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Antibody Specificity (2):
Recent article
Antibody Specificity
Abstract
We used x-ray crystallography and 454 pyrosequencing to characterize additional VRC01-like antibodies from HIV-1-infected individuals. Crystal structures revealed a convergent mode of binding for diverse antibodies to the same CD4-binding-site epitope. A functional genomics analysis of expressed hea...
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PMID: 21835983
PDF is available here.
PDF is available here.
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Abstract
Abstract
Abstract
We generated two novel mouse anti-human GL50 monoclonal antibodies (MAbs) using classical hybridoma technology. The two MAbs (clones 2B4 and 4D11) were IgG1 (κ) and IgG2a (κ), respectively, and bound specifically to human GL50. Epitope competition assay showed that 2B4 and 4D11 bind to the same ep...
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PMID: 21851236
PDF is available here.
PDF is available here.
Abstract
We cloned 576 new HIV antibodies from four unrelated individuals. All four individuals produced expanded clones of potent broadly neutralizing CD4-binding-site antibodies that mimic binding to CD4. Despite extensive hypermutation, the new antibodies shared a consensus sequence of 68 immunoglobulin H...
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PMID: 21764753
PDF is available here.
PDF is available here.
Xenobiotica (41)5 2011
Abstract
We describe a targeted approach for affinity improvement of therapeutic antibodies. Sets of CDR residues that are solvent accessible and relatively diverse in natural antibodies are targeted for diversification. Degenerate oligonucleotides are used to generate combinatorial phage-displayed antibody...
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PMID: 19252851
PDF is available here.
PDF is available here.
Xenobiotica (41)5 2011
Abstract
Monoclonal antibodies (mabs) are powerful tools for the quantification, detection, and targeting of specific molecules. Allergen-specific mabs are important for the quantification of major allergens in allergen preparations used for allergen-specific immunotherapy and allergy diagnosis. Indeed, prog...
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PMID: 18612608
PDF is available here.
PDF is available here.
Abstract
BACKGROUND.: The relationship between humoral rejection and human leukocyte antigen (HLA) antibodies is established. Proteinuria is the hallmark of glomerular injury. The relationship between HLA antibody and proteinuria was explored in renal transplant recipients developing de novo...
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PMID: 21519315
PDF is available here.
PDF is available here.
Abstract
These results indicated that the described competitive ELISA methods were sensitive and valuable tools for quantifying vitellogenin in rare minnow plasma. These methods were then applied to measure R-VTG concentrations in plasma of male fish exposed to a series of E(2) concentrations. When E(2) leve...
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PMID: 21477841
PDF is available here.
PDF is available here.
Xi bao yu fen zi mian yi xue za zhi = Chinese journal of cellular and molecular immunology (27)4 2011
Abstract
To produce the monoclonal antibodies (mAb) against Heavy Metal Chromium, and develop a competitive inhibition enzyme-linked immunosorbent assay (ciELISA) for the detection of Chromium.
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PMID: 21481322
PDF is available here.
PDF is available here.
Abstract
To produce anti-19-Nortestosterone (NT) monoclonal antibodies and identify their immunological characteristics.
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PMID: 21565689
PDF is available here.
PDF is available here.
Abstract
A sensitive and broad class selective direct competitive enzyme-linked immunosorbent assay (ELISA) using monoclonal antibody (McAb) has been described for the detection of pyrethroids with phenoxybenzene group. One monoclonal antibody, 2G(2)E(7), was obtained and characterized after fusion of myeloma...
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PMID: 21381771
PDF is available here.
PDF is available here.
Clinical Cancer Research (17)9 2011
Abstract
We investigated the expression of MMSET protein in different types of human tumors....
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PMID: 21385930
PDF is available here.
PDF is available here.
Abstract
We report an effective application of luminescent semiconductive nanoparticles, quantum dots (QDs), as a labeling material of the IgM antibody. The CdSe carboxyl QDs were reacted with 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide hydrochloride and N-hydroxysulfo- succinimide in 2-(morpholino) ethan...
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PMID: 21521661
PDF is available here.
PDF is available here.
