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Cosmids (0):
Recent article
Cosmids
Abstract
Our experiments demonstrated that FISH was more sensitive than Southern blot at demonstrating low levels of gene amplification and, additionally, permitted assessment of the distribution of cyclin D1 gene among chromosomes....
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PMID: 19967453
PDF is available here.
PDF is available here.
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Molecular Biotechnology (44)3 2010
Abstract
We purified the 55-kDa chitinase (Chi55) from the CB101 culture supernatant by continuous DEAE-Sepharose and Mono-Q anion exchange chromatography. The N-terminal amino acid sequence of the purified chitinase exactly matched the N-terminal sequence of mature Chi1, indicating that the purified chitina...
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PMID: 19960373
PDF is available here.
PDF is available here.
Journal of Infectious Diseases (199)11 2009
Abstract
No significant differences were observed regarding the abilities of the mutant and the wild-type CFT073 strains to colonize the murine urinary tract in single-challenge infection experiments. However, in competitive-colonization experiments, the mutant was significantly outcompeted by the wild-type...
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PMID: 19432551
PDF is available here.
PDF is available here.
Journal of Bacteriology (191)1 2009
Abstract
We show that an ftsK-null mutant produces anucleate spores at the same frequency as the wild-type strain (0.8%). We report that the smc and ftsK deletion-insertion mutants (ftsK' truncation allele) have developmental segregation defects (7% and 15% anucleate spores, respectively). By use of these la...
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PMID: 18978061
PDF is available here.
PDF is available here.
Fungal Genetics and Biology (45)12 2008
Abstract
Expression of several Aspergillus niger genes encoding major secreted, but not vacuolar, protease genes including the major acid protease gene pepA, was shown to be affected in the previously isolated A. niger protease mutant, AB1.13 [Mattern, I.E., van Noort, J.M., van den Berg, P., Archer, D.A., R...
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PMID: 18930158
PDF is available here.
PDF is available here.
Molecular Microbiology (70)5 2008
Abstract
Sinorhizobium meliloti, the endosymbiont of Medicago sativa, can use haem compounds, including haemoglobin and leghaemoglobin, when growing in the free-living state. The components of the system involved in haem acquisition were confirmed to be ShmR, an outer membrane receptor, and HmuTUV, predicted...
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PMID: 18990190
PDF is available here.
PDF is available here.
Abstract
We constructed a heterologous expression system using Aspergillus oryzae. The approximate region covering the citrinin biosynthetic gene cluster from Monascus purpureus was introduced into A. oryzae via an Aspergillus-Escherichia coli shuttle cosmid vector without manipulating the gene structure. Th...
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PMID: 19111642
PDF is available here.
PDF is available here.
Abstract
We attempted to identify genetic markers that may correlate with miltefosine resistance. Using a functional cloning approach, we have isolated a gene from Leishmania infantum that, upon over-expression, confers protection not only against miltefosine, but also against Sb(III), the active principle o...
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PMID: 18457839
PDF is available here.
PDF is available here.
ChemBioChem (9)10 2008
Abstract
We describe a new general technique that can be used to reconstruct large biosynthetic pathways from overlapping cosmids by retrofitting each cosmid with a "recombinogenic cassette" that contains a shared homologous element and orthogonal antibiotic markers. We employed this technique to reconstruct...
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PMID: 18512205
PDF is available here.
PDF is available here.
Abstract
A chromosomal region of Pectobacterium chrysanthemi PY35 that contains of genes for glycogen synthesis was isolated from a cosmid library. The operon consists of glycogen branching enzyme (glgB), glycogen debranching enzyme (glgX), ADP-glucose pyrophosphorylase (glgC), glycogen synthase (glgA), and...
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PMID: 18594899
PDF is available here.
PDF is available here.
Infection and Immunity (76)7 2008
Abstract
We recently identified two loci, mel1 and mel2, that affect macrophage infection by Mycobacterium marinum. The ability of these loci to confer enhanced infection in trans is presumably due to gene dosage effects since their presence on plasmids increases expression from five- to eightfold. Reasoning...
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PMID: 18443095
PDF is available here.
PDF is available here.
Abstract
We report the complete sequence of pANL, which is 46,366 bp in length with 53% GC content and encodes 58 putative ORFs. The pANL plasmid can be divided into four structural and functional regions: the replication origin region, a signal transduction region, a plasmid maintenance region, and a sulfur...
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PMID: 18353436
PDF is available here.
PDF is available here.
