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Amniotic Fluid (0):
Recent article
Amniotic Fluid
Abstract
To evaluate the effect of human amniotic fluid (HAF) on retinal pigmented epithelial cells growth and trans-differentiation into retinal neurons, retinal pigmented epithelium (RPE) cells were isolated from neonatal human cadaver eye globes and cultured in Dulbecco's modified Eagle's...
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PMID: 21142973
PDF is available here.
PDF is available here.
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Stem Cell Research (7)1 2011
Abstract
Cell based therapies for bone regeneration are an exciting emerging technology, but the availability of osteogenic cells is limited and an ideal cell source has not been identified. Amniotic fluid-derived stem cells (AFS) and bone-marrow derived mesenchymal stem cells (MSCs) were com...
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PMID: 21531647
PDF is available here.
PDF is available here.
Abstract
Cell therapy has developed as a complementary treatment for myocardial regeneration. While both autologous and allogeneic uses have been advocated, the ideal candidate has not been identified yet. Amniotic fluid-derived stem (AFS) cells are potentially a promising resource for cell t...
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PMID: 21120638
PDF is available here.
PDF is available here.
Abstract
We show that AFCs express pluripotency markers (Nanog, Sox2, and Oct3/4) as well as retinal transcription factor genes (Et, Lhx2, Tll1, Six6, Otx2, Pax6, and Fgf15). AFCs from amniotic fluid of Fgf15.gfp, Nrl.gfp, and Crx.gfp embryos cultured in retinal proliferation and differentiation conditions f...
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PMID: 20939691
PDF is available here.
PDF is available here.
Irish medical journal (104)7 2011
Abstract
Fetal pleural effusion is a rare occurrence, with an incidence of 1 per 10-15,000 pregnancies. The prognosis is related to the underlying cause and is often poor. There is increasing evidence that in utero therapy with thoraco-amniotic shunting improves prognosis by allowing lung expansion thereby pr...
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PMID: 21957687
PDF is available here.
PDF is available here.
Abstract
We demonstrate that nanofiber meshes are able to support these cell functions robustly, with both cell types demonstrating strong osteogenic potential. Differences in the kinetics of osteogenic differentiation were observed between hMSCs and hAFS cells, with the hAFS cells displaying a delayed alkal...
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PMID: 20504075
PDF is available here.
PDF is available here.
Biology of Reproduction (83)4 2010
Abstract
We examined whether erythromycin would eradicate intra-amniotic ureaplasma infections in pregnant sheep. At Gestational Day (GD) 50 (term, GD 150), pregnant ewes received intra-amniotic injections of erythromycin-sensitive Ureaplasma parvum serovar 3 (n = 16) or 10B medium (n = 16). At GD 100, amnio...
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PMID: 20610808
PDF is available here.
PDF is available here.
Abstract
Our potential for further testing is limited; thus far, no other congenital disorders of glycosylation-positive samples have been available. Verification of our results in another laboratory with the exclusion of several potentially pertinent variables is advisable....
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PMID: 20528096
PDF is available here.
PDF is available here.
Abstract
Our results showed the presence of multiple neuronal markers after growth in serum-containing medium [TUJ1, MAP2, NF-M, TH (tyrosine hydroxylase)], which was significantly up-regulated after serum withdrawal in NB medium alone with induction of NeuN (neuronal nuclei) and NSE (neuron-specific enolase...
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PMID: 20388119
PDF is available here.
PDF is available here.
Abstract
OBJECTIVE: Angiogenesis is critical for successful pregnancy. An anti-angiogenic state has been implicated in preeclampsia, fetal growth restriction and fetal death. Increased maternal plasma concentrations of the anti-angiogenic factor, soluble vascular endothelial growth factor receptor (sVEGFR)-1...
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PMID: 20199197
PDF is available here.
PDF is available here.
