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Yeasts (23):
Recent article
Yeasts
Abstract
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Fungal Biology (115)10 2011
Abstract
Our aim was to investigate the response of selected yeasts and yeast-like fungi from extreme environments to various temperatures at the level of their plasma membranes, in order to elucidate the connections between their plasma-membrane fluidity (measured by electron paramagnetic resonance spectro...
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PMID: 21944207
PDF is available here.
PDF is available here.
Biomacromolecules (12)6 2011
Abstract
We report an approach for the fabrication of fully biodegradable self-rolled tubes based on patterned polysuccinimide/polycaprolactone bilayers. These polymers are biocompatible, biodegradable, produced industrially, and are already approved for biomedical purposes. Both polycaprolactone and polysuc...
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PMID: 21524116
PDF is available here.
PDF is available here.
PLoS Biology (9)6 2011
Abstract
We show here that mammalian cells lacking High Mobility Group Box 1 protein (HMGB1) contain a reduced amount of core, linker, and variant histones, and a correspondingly reduced number of nucleosomes, possibly because HMGB1 facilitates nucleosome assembly. Yeast nhp6 mutants lacking Nhp6a and -b pro...
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PMID: 21738444
PDF is available here.
PDF is available here.
DNA Repair (10)5 2011
Abstract
We use two protein footprinting techniques, limited proteolysis and oxidative surface mapping, coupled with mass spectrometry to identify amino acids involved along the DNA-binding surface of the Pms1-NTD. Limited proteolysis experiments elucidated several basic residues that were protected in the p...
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PMID: 21354867
PDF is available here.
PDF is available here.
FEMS Yeast Research (11)3 2011
Abstract
We investigate whether this gradual transition also occurs at the intracellular level by quantifying the intracellular metabolism of Saccharomyces cerevisiae, Saccharomyces bayanus, Saccharomyces exiguus, Kluyveromyces thermotolerans, Yarrowia lipolytica, Pichia angusta and Candida rugosa by (13)C-f...
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PMID: 21205161
PDF is available here.
PDF is available here.
Abstract
Abstract
We take advantage of yeast cells wherein the neuronal CPEB of Aplysia is expressed in the absence of any neuronal factors and can stably adopt either an active or an inactive state. Reminiscent of well-characterized yeast prions, we find that CPEB can adopt several distinct activity states or "strai...
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PMID: 21270333
PDF is available here.
PDF is available here.
Comptes Rendus Biologies (334)8-9 2011
Abstract
Over the decades, basic research in life sciences has profited greatly from the study of the small unicellular fungal species Saccharomyces cerevisiae. This yeast turned out to be key for the identification and understanding of molecular mechanisms that underlay the basic functions o...
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PMID: 21819940
PDF is available here.
PDF is available here.
Abstract
We systematically queried the Saccharomyces cerevisiae proteome for Prp19 WD40 domain interaction partners by two-hybrid analysis. We report that in addition to S. cerevisiae Cwc2, the splicing factor Prp17 binds directly to the Prp19 WD40 domain in a 1:1 ratio. Prp17 binds simultaneously with Cwc2...
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PMID: 21386897
PDF is available here.
PDF is available here.
Abstract
We have investigated the use of appropriate evolutionary models to address inter-gene heterogeneities and the scalability and validity of supermatrix analysis as the phylogenetic problem becomes more difficult and the number of genes analysed approaches truly phylogenomic dimensions. We have extende...
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PMID: 21850235
PDF is available here.
PDF is available here.
Comptes Rendus Biologies (334)8-9 2011
Abstract
This short article presents an overview of tandem gene arrays (TGAs) in hemiascomycete yeasts. In silico and in vivo analyses are combined to address structural, functional and evolutionary aspects of these particular chromosomal structures. Genomic instability of TGAs is discussed....
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PMID: 21819945
PDF is available here.
PDF is available here.
Abstract
We propose an imputation scheme based on nonlinear dependencies between genes. By simulations based on real microarray data, we show that incorporating nonlinear relationships could improve the accuracy of missing value imputation, both in terms of normalized root-mean-squared error and in terms of...
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PMID: 20733236
PDF is available here.
PDF is available here.
Comptes Rendus Biologies (334)8-9 2011
Abstract
DNA sequencing has revolutionized yeast taxonomy. Although initially rDNA sequences proved to be universal and convenient for assigning phylogenetic relationships, it was eventually supplanted by multigene analysis, which provided more discriminating and robust results. This led to a...
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PMID: 21819939
PDF is available here.
PDF is available here.
