Abstract
We evaluated the BD GeneOhm MRSA achromopeptidase (ACP) assay, which incorporates a new specimen preparation approach. A total of 1,216 leftover nasal samples were tested; using culture as the gold standard, the sensitivity and specificity were 92% and 94.6%, respectively. The new lysis method provid...
|
PMID: 21508148
PDF is available here.
Abstract
To produce anti-19-Nortestosterone (NT) monoclonal antibodies and identify their immunological characteristics.
|
PMID: 21565689
PDF is available here.
Abstract
As a part of a series of yearly meeting, in May 2010 over 40 medical laboratory opinion leaders, pathologists, clinical biochemists and physicians from Europe, Israel and South Africa gathered together in Bardolino, Italy to discuss issues and current challenges for laboratory medicine, including a)...
|
PMID: 21366504
PDF is available here.
Abstract
We estimated the time to clearance of Chlamydia trachomatis (CT) ribosomal RNA (rRNA) after administration of azithromycin for cervical chlamydial infection using APTIMA Combo 2 (Gen-Probe, Inc., San Diego, CA, USA)....
|
PMID: 21371385
PDF is available here.
Abstract
Developments in rapid diagnostic tests (RDTs) have opened new possibilities for improved remote malaria diagnosis that is independent of microscopic diagnosis. Studies in some settings have tried to assess the influence of RDTs on the prescribing behaviour of health workers, but such information is g...
|
PMID: 21297938
PDF is available here.
Abstract
We collected camp clinic records on outpatient malaria diagnoses from 2004 through 2007 and compared the morbidity percentages attributed to malaria during these years, as well as the actual incidence of malaria in 2006 and 2007. Our analyses demonstrate that malaria accounted for 45.8% of all morbi...
|
PMID: 21308578
PDF is available here.
Abstract
To assess the validity of oral mucosal transudate (OMT) specimens for HIV testing in children using enzyme-linked immunosorbent assay (ELISA).
|
PMID: 21626983
PDF is available here.
Abstract
The sensitivity of a rapid test versus microscopy of blood samples was studied while examining 7983 dwellers from the republic's endemic areas. The results of blood testing in the Pyanj and Shurobod districts showed agreement with those of blood microscopy. However, its sensitivity i...
|
PMID: 21932547
PDF is available here.
Abstract
We performed a randomized clinical trial of women using long-acting reversible contraception methods in the Contraceptive CHOICE Project (n=558). Participants were randomly assigned to home-based testing (swabs mailed to the participant's home) or clinic-based testing. Self-collected vaginal swabs w...
|
PMID: 21099596
PDF is available here.
Abstract
We present a fully automated system with pseudo-multiplexing capability for rapid infectious disease diagnosis. The all-in-one system was comprised of a polymer cartridge, a miniaturized thermal cycler, 1-color, 3-chamber fluorescence detectors for real-time reverse transcription polymerase chain re...
|
PMID: 20865195
PDF is available here.
Abstract
We used the direct agglutination test (DAT) and the rK39 ELISA as L. donovani infection markers in 10 VL endemic villages in Nepal. DAT titre distribution showed two subgroups in the population (infected and non-infected individuals), while rK39 did not. The agreement between both tests was moderate...
|
PMID: 21998875
PDF is available here.
Abstract
The authors conducted a study to determine the validity of two commercially available in-office dental unit waterline test kits compared to the gold standard, R2A agar. Samples were collected from the air/water syringes of dental units and cultured on HPC Samplers, Petrifilm AC Plates, and R2A agar...
|
PMID: 21121503
PDF is available here.
Abstract
We have saved valuable time by omitting the pre-isolation step and conclude that DNRA is a rapid, accurate and inexpensive method for direct DST of MTB and may become an appropriate alternative method for the resource limited settings....
|
PMID: 21252446
PDF is available here.
Abstract
The aim of the present prospective study was to assess the diagnostic benefit of UroVysion (Vysis-Abbott Laboratories, Downers Grove, IL) in the follow-up of patients with a history of high-grade non-muscle-invasive urothelial carcinoma of the bladder (NMIBC). An unselected cohort of 25 patients wit...
|
PMID: 20855641
PDF is available here.
Abstract
The sperm-hyaluronan binding assay (HBA) is a diagnostic kit for assessing sperm maturity, function and fertility. The aim of this prospective cohort pilot study was to evaluate the relationship between HBA and WHO sperm parameters (motility, concentration and detailed morphology) and possible influ...
|
PMID: 20860626
PDF is available here.
Abstract
The endemicity status of lymphatic filariasis in Oman is uncertain, with only sporadic cases reported, mostly imported. Immunochromatographic card test surveys were carried out to assess the presence of circulating Wuchereria bancrofti antigenaemia as a marker for active infection in children from s...
|
PMID: 21222423
PDF is available here.
