Find your PDFs fast.
Welcome to the Pubget Medical Subject Headings (MESH) browser. Click on a topic or subtopic below to explore related papers. In the gold box is the most recent paper about the current MESH term, and below are 20 related papers.
F Factor (0):
Recent article
F Factor
Journal of Biological Chemistry (284)30 2009
Abstract
We performed a detailed thermodynamic analysis of CcdB binding to several fragments of gyrase A subunit (GyrA) that contain the CcdB-binding site. Binding of CcdB to the shorter fragments was studied directly by isothermal titration calorimetry. Its binding to the longer GyrA59 fragment in solution...
|
PMID: 19465484
PDF is available here.
PDF is available here.
| < More recent | Older article > |
Journal of Molecular Biology (385)1 2009
Abstract
We have applied electron cryo-microscopy and single-particle methods to solve the long-existing ambiguity in the packing geometry of F-pilin subunits. We show that the F-pilus has an entirely different symmetry from any of the known bacterial pili as well as any of the filamentous bacteriophages, wh...
|
PMID: 18992755
PDF is available here.
PDF is available here.
Molecular Microbiology (70)4 2008
Abstract
We have found that mutation of codon 340 (lysine to alanine) reduces ATP-dependent DNA binding > 100-fold and correspondingly diminishes SopA activities that depend on it: inhibition of polymer formation and persistence, stimulation of basal-level ATP hydrolysis and localization over the nucleoid. T...
|
PMID: 18826408
PDF is available here.
PDF is available here.
Journal of Bacteriology (190)22 2008
Abstract
We investigated the contribution of a natural IncF conjugative F plasmid to the formation of E. coli biofilms. Unlike the presence of a derepressed F plasmid, the presence of a natural IncF F plasmid promoted biofilm formation by generating the cell-to-cell mating F pili between pairs of F(+) cells...
|
PMID: 18790864
PDF is available here.
PDF is available here.
Molecular Microbiology (70)1 2008
Abstract
We present the crystal structure of the C-terminal tail of TraD bound to the TraM tetramerization domain, the first structural evidence of relaxosome-coupling protein interactions. The structure reveals the TraD C-terminal peptide bound to each of four symmetry-related grooves on the surface of the...
|
PMID: 18717787
PDF is available here.
PDF is available here.
Molecular Microbiology (67)3 2008
Abstract
The Escherichia coli CpxAR two-component signal transduction system senses and responds to extracytoplasmic stress. The cpxA101* allele was previously found to reduce F plasmid conjugation by post-transcriptional inactivation of the positive activator TraJ. Microarray analysis revealed upregulation...
|
PMID: 18069965
PDF is available here.
PDF is available here.
Abstract
We have previously reported the construction of the BAC clone (pRB-1B5) of the highly oncogenic Marek's disease virus (MDV) strain RB-1B, which has proven to be a valuable resource for elucidating several oncogenic determinants. Despite the retention of the BAC replicon within the genome, the recons...
|
PMID: 18230192
PDF is available here.
PDF is available here.
Abstract
We show that mutation of conserved arginine residues that map to the surface of Bacillus subtilis Soj caused only minimal effects on nucleotide-dependent dimerization but had dramatic effects on DNA binding. Using a model plasmid partitioning system in Escherichia coli, we find that Soj DNA-binding...
|
PMID: 18077387
PDF is available here.
PDF is available here.
Abstract
Risk of distal adenoma was significantly lower among subjects in high (approximately 5.7 ps/d) versus low (approximately 1.2 ps/d) quintiles of total fruit intake (OR: 0.75; 95% CI: 0.66, 0.86, P for trend <0.001), which was not completely explained by dietary folate or fiber intake. Inverse associa...
|
PMID: 18065596
PDF is available here.
PDF is available here.
Journal of Bacteriology (189)18 2007
Abstract
We investigated the structural and functional organization of the F plasmid conjugative coupling protein TraD by coimmunoprecipitation, cross-linking, and genetic means. We present direct evidence that coupling proteins form stable oligomeric complexes in the membranes of bacteria and that the forma...
|
PMID: 17631633
PDF is available here.
PDF is available here.
Abstract
I and IV in bovine, pig, and bird feces and from pig slurries. Both MST methods were applied to 28 water samples collected from three watersheds at different times. Classification of water samples as subject to human, animal, or mixed fecal contamination was more frequent when using Bacteroidales ma...
|
PMID: 17557850
PDF is available here.
PDF is available here.
