Abstract
We will characterize the epidemiology and global expansion of CHIKV, describe the clinical features and laboratory testing for the disease, and discuss priorities for further studies needed for effective disease control and prevention....
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PMID: 19663604
PDF is available here.
Abstract
Our results confirmed that CAV is still circulating in commercial flocks in Argentina. Phylogenetic analysis focusing on the VP1 nucleotide sequence showed that all Argentinean isolates grouped together in a cluster, sharing a high similarity (> 97%) with genotype B reference strains. However, Argen...
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PMID: 19848068
PDF is available here.
Abstract
Staphylococcus aureus is the leading cause of bacterial infections in developed countries and produces a wide spectrum of diseases, ranging from minor skin infections to fatal necrotizing pneumonia. Although S. aureus infections were historically treatable with common antibiotics, emergence of drug-...
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PMID: 19729844
PDF is available here.
Abstract
I use case studies of 3 RNA viruses to explore the impact of genomic sequence data, with particular emphasis on influenza A virus. Notably, the studies of RNA virus genomics undertaken to date largely focused on issues of evolution and epidemiology, and they have given these disciplines new impetus....
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PMID: 19729846
PDF is available here.
Abstract
We provide examples of the progress made by exploiting an integrated genome-wide research platform to gain new insight into molecular pathogenesis. The studies have provided many new avenues for basic and translational research....
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PMID: 19729843
PDF is available here.
J Joukje JJ Siebenga,
Harry H Vennema,
Du-Ping DP Zheng,
Jan J Vinjé,
Bonita E BE Lee,
Xiao-Li XL Pang,
Eric C M EC Ho,
Wilina W Lim,
Avinash A Choudekar,
Shobha S Broor,
Tamar T Halperin,
Nassar B G NB Rasool,
Joanne J Hewitt,
Gail E GE Greening,
Miao M Jin,
Zhao-Jun ZJ Duan,
Yalda Y Lucero,
Miguel M O'Ryan,
Marina M Hoehne,
Eckart E Schreier,
Rodney M RM Ratcliff,
Peter A PA White,
Nobuhiro N Iritani,
Gábor G Reuter and
Marion M Koopmans
Abstract
NoV epidemics resulted from the global spread of GII.4 strains that evolved under the influence of population immunity. Lineages show notable (and currently unexplained) differences in geographic prevalence. Establishing a global NoV network by which data on strains with the potential to cause pande...
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PMID: 19627248
PDF is available here.
Abstract
Hepatitis B virus (HBV) is a public health problem worldwide, and apart from infecting humans, HBV has been found in nonhuman primates. This study investigated the prevalence and phylogenetic analysis of hepatitis B virus (HBV) and hepatitis D virus (HDV) among nonhuman primates in Taiwan, an area w...
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PMID: 19746868
PDF is available here.
Abstract
We optimized a PFGE protocol for subtyping of Acinetobacter baumannii, Escherichia coli and Klebsiella spp., which are commonly isolated from nosocomial infections in many hospitals. Reproducibility of our PFGE procedure was studied three times at 2- to 3-week intervals. Epidemiological concordance...
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PMID: 19762987
PDF is available here.
Abstract
We concluded that ERIC-PCR is not suitable for differentiating isolates within the same serotype and for grouping into clusters. In PFGE using the AvrII enzyme, S. Choleraesuis isolates formed three clusters, and S. Hadar isolates formed three clusters; using the XbaI enzyme, S. Choleraesuis isolate...
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PMID: 19762985
PDF is available here.
Abstract
A novel genotyping method for methicillin-resistant Staphylococcus aureus (MRSA), the phage open-reading frames typing (POT) method, was evaluated using 92 MRSA isolates collected from blood cultures between 1991 and 2003 at Nagoya University Hospital. These strains were divided into 64 distinct POT...
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PMID: 19762991
PDF is available here.
Abstract
This is the first comprehensive molecular epidemiological study of HIV-1 CRF01_AE in Hong Kong. It revealed that MSM contact is becoming a major route of local CRF01_AE transmission in Hong Kong. Epidemiological linkage of CRF01_AE between Hong Kong and China observed in this study indicates the imp...
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PMID: 19521252
PDF is available here.
Abstract
Our aim has been to evaluate the genetic diversity of 40 H. pylori clinical isolates from four different parts of China. The methods of multi-locus sequence typing and vacA allele genotyping were used to assess their genetic diversity. To discriminate MLST, the vacA genotype method was used to ident...
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PMID: 19452214
PDF is available here.
