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Lactose Factors (0):
Recent article
Lactose Factors
Mutation Research (419)1-3 1998
Abstract
These results suggest that the transgenic rat model is a useful tool for studying potential genotoxicity in target organs and, with further validation, the selectable cII target could be an attractive alternative to the conventional lacI color screening method for the detection of mutations in the l...
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PMID: 9804924
PDF is available here.
PDF is available here.
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Abstract
We have exploited this reaction to develop a method for removal of polymerase-produced mutant sequences that arise during sequence amplification by PCR. After denaturation and reannealing, the PCR product pool is subjected to MutH, MutL, and MutS mismatch repair proteins under double-strand cleavage...
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PMID: 9192654
PDF is available here.
PDF is available here.
Neuroscience (78)3 1997
Abstract
We pursued a strategy of transferring a potentially neuroprotective gene into rat embryonic mesencephalic rat cells in vitro, before grafting them into the denervated striatum of 6-hydroxydopamine-lesioned rats. We performed intrastriatal grafts of embryonic day 14 mesencephalic cells infected with...
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PMID: 9153652
PDF is available here.
PDF is available here.
Abstract
We have identified two distinct subsets of primary sensory olfactory neurons (PONs) in the H-OMP-LacZ-6 transgenic mouse. In these transgenic mice, a LacZ reporter gene under the control of a 294 base pair element from the 5' promoter region of the olfactory marker protein (OMP) gene was expressed i...
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PMID: 8731225
PDF is available here.
PDF is available here.
Abstract
We examine the deaminating ability of aerobic nitric oxide (NO) toward single-stranded DNA at very low (micromolar and below) total exposures, using a sensitive genetic method that allows us to study a single deamination event at a specific site in a 7200-nucleotide DNA molecule within a pool of ca....
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PMID: 8828926
PDF is available here.
PDF is available here.
Abstract
A novel insertion sequence, designated IS1070, was identified on the lactose plasmid of Leuconostoc lactis NZ6009 by nucleotide sequence analysis. The 1027-bp sequence contains partially matched (24 of 28 bp) inverted repeats and has one long open reading frame. The deduced 305-amino-acid sequence d...
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PMID: 7698675
PDF is available here.
PDF is available here.
Abstract
The structure difference between the free operator of the lac system d(GCTCACAAT).d(ATTGTGAGC) and the same operator complexed to the headpiece of the lac repressor has been investigated by 2-D-1H NMR spectroscopy in conjunction with molecular modelling in internal coordinates (JUMNA). The free and...
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PMID: 8043650
PDF is available here.
PDF is available here.
Abstract
Expression of the LAC9 gene from the yeast Kluyveromyces lactis in HepG2 human hepatoblastoma cells efficiently induced luciferase expression from reporter plasmids containing the four LAC9 binding sites from the K. lactis GAL1-GAL10 gene linked to a basal promoter. Induction was approximately 100fo...
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PMID: 8338633
PDF is available here.
PDF is available here.
Molecular and General Genetics (238)1-2 1993
Abstract
We set up a system to re-examine this issue. We analysed the ability of mutant CAP-E171L and CAP-E171K proteins to bind a near-consensus CAP site in vivo and found it to be diminished fourfold relative to wild type in each case. Activation of lac transcription by these mutant proteins remains the sa...
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PMID: 8097556
PDF is available here.
PDF is available here.
Molecular Microbiology (6)21 1992
Abstract
High-frequency conjugation of the lactose plasmid pLP712 is associated with a constitutive cell aggregation phenotype and is facilitated by cointegration with a sex factor. Analysis of 23 independently derived enlarged lactose plasmids revealed that the sex factor DNA present in cointegrates varied...
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PMID: 1453959
PDF is available here.
PDF is available here.
Journal of Molecular Biology (219)4 1991
Abstract
Escherichia coli lac repressor is a tetrameric protein composed of 360 amino acid subunits. Considerable attention has focused on its N-terminal region which is isolated by cleavage with proteases yielding N-terminal fragments of 51 to 59 amino acid residues. Because these short peptide fragments bi...
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PMID: 1905359
PDF is available here.
PDF is available here.
Abstract
A number of plasmids carrying the Lac+ character have been reported. Lac+ character of salmonella S:41:z10:- studied for transfer of Lac+ character to standard Escherichia coli K12 Lac-F- Nalr and Escherichia coli K12 F- Lac- Rifr, failed to transfer in in vitro experiments. Similarly, identificatio...
