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Mutagenesis, Insertional (0):
Recent article
Mutagenesis, Insertional
Abstract
The dipteran Chironomus riparius is found across the entire Palearctic region; its larvae are among the most abundant macroinvertebrates inhabiting inland waterbodies. Chironomid larvae have been extensively used in ecotoxicological and cytogenetic research, but relatively little is...
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PMID: 21901555
PDF is available here.
PDF is available here.
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Developmental Cell (21)2 2011
Abstract
We show that selective autophagy downregulates Ty1 transposition by eliminating Ty1 VLPs from the cytoplasm under nutrient-limited conditions. Ty1 VLPs are targeted to autophagosomes by an interaction with Atg19. We propose that selective autophagy safeguards genome integrity against excessive inser...
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PMID: 21839922
PDF is available here.
PDF is available here.
Abstract
We test this hypothesis by comparing vulva induction in two genetically tractable nematodes, Caenorhabditis elegans and Pristionchus pacificus. Previous work indicated that the vulva is induced by epidermal growth factor (EGF)/RAS and WNT signaling in Caenorhabditis and Pristionchus, respectively. H...
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PMID: 21814488
PDF is available here.
PDF is available here.
Abstract
We briefly outline the current advances in somatic mutagenesis and discuss how these new tools, especially the piggyBac transposon system, can be applied to decipher human biology and disease....
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PMID: 21814514
PDF is available here.
PDF is available here.
Phytopathology (101)6 2011
Abstract
We have previously demonstrated that, although the Erwinia amylovora rcsC mutant produces more amylovoran than the wild-type (WT) strain in vitro, the mutant remains nonpathogenic on both immature pear fruit and apple plants. In this study, we have comparatively characterized the Erwinia RcsC and it...
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PMID: 21261468
PDF is available here.
PDF is available here.
Abstract
We ascertained a large Chinese family with an X-linked congenital hypertrichosis syndrome combining CGH, scoliosis, and spina bifida and mapped the disease locus to a 5.6 Mb critical region within the interval defined by the previously reported Mexican family. Through the combination of a high-resol...
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PMID: 21636067
PDF is available here.
PDF is available here.
FEMS Yeast Research (11)3 2011
Abstract
We isolated P. guilliermondii mutants overproducing riboflavin. Analysis of nucleotide sequence of recombination sites revealed that insertion cassettes integrated into the genome disrupting P. guilliermondii genes similar to the VMA1 gene of Ashbya gossypii and Saccharomyces cerevisiae and FES1 and...
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PMID: 21261808
PDF is available here.
PDF is available here.
Abstract
We provide the first genome sequence for S. agalactiae isolated from a cow diagnosed with clinical mastitis (strain FSL S3-026). Comparison to eight S. agalactiae genomes obtained from human disease isolates revealed 183 genes specific to the bovine strain. Subsequent polymerase chain reaction (PCR)...
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PMID: 21536150
PDF is available here.
PDF is available here.
Abstract
We describe a novel gene involved in adherence to epithelial cells. Transformation of C. diphtheriae 225, biotype gravis, ribotype St-Petersburg by EZ:TN(KAN-2)Tnp Transposome was undertaken. A C. diphtheriae 225 Tn5 insertion library of 2800 mutants was created. Five hundred and eighty five transfo...
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PMID: 21111065
PDF is available here.
PDF is available here.
Abstract
We constructed a Salmonella mutant strain in which gidA was deleted. Deletion of gidA rendered Salmonella deficient in the invasion of intestinal epithelial cells, bacterial motility, intracellular survival, and induction of cytotoxicity in host cells. Deletion of gidA rendered the organism to displ...
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PMID: 21320585
PDF is available here.
PDF is available here.
Abstract
Development of a method of transforming Aspergillus niger conidiospores with Agrobacterium tumefaciens T-DNA system and using it as a tool for genome annotation via construction a T-DNA insertion mutant library of A. niger.
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PMID: 21574390
PDF is available here.
PDF is available here.
Abstract
We designed and conducted genome-wide microarray analyses to characterize the HrpG and HrpX regulons, which are critical for the pathogenicity of X. axonopodis pv. citri. Our analyses revealed that 232 and 181 genes belonged to the HrpG and HrpX regulons, respectively. In total, 123 genes were overl...
