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Hemolysin Factors (0):
Recent article
Hemolysin Factors
[Influence of Lactobacillus fermentum metabolites on ultrastructure of pathogenic Escherichia coli].
Abstract
Changes pointing to profound destructive processes in bacterial cells were detected on ultrathin sections. Under the influence of diffusing metabolites of lactobacilli, the following changes were observed: destabilization of cell wall, expansion of periplasmatic space, and emergence of low electron...
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PMID: 19459468
PDF is available here.
PDF is available here.
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Microbiology (153)Pt 4 2007
Abstract
The sequencing of prfA, encoding the transcriptional regulator of virulence genes, in 26 low-virulence field Listeria monocytogenes strains showed that eight strains exhibited the same single amino-acid substitution: PrfAK220T. These strains exhibited no expression of PrfA-regulated proteins and thu...
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PMID: 17379709
PDF is available here.
PDF is available here.
Abstract
Uropathogenic Escherichia coli strains isolated from individuals living in different areas of the city of Rio de Janeiro were characterized according to serotype, hemolytic properties, hemagglutination properties, antimicrobial susceptibility and isoenzymatic profile. The molecular approach used, to...
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PMID: 17308707
PDF is available here.
PDF is available here.
FEMS Microbiology Letters (99)2-3 2006
Abstract
Eight hundred and nineteen strains of Escherichia coli isolated in Spain between 1986 and 1991 from extraintestinal infections and feces of healthy controls were investigated for expression of P-fimbriae using a particle agglutination test. Among strains causing urinary tract infections, sepsis and o...
PDF is available here.
PDF is available here.
Abstract
A retrospective study was conducted to determine case histories, microbiological characteristics, and molecular subtypes associated with Listeria monocytogenes infections of the eye in large animals. For selected cases, environmental L. monocytogenes contamination patterns on case farms were also ev...
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PMID: 15460335
PDF is available here.
PDF is available here.
Microbiology (147)Pt 5 2001
Abstract
We also identified one ribotype (DUP-1030) which was significantly more common among animal isolates (P=0.005) and one ribotype (DUP-1038; lineage I) which was significantly more common among human epidemic isolates as compared to human sporadic isolates (P<0.001). These findings confirm the presenc...
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PMID: 11320113
PDF is available here.
PDF is available here.
Infection and Immunity (68)6 2000
Abstract
We demonstrate that human monocyte-derived immature DCs can efficiently phagocytose L. monocytogenes. This uptake is independent of listerial adhesion factors internalin A and internalin B but requires cytoskeletal motion and factors present in human plasma. A major portion of internalized bacteria...
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PMID: 10816528
PDF is available here.
PDF is available here.
Molecular Microbiology (36)2 2000
Abstract
We previously described an unknown proteinaceous component from crude bacterial cell extracts, which, together with PrfA, formed a specific complex (CI) in electrophoretic mobility shift assays (EMSA) with an hly promoter probe. Here we identify the RNA polymerase (RNAP) of L. monocytogenes as an es...
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PMID: 10792734
PDF is available here.
PDF is available here.
Molecular Microbiology (30)2 1998
Abstract
The Enterococcus faecalis haemolysin plasmid pAD1 (60 kb) confers a conjugative mating response to an octapeptide sex pheromone (cAD1) secreted by plasmid-free strains. The response involves two plasmid-borne regulatory determinants: traE1, whose product positively regulates all or most conjugation-...
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PMID: 9791182
PDF is available here.
PDF is available here.
Infection and Immunity (66)1 1998
Abstract
Listeria monocytogenes infection of endothelial cells upregulates surface expression of adhesion molecules and stimulates neutrophil adhesion to infected cell monolayers. The experiments presented here tested the roles of specific bacterial virulence factors as triggers for this inflammatory phenoty...
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PMID: 9423863
PDF is available here.
PDF is available here.
Molecular Microbiology (20)4 1996
Abstract
We constructed and analysed substitutions of two amino acids of PrfA predicted to contact DNA, i.e. Ser-184 and Ser-183. Substitution of Ser-184 by Ala reduced DNA binding and virulence-gene activation, and attenuated the virulence in a mouse model of infection. In contrast, substitution of Ser-183...
