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Plant Tumor-Inducing Plasmids (0):
Recent article
Plant Tumor-Inducing Plasmids
Journal of Bacteriology (191)10 2009
Abstract
We demonstrate that two Ti plasmids of Agrobacterium tumefaciens encode robust entry exclusion functions. Two proteins, TrbJ and TrbK, can each mediate entry exclusion and act synergistically. The trbJ and trbK genes are included within the trb operon, which is tightly regulated by the quorum-sensin...
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PMID: 19304847
PDF is available here.
PDF is available here.
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Abstract
The Agrobacterium tumefaciens oncogenic Ti plasmids replicate and segregate to daughter cells via repABC cassettes, in which repA and repB are plasmid partitioning genes and repC encodes the replication initiator protein. repABC cassettes are encountered in a growing number of plasmids and chromosom...
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PMID: 18664372
PDF is available here.
PDF is available here.
Abstract
We report the construction of a family of vectors that contain a reengineered lacI(q)-lac promoter-operator complex in which cloned genes are strongly repressed in the absence of inducer. The vectors, all based on the broad-host-range plasmid pBBR1, are mobilizable and stably replicate at moderate c...
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PMID: 18606801
PDF is available here.
PDF is available here.
Current Microbiology (57)1 2008
Abstract
We constructed the plasmid pZR and transferred it into A. tumefaciens LBA4404 to form the strain LBA4404-ZR. LBA4404-ZR was cocultivated with C. glycerologenesis, and putative transformants were identified by selection for zeocin resistance. Polymerase chain reaction and Southern blot analysis confi...
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PMID: 18415036
PDF is available here.
PDF is available here.
Journal of Bacteriology (190)13 2008
Abstract
We examined the kinetics of induction of conjugative transfer in response to exogenous acyl-homoserine lactone. In the absence of the antiactivator TraM, onset of induction of transfer requires about 30 min, 15 to 20 min of which is needed for expression and construction of the conjugative apparatus...
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PMID: 18203831
PDF is available here.
PDF is available here.
Journal of Biological Chemistry (282)27 2007
Abstract
We showed that TraR and TraM form a 151-kDa stable complex composed of two TraR and two TraM dimers both in vitro and in vivo. When interacted with TraR bound to tra box DNA, wild-type TraM formed a nucleoprotein complex of 77 kDa composed of one dimer of each protein and DNA. The complex converted...
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PMID: 17475619
PDF is available here.
PDF is available here.
Abstract
Agrobacterium tumefaciens KAT23 isolated from peach root causes crown gall disease in a number of grain legume plants, including the common bean (Phaseolus vulgaris) and soybean (Glycine max). KAT23 caused tumor formation in each of these plants more effectively than strain C58. Biotype determinatio...
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PMID: 17617710
PDF is available here.
PDF is available here.
Genes & Genetic Systems (82)3 2007
Abstract
We constructed recombinant plasmids carrying the repABC operon and different portions of pTi-SAKURA. Cells having the recombinant plasmids that harbored a 2.6-kbp NheI fragment of pTi-SAKURA were found to be transformed via conjugation 100-fold less frequently with a small incompatible repABC plasmi...
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PMID: 17660690
PDF is available here.
PDF is available here.
Molecular Microbiology (63)6 2007
Abstract
We had predicted based upon earlier studies. In particular, traA was expressed at surprisingly weak levels. Genetic analysis showed that the traAFBH operon is repressed by formation of a putative relaxosome at oriT consisting the TraA, TraC and TraD. These proteins also repressed the divergent traCD...
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PMID: 17367394
PDF is available here.
PDF is available here.
Plant Cell Reports (25)8 2006
Abstract
A rapid and reproducible Agrobacterium-mediated transformation protocol for sorghum has been developed. The protocol uses the nptII selectable marker gene with either of the aminoglycosides geneticin or paromomycin. A screen of various A. tumefaciens strains revealed that a novel C58 nopaline chromo...
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PMID: 16528567
PDF is available here.
PDF is available here.
Abstract
Migration of agrobacteria inside plants is a complex phenomenon and more extensive than previously reported. Therefore, efficient and sensitive detection methods such as PCR must be used to select clean plants to avoid latent infections of Agrobacterium spp. SIGNIFICANCE AND IMPACT OF THE STUDY: The...
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PMID: 16882149
PDF is available here.
PDF is available here.
Abstract
Hairy roots of maize were induced by infecting 15-d calli with Agrobacterium rhizogenes. The hairy roots cultured in hormone-free media showed the vigorous growth and typical hairy root features. The regenerated plants were produced from hairy roots in MS media supplemented with 1.6 mg/L ZT and 0.4...
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PMID: 16989275
PDF is available here.
PDF is available here.
Abstract
We report characteristic morphological abnormalities of the leaves of transgenic tobacco and Arabidopsis that express 6b from pTiAKE10 (AK-6b), and altered expression of genes related to cell division and meristem formation in the transgenic plants. Cotyledons and leaves of both transgenic tobacco a...
