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Welcome to the Pubget Medical Subject Headings (MESH) browser. Click on a topic or subtopic below to explore related papers. In the gold box is the most recent paper about the current MESH term, and below are 20 related papers.
Mutagenesis (12):
Recent article
Mutagenesis
Journal of Bacteriology (192)14 2010
Abstract
We investigated the regulation of several Sodalis genes that encode putative iron acquisition systems. These genes, SG1538 (hemT) and SG1516 (sitA), are homologous to genes encoding periplasmic heme and iron/manganese transporters, respectively. hemT promoter- and sitA promoter-gfp fusions were cons...
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PMID: 20494987
PDF is available here.
PDF is available here.
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Journal of Bacteriology (192)14 2010
Abstract
We determined that the transcription of the auxiliary, antibiotic-resistant tryptophanyl-tRNA synthetase gene (trpRS1) in Streptomyces coelicolor is regulated by a ribosome-mediated attenuator in the 5' leader of its mRNA region. This regulatory element controls gene transcription in response to the...
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PMID: 20453096
PDF is available here.
PDF is available here.
Journal of Bacteriology (192)13 2010
Abstract
I mutants were inactive, resulting in nonregulated LPS Oag chains, while classes II and III conferred shorter LPS Oag chains of 2 to 10 and 8 to 14 RUs, respectively. Class IV mutants retained near-wild-type function, and class V mutants increased the LPS Oag chain length to 16 to 25 RUs. In vivo fo...
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PMID: 20453100
PDF is available here.
PDF is available here.
Journal of Bacteriology (192)13 2010
Abstract
We examined how defects in a transcriptional factor, previously reported to alter the acquisition of adaptive mutations, affected mutation levels in a gene under selection. The acquisition of mutations was directly correlated to the level of transcription of a defective leuC allele placed under sele...
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PMID: 20435731
PDF is available here.
PDF is available here.
Journal of Bacteriology (192)13 2010
Abstract
We used a combination of mutagenesis, gene expression, and radiotracer uptake analyses to functionally characterize the role of these PBPs and associated gene clusters. Quantitative PCR (qPCR) demonstrated that pstS1 was expressed at a high level in P(i)-replete conditions compared to sphX or pstS2....
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PMID: 20435726
PDF is available here.
PDF is available here.
Journal of Bacteriology (192)13 2010
Abstract
We show that mutations in the RcsCDB phosphorelay system restored virulence and motility in a D. dadantii opg-negative strain, indicating a relationship between the Rcs phosphorelay and OPGs....
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PMID: 20418397
PDF is available here.
PDF is available here.
Abstract
We describe technologies for detecting these young retrotransposon insertions and demonstrate that such insertions indeed are abundant in human populations. We also found that new somatic L1 insertions occur at high frequencies in human lung cancer genomes. Genome-wide analysis suggests that altered...
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PMID: 20603005
PDF is available here.
PDF is available here.
Journal of Molecular Biology (399)4 2010
Abstract
We study the elastic behavior of in vitro networks of the intermediate filament protein vimentin. Rheological experiments reveal that vimentin networks stiffen with increasing concentrations of Ca(2+) and Mg(2+), showing that divalent cations act as crosslinkers. We quantitatively describe the elast...
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PMID: 20447406
PDF is available here.
PDF is available here.
Abstract
We present the results of combining design and selection to remodel a protein-peptide binding interface, using the peptide PTIEEVD and the TPR1 module interaction as our test case. We initially used the program Rosetta to interrogate possible TPR1 sequences compatible with binding the peptide PTIEEV...
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PMID: 20304973
PDF is available here.
PDF is available here.
Abstract
We used transposon-mediated mutagenesis to knock out genes in rat spermatogonial stem cells. Given the capacity of the testis to support spermatogenesis from thousands of transplanted, genetically manipulated spermatogonia, this approach paves a way for high-throughput functional genomic studies in...
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PMID: 20473302
PDF is available here.
PDF is available here.
Abstract
The nmf240 phenotype closely resembles that reported for Clcn2 knockout mice. The observation that heterozygous nmf240 mice present with a reduced ERG light peak component suggests that CLCN2 is necessary for the generation of this response component....
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PMID: 20071672
PDF is available here.
PDF is available here.
Cell Calcium (47)6 2010
Abstract
We evaluate recent studies which have used a similar approach to investigate key residues underlying the in vivo modulation by select regulatory factors. In addition, we review studies defining the structural requirements in the channel domain which comprise the conduction pathway and are suggested...
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PMID: 20510450
PDF is available here.
PDF is available here.