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PMID: 1803005
PDF is available here.
PDF is available here.
Abstract
A 4.4-kb EcoR1-EcoR1 DNA fragment from the Lactococcus lactis subsp. cremoris plasmid pDI-21 encoded the tagatose 1,6-bisphosphate (TBP) aldolase gene and the Lac-PTS genes. In vitro transcription-translation using Escherichia coli S30 extract showed the synthesis of 41,000-, 23,000- and 12,000-dalt...
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PMID: 1367486
PDF is available here.
PDF is available here.
Journal of Biological Chemistry (265)14 1990
Abstract
These results suggest that the resistance of the elongation complex to these antibodies is not a consequence of their inability to bind to RNA polymerase. Only one of the alpha subunits may be involved in the initial process of transcription, and the antigenic domain of this subunit appears to be oc...
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PMID: 2186035
PDF is available here.
PDF is available here.
Genetics Research (54)2 1989
Abstract
The chromosomal lac region of the coliform bacterium Klebsiella M5al was cloned into the multicopy plasmid pBR322 to give pHE7 and pHE8. pHE8 contains 12.6 kb of M5al DNA, including its complete lac operon, and pHE7 contains 2.5 kb of M5al DNA and includes the complete lac Y gene and a small segment...
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PMID: 2515112
PDF is available here.
PDF is available here.
Abstract
The Lactococcus lactis subsp. lactis KP3 Lac genetic element was investigated. KP3 is a lactose-positive (Lac+) transconjugant which contains no detectable plasmid DNA. The KP3 Lac genetic element was self-transmissible (Tra+) and encoded a reduced bacteriophage sensitivity (Rbs+) phenotype. Matings...
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PMID: 2506593
PDF is available here.
PDF is available here.
Abstract
A simple method for curing plasmid DNA from lactic streptococci is described. When strains of lactic streptococci are grown overnight at 32 degrees C in an unbuffered medium (M17-) and held at the same temperature for an extended period (96 h), the acid environment induces loss of plasmid DNA of dif...
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PMID: 2498159
PDF is available here.
PDF is available here.
Abstract
Lactose fermenting Salmonella typhimurium are endemic in São Paulo, but not in Rio de Janeiro Two isolations are described from the latter city. These Rio de Janeiro strains have a plasmid of 7.4 megadaltons. These plasmids were not auto-transferable, were thermostable and were not eliminated by ac...
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PMID: 2700499
PDF is available here.
PDF is available here.
Journal of Molecular Biology (203)3 1988
Abstract
We conclude that UmuC function, rather than being essential, facilitates recovery and specifically, following the model of Bridges & Woodgate, that it facilitates the prompt resumption of chain elongation....
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PMID: 3062176
PDF is available here.
PDF is available here.
Journal of Molecular Biology (203)3 1988
Abstract
We have analyzed the nucleotide sequence changes responsible for mutations from lacIs to lacI- induced in ultraviolet light-irradiated, excision-deficient cells. Irradiated cells were either used as donors in the conjugational transfer of an F' lacIs plasmid to SOS-induced, excision-deficient recipi...
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PMID: 3062175
PDF is available here.
PDF is available here.
Genetika (Moskva) (24)8 1988
Abstract
Plasmids bearing the rifampicin-resistant RNA polymerase beta-subunit gene under control of the lac promoter failed to provide Escherichia coli cells with resistance to rifampicin, despite the accumulation of large quantities of the resistant subunit. The effect proved to be connected with the aggre...
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PMID: 3060397
PDF is available here.
PDF is available here.
Journal of Bacteriology (169)12 1987
Abstract
We examined the junction regions of the cointegrate pPW2 and the corresponding regions of pSK08 (donor) and pRS01 (target) and identified a new IS element on pSK08 (ISS1S) which was involved in and duplicated during formation of pPW2. ISS1S was 808 base pairs (bp) in size, had 18-bp inverted repeats...
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PMID: 2824436
PDF is available here.
PDF is available here.
Nippon Saikingaku Zasshi (42)6 1987
Abstract
Abstract
The 131.1-kilobase (kb) bacteriocin production (Bac) plasmid pNP2 and the 63.6-kb lactose metabolism (Lac) plasmid pCS26, from Streptococcus lactis subsp. diacetylactis WM4, as well as pWN8, a 116.7-kb recombinant plasmid from a Lac+ transconjugant, were analyzed with restriction enzymes to determin...