Abstract
A quantitative fluorescence polymerase chain reaction (QF-PCR) technique based on the determination of triple-dose chromosome-specific short tandem repeats (STR) has been recently developed for the prenatal diagnosis of numeral abnormalities of chromosomes 21, 18, 13 and X and Y. This investigation...
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PMID: 20891040
PDF is available here.
PDF is available here.
Abstract
Caesarean birth, without prior labour, is associated with an increased risk of neonatal respiratory morbidity among term infants. The concentration of lamellar bodies in amniotic fluid reflects pulmonary surfactant production and release, and is thus used in preterm populations as a marker of fetal...
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PMID: 20716264
PDF is available here.
PDF is available here.
Abstract
Obstetrics & Gynecology (116)2 Pt 1 2010
Abstract
We searched electronic databases of the human literature in PubMed, EMBASE, and the Cochrane Library up to February 2010 using the following key words: "preterm/pre-term + (birth/delivery)" and "cytokine" or "inflammation/inflammatory + marker/biomarker."
We included observational studies that repor...
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PMID: 20664401
PDF is available here.
PDF is available here.
Abstract
To evaluate oxidative stress markers and prolidase activity in the amniotic fluid of fetuses with a neural tube defect (NTD) compared to the amniotic fluid of normal fetuses.
Amniotic fluid samples from 36 pregnancies of fetuses with NTD were compared with the amniotic fluid from 36 pregnancies of g...
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PMID: 20516661
PDF is available here.
PDF is available here.
Abstract
Ang-1, Ang-2, and sTie2 are physiological constituents of AF that are GA and IAI regulated. Ang-2/Ang-1 ratio may play a role in modulating the fetal inflammatory response to IAI. Placental sTie2 shedding likely involves a Golgi-mediated mechanism....
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PMID: 20410222
PDF is available here.
PDF is available here.
Abstract
The incidences of the abnormal tests were 1.5% and 6.0% in FBP and rBPP, respectively. The data showed a positive correlation between the two tests (r(s) = 0.67; p < 0.01). Regarding the operative time, FBP assessment was 25.56 +/- 8.75 times longer than rBPP. The number of abnormal NST was remarked...
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PMID: 20649052
PDF is available here.
PDF is available here.
Secretory profiles and wound healing effects of human amniotic fluid-derived mesenchymal stem cells.
Abstract
We examined the paracrine factors released by human amniotic fluid-derived mesenchymal stem cells (AF-MSCs) and their ability to accelerate the wound-healing process by stimulating proliferation and migration of dermal fibroblasts. AF-MSCs expressed the typical MSC marker proteins CD13, CD29, and CD...
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PMID: 19686050
PDF is available here.
PDF is available here.
Abstract
Down syndrome (DS) is an abnormality of the 21st chromosome that commonly occurs in children born to advanced age women. Amniotic fluid (AF) is usually collected from such women for prenatal diagnosis. This study analyzed human AF supernatants (AFS) by mass spectrometric (MS) approach to search for...
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PMID: 20622492
PDF is available here.
PDF is available here.
Abstract
The aim of this study was to verify the existence of a side population (SP) of cells in second-trimester amniotic fluid.
Amniotic fluid samples (n = 35) were obtained, and the number and size of viable amniotic fluid cells (AFCs) were analyzed. Small AFCs (SAFCs) and large AFCs (LAFCs) were isolated...
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PMID: 20708517
PDF is available here.
PDF is available here.
Abstract
To present a prenatal diagnosis of congenital cytomegalovirus (CMV) infection in a pregnancy with fetal ascites.
A 33-year-old, gravida 6, para 2, woman was referred to a hospital at 20 weeks of gestation for management of fetal ascites. The woman had not experienced recent rubella or herpes simplex...
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PMID: 20708531
PDF is available here.
PDF is available here.
Reproductive Toxicology (29)3 2010
Abstract
We report a case report regarding a woman, who accidentally was treated with Candesartan during the first 22 weeks of pregnancy. At 22 weeks of gestation the fetal kidneys were oedematous with very little amniotic fluid. The prognosis for the fetus was considered poor. Five weeks after discontinuati...