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PMID: 8793875
PDF is available here.
PDF is available here.
Abstract
Exosubstances (cohemolysins) produced by Streptococcus agalactiae (CAMP-factor) and Streptococcus uberis (Uberis-factor) showing hemolytic synergism with beta-lysin produced by Staphylococcus aureus were compared. Cohemolytic activity was evaluated in the supernatants of bacterial cultures, before a...
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PMID: 8745004
PDF is available here.
PDF is available here.
Abstract
Twenty seven Aeromonas strains (5A. hydrophila, 8A. sobria and 14A. caviae) isolated from children with diarrhoea and 34 Aeromonas strains (9A. hydrophila, 7A. sobria an 18A. caviae) isolated from children without diarrhoea were tested from haemolysin production. The results obtained showed that hae...
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PMID: 9074783
PDF is available here.
PDF is available here.
FEMS Microbiology Letters (122)1-2 1994
Abstract
Erythromycin-resistant derivatives of the pheromone-responsive plasmid pIP964 from Enterococcus faecalis were constructed to study its host range. This was done by inserting the integrative vector pAT112 and the related replicon pTCR1 harboring oriR of the broad host range plasmid pAM beta 1 into th...
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PMID: 7958769
PDF is available here.
PDF is available here.
Abstract
I footprinting. Visualization of the complexes confirmed the specificity and metal-dependence of Fur binding and demonstrated that the protein polymerizes on its binding sites. Such a polymerization could be involved in the repression process of the bacterial regulator....
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PMID: 8198594
PDF is available here.
PDF is available here.
Epidemiology and Infection (112)2 1994
Abstract
Restriction fragment length polymorphism of ribosomal RNA genes was analysed among 133 Escherichia coli strains predominantly from blood and urine, including 21 isolates from faeces of healthy persons. The strains had also been characterized for their O:K:H serotypes, for the presence of P, S and ty...
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PMID: 7512044
PDF is available here.
PDF is available here.
Abstract
We report the nucleotide sequence of traB and the position of several transposon insertions resulting in the characteristic self-induction phenotype. The deduced product has a mass of 43.7 kDa with the C-terminal third consisting primarily of hydrophobic amino acid residues....
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PMID: 8029329
PDF is available here.
PDF is available here.
Abstract
A collection of 363 V. cholerae strains isolated from different sources were studied by the spectrum of their hemolytic activity in combination with biovar-associated properties. The strains were analyzed for the presence of the cholera toxin (CT) gene (vct) and the hemolysin gene (hly) with the use...
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PMID: 8017123
PDF is available here.
PDF is available here.
Pathologie Biologie (41)9 1993
Abstract
Journal of Bacteriology (175)16 1993
Abstract
pAD1, a conjugative, 60-kb, hemolysin-bacteriocin plasmid in Enterococcus faecalis, encodes a mating response to a small peptide sex pheromone, cAD1, secreted by potential recipient bacteria. A gene, traC, encoding a 60.7-kDa protein with a typical amino terminal signal peptide, was identified withi...
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PMID: 8349566
PDF is available here.
PDF is available here.
FEMS Microbiology Letters (78)2-3 1992
Abstract
Eight hundred and nineteen strains of Escherichia coli isolated in Spain between 1986 and 1991 from extraintestinal infections and feces of healthy controls were investigated for expression of P-fimbriae using a particle agglutination test. Among strains causing urinary tract infections, sepsis and...
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PMID: 1362709
PDF is available here.
PDF is available here.
Abstract
Beta-hemolysin production is a variable trait of the Lancefield group D streptococcus, Enterococcus faecalis. The E. faecalis hemolysin is encoded by large transmissible plasmids. The variable nature of this putative virulence factor provided an ideal system for testing its contribution in experimen...
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PMID: 1559764
PDF is available here.
PDF is available here.
Nucleic Acids Research (19)12 1991
Abstract
Abstract
A significant difference was observed in the occurrence of the examined markers (Col+, ColV+, Hly+, Aer+, AbR) and in the plasmid carrier state between strains with and without K1 and K5 antigens. Plasmids of the same size were harboured by serotypes possessing K1 and K5 antigens, e.g. among O1: K1:...
