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Welcome to the Pubget Medical Subject Headings (MESH) browser. Click on a topic or subtopic below to explore related papers. In the gold box is the most recent paper about the current MESH term, and below are 20 related papers.
Gene Expression Regulation, Bacterial (0):
Recent article
Gene Expression Regulation, Bacterial
Abstract
We describe a synthetic genetic circuit that couples cell density and motility. This system enabled programmed Escherichia coli cells to form periodic stripes of high and low cell densities sequentially and autonomously. Theoretical and experimental analyses reveal that the spatial structure arises...
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PMID: 21998392
PDF is available here.
PDF is available here.
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Abstract
We previously demonstrated that S. pneumoniae cleaves sialic acid from human glycoconjugates to be used as a carbohydrate source. The satABC genes are required for growth and import of sialic acid. The satABC genes are predicted to encode components of an ABC transporter but not the ATPases essentia...
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PMID: 21825065
PDF is available here.
PDF is available here.
Abstract
These results suggest that CDT does not solely determine severity of infection and clinical outcome....
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PMID: 21851421
PDF is available here.
PDF is available here.
Proteomics (11)18 2011
Abstract
This review covers progress in proteome research on Mycoplasma pneumoniae made over the last 5 years. This bacterium is one of the smallest known self-replicating bacteria. With fewer than 700 proposed proteins, it is well suited to a comprehensive proteome analysis. While all of the...
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PMID: 21751371
PDF is available here.
PDF is available here.
Bing du xue bao = Chinese journal of virology / [bian ji, Bing du xue bao bian ji wei yuan hui] (27)5 2011
Abstract
To express and prepare polyclonal antibody of Human papillomavirus type 6b (HPV6b) E7 protein. a prokaryotic expression vector pGEX-4T-2/HPV6b E7 was constructed and GST-HPV6b E7 fusion protein was expressed as a soluble protein in E. coli. The expressed fusion protein was purified v...
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PMID: 21998951
PDF is available here.
PDF is available here.
Cell Host & Microbe (10)2 2011
Abstract
We used massively parallel cDNA sequencing (RNA-seq) techniques to quantitatively catalog the transcriptome of the cholera pathogen, Vibrio cholerae, derived from two animal models of infection. Transcripts elevated in infected rabbits and mice relative to laboratory media derive from the major know...
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PMID: 21843873
PDF is available here.
PDF is available here.
Xi bao yu fen zi mian yi xue za zhi = Chinese journal of cellular and molecular immunology (27)8 2011
Abstract
To study whether rSj26-Sj32 fusion protein can be used for diagnosis of chronic schistosomiasis japonicum.
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PMID: 21806877
PDF is available here.
PDF is available here.
Xi bao yu fen zi mian yi xue za zhi = Chinese journal of cellular and molecular immunology (27)8 2011
Abstract
To construct the prokaryotic expression vector of human histone-1, obtain the purified GST-H1 protein, and detect its activity.
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PMID: 21806878
PDF is available here.
PDF is available here.
Abstract
We show using a kinetic model that Hfq can efficiently and robustly achieve this difficult task by minimizing the sequestration of sRNAs and target mRNAs in Hfq complexes. This sequestration can be reduced by two non-mutually exclusive kinetic mechanisms. The first mechanism involves heterotropic co...
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PMID: 21876666
PDF is available here.
PDF is available here.
Abstract
We map the fitness costs of a key metabolic network, the lactose utilization pathway in Escherichia coli. We measure the growth of several regulatory lac operon mutants in different environments inducing expression of the lac genes. We find a strikingly nonlinear fitness landscape, which depends on...
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PMID: 21814515
PDF is available here.
PDF is available here.
PLoS Pathogens (7)7 2011
Abstract
We performed high throughput sequencing of cDNA generated from sRNA transcripts isolated from a strain ectopically expressing ToxT, the major transcriptional regulator within the virulence gene regulon. We compared this data set with ToxT binding sites determined by pulldown and deep sequencing to i...
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PMID: 21779167
PDF is available here.
PDF is available here.
Abstract
We have produced human CFTR in the prokaryotic expression host Lactococcus lactis. The full-length protein was detected in the membrane of the bacterium, but the yields were too low (< 0.1% of membrane proteins) for in vitro functional and structural characterization, and induction of the expression...
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PMID: 21742800
PDF is available here.
PDF is available here.
Abstract
We make use of the random partial least squares regression technique (r-PLS) to trace connections between co-expressed genes in Mycobacterium tuberculosis using data downloaded from public microarray databases. We generated the overall topology of a microbial co-expression network with the exact com...
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PMID: 21514402
PDF is available here.
PDF is available here.
Proteomics (11)12 2011
Abstract
Small-colony variants (SCVs) of Staphylococcus aureus represent a slow-growing subpopulation causing chronic and relapsing infections due to their physiological adaptation on an intracellular lifestyle. In this first proteomic study on physiological changes associated with a natural,...
