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Bacteriocin Plasmids (0):
Recent article
Bacteriocin Plasmids
Abstract
We review the current knowledge on the molecular stress adaptation responses in L. monocytogenes cells as revealed through transcriptome, proteome, genetic, and physiological analysis. The adaptation of L. monocytogenes to stress exposure is achieved through global expression changes in a large numb...
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PMID: 21495855
PDF is available here.
PDF is available here.
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Journal of Antibiotics (63)7 2010
Abstract
Deoxyactagardine B (DAB) is a hitherto unknown type B lantibiotic, produced by Actinoplanes liguriae NCIMB41362. The mature peptide is 19 amino acids in length and structurally analogous to actagardine, differing by two amino acids (V15L and I16V) and the absence of a sulfoxide bond between residues...
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PMID: 20520597
PDF is available here.
PDF is available here.
Abstract
Four strains (M060106-1, M061122-6, M061122-10 and M061122-12) were tagged with GFP, showing high plasmid stability. These tagged strains possessed the basic characteristics identical to their original strains and, hence, were fit for short-term study of environmental colonization. All four GFP-tagg...
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PMID: 19637569
PDF is available here.
PDF is available here.
Abstract
These results demonstrated the diverse patterns of antibacterial substance production and the possibility of the existence of new antibacterial substance(s) produced by P. carotovorum subsp. carotovorum isolated in Korea....
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PMID: 19190407
PDF is available here.
PDF is available here.
Abstract
We demonstrate that three alleles of iss occur among E. coli isolates that appear to have evolved from a common lambda bor precursor. In addition to the occurrence of iss on the ColV/BM virulence plasmids, at least two iss alleles occur within the E. coli chromosome. One of these alleles (designated...
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PMID: 18281426
PDF is available here.
PDF is available here.
Abstract
Representatives of Bifidobacterium genus are considered to play many important roles in intestinal homeostasis. On the other hand, their molecular biology and genetics have been poorly studied. In order to broaden our understanding of their health-promoting mechanisms, it is extremely important to p...
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PMID: 18368765
PDF is available here.
PDF is available here.
BioTechniques (43)6 2007
Abstract
We have developed a novel vector pTCS, as a tool for efficient selection of open reading frame (ORF)-containing inserts. In pTCS clones containing an insert with an ORF a downstream marker gene (immE3, conferring resistance to colicin) is activated via translational coupling with the insert, and tra...
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PMID: 18251250
PDF is available here.
PDF is available here.
Abstract
We showed that the region rich in A and U between the stem-loop II structure and GA cluster sequence, formation of the stem-loop II structure, but not its sequence, and the region between the GA cluster sequence and initiation codon are important along with the GA cluster sequence for efficient tran...
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PMID: 17720244
PDF is available here.
PDF is available here.
Abstract
In an effort to exploit this system for the heterologous expression of enterocin AS-48, a number of vectors containing the as-48 cluster were constructed and used to transform several LAB strains (genera Enterococcus, Lactococcus and Lactobacillus) CONCLUSION: Heterologous production of enterocin AS...
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PMID: 17448170
PDF is available here.
PDF is available here.
Analytical Biochemistry (363)1 2007
Abstract
Abstract
I and J (also named as enterocins L50A and L50B). After comparison to sequences in public databases, analysis of the gene organization of pEF1 suggests a modular structure with three different functional domains: the replication region, the bacteriocin region and the mobilization plus UV-resistance...
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PMID: 16893567
PDF is available here.
PDF is available here.
Journal of Bacteriology (189)6 2007
Abstract
We demonstrated that region B and site beta play a critical role for stable Rep protein-origin binding and, furthermore, that 284-Thr in this region of the ColE2 Rep protein and the corresponding 293-Trp of the ColE3 Rep protein mainly determine the Rep-origin binding specificity. On the other hand,...
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PMID: 17237180
PDF is available here.
PDF is available here.
Microbiology (152)Pt 12 2006
Abstract
I were analysed in vivo and in vitro. In an amino-acid-starved relA mutant, in which uncharged tRNAs occur in large amounts, three products of specific cleavage of RNA I were observed, in contrast to an otherwise isogenic relA(+) host. Overexpression of tRNA(Ala)(UGC), which under such conditions oc...