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PMID: 1810113
PDF is available here.
PDF is available here.
Abstract
The molecular organization of the alpha-hemolysin gene from Staphylococcus aureus strain O15 has been studied. Hybridization of the DNA from this strain with the [32P]-labeled cloned fragment containing alpha-hemolysin gene has shown the studied gene to be unique in the genome of the strain. Sequenc...
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PMID: 2366773
PDF is available here.
PDF is available here.
Abstract
We placed the cyaA gene and a truncated cyaA gene that lacks the nucleotides that code for a putative C-terminal secretory signal sequence under the control of the lac promoter in the plasmid pUC-19. These plasmids were transformed into strains of E. coli which contained the hly operon. The truncate...
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PMID: 2182114
PDF is available here.
PDF is available here.
Microbiology and Immunology (34)10 1990
Abstract
A DNA fragment encoding a hemolytic factor was cloned from the parasitic spirochete Leptospira interrogans serovar autumnalis strain Congo 21-543. Initial clones were isolated by screening a genomic library in pBR322 in Escherichia coli for hemolytic activity. Hemolytic activity was coded by a 4.5 k...
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PMID: 2077368
PDF is available here.
PDF is available here.
Cloning and characterization of a hemolysin gene from Actinobacillus (Haemophilus) pleuropneumoniae.
Abstract
We designate appC and appA, respectively, are similar in sequence to the hlyCA genes of Escherichia coli and the lktCA genes of P. haemolytica. Hemolytic activity could be detected in lysates of E. coli harboring plasmids containing the appCa genes....
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PMID: 2693022
PDF is available here.
PDF is available here.
Abstract
We have determined the DNA sequences of eight different insertions of IS91 in a specifically engineered recipient plasmid of known DNA sequence (pSU300). The sequences at the termini of IS91 are 5'-CGAGTAGG...CCTATCGAT. IS91 inserts specifically 5' to either one of the tetranucleotides 5'-GAAC or 5'...
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PMID: 2552258
PDF is available here.
PDF is available here.
Abstract
We summarize what is known about the biochemistry and genetics of this phenomenon. Some emphasis is given to the hemolysin plasmid pAD1 and the regulation of its conjugal transfer....
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PMID: 2550976
PDF is available here.
PDF is available here.
Abstract
Our experiments confirmed the suggestion of Smith that in vivo transfer of plasmid in the intestine of animals is only possible when the donor transfers the plasmid with high frequency and readily colonizes the intestine. The pHly::Tn5 plasmid acquired by in vitro recombination does not spread with...
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PMID: 2550170
PDF is available here.
PDF is available here.
Abstract
The HlyA protein (Mr 110 kDa) which is the gene product of the hlyA gene encoded by the hemolysin determinant of Escherichia coli (Goebel, W. & Hedgpeth, J. (1982) J. Bacteriol. 151, 1290-1298) was observed to accumulate in the culture supernatant (in the presence of the three other Hly proteins Hly...
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PMID: 3279976
PDF is available here.
PDF is available here.
Zentralblatt für Bakteriologie, Mikrobiologie und Hygiene. Series A: Medical Microbiology, Infectious Diseases, Virology, Parasitology (266)1-2 1987
Abstract
These results indicate that the extracellular haemolytic activity encoded by certain hly-plasmids was controlled by the chromosomally encoded fur gene....
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PMID: 3321765
PDF is available here.
PDF is available here.
Abstract
Data on the hemolysin synthesis determined by the plasmid pHly241, belonging to the incompatibility group I2, and its derivatives are presented in this paper. The restriction analysis of the pHly241 plasmid has resulted in the detailed mapping of the hly determinant region in the plasmid. The substa...
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PMID: 3033483
PDF is available here.
PDF is available here.
Abstract
Abstract
Abstract
A basic hypothesis running through all of these possible future approaches to the treatment of urinary tract infections is the concept that these infections are first initiated by bacterial adherence to mucosal surfaces in the lower urinary tract and that therapy might be devised to lessen this adhe...
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PMID: 3535215
PDF is available here.
PDF is available here.
