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Welcome to the Pubget Medical Subject Headings (MESH) browser. Click on a topic or subtopic below to explore related papers. In the gold box is the most recent paper about the current MESH term, and below are 20 related papers.
Gene Amplification (0):
Recent article
Gene Amplification
Abstract
We investigated the clinical relevance of β-arrestin-1 in breast cancer and elucidated a potential link between β-arrestin-1 expression and CCND1 amplification. β-Arrestin-1 protein expression was evaluated in two breast cancer patient cohorts, comprising 179 patients (cohort I) and 500 patients...
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PMID: 21497294
PDF is available here.
PDF is available here.
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Oral Oncology (47)6 2011
Abstract
We examined retrospectively the gene copy number of EGFR by FISH and the protein expression by immunohistochemistry using different epitope-specific antibodies in paraffin-embedded primary tumors of five different regions, from 71 HNSCC patients who had not been treated with anti-EGFR therapy. In se...
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PMID: 21498106
PDF is available here.
PDF is available here.
médecine/sciences (27)4 2011
Abstract
Nature Medicine (17)4 2011
Abstract
Abstract
We enriched amoeba cultures from sediments from four sites in the New England estuarine system of Mt. Hope Bay, Massachusetts and from sediments from six sites in the Great Salt Lake, Utah. Cultures of amoebae were enriched using both minimal- and non-nutrient agar plates, made with fresh water, bra...
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PMID: 21301113
PDF is available here.
PDF is available here.
Abstract
Variant alleles of vitamin K epoxide reductase complex subunit 1 gene (VKORC1), the target molecule of vitamin K antagonists, and of cytochrome P450 (CYP) 2C9, an enzyme involved in coumarin metabolism, affect the anticoagulant response of coumarins, which have a narrow therapeutic window. Genotyping...
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PMID: 21469766
PDF is available here.
PDF is available here.
Abstract
MYCN amplification prevents cellular differentiation and promotes mitotic and karyorrhectic activities in neuroblastomas. Hence, MYCN-amplified tumors typically show an appearance of neuroblastoma of either an undifferentiated or a poorly differentiated subtype with a high mitosis-karyorrhexis index....
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PMID: 21288077
PDF is available here.
PDF is available here.
Fungal Biology (115)4-5 2011
Abstract
A new method based on the analysis of mitochondrial intergenic regions characterized by intraspecific variation in DNA sequences was developed and applied to the study of the plant pathogen Phytophthora nicotianae. Two regions flanked by genes trnY and rns and trnW and cox2 were iden...
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PMID: 21530925
PDF is available here.
PDF is available here.
Abstract
We suggest that ITS sequences would be more suitable to serve as markers for authentication of Herba Leonuri than matK does....
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PMID: 21476215
PDF is available here.
PDF is available here.
Clinical Cancer Research (17)6 2011
Abstract
I lung adenocarcinoma (ADC), a considerable proportion of the patients will relapse within 5 years and show poor prognosis. The purpose of this study was to identify genetic alterations that define prognosis of patients with early-stage lung ADC....
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PMID: 21148746
PDF is available here.
PDF is available here.
Abstract
The human epidermal growth factor receptor-2 gene (HER-2) encodes for a membrane-bound tyrosine kinase (Her-2), which is overexpressed in various human cancers. Her-2-targeted therapy has recently been shown to be beneficial for patients with advanced gastric cancer. Her-2 protein expression was inv...
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PMID: 21107094
PDF is available here.
PDF is available here.
Bulletin du cancer (97)11 2010
Abstract
The high heterogeneity of clinical, histological, biological and genetic features in breast cancer is due in part to the extreme molecular complexity of these tumors. This review article presents the major technological advances of the past ten years, in particular the development of microarray appr...
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PMID: 21051317
PDF is available here.
PDF is available here.
Bulletin du cancer (97)11 2010
Abstract
Numerous molecular abnormalities have been described in lymphomas. They are of diagnostic and prognostic value and are taken into account for the WHO classification of these tumors. They also shed some light on the underlying molecular mechanisms involved in lymphomas. Overall, four types of molecul...
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PMID: 21084243
PDF is available here.