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PMID: 17159198
PDF is available here.
PDF is available here.
Abstract
This study investigated the occurrence of virulence-associated genes, including stx1, stx2, stx2c, stx2d, stx2e, eae and its subtypes (alpha, beta, gamma, epsilon), efa1, cdt-V cluster, enterohaemorrhagic Escherichia coli (EHEC)-hlyA, katP, espP, etpD, sfpA and the flagellar fliC gene, in nine sorbi...
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PMID: 16774559
PDF is available here.
PDF is available here.
Journal of Bacteriology (188)3 2006
Abstract
We analyzed the structural and functional organization of the ColE2 origin by using various derivatives carrying deletions and single-base-pair substitutions. The origin may be divided into three subregions: subregion I, which is important for stable binding of the Rep protein; subregion II, which i...
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PMID: 16428404
PDF is available here.
PDF is available here.
Microbiology (152)Pt 1 2006
Abstract
The complete nucleotide sequence of the 3475 bp plasmid pCD3.4 from Carnobacterium divergens LV13, which encodes the bacteriocin divergicin A, was determined. Nucleotide sequence, deletion and complementation analyses revealed the presence of a trans-acting replication protein, RepA, and DNA sequenc...
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PMID: 16385127
PDF is available here.
PDF is available here.
Abstract
Ability to secrete bacteriocines and microcines was studied in 25 cultures of lactobacilli isolated from intestine of healthy children. Sixteen (64%) of them produced microcines with wide spectrum of antagonistic activity. Susceptibility of microcines-secreting cultures to antibacterial preparations...
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PMID: 17297886
PDF is available here.
PDF is available here.
FEMS Microbiology Letters (78)2-3 2006
Abstract
Escherichia coli strains harbouring pBR332 and pBR329 plasmids, when treated with Cis-DDP, a novel curing agent, resulted in curing of the plasmids with 100% frequency.
PDF is available here.
PDF is available here.
Zhonghua Yixue Zazhi (85)43 2005
Abstract
Five vectors expressing the siRNAs targeting the HBV P region, pGE-HBVP1-pGE-HBV5 were successfully constructed. The efficiency of transfection of the vectors into the 2.2.15 cells were 30% to 40%. 24, 48, 72, and 96 hours after the transfection of pGE-HBVP2, the strongest inhibitor among the five,...
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PMID: 16324412
PDF is available here.
PDF is available here.
Abstract
Cryptic plasmid pRK2 of the strain Escherichia coli W (ATCC 9637), an ancestor of production strains for penicillin G acylase, was sequenced and characterized. Based on the data on replication region and origin (ori sequence AAC, 924-926nt), the plasmid was classified as ColE1-like plasmid. DNA sequ...
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PMID: 15907542
PDF is available here.
PDF is available here.
Abstract
We have shown that bacteriocins Ent1071A and Ent1071B act independently against target bacteria....
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PMID: 15718027
PDF is available here.
PDF is available here.
Abstract
Caries Research (39)5 2005
Abstract
Targeting antigens to antigen-presenting cells by fusion to cytotoxic T lymphocyte-associated antigen 4 (CTLA4) has been shown to be a highly efficient method to enhance the efficacy of DNA vaccines. The purpose of this study was to determine the immunogenicity and protective efficacy of the targete...
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PMID: 16110216
PDF is available here.
PDF is available here.
Abstract
E. faecium strain F58 was isolated because of its broad inhibitory spectrum, including activity against food-borne pathogenic and spoilage bacteria. The antimicrobial substance was produced during the growth phase, with maximum production after 16-20 h of incubation at 30 degrees C, and was stable o...
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PMID: 15960674
PDF is available here.
PDF is available here.
Zhonghua kou qiang yi xue za zhi = Zhonghua kouqiang yixue zazhi = Chinese journal of stomatology (39)6 2004
Abstract
pGJA-P could be correctly expressed in vivo. pGJA-P generated increased humoral immune response and anticaries efficacy compared with pGLUA-P....
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PMID: 15854314
PDF is available here.
PDF is available here.
