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Welcome to the Pubget Medical Subject Headings (MESH) browser. Click on a topic or subtopic below to explore related papers. In the gold box is the most recent paper about the current MESH term, and below are 20 related papers.
Plasmids (7):
Recent article
Plasmids
Abstract
We have shown previously that immunoglobulin fragment-fused GLP-1 (GLP-1/Fc) is a structurally stable GLP-1 analog. Here, we report a non-viral GLP-1/Fc gene therapy strategy utilizing a REP78-in-trans and REB-in-cis element system to achieve a site-specific genomic integration. For this purpose, th...
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PMID: 21070745
PDF is available here.
PDF is available here.
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Abstract
We show that expression of a dominant-negative subunit of the v-ATPase in neural precursors of the developing mouse cortex depleted neural stem cells by promoting their differentiation and the generation of neurons. Moreover, inhibition of the v-ATPase reduced endogenous Notch signalling and prevent...
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PMID: 21126173
PDF is available here.
PDF is available here.
Abstract
These results indicate that GPR40 mediates the short-term effects of FFAs on GSIS, but does not mediate the chronic lipotoxicity on β-cells. The reverse role of pioglitazone on lipotoxicity of β-cells may be related to GPR40.
Copyright © 2010 Elsevier Inc. All rights reserved....
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PMID: 21036144
PDF is available here.
PDF is available here.
Macromolecular Bioscience (10)11 2010
Abstract
I collagen (COL1A1) transcription, but is functionally limited as a free molecule. Using a matrix-binding domain (MBD) from microfibril-associated glycoprotein-1, the fusion protein MBD-CTGF was targeted to the ECM and tested for COL1A1 transcriptional activation. MBD-CTGF produced by the ECM-synthe...
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PMID: 20799254
PDF is available here.
PDF is available here.
Abstract
We show that the Streptococcus thermophilus CRISPR1/Cas system can also naturally acquire spacers from a self-replicating plasmid containing an antibiotic-resistance gene, leading to plasmid loss. Acquired spacers that match antibiotic-resistance genes provide a novel means to naturally select bacte...
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PMID: 21048762
PDF is available here.
PDF is available here.
Cancer Research (70)21 2010
Abstract
We have examined how HSP90 inhibitors affect LATS1 and the related protein LATS2, two kinases that relay antiproliferative signals in the Hippo tumor suppressor pathway. Both LATS1 and LATS2 were depleted from cells treated with the HSP90 inhibitors 17-allylamino-17-demethoxygeldanamycin (17-AAG), r...
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PMID: 20841485
PDF is available here.
PDF is available here.
Abstract
We also conjugated pSTV into S. Typhi and S. Paratyphi A serovars and evaluated these transconjugants in newborn and infant mice.
We determined that the spv virulence genes from the S. Typhimurium virulence plasmid are expressed in S. Typhi and S. Paratyphi A in a RpoS dependent f...
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PMID: 21252450
PDF is available here.
PDF is available here.
Cancer Science (101)11 2010
Abstract
Dendritic cells (DC) are critical for priming adaptive immune responses to foreign antigens. However, the feasibility of harnessing these cells in vivo to optimize the antitumor effects has not been fully explored. The authors investigated a novel therapeutic approach that involves delivering synerg...
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PMID: 20804501
PDF is available here.
PDF is available here.
Plant Cell Reports (29)11 2010
Abstract
We report an efficient method to produce dsRNA using a bacterial expression system. Two fragments of the Sugarcane Mosaic Virus (SCMV) CP (coat protein) gene were amplified by RT-PCR, and cloned into the inverted-repeat cloning vector pUCCRNAi. The two recombinant plasmids were transformed individua...
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PMID: 20734050
PDF is available here.
PDF is available here.
Zhongguo shi yan xue ye xue za zhi / Zhongguo bing li sheng li xue hui = Journal of experimental hematology / Chinese Association of Pathophysiology (18)6 2010
Abstract
In order to analyze the possible epigenetic regulation mechanism of mll-af4 gene expression and find the possible microRNA regulating mll-af4 gene expression, targetscan software was used to analyze potential microRNA target sites in 3'-UTR of mll-af4. 3'-UTR fragment of mll-af4 was amplified by PCR....
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PMID: 21176377
PDF is available here.
PDF is available here.
Zhongguo shi yan xue ye xue za zhi / Zhongguo bing li sheng li xue hui = Journal of experimental hematology / Chinese Association of Pathophysiology (18)6 2010
Abstract
In order to explore the effects of 35C > T and 682A > G mutations on the activity of alpha-(1,2) fucosyltransferase, the coding region of fut1 gene was amplified by polymerase chain reaction (PCR) from genomic DNA. PCR product was ligated into expression vector using TOPO TA cloning kit to obtain the...
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PMID: 21176381
PDF is available here.
PDF is available here.