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PMID: 3038015
PDF is available here.
PDF is available here.
Journal of Bacteriology (159)2 1984
Abstract
A nonconjugative kanamycin-resistant (Kmr) recombinant plasmid, pNR5311, transferred at a low frequency from an Hfr or F' lac Escherichia coli donor to an F- lac- recipient. Among the transconjugants, two types of Kmr plasmids were found: one was indistinguishable from pNR5311 (type A), and the othe...
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PMID: 6086578
PDF is available here.
PDF is available here.
Abstract
Temperature sensitive dnaA recipient crossed at the restrictive temperature with HfrH, free from contaminating F+ cells, forms recombinants almost as proficiently as at the permissive temperature. The merozygotes are able to synthesize DNA at 42 degrees C, although the recipient and donor cells do n...
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PMID: 6205545
PDF is available here.
PDF is available here.
Abstract
The E. coli F'lac plasmid was transferred from an Erwinia chrysanthemi Hfr8 donor to a multiply-auxotrophic, rifampicin-resistant Pseudomonas syringae pv. glycinea recipient. Transfer occurred at a frequency of approximately 10(-5)/donor. Stable transconjugants which were able to utilize lactose as...
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PMID: 6394959
PDF is available here.
PDF is available here.
Abstract
These results demonstrate that the fidelity of conjugal DNA replication is considerably lower than that of vegetative DNA replication....
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PMID: 6357941
PDF is available here.
PDF is available here.
Journal of Bacteriology (154)1 1983
Abstract
We have constructed phoA-lacZ gene fusions. Two of the fusions encode hybrid proteins containing approximately half of alkaline phosphatase at the amino terminus joined to beta-galactosidase. For the one fusion strain analyzed in detail, it was shown that the hybrid protein is found in the membrane...
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PMID: 6403507
PDF is available here.
PDF is available here.
Journal of Bacteriology (154)1 1983
Abstract
The production and regeneration of bacterial protoplasts promoted the loss of three different plasmid-specified traits in Streptococcus lactis subsp. diacetylactis strains. The loss of five different plasmids, including small multicopy molecules, was readily detected in Streptococcus lactis 712 by s...
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PMID: 6403500
PDF is available here.
PDF is available here.
Annales de microbiologie (134B)3 1983
Abstract
DNA analysis showed the presence of plasmids in all 29 strains of mesophilic lactic streptococci. Their number ranged from 1 to 9 and their molecular weight extended from 1.8 to 45 megadaltons (Md). Comparison of the plasmid profiles from 9 strains of Streptococcus lactis, 3 strains of S. lactis ssp...
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PMID: 6326641
PDF is available here.
PDF is available here.
Journal of Bacteriology (146)3 1981
Abstract
Our results suggest that the genes responsible for cell aggregation and high-frequency conjugation are on the segment of deoxyribonucleic acid which recombined with the 33-Mdal lactose plasmid in S. lactis ML3....
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PMID: 6787018
PDF is available here.
PDF is available here.
Abstract
Southern hybridization experiments carried out between pSC101::Tn951 DNA and lambda dlac DNA allowed the location and orientation of the lac operon within the transposon to be deduced. The same method was used to detect Tn951 on Lac plasmids from 11 independent isolates from three continents. None o...
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PMID: 6275007
PDF is available here.
PDF is available here.
Abstract
I alpha. Altogether 14 different groups could be distinguished among R-plasmids on the basis of their phage restriction capability on phage lambda and coliphages T, and another group of R-plasmids showing no restriction at all. Fi+ character was demonstrated in 54.5% of R-plasmids showing phage rest...
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PMID: 7257874
PDF is available here.
PDF is available here.
Biochemistry (Washington) (19)13 1980
Abstract
A small plasmid DNA was assembled into chromatin in vitro by incubation in an extract prepared frog eggs of Xenopus laevis. The plasmid DNA contrained the regulatory region of the Escherichia coli lac operon, the transcription of which is under positive regulation by catabolite activator protein (CA...
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PMID: 6249342
PDF is available here.
PDF is available here.
