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Genes, Insect (0):
Recent article
Genes, Insect
Abstract
We provide an overview of Drosophila lens development, discussing intrinsic and extrinsic factors controlling lens cell specification and differentiation. We then summarize key morphological and molecular events in vertebrate lens development, emphasizing regulatory factors and networks strongly ass...
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PMID: 21877135
PDF is available here.
PDF is available here.
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Abstract
We report the identification and validation of suitable reference genes in the bed bug, Cimex lectularius, by using qRT-PCR. The expression stability of eight reference genes in different tissues (abdominal cuticle, midgut, Malpighian tubules, and ovary) and developmental stages (early instar nymphs...
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PMID: 21845960
PDF is available here.
PDF is available here.
Abstract
We show that a clock in the Drosophila fat body drives rhythmic expression of genes involved in metabolism, detoxification, the immune response, and steroid hormone regulation. Some of these genes cycle even when the fat body clock is disrupted, indicating that they are regulated by exogenous factor...
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PMID: 21641546
PDF is available here.
PDF is available here.
Abstract
We used wheat lacking H genes to compare the survival and growth of a nonadapted strain to two H-adapted strains. The two adapted strains differed in that one had been selected for adaptation to H9, whereas the other strain had been selected for adaptation to H13. Tests showed that two H-adapted str...
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PMID: 21735929
PDF is available here.
PDF is available here.
Abstract
Helicoverpa armigera, a polyphagous lepidopteron insect pest causes severe yield loss in cotton, legumes, tomato, okra and other crops. Application of chemical pesticides although effective, has human health and environmental safety concerns. Moreover, development of resistance against most of the av...
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PMID: 21702227
PDF is available here.
PDF is available here.
Abstract
Abstract
We report mutants of a novel Drosophila gene twenty-four (tyf) that show weak behavioural rhythms. Weak rhythms are accompanied by marked reductions in the levels of the clock protein Period (PER) as well as more modest effects on Timeless (TIM). Nonetheless, PER induction in pacemaker neurons can r...
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PMID: 21331043
PDF is available here.
PDF is available here.
Abstract
We have analysed the overlap of candidate genes identified from studies of gene expression in Drosophila melanogaster using similar technical platforms. We found little overlap across studies between putative candidate genes for the same traits in the same sex. Instead there was a high degree of ove...
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PMID: 21283582
PDF is available here.
PDF is available here.
Abstract
We address this question using species-specific microarrays designed from six sequenced Drosophila species separated by up to 40 million years. We quantify divergence at different times during embryogenesis, and show that expression is maximally conserved during the arthropod phylotypic period. By f...
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PMID: 21150996
PDF is available here.
PDF is available here.
Developmental Biology (347)1 2010
Abstract
We genetically created such neuroblasts, and found that in embryos, they were initially mitotic and then gradually differentiated into neurons, frequently forming a clone of cells homogeneous in temporal identity. By contrast, larval neuroblasts with the same genotype continued to proliferate withou...
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PMID: 20599889
PDF is available here.
PDF is available here.
Abstract
We demonstrate a role for Lon in mtDNA metabolism. An RNA interference (RNAi) construct was designed that reduces Lon to less than 10% of its normal level in Drosophila Schneider cells. RNAi knockdown of Lon results in increased abundance of mitochondrial transcription factor A (TFAM) and mtDNA copy...
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PMID: 20930118
PDF is available here.
PDF is available here.
Abstract
In this paper, the complete mitochondrial genome of Acraea issoria (Lepidoptera: Nymphalidae: Heliconiinae: Acraeini) is reported; a circular molecule of 15,245 bp in size. For A. issoria, genes are arranged in the same order and orientation as the complete sequenced mitochondrial genomes of the oth...
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PMID: 20091125
PDF is available here.
PDF is available here.
Abstract
We present the complete mitochondrial DNA sequence of Eupolyphaga sinensis. This closed circular molecule is 15553 bp long and consists of 37 genes that encode for 13 inner membrane proteins, 2 ribosomal RNAs and 22 transfer RNAs. The genome shares the gene order and orientation with previously know...
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PMID: 20012368
PDF is available here.
PDF is available here.