Abstract
Inactivated influenza virus vaccine prepared from a non-pathogenic influenza virus strain A/duck/Hokkaido/Vac-1/2004 (H5N1) from the virus library conferred protective immunity to chickens against the challenge of antigenically drifted highly pathogenic avian influenza virus (HPAIV),...
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PMID: 21476487
PDF is available here.
PDF is available here.
Abstract
In order to get a rapid specific diagnostic reagent for subgroup A Avian Leukosis Virus detection.
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PMID: 21465800
PDF is available here.
PDF is available here.
Abstract
We addressed the question in the mouse model how the Th1/Th2 bias of primed immune responses by a DNA vaccine influences hepatitis B virus (HBV) clearance.
Plasmids expressing HBV core protein (HBcAg) or HBV e antigen and HBcAg fused to the extracellular domain of CTLA-4 (pCTLA-4-HBc...
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PMID: 21799884
PDF is available here.
PDF is available here.
Abstract
For efficient prevention of viral infections and cross protection, simultaneous targeting of multiple viral epitopes is a powerful strategy. Llama heavy chain antibody fragments (VHH) against the trimeric envelope proteins of Respiratory Syncytial Virus (Fusion protein), Rabies virus...
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PMID: 21483777
PDF is available here.
PDF is available here.
Abstract
We show that the peptides bind on the surface of these cells and obtained clones that could even specifically bind to only specific regions of cells indicating restricted distribution of the epitopes. What was peculiar and interesting was that the epitopes were carbohydrate in nature. This gives ins...
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PMID: 21364990
PDF is available here.
PDF is available here.
Abstract
We have developed optimized methods that allow the rapid assembly of scFv libraries from chickens immunized with pools of immunogens. These methods allow the simplified generation of a single moderately sized library of single-chain Fv (scFv) and the subsequent isolation of diverse, high-affinity, a...
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PMID: 20978977
PDF is available here.
PDF is available here.
Abstract
Redox-dependent thylakoid protein phosphorylation regulates both the short- and long-term acclimation of the photosynthetic apparatus to changes in environmental conditions. The major thylakoid phosphoproteins belong to photosystem II (D1, D2, CP43, PsbH) and its light-harvesting antenna (Lhcb1, Lhc...
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PMID: 20960130
PDF is available here.
PDF is available here.
Abstract
We discuss the inherent limitations of classical polyclonal and monoclonal antibody generation and highlight an attractive alternative: generating high specificity, high affinity recombinant antibodies from alternative immune sources such as chickens, via phage display....
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PMID: 20978962
PDF is available here.
PDF is available here.
Abstract
We critically consider the mechanistic bases for this specificity/degeneracy enigma and also compare and contrast Ag recognition by Abs and TCRs....
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PMID: 21173256
PDF is available here.
PDF is available here.
Abstract
We used new domain-specific antibodies and small interfering RNA technology to uncover physiological functions of kindlin-2 in human dermal fibroblasts in vitro and in adult skin in situ. In primary dermal fibroblasts, kindlin-2 is essential for the integrin clustering and activation, and it regulat...
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PMID: 20861856
PDF is available here.
PDF is available here.
Abstract
We present a novel analysis of membrane-protein expression in tissue sections based on semi-automatic real-time measurement using LigandTracer(®) technology. A commercial antiHER2 antibody developed for immunohistochemistry used in this setup was revealed to have sub-optimal interaction with tissue...
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PMID: 20828661
PDF is available here.
PDF is available here.
Abstract
Abstract
We describe a novel series of 10 antibodies towards GPA, recognizing four extra- and intracellular peptide epitopes of this molecule (defined by epitope mapping) and one mixed peptide/carbohydrate epitope. All antibodies bind better to the desialylated than to the fully sialylated molecule, includin...
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PMID: 20727998
PDF is available here.
PDF is available here.
Abstract
Recent findings have brought optimism that development of a successful human immunodeficiency virus type-1 (HIV-1) vaccine lies within reach. Studies of early events in HIV-1 infection have revealed when and where HIV-1 is potentially vulnerable to vaccine-targeted immune responses. With technical a...
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PMID: 21029964
PDF is available here.
PDF is available here.