PDF is available here.
Bulletin du cancer (97)11 2010
Abstract
Sarcomas represent a heterogeneous group of tumors with a complex and poorly reproducible classification. However, in the last ten years, several specific genetic alterations have been described allowing a molecular classification with: 1) sarcomas with a specific translocation which can be used as...
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PMID: 21084242
PDF is available here.
PDF is available here.
Modern Pathology (23)10 2010
Abstract
We sought to analyze whether a distinctive genetic signature could be characterized that would reliably predict the treatment outcome. The ability to predict who will respond and who will become refractory to this agent will allow for improved, rational clinical management of these patients and furt...
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PMID: 20657551
PDF is available here.
PDF is available here.
Anticancer Research (30)10 2010
Abstract
Splice variants exist for both alpha and beta oestrogen receptors (ERs). Oestrogen function results from a balance between the wild-type ERs (wt) and their variants.
Forty formalin-fixed paraffin-embedded breast cancer samples were analysed by real-time PCR using ERα primer sets detecting wt and ex...
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PMID: 21036734
PDF is available here.
PDF is available here.
Abstract
Our experiments demonstrated that FISH was more sensitive than Southern blot at demonstrating low levels of gene amplification and, additionally, permitted assessment of the distribution of cyclin D1 gene among chromosomes....
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PMID: 19967453
PDF is available here.
PDF is available here.
Anticancer Research (30)10 2010
Abstract
Amplification of the HER-2/neu oncogene and concomitant over-expression of its protein are detected in approximately 18% of invasive ductal carcinoma of the breast and is associated with poor prognosis. This study tested the use of fluorescence in situ hybridization (FISH) and immunohistochemistry (...
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PMID: 21036723
PDF is available here.
PDF is available here.
Abstract
These results provide the first molecular evidence showing infection of dogs with B. gibsoni from Taiwan. On the other hand, a phylogenetic analysis based on the deduced amino acid sequence of the TRAP gene demonstrated that the Taiwanese isolates were closely related to strains previously identifie...
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PMID: 20519852
PDF is available here.
PDF is available here.
Abstract
We noted chromosomal amplification of Gli2, a downstream effector of Hh signaling, and, more rarely, point mutations in Smo that led to reactivated Hh signaling and restored tumor growth. Analysis of pathway gene expression signatures also, unexpectedly, identified up-regulation of phosphatidylinosi...
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PMID: 20881279
PDF is available here.
PDF is available here.
Abstract
We will summarize the current scientific knowledge of known target genes within 1q21 amplicon and the precise oncogenic mechanisms of CHD1L will be discussed in detail....
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PMID: 20676120
PDF is available here.
PDF is available here.
Journal of Pathology (222)1 2010
Abstract
Our data provide evidence that STK15 is oncogenic for OSCC and that its nuclear expression is a predictor of clinical behaviour. Knockdown of STK15 could be a potential therapeutic option in OSCC and other tumours....
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PMID: 20597069
PDF is available here.
PDF is available here.
Abstract
We first investigated the unique histogenesis of the basal/HER2+ phenotype in breast carcinomas. The presence of basal CK5/CK6 cytokeratin expression in HER2+ tumors revealed a significant overlap in the histological features of HER2+/CK5/6+ and basal-like breast carcinomas. Basal/HER2+ tumors were...
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PMID: 20664936
PDF is available here.
PDF is available here.
Abstract
We constructed and characterized a mutant virus (Δ41). In the absence of vhs activity, Δ41 mutant is highly attenuated in mice model and the lethality is correlated with the virus dissemination in neural tissues. As with herpes simplex virus type 1 (HSV-1), the prototype virus of alphaherpesvirus,...
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PMID: 20448414
PDF is available here.
PDF is available here.
Abstract
We evaluated antigen detection kits for human rotavirus with regard to their usefulness for diagnosing equine rotavirus infection. Limiting dilution analyses showed that of the seven kits investigated the Dipstick `Eiken' Rota (Dipstick) had the highest sensitivity to two serotypes of equine rotavir...
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PMID: 20453452
PDF is available here.
PDF is available here.