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PMID: 20132879
PDF is available here.
PDF is available here.
Biological Psychiatry (67)11 2010
Maternal prenatal cortisol and infant cognitive development: moderation by infant-mother attachment.
Abstract
These findings mimic experimental animal findings and provide the first direct human evidence that increased cortisol in utero is associated with impaired cognitive development, and that its impact is dependent on the quality of the mother-infant relationship.
Copyright 2010 Society of Biological Ps...
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PMID: 20188350
PDF is available here.
PDF is available here.
Minerva Pediatrica (62)3 Suppl 1 2010
Abstract
Chorioamnionitis (CA) is defined as an infection that can affect amniotic fluid, placenta and uterus. The chorioamnionitis is present in 10-40% of cases of maternal peripartum fever and in 50% of preterm labor. Diagnosis is based on the presence of maternal fever (>38 degrees C) at least 2 of these...
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PMID: 21090085
PDF is available here.
PDF is available here.
Minerva Pediatrica (62)3 Suppl 1 2010
Abstract
We present our experience on the relationship between histological chorioamnionitis, preterm birth and inflammatory diseases of VLBW infants....
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PMID: 21090086
PDF is available here.
PDF is available here.
Minerva Pediatrica (62)3 Suppl 1 2010
Abstract
Our data show that preterm newborns, also at the lower GA, are able to produce defenses, underlining that their innate defence system is already active before the at-term delivery date....
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PMID: 21089718
PDF is available here.
PDF is available here.
Abstract
Amniotic fluid was collected from 78 pregnant women at birth additionally with their urine prior to delivery as well as neonatal urine and meconium. The smoking markers, nicotine and its metabolites cotinine and trans-3'-hydroxycotinine (OH-cotinine), were determined using high-performance liquid ch...
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PMID: 20164157
PDF is available here.
PDF is available here.
Arerugi = [Allergy] (59)5 2010
Abstract
Ginekologia polska (81)5 2010
Abstract
Analysis of PCT and IL-6 concentrations in serum allows to identify women that are more likely to deliver newborns with symptoms of severe infection. In cases of severe infection, the usefulness of PCT measurement surpasses any currently used methods, such as CRP concentration measurement, for examp...
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PMID: 20568512
PDF is available here.
PDF is available here.
Abstract
1) Cultivation techniques for ureaplasmas did not detect most cases of intra-amniotic infection caused by these microorganisms (91% of cases with cervical insufficiency and microbial footprints for ureaplasmas in the amniotic cavity had a negative AF culture); 2) Patients with a negative AF culture...
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PMID: 20192887
PDF is available here.
PDF is available here.
Abstract
Patients with placenta previa presenting with vaginal bleeding have intra-amniotic infection in 5.7% of the cases, and IAI in 17.9%. IAI in patients with placenta previa and vaginal bleeding is a risk factor for preterm delivery within 48 h....
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PMID: 20146660
PDF is available here.
PDF is available here.
Pediatric Research (67)5 2010
Abstract
We 1) described normal levels of 2ME2 in maternal blood, cord blood, breast milk, and amniotic fluid, and 2) compared a composite measure of perinatal outcome between infants born with high and low levels of 2ME2. We found that 2ME2 was significantly decreased in all fluids compared with prepartum m...
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PMID: 20098341
PDF is available here.
PDF is available here.
Abstract
Total deliveries during the study period were 960. One hundred and twenty two (12.7%) cord results were studied. Umbilical cord arterial base excess (> or = 12 mmol/L) at birth was used as gold standard to determine the validity of cardiotocography and meconium staining of amniotic fluid. Validity t...
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PMID: 20527603
PDF is available here.
PDF is available here.
Abstract
We generated human skin equivalents (HSEs) under submerged conditions mimicking the aqueous in utero environment and investigated the morphology and differentiation process of the formed epidermis. Further, the skin barrier, which resides in the stratum corneum (SC), was characterized by its lipid c...