Abstract
I and IV) fecal sources, in similar proportions to those obtained with a nucleic acid hybridization assay. This novel group-specific antibody-based particle agglutination technique for rapid and simple detection and grouping of F+ coliphages provides a new and improved tool for monitoring the microb...
|
PMID: 17483282
PDF is available here.
PDF is available here.
Molecular Microbiology (64)5 2007
Abstract
We simultaneously analysed movements of the plasmid and the SopA ATPase in single living cells. SopA, which is a putative motor protein assembled densely near nucleoid borders and formed a single discrete focus associated with less dense filamentous distribution along the long axis of the cell. The...
|
PMID: 17542915
PDF is available here.
PDF is available here.
FEMS Microbiology Letters (269)2 2007
Abstract
These results demonstrate the conjugative transfer of these elements to other bacterial species. Furthermore, it is reported that Ll.LtrB, a mobile group II intron carried by both elements, can invade its recognition site upon pRS01 conjugative transfer to E. faecalis....
|
PMID: 17263841
PDF is available here.
PDF is available here.
Microbiology (153)Pt 2 2007
Abstract
I to give a fragment called TraG* that is responsible for Mps. Using mutational analysis and cell localization studies, it was shown that TraG* is most probably an artifact caused by non-specific degradation. TraS (173 aa in F), which is involved in entry exclusion (Eex), blocks redundant conjugativ...
|
PMID: 17259615
PDF is available here.
PDF is available here.
Molecular Microbiology (63)4 2007
Abstract
Conjugative DNA transfer is a highly conserved process for the direct transfer of DNA from a donor to a recipient. The conjugative initiator proteins are key players in the DNA processing reactions that initiate DNA transfer - they introduce a site- and strand-specific break in the DNA backbone via...
|
PMID: 17238924
PDF is available here.
PDF is available here.
Abstract
It is well documented that microbial contamination of coastal waters poses a significant risk to human health through recreational exposure and consumption of shellfish. Identifying the source of microbial contamination (microbial source tracking) plays a dominant role in enabling effective manageme...
|
PMID: 17142373
PDF is available here.
PDF is available here.
Abstract
Synthetic Cys2His2 zinc finger domains with novel DNA-binding specificities can be identified from large randomized libraries using selection methodologies such as phage display. It has been previously demonstrated that a bacterial cell-based two-hybrid system is at least as effective as phage displa...
|
PMID: 18314590
PDF is available here.
PDF is available here.
Folia Microbiologica (52)5 2007
Abstract
Cell viability during chronological aging and after apoptotic stimuli in some yeast mutants with altered mitochondrial morphology was followed; a function for the corresponding genes in the apoptotic process was assessed. MDM30 and DNM1, the genes encoding an F-box protein and the dynamin-related GT...
|
PMID: 18298044
PDF is available here.
PDF is available here.
Molecular Microbiology (63)2 2007
Abstract
We have examined the effects of non-sequence-specific DNA on in vitro self-assembly of the SopA partition ATPase of plasmid F. SopA underwent polymerization provided ATP was present. DNA inhibited this polymerization and caused breakdown of pre-formed polymers. Centromere-binding protein SopB counte...
|
PMID: 17166176
PDF is available here.
PDF is available here.
Abstract
We demonstrated that the small plasmids below (20 kpb) are responsible for maintaining and dissemination of ESBL phenotype among the different species of Enterobacteriaceae family. The similarity of plazmid profiles isolated from E. coli and Klebsiella pneumoniae and K. oxytoca suggests possibility...
|
PMID: 18078117
PDF is available here.
PDF is available here.
Journal of Virological Methods (138)1-2 2006
Abstract
I and II were detected in all samples (n=7); GIII in six samples and GIV was never detected. GI was predominant in one sample, in which the quantity of Cryptosporidium and Giardia was, respectively, three and eight times higher than the mean values. Because GI is mainly observed in animals, it was h...
|
PMID: 16997389
PDF is available here.
PDF is available here.
Abstract
The large virulence plasmid pSFO157 of sorbitol-fermenting E. coli O157:H(-) strain 3072/96 has a size of 121,239bp and contains 96 open reading frames >50bp. It is therefore 29,162bp larger (ca. 32%) than plasmid pO157 of E. coli O157:H7 strain EDL933. Major differences between the plasmids are the...
|
PMID: 16887390
PDF is available here.
PDF is available here.
Low abundance of Escherichia coli microsatellites is associated with an extremely low mutation rate.
Abstract
We show that this high microsatellite instability depends on the presence of the F-plasmid. E. coli cells lacking the F-plasmid have extremely low microsatellite mutation rates. This result provides a possible explanation for the genome-wide low density of microsatellites in E. coli. Furthermore, we...
|
PMID: 16910996
PDF is available here.