Abstract
The PCR assay detected viral DNA to a level of 1,200 copies/mL of whole blood. It was used to detect EEHV1 in trunk secretions of 3 of the 5 elephants surveyed during the 15-week period. Viral gene subtyping analysis identified 2 distinct elephant herpesviruses, 1 of which was identical to the virus...
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PMID: 20673092
PDF is available here.
PDF is available here.
Abstract
These results demonstrate that multiple copies of the pfmdr1-containing amplicon in AL-resistant parasites are unstable when drug pressure is withdrawn and have practical implications for the maintenance and spread of parasites resistant to artemisinin derivatives....
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PMID: 20421397
PDF is available here.
PDF is available here.
Toxicology (275)1-3 2010
Abstract
We investigated the centrosome abnormalities induced by diethyl sulfate (DES) in Chinese hamster lung (CHL) fibroblasts and the underlying molecular mechanisms. Our results showed that DES exposure at 0.3, 1 and 3mM for 48 h caused centrosome amplification in a dose dependent manner. This effect was...
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PMID: 20566339
PDF is available here.
PDF is available here.
Abstract
Our findings demonstrate that EGCG is effective for growth inhibition of a chondrosarcoma cell lines in vitro, and suggest that EGCG may be a new therapeutic option for patients with chondrosarcoma....
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PMID: 20127255
PDF is available here.
PDF is available here.
Abstract
Most H. pylori strains had a dupA allele that was longer (1884 bp; dupA1) than previously described dupA alleles, although some had truncated versions (dupA2). Unlike the best-characterized H. pylori virulence determinant, the cag pathogenicity island (cag PaI), neither dupA type induced release of...
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PMID: 20533870
PDF is available here.
PDF is available here.
ATM-deficient thymic lymphoma is associated with aberrant tcrd rearrangement and gene amplification.
Abstract
We now show that, in addition to the known chromosome 14 translocation, ATM-deficient mouse thymic lymphomas routinely contain a centromeric fragment of chromosome 14 that spans up to the 5' boundary of the Tcra/d locus, at which position a 500-kb or larger region centromeric to Tcra/d is routinely...
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PMID: 20566716
PDF is available here.
PDF is available here.
Abstract
This study revealed that fluorescence in situ hybridization resulted in a high concordance of 83.3% between the human epidermal growth factor receptor 2 manifestation (fluorescence in situ hybridization-positive status) in primary tumors and that in metastatic lymph nodes, demonstrating that it is a...
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PMID: 20202990
PDF is available here.
PDF is available here.
Abstract
Clinical Cancer Research (16)13 2010
Abstract
We used immunohistochemistry to examine recently developed antibodies specific to major hotspot mutations of L858R and DEL E746-A750.
We used five series of lung cancers: 47 non-small cell lung cancers (NSCLC) to evaluate various types of EGFR mutations, a consecutive series of 238 N...
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PMID: 20570926
PDF is available here.
PDF is available here.
Clinical Cancer Research (16)13 2010
Abstract
We can attempt detailed characterization of high-grade gliomas in children, that have until recently been lacking. Toward this end, we sought to validate and extend investigations of the differences between pediatric and adult tumors.
We carried out copy number profiling by array com...
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PMID: 20570930
PDF is available here.
PDF is available here.
Gene Therapy (17)7 2010
Abstract
We have developed a bidirectional plasmid vector, which uses a two-step transcriptional amplification (TSTA) strategy to enhance the expression of two optical reporter genes, firefly luciferase (fluc) and Renilla luciferase (hrluc), driven by the cardiac troponin T (cTnT) promoter. The vector was ch...
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PMID: 20237511
PDF is available here.
PDF is available here.
Abstract
We investigated whether de novo refractoriness to the anti-HER2 monoclonal antibody trastuzumab (Tzb; Herceptin) may relate to the dynamic regulation of the mesenchymal CD44(pos)/CD24(neg/low) phenotype in HER2-positive BC. We observed that the subpopulation of Tzb-refractory JIMT-1 BC cells exhibit...
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PMID: 20470755
PDF is available here.
PDF is available here.