Abstract
In 2007, an outbreak of acute hemorrhagic conjunctivitis (AHC) occurred in Beijing. In order to identify the etiology of this outbreak, 57 eye conjunctival swabs were collected from 57 outpatient patients, and detected for adenovirus, human enterovirus 70 (HEV70) and Coxsackievirus A24 variant (CVA2...
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PMID: 19769156
PDF is available here.
Abstract
In the last decade, a declining prevalence of HCV infection has been described in injecting drug users (IDUs) in different countries. This study is the first to assess temporal trends in drug-injecting patterns, HCV infection rates and viral genotype distribution in 770 Brazilian IDUs, recruited by...
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PMID: 19144250
PDF is available here.
Abstract
I of the IARS; of these, 1,195 individuals from 220 families were included in the heritability analysis. With the exception of leptin, all phenotypes exhibited significant age- and sex-adjusted heritability (p < 0.0001). Amongst all the phenotypes analysed after adjustment for confounding factors, t...
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PMID: 19652890
PDF is available here.
Abstract
A total of 52 methicillin-resistant Staphylococcus aureus (MRSA) clinical isolates were collected from patients attending the teaching hospital of Tehran University of Medical Sciences. Disks containing antibiotics were used to determine the susceptibility of MRSA isolates. Analysis of SmaI macrores...
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PMID: 19628913
PDF is available here.
Abstract
We diagnosed 10 patients with CHIKV infection and 5 with dengue virus infection. Joint pain is a significant symptom of chikungunya fever. Five strains of CHIKV were isolated. Their genome sequences were different from those isolated from the previous outbreaks in Thailand (1988, 1995-1996) but simi...
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PMID: 19628911
PDF is available here.
Abstract
Scedosporium species are increasingly encountered opportunistic fungal pathogens not only in immunocompromised patients but are also significant primary pathogens in immunocompetent individuals. The environmental reservoir of these fungi is uncertain and the epidemiology and mode of transmissi...
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PMID: 19085455
PDF is available here.
Abstract
We describe protocols and bioinformatics tools that allow the identification of CRISPRs from sequenced genomes, their comparison, and their component determination (the direct repeats and the spacers). A schematic representation of the spacer organization can be produced, allowing an easy comparison...
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PMID: 19521870
PDF is available here.
Abstract
Multilocus sequence typing (MLST) was first proposed in 1998 as a typing approach that enables the unambiguous characterization of bacterial isolates in a standardized, reproducible, and portable manner using the human pathogen Neisseria meningitidis as the exemplar organism. Since then, the approac...
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PMID: 19521872
PDF is available here.
Abstract
We discuss the basic methodology for conducting IS6110-based RFLP and analyzing the resulting hybridization profiles....
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PMID: 19521875
PDF is available here.
Abstract
Since the 1980s the epidemiology of Clostridium difficile infection (CDI) has been investigated by the application of many different typing or fingerprinting methods. To study the epidemiology of CDI, a typing method with a high discriminatory power, typeability, and reproducibility is required. Mol...
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PMID: 19521874
PDF is available here.
Abstract
As the use of nucleotide sequence-based typing has become more widespread in the investigation of microbial epidemiology, there has been a natural requirement for curated Internet-based databases that can act as central authorities for nomenclature and type definitions. These facilitate the sharing...
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PMID: 19521882
PDF is available here.
Abstract
The use of molecular techniques in epidemiology gives a better understanding of viral transmission and diversity, and helps to define and characterizc outbreaks. By elucidating transmission patterns and defining outbreak parameters, appropriate preventive measures can be implemented in a timely fash...
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PMID: 19521877
PDF is available here.
Abstract
Genotyping of bacteria through typing of loci containing a variable number of tandem repeats (VNTR) might become the gold standard for many pathogens. The development of genome sequencing has shown that such sequences were present in every species analyzed, and that polymorphism exists in at least a...
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PMID: 19521873
PDF is available here.
Abstract
We are facing a revolution in DNA sequencing with many drastically different platforms that have become or will soon become available on the market. We review a number of sequencing technologies and provide examples of applications. We also discuss the impact genomics and new DNA sequencing approach...
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PMID: 19521879
PDF is available here.
Abstract
Nucleic acid sequencing is now commonplace in most research and diagnostic virology laboratories. The data generated can be used to compare novel strains with other viruses and allow the genetic basis of important phenotypic characteristics, such as antigenic determinants, to be elucidated. Furtherm...
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PMID: 19521878
PDF is available here.
