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Welcome to the Pubget Medical Subject Headings (MESH) browser. Click on a topic or subtopic below to explore related papers. In the gold box is the most recent paper about the current MESH term, and below are 20 related papers.
Biotin (0):
Recent article
Biotin
Abstract
In Wistar rats, specific inhibitor UPS lactacystin induced degeneration of 24% of the dopaminergic neurons in the black substance. The work shows that a moderate weakening of the UPS function is characterized by an enhanced activity of the shaperon system. This process seems to resto...
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PMID: 21961290
PDF is available here.
PDF is available here.
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Nature Protocols (6)1 2011
Abstract
We developed a method called INTACT (isolation of nuclei tagged in specific cell types) that allows affinity-based isolation of nuclei from individual cell types of a tissue, thereby circumventing the problems associated with mechanical purification techniques. In this method nuclei are affinity-lab...
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PMID: 21212783
PDF is available here.
PDF is available here.
Abstract
We present the method for developing a custom CHO-HCP AMMP assay for bioprocess development. Developing AMMP assays or modifying existing assays into AMMP assays can provide better performance (reproducibility, sensitivity) in complex samples and reduced operator time. The protocol shows the steps f...
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PMID: 21339717
PDF is available here.
PDF is available here.
Abstract
Immunohistochemical techniques detect antigens in tissue sections by means of immunological and chemical reactions. This technique is highly sensitive and specific and can detect a wide variety of antigens in multiple animal species. This chapter reviews common immunohistochemical methods used in th...
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PMID: 20972748
PDF is available here.
PDF is available here.
Abstract
We describe the use of one method commonly used to generate transcriptomics data, namely the use of Affymetrix GeneChip microarrays. Data generated in transcriptomics experiments can be analysed using a multitude of approaches, but a common goal is to identify those transcripts whose abundance is al...
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PMID: 20972753
PDF is available here.
PDF is available here.
Brain research (1360) 2010
Abstract
The inability of axons in central nervous system (CNS) to regenerate after injury is related partly to multiple endogenous axon growth inhibitors including Nogo receptor (NgR). This study tested the hypothesis that silencing NgR expression by adenovirus-mediated RNA interference (RNAi) (AD-NgR) may...
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PMID: 20828545
PDF is available here.
PDF is available here.
Chemical Communications (46)40 2010
Abstract
Luminol functionalized gold nanoparticles were used as labels for electrochemiluminescence signal amplification and an ultrasensitive, highly selective, convenient, low cost DNA detection strategy was developed.
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PMID: 20852765
PDF is available here.
PDF is available here.
Arerugi = [Allergy] (59)11 2010
Abstract
Abstract
We have revealed, for the first time, that the slight difference between the local structures of amino acid residues at the middle sites, SER45 and THR35 for streptavidin and avidin, respectively, strongly affects the microscopic reaction processes, i.e., the variation governs the type of bond as we...
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PMID: 20725689
PDF is available here.
PDF is available here.
Chemical Communications (46)38 2010
Abstract
A small glutamine donor substrate was designed for single-step and site-specific protein multifunctionalization catalyzed by microbial transglutaminase.
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PMID: 20820527
PDF is available here.
PDF is available here.
Abstract
We have developed an immunomicroarray for systematic studies of the binding properties of 10 different micro-/nano-sized streptavidin-functionalized beads to a biotin substrate immobilized on SiO(2) with or without surface modification. SiO(2) surface cleaning, immobilized substrate concentration an...
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PMID: 20417734
PDF is available here.
PDF is available here.
Optics Express (18)20 2010
Abstract
We demonstrate label-free optical biosensing using a horizontal air-slotted silicon-rich silicon nitride (SiNx) microdisk resonator. Due to the strong confinement of light in the air-slot, a large resonance shift of 6.2 nm is observed upon reaction of the biotin-functionalized disk with a streptavid...
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PMID: 20940958
PDF is available here.
PDF is available here.
Journal of Chromatography A (1217)44 2010
Abstract
The use of liposomes as coating materials in capillary electrophoresis has recently emerged as an important and popular research area. There are three preparation methods that are commonly used for coating capillaries with liposomes, namely physical adsorption, avidin-biotin binding and covalent cou...