Abstract
During a severe outbreak of bacterial heart rot that occurred in pineapple plantations on Oahu, Hawaii, in 2003 and years following, 43 bacterial strains were isolated from diseased plants or irrigation water and identified as Erwinia chrysanthemi (now Dickeya sp.) by phenotypic, mol...
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PMID: 21050038
PDF is available here.
PDF is available here.
Abstract
We developed an enzyme-linked immunosorbent assay (ELISA) for bovine SP-D and determined the concentration of SP-D in bronchoalveolar lavage fluid (BALF) from calves. Bovine SP-D was purified from BALF using a mannose-Shepharose 6B column. The obtained 44 kDa protein was identified as bovine SP-D by...
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PMID: 20526045
PDF is available here.
PDF is available here.
Autoimmunity Reviews (9)11 2010
Abstract
A novel approach for the selective silencing of targeted autoreactive B lymphocytes is reviewed that mimics the physiological mechanisms for suppressing B cell activity. It is based on the use of bi- or tri-specific chimeric antibodies that cross-link BCRs with a pre-selected antigen...
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PMID: 20601201
PDF is available here.
PDF is available here.
Abstract
The Shiga-like toxins (Stx) are critical virulence factors of enterohaemorrhagic Escherichia coli (EHEC). Stx1B subunit plays important roles in EHEC infection. This work aims to generate and characterize monoclonal antibodies (mAbs) against the Stx1B and to investigate their utility in discriminati...
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PMID: 20854398
PDF is available here.
PDF is available here.
Abstract
We expressed a truncated gene encoding the N terminal half of TGEV S gene (designated S1 gene) in a prokaryotic system. The resulting S1 protein was used to immunize BALB/c mice followed by the generation of a monoclonal antibody (MAb). A generated MAb (7F9) was identified by ELISA and the chromosom...
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PMID: 20715993
PDF is available here.
PDF is available here.
Abstract
We have for the first time generated a monoclonal antibody against DARPP-32. Enzyme-linked immunosorbent assay (ELISA) and Western immunoblotting were used to screen the hybridomas. As a result, one MAb named Y8 (IgG1) was characterized, which bound to the native DARPP-32 protein. Then the MAb was u...
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PMID: 20715994
PDF is available here.
PDF is available here.
Zhong nan da xue xue bao. Yi xue ban = Journal of Central South University. Medical sciences (35)8 2010
Abstract
To compare the in vitro antitumor immune responses induced by bivalent bispecific anti-idiotype antibody G22-I50 and monovalent anti-idiotype antibody G22 and I50, and explore its possible mechanism.
Proteins G22-I50, G22, and I50 were induced and identified by Wester...
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PMID: 20818068
PDF is available here.
PDF is available here.
Abstract
We have generated a panel of novel monoclonal antibodies (MAbs) against VLY. For the generation of MAbs, we have used recombinant VLY expressed in Escherichia coli. The functional activity of recombinant VLY was confirmed by an in vitro hemolytic assay using human erythrocytes. The MAbs raised again...
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PMID: 20298711
PDF is available here.
PDF is available here.
Xi bao yu fen zi mian yi xue za zhi = Chinese journal of cellular and molecular immunology (26)8 2010
Abstract
We studied the cellular localization of HPPCn. The mAb epitope was also analyzed using peptide phage display technology.
An anti-HPPCn mAb, named W2-D5, was obtained. It belongs to IgG1 subclass. It could specially bind to human HPPCn. Furthermore, by immunofluorescence results, wo confirmed HPPCn l...
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PMID: 20619106
PDF is available here.
PDF is available here.
Abstract
We sought to evaluate the association between early-life pet exposure and allergic sensitization at age 18 years.
Teenagers who had been enrolled in the Detroit Childhood Allergy Study birth cohort in 1987-1989 were contacted at age 18 years. Serum total and allergen-specific IgE levels to 7 common...
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PMID: 20579718
PDF is available here.
PDF is available here.
Journal of Immunological Methods (359)1-2 2010
Abstract
We show that antibody-expressing clones can be simultaneously cultured and analysed rapidly in antigen-coated ELISA plate wells yielding high binding signals and saving valuable selection time, while also eliminating the necessity for antibody extraction. The utilisation of the 'on-plate' technique...