Archives of Microbiology (189)5 2008
Abstract
The four overlapping cosmids from the rubradirin producer, Streptomyces achromogenes var rubradiris NRRL 3061, have 58 ORFs within a 105.6 kb fragment. These ORFs harbored essential genes responsible for the formation and attachment of four distinct moieties, along with the genes associated with reg...
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PMID: 18080113
PDF is available here.
PDF is available here.
Abstract
We characterized a t(1;7)(p22;q21) reciprocal translocation in a patient with childhood-onset schizophrenia (COS) and autism using genome mapping and sequencing methods. Based on genomic maps of human chromosome 7 and fluorescence in situ hybridization (FISH) studies, we delimited the region of 7q21...
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PMID: 17879154
PDF is available here.
PDF is available here.
FEMS Microbiology Letters (281)2 2008
Abstract
A metagenomic approach was taken to investigate the genetic basis for the ability of an anaerobic consortium to grow on either 4-chlorobenzoate or 4-bromobenzoate under denitrifying conditions. Degenerate PCR primers were designed for the family of 4-chlorobenzoyl-CoA dehalogenase genes. The primers...
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PMID: 18355280
PDF is available here.
PDF is available here.
Extremophiles (12)2 2008
Abstract
Two clones 9E7 and 21G8 in a metagenomic library of the east Pacific deep-sea sediment were found to contain alkane hydroxylase genes (alkB). The whole insert sequences of the two cosmid clones were determined. The insert sequences of 9E7 and 21G8 are 40 and 35 kb, respectively. Besides alkB, severa...
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PMID: 18087672
PDF is available here.
PDF is available here.
Microbiology (154)Pt 3 2008
Abstract
These results demonstrate that, in contrast to most of the biosynthesis gene clusters from actinomycetes, genes involved in the biosynthesis of elloramycin are located in two chromosomal loci....
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PMID: 18310024
PDF is available here.
PDF is available here.
Abstract
Conjugative plasmids have evolved entry exclusion mechanisms to inhibit redundant DNA transfer from donor cells into recipients harboring isogenic or closely related plasmids. This exclusion phenomenon has been documented in the incompatibility H group (IncH) plasmid R27. A cosmid library representi...
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PMID: 18192011
PDF is available here.
PDF is available here.
Journal of Bacteriology (190)3 2008
Abstract
The initial enzymes and genes involved in the anoxic metabolism of cholesterol were studied in the denitrifying bacterium Sterolibacterium denitrificans Chol-1S(T). The second enzyme of the proposed pathway, cholest-4-en-3-one-Delta1-dehydrogenase (AcmB), was partially purified. Based on amino acid...
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PMID: 18039763
PDF is available here.
PDF is available here.
Abstract
We report that this is the case. The gene encoding the new isoform, called FabV, was isolated by complementation of a conditionally lethal E. coli fabI mutant strain and was shown to restore fatty acid synthesis to the mutant strain both in vivo and in vitro. Like FabI and FabL, FabV is a member of...
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PMID: 18032386
PDF is available here.
PDF is available here.
Abstract
To study CHEK2 duplicon evolution in anthropoids we applied a combination of comparative FISH and in silico analyses. Our comparative FISH results with a CHEK2 fosmid probe revealed the single-copy status of CHEK2 in New World monkeys, Old World monkeys and gibbons. Whereas a single CHEK2 duplicatio...
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PMID: 18831734
PDF is available here.
PDF is available here.
Abstract
We used fluorescence in situ hybridization (FISH) to examine the variation of mtDNA insertions in maize. Twenty overlapping cosmids, representing the 570-kb maize mitochondrial genome, were individually labeled and hybridized to root tip metaphase chromosomes from the B73 inbred line. A minimum of 1...
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PMID: 18202357
PDF is available here.
PDF is available here.
Abstract
We screened clones with mini-Tn5 random insertions for those showing decreased production of siderophore. Among 6,000 clones screened, nine such clones were selected. These clones contain the transposon inserted in VV2_0830 (GenBank accession number) that is a homolog of a nonribosomal peptide synth...
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PMID: 18239413
PDF is available here.
PDF is available here.
Abstract
I describe below the challenges of allelic exchange in M. tuberculosis, the overall mechanism of a suicide vector system, and how it can be used for efficient allelic exchange in this formally intractable organism....
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PMID: 18370077
PDF is available here.
PDF is available here.