Abstract
We developed novel promoter-probe and terminator-probe vectors based on the Streptococcus agalactiae pMV158 plasmid, which replicates autonomously in numerous Gram-positive bacteria. As reporter gene, a gfp allele encoding a variant of the green fluorescent protein was used. These genetic tools were...
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PMID: 20801171
PDF is available here.
PDF is available here.
Abstract
We report the characterization of a TLC-related element that corresponds to the genome of a satellite filamentous phage (TLC-Knφ1), which uses the morphogenesis genes of another filamentous phage (fs2φ) to form infectious TLC-Knφ1 phage particles. The TLC-Knφ1 phage genome carries a sequence sim...
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PMID: 20944629
PDF is available here.
PDF is available here.
Abstract
We report the development of a suite of six integrative vectors for construction of single copy transcriptional fusions with the gfpmut3, cfp and iyfp reporter genes in Bacillus subtilis. The promoter fusions are constructed using the highly efficient ligation-independent cloning (LIC) technique mak...
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PMID: 20600285
PDF is available here.
PDF is available here.
Abstract
The objective of the present study was to construct and assess the immunogenicity of recombinant adenovirus vectors expressing the HMW1, HMW2, or Hia protein of nontypeable Haemophilus influenzae (NTHi). These proteins are critical adhesins and potential protective antigens expressed...
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PMID: 20685934
PDF is available here.
PDF is available here.
Virologica Sinica (25)5 2010
Abstract
In order to establish the eukaryotic cell lines for inducible control of SARS-CoV nucleocapsid gene expression. The recombinant plasmid of pTRE-Tight-SARS-N was constructed by using the plasmid p8S as the PCR template which contains a cDNA clone covering the nucleocapsid gene of SARS...
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PMID: 20960182
PDF is available here.
PDF is available here.
Abstract
We describe the use of human SUMO1 and SUMO2 as a useful gene fusion technology. Human SUMO1 and SUMO2 fusion expression vectors were constructed and tested in His-tag and ubiquitin fusion expression systems. Two difficult-to-express model proteins, matrix metalloprotease-13 (MMP13) and enhanced gre...
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PMID: 20457256
PDF is available here.
PDF is available here.
Abstract
This review aims to identify and highlight important data published in the past 12 months which provide new information on nontyphoidal salmonellosis in Africa.
Recent reviews and clinical studies continue to emphasize the challenges of diagnosis and management of invasive nontyphoidal Salmonellae (...
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PMID: 20736739
PDF is available here.
PDF is available here.
Abstract
We evaluated the substitution of murine constant regions for their human counterpart within the DMF5 MART-1-specific TCR. Unlike previous studies, all modified TCRs were inserted into retroviral vectors and analyzed for expression and function following a clinical transduction protocol. PBL were tra...
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PMID: 20628878
PDF is available here.
PDF is available here.
Abstract
We observed that Labrasol has the ability to solubilize various cationic Eudragits such as Eudragit RSPO, Eudragit RLPO and Eudragit EPO at a concentration as high as 200 mg/ml. We also evaluated the ability of Labrasol to act as a nanoparticle stabilizer due to its amphiphilic nature and high HLB o...
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PMID: 21105574
PDF is available here.
PDF is available here.
YAKUGAKU ZASSHI (130)10 2010
Abstract
I collect the information of these naked plasmid DNA transfection methods involving tissue pressure-mediated transfection from the comprehensive view point including side effects. Additively, the key physiological phenomena affecting transgene expression, especially activation of transcriptional fac...
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PMID: 20930483
PDF is available here.
PDF is available here.
Abstract
Four novel Zn(II) complexes [Zn(L(1))(bipy)(H(2)O)(2)].4H(2)O(1), [Zn(L(1))(phen)(H(2)O)(2)].4H(2)O(2), [Zn(L(2))(bipy)(H(2)O)(2)].4H(2)O(3) and [Zn(L(2))(phen)(H(2)O)(2)].4H(2)O (4), where bipy=2,2'-bipyridine, phen=1,10-phenanthroline, L(1)=2,2'-bipyridine 5,5'-dicarboxylic acid, L(2)=2,2'-bipyrid...
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PMID: 20692739
PDF is available here.
PDF is available here.
Food and Chemical Toxicology (48)10 2010
Abstract
The antioxidant and pro-oxidant potential of an extract from red radish, in which the major compounds were acylated pelargonidin derivatives, were assessed with a variety of assays in vitro. The extract appeared to form a complex with Fe(3+) or Cu(2+). It displayed a concentration-dependant reducing...
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PMID: 20600520
PDF is available here.
PDF is available here.
Abstract
We performed purification before renaturation in the prokaryotic expression system instead of using the traditional renaturation-before-purification approach. rhBMP-2 was separated on a Sephacryl S-300 HR column and eluted from a DEAE-Sepharose Fast Flow column. The collected protein was refolded by...
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PMID: 19823948
PDF is available here.
PDF is available here.