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PMID: 21261465
PDF is available here.
PDF is available here.
Abstract
We conduct an in silico analysis of the distribution and abundance of the full complement of 1045 copies in the updated AgamP3 assembly. Chromosomal distribution of Ty3/gypsy elements is inversely related to arm length, with densities being greatest on the X, and greater on the short versus long arm...
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PMID: 21283637
PDF is available here.
PDF is available here.
Abstract
Brucellosis is a zoonosis caused by Brucella spp., a group of highly homogeneous bacteria. The insertion sequence IS711 is characteristic of these bacteria, and occurs in variable numbers and positions, but always constant within a given species. This species-associated polymorphism is used in molecu...
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PMID: 21813003
PDF is available here.
PDF is available here.
Abstract
We examined fitness consequences and genetic basis of variation in trichome production in a natural population of Arabidopsis halleri subsp. gemmifera. Half of the individuals in the study population produced trichomes while the other half were glabrous, and the leaf beetle Phaedon brassicae imposed...
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PMID: 21811571
PDF is available here.
PDF is available here.
Abstract
We insertionally inactivated the ccp gene C. perfringens strain 13 using TargeTron technology, constructing a strain that was no longer proteolytic on skim milk agar. Quantitative protease assays confirmed the absence of extracellular protease activity, which was restored by complementation with the...
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PMID: 21829506
PDF is available here.
PDF is available here.
Abstract
We defined the important residues and domains of the Arabidopsis YUC1 FMO, a key enzyme in auxin biosynthesis. We previously showed that simultaneous inactivation of YUC1 and its homologue YUC4 caused severe defects in vascular and floral development. We mutagenized the yuc4 mutant and screened for...
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PMID: 21205174
PDF is available here.
PDF is available here.
Methods in Cell Biology (104) 2011
Abstract
We describe both autonomous and non-autonomous transposable elements, and we compare and contrast popular transposon systems. The Tol2 system is a robust gene transfer tool and has been selected as the primary transposon platform, facilitating the development of an array of reagents readily shared w...
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PMID: 21924161
PDF is available here.
PDF is available here.
Abstract
We explored the chromosomally-encoded determinants that contribute to the phenotype of susceptibility of P. aeruginosa to ceftazidime, imipenem and carbapenem. Using a comprehensive library of transposon-tagged insertion mutants, we found 37 loci in the chromosome of P. aeruginosa that contributed t...
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PMID: 21304266
PDF is available here.
PDF is available here.
American Surgeon (11) 2011
Abstract
Chemical Communications (46)40 2010
Abstract
Ferrocene-modified DNA probes formed fully matched duplexes and bulge-containing ones with wild-type and insertion/deletion-type complements of clinical importance, respectively. Cyclic voltammetry measurements revealed that the bulge-containing duplexes showed an increased flexibility compared to t...
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PMID: 20852766
PDF is available here.
PDF is available here.
Abstract
We report the development of a suite of six integrative vectors for construction of single copy transcriptional fusions with the gfpmut3, cfp and iyfp reporter genes in Bacillus subtilis. The promoter fusions are constructed using the highly efficient ligation-independent cloning (LIC) technique mak...
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PMID: 20600285
PDF is available here.
PDF is available here.
Abstract
We describe the generation of an inducible Cre transgenic line allowing conditional mutagenesis in ovarian granulosa cells. We have expressed the tamoxifen inducible CreER(T)² fusion protein from a Bacterial Artificial Chromosome (BAC) containing the regulatory elements of the hydroxysteroid (17-be...
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PMID: 20715176
PDF is available here.
PDF is available here.
Abstract
We evaluated this method of mutation screening for the two major breast and ovarian cancer susceptibility genes BRCA1 and BRCA2. Screening of these two genes is time-consuming and must include exploration of large rearrangements that represent 5% to 15% of the alterations observed in these genes. To...
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PMID: 20858050
PDF is available here.
PDF is available here.
Abstract
We used a recently developed indel-simulation program to examine the false-positive rate and power of the branch-site test. We find that insertions and deletions do not cause excessive false positives if the alignment is correct, but alignment errors can lead to unacceptably high false positives. Of...
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PMID: 20447933
PDF is available here.
PDF is available here.