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PMID: 19929321
PDF is available here.
PDF is available here.
Abstract
A residual AFI < 50 mm after preterm PROM between 24 and 34 weeks of gestation, which is mostly seen in grand multiparous women in Eastern Turkey, may be a valuable prognostic variable for anticipating adverse maternal and neonatal outcomes....
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PMID: 19521709
PDF is available here.
PDF is available here.
Abstract
Biomarkers of intrauterine inflammation such as interleukin-6, although sensitive, are not specific. Thus, decision to deliver remote from term because of intrauterine infection and/or inflammation should be based on clinical signs and/or bacterial culture or Gram stain of amniotic fluid. In patient...
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PMID: 20139764
PDF is available here.
PDF is available here.
Abstract
During pregnancy, the maternal, placental and fetal physiological characteristics constantly evolve and thereby constantly alter drug bioavailability in the mother and feto-placental unit. Gastric emptying time is increased and bowel movements are reduced. Distribution in the maternal body is mainly...
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PMID: 20347563
PDF is available here.
PDF is available here.
Journal of Neurosurgery (112)4 2010
Abstract
The GDNF-modified human AFMSCs appeared to promote nerve regeneration. The consecutive expression of GDNF was demonstrated in GDNF-modified human AFMSCs up to 4 weeks. These findings support a nerve regeneration scenario involving cell transplantation with additional neurotrophic factor secretion....
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PMID: 19817545
PDF is available here.
PDF is available here.
Abstract
We investigated whether these human SC could efficiently differentiate into myogenic lineage in vitro and integrate in vivo skeletal muscle in severe combined immunodeficiency (SCID) mice. C/kit immunomagnetic-sorted AF (AF c/kit+) SC were characterized by immunocytochemistry and Southern blotting f...
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PMID: 19730985
PDF is available here.
PDF is available here.
Abstract
PTX3 is a physiologic constituent of the AF, and its median concentration is elevated in the presence of IAI, suggesting that PTX3 may play a role in the innate immune response against IAI....
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PMID: 19792835
PDF is available here.
PDF is available here.
Abstract
Abstract
We extended previous studies dealing with the androgen theory of autism and examined sex ratios in the siblings of 326 individuals with ASD (245 males, 81 females) who had been consecutively assessed at two Danish university clinics of child psychiatry during the 25-year period from 1960 to 1985....
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PMID: 19549197
PDF is available here.
PDF is available here.
Abstract
Elevated mid-trimester concentrations of AF IL-6, or/and of TNF-alpha can identify women at risk for intra-amniotic infection and for spontaneous PTD.
2009 Elsevier Ireland Ltd. All rights reserved....
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PMID: 19945777
PDF is available here.
PDF is available here.
Abstract
To avoid pitfalls in assessing amniotic fluid for twins, the intertwin membrane should be shown in every image that is used to document fluid ascribed to each twin, and images at right angles to the initial image can help identify an amniotic wrinkle....
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PMID: 20103796
PDF is available here.
PDF is available here.
[Delivery room practices in infants born through meconium stained amniotic fluid: a national survey]
Abstract
In spite of not being currently recommended, intrapartum suction and postnatal intubation and suction of vigorous infants with MSAF are commonly performed in Argentina while in depressed newborns the latter procedure is not always carried out. Our study confirms the frequent gaps observed between sc...
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PMID: 20204237
PDF is available here.
PDF is available here.
Abstract
sTREM-1 is a physiologic constituent of the AF, and its concentration: (1) is significantly elevated in the presence of IAI; (2) increases with advancing gestation; and (3) does not change in the presence of spontaneous labor at term. We propose that sTREM-1 play a role in the innate immune response...
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PMID: 19591072
PDF is available here.
PDF is available here.
Analytical Sciences (26)10 2010
Abstract
These results can improve our understanding of the mechanical properties of hAFSCs during osteogenic differentiation. AFM can be used as a powerful tool for detecting ultrastructures and mechanical properties....