Abstract
We developed transgenic mice bearing a human beta-globin locus-containing cosmid. Specifically, we introduced a mutated BP1 binding site (mtBP1) into the promoter of the beta-globin gene sequence of this cosmid construct. In the mtBP1 mice, we detected a more than a 20-fold increase in human beta-gl...
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PMID: 18656399
PDF is available here.
PDF is available here.
Abstract
The Pacific Nodule Province is a unique ocean area containing an abundance of polymetallic nodules. To explore more genetic information and discover potentially industrial useful genes of the microbial community from this particular area, a cosmid library with an average insert of about 35 kb was co...
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PMID: 17850328
PDF is available here.
PDF is available here.
Environmental Science (28)11 2007
Abstract
Applying our optimized direct extraction method, the percentage of large fragment DNA in the total extracted mangrove soil DNA was significant increased. The large fragment metagenome library derived from natural mangrove soil over four seasons was successfully constructed by the optimized DNA extra...
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PMID: 18290491
PDF is available here.
PDF is available here.
Abstract
We report a method for cloning of novel so far unidentified glycosyltransferase genes from different Actinomycetes strain. This was achieved by designing primers after a strategy named consensus-degenerate hybrid oligonucleotide primer (CODEHOP). Using this approach, 22 novel glycosyltransferase enc...
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PMID: 17497146
PDF is available here.
PDF is available here.
Chemistry & Biology (14)6 2007
Abstract
Anthramycin is a benzodiazepine alkaloid with potent antitumor and antibiotic activity produced by the thermophilic actinomycete Streptomyces refuineus sbsp. thermotolerans. In this study, the complete 32.5 kb gene cluster for the biosynthesis of anthramycin was identified by using a genome-scanning...
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PMID: 17584616
PDF is available here.
PDF is available here.
Abstract
A cosmid cloning vector has been constructed that demonstrates high levels of segregational stability in Escherichia coli K12. pPSX is a 14-kilobase vector derived from the IncW plasmid pR388. pPSX is highly stable in E. coli in the absence of antibiotic selection, even while expressing the toxic in...
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PMID: 17218012
PDF is available here.
PDF is available here.
Journal of Bacteriology (189)10 2007
Abstract
We showed that resorcinol degradation by this bacterium is initiated by two oxidative steps, both catalyzed by membrane-associated enzymes that lead to the formation of hydroxyhydroquinone (HHQ; 1,2,4-benzenetriol) and 2-hydroxy-1,4-benzoquinone (HBQ). This study presents evidence for the further de...
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PMID: 17369298
PDF is available here.
PDF is available here.
Abstract
I secretion in Gram-negative bacteria. moxBA has not been observed in other manganese-oxidizing bacteria but homologues were identified in the genomes of several bacteria including Sinorhizobium meliloti 1021 and Agrobacterium tumefaciens C58. These results suggest that moxBA and its homologues cons...
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PMID: 17359266
PDF is available here.
PDF is available here.
Abstract
We recently detected short DNA fragments, which were significantly associated with the extraintestinal virulent phenotype. In this study, we identified four novel genomic DNA regions of the highly virulent uropathogenic E. coli strain JS299 carrying these previously identified DNA fragments. Charact...
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PMID: 17280868
PDF is available here.
PDF is available here.
Abstract
The pRBM4 cosmid, which harbors a putative cluster of genes spanning a 31.8-kb chromosomal region of the ribostamycin producer Streptomyces ribosidificus ATCC 21294, was heterologously expressed in Streptomyces lividans TK24. ESI-MS/MS, HPLC, and LC-ESI MS analyses showed that the transformation gav...
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PMID: 17289380
PDF is available here.
PDF is available here.
Neuroscience (145)3 2007
Abstract
We characterize the functional properties of a segment of the murine methyl cytosine phosphate guanine binding domain-containing factor 3 (MBD3) promoter region. Transient transfection of a chimera consisting of a 1072 base pair region extending upstream from the MBD3 initiation codon fused to a luc...
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PMID: 17289276
PDF is available here.
PDF is available here.
Abstract
These results confirm that the genetic locus cloned from UC 11065 is essential for pyrrolomycin production, and they also confirm that the highly similar locus in A. vitaminophilum encodes pyrrolomycin biosynthetic genes. Sequence analysis revealed that both clusters contain genes encoding the two c...
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PMID: 17158935
PDF is available here.
PDF is available here.
Abstract
Cytotoxin fractions were isolated from Campylobacter jejuni 81116 and semi-purified by size-exclusion liquid chromatography. The fraction showing the strongest toxicity was injected into mice to produce antiserum. The antiserum was used to screen a C. jejuni 81116 cosmid library. Nine genes were ide...