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PMID: 21595038
PDF is available here.
PDF is available here.
Abstract
We show here that stochastic switching of a monostable circuit can mediate the adaptation of the engineered OSU12-hisC Escherichia coli strain to histidine starvation. In this strain, the hisC gene was deleted from the His operon and placed under the control of a monostable foreign promoter. In resp...
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PMID: 21613982
PDF is available here.
PDF is available here.
Xenobiotica (41)5 2011
Abstract
This chapter provides methods and insights into the use of antisense RNA as a molecular genetic tool. Posttranscriptional inhibition of specific gene expression can be achieved by antisense RNA fragments under control of a conditional promoter. Effective titration of gene expression can cause an app...
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PMID: 18392976
PDF is available here.
PDF is available here.
Xenobiotica (41)5 2011
Abstract
A method is described for generating conditional lethal mutations in essential genes in Escherichia coli. In this procedure, amber stop codons are introduced as "tagalong" mutations in the flanking DNA of a downstream antibiotic-resistance marker by lambda Red recombination. The marker is removed by...
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PMID: 18392977
PDF is available here.
PDF is available here.
Abstract
We have fused the promoterless lux operon luxCDABE to the promoter regions of Anabaena sp. PCC 7120 phoA genes putatively encoding alkaline phosphatases, phoA (all2843) and phoA-like (alr5291) and to the promoter region of one operon putatively encoding a high affinity phosphate transporter pst1 (al...
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PMID: 21533636
PDF is available here.
PDF is available here.
Abstract
New Microbiologica (34)2 2011
Abstract
We describe a simple method allowing RickA protein to be localized by immunofluorescence assay (IFA) and transmission electron microscopy (TEM). By using IFA we showed the global expression of surface protein RickA in R. conorii organisms. The TEM results showed that RickA is widely expressed over t...
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PMID: 21617834
PDF is available here.
PDF is available here.
Abstract
The cichlid oscar Astronotus ocellatus has worldwide commercial value in the pet fish industry because of its early maturation, relatively high fecundity, ability to identify its caretaker and also to alter colouration amongst conspecifics. Pathogenic strains of Aeromonas veronii res...
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PMID: 21553566
PDF is available here.
PDF is available here.
Abstract
The transcription factor SoxR, which serves as a cellular redox sensor, is a global activator of antioxidative stress in Escherichia coli. The granaticin biosynthetic gene orf20 from Streptomyces vietnamensis was found to be a soxR-like gene. This study was carried out towards understanding the physi...
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PMID: 21604555
PDF is available here.
PDF is available here.
Avian Diseases (55)1 2011
Abstract
As no data are available on the prevalence of cytolethal distending toxin (cdt) genes carried by Campylobacter spp. in laying hens, this study was conducted with the aim to evaluate the prevalence of both Campylobacter spp. and cdt genes in 1680 laying hens from four different farms. The samples were...
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PMID: 21500644
PDF is available here.
PDF is available here.
Abstract
Bacterial intergenic spacers are non-coding genomic regions enriched with cis-regulatory elements for gene expression. A population genetics approach was used to investigate the evolutionary force shaping the genetic diversity of intergenic spacers among 13 genomes of group A strepto...
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PMID: 21115137
PDF is available here.
PDF is available here.
Systems Biology, IET (5)2 2011
Abstract
Many methods had been developed on inferring transcriptional network from gene expression. However, it is still necessary to design new method that discloses more detailed and exact network information. Using network-assisted regression, the authors combined the averaged three-way mu...
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PMID: 21405197
PDF is available here.
PDF is available here.
Abstract
These results revealed pleiotropic roles of LuxS and AI-2 on A. pleuropneumoniae virulence traits....
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PMID: 21320583
PDF is available here.
PDF is available here.
Abstract
Moritella viscosa is considered to be the main aetiological agent of winter ulcer disease, primarily affecting farmed salmonid fish in cold marine waters. Transcription profiles of twelve M. viscosa genes, potentially involved in the pathogenesis, were studied during the course of an in vitro cell cu...
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PMID: 21334427
PDF is available here.
PDF is available here.
Abstract
We constructed a Salmonella mutant strain in which gidA was deleted. Deletion of gidA rendered Salmonella deficient in the invasion of intestinal epithelial cells, bacterial motility, intracellular survival, and induction of cytotoxicity in host cells. Deletion of gidA rendered the organism to displ...
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PMID: 21320585
PDF is available here.
PDF is available here.
Abstract
We demonstrated that loss of phoPQ from both strains leads to a defect in intracellular growth and/or survival within macrophages. These in vitro data would suggest that the phoPQ mutants would be attenuated in vivo. However, the LD(50) for the Y. pestis mutant did not differ from the calculated LD(...