Abstract
We evaluated antigen detection kits for human rotavirus with regard to their usefulness for diagnosing equine rotavirus infection. Limiting dilution analyses showed that of the seven kits investigated the Dipstick `Eiken' Rota (Dipstick) had the highest sensitivity to two serotypes of equine rotavir...
|
PMID: 20453452
PDF is available here.
Abstract
We report on the case of a French citizen who was found dead in his home, 4 days after returning from Cameroon. The patient died of imported malaria, as revealed by the postmortem investigations. Few such cases have been reported throughout the world. This article reviews deaths due to malaria diagn...
|
PMID: 20508488
PDF is available here.
Abstract
We assessed the diagnostic performance of two total PS antigen assays, four free PS assays and three PS activity assays in a group of 28 heterozygous carriers of mutations in PROS1 and 165 control subjects. Several control groups were formed, one of healthy volunteers and - because PS levels are inf...
|
PMID: 20539904
PDF is available here.
Abstract
Point-of-care diagnosis of influenza requires sound recognition of the prevalence of the signs and symptoms of the patients, which would help to define the pattern of clinical presentation of this infection. In severely ill patients, however, clinical features of influenza could be erroneously modif...
|
PMID: 20845739
PDF is available here.
Abstract
A total of 706 urine samples from children in the programme were cultivated. In all 76 children fulfilled the criteria for UTI when culture and symptoms were compared. Dip-slide analysis had a sensitivity of 72% and a negative predictive value of 96%. A total of 329 urine samples with negative dip-s...
|
PMID: 20670589
PDF is available here.
Abstract
These results implied that, to determine whether the patient's sputum contains living AFB, more sensitive techniques should be employed during the follow-up of the patients. These observations suggest that the GTMD method can be useful for early diagnosis of clinically and radiologically suspicious...
|
PMID: 20448064
PDF is available here.
Abstract
We suggest that during the second week of life, reference values should be divided into an early stage and a late stage, at least, for there to be an adequate interpretation of borderline measurements in newborn thyroid screening....
|
PMID: 21073118
PDF is available here.
Abstract
We investigated the adiponectin levels using a number of ELISA assay kits....
|
PMID: 20860176
PDF is available here.
Abstract
Prothrombin time (PT) is widely used as the monitor of oral anticoagulant therapy using ISI/NR system which WHO recommended. However, a main clinical usefulness of an Owren-type combined reagent (TT) also remains in the monitoring of warfarin in Europe and Japan. Recently, a TT reagent utilizing a r...
|
PMID: 20860170
PDF is available here.
Abstract
I (abeta(2)GPI) antibodies are part of the criteria for antiphospholipid syndrome (APS). Therefore they are widely measured and about 30 commercial kits are available.
To investigate the analytical and clinical performances of four fully automated, chemiluminescent assays: HemosIL AcuStar aCL IgG, H...
|
PMID: 20345724
PDF is available here.
Abstract
We estimated the sensitivity and specificity of a commercially available domestic canine rapid diagnostic antigen test for canine parvovirus and a rapid enzyme-linked immunosorbent assay for the detection of antibodies toward Anaplasma phagocytophilum on populations of fishers (Martes pennanti) and...
|
PMID: 20688707
PDF is available here.
Abstract
Study patients were divided into two groups: 61 patients with bacterial infection and 26 patients without it. Semi-quantitative PCT test (cut-off value; > or = 0.5 ng/ml) showed sensitivity of 55.7% and specificity of 84.6% for diagnosis of bacterial infection. The diagnostic value of PCT was higher...
|
PMID: 20715510
PDF is available here.
Abstract
Statistically significant differences were noted between ITB and CD with regard histopathologic criteria: size of granulomas (P = 0.000), giant cells (P = 0.015), caseation necrosis (P = 0.003), confluent granulomas (P = 0.001), discrete granulomas (P = 0.000), and granulomas with lymphoid cuffs (P...
|
PMID: 20503449
PDF is available here.
Abstract
During the 2010 HIV Diagnostics Conference, which took place in Orlando (FL, USA) between 24 and 26 March 2010, salient new data related to three aspects of HIV testing (rapid point-of-care testing, laboratory assays and new technologies) were presented and discussed. A conundrum central to HIV diag...
|
PMID: 20521890
PDF is available here.
Abstract
Various interventions have been implemented to increase the rate of colon cancer screening. The purpose of this study was to determine if persons who are regular patients of a clinic, ages 50-64 years, and not up-to-date with colon cancer screening will complete the at-home fecal-immunochemical test...
|
PMID: 20127156
PDF is available here.
