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Protein Modification, Translational (1):
Recent article
Protein Modification, Translational
Abstract
We review modern developments in genomics and systems biology that have revolutionized our understanding of the multiple means by which translation is regulated. We suggest new means to model the process of translation in a richer framework that will incorporate information about gene sequences, the...
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PMID: 21487400
PDF is available here.
PDF is available here.
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Abstract
Aims and background. Elevated TGF-BETA1 secretion and down-modulation of NKG2D underlies impaired NK cytotoxicity in cancer patients. However, the molecular mechanism of immunosuppression by TGF-BETA1 is not yet clarified.Methods. IL-2-activated human NK cells were cultured with TGF-BETA1. Protein le...
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PMID: 21789015
PDF is available here.
PDF is available here.
Biochemical Pharmacology (80)3 2010
Abstract
We have investigated two transcription factors known to be sensitive to electrophilic stress and redox perturbation, Nrf2 and NF-kappaB, in mouse liver cells. Cellular stress was induced by the probes: N-acetyl-p-benzoquinineimine (NAPQI), the reactive metabolite of acetaminophen; dinitrochlorobenze...
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PMID: 20416283
PDF is available here.
PDF is available here.
Abstract
We demonstrate significant in vitro activities and multifaceted specificities of Aspergillus protein arginine methyltransferases (PRMTs) and we provide evidence for a role of protein methylation in mechanisms of oxidative stress response. We have isolated all three Aspergillus PRMTs from fungal extr...
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PMID: 20338257
PDF is available here.
PDF is available here.
Abstract
We observed, however, that the protein downstream of 2A (no signal) was translocated into the endoplasmic reticulum (ER). We interpreted these data as a form of 'slipstream' translocation: downstream proteins, without signals, were translocated through a translocon pore already formed by the signal...
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PMID: 19946875
PDF is available here.
PDF is available here.
Abstract
We observed, however, that the protein downstream of 2A (no signal) was translocated into the endoplasmic reticulum (ER). We interpreted these data as a form of 'slipstream' translocation: downstream proteins, without signals, were translocated through a translocon pore already formed by the signal...
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PMID: 19946875
PDF is available here.
PDF is available here.
Abstract
We observed, however, that the protein downstream of 2A (no signal) was translocated into the endoplasmic reticulum (ER). We interpreted these data as a form of 'slipstream' translocation: downstream proteins, without signals, were translocated through a translocon pore already formed by the signal...
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PMID: 19946875
PDF is available here.
PDF is available here.
Abstract
We observed, however, that the protein downstream of 2A (no signal) was translocated into the endoplasmic reticulum (ER). We interpreted these data as a form of 'slipstream' translocation: downstream proteins, without signals, were translocated through a translocon pore already formed by the signal...
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PMID: 19946875
PDF is available here.
PDF is available here.
Abstract
We observed, however, that the protein downstream of 2A (no signal) was translocated into the endoplasmic reticulum (ER). We interpreted these data as a form of 'slipstream' translocation: downstream proteins, without signals, were translocated through a translocon pore already formed by the signal...
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PMID: 19946875
PDF is available here.
PDF is available here.
Analytical Biochemistry (394)2 2009
Abstract
We present a new method for tracking translation steps in cell-free protein synthesis using atomic force microscopy (AFM). The cell-free protein synthesis system is useful to track the inherent translational progress of a target gene, whereas conventional UV absorption measurement coupled with densi...
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PMID: 19643072
PDF is available here.
PDF is available here.
Abstract
BACKGROUND: Systemic lupus erythematosus (SLE) is a multi-system involvement autoimmune disease that commonly occurs in childbearing age women. During pregnancy and postpartum period, disease activity may be severe or unchanged. Disease flare during pregnancy consistently affects pregnancy outcome....
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PMID: 19253790
PDF is available here.
PDF is available here.