Comptes Rendus Biologies (334)8-9 2011
Abstract
We review recent progress in the exploration of the intraspecific diversity using large collections of yeast isolates. These recent large-scale polymorphism surveys have increased our understanding of the population structures as well as the evolutionary history of the species. In addition, these re...
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PMID: 21819942
PDF is available here.
PDF is available here.
Comptes Rendus Biologies (334)8-9 2011
Abstract
We use the experimentally determined mutational rate of Saccharomyces cerevisiae to calculate the numbers of successive generations corresponding to observed sequence polymorphism between strains or species of other yeasts. We then examine synteny conservation across the entire subphylum of Saccharo...
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PMID: 21819943
PDF is available here.
PDF is available here.
Comptes Rendus Biologies (334)8-9 2011
Abstract
We studied synteny conservation between 18 yeast species and 13 vertebrate species in order to provide a comparative analysis of the chromosomal plasticity in these 2 phyla. By computing the regions of conserved synteny between all pairwise combinations of species within each group, we show that in...
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PMID: 21819944
PDF is available here.
PDF is available here.
Comptes Rendus Biologies (334)8-9 2011
Abstract
The steady-state mRNA level is the result of two opposing processes: transcription and degradation; both of which can provide important points to regulate gene expression. In the model organism yeast Saccharomyces cerevisiae, it is now possible to determine, at the genomic level, the...
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PMID: 21819946
PDF is available here.
PDF is available here.
Comptes Rendus Biologies (334)8-9 2011
Abstract
Gene regulation is a major source of phenotypic diversity between and within species. This aspect of evolution has long been addressed from the sole point of view of the genome sequence. The incredible development of transcriptomics approaches now allows one to actually study the top...
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PMID: 21819947
PDF is available here.
PDF is available here.
Comptes Rendus Biologies (334)8-9 2011
Abstract
With the development of new sequencing technologies in the past decade, yeast genomes have been extensively sequenced and their structures investigated. Transposable elements (TEs) are ubiquitous in eukaryotes and constitute a limited part of yeast genomes. However, due to their abil...
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PMID: 21819950
PDF is available here.
PDF is available here.
Abstract
We report the development of a yeast expression system for PfHsp70-1, a P. falciparum cytoplasmic chaperone. We found that PfHsp70-1 repairs mutant growth phenotypes in yeast strains lacking the two primary cytosolic Hsp70s, SSA1 and SSA2, and in strains harboring a temperature sensitive SSA1 allele...
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PMID: 21625512
PDF is available here.
PDF is available here.
Abstract
Yeast ribosomal proteins L11 and S18 form a dynamic intersubunit interaction called the B1b/c bridge. Recent high resolution images of the ribosome have enabled targeting of specific residues in this bridge to address how distantly separated regions within the large and small subunit...
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PMID: 21625514
PDF is available here.
PDF is available here.
Abstract
Our method performs as well as GRIMM-Synteny on mammalian genomes, and outperforms it for clades with much greater evolutionary distances such as the Hemiascomycetous yeasts....
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PMID: 21441096
PDF is available here.
PDF is available here.
Abstract
We describe a novel approach to predict the structure of key TR features and to aid the identification of TRs in genomes, using a program we developed, TRFolder. We applied our method to confirm and improve previously studied core structures from Saccharomyces and Kluyveromyces TRs. We made novel st...
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PMID: 21441097
PDF is available here.
PDF is available here.
Abstract
We have recently shown that, in vivo, glucose regulates V-ATPase through cytosolic pH, suggesting that V-ATPase contains a pH sensitive subunit, which regulates assembly of the holo-complex.(2) Here, we present the purification and biochemical characterization of the N-terminal domain of subunit 'a'...
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PMID: 21057203
PDF is available here.
PDF is available here.
Voprosy pitaniya (80)1 2011
Abstract
The principle of action, advantages and different types of Petrifilm as alternative test to the classical microbiological analysis are considered. Petrifilm is up-to-date high technology test systems for the rapid quantitative microbiological control in foodstuff of sanitary-significant and pathogeni...
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PMID: 21574466
PDF is available here.
PDF is available here.
Waste Management (31)1 2011
Abstract
Our results showed a potential viable and economic use of vinasse.
Copyright © 2010 Elsevier Ltd. All rights reserved....
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PMID: 20864326
PDF is available here.
PDF is available here.
Biocontrol Science (15)4 2010
Abstract
Our data reveal that the new method considerably shortens the time required for detection of yeasts and molds in food....
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PMID: 21212506
PDF is available here.
PDF is available here.
