studies in the Swedish tungsten refining and manufacturing industry have shown that intermediate tungsten refining processes can create WO...
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PMID: 18569509
PDF is available here.
Abstract
In a field survey of enteric viruses, water samples collected sometimes need to be stored for a long duration before analysis is performed. The aim of this study was to develop an appropriate sample storage method for detecting viruses in environmental water. Three types of sample storage methods we...
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PMID: 18499274
PDF is available here.
Abstract
Robust filtering techniques capable of efficiently removing particulates and biological agents from water or air suffer from plugging, poor rejuvenation, low permeance, and high backpressure. Operational characteristics of pressure-driven separations are in part controlled by the membrane pore size,...
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PMID: 17884208
PDF is available here.
Abstract
We report the first case of anaphylactoid reactions caused by a white-cell reduction filter with a positively charged surface....
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PMID: 18490198
PDF is available here.
Abstract
Novel aerobic granular sludge membrane bioreactor (GMBR) was established by combining aerobic granular sludge technology with membrane bioreactor (MBR). GMBR showed good organics removal and simultaneous nitrification and denitrification (SND) performances for synthesized wastewater. When influent t...
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PMID: 18465123
PDF is available here.
Abstract
This study demonstrated that various fungi were present in the water system. These data suggest the inclusion of the detection and quantification of fungi in the water of haemodialysis. SIGNIFICANCE AND IMPACT OF THE STUDY: The recovery of fungi from aqueous haemodialysis environments implies a pote...
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PMID: 18363650
PDF is available here.
Abstract
The presence of antibiotic- and biosurfactant-producing strains of fluorescent pseudomonads in a closed hydroponic system equipped with a slow filter was investigated. A total of 271 strains of pseudomonads were isolated before the filter, from the filter skin and from the effluent. Production of bi...
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PMID: 18058254
PDF is available here.
Abstract
We described a novel approach to directly amplify genomic DNA from whole blood and dried blood spotted on filter paper without any DNA isolation by using the PCR buffer with a higher pH, which was optimized as pH 9.1-9.6. Direct PCR on blood treated with various anticoagulants showed that the buffer...
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PMID: 18249179
PDF is available here.
Ricardo P Santos,
Theodora T P Arruda,
Cibele B M Carvalho,
Victor A Carneiro,
Lara Q V Braga,
Edson H Teixeira,
Francisco V S Arruda,
Benildo S Cavada,
Alexandre Havt,
Taianá M de Oliveira,
Gustavo A Bezerra and
Valder N Freire
Abstract
We used AFM to investigate topographical changes during the development process of Enterococcus faecalis biofilms, which were generated on sterile cellulose nitrate membrane (CNM) filters in brain heart infusion (BHI) broth agar blood plates after 24, 36, 72, 192, and 360 h. AFM height images showed...
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PMID: 18312720
PDF is available here.
Abstract
An enzyme reactor installed with ultrafiltration membrane was developed to produce alpha-, beta-, and gamma-cyclodextrins (CDs) from soluble starch by Bacillus macerans cyclodextrin glycosyltransferase (CGTase) tagged with 10 lysines at its C-terminus (CGTK10ase). Ultrafiltration membrane YM10 with...
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PMID: 18467867
PDF is available here.
Abstract
Introducing three dedicated virus-clearance steps in the manufacturing process of immunoglobulins from human plasma provides high margins of safety....
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PMID: 18167162
PDF is available here.
Abstract
The authors investigated the bactericidal activity of high-chlorine-content nanoporous carbide-derived carbon (CDC) against the Gram-positive, spore-forming bacterium Bacillus anthracis and the common Gram-negative enteric bacterium Escherichia coli. Chlorine-loaded nanoporous CDC produced by thermo...
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PMID: 17635016
PDF is available here.
Abstract
We could guarantee high stability and selectivity during all measurements carried out at 37 degrees C. Overall solvent productivity of fermentation connected with continuous product removal by pervaporation was 2.34 g l(-1) h(-1). The supported ionic liquid membrane (SILM) was impregnated with 15 wt...
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PMID: 18231789
PDF is available here.