Abstract
The spa typing method is based on sequencing of the polymorphic X region of the protein A gene (spa), present in all strains of Staphylococcus aureus. The X region is constituted of a variable number of 24-bp repeats flanked by well-conserved regions. This single-locus sequence-based typing method c...
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PMID: 19521876
PDF is available here.
Abstract
We elaborate on a recently described PCR-based typing method that streamlines the typing of plasmids occurring among the Enterobacteriaceae; we believe the method will prove applicable to the study of plasmids on a large scale....
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PMID: 19521864
PDF is available here.
Abstract
I expose the main properties and theoretical background of a somewhat out-of-fashion technique, multilocus enzyme electrophoresis (MLEE). I show that the remarkable properties of this marker-clear Mendelian inheritance, codominance, strong phylogenetic signal-are still valid, although of course more...
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PMID: 19521863
PDF is available here.
Dora Il'yasova,
Bridget J McCarthy,
Serap Erdal,
Joanna Shimek,
Jennifer Goldstein,
Daniel R Doerge,
Steven R Myers,
Paolo Vineis,
John S Wishnok,
James A Swenberg,
Darell D Bigner and
Faith G Davis
Abstract
This review is based on the proceedings from the Second Lebow Conference, held in Chicago in 2007. The conference concentrated on developing a framework for innovative studies in the epidemiology of environmental exposures, focusing specifically on the potential relationship with brain tumors. Resear...
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PMID: 19466671
PDF is available here.
Abstract
Thirty-three batches were contaminated by L. monocytogenes (33/1275, 2.6%), in which serovar 4b dominated (65.2%), followed by serovars 1/2b (17.4%), 1/2a (13.0%) and 1/2c (4.4%). These isolates were all as virulent as L. monocytogenes reference strains. Of 23 selected L. monocytogenes isolates and...
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PMID: 19673412
PDF is available here.
Abstract
Clostridium septicum is a highly virulent, anaerobic bacterium capable of establishing necrotizing tissue infections and forming heat resistant endospores. Disease is primarily facilitated by secretion of numerous toxic products including a lethal pore-forming cytolysin. Spontaneously occurring clos...
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PMID: 19402197
PDF is available here.
Abstract
In 2007 eight epidemic clusters (more than 15 cases in each) and other sporadic cases of measles occurred in Hokkaido district, Japan. A total of 850 cases were identified. Approximately half of them were > or = 15 years of age, resulting in a huge public health problem in the community associated w...
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PMID: 19468183
PDF is available here.
Abstract
The early and rapid detection and characterization of specific nucleic acids of medico-veterinary pathogens have proven invaluable for diagnostic purposes. The integration of amplification and signal detection systems, including online real-time devices, have increased speed and sensitivity and grea...
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PMID: 19435457
PDF is available here.
Abstract
Although extensive data are available on low pathogenic avian influenza (LPAI) virus surveillance in wild birds in North America and Europe, data are scarce for other parts of the world, and our understanding of LPAI virus ecology in the natural reservoir is still far from complete. The outbreak of...
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PMID: 19618618
PDF is available here.
Abstract
Cryptococcus neoformans frequently causes fungal meningitis in immunocompromised patients, whereas the related species C. gattii is restricted to tropical and subtropical regions,where it usually infects immunocompetent individuals.An outbreak of C. gattii infection that began in 1999 on Vancouver I...
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PMID: 19220140
PDF is available here.
Abstract
In Pakistan, the main causative agent in southern dry areas was determined as Leishmania major followed by Leishmania tropica. There was no correlation between the skin lesion types and the causative species. In the western Indian Himalayas, L. tropica and Leishmania donovani have been reported as t...
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PMID: 19276879
PDF is available here.
Abstract
Our proposed technique allows the identification of eight members of the vap gene family (vapA, B, -C, -D, -E, -F, -G, and -H). It is a practical and efficient method of conducting clinical and epidemiological studies on R. equi isolates....
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PMID: 19205798
PDF is available here.
Abstract
Using a standardised approach to explore themes around which individuals may have been exposed to TB resulted in the detection of previously unrecognised epidemiological links. Epidemiological data obtained from cluster investigations, e.g., risk and social behaviours that increase the risk of infec...
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PMID: 19335955
PDF is available here.
Abstract
In order to define hepatitis B virus (HBV) mutational patterns in Iran, nucleotide sequences obtained from 91 patients and encompassing the precore, basal core promoter (BCP) and surface (S) regions, were compared. The patients were grouped as asymptomatic carriers, chronic active hepatitis or cirrh...
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PMID: 19222745
PDF is available here.