Human Molecular Genetics (19)R2 2010
Abstract
We focus on progress that has been made with detecting small insertions and deletions (INDELs) in human genomes. Over the past decade, several million small INDELs have been discovered in human populations and personal genomes. The amount of genetic variation that is caused by these small INDELs is...
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PMID: 20858594
PDF is available here.
PDF is available here.
Annals of Medicine (42)6 2010
Abstract
Defects of the 11betaHSD2 gene do not constitute a likely cause for licorice-induced hypertension. Variants of the ENaC subunits may render some individuals sensitive to licorice-induced metabolic alterations and hypertension....
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PMID: 20597806
PDF is available here.
PDF is available here.
Abstract
I sequence of ebl-1 gene in order to investigate polymorphism in the region immediately adjacent to the 5' cysteine-rich domains, and to determine the prevalence of an insertion mutant that effectively knocks out the gene.
Copyright 2009 Elsevier B.V. All rights reserved....
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PMID: 19879379
PDF is available here.
PDF is available here.
Journal of Virology (84)16 2010
Abstract
The highly pathogenic avian influenza (HPAI) virus phenotype is restricted to influenza A viruses of the H5 and H7 hemagglutinin (HA) subtypes. To obtain more information on the apparent subtype-specific nature of the HPAI virus phenotype, a low-pathogenic avian influenza (LPAI) H6N1 virus was gener...
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PMID: 20519405
PDF is available here.
PDF is available here.
Journal of Virology (84)16 2010
Abstract
We performed random mutagenesis of PrV pUL36 by transposon-mediated insertion of a 15-bp linker. By this approach, 26 pUL36 insertion mutants were selected and tested in transient transfection assays for their ability to complement one-step growth and/or viral spread of a PrV UL36 null mutant. Ten i...
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PMID: 20534865
PDF is available here.
PDF is available here.
Nucleic Acids Research (38)14 2010
Abstract
We have created a universal, sequence-verified TagModule collection. A hallmark of the 4280 TagModules is that they are cloned into a Gateway entry vector, thus facilitating rapid transfer to any compatible genetic system. Here, we describe the application of the TagModules to rapidly generate tagge...
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PMID: 20494978
PDF is available here.
PDF is available here.
Infection and Immunity (78)8 2010
Abstract
We have identified several loci that are required for C. jejuni efficient entry and survival within epithelial cells. Among these loci, insertional mutations in aspA, aspB, and sodB resulted in drastic reduction in C. jejuni entry and/or survival within host cells and a severe defect in colonization...
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PMID: 20515930
PDF is available here.
PDF is available here.
Abstract
T-DNA insertional mutagenesis is one of the most important approaches for gene discovery and cloning. A fertile polyembryo mutant generated by T-DNA/Ds insertion in Oryza sativa, cv. Basmati 370 showed twin or triple seedlings at a frequency of 15-20%. T-DNA insertion was confirmed by 950 bp hpt gen...
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PMID: 19816724
PDF is available here.
PDF is available here.
Abstract
A dwarf mutant (Oryza sativa anaphase-promoting complex 6 (OsAPC6)) of rice cultivar Basmati 370 with 50% reduced plant height as compared to the wild type was isolated by Agrobacterium tumefaciens-mediated transformation using Hm(R) Ds cassette. This mutant was found to be insensitive to exogenous...
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PMID: 20091079
PDF is available here.
PDF is available here.
Abstract
A collection of 4117 fertile T-DNA lines has been generated by Agrobacterium-mediated transformation of the diploid community standard line Bd21 of Brachypodium distachyon. The regions flanking the T-DNA left and right borders of the first 741 transformed plants were isolated by adap...
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PMID: 20374523
PDF is available here.
PDF is available here.
FEMS Microbiology Letters (309)1 2010
Abstract
The Lancefield group C alpha-hemolytic Streptococcus dysgalactiae ssp. dysgalactiae (GCSD) causes systemic granulomatous inflammatory disease and high mortality rates in infected fish. Superantigen and streptolysin S genes are the most important virulence factors contributing to an i...
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PMID: 20528946
PDF is available here.
PDF is available here.
Abstract
We surveyed in this study.
Published by Elsevier Masson SAS....
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PMID: 20554213
PDF is available here.
PDF is available here.
