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Welcome to the Pubget Medical Subject Headings (MESH) browser. Click on a topic or subtopic below to explore related papers. In the gold box is the most recent paper about the current MESH term, and below are 20 related papers.
Plasmids (7):
Recent article
Plasmids
Abstract
We demonstrate that a single, site-specific, cyclobutane pyrimidine dimer leading-strand template lesion provides only a transient block to fork progression in vitro. The replisome remains stably associated with the fork after collision with the lesion. Leading-strand synthesis is then reinitiated d...
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PMID: 21998391
PDF is available here.
PDF is available here.
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Biomacromolecules (12)9 2011
Abstract
Polyhydroxyalkanoates (PHA) synthesis genes phbC and orfZ cloned from Ralstonia eutropha H16 were transformed into beta-oxidation weakened Pseudomonas putida KTOY08ΔGC, a mutant of P. putida KT2442. The recombinant P. putida strain termed KTHH06 was able to produce a short-chain-len...
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PMID: 21863836
PDF is available here.
PDF is available here.
Abstract
We demonstrate T4SS-mediated DNA transfer from a human bacterial pathogen into human cells. We show that the zoonotic pathogen Bartonella henselae can transfer a cryptic plasmid occurring in the bartonellae into the human endothelial cell line EA.hy926 via its T4SS VirB/VirD4. DNA transfer into EA.h...
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PMID: 21844337
PDF is available here.
PDF is available here.
Abstract
We have carried out a series of molecular manipulations targeting GD3 acetylation pathways. Using immunocytochemistry, flow cytometry, western blotting, and transwell assays, we have shown the existence of a critical ratio between GD3 and 9-O-acetyl GD3, which promotes tumor survival. Thus, we have...
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PMID: 21807667
PDF is available here.
PDF is available here.
Abstract
Polyethylenimine-cyclodextrin-tegafur (PEI-CyD-tegafur) conjugate was synthesized as a novel multifunctional prodrug of tegafur for co-delivery of chemotherapeutic agent tegafur and enhanced green fluorescent protein (EGFP) reporter plasmid DNA. Conjugation of tegafur to PEI-CyD via...
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PMID: 21887847
PDF is available here.
PDF is available here.
Abstract
Expressing proteins of interest as fusion to proteins of bacterial envelope is a powerful technique for biotechnological and medical applications. The synthetic gene (VacII) encoding for T-cell epitopes of selected genes of Mycobacterium tuberculosis namely, ESAT6, MTP40, 38 kDa, and...
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PMID: 21941936
PDF is available here.
PDF is available here.
Abstract
Parvoviruses are small, 260-A-diameter, icosahedral, non-enveloped, single-stranded DNA viruses with a genome of approximately 5 kb. Non structural protein, (NS-1) is especially relevant, being both essential for virus replication and the main factor responsible for virus pathogenici...
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PMID: 21941937
PDF is available here.
PDF is available here.
Abstract
The present study was undertaken to clone, express rabies virus glycoprotein (RVG) and to identify potential T-cell epitopes on it. RVG gene (1590 bp) was amplified using gene specific primers. The amplified product was cloned into pTZ57R/T cloning vector by TA cloning. RVG gene was...
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PMID: 21870427
PDF is available here.
PDF is available here.
Biomacromolecules (12)6 2011
Abstract
We have designed and produced a modular, recombinant resilin-like polypeptide bearing both mechanically active and biologically active domains to create novel biomaterial microenvironments for engineering mechanically active tissues such as blood vessels, cardiovascular tissues, and vocal folds. Pre...
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PMID: 21553895
PDF is available here.
PDF is available here.
Protein Journal (30)5 2011
Abstract
We utilized a proteomics strategy to characterize proteome-wide alterations between MP-treated MKN28 lines and their untreated counterparts. A total of 97 spots were positively identified as differentially expressed, and of these 62 proteins were up-regulated, whereas 35 proteins were down-regulated...