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PMID: 17266719
PDF is available here.
PDF is available here.
Abstract
Culture-independent studies on environmental samples indicate that most bacteria are not readily cultured in the laboratory. The small fraction of bacteria that have been successfully cultured from environmental samples have been a very rewarding source of novel biologically active natural products....
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PMID: 17546026
PDF is available here.
PDF is available here.
ChemBioChem (8)1 2007
Abstract
BMC Biotechnology (7)1 2007
Abstract
We had previously shown that circularization of the 11.2 kb linear DNA of phage P4 inside its viral capsid generates complex knots by the effect of confinement. We demonstrate here that this mechanism is not restricted to the viral genome. We constructed DNA cosmids as small as 5 kb and introduced t...
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PMID: 18154674
PDF is available here.
PDF is available here.
Journal of Heredity (98)5 2007
Abstract
Annotation features from the 1.9-fold whole-genome shotgun (WGS) sequences of domestic cat have been organized into an interactive web application, Genome Annotation Resource Fields (GARFIELD) (http://lgd.abcc.ncifcrf.gov) at the Laboratory of Genomic Diversity and Advanced Biomedical Computing Cent...
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PMID: 17646276
PDF is available here.
PDF is available here.
Journal of Heredity (98)3 2007
Abstract
We report the isolation and identification of microsatellite sequences from multiple genomic libraries for A. aegypti. We identified 6 single-copy simple microsatellites from 3 plasmid libraries enriched for (GA)(n), (AAT)(n), and (TAGA)(n) motifs from A. aegypti. In addition, we identified 5 single...
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PMID: 17420178
PDF is available here.
PDF is available here.
Abstract
Pathogenicity islands and genomic islands (GI) are key elements in the evolution of bacterial virulence and environmental adaptation. In Salmonella enterica, Salmonella pathogenicity islands ISPI) confer important virulence traits; however, many of these loci have not been characterized in molecular...
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PMID: 18363232
PDF is available here.
PDF is available here.
Journal of Biological Chemistry (281)51 2006
Abstract
We report the cloning of a Trypanosoma cruzi gene encoding a solanesyl-diphosphate synthase, TcSPPS. The amino acid sequence (molecular mass approximately 39 kDa) is homologous to polyprenyl-diphosphate synthases from different organisms, showing the seven conserved motifs and the typical hydrophobi...
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PMID: 17062572
PDF is available here.
PDF is available here.
Abstract
We describe the integration of the complete 57-kbp myxothiazol biosynthetic gene cluster reconstituted from two cosmids from a cosmid library of the myxobacterium Stigmatella aurantiaca DW4-3/1 into the chromosome of the thus far best-characterized myxobacterium, Myxococcus xanthus, in one step. The...
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PMID: 16997979
PDF is available here.
PDF is available here.
Journal of Bacteriology (188)24 2006
Abstract
SC7A1 is a cosmid with an insert of chromosomal DNA from Streptomyces coelicolor A3(2). Its insertion into the chromosome of S. coelicolor strains caused a duplication of a segment of ca. 40 kb and delayed actinorhodin antibiotic production and sporulation, implying that SC7A1 carried a gene negativ...
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PMID: 17041057
PDF is available here.
PDF is available here.
International Journal of Cancer (119)10 2006
Abstract
We showed that the inhibition of cell growth by MIM is anchorage independent. We identified and cloned the promoter region of MIM and located the main promoter activity to 276 bp of 5' flanking sequence sited within a CpG island. Analysis of DNA methylation using bisulphite sequencing revealed that...
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PMID: 16921485
PDF is available here.
PDF is available here.
Developmental Biology (299)1 2006
Abstract
We describe fasciculation defects of animals with mutations in the C. elegans gene dig-1 and show that dig-1 encodes a giant molecule (13,100 amino acids) of the immunoglobulin superfamily. Five new alleles of dig-1 were isolated in a screen for mutations affecting the morphology or function of seve...
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PMID: 16928366
PDF is available here.
PDF is available here.
Abstract
Contributions of thymidine kinase (TK) mutations to acyclovir (ACV) resistance were evaluated in herpes simplex virus type 1 recombinant viruses generated using a set of overlapping cosmids and plasmids. Alterations in both conserved and nonconserved regions of the TK gene were shown to confer high...
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PMID: 16982796
PDF is available here.
PDF is available here.