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PMID: 20870242
PDF is available here.
PDF is available here.
Abstract
We describe the synthesis of the first Thalidomide-biotin analogue in order to initiate investigations into the unknown molecular mode of action of Thalidomide. In this manner we describe the attachment of biotin tether through the Huisgen 1,3-dipolar cycloaddition or "click" synthetic methodology....
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PMID: 20625607
PDF is available here.
PDF is available here.
Lab On a Chip (10)18 2010
Abstract
We report an approach utilizing a suspended nanoparticle crystal (S-NPC) as an electrical read-out biosensor based on a nanofluidic electrokinetics principle. As a preliminary demonstration, streptavidin-modified S-NPC with a particle diameter of 520 nm was used to detect biotin in a PBS buffer. The...
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PMID: 20544114
PDF is available here.
PDF is available here.
Abstract
The R2 for survival curves ranged from 0.93 to 0.99 during the first 10 weeks after infusion of erythrocytes. Erythrocyte survival time for the 3 non-Greyhound dogs was 94, 98, and 116 days, respectively, which was consistent with previously reported values. Erythrocyte survival time for the 6 Greyh...
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PMID: 20807142
PDF is available here.
PDF is available here.
Abstract
DNA piezoelectric biosensors have become a promising tool in molecular medicine since they do not require any label or staining. Here, a DNA piezoelectric biosensor based on a quartz crystal microbalance (QCM) was created to identify abnormal genes causing α-thalassemia 1 (SEA deletion).
The functi...
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PMID: 20578968
PDF is available here.
PDF is available here.
Abstract
In this study, an interface embedded in situ gold nanoparticles (AuNPs) and biotin in chitosan hydrogel was constructed by one-step electrochemical deposition in solution containing tetrachloroauric (III) acid, biotin and chitosan. This deposited interface acts as biosensing platform...
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PMID: 21133092
PDF is available here.
PDF is available here.
Chemical Communications (46)31 2010
Biocompatible post-polymerization functionalization of a water soluble poly(p-phenylene ethynylene).
Abstract
A biocompatible post-polymerization functionalization reaction takes advantage of a polymer's structural motif for the controllable attachment of biotin as a model biosensor that responds to streptavidin.
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PMID: 20596563
PDF is available here.
PDF is available here.
Abstract
Wild type T4 bacteriophage and recombinant T4 bacteriophages displaying biotin binding peptide (BCCP) and cellulose binding module (CBM) on their heads were immobilized on nano-aluminum fiber-based filter (Disruptor), streptavidin magnetic beads and microcrystalline cellulose, respectively. Infectiv...
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PMID: 20561957
PDF is available here.
PDF is available here.
Abstract
A biotin derivative, namely biotin-aminocaproic acid-lysine (BAL), was synthesized with solid-phase chemistry, conjugated to a carrier-protein, and used for rabbit immunization. The aminocaproic acid-lysine "long-arm" was used in order to project the biotin-hapten above the carrier-protein surface....
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PMID: 19950001
PDF is available here.
PDF is available here.
Chemical Communications (46)28 2010
Abstract
Stepwise introduction of multiple streptavidin tetramers into a nanoarray formed in a stick-like punched DNA origami as well as selective removal of predetermined tetramer from the array were successfully achieved by applying a programmed strand displacement technique to a DNA origami scaffold.
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PMID: 20532403
PDF is available here.
PDF is available here.
Abstract
To design efficient targeting strategies in magnetic resonance (MR) molecular imaging applications, the formation of supramolecular adducts between (strept)avidin ((S)Av) and tribiotinylated Gd-DOTA-monoamide complexes (DOTA=1,4,7,10-tetraazacyclododecane-N,N',N'',N'''-tetraacetic acid) was explored...
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PMID: 20533461
PDF is available here.
PDF is available here.
Journal of Comparative Neurology (518)14 2010
Prefrontal cortical inputs to the basal amygdala undergo pruning during late adolescence in the rat.
Abstract
We provide the first evidence that afferents of the basal amygdala (BA) undergo significant structural remodeling during adolescence. We used quantitative tract-tracing and gene expression profiling methods to characterize changes in the medial prefrontal cortical (mPFC) inputs to the BA across ages...