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PMID: 20493872
PDF is available here.
PDF is available here.
Abstract
A neutralizing epitope fragment of ApxIIA toxin (ApxIIA#5) of the Korean Actinobacillus pleuropneumoniae serotype 2 strain was expressed and immobilized on the cell surface of Saccharomyces cerevisiae for efficient vaccine development. Expression of ApxIIA#5 was confirmed by Western blot analysis us...
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PMID: 20622458
PDF is available here.
PDF is available here.
Abstract
We used knowledge of HIV-1 envelope (Env) structure to develop antigenically resurfaced glycoproteins specific for the structurally conserved site of CD4 receptor binding. These probes were used to identify sera with NAbs to the CD4-binding site (CD4bs) and to isolate individual B cells from such an...
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PMID: 20616233
PDF is available here.
PDF is available here.
Zhonghua gan zang bing za zhi = Zhonghua ganzangbing zazhi = Chinese journal of hepatology (18)7 2010
Abstract
Screening monoclonal antibodies selectively distribute on hepatocellular membrane by hybridoma technology and exploring their relationship with liver diseases.
Plasma membrane vesicles of rat hepatocytes were prepared using density gradient centrifugation and BALB/c mice were immunized with the memb...
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PMID: 20678446
PDF is available here.
PDF is available here.
Clinical Cancer Research (16)13 2010
Abstract
We used immunohistochemistry to examine recently developed antibodies specific to major hotspot mutations of L858R and DEL E746-A750.
We used five series of lung cancers: 47 non-small cell lung cancers (NSCLC) to evaluate various types of EGFR mutations, a consecutive series of 238 N...
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PMID: 20570926
PDF is available here.
PDF is available here.
Abstract
To obtain the soluble single-chain fragment V (ScFv) monoclonal antibodies (McAbs) against the SSA antigen epitopes.
Three octapeptides (60000 SSA antigen residues 482-493 termed as P1 epitope, residues 310-323 termed as P2 epitope and residues 230-241 termed as P3 epitope) were synthesized on the l...
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PMID: 20979776
PDF is available here.
PDF is available here.
Abstract
Studies of gene regulated by estrogen in breast cancer 1 (GREB1) have focused on mRNA levels with limited evidence about GREB1 protein expression in normal and breast cancer cells. A monoclonal antibody that recognizes GREB1 protein in breast tissues could be applied to correlate pro...
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PMID: 19842031
PDF is available here.
PDF is available here.
Cancer Research (70)13 2010
Abstract
We showed that E2 is expressed at various precursor stages of cervical carcinoma by immunohistochemistry on paraffin-embedded clinical samples. E2 was found to be highly expressed in the nuclei and cytoplasm of cells forming the intermediate and upper layers of cervical intraepithelial neoplasia (CI...
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PMID: 20530671
PDF is available here.
PDF is available here.
Cancer Research (70)13 2010
Abstract
We showed that E2 is expressed at various precursor stages of cervical carcinoma by immunohistochemistry on paraffin-embedded clinical samples. E2 was found to be highly expressed in the nuclei and cytoplasm of cells forming the intermediate and upper layers of cervical intraepithelial neoplasia (CI...
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PMID: 20530671
PDF is available here.
PDF is available here.
Cancer Research (70)13 2010
Abstract
We showed that E2 is expressed at various precursor stages of cervical carcinoma by immunohistochemistry on paraffin-embedded clinical samples. E2 was found to be highly expressed in the nuclei and cytoplasm of cells forming the intermediate and upper layers of cervical intraepithelial neoplasia (CI...
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PMID: 20530671
PDF is available here.
PDF is available here.
Cancer Research (70)13 2010
Abstract
We showed that E2 is expressed at various precursor stages of cervical carcinoma by immunohistochemistry on paraffin-embedded clinical samples. E2 was found to be highly expressed in the nuclei and cytoplasm of cells forming the intermediate and upper layers of cervical intraepithelial neoplasia (CI...
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PMID: 20530671
PDF is available here.
PDF is available here.
Cancer Research (70)13 2010
Abstract
We showed that E2 is expressed at various precursor stages of cervical carcinoma by immunohistochemistry on paraffin-embedded clinical samples. E2 was found to be highly expressed in the nuclei and cytoplasm of cells forming the intermediate and upper layers of cervical intraepithelial neoplasia (CI...