Journal of Biological Chemistry (283)51 2008
Abstract
We hypothesized that a mutual interdependency exists between MT1-MMP trafficking and glycosylation. Lectin precipitation, metabolic labeling, enzymatic deglycosylation, and site-directed mutagenesis studies demonstrate that the LL(572) motif in the cytoplasmic tail of MT1-MMP influences the composit...
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PMID: 18955496
PDF is available here.
PDF is available here.
Journal of Biological Chemistry (283)48 2008
Abstract
We show that expression of GnT-Vb promotes its dimerization and inhibits RPTP{beta} intrinsic phosphatase activity, resulting in higher levels of phosphorylated {beta}-catenin, suggesting a mechanism by which GnT-Vb glycosylation couples to changes in cell adhesion. GnT-Vb-mediated glycosylation of...
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PMID: 18838383
PDF is available here.
PDF is available here.
Experimental Cell Research (314)19 2008
Abstract
We have previously described differential effects of physiologic (5%) and pathologic (18%) cyclic stretch (CS) on agonist-induced pulmonary endothelial permeability. This study examined acute and chronic effects of CS on agonist-induced intracellular signaling and cell morphology in the human lung m...
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PMID: 18824167
PDF is available here.
PDF is available here.
Journal of Biological Chemistry (283)45 2008
Abstract
The crystal structure of glucooligosaccharide oxidase from Acremonium strictum was demonstrated to contain a bicovalent flavinylation, with the 6- and 8alpha-positions of the flavin isoalloxazine ring cross-linked to Cys(130) and His(70), respectively. The H70A and C130A single mutants still retain...
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PMID: 18768475
PDF is available here.
PDF is available here.
Journal of Biological Chemistry (283)39 2008
Abstract
We report that the sustained alpha4beta1 activation associated with macrophage differentiation results from expression of beta1 integrin subunits that lack alpha2-6-linked sialic acids, a carbohydrate modification added by the ST6Gal-I sialyltransferase. During differentiation of U937 monocytic cell...
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PMID: 18650447
PDF is available here.
PDF is available here.
Biological Chemistry (389)9 2008
Abstract
We describe a procedure for such coupling of functionally active ribosomes that permits single-molecule studies of protein synthesis. Oxidation with NaIO4 at the 3' end of 23S rRNA and subsequent reaction with a biotin hydrazide produces biotinylated 70S ribosomes, which can be immobilized on a stre...
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PMID: 18713011
PDF is available here.
PDF is available here.
Abstract
We investigated the internalization and recycling pathways followed by CD1a and the role of its CT. We found that CD1a can be internalized by a clathrin- and dynamin-independent pathway and that it follows a Rab22a- and ADP ribosylation factor (ARF)6-dependent recycling pathway, similar to other car...
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PMID: 18564371
PDF is available here.
PDF is available here.
Journal of Biological Chemistry (283)35 2008
Abstract
We have shown that apoSOD1 without disulfide is the most facile state for formation of amyloid-like fibrillar aggregates. fALS mutations impair either zinc binding, disulfide formation, or both, leading to accumulation of the aggregation-prone, apo, and disulfide-reduced SOD1. Moreover, we have foun...
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PMID: 18552350
PDF is available here.
PDF is available here.
Biophysical Journal (95)3 2008
Abstract
We have developed a gene sequence-specific mechanistic model for translation which accounts for all the elementary steps of translation elongation. Included in our model is the nonspecific binding of tRNAs to the ribosomal A site, and we find that the competitive, nonspecific binding of the tRNAs is...
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PMID: 18359800
PDF is available here.
PDF is available here.
Journal of Biological Chemistry (283)27 2008
Abstract
We postulated a different proteolytic process to be responsible. Here, we show that Kex1 protease is involved in the programmed cell death caused by defective N-glycosylation. Its disruption decreases caspase-like activity, production of reactive oxygen species, and fragmentation of mitochondria and...
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PMID: 18474590
PDF is available here.
PDF is available here.
Journal of Biological Chemistry (283)27 2008
Abstract
We demonstrate that O-mannosylation within the mucin domain of hDG occurs preferentially at Thr/Ser residues that are flanked by basic amino acids. Protein O-mannosylation is independent of a consensus sequence, but strictly dependent on a peptide region located upstream of the mucin domain. This pe...