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PMID: 20506471
PDF is available here.
PDF is available here.
Abstract
We demonstrate the formation of nano-thin, poly(ethylene glycol) (PEG)-rich functional conformal coatings on individual islets via layer-by-layer assembly technique. The surface of the islets is modified with biotin-PEG-N-hydroxysuccinimide (NHS), and the islets are further covered by streptavidin (...
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PMID: 20163204
PDF is available here.
PDF is available here.
Nanotechnology (21)25 2010
Abstract
We introduce a method for the acquisition of single molecule force measurements of ligand-receptor interactions using the photonic force microscope (PFM). Biotin-functionalized beads, manipulated with an optical trap, and a streptavidin-functionalized coverslip were used to measure the effect of dif...
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PMID: 20516583
PDF is available here.
PDF is available here.
Abstract
The effects of orally administered biotin supplementation on the growth of claw horn in young, clinically healthy cattle were analyzed. Twelve, 1-year-old Girolando cattle were randomly assigned to receive either 12.5 mg of diluted powdered biotin (GI) or a control treatment (GII) for 40 consecutive...
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PMID: 20808571
PDF is available here.
PDF is available here.
Abstract
The purpose of this study was to assess two-step in vivo tumor targeting by specific biotin-conjugated antibodies and ultrasmall superparamagnetic iron oxide (USPIO)-anti-biotin nanoparticles as contrast agents for magnetic resonance imaging (MRI) at 1.5 T.
D430B huma...
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PMID: 19806404
PDF is available here.
PDF is available here.
Angiogenesis (13)2 2010
Abstract
During the early stages of angiogenesis, following stimulation of endothelial cells by vascular endothelial growth factor (VEGF), the vascular wall is breached, allowing high molecular weight proteins to leak from the vessels to the interstitial space. This hallmark of angiogenesis results in deposi...
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PMID: 20512410
PDF is available here.
PDF is available here.
Anticancer Research (30)6 2010
Abstract
We recently reported that membrane-type 1 matrix metalloproteinase (MT1-MMP) is phosphorylated on its unique cytoplasmic tyrosine residue but the contribution of this event to tumor progression remains unclear. In this work, we show that the non phosphorylizable cell-permeable peptide antennapedia-c...
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PMID: 20651331
PDF is available here.
PDF is available here.
Abstract
We conclude that the PILP are promising gene delivery systems which may be used to target the liver cancer.
Copyright 2010 Elsevier B.V. All rights reserved....
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PMID: 20144670
PDF is available here.
PDF is available here.
Abstract
An ultrasensitive assay for electrical biosensing of syphilis DNA was proposed by using target-guided formation of polyaniline (PANI) based on an enzymatically catalyzed method. The sensitive biodetection relies on the DNA hybridization and biotin-streptavidin interaction. After coupling of biotinyl...
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PMID: 20207129
PDF is available here.
PDF is available here.
Abstract
We report on a rapid method for DNA hybridisation and isolation within a microfluidic device, where all reaction and washing steps are performed in continuous flow in an automated fashion within less than two minutes. Magnetic particles were used as a solid support and manipulated through laminar fl...
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PMID: 20181469
PDF is available here.
PDF is available here.
Abstract
We report a mass spectrometry-based strategy for quantitative analysis of cell surface membrane proteome changes. The strategy includes enrichment of surface membrane proteins using a membrane-impermeable chemical probe followed by stable isotope (18)O labeling and LC-MS analysis. We applied this st...
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PMID: 20380418
PDF is available here.
PDF is available here.
Abstract
In the quest of obtaining a molecular probe for in situ detection of pectin methylesterase (PME), the PME inhibitor (PMEI) was biotinylated and the biotinylated PMEI (bPMEI) was extensively characterized. Reaction conditions for single labeling of the purified PMEI with retention of its inhibitory c...
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PMID: 20380375
PDF is available here.
PDF is available here.
Abstract
Biotinidase deficiency is a biotin-responsive, inherited neurocutaneous disorder. The disorder is readily treatable and is screened for in the newborn period. Over the years since the discovery of the disorder, many practical questions and issues have been raised as to the diagnosis, management, tre...