Journal of Clinical Oncology (28)18 2010
Abstract
From March 2007 to April 2008, we enrolled 95 patients. Median age was 46 years (range, 28 to 73 years). At a median follow-up of 22 months, 92 were evaluable. Of the 92 patients, 41 patients (45%) withdrew for PTL-specific toxicities. Overall, 40 (43%) of 92 patients had lapatinib dose reductions,...
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PMID: 20479410
PDF is available here.
PDF is available here.
Abstract
In vitro Th1-polarized CD4 T cells did not express ST2L; however, most CD4 T cells became ST2L on repeated stimulation under Th2-polarizing conditions. Similarly, we found that IL-33 was able to enhance the expression of Th2-associated cytokines (IL-5 and IL-13) but not interferon (IFN)-gamma. Treat...
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PMID: 20220570
PDF is available here.
PDF is available here.
Abstract
Campylobacteriosis is a zoonosis that occurs worldwide. Infection with Campylobacter fetus (C. fetus) causes infertility and abortion in sheep and cattle. The current study focuses on the SapA gene of C. fetus that encodes surface array proteins and plays an important role in the virulence of C. fet...
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PMID: 20035963
PDF is available here.
PDF is available here.
Abstract
It has been proposed that regions of microhomology in the human genome could facilitate genomic rearrangements, copy number transitions, and rapid genomic change during tumor progression. To investigate this idea, this study examines the role of repetitive sequence elements, and corresponding synten...
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PMID: 20453501
PDF is available here.
PDF is available here.
Experimental Gerontology (45)6 2010
Abstract
We analyzed the phenotype of spinal curvature and expression of genes related to the anti-oxidant defense system and myogenesis in muscle of 8 and 30 months old GH-transgenic males. Gene expression analyses revealed that both superoxide dismutase isoforms were down-regulated only in 30 months old an...
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PMID: 20338232
PDF is available here.
PDF is available here.
Abstract
To identify a rapid method for extracting a large amount of DNA from fungi associated with food hygiene, extraction methods were compared using fungal pellets formed rapidly in liquid media. Combinations of physical and chemical methods or commercial kits were evaluated with 3 species of yeast, 10 s...
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PMID: 20537263
PDF is available here.
PDF is available here.
Abstract
These results demonstrate that extraction of DNA from processed animal protein is different for pure ingredients and fortified animal feeds. These results indicate that a method specifically developed using just animal-derived meat and bone meal may not yield a functional assay when used to detect a...
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PMID: 20537265
PDF is available here.
PDF is available here.
Abstract
We have, however, observed rare cases of PL, which arose in association with WDL and have studied these cases to define their clinicopathologic features and their nosologic relationship to other forms of liposarcoma. Cases were retrieved from our consultation archives and from review of cases treate...
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PMID: 20431480
PDF is available here.
PDF is available here.
Abstract
We tried to isolate Escherichia coli O157:H7 from fecal samples collected from dogs and cats. In results, E. coli O157:H7 was isolated from 1 out of 614 samples (0.16%). This isolate produced Stx1 and Stx2 and was isolated from a dog kept by a human patient infected with E. coli O157:H7. Excluding i...
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PMID: 20124764
PDF is available here.
PDF is available here.
Abstract
These results indicate that the RT-LAMP assay is specific for equine rotavirus and more sensitive than semi-nested RT-PCR. Because it is easy to manipulate without the need for a thermal cycler or gel electrophoresis, the RT-LAMP assay should be applicable to diagnosis of equine rotavirus infections...
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PMID: 20160420
PDF is available here.
PDF is available here.
Abstract
We found p53 to be largely localized in the host cell cytoplasm and associated with the parasite membrane of isolated schizonts. Curing infected cells of the parasite with the theilericidal drug buparvaquone resulted in a time-dependent translocation of p53 into the host cell nucleus and the upregul...
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PMID: 20208567
PDF is available here.
PDF is available here.
Bulletin du cancer (97)6 2010
Abstract
I and II trials demonstrated the antitumor activity of anti-IGF1R antibodies in Ewing, whose fusion gene downregulates IGFBP3. Inhibitors of MDM2 are in the course of clinical evaluation in liposarcomas. Inhibitors of mTOR (sirolimus, temsirolimus) demonstrated an antitumoral activity in the PEComas...