Abstract
A Bacterial Artificial Chromosome (BAC) library was made from wild-caught Simulium squamosum, which is an important vector of human onchocerciasis. The library is composed of 12,288 BACs, with an average insert size of 128 kb, and is expected to contain ~1.54 GB of cloned DNA. Random BAC-end sequenc...
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PMID: 20603211
PDF is available here.
PDF is available here.
Abstract
The Old World screwworm fly (OWS), Chrysomya bezziana Villeneuve (Diptera: Calliphoridae), is a myiasis-causing blowfly of major concern for both animals and humans. Surveillance traps are used in several countries for early detection of incursions and to monitor control strategies. Examination of s...
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PMID: 20497318
PDF is available here.
PDF is available here.
Abstract
The mitochondrial DNA of the cattle grub Hypoderma lineatum (de Villers) (Diptera: Oestridae) was completely sequenced. The entire molecule was 16,354 bp long and presented a heavy bias towards A + T, which accounted for 77.8% of the whole genome. Hypoderma lineatum genes were organized in the same...
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PMID: 20497317
PDF is available here.
PDF is available here.
Genetika (Moskva) (46)9 2010
Abstract
We observed unusually high mobility of flies and high sexual activity of males carrying these mutations. We used special tests for evaluation of energy metabolism of CDL mutants. Indirect calorimetry (CO2 excretion measurement) has been used for estimation of energy exchange in four mutant and two c...
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PMID: 21061617
PDF is available here.
PDF is available here.
Genetika (Moskva) (46)9 2010
Abstract
We investigated role of LTR retrotransposons in expression of genes in Drosophila. Using database of spliced ESTs we identified only 13 potential cases of transcription initiation from long terminal repeats of LTR within euchromatic part of the Drosophila genome. Our results indicate on insignifican...
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PMID: 21061618
PDF is available here.
PDF is available here.
Abstract
We found a novel piggyBac-like DNA transposon that potentially encodes an intact transposase (610 amino acid residues), which is flanked by 16-bp perfect inverted terminal repeats and a duplicated TTAA target site. Interestingly, we also identified another intact copy of this transposon on an autoso...
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PMID: 20725145
PDF is available here.
PDF is available here.
Genetics Research (92)4 2010
Abstract
TP5, a P element inserted in the telomere-associated sequences of the X chromosome, represses the excision of other P elements in the germ line through a combination of maternal and zygotic effects. The maternal component of this repression is impaired by heterozygous mutations in the aubergine and...
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PMID: 20943007
PDF is available here.
PDF is available here.
Abstract
We show that Smo activation is dependent on the levels of the phospholipid phosphatidylinositol-4 phosphate (PI4P). Loss of STT4 kinase, which is required for the generation of PI4P, exhibits hh loss-of-function phenotypes, whereas loss of Sac1 phosphatase, which is required for the degradation of P...
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PMID: 20643350
PDF is available here.
PDF is available here.
Developmental Cell (19)1 2010
Abstract
We show that conventional models of Delta-Notch signaling cannot account for bristle spacing or the gradual refinement of this pattern. Instead, the pattern refinement we observe using live imaging is dependent upon dynamic, basal actin-based filopodia and can be quantitatively reproduced by simulat...
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PMID: 20643352
PDF is available here.
PDF is available here.
Abstract
Abstract
We show that peptides of 11 to 32 amino acids encoded by the polished rice (pri) sORF gene control epidermal differentiation in Drosophila by modifying the transcription factor Shavenbaby (Svb). Pri peptides trigger the amino-terminal truncation of the Svb protein, which converts Svb from a represso...
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PMID: 20647469
PDF is available here.
PDF is available here.
Developmental Biology (343)1-2 2010
Abstract
We have determined the preferred DNA binding sequence of Opa by SELEX and shown that it is necessary and sufficient to activate transcription of reporter gene constructs under Opa control in transgenic flies. We have found a related sequence in the enhancer region of an opa-responsive gene, sloppy p...
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PMID: 20403347
PDF is available here.
PDF is available here.
Abstract
We examined the motif 10 element (MTE), which is located downstream of the transcription start site and acts in conjunction with the initiator (Inr). We found that the MTE promotes the binding of purified TFIID to the core promoter and that the TAF6 and TAF9 subunits of TFIID appear to be in close p...