Abstract
We explored the possibility of avoiding this interference by using a different method. We observed that naproxen has no effect on total bilirubin measurement by any method, but O-desmethylnaproxen (the naproxen metabolite) interfered significantly with total bilirubin assays using the Jendrassik and...
|
PMID: 20472845
PDF is available here.
Abstract
A variety of noninvasive molecular approaches to colorectal cancer screening are emerging with potential to improve screening effectiveness and user-friendliness. These approaches are based on the sensitive assay of molecular markers in stool, blood, and urine samples. New methods, especially next g...
|
PMID: 20420950
PDF is available here.
Abstract
The mean bias and limits of agreement for CD4 T-cell measurements between Rea T Count and FACSCount reagents were -16 cells/microl (-4.6%) and -74 to +43, respectively. The correlation obtained was 0.988 with a similarity of 97.9%. Between laboratory variation data was very good with %CV below 10%....
|
PMID: 19847883
PDF is available here.
Abstract
Conventional chromogenic heparin assays are influenced by endogenous plasma AT levels....
|
PMID: 20514621
PDF is available here.
Abstract
We show that the RecTL, N370S allele is a false positive result, demonstrating possible pitfalls of diagnostic kits.
(c) 2010 Elsevier Inc. All rights reserved....
|
PMID: 20096616
PDF is available here.
Abstract
We compared the QuickVue Influenza test with PCR for diagnosing pandemic (H1N1) 2009 in 404 persons with influenza-like illness. Overall sensitivity, specificity, and positive and negative predictive values were 66%, 84%, 84%, and 64%, respectively. Rapid test results should be interpreted cautiousl...
|
PMID: 20409373
PDF is available here.
Abstract
After three weeks of culture, smooth and non-photochromogenic colonies were formed. The niacin accumulation test was negative. The homologies of DNA sequence between the new strains and M. tuberculosis for 16S rRNA and rpoB genes were 97.8% and 90.2%, respectively. The tests with TRC and MTD kits we...
|
PMID: 20560401
PDF is available here.
Abstract
The aim of this retrospective study was to evaluate the Immunoquick+4 (BioSynex, Strasbourg, France), a three-band malaria rapid diagnostic test (MRDT) targeting histidine-rich protein-2 (HRP-2) and pan Plasmodium-specific parasite lactate dehydrogenase, in a non-endemic reference setting. Stored wh...
|
PMID: 20232100
PDF is available here.
Abstract
DNA can be extracted in sufficient amounts from Salivette with a fully automated system with a short turnaround time. Real-time PCR can be performed with these samples....
|
PMID: 20218901
PDF is available here.
Abstract
DBS EasyQ VL assays provide an accurate option for early infant diagnosis in low resource settings.
Copyright 2010 Elsevier B.V. All rights reserved....
|
PMID: 20211580
PDF is available here.
Abstract
The OraQuick HCV Rapid Antibody Test appears suitable as an aid in the diagnosis of HCV infection.
Copyright 2010 Elsevier B.V. All rights reserved....
|
PMID: 20362493
PDF is available here.
Abstract
The AMPLICOR HPV Test can be successfully and reproducibly performed from DNA isolated from residual SurePath cervical cytology specimens stored at ambient temperature for at least 21 days. This provides clinical laboratories flexible storage conditions for residual SurePath cytology specimens.
Copy...
|
PMID: 20233675
PDF is available here.
Abstract
The recent emergence of a novel H1N1 influenza A virus in humans caused the first influenza pandemic of this century. Many clinical diagnostic laboratories are overwhelmed by the testing demands related to the infection. Three novel H1N1-specific primer-probe sets reported during the early phase of...
|
PMID: 20138917
PDF is available here.
Abstract
Until recently the NucliSens EasyQ HIV-1 V1.2 system has been used throughout South Africa as part of the national antiretroviral roll-out programme for the monitoring of HIV-1 RNA load in patients on antiretroviral treatment. Shortly after changing to a new assay lot number an increased proportion...
|
PMID: 20219541
PDF is available here.
Abstract
Clinical specificity, analytical and clinical sensitivities, reproducibility, and subtype/genotype coverage of the cobas TaqScreen MPX Test, a multiplex nucleic acid test with expanded coverage of HIV variants, were determined. A total of 72,281 blood donations were evaluated. The 95% limit of detec...
|
PMID: 20152864
PDF is available here.
Abstract
Anticoagulant control facilities are being overwhelmed by requests for monitoring and large numbers of patients are not therefore receiving treatment. Procedures designed for point-of-care testing have therefore been developed, the most popular being the CoaguChek. The need for external quality asse...
|
PMID: 20216990
PDF is available here.