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Welcome to the Pubget Medical Subject Headings (MESH) browser. Click on a topic or subtopic below to explore related papers. In the gold box is the most recent paper about the current MESH term, and below are 20 related papers.
Templates, Genetic (0):
Recent article
Templates, Genetic
Molecular Cell (32)3 2008
Abstract
We present cryoelectron microscopy reconstructions of bacterial RNAP in complex with sigma(54) alone, and of RNAP-sigma(54) with an AAA+ activator. Together with photo-crosslinking data that establish the location of promoter DNA within the complexes, we explain why the RNAP-sigma(54) closed complex...
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PMID: 18995832
PDF is available here.
PDF is available here.
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Molecular Microbiology (70)3 2008
Abstract
We demonstrated that endogenous AP sites persist at a slightly higher level in P. abyssi genome compared with Escherichia coli. Then, the two replicative DNA polymerases, PabpolB and PabpolD, were characterized in presence of DNA containing abasic sites. Both Pabpols had abortive DNA synthesis upon...
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PMID: 18826407
PDF is available here.
PDF is available here.
BioTechniques (45)5 2008
Abstract
Combinatorial libraries coding for mosaic enzymes with predefined crossover points constitute useful tools to address and model structure-function relationships and for functional optimization of enzymes based on multivariate statistics. The presented method, called sequence-independent generation o...
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PMID: 19007337
PDF is available here.
PDF is available here.
Biochemistry (New York) (73)11 2008
Abstract
We investigate the TLS process catalyzed by DNA polymerases beta and lambda on DNA substrates using mono- or dinucleotide gaps opposite damage located in the template strand. An analog of a natural apurinic/apyrimidinic site, the 3-hydroxy-2-hydroxymetylthetrahydrofuran residue (THF), was used as da...
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PMID: 19120024
PDF is available here.
PDF is available here.
Abstract
We derive a simple expression for the maximum number of distinct templates n(m) that can coexist in a hypercycle and show that it is a decreasing function of the length L of the templates. In the case of high replication accuracy we find that the product n(m)L tends to a constant value, limiting thu...
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PMID: 18694761
PDF is available here.
PDF is available here.
Current protocols in molecular biology / edited by Frederick M. Ausubel ... [et al.] (Chapter 3) 2008
Abstract
Terminal deoxynucleotidyl transferase (TdT), is a template-independent DNA polymerase that catalyzes the incorporation of deoxynucleotides at the 3'-hydroxyl terminus of DNA, accompanied by the release of inorganic phosphate. TdT does not require a template and will not copy one. Reaction conditions...
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PMID: 18972388
PDF is available here.
PDF is available here.
médecine/sciences (24)10 2008
Abstract
We explore these notions, discuss the evidence in support of this theory, the implications, and some of the mechanisms which could explain this phenomenon....
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PMID: 18950581
PDF is available here.
PDF is available here.
Nucleic Acids Research (36)17 2008
Abstract
We show how a new single molecule PCR (smPCR)-based procedure can be employed as a general substitute to in vivo cloning thereby allowing for the first time in vitro DNA synthesis. We integrated this rapid and high fidelity in vitro procedure into our earlier recursive DNA synthesis and error correc...
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PMID: 18667587
PDF is available here.
PDF is available here.
Abstract
We exploit a sensitized human primary cell assay system to investigate the biological function of disease-linked telomerase RNA variants and their impact on the function of coexpressed wild-type telomerase RNA. We find that telomerase RNA variants discovered in patients with dyskeratosis congenita o...
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PMID: 18710936
PDF is available here.
PDF is available here.
ChemBioChem (9)14 2008
Abstract
Molecular Cell (31)6 2008
Abstract
We demonstrate that homing occurs through a TR-containing RNA intermediate and is RecA independent. Marker transfer studies show that cDNA integration at the 3' end of VR occurs within a (G/C)(14) element, and deletion analysis demonstrates that the reaction is independent of 5' end cDNA integration...
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PMID: 18922465
PDF is available here.
PDF is available here.
Biochemistry (Washington) (47)38 2008
Abstract
We recently demonstrated that this reaction can be coupled with upstream generation of trigger oligonucleotides from a genomic target sequence, and with downstream visual detection using DNA-functionalized gold nanospheres. The utility of EXPAR in clinical diagnostics is, however, limited by a nonsp...
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PMID: 18729381
PDF is available here.
PDF is available here.
Human Molecular Genetics (17)18 2008
Abstract
We analyze microindels in human cancers using an endogenous and transcribed gene, the TP53 gene. Microindel frequency, the enhancement of 1-2 microindels and other features are generally similar to that observed in the non-transcribed lacI gene in normal mouse tissues. The current larger sample of s...