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PMID: 20953044
PDF is available here.
PDF is available here.
Abstract
We have shown that epithelial markers are observed in AF stem cells at the same time with mesenchymal (Keratin 19, Keratin 18, and p63). During cloning, colonies of cells with fibroblastlike and epithelial-like morphologies are formed. The status and differentiation potential of stem cells from AF h...
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PMID: 21077360
PDF is available here.
PDF is available here.
Abstract
About 30% of monochorionic twin pregnancies are complicated by twin-to-twin transfusion syndrome (TTTS), isolated discordant growth, twin anemia-polycythemia sequence, congenital defects or intrauterine demise. About 15% will be eligible for invasive fetal therapy, either fetoscopic laser treatment...
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PMID: 20413975
PDF is available here.
PDF is available here.
Abstract
We hypothesized that fetal lipopolysaccharide exposures to the chorioamnion, lung, or gut would induce distinct systemic inflammatory responses.
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PMID: 19801145
PDF is available here.
PDF is available here.
Abstract
The prenatal diagnosis of Bartter syndrome can be based on the high chloride level in the amniotic fluid. Microscopic examination of the placenta in untreated cases showed extensive mineralization in the chorionic villi in previous studies. Two cases were presented at 26-29 weeks of gestation with s...
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PMID: 20450264
PDF is available here.
PDF is available here.
Abstract
Light transmission through amniotic fluid is optimal in the near-infrared spectrum and at the highest visible wavelengths, regardless of gestational age. Overall light transmission through amniotic fluid decreases throughout gestation. The light source and camera of videofetoscopy systems should be...
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PMID: 19940448
PDF is available here.
PDF is available here.
Abstract
We report a case of fetal ascites and umbilical arterial necrotic vasculopathy, an association not previously described.
2010 S. Karger AG, Basel....
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PMID: 20130389
PDF is available here.
PDF is available here.
Abstract
1) Pregnancies with an IUD were associated with a higher rate of late miscarriage, preterm delivery, vaginal bleeding, clinical chorioamnionitis, and placental abruption than those without an IUD; 2) among patients with available histologic examination of the placenta, the rate of histologic chorioa...
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PMID: 19650756
PDF is available here.
PDF is available here.
Abstract
Patients with a positive culture had a higher frequency of oligohydramnios and a lower median AFI than those with a negative culture but with intra-amniotic inflammation (P<0.01). However, there was no significant difference in the median AFI or in the frequency of oligo-hydramnios according to the...
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PMID: 19708825
PDF is available here.
PDF is available here.
Abstract
We propose that sHLA-G may participate in the regulation of the host immune response against intra-amniotic infection....
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PMID: 19916713
PDF is available here.
PDF is available here.
Abstract
Sixteen of 21 metabolite concentrations differed significantly between 2nd and 3rd trimester groups. Betaine (0.00846+/-0.00206 mmol/kg vs. 0.0133+/-0.0058 mmol/kg, P < 0.002) and creatinine (0.0124+/-0.0058 mmol/kg vs. 0.247+/-0.011 mmol/kg, P < 0.001) concentrations increased significantly, while...
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PMID: 19779747
PDF is available here.
PDF is available here.
Abstract
Abstract
A total of 210 samples were collected. According to the SDF-1alpha cutoff value established by the receiver operating characteristic curve analysis (< 6.42 vs. > or = 6.42 pg/mL), there was a trend toward higher preterm birth rate, lower birth weight and lower 1-minute and 5-minute Apgar scores when...
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PMID: 20028644
PDF is available here.
PDF is available here.
Biomedical Research (30)6 2009
Abstract
We demonstrate that a cyanine dye, lumin, significantly suppressed cytopathic effect by herpes simplex virus (HSV)-1 toward human amnionic FL cell and also it reduced replication of HSV-1 in a dose-dependent manner. In addition, lumin additively augmented the antiviral effect of interferon (IFN)-alp...
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PMID: 20051646
PDF is available here.
PDF is available here.