Journal of Bacteriology (192)15 2010
Abstract
We show that the foci are due to OmpR-YFP (yellow fluorescent protein) fusion binding to specific sites in the chromosome. To identify positions of foci and quantify their fluorescence intensity, we used a simple system to tag virtually any chromosomal location with arrays of lacO or tetO. The brigh...
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PMID: 20511496
PDF is available here.
PDF is available here.
Abstract
We therefore examined chromosomes with insertions and found that they preferentially segregated away from the X chromosome during meiosis in Caenorhabditis elegans males. Conversely, chromosomes with deletions preferentially segregated with the X chromosome. The degree of segregation bias was signif...
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PMID: 20647459
PDF is available here.
PDF is available here.
Plant Physiology (153)3 2010
Abstract
We identify a new gene, UPRIGHT ROSETTE (URO), the overexpression of which alters IAA homeostasis in Arabidopsis (Arabidopsis thaliana). We previously identified a semidominant mutant, uro, which had multiple auxin-related phenotypes. We show here that compared to wild-type plants, the uro plants co...
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PMID: 20466845
PDF is available here.
PDF is available here.
Plant Physiology (153)3 2010
Abstract
We show that these isozymes are encoded by five genes in Arabidopsis (Arabidopsis thaliana). Alternative translation initiation or alternative splicing of CDS2 and CDS4 transcripts can result in up to 10 isoforms. Most of the cDNAs encoding the various plant isoforms were functionally expressed in y...
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PMID: 20442275
PDF is available here.
PDF is available here.
Plant Physiology (153)3 2010
Abstract
We conclude that GR1 plays a crucial role in daylength-dependent redox signaling and that this function cannot be replaced by the second Arabidopsis GR gene or by thiol systems such as the thioredoxin system....
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PMID: 20488891
PDF is available here.
PDF is available here.
Plant Physiology (153)3 2010
Abstract
Our results confirm that AtPAP26 is a principal contributor to Pi stress-inducible APase activity, and that it plays an important role in the Pi metabolism of -Pi Arabidopsis....
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PMID: 20348213
PDF is available here.
PDF is available here.
Abstract
We have shown using two-dimensional gel electrophoresis (2-DE) that several proteins, including a transcription regulator, FMN-binding protein, major facilitator superfamily permease, glycogen phosphorylase, the YknV protein, and fructose/tagatose bisphosphate aldolase, were strongly regulated by th...
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PMID: 20495044
PDF is available here.
PDF is available here.
Abstract
We adapted the FLP/FRT recombination system from the yeast Saccharomyces cerevisiae for marker recycling. We tested this system in the penicillin producer Penicillium chrysogenum using different experimental approaches. In a two-step application, we first integrated ectopically a nourseothricin resi...
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PMID: 20472720
PDF is available here.
PDF is available here.
Abstract
We report evidence of natural genetic competence in 13 Thermoanaerobacter and Thermoanaerobacterium strains previously believed to be difficult to transform or genetically recalcitrant. In Thermoanaerobacterium saccharolyticum JW/SL-YS485, natural competence-mediated DNA incorporation occurs during...
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PMID: 20472726
PDF is available here.
PDF is available here.
Abstract
We investigated neurogenesis in a hypomorphic mouse model for the TGF-beta and BMP inhibitor Smad7, with the hypothesis that NPC proliferation might be reduced due to increased TGF-beta and BMP signaling. Unexpectedly, we found enhanced NPC proliferation as well as an increased number of label-retai...
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PMID: 20479122
PDF is available here.
PDF is available here.
Hereditas (Beijing) (32)7 2010
Abstract
We detected 10 HLA-A allele sequences 4.2 kb in length and 6 HLA-B allele sequences 3.7 kb in length, and the sequences included all exons, all introns, 5'promoter, and 3'UTR of the two genes. All sixteen sequences have been submitted to GenBank and IMGT/HLA database. A*1153 is a novel allele, and t...
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PMID: 20650849
PDF is available here.
PDF is available here.
New Phytologist (188)2 2010
Abstract
We used the Arabidopsis seed coat to study the participation of the exocyst complex in polarized pectin delivery. • We characterized the amount of pectinaceous mucilage and seed coat structure in sec8 and exo70A1 exocyst mutants. Using a yeast two-hybrid screen, we identified a new interactor of t...
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PMID: 20618910
PDF is available here.
PDF is available here.