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PMID: 20457814
PDF is available here.
PDF is available here.
Abstract
We implicate Drifter/Ventral veinless (Dfr/Vvl), a class III POU domain transcription factor, in tissue-specific regulation of the innate immune defense of Drosophila. We show that Dfr/Vvl is highly expressed in a range of immunocompetent tissues, including the male ejaculatory duct, where its prese...
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PMID: 20457811
PDF is available here.
PDF is available here.
Zhongguo ji sheng chong xue yu ji sheng chong bing za zhi = Chinese journal of parasitology & parasitic diseases (28)3 2010
Abstract
To express the genes of adenosine deaminase (ADA), C-lectin and serpin (serine protease inhibitor) in the salivary gland of Aedes albopictus.
Total RNA was extracted respectively from salivary glands of unfed (group SG) and engorged adult female Ae. albopictus mosquitoes (group BSG), female carcasse...
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PMID: 20806502
PDF is available here.
PDF is available here.
Abstract
We describe the chimeric gene Quetzalcoatl (Qtzl; CG31864), which formed in the recent past and swept to fixation in D. melanogaster. Qtzl arose through a duplication on chromosome 2L that united a portion of the mitochondrially targeted peptide CG12264 with a segment of the polycomb gene escl. The...
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PMID: 20534482
PDF is available here.
PDF is available here.
Abstract
We used a group of closely related species, the small ermine moths (Lepidoptera: Yponomeutidae) as a model to investigate the role of FARs in the biosynthesis of complex pheromone blends. Homology-based molecular cloning in three Yponomeuta species led to the identification of multiple putative FAR...
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PMID: 20534481
PDF is available here.
PDF is available here.
Abstract
We appealed to alternative nuclear sequences of 27 ribosomal proteins (RPs) to generate a data matrix of 10,731 nucleotides for 22 holometabolan taxa, including two strepsipteran species. Phylogenetic relationships among holometabolan insects were analyzed under several nucleotide-coding schemes to...
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PMID: 20348001
PDF is available here.
PDF is available here.
Nucleic Acids Research (38)11 2010
Abstract
We present model-based gene set analysis (MGSA) that analyzes all categories at once by embedding them in a Bayesian network, in which gene response is modeled as a function of the activation of biological categories. Probabilistic inference is used to identify the active categories. The Bayesian mo...
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PMID: 20172960
PDF is available here.
PDF is available here.
Abstract
We have cloned the cDNA of the first receptor known to be involved in an antidiuretic strategy in insects, a strategy that prevents diuresis. This receptor belongs to the insect CAPA receptor family known in other insects to be activated by peptides encoded within the capability gene. We characteriz...
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PMID: 20479227
PDF is available here.
PDF is available here.
Abstract
We report the occurrence of two genes homologous to the eukaryotic Elongation Factor 1A in Bradysia hygida and describe the partial cloning and characterization of the B. hygida eukaryotic Elongation Factor 1A-F1 (BheEF1A-F1) gene. The pattern of BheEF1A-F1 expression in the salivary gland at the en...
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PMID: 20414584
PDF is available here.
PDF is available here.
Abstract
A molecular phylogeny of the subtribe Calathina was inferred from DNA sequence data from the mitochondrial cox1-cox2 region and the nuclear genes 28S and EF-1alpha. All lineages within Calathina from the Holarctic region were represented except for the monotypic subgenus Tachalus. Maximum Parsimony...
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PMID: 19900569
PDF is available here.
PDF is available here.
Journal of Neuroscience (30)18 2010
Abstract
We inactivated DSiaT in vivo by gene targeting and analyzed phenotypes of DSiaT mutants using a combination of behavioral, immunolabeling, electrophysiological, and pharmacological approaches. Our experiments demonstrated that DSiaT expression is restricted to a subset of CNS neurons throughout deve...
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PMID: 20445073
PDF is available here.
PDF is available here.
Abstract
We showed that one copy of the genes diverged rapidly and in one case (ago1b) shows signs of positive selection. These expansions occurred concomitantly within a brief evolutionary period. The pea aphid, which reproduces by viviparous parthenogenesis, is able to produce several adapted phenotypes fr...