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PMID: 21320584
PDF is available here.
PDF is available here.
Abstract
A major class of bacterial small RNAs (sRNAs), along with RNA-binding protein Hfq and endoribonuclease RNase E, acts on target mRNAs through base-pairing, leading to translational repression and rapid degradation of the mRNAs. In this issue of Genes & Development, Prévost and colleagues (pp. 385-396...
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PMID: 21325130
PDF is available here.
PDF is available here.
Abstract
To investigate the function of flavohaemoglobin (HMP) in Staphylococcus aureus RN6390 under the nitrification pressure, we constructed the hmp gene deletion mutant of RN6390 strain.
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PMID: 21574380
PDF is available here.
PDF is available here.
Abstract
Escherichia coli DH5α grows poorly in a chemically defined medium, but its acetate-tolerant mutant, DA19, grows fast and produces less acetate. To investigate the differences in metabolism between the two strains, the samples of continuous cultures at steady state were analyzed by t...
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PMID: 20700606
PDF is available here.
PDF is available here.
Abstract
We have isolated the GAPDH protein of Mycoplasma bovis and we are currently using this protein as a test antigen to develop a vaccine to protect feedlot animals from M. bovis-related diseases. As part of our vaccine studies, we are testing several novel immune modulators, some of which are host-defe...
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PMID: 21296650
PDF is available here.
PDF is available here.
Abstract
Pseudomonas aeruginosa produces multiple virulence factors that have been implicated in pathogenesis and quorum sensing. The aim of this study was to determine differences in the virulence factors of pigmented and non-pigmented P aeruginosa isolates.
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PMID: 21809707
PDF is available here.
PDF is available here.
Abstract
Advances in Genetics (74) 2011
Abstract
The cyanobacterium Synechococcus elongatus PCC 7942 has been used as the prokaryotic model system for the study of circadian rhythms for the past two decades. Its genetic malleability has been instrumental in the discovery of key input, oscillator, and output components and has also provided monument...
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PMID: 21924974
PDF is available here.
PDF is available here.
Abstract
We know little regarding Pel. In this study, we demonstrate that Pel can serve two functions in biofilms. Using a novel assay involving optical tweezers, we demonstrate that Pel is crucial for maintaining cell-to-cell interactions in a PA14 biofilm, serving as a primary structural scaffold for the c...
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PMID: 21298031
PDF is available here.
PDF is available here.
Abstract
These results suggest that transcriptional termination, or lack thereof, plays a role in rickettsial gene regulation....
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PMID: 21298070
PDF is available here.
PDF is available here.
Abstract
We employed the nematode Caenorhabditis elegans as an in vivo infection model. We demonstrate that AIEC strain LF82 forms a persistent infection in C. elegans, thereby reducing the host lifespan significantly. This host killing phenotype was associated with massive bacterial colonization of the nema...
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PMID: 21298102
PDF is available here.
PDF is available here.
Abstract
We also show that the biosynthesis of secondary metabolites, such as microcystins, aeruginosin and cyanopeptolin, occur essentially during the light period, suggesting that these metabolites may interact with the diurnal part of the central metabolism....
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PMID: 21283831
PDF is available here.
PDF is available here.
Abstract
We will discuss the contribution of 'omics' approaches to these discoveries as well as the possible impact that they are expected to have in the future....
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PMID: 21836626
PDF is available here.
PDF is available here.
Abstract
Here we present a Bayesian method that identifies which known transcriptional relationships in a regulatory network are consistent with a given body of static gene expression data by eliminating the non-relevant ones. The Partially Observed Bipartite Network (POBN) approach developed here is tested...
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PMID: 21619639
PDF is available here.
PDF is available here.
Abstract
The genome-scale metabolic model, RehMBEL1391, successfully represented metabolic characteristics of R. eutropha H16 at systems level. The reconstructed genome-scale metabolic model can be employed as an useful tool for understanding its metabolic capabilities, predicting its physiological consequen...
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PMID: 21711532
PDF is available here.
PDF is available here.
Abstract
Membrane proteins comprise an important class of molecules whose study is largely frustrated by several intrinsic constraints, such as their hydrophobicity and added requirements for correct folding. Additionally, the complexity of the cellular mechanisms that are required to insert membrane proteins...
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PMID: 21818275
PDF is available here.
PDF is available here.
Abstract
We show that in an exponentially growing Escherichia coli rpoZ mutant lacking the polymerase ω subunit, the impact of the Eσ(38) holoenzyme on transcription is enhanced in parallel with overall DNA relaxation. Conversely, overproduction of σ(70) in an rpoZ mutant increases both overall DNA superc...
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PMID: 21810966
PDF is available here.
PDF is available here.