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PMID: 16547081
PDF is available here.
PDF is available here.
Abstract
A binary vector is a standard tool in the transformation of higher plants mediated by Agrobacterium tumefaciens. It is composed of the borders of T-DNA, multiple cloning sites, replication functions for Escherichia coli and A. tumefaciens, selectable marker genes, reporter genes, and other accessory...
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PMID: 16988331
PDF is available here.
PDF is available here.
Abstract
We present dependable methods for the isolation of genomic (total) DNA, mega-plasmid DNA, shuttle or binary plasmid DNA, and RNA. In addition, we provide a simple method for the electronic transfer of shuttle plasmids from Agrobacterium to E. coli for use when their low copy number in Agrobacterium...
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PMID: 16988334
PDF is available here.
PDF is available here.
Abstract
The ability of Agrobacterium to transform plants and other organisms is under highly regulated genetic control. Two Virulence (Vir) proteins, VirA and VirG, function as a two-component regulatory system to sense particular phenolic compounds synthesized by wounded plant tissues. Induction by these p...
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PMID: 16988335
PDF is available here.
PDF is available here.
Abstract
Agrobacterium tumefaciens has been widely used to transform numerous plant species. Frequently, investigators want to place more than one gene into Agrobacterium in order to manipulate various bacterial functions during plant genetic transformation. These genes are frequently brought into the bacter...
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PMID: 16988333
PDF is available here.
PDF is available here.
Current Genetics (48)6 2005
Abstract
We have developed an Agrobacterium tumefaciens-mediated transformation (ATMT) protocol for the plant pathogenic fungus Colletotrichum graminicola, the cause of anthracnose leaf blight and stalk rot of corn. The ATMT results in higher transformation efficiencies than previously available polyethylene...
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PMID: 16292539
PDF is available here.
PDF is available here.
Plant Physiology (139)3 2005
Abstract
The transfer of DNA from Agrobacterium to plant cell nuclei is initiated by a cleavage reaction within the 25-bp right border of Ti plasmids. In an effort to develop all-native DNA transformation vectors, 50 putative right border alternatives were identified in both plant expressed sequence tags and...
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PMID: 16244143
PDF is available here.
PDF is available here.
Molecular Microbiology (58)4 2005
Abstract
We have identified a fourth gene (repD) in the repABC operon of an octopine-type Ti plasmid. repD is 78 codons in length, and maps between repA and repB genes. A repD-lacZ protein fusion demonstrated that repD is strongly expressed. Two identical binding sites for the RepB protein were found within...
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PMID: 16262794
PDF is available here.
PDF is available here.
Abstract
We have used DNA microarrays, representing all genes of the octopine- and nopaline-type Ti plasmids, to identify all Ti-plasmid-encoded genes in the vir regulons of both plasmids. Acetosyringone (AS) induced the expression of all known members of the vir regulons, as well as a small number of additi...
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PMID: 16195384
PDF is available here.
PDF is available here.
Abstract
We show that a CK biosynthesis enzyme, Tmr, which is encoded by the Agrobacterium T-DNA region, is targeted to and functions in plastids of infected plant cells, despite having no typical plastid-targeting sequence. Evidence is provided that Tmr is an adenosine phosphate-isopentenyltransferase (IPT)...
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PMID: 15998742
PDF is available here.
PDF is available here.
Molecular Microbiology (56)6 2005
Abstract
We have identified a new gene (repE) within the repB-repC intergenic region of an octopine-type Ti plasmid. This gene encodes a small, non-translated RNA that is transcribed in the direction opposite to the repABC mRNA. Increased expression of repE blocked plasmid replication of a repABC-dependent m...
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PMID: 15916607
PDF is available here.
PDF is available here.
Abstract
The semi-nested PCR-based method allowed for sensitive and rapid detection of tumorigenic agrobacteria in soil. SIGNIFICANCE AND IMPACT OF THE STUDY: The method is proposed for testing of soil in fields intended for nursery production of fruit trees, roses or other plants susceptible to crown gall,...
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PMID: 15715875
PDF is available here.
PDF is available here.
Microbiological Research (160)1 2005
Abstract
We cloned the virE2 gene into plasmid pQE31 in Escherichia coli cells. VirE2 protein was isolated from E. coli XL-1 blue cells containing a recombinant plasmid, pQE31-virE2. The cells were ultrasonically disrupted, and the protein containing six histidine residues at the N-terminal end was isolated...
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PMID: 15782940
PDF is available here.
PDF is available here.
Molecular Microbiology (53)5 2004
Abstract
TraM, an 11.2 kDa antiactivator, modulates the acyl-homoserine lactone-mediated autoinduction of Ti plasmid conjugative transfer by interacting directly with TraR, the quorum-sensing transcriptional activator. Most antiactivators and antisigma factors examined to date act in dimer form. However, whe...
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PMID: 15387823
PDF is available here.
PDF is available here.