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PMID: 20530671
PDF is available here.
PDF is available here.
Cancer Research (70)13 2010
Abstract
We showed that E2 is expressed at various precursor stages of cervical carcinoma by immunohistochemistry on paraffin-embedded clinical samples. E2 was found to be highly expressed in the nuclei and cytoplasm of cells forming the intermediate and upper layers of cervical intraepithelial neoplasia (CI...
|
PMID: 20530671
PDF is available here.
PDF is available here.
Cancer Research (70)13 2010
Abstract
We showed that E2 is expressed at various precursor stages of cervical carcinoma by immunohistochemistry on paraffin-embedded clinical samples. E2 was found to be highly expressed in the nuclei and cytoplasm of cells forming the intermediate and upper layers of cervical intraepithelial neoplasia (CI...
|
PMID: 20530671
PDF is available here.
PDF is available here.
Cancer Research (70)13 2010
Abstract
We showed that E2 is expressed at various precursor stages of cervical carcinoma by immunohistochemistry on paraffin-embedded clinical samples. E2 was found to be highly expressed in the nuclei and cytoplasm of cells forming the intermediate and upper layers of cervical intraepithelial neoplasia (CI...
|
PMID: 20530671
PDF is available here.
PDF is available here.
Cancer Research (70)13 2010
Abstract
We showed that E2 is expressed at various precursor stages of cervical carcinoma by immunohistochemistry on paraffin-embedded clinical samples. E2 was found to be highly expressed in the nuclei and cytoplasm of cells forming the intermediate and upper layers of cervical intraepithelial neoplasia (CI...
|
PMID: 20530671
PDF is available here.
PDF is available here.
Cancer Research (70)13 2010
Abstract
We showed that E2 is expressed at various precursor stages of cervical carcinoma by immunohistochemistry on paraffin-embedded clinical samples. E2 was found to be highly expressed in the nuclei and cytoplasm of cells forming the intermediate and upper layers of cervical intraepithelial neoplasia (CI...
|
PMID: 20530671
PDF is available here.
PDF is available here.
Cancer Research (70)13 2010
Abstract
We showed that E2 is expressed at various precursor stages of cervical carcinoma by immunohistochemistry on paraffin-embedded clinical samples. E2 was found to be highly expressed in the nuclei and cytoplasm of cells forming the intermediate and upper layers of cervical intraepithelial neoplasia (CI...
|
PMID: 20530671
PDF is available here.
PDF is available here.
Cancer Research (70)13 2010
Abstract
We showed that E2 is expressed at various precursor stages of cervical carcinoma by immunohistochemistry on paraffin-embedded clinical samples. E2 was found to be highly expressed in the nuclei and cytoplasm of cells forming the intermediate and upper layers of cervical intraepithelial neoplasia (CI...
|
PMID: 20530671
PDF is available here.
PDF is available here.
Cancer Research (70)13 2010
Abstract
We showed that E2 is expressed at various precursor stages of cervical carcinoma by immunohistochemistry on paraffin-embedded clinical samples. E2 was found to be highly expressed in the nuclei and cytoplasm of cells forming the intermediate and upper layers of cervical intraepithelial neoplasia (CI...
|
PMID: 20530671
PDF is available here.
PDF is available here.
Cancer Research (70)13 2010
Abstract
We showed that E2 is expressed at various precursor stages of cervical carcinoma by immunohistochemistry on paraffin-embedded clinical samples. E2 was found to be highly expressed in the nuclei and cytoplasm of cells forming the intermediate and upper layers of cervical intraepithelial neoplasia (CI...
|
PMID: 20530671
PDF is available here.
PDF is available here.
Cancer Research (70)13 2010
Abstract
We showed that E2 is expressed at various precursor stages of cervical carcinoma by immunohistochemistry on paraffin-embedded clinical samples. E2 was found to be highly expressed in the nuclei and cytoplasm of cells forming the intermediate and upper layers of cervical intraepithelial neoplasia (CI...
|
PMID: 20530671
PDF is available here.
PDF is available here.