Microbiology (156)Pt 6 2010
Abstract
We identify hmuP in a genetic screen for mutants that displayed aberrant control of shmR. The normal induction of shmR in response to iron limitation was lost in the hmuP mutant, showing that this gene positively affects shmR expression. Moreover, the HmuP protein is not part of the haemin transport...
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PMID: 20167620
PDF is available here.
PDF is available here.
Microbiology (156)Pt 6 2010
Abstract
We identify positions within the histidine kinases and response regulators of the WalRK and PhoPR two-component systems of Bacillus subtilis that make a major contribution to the specificity of phosphotransfer. Changing the identity of the amino acid at position 11 within the alpha1 helix of WalK an...
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PMID: 20167622
PDF is available here.
PDF is available here.
Abstract
Rhodococcus rhodochrous PA-34 has been reported to produce nitrile hydratase enzyme that converts 3-cyanopyridine to nicotinamide. A mutant of R. rhodochrous PA-34 was generated through chemical mutagenesis using N-methyl-N-nitro-N-nitrosoguanidine (MNNG) that exhibited 2 times higher nitrile hydrat...
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PMID: 20587386
PDF is available here.
PDF is available here.
Abstract
We find that circularization of the HIV-1 genome does not affect the initiation of reverse transcription, but stimulates the first strand transfer during reverse transcription in vitro, underscoring the functional importance of the interaction....
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PMID: 20430859
PDF is available here.
PDF is available here.
Abstract
These results were consistent with the in vivo finding that there was little difference in the mutagenic efficiencies of (+)-BDO2 versus meso-BDO2 in rodents. Thus, in terms of mutagenic potency, there was no evidence that stereochemical configurations of BDO and BDO2 play a significant role in the...
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PMID: 20853577
PDF is available here.
PDF is available here.
Abstract
We designed a screening procedure to enrich for phenotypes with defects in induced egress. The procedure is based on in vitro induced egress and the efficient separation of intracellular from extracellular parasites. Attachment and fast reinvasion of egressed parasites are prevented by the addition...
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PMID: 20227445
PDF is available here.
PDF is available here.
Abstract
Fluorinated derivatives of 1,4-naphthoquinone are highly potent inhibitors of Cdc25A and Cdc25 phosphatases and growth of tumor cells. Five new N-substituted polyfluorinated derivatives of 2-amino-1,4-naphthoquinone were synthesized and their mutagenic and antioxidant properties in Salmonella cells,...
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PMID: 20189692
PDF is available here.
PDF is available here.
Abstract
We begun to comprehend the depth, breadth, and complexity of these pathways and of their interrelationships. Here, we summarize successful examples in which different forward genetic approaches have led to novel discoveries in NF-kappaB signaling. We believe that forward genetics will continue to pl...
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PMID: 20516132
PDF is available here.
PDF is available here.
Journal of Neuroscience (30)22 2010
Abstract
We identify neurexin-1beta lacking an insert at splice site 4 (-S4) as a ligand for LRRTM2. Neurexins bind LRRTM2 with a similar affinity but distinct code from the code for binding neuroligin-1 (the predominant form of neuroligin-1 at glutamate synapses, containing the B splice site insert). Wherea...
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PMID: 20519524
PDF is available here.
PDF is available here.
Molecular Pharmacology (77)6 2010
Abstract
We report that aspirin maximally acetylates one monomer of human (hu) PGHS-2. The acetylated monomer of aspirin-treated huPGHS-2 forms 15-hydroperoxyeicosatetraenoic acid from AA, whereas the nonacetylated partner monomer forms mainly PGH(2) but only at 15 to 20% of the rate of native huPGHS-2. Thes...
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PMID: 20194532
PDF is available here.
PDF is available here.
Abstract
RNA virus polymerases must initiate replicative RNA synthesis with extremely high accuracy to maintain their genome termini and to avoid generating defective genomes. For the single-stranded negative-sense RNA viruses, it is not known how this accuracy is achieved. To investigate this question, muta...
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PMID: 20479224
PDF is available here.
PDF is available here.
Journal of Antibiotics (63)6 2010
Abstract
We performed the chemical mutagenesis of Halobacillus halophilus (the producer of a C(30) carotenoid, methyl glucosyl-3,4-dehydro-apo-8'-lycopenoate) to isolate novel carotenoids that are biosynthetic intermediates of methyl glucosyl-3,4-dehydro-apo-8'-lycopenoate. As a result, we isolated two novel...
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PMID: 20414323
PDF is available here.
PDF is available here.
Abstract
We have identified a single cysteinyl residue in the TAP complex that modulates peptide binding and translocation, thereby restricting the epitope repertoire. Cysteine 213 in human TAP2 was found to be part of a newly uncovered substrate-binding site crucial for peptide recognition. This residue con...
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PMID: 20439763
PDF is available here.
PDF is available here.