Abstract
We investigated the expression pattern of the promoter of Nicotiana glauca (Ng) ORF13 in the hybrids between N. glauca and N. langsdorffii harboring the NgORF13-beta-glucuronidase (GUS) chimeric gene. The promoter of NgORF13 of N. glauca had lower activities than the promoter of RiORF13 of Agrobacte...
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PMID: 15356328
PDF is available here.
PDF is available here.
Microbial Ecology (48)1 2004
Abstract
A conjugal donor system, ST2, was constructed to study the conjugal dissemination of a Ti plasmid to wild-type recipient bacteria in vitro and in situ. The system consisted of a polyauxotrophic derivative of C58 harboring a hyperconjugative and highly selectable Ti plasmid, pSTiEGK, which was constr...
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PMID: 15164241
PDF is available here.
PDF is available here.
Molecular Microbiology (52)6 2004
Abstract
The quorum-sensing transcriptional activator TraR of Agrobacterium tumefaciens, which controls the replication and conjugal transfer of the tumour-inducing (Ti) virulence plasmid, is inhibited by the TraM antiactivator. The crystal structure of TraM reveals this protein to form a homodimer in which...
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PMID: 15186414
PDF is available here.
PDF is available here.
Abstract
The Agrobacterium-mediated transformation of Aspergillus awamori was optimized using defined co-cultivation conditions, which resulted in a reproducible and efficient transformation system. Optimal co-cultivation conditions were used to study the role of Agrobacterium tumefaciens virulence proteins...
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PMID: 15050546
PDF is available here.
PDF is available here.
Plant Physiology (133)3 2003
Abstract
We show here that the absence of pAtC58 in pTiC58-containing strains results in reduced virulence but that disruption of the attR gene does not result in avirulence or a reduction in virulence. Our studies indicate that pAtC58 has a positive effect on vir gene induction as revealed by immunoblot ana...
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PMID: 14551325
PDF is available here.
PDF is available here.
Abstract
The nucleotide sequence of the biphenyl catabolic transposon Tn4371 has been completed and analyzed. It confirmed that the element has a mosaic structure made of several building blocks. In addition to previously identified genes coding for a tyrosine recombinase related to phage integrases and for...
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PMID: 12902278
PDF is available here.
PDF is available here.
Abstract
The Agrobacterium tumefaciens C58 genome contains three putative N-acyl homoserine lactone (acyl-HSL) hydrolases, which are closely related to the lactonase AiiA of Bacillus. When expressed in Escherichia coli, two of the putative acyl-HSL hydrolases, AttM and AiiB, conferred the ability to degrade...
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PMID: 12902298
PDF is available here.
PDF is available here.
Molecular Microbiology (49)2 2003
Abstract
We describe a fourth promoter, designated P4. This promoter lies directly upstream of repA and is not regulated by TraR. The promoter was localized by subcloning and demonstrated to be strongly autorepressed. RepA is the major cis-acting autorepressor of this promoter, though RepB enhanced repressio...
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PMID: 12828641
PDF is available here.
PDF is available here.
Abstract
The chromosomal ros gene in Agrobacterium tumefaciens encodes a repressor of virulence and oncogenes that are located on a resident Ti plasmid. Mutational inactivation of ros de-represses the expression of the virC and virD operons, causing premature processing and accumulation of T-DNA molecules, a...
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PMID: 12460533
PDF is available here.
PDF is available here.
Genes & Genetic Systems (77)1 2002
Abstract
We developed a novel curing method based on plasmid incompatibility. For this, a curing plasmid, pMGTrep1, was newly constructed and subsequently introduced into A. tumefaciens strains harboring pTi by conjugation with Escherichia coli harboring pMGTrep1. The conjugation yielded 32-99% nopaline non-...
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PMID: 12036099
PDF is available here.
PDF is available here.
EMBO Journal (5)6 1986
Abstract
We developed a binary vector system. One plasmid (= substrate) contains correctly oriented right and left borders from octopine plasmid pTiAch5. A gene with a counterselectable function (galK) was cloned between these borders. The galK gene is under control of the tac promoter-operator and the lac r...
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PMID: 15966100
PDF is available here.
PDF is available here.
EMBO Journal (5)6 1986
Abstract
We tested the activity of a border repeat surrounded by different amounts of normal flanking sequences. Efficient T-DNA transmission required a conserved sequence (5' TAAPuTPy-CTGTPuT-TGTTTGTTTG 3') which lies to the right of the two known right border repeats. In either orientation, a synthetic oli...
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PMID: 15966101
PDF is available here.
PDF is available here.
EMBO Journal (4)4 1985
Abstract
We investigated whether it codes for an enzyme of hormone biosynthesis. In a first set of experiments, gene 4 from octopine plasmid pTiAch5 and nopaline plasmid pTiC58 was expressed in Escherichia coli, and the gene products were identified by reaction with antiserum raised against a decapeptide der...
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PMID: 15938044
PDF is available here.
PDF is available here.