Abstract
A cryptic plasmid pC7 was characterized, which was isolated from Lactobacillus paraplantarum C7 derived from Kimchi, a traditional korean vegetable food. The plasmid pC7 is a circular molecule of 2,134 base-pairs in length with a G + C content of 38.5%. The nucleotide sequence analysis revealed the...
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PMID: 15336486
PDF is available here.
PDF is available here.
Abstract
We show that mutA is expressed in both planktonic and biofilm cells, even though mutacin activities are normally detected only in biofilm cells. Furthermore, we confirm that mutR, the gene upstream of the mutacin operon, is required for mutacin I gene expression. The success of this study validates...
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PMID: 15209996
PDF is available here.
PDF is available here.
Molecular Microbiology (53)2 2004
Abstract
The leading region of the conjugal bacterial plasmid ColIb-P9 contains three dispersed repeats of a 328 bp sequence homologous to Frpo, a sequence from plasmid F that acts as a promoter in single-stranded DNA. One of these sequences, ssi3, inactive in the double-stranded form, promoted in vitro tran...
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PMID: 15228523
PDF is available here.
PDF is available here.
Abstract
Several outbreaks of Escherichia coli O157:H7 infections have been associated with contaminated alfalfa seeds. A recently isolated E. coli strain Hu194 was capable of inhibiting 22 strains of E. coli O157:H7 and this inhibition was mediated by the production of a colicin named Hu194. The objectives...
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PMID: 15163583
PDF is available here.
PDF is available here.
Abstract
We found that mitomycin C induced Escherichia coli containing ColE7-K317 can confer limited protection against bacteriophage M13K07 and lambda infection. Our study showed that degree of protection is correlated with the expression level of the ColE7 operon, indicating that colicin E7 alone or the co...
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PMID: 15110756
PDF is available here.
PDF is available here.
Journal of Bacteriology (186)6 2004
Abstract
The complete nucleotide sequences of two bacteriocin-encoding plasmids isolated from Hafnia alvei (pAlvA and pAlvB) were determined. Both plasmids resemble ColE1-type replicons and carry mobilization genes, as well as colicin-like bacteriocin operons. These bacteriocins appear to be chimeras consist...
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PMID: 14996789
PDF is available here.
PDF is available here.
Abstract
We concluded that they belonged to ColE1, ColE2, and rolling-circle-like replicating plasmids. Plasmid pK (4245 bp) is a representative of widely distributed ColE1 plasmids. Plasmid pP (4301 bp) is homologous to ColE2 plasmids and was present predominantly in single-stranded DNA form. The smallest p...
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PMID: 15052312
PDF is available here.
PDF is available here.
Antilisterial activity of lactic acid bacteria isolated from rigouta, a traditional Tunisian cheese.
Abstract
Seven lactococcal strains producing nisin Z were selected and could be useful as bio-preservative starter cultures. Additional experiments are needed to evaluate the promising strain MMT05 as bio-preservative as Enterococci could exert detrimental or beneficial role in foods. SIGNIFICANCE AND IMPACT...
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PMID: 15281944
PDF is available here.
PDF is available here.
Abstract
Promoters and regulatory genes involved in production of sakacin P are suitable for establishing inducible high-level gene expression in L. plantarum. SIGNIFICANCE AND IMPACT OF THE STUDY: This study describes a system for controllable gene expression in lactobacilli, giving some of the highest expr...
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PMID: 15242451
PDF is available here.
PDF is available here.
Abstract
The vaginal flora of a healthy woman is composed of the Doderleïn bacilli (different species of lactobacilli) forming a biofilm on the mucosa. These bacteria have a beneficial effect by inhibiting growth, adhesion or spread of other microorganisms. Different mechanisms are recognized, including sec...
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PMID: 12379833
PDF is available here.
PDF is available here.
Trends in Biotechnology (20)6 2002
Abstract
The demands for recombinant proteins, in addition to plasmid DNA, for therapeutic use are steadily increasing. Bacterial fermentation processes have long been and still are the major tool for production of these molecules. The key objective of process optimization is to attain a high yield of the re...
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PMID: 12007494
PDF is available here.
PDF is available here.
