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Articles on Ploidies
CycA is involved in the control of endoreplication dynamics in the Drosophila bristle lineage.
We focused on CycA, which has been described to function exclusively during mitosis in Drosophila. Using developing mechanosensory organs as model system and PCNA::GFP to follow endocycle dynamics, we show that (1) CycA proteins accumulate during the last period of endoreplication, (2) both CycA los...
Centrosomal dysregulation in human metastatic melanoma cell lines.
We characterized the centrosomal status, ploidy, and gene status (TP53, CDKN2A/B, BRAF, and NRAS) of 15 human metastatic melanoma cell lines. Cells were labelled for pericentrin (a centrosomal marker), DNA and α-tubulin, and scored for centrosome morphology, supernumerary centrosomes, and mitotic s...
[Comparative study on photosynthetic characteristics of different ploidy Rhodiola sachalinensis germplasm].
To compare the photosynthetic characteristics difference of different ploidy Rhodiola sachalinensis germplasm and provide the scientific basis for their cultivation.
DNA ploidy is stronger than lymph node metastasis as prognostic factor in cervical carcinoma: 10-year results of a prospective study.
We investigated whether the presence of lymph node metastasis may have a different clinical impact according to ploidy status. In a long-term prospective study, DNA ploidy was evaluated by flow cytometry from fresh tumor samples from 136 patients with cervical cancer who underwent su...
Bovine parthenogenotes produced by inhibition of first or second polar bodies emission.
We propose a new strategy to produce bovine parthenogenetic embryos inhibiting the emission of the first polar body during in vitro maturation, and allowing the extrusion of the second polar body during oocyte activation. Cytochalasin B, an inhibitor of actin microfilaments, was employed during in v...
Unisexual reproduction among vertebrates
The past decade has seen a remarkable revision of perspectives on unisexual reproduction in vertebrates. One can no longer view it as a rare curiosity far outside the mainstream of evolution. More than 80 taxa of fish, amphibians, and reptiles are now known to reproduce by parthenogenesis (Greek for...
Ploidy of cell-sorted trophic and cystic forms of Pneumocystis carinii.
Once regarded as an AIDS-defining illness, Pneumocystis pneumonia (PcP) is nowadays prevailing in immunocompromised HIV-negative individuals such as patients receiving immunosuppressive therapies or affected by primary immunodeficiency. Moreover, Pneumocystis clinical spectrum is bro...
Correlation between DNA ploidy, metaphase high-resolution comparative genomic hybridization results and clinical outcome of synovial sarcoma...
We found significant correlation between the different ploidy groups and the clinical outcome (p < 0.05)....
[The importance of DNA-cytometry for the prediction of nasopharyngeal malignancy].
The method of DNA-cytometry was applied to examine patients presenting with nasopharyngeal malignancy. The results obtained in laboratory studies were compared with clinical observations and data of medical histories. It was shown that diploid and aneuploid nasopharyngeal tumours are represented in a...
A physical map for the Amborella trichopoda genome sheds light on the evolution of angiosperm genome structure.
When placed in the context of a physical map, BAC end sequences representing just 5.4% of the Amborella genome have facilitated reconstruction of gene blocks that existed in the last common ancestor of all flowering plants. The Amborella genome is an invaluable reference for inferences concerning th...