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PMID: 20129807
PDF is available here.
PDF is available here.
Chemical Communications (46)16 2010
Abstract
We report upon the application of quantum dot barcodes prepared by layer-by-layer biological self-assembly of quantum dot-biotin and quantum dot-streptavidin conjugates on magnetic beads for qualitative multiplexed immunoassay.
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PMID: 20369192
PDF is available here.
PDF is available here.
Abstract
Fluorophore-labeled bioprobes are the key for fluorescent-labeled imaging technology. In the present work, mouse liver hepatoma cell line BNL 1ME A.7R.1 (MEAR)-specific ssDNA aptamer TLS9a was used to fabricate quantum dot-labeled aptamer bioprobe (QD-Apt), which was obtained by conjugating streptav...
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PMID: 20188954
PDF is available here.
PDF is available here.
Bioconjugate Chemistry (21)4 2010
Abstract
We reported emulsion polymerization of propylene sulfide with Pluronic F127 as an emulsifier, yielding nanoparticles (NPs) in the 25 nm size range. Immunologically functional NPs were prepared by adding an antigen-Pluronic conjugate to the polymerization mixture ( Reddy , S. T. , et al. ( 2007 ) Nat...
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PMID: 20369815
PDF is available here.
PDF is available here.
Methods in Molecular Biology (639)639 2010
Abstract
We applied the Arabidopsis Affymetrix tiling arrays to study the whole-genome transcriptome under drought, cold, and high-salinity stresses and identified a large number of drought, cold, and high-salinity stress-inducible genes and transcriptional units (TUs)....
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PMID: 20387044
PDF is available here.
PDF is available here.
Abstract
We have developed the first example of a one-donor/three-acceptor multicolor time-resolved fluorescence energy transfer (TR-FRET) system, and we have exemplified its use by monitoring a ligand-regulated protein-protein exchange process in a four-component biological system. By careful quantification...
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PMID: 20230029
PDF is available here.
PDF is available here.
Abstract
We demonstrate protein-binding-induced disassembly of dendrimer-based amphiphilic nanocontainers. We show that this disassembly is selective to the targeted protein and that the disassembly event causes a release of the sequestered guest molecules. We propose that the disassembly is caused by altera...
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PMID: 20232866
PDF is available here.
PDF is available here.
Abstract
The "cholinergic hypothesis" of dementia posits that the progressive loss of basal forebrain cholinergic neurons and the consequent decrease of acetylcholine levels in the deafferented projection sites are correlated with degree of cognitive decline in dementia. It has also been proposed that early...
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PMID: 20164586
PDF is available here.
PDF is available here.
Abstract
We analyzed the ability of non-covalently linked PTDs to internalize the model binding proteins streptavidin (SA) and Strep-Tactin (ST). Notably, inside the cell, the PTD-Strep-tag II ligand used for internalization of SA was displaced by the model target biotin which exhibits a higher binding affin...
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PMID: 20198304
PDF is available here.
PDF is available here.
Chemical Communications (46)13 2010
Abstract
A new method for site-specific caging of plasmid DNA was developed to enable light-activation of gene expression in living cells by exposure to a low dose of light.
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PMID: 20234920
PDF is available here.
PDF is available here.
Thrombosis Research (125 Suppl 2) 2010
Abstract
Cancer-associated thrombosis is a major cause of morbidity and mortality. Although anticoagulation remains the mainstay for prevention and treatment of thrombosis, current anticoagulants are problematic in cancer patients. Parenteral anticoagulants, such as heparin or low-molecular heparin, require...
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PMID: 20434001
PDF is available here.
PDF is available here.
Amino Acids (38)4 2010
Abstract
Granulocyte-colony stimulating factor stimulates production and antibacterial function of neutrophiles. Therapy using the recombinant protein drug represents a major step forward in oncology. The protein has not been, however, completely sequenced at the protein level and this formed the rationale o...
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PMID: 19526310
PDF is available here.
PDF is available here.
Abstract
A sensitive biotin-streptavidin amplified enzyme-linked immunosorbent assay (BA-ELISA) method was developed for the determination of chloramphenicol residues in milk. The biotin-streptavidin system was applied to enhance the sensitivity. After optimization, the detection limit of the method was foun...