Microbiology (147)Pt 11 2001
Abstract
XerCD-mediated recombination at cer converts multimers of plasmid ColE1 to monomers, maximizing the number of independently segregating molecules and minimizing the frequency of plasmid loss. In addition to XerCD, recombination requires the accessory factors ArgR and PepA. The promoter P(cer), locat...
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PMID: 11700357
PDF is available here.
PDF is available here.
Abstract
I and RNA II, both involved in the regulation of plasmid DNA replication. The replication region also contains the gene encoding a 64-aa Rom-like protein. Inactivation of the putative rom gene by insertion of a kanamycin-resistance cassette resulted in 4.5-fold increase in pEC156-derived plasmid cop...
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PMID: 11591138
PDF is available here.
PDF is available here.
Abstract
Our results here strongly indicate that the affinity of distamycin to DNA, at a low distamycin concentration, depends highly on the DNA sequence, and preferential binding occurs to the sites of four to six successive A-T pairs having two or more successive G-C pairs on both their ends....
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PMID: 11440155
PDF is available here.
PDF is available here.
Abstract
These results suggest that the diversity of phenotypes detected partially explain the multifactorial nature of avian colisepticemia....
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PMID: 11407021
PDF is available here.
PDF is available here.
Abstract
The structure of Ala2Ile2-6, along with molecular modeling results, demonstrate the importance of tight packing of core residues and the adoption of favorable core side chain rotamer values in determining helix-helix interactions in the four-helix bundle fold. Structural disorder at the N and C term...
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PMID: 11188696
PDF is available here.
PDF is available here.
Journal of Molecular Biology (300)1 2000
Abstract
We used a repeated primer extension assay to map in vivo, at the nucleotide level, the 5' end of the nascent strand where initiation and blockage of replication forks occurs. Initiation occurred primarily at the previously defined origin, however, an alternative initiation site was detected 17 bp up...
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PMID: 10864499
PDF is available here.
PDF is available here.
Journal of Molecular Biology (297)1 2000
Abstract
We analyse R1 multistep copy number control and show that it results in the insensitive replication response found experimentally but that it at the same time effectively prohibits the existence of only one plasmid copy in a dividing cell. In combination with the partition system of R1, this can lea...
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PMID: 10704315
PDF is available here.
PDF is available here.
Microbiology (145 ( Pt 10)) 1999
Abstract
The leading region of a plasmid is the first sector to enter the recipient cell in bacterial conjugation. This sector of IncI1 plasmid ColIb-P9 includes genes that are transcribed in a transient pulse early in the conjugatively infected cell to promote establishment of the immigrant plasmid. Evidenc...
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PMID: 10537187
PDF is available here.
PDF is available here.
Microbiology (145 ( Pt 8))8 1999
Abstract
We used DNA hybridization and sequence analysis to find matches to this sequence in the Escherichia coli chromosome. Our failure to find plausible anti-sense targets has led to the suggestion that Rcd may interact directly with a protein target....
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PMID: 10463180
PDF is available here.
PDF is available here.
Acta Crystallographica Section D (55)Pt 7 1999
Abstract
Conventional refinement methods, when applied to even correctly positioned polyalanine models of a target structure, result in a systematic distortion of the molecular geometry and to a concomitant increase in the mean phase difference from the correct phase set. Here, it is shown that iterative rig...
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PMID: 10393296
PDF is available here.
PDF is available here.
Abstract
We cloned the gene into the compatible P15A-derived rom- plasmid pACYC184. In cells carrying both pACYC184 rom+ and pBR322 rom- the presence of the rom gene in trans had little effect on the copy number of pBR322 rom- at fast growth, but it decreased its copy number at slow growth to the same level...
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PMID: 10087214
PDF is available here.
PDF is available here.
Abstract
We have studied a simple eco-evolutionary model of the interaction of bacteria with colicins and show that high diversity of colicins is to be expected. We find two different dynamical modes each with a high diversity: a hyperimmunity mode and a multitoxicity mode. Bacteria are immune to most toxins...
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PMID: 10049618
PDF is available here.
PDF is available here.
Nucleic Acids Research (27)2 1999
Abstract
We propose that transcription transiently dissociates the triplet repeat complementary strands enabling the non-transcribed strand to fold into a hairpin conformation which is then sufficiently stable that replication bypasses the hairpin to give large deletions. If the TRS was not transcribed, fewe...