FEMS Yeast Research (10)7 2010
Abstract
We identified 177 ORFs and 56 proteins among those most expressed during the process, thus highlighting cell stress response, mitochondrial and carbohydrate metabolism as the most represented functional categories. A direct correlation between mRNA changes and protein abundance was observed for seve...
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PMID: 20738407
PDF is available here.
PDF is available here.
Abstract
We focus particularly on the various signaling pathways that control the activity of the enzymes of glycogen storage. We also discuss our recent understanding of the important role played by the vacuole in glycogen metabolism. In the case of bacterial glycogen, special emphasis is placed on aspects...
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PMID: 20412306
PDF is available here.
PDF is available here.
Abstract
We describe a rapid yeast bioassay for androgenic and anti-androgenic compounds based on androgen-dependent β-catenin-enhanced N-C interaction. The bioassay was also effective at detecting compounds that inhibit the N-C interaction in ways that do not involve binding to the ligand-binding domain....
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PMID: 20834141
PDF is available here.
PDF is available here.
Cell Host & Microbe (8)3 2010
Abstract
We demonstrate that oral epithelial cells orchestrate an innate response to C. albicans via NF-κB and a biphasic MAPK response. Activation of NF-κB and the first MAPK phase, constituting c-Jun activation, is independent of morphology and due to fungal cell wall recognition. Activation of the sec...
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PMID: 20833374
PDF is available here.
PDF is available here.
Kidney International (67) 2010
Abstract
Kidney International (78)10 2010
Abstract
Kidney International (78)10 2010
Abstract
Kidney International (78)10 2010
Abstract
Kidney International (78)10 2010
Abstract
Abstract
To analyze the biodiversity and distribution of the yeast species of Qula in Tibet and milk cake in Yunnan, and to provide essential data for the utilization of yeasts in the traditional dairy products of China.
Forty-one yeast strains were isolated from 5 samples of Qula in Tibet and 8 samples of m...
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PMID: 21090253
PDF is available here.
PDF is available here.
Abstract
To determine which yeasts are present in the naturally fermented milks of China, 69 samples made by the nomads of Tibet were collected from the Tibetan Plateau in China. From these samples, 225 strains of yeast were isolated and identified using conventional microbiological analysis and gene sequenc...
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PMID: 20921981
PDF is available here.
PDF is available here.
Abstract
We propose a model to be used for statistical unsupervised CLSM image segmentation. The model is derived from the CLSM image formation mechanics and its performance is compared to the existing alternatives. Results show that it provides a much better description of the data on classes characterized...
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PMID: 20363677
PDF is available here.
PDF is available here.
Abstract
Three new aromatic epsilon-lactones, aggregatins A (1), B (2), and C (3), a new naphthoquinone derivative, aggregatin D (4), and three known anthraquinones, 2-methylanthraquinone, 7-methoxy-2-methylanthraquinone, and 7-hydroxy-2-methylanthraquinone, were isolated from the tubers of Sinningia aggrega...
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PMID: 20684540
PDF is available here.
PDF is available here.
Abstract
We present RNABuilder, a novel code that uses internal coordinate mechanics to satisfy user-specified base pairing and steric forces under chemical constraints. The code recapitulates the topology and characteristic L-shape of tRNA and obtains an accurate noncrystallographic structure of the Tetrahy...
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PMID: 20651028
PDF is available here.
PDF is available here.
Journal of Biological Chemistry (285)33 2010
Abstract
The nucleotide state of actin (ATP, ADP-P(i), or ADP) is known to impact its interactions with other actin molecules upon polymerization as well as with multiple actin binding proteins both in the monomeric and filamentous states of actin. Recently, molecular dynamics simulations predicted that a se...
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PMID: 20530485
PDF is available here.
PDF is available here.
Abstract
We decided to evaluate the immunological effects of two different types of humic acids, differing in source and biochemical characteristics. Using both components either alone or in combination with the well-established yeast-derived immunomodulator glucan, we measured their effects on both the cell...
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PMID: 20553181
PDF is available here.
PDF is available here.
Abstract
Abstract
I (Fe(+4)=O, porph+(.)) or Compound ES (Fe(+4)=O, Trp+(.)). R48A/W191F formed a Fe(+2)O2 complex upon photolysis of Fe(+2)CO in the presence of O2, and N-hydroxyguanidine was observed to react with this species to produce products, distinct from N-nitrosoguanidine, that gave a positive Griess reacti...
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PMID: 20346907
PDF is available here.
PDF is available here.
Abstract
Single-celled organisms monitor cell geometry and use this information to control cell division. Such geometry-sensing mechanisms control both the decision to enter into cell division and the physical orientation of the chromosome segregation machinery, suggesting that signals controlling cell divis...