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PMID: 18632684
PDF is available here.
PDF is available here.
Journal of Molecular Biology (381)4 2008
Abstract
We found that approximately 80% of strand gaps were repaired by physical strand transfer from the donor, whereas approximately 20% appear to be repaired by template switching. HR gap repair operated on both small and bulky lesions and largely depended on RecA and RecF but not on the RecBCD nuclease....
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PMID: 18585391
PDF is available here.
PDF is available here.
BioTechniques (45)3 2008
Abstract
We report on an unexpectedly high rate of unreadable chromatograms from plasmid sequencing using Beckman Coulter's protocols, chemistry, and CEQ8000 instrument. Failed or poor quality plasmid sequence chromatograms were accompanied by a sharp drop, fluctuation, or steady decline in the current and a...
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PMID: 18778257
PDF is available here.
PDF is available here.
Journal of Molecular Biology (381)3 2008
Abstract
Archaeal family B DNA polymerases bind tightly to template-strand uracil and stall replication on encountering the pro-mutagenic base. This article describes an X-ray crystal structure, at 2.8 A resolution, of Thermococcus gorgonarius polymerase in complex with a DNA primer-template containing uraci...
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PMID: 18614176
PDF is available here.
PDF is available here.
Journal of Virology (82)17 2008
Abstract
We constructed cell lines expressing the civet ortholog (DBT-cACE2) of the SARS-CoV receptor (hACE2). Zoonotic SARS-CoV was completely dependent on ACE2 for entry. Urbani grew with similar kinetics in both the DBT-cACE2 and the DBT-hACE2 cells, while icSZ16-S only grew in DBT-cACE2 cells. The SZ16-S...
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PMID: 18579604
PDF is available here.
PDF is available here.
Journal of Virology (82)18 2008
Abstract
We show that this enzyme also catalyzes nontemplated nucleotide additions to single-stranded and double-stranded nucleic acid molecules. This terminal nucleotidyltransferase activity not only is a property of the isolated enzyme but also is detected to take place within the viral nucleocapsid. This...
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PMID: 18614640
PDF is available here.
PDF is available here.
Nucleic Acids Research (36)16 2008
Abstract
We identify and characterize the novel-type promoter recognized by a group 3 sigma-factor, SigF. SigF autoregulates its own transcription and recognizes the promoter of pilA1 that acts in pilus formation and motility in PCC 6803. The pilA1 promoter (PpilA1-54) was recognized only by SigF and not by...
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PMID: 18689440
PDF is available here.
PDF is available here.
Abstract
We have cloned the cDNA for the mtRNAP of Physarum and have expressed the mtRNAP in Escherichia coli. We have used in vitro transcription assays based on the Physarum mtRNAP to identify a novel activity associated with the mtRNAP in which non-DNA-templated nucleotides are added to the 3' end of RNAs...
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PMID: 18573885
PDF is available here.
PDF is available here.
Abstract
We describe a completely defined in vitro system for transcription of a human tRNA gene assembled into a chromatin template. Transcriptional activation and histone acetylation in this system depend on recruitment of p300 by general initiation factor TFIIIC, thus providing a new paradigm for recruitm...
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PMID: 18644873
PDF is available here.
PDF is available here.
Nucleic Acids Research (36)16 2008
Abstract
We investigated whether adaptations for growth at high temperatures would alter a key functional component of the replication machinery, specifically DnaG primase. Although the structure of bacterial primases is conserved, the trinucleotide initiation specificity for A. aeolicus was hypothesized to...
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PMID: 18684998
PDF is available here.
PDF is available here.
Analytical Biochemistry (380)1 2008
Abstract
We have proposed mechanisms that underlie the supercomplex formation process. Whereas supercomplexes develop naturally in free solution, spatial constraints involved in a topologically limited system where template DNA is bound to the surface may facilitate the assembling process by stalling transcr...
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PMID: 18570883
PDF is available here.
PDF is available here.
Biomedical Microdevices (10)4 2008
Abstract
We developed a novel technique for fluorescent imaging of LAMP at a single molecule level. The experiment was conducted in a polyacrylamide (PAA) gel-based microchamber where a single DNA template, freely suspended in a solution containing primers and polymerase was initially encapsulated. In order...
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PMID: 18302022
PDF is available here.
PDF is available here.
2'-5' oligoadenylate synthetase shares active site architecture with the archaeal CCA-adding enzyme.
Abstract
We compared the structure of OAS to that of poly adenosine polymerase (PAP) and the class I CCA-adding enzyme from Archeoglobus fulgidus (AfCCA). This comparison revealed a strong structural homology between the three enzyme families. In particular, the active sites of OAS and CCA class I enzymes ar...
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PMID: 18604630
PDF is available here.
PDF is available here.