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PMID: 21574062
PDF is available here.
PDF is available here.
Abstract
Abstract
We propose a model whereby an N-terminal signal sequence, xLxxKN, directs ABCA transporters to a post-Golgi vesicular sorting station where additional signals may be required for selective delivery of individual transporters to final subcellular destinations....
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PMID: 21586796
PDF is available here.
PDF is available here.
Audio, Transactions of the IRE Professional Group on (Chapter 14) 2011
Abstract
Invitrogen's Gateway technology is a recombination-based cloning method that allows for rapid transfer of numerous open reading frames (ORFs) into multiple plasmid vectors, making it useful for diverse high-throughput applications. Gateway technology has been utilized to create an OR...
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PMID: 21538302
PDF is available here.
PDF is available here.
Cold Spring Harbor Protocols (2011)5 2011
Abstract
Engineering effective vectors has been crucial to the efficient delivery and expression of therapeutic gene products in vivo. Among these, HSV-1 represents an excellent candidate vector for delivery to the peripheral and central nervous systems. The natural biology of HSV-1 includes the establishmen...
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PMID: 21536761
PDF is available here.
PDF is available here.
Xenobiotica (41)5 2011
Abstract
Studies described in the recent literature support the idea that gene therapy can lead to genuine clinical benefits when mediated by plasmid delivery in conjunction with electroporation. Plasmid-mediated muscle-targeted gene transfer offers the potential of a cost-effective pharmaceutical-grade ther...
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PMID: 18370221
PDF is available here.
PDF is available here.
Xenobiotica (41)5 2011
Abstract
We have optimized the electroporation conditions in a large mammal model, i.e. pig. The parameters optimized include electric field intensity, number of pulses, lag time between plasmid injection and electroporation, and plasmid delivery volume. Constant current pulses, between 0.4 and 0.6 A, applie...
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PMID: 18370201
PDF is available here.
PDF is available here.
Xenobiotica (41)5 2011
Abstract
Xenobiotica (41)5 2011
Abstract
The discovery of novel target antigens for antibody-based immunotherapy is still a major challenge. Antibody phage display is one of the technologies that is widely applied for the identification of novel cell surface molecules on intact eukaryotic cells and many reports describe the isolation of ph...
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PMID: 19153691
PDF is available here.
PDF is available here.
Xenobiotica (41)5 2011
Abstract
We describe a recombinant method for the construction and production of a novel IgG-like BsAb molecule, using the variable domains of two fully human antibodies as the building blocks....
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PMID: 19252849
PDF is available here.
PDF is available here.
Xenobiotica (41)5 2011
Abstract
We discuss that the DNA delivery into skin can be greatly enhanced by topical electroporation of the DNA injection site in rabbits using a tweezer electrode. Furthermore, the immune responses following a DNA vaccine delivery by using electroporation have been explored. Electroporation shows great po...
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PMID: 18370202
PDF is available here.
PDF is available here.
Xenobiotica (41)5 2011
Abstract
Using gene therapy to produce systemic levels of human factor IX for the treatment of hemophilia B has been clinically evaluated using viral-based vectors. The efficacy of this approach has been limited because of immune responses against the viral components. An alternative approach is to use physi...
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PMID: 18370215
PDF is available here.
PDF is available here.
Xenobiotica (41)5 2011
Abstract
Microbodies are novel pharmacophoric entities which are derived from naturally occurring cystine-knot microproteins. They provide extremely stable scaffolds that can be engineered to high-affinity binding proteins. A peptide-grafting approach yielded specific ligands for human thrombopoietin recepto...
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PMID: 19377985
PDF is available here.
PDF is available here.
Abstract
To investigate the relationship between the expression of thymidine phosphorylase (TP) and the sensitivity of gastric carcinoma to 5-fluorouracil (5-FU) and its prodrugs.