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PMID: 20497911
PDF is available here.
PDF is available here.
Abstract
We demonstrate here that EGFR activation by either mutation or ligand binding increases MET gene expression and protein levels. Our analysis of 202 NSCLC patient specimens was consistent with these observations: levels of MET were significantly higher in NSCLC with EGFR mutations than in NSCLC with...
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PMID: 20154724
PDF is available here.
PDF is available here.
Comptes Rendus Biologies (333)5 2010
Abstract
East Antarctic octopods were identified by sequencing mtCOI and using four analytical approaches: Neighbor-joining by Kimura-2-Parameter-based distances, character-based, BLAST, and Bayesian Inference of Phylogeny. Although the distance-based analytical approaches identified a high p...
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PMID: 20451881
PDF is available here.
PDF is available here.
Abstract
Immunohistochemistry, Western blot and real-time RT-PCR showed that RsTbetaRII-IFN-gamma treatment significantly inhibited liver expression of collagen III, alpha-SMA, TGF-beta1 and TGF-betaRII at both protein and mRNA levels. Histopathological analysis also showed that the enhanced anti-fibrotic ef...
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PMID: 20554496
PDF is available here.
PDF is available here.
Journal of Dairy Science (93)4 2010
Abstract
The effect of prolactin (PRL), beta-lactoglobulin (beta-LG), and kappa-casein (CSN3) on milk yield was estimated in an East Friesian dairy sheep population from Old Chatham Sheepherding Company, New York. Genotypes were determined by PCR amplification followed by digestion with HaeIII and RsaI for P...
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PMID: 20338452
PDF is available here.
PDF is available here.
Abstract
Antifolates, such as methotrexate (MTX), are the treatment of choice for numerous cancers. MTX inhibits dihydrofolate reductase (DHFR), which is essential for cell growth and proliferation. Mammalian cells can acquire resistance to antifolate treatment through a variety of mechanisms but decreased a...
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PMID: 19337845
PDF is available here.
PDF is available here.
Abstract
A novel universal real-time PCR, consisting of newly designed oligonucleotide subsets, was designed for a bacterial housekeeping gene encoding the peptide elongation factor Tu. Specificity and universality were confirmed in 66 bacterial strains, including 51 genera and 63 species. The amplification...
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PMID: 20377955
PDF is available here.
PDF is available here.
Genome Research (20)4 2010
Abstract
We developed a systematic approach to characterize the spectrum of cancer-associated mRNA alterations through integration of transcriptomic and structural genomic data, and we applied this approach to generate new insights into melanoma biology. Using paired-end massively parallel sequencing of cDNA...
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PMID: 20179022
PDF is available here.
PDF is available here.
Clinical Cancer Research (16)7 2010
Abstract
We hypothesized that additional drivers of tumor behavior would be contained within areas of consistent genomic copy number change, especially those associated with the WT risk groups defined by the International Society of Paediatric Oncology (SIOP)....
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PMID: 20332316
PDF is available here.
PDF is available here.
Abstract
These results indicate that the developed RT-LAMP assay with uniquely designed primers is potentially useful in comprehensive avian influenza surveillance....
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PMID: 20032626
PDF is available here.
PDF is available here.
Methods in Molecular Biology (630)630 2010
Abstract
We have piloted the use of real-time PCR measurement of specific prognostic genes, so called "Indicator" genes, in globally amplified polyA cDNA for this purpose. Poly(A) PCR coordinately amplifies cDNA copies of all polyadenylated mRNAs, thereby generating a PCR product (polyA cDNA) whose compositi...
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PMID: 20300988
PDF is available here.
PDF is available here.
Abstract
We performed an array-based comparative genomic hybridization survey of 128 non-small-cell lung cancer cell lines and tumors. Prominent among our findings, we identified recurrent high-level amplification at cytoband 22q11.21 in 3% of lung cancer specimens, with another 11% of specimens exhibiting l...
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PMID: 19966867
PDF is available here.
PDF is available here.