Zentralblatt für Bakteriologie, Mikrobiologie und Hygiene. Series A: Medical Microbiology, Infectious Diseases, Virology, Parasitology (261)4 1986
Abstract
1818 E. coli wild-type strains from patients of the region of Berne were screened for their haemolytic property and for carriage of hly-plasmids. Among 885 strains of fecal and 993 strains of urinary tract isolates 136 (15%) and 259 (28%) were haemolytic respectively. In five fecal, but none of the...
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PMID: 3020844
PDF is available here.
PDF is available here.
Abstract
The data obtained indicate that pAP20 plasmid determinants for the synthesis and excretion of Escherichia coli haemolysin are located on the plasmid SalI-fragment f3. The size of f3 fragment is 12 X 10(6) dalton.
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PMID: 3011146
PDF is available here.
PDF is available here.
Abstract
Like all other Salmonella typhimurium strains examined, the smooth variants SF1397 (LT2) and 1366 and also their semi-rough and rough derivatives are non-haemolytic. Nevertheless, two haemolysin (Hly) plasmids of E. coli belonging to the inc groups incFIII,IV (pSU316) and incI2 (pHly152) were able t...
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PMID: 2413797
PDF is available here.
PDF is available here.
Abstract
E. coli strains isolated from urinary specimens of patients suffering from pyelonephritis can be defined as uropathogenic by the following entities: production of hemolysins, adhesins and hydroxamates, serum resistance, capsule formation and specific O: H antigenes. The presence of these properties...
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PMID: 6385544
PDF is available here.
PDF is available here.
Journal of Bacteriology (159)1 1984
Abstract
The hemolysin plasmid pCW2, which enhances the virulence of Escherichia coli, has been mapped. Comparison of the region coding for hemolysin with other hemolysin determinants reveals differences that could explain differences in the ability to confer virulence.
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PMID: 6330049
PDF is available here.
PDF is available here.
Microbiologica (7)2 1984
Abstract
We could also isolate three different types of transconjugants in hemolytic activity. The interpretation of this observation was facilitated by the research of bacteriocin activity. We also classified the bacteriocins found in our strains into different types....
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PMID: 6431229
PDF is available here.
PDF is available here.
Abstract
Wild-type strain A454 (Streptococcus pyogenes) transferred en bloc its erythromycin (Em) and tetracycline (Tc) resistance markers into several plasmid-free streptococcal recipients. No plasmid DNA was detected in either the wild-type or the transconjugant strains. Crosses were performed between A454...
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PMID: 6330497
PDF is available here.
PDF is available here.
Abstract
We have recently reported the secretion of a 107K polypeptide by an E. coli strain containing the haemolytic plasmid pHly167 (Mackman and Holland 1984). In this paper we show that a large number of haemolytic E. coli strains, apparently including both plasmid and chromosomally located haemolysin gen...
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PMID: 6090863
PDF is available here.
PDF is available here.
Microbiologica (7)1 1984
Abstract
Seven strains of Streptococcus faecalis subsp. zymogenes were studied. Plasmids originally harboured by these bacteria, codifing for drug resistance, hemolysin and gelatinase activity, were transferred to a recipient strain ( JH2 -2) by conjugation. Studies on bacteria in mating showed the formation...
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PMID: 6328222
PDF is available here.
PDF is available here.
Infection and Immunity (42)1 1983
Abstract
After intraperitoneal injection of mice with Escherichia coli strains isolated from patients with urinary tract infections, the mortality due to hemolytic (Hly+) and nonhemolytic (Hly-) isolates was 77 and 40%, respectively. Deletion of the chromosomal hemolysin (hly) determinant in an E. coli O6:K1...
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PMID: 6352503
PDF is available here.
PDF is available here.
Infection and Immunity (41)3 1983
Abstract
These results strongly suggest that there is a unique ubiquitous genetic determinant responsible for the hemolytic phenotype in E. coli....
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PMID: 6350187
PDF is available here.
PDF is available here.
Infection and Immunity (41)3 1983
Abstract
A DNA sequence encoding Staphylococcus aureus alpha-hemolysin, which had been previously cloned and mapped in Escherichia coli K-12, was introduced into Bacillus subtilis BD170 and several strains of S. aureus by using plasmid vectors, some of which could replicate in all three organisms. The determ...