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PMID: 20192212
PDF is available here.
PDF is available here.
Abstract
For being fully efficient a targeted delivery system should associate simultaneously multiple functionalities. In this context, the association of several polymeric materials to form composite multifunctional particles can be foreseen. The present work describes the synthesis of different derivates...
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PMID: 20005933
PDF is available here.
PDF is available here.
Methods in Molecular Biology (624)624 2010
Abstract
We will also discuss FSNP surface modification and bioconjugation techniques that are useful for cancer cell imaging....
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PMID: 20217594
PDF is available here.
PDF is available here.
Methods in Molecular Biology (587)587 2010
Abstract
We developed a high-spatial resolution optical-trapping assay for RecBCD. The RecBCD is anchored to the surface via a genetically engineered biotin. This RecBCD-bio exhibited native activity, as measured by biochemical assays. Motion is continuous down to a detection limit of 2 nm, implying a unitar...
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PMID: 20225148
PDF is available here.
PDF is available here.
Methods in Molecular Biology (627)627 2010
Abstract
Qualitative and quantitative aspects of protein interactions with membranes may be studied by optical sensors. Biacore offers two dedicated chips for working with lipids and membranes: the L1 and HPA sensor chips. The L1 chip is the most frequently used in protein-membrane interaction studies and it...
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PMID: 20217623
PDF is available here.
PDF is available here.
Methods in Molecular Biology (627)627 2010
Abstract
Surface analytical tools as surface plasmon resonance (SPR) have become increasingly important in biomedical research since they offer high detection sensitivity compared to traditional biomedical methods. For the use of SPR as a biomedical research tool there is a need to immobilize the reactants t...
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PMID: 20217626
PDF is available here.
PDF is available here.
Abstract
Based on the application of our recent biotinyl photoprobe with a cleavable N-acylsulfonamide, an efficient process has been developed for profiling photoaffinity labeled peptides among a large excess of unlabeled concomitants. N-acylsulfonamide group was found to be stable under the usual S-pyridyl...
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PMID: 20185307
PDF is available here.
PDF is available here.
Abstract
We report a global and quantitative analysis of staphylococcal cell surface-associated proteins using a combination of (14)N(15)N metabolic labeling, biotinylation, and GeLC-MS/MS. To address the important question of S. aureus pathophysiology, we analyzed the influence of the alternative sigma fact...
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PMID: 20108986
PDF is available here.
PDF is available here.
Acta Biomaterialia (6)3 2010
Abstract
Silicon-doped hydroxyapatite has been functionalized with biotin molecules as a new methodology for the attachment of proteins, peptides or growth factors through the formation of avidin-biotin complex in this material. Bioceramic biotinylation has been performed by esterification re...
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PMID: 19751850
PDF is available here.
PDF is available here.
Abstract
We report the selection of a group of aptamer candidates (85 mer) against streptavidin. Through comparing the predicted secondary structures of all the candidates, a conservative bulge-hairpin structure section (about 29 mer) was found, and then it was determined to be the binding motif to streptavi...
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PMID: 20153201
PDF is available here.
PDF is available here.
Journal of Biotechnology (145)4 2010
Abstract
We describe an efficient system for one-step purification and immobilization of recombinant proteins using tamavidin 2 as an affinity tag. A bacterial sialyltransferase and soybean agglutinin were fused to tamavidin 2 and expressed in E. coli and tobacco BY-2 cells, respectively. High-level expressi...
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PMID: 20026208
PDF is available here.
PDF is available here.
Brain research (1313) 2010
Abstract
Application of different fluorescent tracers to the right and left hypoglossal nerve of the frog revealed the extent of dendrites crossing the midline into the territory of contralateral hypoglossal motoneurons. By using confocal microscopy, a large number of close appositions were detected between...
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PMID: 19962369
PDF is available here.
PDF is available here.
Abstract
Whereas older literature has focused on the relationship among nutritional deficiency, malnutrition and poverty, recent research has identified additional patient populations that are at risk for developing nutritional deficiencies. These populations include premature infants, patients with long-ter...
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PMID: 19966568
PDF is available here.
PDF is available here.