Identification of a novel gene family involved in osmotic stress response in Caenorhabditis elegans.
Abstract
We report the identification of two genes, osm-7 and osm-11, which are related members of a novel gene family. Mutations in either gene lead to high internal levels of glycerol and cause an osmotic resistance phenotype (Osr). These mutants also have an altered defecation rhythm (Dec). Mutations in c...
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PMID: 16980399
PDF is available here.
PDF is available here.
Abstract
We identified a cosmid clone that oxidizes aromatic compounds by using SAO101 genomic DNA. Sequencing analysis revealed that isolated cosmid clone contained ring-hydroxylating dioxygenase genes (narAaAb) with homologies to indene dioxygenase genes of Rhodococcus strain I24 and naphthalene dioxygenas...
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PMID: 16736088
PDF is available here.
PDF is available here.
Genetics Research (88)2 2006
Abstract
We previously demonstrated that the 55E2-E6; 55F3 region on chromosome 2R is involved in the resistance phenomenon. In this paper, we first restricted the Rlb containing region by mapping at the molecular level the breakpoints of the Df(2R)Pc66, Df(2R)P34 and Df(2R)Pc4 deficiencies, using both chrom...
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PMID: 17125583
PDF is available here.
PDF is available here.
Antonie Van Leeuwenhoek (90)1 2006
Abstract
A series of large chromosomal deletions in Streptomyces hygroscopicus 10-22 were aligned on the physical map of the wild-type strain and the mutants were assessed for their ability to produce the aminocyclitol antibiotic 5102-I (jinggangmycin). Twenty-eight mutants were blocked for jinggangmycin pro...
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PMID: 16680521
PDF is available here.
PDF is available here.
Abstract
The validamycin biosynthetic gene cluster was isolated from Streptomyces hygroscopicus var. limoneus KTCC 1715 (IFO 12704) using a pair of degenerated PCR primers designed from the sequence of AcbC, 2-epi-5-epi-valiolone synthase in the acarbose biosynthesis. The nucleotide sequence analysis of the...
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PMID: 16725283
PDF is available here.
PDF is available here.
Journal of Bacteriology (188)14 2006
Abstract
We identified a novel gene (devA) required for proper development. The devA mutant produced only rare aerial hyphae, and those that were produced developed aberrant spore chains that were much shorter than wild-type chains and had misplaced septa. devA encodes a member of the GntR superfamily, a cla...
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PMID: 16816174
PDF is available here.
PDF is available here.
Abstract
The mannopeptimycins are a novel class of lipoglycopeptide antibiotics active against multidrug-resistant pathogens with potential as clinically useful antibacterials. This report is the first to describe the biosynthesis of this novel class of mannosylated lipoglycopeptides. Included here are the c...
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PMID: 16723579
PDF is available here.
PDF is available here.
DNA Sequence (17)3 2006
Abstract
A novel 5-enolpyruvylshikimate-3-phosphate synthase (EPSPS) gene of 1.35 kb was cloned from a cosmid library of Halomonas variabilis HTG7, inserted into vector pET-28a (+) and transformed in Escherichia coli BL21 (DE3). EPSPS was over-expressed in soluble form after induction with IPTG at 30 degrees...
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PMID: 17286049
PDF is available here.
PDF is available here.
FEMS Microbiology Letters (259)2 2006
Abstract
We have attempted to assess the gene expression profile throughout the Mycobacterium leprae genome using an array technique. Twelve highly expressed gene regions were identified that show an alteration in expression levels upon infection. Six of these were pseudogenes. Although M. leprae has an exce...
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PMID: 16734781
PDF is available here.
PDF is available here.
Plant Molecular Biology (61)1-2 2006
Abstract
We have successfully transferred the pol cytoplasm and Rfp from the amphidiploid B. napus to the diploid species B. rapa and generated a doubled haploid pol cytoplasm B. rapa population that segregates for the Rfp gene. This was achieved through interspecific crosses, in vitro rescue of hybrid embry...
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PMID: 16786306
PDF is available here.
PDF is available here.
Journal of Biological Chemistry (281)18 2006
Abstract
We describe the expression cloning of three novel GalA transferases from a 22-kb R. leguminosarum genomic DNA insert-containing cosmid (pSGAT). Two of these enzymes modify the substrate, Kdo2-[4'-(32)P]lipid IV(A) and its 1-dephosphorylated derivative on the distal Kdo residue, as indicated by mild...
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PMID: 16497674
PDF is available here.
PDF is available here.