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PMID: 6411618
PDF is available here.
PDF is available here.
Infection and Immunity (41)3 1983
Abstract
A fragment of Staphylococcus aureus DNA encoding the alpha-hemolysin determinant was cloned from strain Wood 46 by inserting Sau3A-generated genomic DNA fragments between the BamHI sites of the lambda replacement vector L47.1. Phages expressing alpha-hemolysin were detected by overlaying plaques for...
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PMID: 6350179
PDF is available here.
PDF is available here.
Abstract
Experiments on isogenic strains of different enterobacterial species, both carrying hemolytic plasmids and free from them, have demonstrated that their "killing" effect on mice is linked with the yield of hemolysin and does not depend on the origin of Hly plasmids or the chemotype of bacteria. Plasm...
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PMID: 6356727
PDF is available here.
PDF is available here.
Abstract
The haemolytic plasmid pSU316 is incompatible with members of the IncFIII and IncFIV incompatibility groups. Plasmid pSU307 (pSU316 hlyC::Tn5) was inserted by integrative suppression into the chromosome of JW112, a temperature-sensitive dnaA mutant of Escherichia coli. The incompatibility properties...
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PMID: 6313856
PDF is available here.
PDF is available here.
Journal of Bacteriology (154)3 1983
Abstract
We determined that a related sequence flanks also the hlyB-distal end of the chromosomal hly determinant of E. coli 536. In addition several other similar or even identical sequences are found in the vicinity of the hlyC- and the hlyB-distal ends of both the chromosomal and the plasmid hly determina...
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PMID: 6343344
PDF is available here.
PDF is available here.
Abstract
The replication and incompatibility region of the IncFVI plasmid pSU502 has been isolated by in vitro DNA manipulation as part of a 12.6 kb plasmid, denominated pSU503. Plasmid pSU503 was strongly incompatible with its parental plasmid, pSU1, but was fully compatible with the haemolytic plasmids pSU...
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PMID: 6308130
PDF is available here.
PDF is available here.
Antibiotiki (28)2 1983
Abstract
Abstract
The hemolytic phenotype in Escherichia coli is determined by four genes. Two (hlyC and hlyA) determine the synthesis of a hemolytically active protein which is transported across the cytoplasmic membrane. The other two genes (hlyBa and hlyBb) encode two proteins which are located in the outer membra...
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PMID: 6368575
PDF is available here.
PDF is available here.
Journal of Bacteriology (152)2 1982
Abstract
A nonconjugative hemolysin plasmid, pHly185, was isolated from Escherichia coli 185. Tn5 mutagenesis produced nonhemolytic derivatives that either expressed no hemolytic activity or did not secrete activity. These Tn5 insertions were located in three contiguous EcoRI fragments. Insertions inactivati...
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PMID: 6215398
PDF is available here.
PDF is available here.
Journal of Bacteriology (151)1 1982
Abstract
Alpha-hemolytic plasmids belonging to four different incompatibility groups contained several copies (two to six) of the same DNA sequence. This sequence was capable of illegitimate recombination and thus behaved as an insertion sequence element. Its size (1.9 kilobases) and restriction enzyme cleav...
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PMID: 6282809
PDF is available here.
PDF is available here.
Infection and Immunity (35)1 1982
Abstract
The nephropathogenic Escherichia coli strain P673 was shown to harbor two plasmids with molecular sizes of 70 and 41 megadaltons, respectively. The 70-megadalton plasmid, pCW1, coded for tetracycline resistance, whereas hemolysin production was coded by the 41-megadalton plasmid, pCW2. Plasmid pCW1...
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PMID: 7033139
PDF is available here.
PDF is available here.
Infection and Immunity (35)1 1982
Abstract
The sensitivity to normal human serum of 91 smooth strains of Escherichia coli isolated from urinary tract infections was determined. Production of hemolysin, which was common and associated primarily with the types O4, O6, O18, and O75, was significantly correlated with high levels of serum resista...
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PMID: 7033137
PDF is available here.
PDF is available here.