Elvira Vaclavik Bräuner,
Lykke Forchhammer,
Peter Møller,
Lars Barregard,
Lars Gunnarsen,
Alireza Afshari,
Peter Wåhlin,
Marianne Glasius,
Lars Ove Dragsted,
Samar Basu,
Ole Raaschou-Nielsen and
Steffen Loft
Abstract
Reduction of particle exposure by filtration of recirculated indoor air for only 48 hours improved MVF in healthy elderly citizens, suggesting that this may be a feasible way of reducing the risk of cardiovascular disease....
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PMID: 17932377
PDF is available here.
Abstract
Mabs of the isotypes IgG and IgM dried on filter papers were not affected by duration of storage. The detection by serotyping Mabs was generally consistent for dried filter paper MAb samples stored frozen for over 1 year at -20 degrees C, and although decreased reactive antibody titers were found af...
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PMID: 18325118
PDF is available here.
Scott Lute,
William Riordan,
Leonard F Pease,
De-Hao Tsai,
Richard Levy,
Mohammed Haque,
Jerold Martin,
Ichiro Moroe,
Terry Sato,
Michael Morgan,
Mani Krishnan,
Jennifer Campbell,
Paul Genest,
Sherri Dolan,
Klaus Tarrach,
Anika Meyer,
PDA Virus Filter TAsk Force,
Michael R Zachariah,
Michael J Tarlov,
Mark EtzeL,
Kurt Brorson,
Hazel Aranha,
Mark Bailey,
Jean Bender,
Jeff Carter,
Qi Chen,
Chris Dowd,
Raj Jani,
David Jen,
Stanley Kidd,
Ted Meltzer,
Kathryn Remington,
Iris Rice,
Cynthia Romero,
Terry Sato,
Maik Jornitz,
Carol Marcus Sekura,
Gail Sofer,
Rachel Specht,
Peter Wojciechowski and
Parenteral Drug Association Virus Filter Task Force
Abstract
Virus filters are membrane-based devices that remove large viruses (e.g., retroviruses) and/or small viruses (e.g., parvoviruses) from products by a size exclusion mechanism. In 2002, the Parenteral Drug Association (PDA) organized the PDA Virus Filter Task Force to develop a common nomenclature and...
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PMID: 19055228
PDF is available here.
Kurt Brorson,
Scott Lute,
Mohammed Haque,
Jerold Martin,
Terry Sato,
Ichiro Moroe,
Michael Morgan,
Mani Krishnan,
Jennifer Campbell,
Paul Genest,
Joseph Parrella,
Sherri Dolan,
Susan Martin,
Klaus Tarrach,
Richard Levy,
PDA Virus Filter Task Force,
Hazel Aranha,
Mark Bailey,
Jean Bender,
Jeff Carter,
Qi Chen,
Chris Dowd,
Raj Jani,
David Jen,
Stanley Kidd,
Ted Meltzer,
Kathryn Remington,
Iris Rice,
Cynthia Romero,
Terry Sato,
Maik Jornitz,
Carol Marcus Sekura,
Gail Sofer,
Rachel Specht and
Peter Wojciechowski
Abstract
We evaluate the method and find it to be acceptable for testing scaled-down models of small virus-retentive filters from four manufacturers. Three consecutive lots of five filter types were tested (Pegasus SV4, Viresolve NFP, Planova 20N and 15N, Virosart CPV). Each passed the criteria specified in...
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PMID: 19055229
PDF is available here.
Abstract
Amongst atmospheric pollutants, two major types can be distinguished: airborne particles such as dust, and gaseous compounds such as Volatile Organic Compounds (VOCs). Fibrous filters are commonly used to remove particles while activated carbon beds trap VOCs. In order to obtain a single-stage devic...
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PMID: 18341147
PDF is available here.
Abstract
Recovery of spores using membrane filtration is dependent on the manufacturer and filter pore size. Correlations between spore recovery rate and spore size could not be determined. SIGNIFICANCE AND IMPACT OF THE STUDY: Low numbers of Alicyclobacillus spores in juice can be effectively detected using...
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PMID: 17908225
PDF is available here.
Abstract
Consisted of beef extract (3 grams), peptone (10 grams), sodium chloride (5 grams), agar (20 grams) and distilled water, the solid medium was used with filter paper for cultivation of the larvae of Ancylostoma caninum. The method shows higher detection rate and simpler operation than the traditional...
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PMID: 18038814
PDF is available here.