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PMID: 18456664
PDF is available here.
PDF is available here.
Journal of Biological Chemistry (283)23 2008
Abstract
A gene encoding Trypanosoma brucei UDP-N-acetylglucosamine pyrophosphorylase was identified, and the recombinant protein was shown to have enzymatic activity. The parasite enzyme is unusual in having a strict substrate specificity for N-acetylglucosamine 1-phosphate and in being located inside a per...
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PMID: 18381290
PDF is available here.
PDF is available here.
Glycobiology (18)6 2008
Abstract
We studied PSGL-1 glycosylation in the murine cell line WEHI-3 using metabolic-radiolabeling with (3)H-monosaccharide precursors to detect low-abundance O-glycan structures. We report that PSGL-1 from WEHI-3 cells expresses a di-sialylated core 2 O-glycan containing the SLe(x) antigen. This fucosyla...
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PMID: 18310305
PDF is available here.
PDF is available here.
Abstract
Systems biology studies have revealed transcriptional networks and proteomic signatures critical for embryonic stem cell (ESC) function. In this issue of Cell Stem Cell, Sampath et al. (2008) demonstrate that translation is also differentially controlled in undifferentiated versus differentiated ESC...
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PMID: 18462690
PDF is available here.
PDF is available here.
Abstract
We comprehensively profiled gene expression during differentiation of murine embryonic stem cells (ESCs) into embryoid bodies by integrating transcriptome analysis with global assessment of ribosome loading. While protein synthesis was parsimonious during self-renewal, differentiation induced an ana...
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PMID: 18462695
PDF is available here.
PDF is available here.
Abstract
We recently found that CLP expression up-regulates Myo10, leading to increased Myo10-dependent cell motility and filopodial extension [R.D. Bennett, et al., J. Biol. Chem. 282 (2007) 3205-3212]. CLP-dependent Myo10 up-regulation occurs without increase in Myo10 mRNA. We followed Myo10 degradation in...
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PMID: 18295593
PDF is available here.
PDF is available here.
Journal of Biological Chemistry (283)15 2008
Abstract
We found that Rem is targeted to the ER, where the C-terminal portion is translocated and glycosylated. The signal sequence is cleaved off and accumulates in nucleoli. In a cell-free in vitro system, the generation of the Rem signal peptide depends on the presence of microsomal membranes. In vitro a...
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PMID: 18270201
PDF is available here.
PDF is available here.
Physiological Reviews (88)2 2008
Abstract
We present well-known and recently identified proteins that sense and decode the calcium signal and are key elements in the nucleus to regulate the activity of various transcriptional networks. When possible, the review also presents in vivo models in which the genes encoding these calcium sensors-t...
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PMID: 18391169
PDF is available here.
PDF is available here.
Abstract
Journal of Biological Chemistry (283)12 2008
Abstract
We and others found this gene to encode a beta1,3-glucosyltransferase involved in the synthesis of the disaccharide Glc-beta1,3-Fuc-Omicron-that occurs on thrombospondin type 1 repeats of many biologically important proteins. No functional tests have been performed to date on the presumed glycosylat...
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PMID: 18199743
PDF is available here.
PDF is available here.
Journal of Biological Chemistry (283)12 2008
Abstract
We have investigated the effects of N-glycosylation on the activity and pharmacokinetics of a small bispecific single-chain diabody (scDb CEACD3) developed for the retargeting of cytotoxic T cells to CEA-expressing tumor cells. We could show that the introduction of N-glycosylation sequons into the...
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PMID: 18211902
PDF is available here.
PDF is available here.
Aging Cell (7)2 2008
Abstract
We describe a novel mechanism for regulating mitochondrial ROS production and lifespan in C. elegans: progressive mitochondrial protein modification by the glycolysis-derived dicarbonyl metabolite methylglyoxal (MG). We demonstrate that the activity of glyoxalase-1, an enzyme detoxifying MG, is mark...