PDF is available here.
Journal of Bacteriology (188)17 2006
Abstract
We report a mutagenesis of the F plasmid relaxase gene traI using in-frame, 31-codon insertions. Phenotypic analysis of our mutant library revealed that several mutant proteins are functional in conjugation, highlighting regions of TraI that can tolerate insertions of a moderate size. We also demons...
|
PMID: 16923902
PDF is available here.
PDF is available here.
FEMS Microbiology Letters (259)1 2006
Abstract
Escherichia coli TolA is a cytoplasmic membrane protein required for outer membrane integrity and the translocation of F-specific filamentous (Ff) bacteriophage DNA. Both phage infection and membrane integrity depend on several TolA interactions, e.g. those of the TolA C-terminal domain (TolAIII). M...
|
PMID: 16684106
PDF is available here.
PDF is available here.
Genetika (Moskva) (42)5 2006
Abstract
Mobilizable shuttle plasmids containing the origin of transfer (oriT) region of plasmid F (IncFI), ColIb-P9 (IncI1), and RP4/RP1 (IncPalpha) were constructed to test the ability of the cognate conjugation system to mediate gene transfer from Escherichia coli to Streptomyces. The conjugative system o...
|
PMID: 16808239
PDF is available here.
PDF is available here.
Abstract
We therefore isolated coliphages from municipal wastewater sludge and from biosolid samples after thermophilic anaerobic digestion to evaluate the susceptibility of specific groups to thermal inactivation. Similar numbers of F+ DNA and F+ RNA coliphages were found in untreated sludge, but the majori...
|
PMID: 16597945
PDF is available here.
PDF is available here.
Journal of Molecular Biology (356)4 2006
Abstract
We have analyzed by immunofluorescence microscopy the subcellular localization of SopA and SopB. The majority of SopB molecules formed foci, which localized frequently with F plasmid DNA molecules. The foci increased in number in proportion to the cell length. Interestingly, beside the foci formatio...
|
PMID: 16403518
PDF is available here.
PDF is available here.
Journal of Bacteriology (188)6 2006
Abstract
We know from previous work that in addition to promoting cell-to-cell contact, CluA is involved in sex factor DNA transfer. To define the CluA domains involved in aggregation and in transfer, we first performed random mutagenesis of the cluA gene using a modified mini-Tn7 element which generated fiv...
|
PMID: 16513740
PDF is available here.
PDF is available here.
BMC Microbiology (6)1 2006
Abstract
We performed a large-scale survey for genes on the E. coli chromosome that are affected by the presence of the conjugative F-plasmid (crosstalk). The expression pattern of about 4% (107 genes) of the genes encoded by the chromosome was affected by the presence of the F-plasmid. Comparing two differe...
|
PMID: 16981998
PDF is available here.
PDF is available here.
Nucleic Acids Research (34)2 2006
Abstract
Prior to conjugative transfer of plasmids, one plasmid strand is cleaved in a site- and strand-specific manner by an enzyme called a relaxase or nickase. In F and related plasmids, an inverted repeat is located near the plasmid strand cleavage site, and others have proposed that the ability of this...
|
PMID: 16418503
PDF is available here.
PDF is available here.
Journal of Bacteriology (188)2 2006
Abstract
We report that transcription from PY, the tra operon promoter, is repressed by the host nucleoid-associated protein, H-NS. Electrophoretic mobility shift assays indicate that H-NS binds preferentially to the tra promoter region, while Northern blot and transcriptional fusion analyses indicate that t...
|
PMID: 16385041
PDF is available here.
PDF is available here.
Journal of Bacteriology (188)1 2006
Abstract
We demonstrate that the host RNA binding protein, Hfq, represses TraJ and TraM synthesis by destabilizing their respective mRNAs. Mating assays and immunoblot analyses for TraM and TraJ showed that transfer efficiency and protein levels increased in host cells containing a disruption in hfq compared...
|
PMID: 16352828
PDF is available here.
PDF is available here.
Journal of Bacteriology (187)24 2005
Abstract
Our results indicate that TrbB(F), DsbC(R27), and HtdT(R27) are putative disulfide bond isomerases for their respective transfer systems. TraF(F) is essential for conjugation but appears to have a function other than disulfide bond chemistry....
|
PMID: 16321931
PDF is available here.
PDF is available here.