Gastric carcinoma cell line AGS was transfected with recombinant plasmid pEGFP-N1-TP or control p...
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PMID: 21756828
PDF is available here.
PDF is available here.
Abstract
Abstract
Gene delivery into liver cancer cells has been a problem. This study aimed to understand the effect of using PEGI/Fe(3)O(4) nanomagnetic fluid as a gene vector for liver cancer gene therapy. An AFP enhancer aids in the expression of tumor-specific foreign genes in AFP-producing cancer cells like HepG...
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PMID: 21621154
PDF is available here.
PDF is available here.
Bing du xue bao = Chinese journal of virology / [bian ji, Bing du xue bao bian ji wei yuan hui] (27)3 2011
Abstract
Our results provide a basis for further exploring diagnostic role and vaccine development of different spike segments from HCoV-NL63....
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PMID: 21774251
PDF is available here.
PDF is available here.
Bing du xue bao = Chinese journal of virology / [bian ji, Bing du xue bao bian ji wei yuan hui] (27)3 2011
Abstract
Abstract
Detection of osteogenic differentiation is crucial for bone tissue engineering. Despite established standard end point assays, there is increasing demand for methods allowing noninvasive kinetic differentiation monitoring. Reporter gene assays employing tissue-specific promoters and...
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PMID: 21043997
PDF is available here.
PDF is available here.
Xi bao yu fen zi mian yi xue za zhi = Chinese journal of cellular and molecular immunology (27)4 2011
Abstract
To prokaryotically express and purify fusion protein containing extracellular region of human BTLA and prepare the antiserum of it.
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PMID: 21481321
PDF is available here.
PDF is available here.
Hereditas (Beijing) (33)4 2011
Abstract
Our previous studies showed that tandem Alu repeats inhibited GFP gene expression when they were inserted into the downstream of GFP gene in pEGFP-C1 vector and HeLa cells were then transfected transiently. The sequence named 2F2R (second 60 bp from the 5' end of SV40PolyA antisense strand) eliminate...
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PMID: 21482523
PDF is available here.
PDF is available here.
Abstract
RNA interference (RNAi) mediated by short hairpin-RNA (shRNA) expressing plasmids can induce specific and long-term knockdown of specific mRNAs in eukaryotic cells. To develop a vector-based RNAi model for Schistosoma mansoni, the schistosome U6 gene promoter was employed to drive expression of shRNA...
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PMID: 21447344
PDF is available here.
PDF is available here.
Abstract
In order to observe the dosage-effect of recombinant pig interleukin-6 gene and CpG motifs on the immune responses of swine to vaccine, a novel recombinant eukaryotic VPIL6C plasmid was packed with chitosan nanoparticles (CNP) prepared by ionic cross linkage, which contains pig interleukin-6 gene and...
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PMID: 21443961
PDF is available here.
PDF is available here.
Abstract
We investigate whether endothelial lipase (EL) also requires Lmf1 to attain enzymatic activity. We demonstrate that cells harboring a (cld) loss-of-function mutation in the Lmf1 gene are unable to generate active EL, but they regain this capacity after reconstitution with the Lmf1 wild type. Further...
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PMID: 21447484
PDF is available here.
PDF is available here.
Abstract
Abstract
We tested 753 gram-negative bloodstream isolates for AmpC by using the EDTA disk test and the modified Hodge test (n = 172) and the modified Hodge test alone (n = 581). The 30-day mortality for the AmpC group was 9% (2/23) and was 6% (3/51) for the control group. The clinical response was similar: a...
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PMID: 21411779
PDF is available here.
PDF is available here.
Abstract
We report generation of transgenic (Tg) mice with targeted Elovl4 expression driven by an epidermal-specific involucrin promoter. In homozygous Stgd3 mice, this transgene reinstates both epidermal Elovl4 expression and synthesis of two missing epidermal lipid groups: C28-C36 acylceramides and (O-lin...
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PMID: 21429867
PDF is available here.