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PMID: 18221415
PDF is available here.
PDF is available here.
Abstract
Our results provide preclinical evidence that both drugs stimulate chloride transport activity of F508del-CFTR protein....
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PMID: 18006891
PDF is available here.
PDF is available here.
Abstract
Abstract
The endoplasmic reticulum (ER) is a key organelle of the secretion pathway involved in the synthesis of both proteins and lipids destined for multiple sites within and without the cell. The ER functions to both co- and post-translationally modify newly synthesized proteins and lipids and sort them f...
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PMID: 18172663
PDF is available here.
PDF is available here.
Abstract
Abstract
We report here that two recently cloned betaGTs, betaGT3 and betaGT4, that are able to transfer GalNAc to GlcNAc in beta1,4-linkage display the necessary glycoprotein specificity in vivo. Both betaGTs transfer GalNAc to N-linked oligosaccharides on the luteinizing hormone alpha subunit and CA6 but n...
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PMID: 18048353
PDF is available here.
PDF is available here.
Abstract
These results suggest epithelial cells can regulate tightness of cell junctions via remodeling of N-glycans, including those linked to the Na,K-ATPase beta(1)-subunit....
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PMID: 18025087
PDF is available here.
PDF is available here.
Inefficient glycosylation leads to high steady-state levels of actively degrading cardiac triadin-1.
Abstract
We overexpressed triadin-1 as a series of glycoform variants in non-muscle cell lines and neonatal heart cells using plasmid and adenoviral vectors. We showed that the characteristic incomplete glycosylation stemmed from properties of the glycosylation sequence that are conserved among triadin splic...
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PMID: 18025088
PDF is available here.
PDF is available here.
Abstract
Here we show that the Nodulin26-like Intrinsic Proteins (NIPs) AtNIP5;1 and AtNIP6;1 from Arabidopsis thaliana, OsNIP2;1 and OsNIP3;2 from Oryza sativa, and LjNIP5;1 and LjNIP6;1 from Lotus japonicus are bi-directional As(III) channels. Expression of these NIPs sensitized yeast cells to As(III) and...
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PMID: 18544156
PDF is available here.
PDF is available here.
Abstract
Abstract
We have conducted experiments aimed to modify proteins of known structure using TGase and, surprisingly, we were able to obtain site-specific modification or PEGylation of protein-bound Gln residue(s) in the protein substrates. For example, in apomyoglobin (apoMb, myoglobin devoid of heme) only Gln9...
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PMID: 17916398
PDF is available here.
PDF is available here.
Biological Chemistry (389)1 2008
Abstract
The nightly bioluminescence of the dinoflagellate Gonyaulax is a circadian rhythm caused by the presence in cells of specialized bioluminescent organelles, termed scintillons, containing the reaction catalyst luciferase, the substrate luciferin and a luciferin-binding protein (LBP). LBP levels incre...
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PMID: 18095865
PDF is available here.
PDF is available here.
ChemBioChem (8)18 2007
Abstract
Cancer Letters (257)2 2007
Abstract
We review and discuss the diverse roles of involved modulators in both HIFs stability and degradation, describe the potential link between the two molecular scenarios....
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PMID: 17920193
PDF is available here.
PDF is available here.
Abstract
We analyzed structural and functional properties of the human estrogen receptor alpha synthesized by different eukaryotic translation systems. A minimum of three conformations of the receptor were detected using limited proteolysis and a sterol ligand-binding assay. The receptor in vitro translated...
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PMID: 17900518
PDF is available here.
PDF is available here.
Journal of Mass Spectrometry (42)11 2007
Abstract
We identified the major enzymes involved in both cytosolic and hydrogenosomal metabolic pathways, as well as putative protein targets for new drug design. In addition, this analysis allows validation of previous gene predictions confirming the expression of 15 hypothetical proteins. Finally, the fin...
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PMID: 17960578
PDF is available here.
PDF is available here.