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PMID: 20179251
PDF is available here.
PDF is available here.
Development (137)10 2010
Abstract
We used loss- and gain-of-function experiments in the hover fly Episyrphus balteatus (Syrphidae) to address the question of how the maternal regulation of gap genes evolved. We find that, in Episyrphus, a highly diverged bicoid ortholog is solely responsible for the AP polarity of the embryo. Episyr...
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PMID: 20430746
PDF is available here.
PDF is available here.
Abstract
We review the different forms of meiotic drive and their possible roles in speciation. We discuss the recent empirical evidence for a link between drive and hybrid male sterility, also suggesting a possible mechanistic explanation for this link in the context of chromatin remodelling. Finally, we re...
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PMID: 20308102
PDF is available here.
PDF is available here.
Developmental Biology (340)2 2010
Abstract
We identify a novel allele of bazooka (baz), the Drosophila homolog of Par-3, which has allowed the analysis of baz function throughout oogenesis. We demonstrate that baz is required for spatial restriction of the germ plasm and axis patterning, and we uncover multiple requirements for baz in regula...
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PMID: 20152826
PDF is available here.
PDF is available here.
Current Biology (20)7 2010
Abstract
Taking these results together, we demonstrate that the DN1(p)s lie at the nexus of PDF and photic signaling to produce appropriate daily behavior....
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PMID: 20362452
PDF is available here.
PDF is available here.
Current Biology (20)7 2010
Abstract
We show that the absence of adult neurogenesis in Drosophila results from the elimination of neural stem cells (neuroblasts) during development. Prior to their elimination, their growth and proliferation slows because of decreased insulin/PI3 kinase signaling, resulting in nuclear localization of Fo...
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PMID: 20346676
PDF is available here.
PDF is available here.
Current Biology (20)7 2010
Abstract
We report on two mutants, ocelliless(1) and tay bridge(1), that, although making a correct decision to climb, fail dramatically in aiming at the right direction. Both mutants show structural defects in the protocerebral bridge, a central complex neuropil formed like a handlebar spanning the brain he...
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PMID: 20346674
PDF is available here.
PDF is available here.
Current Biology (20)7 2010
Abstract
These studies identify Crb as a novel SWH regulator via JM-dependent effects on Ex levels and localization and suggest that discrete domains within Crb may allow it to integrate junctional polarity signals with a conserved growth pathway....
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PMID: 20362445
PDF is available here.
PDF is available here.
Current Biology (20)7 2010
Abstract
We present a structure-function analysis of Baz focusing on its subcellular localization and function in four different polarized Drosophila cell types: the embryonic ectodermal epidermis, the follicular epithelium, embryonic neuroblasts, and the oocyte. We show that the PDZ domains of Baz are dispe...
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PMID: 20303268
PDF is available here.
PDF is available here.
Current Biology (20)7 2010
Abstract
Collectively, our data reveal that the cell polarity regulators Lgl, aPKC, and Crumbs regulate the SWH pathway by two distinct pathways: Lgl acts antagonistically to aPKC to regulate Hippo and RASSF localization, whereas Crumbs regulates Expanded localization. Thus, our study implicates Lgl, aPKC, a...
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PMID: 20362447
PDF is available here.
PDF is available here.
Current Biology (20)7 2010
Abstract
Fruit flies with genetic lesions disrupting the structure of a brain region known as the protocerebral bridge fail to aim their movements correctly when crossing gaps, implicating this central brain neuropile in the visual control of goal-directed behaviour.
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PMID: 20392418
PDF is available here.
PDF is available here.
Current Biology (20)7 2010
Abstract
We turned to Drosophila, where a single gene, CG11063 or djub, exists. Drosophila lacking the djub gene or depleted of dJub by RNA interference identify djub as an essential gene for development and a novel regulator of epithelial organ size as a component of the conserved Hippo (Hpo) pathway, which...
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PMID: 20303269
PDF is available here.
PDF is available here.