Infection and Immunity (78)7 2010
Abstract
We wanted to individually evaluate putative virulence determinants suggested by such assays and performed directed screening of 249 F. novicida transposon insertion mutants by using survival of infected fruit flies as a measure of bacterial virulence. Some 20% of the genes tested were required for n...
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PMID: 20479082
PDF is available here.
PDF is available here.
Abstract
We investigated the role of ligand dimensions in regulation of mouse NK-cell activation and inhibition. Elongated forms of H60a, a mouse NKG2D ligand, were generated and expressed stably in the RMA cell line. RMA cells expressing the normal size H60a were lysed efficiently by both freshly isolated a...
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PMID: 20432238
PDF is available here.
PDF is available here.
DNA and Cell Biology (29)7 2010
Abstract
We carried out a case-control study in a Chinese population (206 cases and 302 controls) to estimate the susceptibility to HCC associated with an 18-bp insertion/deletion polymorphism (rs35569394) in the promoter region of VEGF. After adjusting the data by gender, age, smoking status, drinking statu...
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PMID: 20406100
PDF is available here.
PDF is available here.
Plant Journal (63)1 2010
Abstract
We investigated the functioning of OsCOL4, a member of the CONSTANS-like (COL) family in rice. OsCOL4 null mutants flowered early under short or long days. In contrast, OsCOL4 activation-tagging mutants (OsCOL4-D) flowered late in either environment. Transcripts of Ehd1, Hd3a, and RFT1 were increase...
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PMID: 20409004
PDF is available here.
PDF is available here.
Plant Journal (63)1 2010
Abstract
We describe a new method that can substantially increase the throughput of linking a disrupted gene to a known phenotype in high-copy Mutator (Mu) transposon lines in maize. The approach uses the Illumina platform to obtain sequences flanking Mu elements in pooled, bar-coded DNA samples. Insertion s...
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PMID: 20409008
PDF is available here.
PDF is available here.
Plant Journal (63)1 2010
Abstract
We investigated the functioning of OsCOL4, a member of the CONSTANS-like (COL) family in rice. OsCOL4 null mutants flowered early under short or long days. In contrast, OsCOL4 activation-tagging mutants (OsCOL4-D) flowered late in either environment. Transcripts of Ehd1, Hd3a, and RFT1 were increase...
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PMID: 20409004
PDF is available here.
PDF is available here.
Plant Journal (63)1 2010
Abstract
We investigated the functioning of OsCOL4, a member of the CONSTANS-like (COL) family in rice. OsCOL4 null mutants flowered early under short or long days. In contrast, OsCOL4 activation-tagging mutants (OsCOL4-D) flowered late in either environment. Transcripts of Ehd1, Hd3a, and RFT1 were increase...
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PMID: 20409004
PDF is available here.
PDF is available here.
Plant Journal (63)1 2010
Abstract
We investigated the functioning of OsCOL4, a member of the CONSTANS-like (COL) family in rice. OsCOL4 null mutants flowered early under short or long days. In contrast, OsCOL4 activation-tagging mutants (OsCOL4-D) flowered late in either environment. Transcripts of Ehd1, Hd3a, and RFT1 were increase...
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PMID: 20409004
PDF is available here.
PDF is available here.
Plant Journal (63)1 2010
Abstract
Thylakoid biogenesis is a crucial step for plant development involving the combined action of many cellular actors. CPSAR1 is shown here to be required for the normal organization of mature thylakoid stacks, and ultimately for embryo development. CPSAR1 is a chloroplast protein that...
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PMID: 20408996
PDF is available here.
PDF is available here.
Plant Journal (63)1 2010
Abstract
Thylakoid biogenesis is a crucial step for plant development involving the combined action of many cellular actors. CPSAR1 is shown here to be required for the normal organization of mature thylakoid stacks, and ultimately for embryo development. CPSAR1 is a chloroplast protein that...
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PMID: 20408996
PDF is available here.
PDF is available here.
Plant Journal (63)1 2010
Abstract
Thylakoid biogenesis is a crucial step for plant development involving the combined action of many cellular actors. CPSAR1 is shown here to be required for the normal organization of mature thylakoid stacks, and ultimately for embryo development. CPSAR1 is a chloroplast protein that...
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PMID: 20408996
PDF is available here.
PDF is available here.