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PMID: 20655459
PDF is available here.
PDF is available here.
Plant Physiology (153)3 2010
Abstract
We found that a member of the DOF (for DNA binding with one finger) transcription factor family, Arabidopsis (Arabidopsis thaliana) DOF4.7, was expressed robustly in the abscission zone. The Arabidopsis 35S::AtDOF4.7 lines with constitutive expression of AtDOF4.7 exhibited an ethylene-independent fl...
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PMID: 20466844
PDF is available here.
PDF is available here.
Molecular Plant (3)4 2010
Abstract
We will focus on higher-order chromatin organization in yeast with respect to the nuclear envelope and nucleolus. In Arabidopsis thaliana, a model plant for which we have a complete genome sequence, chromosome territory (CT) arrangement and somatic homologous pairing in interphase nuclei seem to occ...
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PMID: 20601371
PDF is available here.
PDF is available here.
Pharmaceutical Biology (48)7 2010
Abstract
We used a modified recent technique to evaluate the antifungal activity only of the volatile parts at doses from 5 to 20 microL of pure essential oil. Antifungal testing showed that Cuminum cyminum is active in general on all fungi but in particular on the dermatophytes, where Trichophyton rubrum wa...
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PMID: 20645785
PDF is available here.
PDF is available here.
Abstract
We focused on the 79 residue Sna2p protein. We found that Sna2p is localized in the vacuolar membrane. Directed mutagenesis showed that two functional tyrosine motifs YXXØ are present in the C-terminal region. Each of these is involved in a different Golgi-to-vacuole targeting pathway: the tyrosine...
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PMID: 20406419
PDF is available here.
PDF is available here.
Abstract
We focused on the 79 residue Sna2p protein. We found that Sna2p is localized in the vacuolar membrane. Directed mutagenesis showed that two functional tyrosine motifs YXXØ are present in the C-terminal region. Each of these is involved in a different Golgi-to-vacuole targeting pathway: the tyrosine...
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PMID: 20406419
PDF is available here.
PDF is available here.
Abstract
We focused on the 79 residue Sna2p protein. We found that Sna2p is localized in the vacuolar membrane. Directed mutagenesis showed that two functional tyrosine motifs YXXØ are present in the C-terminal region. Each of these is involved in a different Golgi-to-vacuole targeting pathway: the tyrosine...
|
PMID: 20406419
PDF is available here.
PDF is available here.
Abstract
We focused on the 79 residue Sna2p protein. We found that Sna2p is localized in the vacuolar membrane. Directed mutagenesis showed that two functional tyrosine motifs YXXØ are present in the C-terminal region. Each of these is involved in a different Golgi-to-vacuole targeting pathway: the tyrosine...
|
PMID: 20406419
PDF is available here.
PDF is available here.
Abstract
We focused on the 79 residue Sna2p protein. We found that Sna2p is localized in the vacuolar membrane. Directed mutagenesis showed that two functional tyrosine motifs YXXØ are present in the C-terminal region. Each of these is involved in a different Golgi-to-vacuole targeting pathway: the tyrosine...
|
PMID: 20406419
PDF is available here.
PDF is available here.
Abstract
We focused on the 79 residue Sna2p protein. We found that Sna2p is localized in the vacuolar membrane. Directed mutagenesis showed that two functional tyrosine motifs YXXØ are present in the C-terminal region. Each of these is involved in a different Golgi-to-vacuole targeting pathway: the tyrosine...
|
PMID: 20406419
PDF is available here.
PDF is available here.
Abstract
We focused on the 79 residue Sna2p protein. We found that Sna2p is localized in the vacuolar membrane. Directed mutagenesis showed that two functional tyrosine motifs YXXØ are present in the C-terminal region. Each of these is involved in a different Golgi-to-vacuole targeting pathway: the tyrosine...
|
PMID: 20406419
PDF is available here.
PDF is available here.
Abstract
We focused on the 79 residue Sna2p protein. We found that Sna2p is localized in the vacuolar membrane. Directed mutagenesis showed that two functional tyrosine motifs YXXØ are present in the C-terminal region. Each of these is involved in a different Golgi-to-vacuole targeting pathway: the tyrosine...
|
PMID: 20406419
PDF is available here.
PDF is available here.
Abstract
We focused on the 79 residue Sna2p protein. We found that Sna2p is localized in the vacuolar membrane. Directed mutagenesis showed that two functional tyrosine motifs YXXØ are present in the C-terminal region. Each of these is involved in a different Golgi-to-vacuole targeting pathway: the tyrosine...
|
PMID: 20406419
PDF is available here.
PDF is available here.