Journal of Biological Chemistry (282)42 2007
Abstract
We identified a new extracellular protein, TM14, by differential hybridization using mouse tooth germ cDNA microarrays. TM14 cDNA encodes 440 amino acids containing a signal peptide. The protein contains 3 EGF modules at the center, a C-terminal domain homologous to the fibulin module, and a unique...
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PMID: 17699513
PDF is available here.
PDF is available here.
Biochimica et Biophysica Acta (1770)10 2007
Abstract
We demonstrate in the physiologically relevant mouse primary myoblast system that the expression of Pax3 decreases nearly 95% during the first 24 h of myogenic differentiation. In contrast, there is an aberrant persistence of expression of Pax3-FKHR during this same time period. These differences in...
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PMID: 17698292
PDF is available here.
PDF is available here.
Abstract
Intervention of oral supplementation with alpha-tocopherol did not result in changes in circulating oxidative protein modifications. A larger study may be required to show an effect in this clinical setting....
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PMID: 17660032
PDF is available here.
PDF is available here.
DNA and Cell Biology (26)8 2007
Abstract
We have analyzed the behavior of the protein during unperturbed DNA replication, in different replication and checkpoint mutant backgrounds and in response to DNA-damaging agents. In an unperturbed cell cycle, Rad4 is chromatin bound and the mobility of the protein is not altered. Rad4 protein level...
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PMID: 17688408
PDF is available here.
PDF is available here.
Journal of Biological Chemistry (282)28 2007
Abstract
We show that in mammalian cells the N15Q mutant protein trafficked to the plasma membrane and mediated copper uptake at 75% of the rate of wild-type hCTR1. We demonstrate that the extracellular amino terminus of hCTR1 also contains O-linked polysaccharides. Glycosidase treatment that removed O-linke...
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PMID: 17525160
PDF is available here.
PDF is available here.
Journal of Biological Chemistry (282)28 2007
Abstract
We investigate this hypothesis and show the following: 1) protein O-fucosyltransferase 1 is indeed the enzyme that adds O-fucose to Cripto; 2) Pofut1(-/-) embryonic stem cells behave the same as Pofut1(+/+) embryonic stem cells in a Nodal signaling assay; 3) Pofut1(-/-) and Pofut1(+/+) embryoid bodi...
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PMID: 17504756
PDF is available here.
PDF is available here.
Abstract
The tissue-specific changes in protein synthesis were tracked in relation to the seasonal metabolic depression in cunner (Tautogolabrus adsperus). In vivo protein synthesis rate and total RNA content were determined in liver, white muscle, brain, heart, and gill during periods of normal activity bef...
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PMID: 17379844
PDF is available here.
PDF is available here.
Abstract
Abstract
Secretory proteins unable to assemble into their native states in the endoplasmic reticulum (ER) are transported back or "retrotranslocated" into the cytosol for ER-associated degradation (ERAD). To examine the roles of different components in ERAD, one fluorescence-labeled ERAD substrate was encaps...
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PMID: 17540174
PDF is available here.
PDF is available here.
Analyst (Cambridge UK) (132)6 2007
Abstract
We present a brief overview of current strategies employed for top-down protein characterization and discuss the key technical challenges and solutions associated with their implementation on a range of mass spectrometry instrument platforms. For more specific details regarding the individual strate...
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PMID: 17525804
PDF is available here.
PDF is available here.
Abstract
The Notch signaling pathway is involved in a wide variety of highly conserved developmental processes in mammals. Importantly, mutations of the Notch protein and components of its signaling pathway have been implicated in an array of human diseases (T-cell leukemia and other cancers, Multiple Sclero...
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PMID: 17584081
PDF is available here.
PDF is available here.
Abstract
I defects create unoccupied glycosylation sites, while Type II defects give fully occupied sites with abnormally processed glycans. These types are expected to be mutually exclusive, but a group of patients is now emerging who have variable coagulopathy and hypoglycemia together with a combination o...
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PMID: 17584079
PDF is available here.
PDF is available here.