Abstract
Two plasmids, pLK1 and pLK2, were derived from pIP218, an in vivo recombinant of plasmid F and the drug-resistance plasmid pIP176 (Cmr, Smr, Sur, Tcr). Of these two plasmids, pLK1 is 70 Mdaltons and carries the Tc-resistance determinant in a 7-Mdalton transposition element; pLK2 is 125 Mdaltons and...
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PMID: 6250688
PDF is available here.
PDF is available here.
Annales de microbiologie (131)3 1980
Abstract
These results show that Tn951 is indeed a transposon. A computer program was developed to facilitate mapping of transposon integration sites in plasmids from restriction data....
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PMID: 6252805
PDF is available here.
PDF is available here.
Abstract
Abstract
Abstract
The isolated plasmid DNA of clinical strains of Gram-negative bacteria were shown to have transforming activity when E. coli strain 0600 and S. typhimurium strain LT-2 were used as recipients. The frequency of transformation depended on the recipient strain and the character of the plasmids. The pre...
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PMID: 390935
PDF is available here.
PDF is available here.
Abstract
Large quantities of pure DNA fragments (789, 203 and 95 bp in length) containing the Escherichia coli lac controlling elements (operator, promoter, CRP binding site) were prepared from appropriate recombinant plasmids. High pressure liquid chromatography on RPC-5 or preparative sucrose gradient cent...
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PMID: 387526
PDF is available here.
PDF is available here.
Abstract
Abstract
Journal of Biological Chemistry (254)12 1979
Abstract
I fragment containing the lac promoter and operator was cloned similarly. Also, the 203-base pair fragment was cloned into the Eco RI site of pVH51 using a 300-base pair linker fragment (isolated by RPC-5 column chromatography) which permitted retention of its Hae III ends. Mapping studies on pVH51...
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PMID: 376510
PDF is available here.
PDF is available here.
Abstract
I is not. The functional T 1/2 was increased in pnp mutants and was 95 min during the recovery period. The rate of chemical decay is so slow that the half-life cannot be accurately determined....
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PMID: 386032
PDF is available here.
PDF is available here.
Abstract
Escherichia coli was depleted of ribosomes by a thermal shock at 47 degrees C which quantitatively destroyed the 30S ribosomal subunits. During recovery in minimal medium at 30 degrees C RNA is synthesized while protein synthesis resumes only after about 90 min. It is shown that lac mRNA is synthesi...
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PMID: 386031
PDF is available here.
PDF is available here.
Genetics Research (33)2 1979
Abstract
Journal of Bacteriology (137)2 1979
Abstract
These results suggest that S. lactis strain DR1251 metabolizes galactose primarily via the tagatose-6-phosphate pathway, using a lactose phosphoenolpyruvate phosphotransferase activity to transport this substrate into the cell. Lac(-) derivatives of strain DR1251, deficient in the lactose phosphoeno...
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PMID: 106044
PDF is available here.
PDF is available here.
Abstract
Abstract
Abstract
Abstract
Various molecules generated by transposition of the lactose transposon Tn 951 from plasmid pGC1 to plasmid RP1 were examined by DNA heteroduplex and restriction endonuclease analysis. Tn 951 was found to transpose to at least eight different sites on RP 1 in both possible orientations. A coordinate...
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PMID: 285315
PDF is available here.
PDF is available here.
Abstract
Nine industrially important strains of Streptococcus cremoris (HP, AM(2), ML(1), WC, C(3), R(1), E(8), KH, and Wg(2)) were shown to possess a diversity of plasmid molecules. Molecular weights of plasmids were determined from their relative mobilities after agarose gel electrophoresis and via electro...
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PMID: 736546
PDF is available here.
PDF is available here.
Abstract
Abstract
Abstract
Journal of Biological Chemistry (253)12 1978
Abstract
Biochemical Journal (171)3 1978
Abstract
1. Acridine Orange inhibits growth of Escherichia coli K12 when incubated at pH 7.9, but not at pH 7.4.2. At a non-permissive temperature for DNA polymerase I, Acridine Orange inhibits growth of a temperature-sensitive strain and also increases the rate of elimination of the F'-Lac plasmid. 3. DNA i...
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PMID: 27167
PDF is available here.
PDF is available here.
Abstract
We describe a very sensitive method to detect as antigens the presence of specific proteins within phage plaques or bacterial colonies. We coat plastic sheets with antibody molecules, expose the sheet to lysed bacteria so that a released antigen can bind, and then label the immobilized antigen with...
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PMID: 96441
PDF is available here.
PDF is available here.