PDF is available here.
Current Biology (21)5 2011
Abstract
We show that a conserved motif in the N-terminal region of Cenp-C binds directly and with high affinity to the Mis12 complex. Expression in HeLa cells of the isolated N-terminal motif of Cenp-C prevents outer kinetochore assembly, causing chromosome missegregation. The KMN network is also responsibl...
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PMID: 21353556
PDF is available here.
PDF is available here.
Abstract
Food poisoning and non-food poisoning illnesses due to C. perfringens (by enterotoxin production) have been associated to chromosomal or plasmidic location of the cpe gene, respectively. Clostridial pathogenicity has been correlated to protease and azoreductase production. The aim of this work was: i...
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PMID: 21526657
PDF is available here.
PDF is available here.
Abstract
We identify a previously uncharacterized type of bacterial communication mediated by nanotubes that bridge neighboring cells. Using Bacillus subtilis as a model organism, we visualized transfer of cytoplasmic fluorescent molecules between adjacent cells. Additionally, by coculturing strains harborin...
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PMID: 21335240
PDF is available here.
PDF is available here.
Abstract
A family of biodegradable poly(amine-co-esters) was synthesized in one step via enzymatic copolymerization of diesters with amino-substituted diols. Diesters of length C(4) -C(12) (i.e., from succinate to dodecanedioate) were successfully copolymerized with diethanolamines with eithe...
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PMID: 21171165
PDF is available here.
PDF is available here.
Nature Protocols (6)2 2011
Abstract
We provide a protocol for a sequence-independent approach for cloning complex individual or combinatorial DNA libraries, and routine or high-throughput cloning of single or multiple DNA fragments. The strategy, called circular polymerase extension cloning (CPEC), is based on polymerase overlap exten...
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PMID: 21293463
PDF is available here.
PDF is available here.
Tsitologiia (53)5 2011
Abstract
Effect of carcinogenic polycyclic aromatic hydrocarbons (PAH) benzo(a)pyrene (BP) and 3-methylcholanthrene (MC) on transcription factor NF-kappaB activation was studied. The determination of NF-kappaB activity was performed by two different methods: determination of mRNA expression of NF-kappaB-depen...
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PMID: 21786685
PDF is available here.
PDF is available here.
Abstract
We demonstrate in vivo movement of a restriction-modification system within a genome under a laboratory condition. After blocking replication of a temperature-sensitive plasmid carrying a PaeR7I restriction-modification system in Escherichia coli cells, the plasmid was found integrated into the chro...
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PMID: 21305031
PDF is available here.
PDF is available here.
Abstract
We have shown previously that spontaneous lateral transfer of non-conjugative plasmids occurs in a colony biofilm of mixed E. coli strains (a set of a donor strain harbouring a plasmid and a plasmid-free recipient strain). In this study, with high-frequency combinations of strains and a plasmid, we...
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PMID: 21283723
PDF is available here.
PDF is available here.
Abstract
We introduce a biosynthetic system that permits studies of both channel activity in single cells and electrical conduction in multicellular networks. We convert unexcitable somatic cells into an autonomous source of electrically excitable and conducting cells by stably expressing only three membrane...
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PMID: 21556054
PDF is available here.
PDF is available here.
Abstract
These results demonstrated that AOBs system is an efficient method to functionally express and purify small AMPs. In addition, it also provides a facile platform for the production of antibodies against small peptides. © 2010 American Institute of Chemical Engineers Biotechnol. Prog., 2011....
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PMID: 21312363
PDF is available here.
PDF is available here.
Abstract
We show that the CatD ortholog of the budding yeast Saccharomyces cerevisiae (Pep4p) harbors a dual cytoprotective function, composed of an anti-apoptotic part, conferred by its proteolytic capacity, and an anti-necrotic part, which resides in the protein's proteolytically inactive propeptide. Thus,...
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PMID: 21593793
PDF is available here.