Abstract
Eleven 16S rRNA gene clone libraries including 34 archaeal and 72 bacterial phylotypes were constructed from total 708 clones of hydrothermal vent prokaryotes trapped by 0.2- and 0.1-microm-pore-size filters. Crenarchaeota and Proteobacteria phylotypes dominated the archaeal and bacterial population...
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PMID: 17401540
PDF is available here.
Abstract
We noticed the obstruction of the venous line, and pushed 30 ml of blood with 10 ml syringe. However, it was difficult to return her blood through the venous line. At that time, a sudden onset of red urine without any special symptom occurred. We investigated the cause of the red urine, whether this...
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PMID: 17633851
PDF is available here.
Abstract
Several protocols have been described for the detection of genomes of enteric viruses from water using two-step procedures: membrane filtration and RT-PCR detection. However, these methods, when applied to bottled water, generally consider only the aqueous phase. Such procedures do not take into acc...
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PMID: 17374404
PDF is available here.
Abstract
The recovery efficiency of naked poliovirus-RNA in water using virus concentration methods was determined to evaluate the possibility of detecting naked viral genomes. Two conventional virus concentration methods (1MDS-method and HA-method) and two methods developed by our research group (Mg-method...
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PMID: 17321606
PDF is available here.
Abstract
We present a routine protocol for FISH in soil including the use of Nycodenz. The protocol allows fast and easy enumeration of hundreds of bacteria. We propose the use of silicon grease coated slides to treat in parallel seven samples per hybridization. Further, we developed a semi-automated approac...
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PMID: 17442439
PDF is available here.
Abstract
Recombinant baculoviruses carrying mammalian expression cassettes or "BacMam" are promising gene delivery vehicles shown to transduce mammalian cells efficiently both in vitro and in vivo. These viruses are vectors of choice because they are non-pathogenic; able to accommodate large foreign DNA inse...
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PMID: 17306891
PDF is available here.
Abstract
The multiple inert gas elimination technique (MIGET) represents the gold standard for analysis of ventilation and perfusion distributions in the lung. Modification of this technique allows a much simpler sample processing and hence permits routine clinical application of this technique. MIGET using...
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PMID: 17492417
PDF is available here.
Abstract
We apply global proteome profiling in combination with newly developed quantitative phosphoproteomics approaches for comparative analysis of the cell body (CB) and PD proteome of chemotactic cells. The spatial relationship of 3,509 proteins and 228 distinct sites of phosphorylation were mapped revea...
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PMID: 17494752
PDF is available here.
Abstract
The filtration characteristics of simulated dyeing effluents containing Acid Orange 7, sodium sulfate, and a pH buffer made of acetic acid and sodium acetate is described using a commercially available nanofiltration membrane. The original membrane filtration properties were characterized with deion...
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PMID: 17571839
PDF is available here.
Abstract
Nanofiltration of FVIII at 15 nm is feasible despite the large molecular weight of FVIII and vWF. Nanofiltration has been proven to be highly effective at removing infectious agents while preserving the structural and functional integrity of FVIII....
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PMID: 17456157
PDF is available here.
Abstract
A simple, rapid, and sensitive method for the assay of a sequence-specific DNA-binding protein, nuclear factor-kappaB (NF-kappaB), has been developed by using a DNA-detectable chemiluminogenic reagent and a centrifugal filter that distinguishes different molecular sizes. After the formation of a com...
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PMID: 17374353
PDF is available here.
Abstract
This study extracted the soluble microbial products and loosely bound and tightly bound extracellular polymeric substances (EPS) from suspended sludge from a membrane bioreactor, original and aerobically/anaerobically digested, and compared their fouling potentials on a microfiltration membrane. The...
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PMID: 17115206
PDF is available here.
Abstract
These results suggest that UFDR may be used as a non-destructive tool to monitor biofouling in a wide variety of applications....
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PMID: 17141898
PDF is available here.
Abstract
I European Union (EU) serotype 1 spotted on FTA was shown to be inactivated using specific-pathogen-free (SPF) primary chicken embryo liver cell culture as indicated by absence of cytopathic effect. Sensitivity of the polymerase chain reaction (PCR) test using tenfold dilutions of allantoic fluid fr...
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PMID: 17461276
PDF is available here.