Abstract
Microbiology (151)Pt 11 2005
Abstract
I abolished filament formation as measured by fluorescent R17 binding. This was confirmed by measurements of DNA donor activity and filamentous DNA bacteriophage infection. With one exception (G53C), cysteines elsewhere in the F-pilin primary structure did not abolish filament formation, although so...
|
PMID: 16272377
PDF is available here.
PDF is available here.
Microbiology (151)Pt 11 2005
Abstract
F plasmid TraN (602 aa, processed to 584 aa with 22 conserved cysteines), which is essential for F plasmid conjugation, is an outer-membrane protein involved in mating pair stabilization (MPS). Unlike R100 TraN, F TraN requires OmpA in the recipient cell for efficient MPS. The authors have identifie...
|
PMID: 16272376
PDF is available here.
PDF is available here.
Abstract
The TraI protein of conjugative plasmid F factor binds and cleaves a single-stranded region of the plasmid prior to transfer to a recipient. TraI36, an N-terminal TraI fragment, binds ssDNA with a subnanomolar K(D) and remarkable sequence specificity. The structure of the TraI36 Y16F variant bound t...
|
PMID: 16216584
PDF is available here.
PDF is available here.
Abstract
We characterized in vitro the interactions of the host DnaA initiation protein and DnaB helicase from Pseudomonas aeruginosa and Pseudomonas putida with the replication origin, oriS, and initiation protein, RepE, of the RepFIA replicon. The results presented here show that a pre-priming complex can...
|
PMID: 15907538
PDF is available here.
PDF is available here.
Journal of Bacteriology (187)14 2005
Abstract
We present evidence that F conjugation also requires TraM-TraD interactions in vivo. A three-plasmid system was used to select mutations in TraM that are defective for F conjugation but competent for tetramerization and cooperative DNA binding to the traM promoter region. One mutation, K99E, was par...
|
PMID: 15995191
PDF is available here.
PDF is available here.
Molecular Microbiology (56)6 2005
Abstract
We analyse the mechanism of partition-associated incompatibility for plasmid pB171, a phenotype associated with all known plasmid-encoded centromere loci. An R1 plasmid carrying par2 from plasmid pB171 was destabilized by the presence of an F plasmid carrying parC1, parC2 or the entire par2 locus of...
|
PMID: 15916596
PDF is available here.
PDF is available here.
Abstract
The importance of conjugation as a mechanism to spread biofilm determinants among microbial populations was illustrated with the gram-positive bacterium Lactococcus lactis. Conjugation triggered the enhanced expression of the clumping protein CluA, which is a main biofilm attribute in lactococci. Cl...
|
PMID: 15932992
PDF is available here.
PDF is available here.
Journal of Molecular Biology (348)5 2005
Abstract
Gyrase is an ubiquitous bacterial enzyme that is responsible for disentangling DNA during DNA replication and transcription. It is the target of the toxin CcdB, a paradigm for plasmid addiction systems and related bacterial toxin-antitoxin systems. The crystal structure of CcdB and the dimerization...
|
PMID: 15854646
PDF is available here.
PDF is available here.
Abstract
Analystic test for nucleic acid revealed that the two phages both belonged to 6000 bp, single-stranded RNA bacteriophage. Significant differences in their specificity of RAPD-PCR and RT-PCR were found during the changed of host range; with 26 RAPD-cDNA differential fragments found that in two phages...
|
PMID: 16053763
PDF is available here.
PDF is available here.
Microbiology (151)Pt 4 2005
Abstract
The antibiotic-multiresistance IncF plasmid pRSB107 was isolated by a transformation-based approach from activated-sludge bacteria of a wastewater-treatment plant. It confers resistance to ampicillin, penicillin G, chloramphenicol, erythromycin, kanamycin, neomycin, streptomycin, sulfonamides, tetra...
|
PMID: 15817778
PDF is available here.
PDF is available here.
Journal of Bacteriology (187)3 2005
Abstract
We report the dissemination of the Ll.LtrB group II intron among L. lactis strains following conjugative transfer of the native chromosomally embedded L. lactis sex factor. We demonstrated that Ll.LtrB dissemination is highly variable and often more efficient from this integrative and conjugative el...
|
PMID: 15659671
PDF is available here.
PDF is available here.
Journal of Bacteriology (187)4 2005
Abstract
We genetically screened for E. coli chromosomal mutations that influenced P1 stability and identified a novel temperature-sensitive allele of the dnaB helicase gene (dnaB277) that replaces serine 277 with a leucine residue (DnaB S277L). This allele conferred a severe temperature-sensitive phenotype...
|
PMID: 15687186
PDF is available here.
PDF is available here.