Abstract
We have identified an additional kinase, CrdS, which directly regulates the phosphorylation state of CrdA. Both epistasis and in vitro phosphotransfer assays indicate that CrdS functions as part of the Che3 pathway and, in addition to CheA3, serves to regulate CrdA phosphorylation in M. xanthus. We...
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PMID: 21810965
PDF is available here.
PDF is available here.
Abstract
We report the regulatory response of C. crescentus to carbon starvation, based on combined high-throughput proteome and transcriptome analyses. Our results identify cell cycle changes in gene expression in response to carbon starvation that involve the prominent role of the FixK FNR/CAP family trans...
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PMID: 21494595
PDF is available here.
PDF is available here.
Abstract
We have used the Mammalian Glycan Array to determine the specific affinities of these proteins. This was correlated with binding protein expression induced by different prebiotics including HMO. Half of the F1SBPs in B. infantis were determined to bind mammalian oligosaccharides. Their affinities in...
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PMID: 21423604
PDF is available here.
PDF is available here.
Abstract
We explored the global transcriptional regulation by CovR in S. mutans. Comparison of the transcriptome profiles of the wild-type strain UA159 with its isogenic covR deleted strain IBS10 indicated that at least 128 genes (∼6.5% of the genome) were differentially regulated. Among these genes, 69 we...
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PMID: 21655290
PDF is available here.
PDF is available here.
Abstract
We previously demonstrated that, in response to B. pseudomallei infection of macrophage cell line RAW 264.7, a subset of bacteria co-localized with the autophagy marker protein, microtubule-associated protein light chain 3 (LC3), implicating autophagy in host cell defence against infection. Recent r...
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PMID: 21412437
PDF is available here.
PDF is available here.
Abstract
We have shown that WalKR acts as the master controller of peptidoglycan metabolism, yet none of the identified regulon genes explain its requirement for cell viability. Transmission electron micrographs revealed cell wall thickening and aberrant division septa in the absence of WalKR, suggesting its...
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PMID: 21386961
PDF is available here.
PDF is available here.
Abstract
We demonstrate that Hfq, an RNA-binding protein, which facilitates interactions between sRNAs and their mRNA targets, is critical for K. pneumoniae virulence. A K. pneumoniae mutant lacking hfq (Δhfq) failed to disseminate into extra-intestinal organs and was attenuated on induction of a systemic i...
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PMID: 21779404
PDF is available here.
PDF is available here.
Abstract
We have utilized sensitive proteomic approaches to examine the impact of diurnal rhythms on the protein complement in Cyanothece 51142. We found that 250 proteins accounting for ∼5% of the predicted ORFs from the Cyanothece 51142 genome and 20% of proteins detected under alternating light/dark con...
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PMID: 21364985
PDF is available here.
PDF is available here.
Abstract
These results provided a fundamental understanding of carbohydrate metabolism in alkaliphiles....
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PMID: 21253875
PDF is available here.
PDF is available here.
Gut Microbes (2)1 2011
Abstract
We recently described modulation in expression of BabA and related outer membrane proteins that occurs during colonization of experimental animals. Here we put these findings into a broader context, and speculate on their implications for the host-pathogen relationship.
© 2011 Lande...
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PMID: 21637017
PDF is available here.
PDF is available here.
Abstract
We describe methodology for studying the role of bacterial ribosome modification in the regulation of gene expression. Ribosomal components modification influences translation efficiencies of certain mRNAs. Proteome analysis allows us to identify cellular protein composition change caused by ribosom...
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PMID: 21460884
PDF is available here.
PDF is available here.
Journal of Biological Chemistry (285)52 2010
Abstract
We proposed that FtsY requires functional interaction with inner membrane lipids at a late stage of the signal recognition particle pathway. In addition, an essential lipid-binding α-helix was identified in FtsY of various origins. Theoretical considerations and in vitro studies have suggested that...
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PMID: 20956528
PDF is available here.
PDF is available here.
Biochimica et Biophysica Acta (1808)4 2011
Abstract
We thus hypothesized that fatty acid composition of Legionella's membrane might influence the sensitivity of the bacteria to warnericin RK. Relationship between sensitivity to the peptide and fatty acid composition was then followed in various conditions. Cells in stationary phase, which were alread...
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PMID: 21182824
PDF is available here.
PDF is available here.
Abstract
We review recent efforts that investigate and quantify the coupling between the circadian and cell cycle clocks in cyanobacteria as a model system. We discuss studies that quantify the coupling from a systems point of view in which the oscillators are described in abstract terms. We also emphasize r...
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PMID: 20947336
PDF is available here.
PDF is available here.
Trends in Microbiology (18)12 2010
Abstract
We examined earlier data indicating its role in pathogenesis. We conclude that although T6S can, in rare instances, directly influence interactions with higher organisms, the broader physiological significance of the system is likely to provide defense against simple eukaryotic cells and other bacte...
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PMID: 20961764
PDF is available here.
PDF is available here.