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PMID: 9862988
PDF is available here.
PDF is available here.
Journal of Biological Chemistry (273)50 1998
Abstract
A series of plasmids were constructed containing two unidirectional ColE1 replication origins in either the same or opposite orientations and their replication mode was investigated using two-dimensional agarose gel electrophoresis. The results obtained showed that, in these plasmids, initiation of...
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PMID: 9837915
PDF is available here.
PDF is available here.
Acta Crystallographica Section D (54)Pt 6 Pt 2 1998
Abstract
The crystal structure of a designed variant of the ColE1 repressor of primer (ROP) protein has been refined with SHELXL93 to a resolution of 1.09 A. The final model with 510 non-H protein atoms, 576 H atoms in calculated positions and 114 water molecules converged to a standard R factor of 10% using...
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PMID: 10089502
PDF is available here.
PDF is available here.
Journal of Biotechnology (64)2-3 1998
Abstract
ColE1-derived plasmids containing different recombinant genes which are controlled by the tac promoter were amplified following induction with IPTG, but no amplification occurred if product formation was not induced. The plasmid copy number of recombinant E. coli increased three- to sixfold within a...
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PMID: 9821676
PDF is available here.
PDF is available here.
Microbiology (144 ( Pt 10))10 1998
Abstract
A 3.1 kb DNA fragment from pBLA8, a Brevibacterium linens cryptic plasmid, containing all the information required for autonomous replication was cloned and sequenced. Using deletion analysis, the fragment essential and sufficient for autonomous replication was delimited to 1.5 kb. This fragment is...
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PMID: 9802024
PDF is available here.
PDF is available here.
Molecular Microbiology (29)5 1998
Abstract
Multicopy plasmids of Escherichia coli are distributed randomly at cell division and, as long as copy number remains high, plasmid-free cells arise only rarely. Copy number variation is minimized by plasmid-encoded control circuits, and the limited data available suggest that deviations are correcte...
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PMID: 9767582
PDF is available here.
PDF is available here.
FEMS Microbiology Letters (164)2 1998
Abstract
Twenty-five fecal Escherichia coli strains of serogroups O6 and O18 from patients with and without intestinal infections were analyzed for fimbrial adhesins pap, prs and sfa, hemolysin, cytonecrotic factor, colicins, capsules, antibiotic resistances, plasmid content and some plasmid encoded characte...
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PMID: 9682472
PDF is available here.
PDF is available here.
Abstract
Journal of Bacteriology (180)11 1998
Abstract
Thin pili of the closely related IncI1 plasmids ColIb-P9 and R64 are required only for liquid mating and belong to the type IV family of pili. They were sedimented by ultracentrifugation from culture medium in which Escherichia coli cells harboring ColIb-P9- or R64-derived plasmids had been grown, a...
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PMID: 9603870
PDF is available here.
PDF is available here.
Journal of Biological Chemistry (273)19 1998
Abstract
We find that the base pairing interaction at the 5'-rGGC-3' sequence in the hexanucleotide motif is important for interaction between Inc RNA and structure I. In addition, the 21-base loop domain of structure I is folded tighter than predicted, with the hexanucleotide sequence at the top. The second...
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PMID: 9565607
PDF is available here.
PDF is available here.
Journal of Biological Chemistry (273)19 1998
Abstract
We show that the RepZ mRNA leader sequence synthesized in vitro indeed contains several stem-loop structures including structures I and III, but not the pseudoknot. However, disruption of structure III, without changing the repZ ribosome-binding site, by means of base substitution and deletion induc...
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PMID: 9565606
PDF is available here.
PDF is available here.
Microbiology (144 ( Pt 2)) 1998
Abstract
Uropathogenic Escherichia coli strains express chromosomal and plasmid-encoded virulence-associated factors such as specific adhesins, toxins and iron-uptake systems. A CoIV plasmid (pRK100) of a uropathogenic strain and its host KS533 were studied. The host strain encodes the K1 capsule, and P and...
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PMID: 9493372
PDF is available here.
PDF is available here.