Abstract
Hfr x F- recombinational genome interactions are simulated by a computer program. Genotype spectrums of selected samples from Hfr x F- crosses of Escherichia coli, found in the published literature, are reproduced. Relevance of the findings for interpretation of conjugational results in Escherichia...
|
PMID: 16400873
PDF is available here.
PDF is available here.
Molecular Microbiology (55)2 2005
Abstract
We have investigated the basis of incompatibility exerted by the F plasmid centromere, sopC, to probe the mechanism of partition. Measurements of the effects of sopC at various gene dosages on destabilization of mini-F, on repression of the sopAB operon and on occupancy of mini-F DNA by the centrome...
|
PMID: 15659167
PDF is available here.
PDF is available here.
FEMS Microbiology Letters (242)1 2005
Abstract
The ~93-kDa surface layer protein SgsE of Geobacillus stearothermophilus NRS 2004/3a forms a regular crystalline array providing a nanopatterned matrix for the future display of biologically relevant molecules. Lactococcus lactis NZ9000 was established as a safe expression host for the controlled ta...
|
PMID: 15675069
PDF is available here.
PDF is available here.
Journal of Bacteriology (187)2 2005
Abstract
We conclude that TraIDelta252 lacks an essential C-terminal domain that is required for DNA transfer. We speculate this domain may be involved in essential protein-protein interactions with other components of the DNA transfer machinery....
|
PMID: 15629940
PDF is available here.
PDF is available here.
Journal of Biological Chemistry (279)53 2004
Abstract
We used a two-plasmid system to screen for autoregulation-defective traM mutants generated by random PCR mutagenesis. A total of 72 missense mutations in TraM affecting autoregulation were selected, all of which also resulted in a loss of TraM function during F conjugation. Mutational analysis of Tr...
|
PMID: 15509578
PDF is available here.
PDF is available here.
Molecular Microbiology (54)3 2004
Abstract
We show that the nucleoid-associated host protein, H-NS, acts to repress the expression of traM and traJ as cells enter stationary phase, thereby decreasing mating ability to barely detectable levels. Sequence analysis identified regions of predicted intrinsic curvature, to which H-NS preferentially...
|
PMID: 15491366
PDF is available here.
PDF is available here.
Journal of Bacteriology (186)20 2004
Abstract
We present in vitro and in vivo data that demonstrate specific binding of CP TraD of the IncFII R1 plasmid transfer system to relaxosomal protein TraM. With overlay assays and enzyme-linked immunosorbent assays we showed that a truncated version of TraD, termed TraD11 (DeltaN155), interacted strongl...
|
PMID: 15466052
PDF is available here.
PDF is available here.
Abstract
The F plasmid PifA protein, known to be the cause of F exclusion of bacteriophage T7, is shown to be a membrane-associated protein. No transmembrane domains of PifA were located. In contrast, T7 gp1.2 and gp10, the two phage proteins that trigger phage exclusion, are both soluble cytoplasmic protein...
|
PMID: 15302217
PDF is available here.
PDF is available here.
Microbiology (150)Pt 8 2004
Abstract
CluA is a 136 kDa surface-bound protein encoded by the chromosomally located sex factor of Lactococcus lactis MG1363 and is associated with cell aggregation linked to high-frequency transfer of the sex factor. To further investigate the involvement of CluA in these phenomena, the cluA gene was clone...
|
PMID: 15289547
PDF is available here.
PDF is available here.
Journal of Bacteriology (186)16 2004
Abstract
We have defined an interaction group of six Tra proteins encoded by the F plasmid and required by F(+) cells to elaborate F pili. The six proteins are TraH, TraF, TraW, TraU, TrbI, and TrbB. Except for TrbI, these proteins were all identified as hallmarks of F-like type IV secretion systems (TFSSs),...
|
PMID: 15292150
PDF is available here.
PDF is available here.
Journal of Bacteriology (186)16 2004
Abstract
We show here that this sensitivity has two components. The first derives from secretion of bacteriophage particles through the cell envelope, but the second can be attributed to expression of the F genes required for the formation of conjugative (F) pili. The latter component was manifested as reduc...
|
PMID: 15292121
PDF is available here.
PDF is available here.
Journal of Biological Chemistry (279)28 2004
Abstract
We examined the temperature, salt, and pH dependence of TraI36 recognition. Binding is entropically driven below 25 degrees C and enthalpically driven at higher temperatures. van't Hoff analysis yields an estimated deltaC(P)(0) of binding (-3300 cal x mol(-1) x K(-1)) that is larger and more negativ...
|
PMID: 15123728
PDF is available here.
PDF is available here.