Abstract
There have been relatively few studies on HCV genotype 6 compared to hepatitis C virus (HCV) genotype 1. In Hong Kong, a city of about 7 million people, most patients infected with HCV are infected with either genotype 1b or 6a. It is known that HCV 6 is of intermediate responsiveness (i.e., between...
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PMID: 19235848
PDF is available here.
Abstract
We describe a DNA microarray comprised of 282 sixty-mer oligonucleotide probes to study the epidemiology of Salmonella enterica subsp. enterica isolates at the genotypic level. The probes detect targets encoding genes associated with pathogenicity, antibiotic resistance, fimbriae, prophages, flagell...
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PMID: 19521880
PDF is available here.
Ji-Hua Zhou,
Hai-Lin Zhang,
Jing-Lin Wang,
Wei-Hong Yang,
Zhu-Qing Mi,
Yun-Zhi Zhang,
Yu-Zhen Zhang,
Xian-Yi Song,
Qiu-Ling Hu,
Ying-Kuan Dong,
Wen-Hua Pu,
Hai-Mei Hu,
Li-Fen Gao,
Qing-Hong Yuan,
Hong-Xiang Ya and
Yun Feng
Abstract
1421 rodents were captured and classified into 8 species of 4 Genera in the epidemic area within 10 counties of Chuxiong prefecture, Yunnan province, between 2005 and 2006. Out of the 1421 rodents, 1056 (74.31% ) of them were Rattus norvegicus and 280 (19.70%) belonged to Rattus flavipectus. The ant...
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PMID: 19642377
PDF is available here.
Abstract
A small but significant number of HIV-2 cases have been reported from India. Although all HIV-2 viruses identified belong to subtype A, there are no studies on the diversity of the HIV-2 envelope and other genomic regions from India. Furthermore, HIV-2 envelope quasispecies in infected individuals h...
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PMID: 19327056
PDF is available here.
Abstract
Twenty Newcastle disease virus (NDV) strains were isolated from chickens and geese in the field outbreaks during 2005 and 2006 in some regions of Jiangsu and Guangxi province. Assessment of the virulence by MDT and ICPI, RT-PCR and sequence analysis of fusion protein gene were used to compare the pr...
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PMID: 19678566
PDF is available here.
Abstract
The aim of this study was to determine the molecular epidemiology of cefoxitin-resistance Escherichia coli identified in cattle entering feedlots and determine if there were any similarities to E. coli causing human infections in Canadian hospitals. A total of 51 E. coli were isolated from a total o...
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PMID: 18824313
PDF is available here.
Abstract
The aim of this study was to address the prevalence and the molecular characteristics of antibiotic-resistant enteric bacteria isolated from one of the most popular types of Egyptian cheese. A total of 215 ampicillin-resistant enterobacterial isolates were obtained from 80 samples of Domiati cheese,...
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PMID: 19343954
PDF is available here.
Abstract
During a decade-long, high endemic situation with severe group A streptococcal disease in western Norway, a cluster of 16 patients with invasive streptococcal disease was hospitalized during a period of 11 weeks. A study including clinical characteristics and molecular epidemiology of the outbreak w...
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PMID: 19178544
PDF is available here.
Abstract
The prevalence rates of MTHFR and PT gene mutations were not significantly different between the groups. The prevalence rate of FV mutation was significantly higher in patients with arterial thrombosis. Coinheritance of FV and MTHFR was found in 67% of patients, which was significantly higher in art...
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PMID: 19353312
PDF is available here.
Abstract
This high-throughput, low cost method accurately determines CNV in the GST genes enabling reliable estimates of disease prediction in large epidemiological samples....
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PMID: 19026998
PDF is available here.
Abstract
We will discuss the epidemiology, clinical outcome and response to antiviral therapy for different hepatitis B virus genotypes....
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PMID: 19260575
PDF is available here.
Abstract
5 outbreaks of viral gastroenteritis caused by Norovirus were reported. A total of 63 stools were obtained from cases with acute gastroenteritis. Noroviruses alone were detected in 45 cases and the illness appeared in autumn. Phylogenetic analysis revealed that Norovirus belonged to G II/G II 4 type...
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PMID: 19565875
PDF is available here.
Abstract
A 30 month prospective study of Acinetobacter species encountered in the Central Pathology Laboratory of St James's Hospital, Dublin, Ireland, was conducted to investigate the prevalence and molecular epidemiology of carbapenem resistance in such isolates. Acinetobacter genomic species 3 (AG3) was f...
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PMID: 19141738
PDF is available here.