Genes & Development (19)2 2010
Abstract
Milk proteins are an essential component of viviparous reproduction in the tsetse fly. Milk proteins are synthesized in and secreted from the milk gland tissue and constitute 50% of the secretions from which the intrauterine larva derives its nourishment. To understand milk protein function and regul...
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PMID: 20136662
PDF is available here.
PDF is available here.
Genetics Research (92)2 2010
Abstract
We studied the regeneration by spontaneous mutation of the genetic variance for two metric traits [abdominal (AB) and sternopleural (ST) bristle number] and that of the concealed mutation load for viability, together with their temporal stability, using alternative selection models based on mutation...
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PMID: 20515513
PDF is available here.
PDF is available here.
Genetics Research (92)2 2010
Abstract
We investigate the role of molecular variation in senescence marker protein-30 (Smp-30) on natural phenotypic variation in cold tolerance in Drosophila melanogaster. Smp-30 encodes a product that is thought to be involved in the regulation of Ca2+ ion homeostasis and has been shown previously to be...
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PMID: 20515514
PDF is available here.
PDF is available here.
Abstract
Triatoma (T.) dimidiata is a hematophagous Hemiptera and a main vector of Chagas disease. The saliva of this and other blood-sucking insects contains potent pharmacologically active components that assist them in counteracting the host hemostatic and inflammatory systems during blood feeding. To des...
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PMID: 19900580
PDF is available here.
PDF is available here.
Abstract
We sequenced over 3300bp of nucleotide sequence data from the 28S ribosomal DNA and CAD (rudimentary) genes from 29 taxa representing all opomyzoid families, as well as 13 outgroup taxa. Relationships recovered differed between analyses, and only branches supporting well-established monophyletic fam...
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PMID: 20040375
PDF is available here.
PDF is available here.
Abstract
We have investigated an insect-bacteria relationship with a non-nutritional basis. The reed beetles (Donaciinae) harbor bacteria that produce a secretion used by the larvae for building a cocoon for pupation in mud underwater. The 16S rRNA of the bacteria and the cytochrome c oxidase I and elongatio...
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PMID: 19900566
PDF is available here.
PDF is available here.
Abstract
We analyzed the expression pattern of 10 melanin-related genes in P. xuthus, and analyzed whether these genes were responsible for Bombyx larval color mutants. We found that seven genes correlated strongly with the stage-specific larval cuticular markings of P. xuthus, suggesting that, compared with...
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PMID: 20433456
PDF is available here.
PDF is available here.
Abstract
Paralogous genes frequently show differences in patterns and rates of substitution that are typically attributed to different selection regimes, mutation rates, or local recombination rates. Here, two anciently diverged paralogous copies of the histone H3 gene in sea stars, the tandem-repetitive ear...
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PMID: 20433461
PDF is available here.
PDF is available here.
Abstract
We have newly identified three bacteria-induced genes from the silkworm Bombyx mori by quantitative reverse transcriptase-polymerase chain reaction. One of these, eukaryotic initiation factor 4E-1 (eIF4E-1), is assumed to encode an eIF4E family, which plays a role in the initiation of translation as...
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PMID: 20077574
PDF is available here.
PDF is available here.
Abstract
We studied the gene family encoding CTLPs in the genome of the red flour beetle, Tribolium castaneum. Using an extended search pattern, we identified 14 TcCTLP genes that encode peptidases with S1 specificity pocket residues typically found in chymotrypsin-like enzymes. We further analyzed the expre...
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PMID: 19897036
PDF is available here.
PDF is available here.
Abstract
We identify Kibra as another upstream component of the Hippo signaling pathway. We show that Kibra functions together with Mer and Ex in a protein complex localized to the apical domain of epithelial cells, and that this protein complex regulates the Hippo kinase cascade via direct binding to Hpo an...
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PMID: 20159598
PDF is available here.
PDF is available here.
Abstract
Four housekeeping genes named Lbbeta-Actin1, Lbbeta-Actin2, Lbalpha-Tubulin, and LbGapdh were cloned from Liposcelis bostrychophila using the combined techniques of reverse transcriptase-PCR (RT-PCR) with rapid amplification of cDNA ends (RACE). The GenBank accession numbers were FJ196622, FJ447483,...
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PMID: 19757170
PDF is available here.
PDF is available here.