PDF is available here.
Biology Direct (6)1 2011
Abstract
We examined the specificity of several commercially available anti-phosphorylated GSK-3 antibodies. We show that antibodies raised to peptides containing the phosphorylated Ser21/9 epitope crossreact with unidentified antigens that are highly expressed by mitotic cells and that mainly localise to sp...
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PMID: 21261990
PDF is available here.
PDF is available here.
Abstract
We provide evidence that the C-terminal UBA domains guard ubiquitin receptors from destruction by preventing initiation of degradation at the proteasome. We show that introduction of unstructured polypeptides that are sufficiently long to function as initiation sites for degradation abrogates the pr...
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PMID: 21304520
PDF is available here.
PDF is available here.
Abstract
We provide a detailed protocol for the use of a high-throughput yeast transformation protocol with a liquid handling robot to perform a plasmid overexpression screen, using an arrayed library of 5,500 yeast plasmids. We have been using these screens to identify genetic modifiers of toxicity associat...
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PMID: 21841759
PDF is available here.
PDF is available here.
Abstract
We provide a detailed protocol of the design of a mammalian cell-to-cell signaling device and its integration into a mammalian quorum-sensing system for cell density-induced expression product genes. Cell-to-cell signaling is based on a sender cell, metabolically engineered for expression of alcohol...
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PMID: 21031316
PDF is available here.
PDF is available here.
Abstract
We have exploited the natural transformability and powerful homologous recombination capacity of this organism by using linear DNA fragments for transformation. This approach overcomes barriers that have made the introduction and expression of foreign genes problematic in the past. Foremost among th...
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PMID: 20960136
PDF is available here.
PDF is available here.
Abstract
A series of protocols are presented for the storage, growth, transformation, and characterization of wild type and mutant strains of Synechocystis sp. strain PCC 6803. These protocols include the isolation of genomic DNA and the strategies required for the construction of specific gene interruptions...
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PMID: 20960137
PDF is available here.
PDF is available here.
Abstract
We have used RNA interference to selectively suppress the expression of aggrecanase genes in human chondrocytes, in an attempt to determine which of these key enzymes have roles in arthritic cartilage destruction. This combination of gene targeting and tissue engineering we are using should be equal...
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PMID: 21042968
PDF is available here.
PDF is available here.
Abstract
We have shown that hESCs can be stably transfected with a plasmid in which expression of the green fluorescent protein (GFP) is under the control of the Oct-4 promoter using chemical transfection. The expression pattern of transgenic Oct-4-GFP reflects that of endogenous Oct-4....
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PMID: 21042987
PDF is available here.
PDF is available here.
American Surgeon (11) 2011
Abstract
Abstract
Studies of influence of Yersinia pestis fra-operon carriage on morphological properties of Y. pseudotuberculosis recipient strain cells and colonies at different temperature and cultivation time.
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PMID: 21598620
PDF is available here.
PDF is available here.
Abstract
We expressed, purified, and characterized the recombinant protein containing immunomimetic peptide of benzo[a]pyrene....
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PMID: 21789801
PDF is available here.
PDF is available here.
Abstract
Effect of constitutive expression of the aceEF-lpdA operon genes coding for the enzymes of NAD+ reducing pyruvate dehydrogenase complex on the anaerobic production of succinic acids from glucose by recombinant Escherichia coli strains was studied. Basic producer strains were obtained by inactivation...
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PMID: 21950115
PDF is available here.
PDF is available here.
Abstract
Bacillus licheniformis WBL-3, one of poly-gamma-glutamic acid (gamma-PGA) producers, depends on the existence of glutamate in the medium. In this paper, gamma-PGA synthetase complex gene (pgsBCA) was cloned from Bacillus licheniformis WBL-3. pgsBCA gene of B. licheniformis WBL-3 was highly homologous...
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PMID: 21950116
PDF is available here.
PDF is available here.