Abstract
We demonstrate a new protein detection methodology based upon frequency domain electrical measurement using silicon nanowire field-effect transistor (SiNW FET) biosensors. The power spectral density of voltage from a current-biased SiNW FET shows 1/f-dependence in frequency domain for measurements o...
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PMID: 20698634
PDF is available here.
Abstract
A new strategy was described for amplifying the response of the antigen-antibody sensing processes by functionalizing SiO(2) nanoparticles labeled secondary antibodies based on a sandwich immunoassay in this work. At first, the multi-walled carbon nanotubes (CNTs) were individually dispersed in the...
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PMID: 20523934
PDF is available here.
Abstract
These results demonstrate the high sensitivity achieved with this immunosensor design which compares favourably with other previous reports. The immunosensor also exhibited a high selectivity with respect to other corticosteroid compounds closely related to cortisol. The usefulness of the immunosens...
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PMID: 20577675
PDF is available here.
Abstract
We have setup and validated a T cell activation assay to test antigen expression on patient-derived tumor tissues. Chimeric antibody-based receptor (CAR) directed against CAIX, currently used in a clinical trial to treat RCC patients, was used as a model receptor. Primary human T cells expressing CA...
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PMID: 20460126
PDF is available here.
Abstract
Flow cytometry operators often apply familiar white blood cell (WBC) methods when studying red blood cell (RBC) antigens and antibodies. Some WBC methods are not appropriate for RBCs, as the analysis of RBCs requires special considerations, for example, avoidance of agglutination. One hundred sevent...
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PMID: 20656186
PDF is available here.
Abstract
We have used surface plasmon resonance (SPR) to dissect mechanism of pore formation. The membrane association of PFN was calcium dependent irrespective of pH. However, PFN does not permeabilize large or giant unilamellar vesicles (GUV) at pH 5.5 even though the monomers bind to the membranes in the...
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PMID: 20580434
PDF is available here.
Abstract
Thus, p-ANCAs in autoimmune liver diseases are directed against human TBB-5 cross-reacting with the bacterial protein FtsZ, probably reflecting an abnormal immune response to intestinal microorganisms in susceptible, possibly genetically predisposed individuals....
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PMID: 19951907
PDF is available here.
Abstract
Lupus nephritis (LN) remains the most common severe manifestation of systemic lupus erythematosus (SLE) characterized by the presence of autoantibodies (Abs) that are believed to play a central role in the pathogenesis of LN. Among more than 100 Abs reported in SLE, only a few display a direct glomer...
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PMID: 20414746
PDF is available here.
Abstract
I antibodies (HLA-I Abs) in acute non-hemolytic transfusion reactions (ANHTRs) using solid phase assays (SPAs) and conventional complement-dependent lymphocyte cytotoxicity test (LCT). ANHTRs are major issues in transfusion medicine. Anti-leukocyte antibodies have been implicated as one of the causa...
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PMID: 19883399
PDF is available here.
Abstract
We detail the properties of CD20 that make it such an effective therapeutic target and describe how different mAbs change the membrane distribution and internalization of CD20 and have distinct modes of cytotoxic activity....
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PMID: 20350657
PDF is available here.
Abstract
To assess the ability of an anti-lysozyme antibody to stabilize the plasma pharmacokinetics of lysozyme through a 'buffering' effect.
Hen egg lysozyme was radiolabeled with (14)C and infused with or without the murine anti-lysozyme antibody D1.3 into the jugular vein...
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PMID: 19922461
PDF is available here.
Abstract
Recent reports suggest that maternal immunization against low-frequency, platelet (PLT)-specific glycoprotein (GP) polymorphisms is a more common cause of neonatal alloimmune thrombocytopenia (NATP) than previously thought.
Serologic and molecular studies were perform...
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PMID: 19821948
PDF is available here.
Abstract
Recent reports suggest that maternal immunization against low-frequency, platelet (PLT)-specific glycoprotein (GP) polymorphisms is a more common cause of neonatal alloimmune thrombocytopenia (NATP) than previously thought.
Serologic and molecular studies were perform...
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PMID: 19821948
PDF is available here.
Abstract
Recent reports suggest that maternal immunization against low-frequency, platelet (PLT)-specific glycoprotein (GP) polymorphisms is a more common cause of neonatal alloimmune thrombocytopenia (NATP) than previously thought.
Serologic and molecular studies were perform...
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PMID: 19821948
PDF is available here.
Abstract
Recent reports suggest that maternal immunization against low-frequency, platelet (PLT)-specific glycoprotein (GP) polymorphisms is a more common cause of neonatal alloimmune thrombocytopenia (NATP) than previously thought.
Serologic and molecular studies were perform...
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PMID: 19821948
PDF is available here.
Abstract
Recent reports suggest that maternal immunization against low-frequency, platelet (PLT)-specific glycoprotein (GP) polymorphisms is a more common cause of neonatal alloimmune thrombocytopenia (NATP) than previously thought.
Serologic and molecular studies were perform...
|
PMID: 19821948
PDF is available here.
Abstract
Recent reports suggest that maternal immunization against low-frequency, platelet (PLT)-specific glycoprotein (GP) polymorphisms is a more common cause of neonatal alloimmune thrombocytopenia (NATP) than previously thought.
Serologic and molecular studies were perform...
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PMID: 19821948
PDF is available here.
Abstract
Recent reports suggest that maternal immunization against low-frequency, platelet (PLT)-specific glycoprotein (GP) polymorphisms is a more common cause of neonatal alloimmune thrombocytopenia (NATP) than previously thought.
Serologic and molecular studies were perform...
|
PMID: 19821948
PDF is available here.
Abstract
Recent reports suggest that maternal immunization against low-frequency, platelet (PLT)-specific glycoprotein (GP) polymorphisms is a more common cause of neonatal alloimmune thrombocytopenia (NATP) than previously thought.
Serologic and molecular studies were perform...
|
PMID: 19821948
PDF is available here.
Abstract
Recent reports suggest that maternal immunization against low-frequency, platelet (PLT)-specific glycoprotein (GP) polymorphisms is a more common cause of neonatal alloimmune thrombocytopenia (NATP) than previously thought.
Serologic and molecular studies were perform...
|
PMID: 19821948
PDF is available here.
Abstract
Recent reports suggest that maternal immunization against low-frequency, platelet (PLT)-specific glycoprotein (GP) polymorphisms is a more common cause of neonatal alloimmune thrombocytopenia (NATP) than previously thought.
Serologic and molecular studies were perform...
|
PMID: 19821948
PDF is available here.
Abstract
Recent reports suggest that maternal immunization against low-frequency, platelet (PLT)-specific glycoprotein (GP) polymorphisms is a more common cause of neonatal alloimmune thrombocytopenia (NATP) than previously thought.
Serologic and molecular studies were perform...
|
PMID: 19821948
PDF is available here.
Abstract
Recent reports suggest that maternal immunization against low-frequency, platelet (PLT)-specific glycoprotein (GP) polymorphisms is a more common cause of neonatal alloimmune thrombocytopenia (NATP) than previously thought.
Serologic and molecular studies were perform...
|
PMID: 19821948
PDF is available here.
Abstract
Recent reports suggest that maternal immunization against low-frequency, platelet (PLT)-specific glycoprotein (GP) polymorphisms is a more common cause of neonatal alloimmune thrombocytopenia (NATP) than previously thought.
Serologic and molecular studies were perform...
|
PMID: 19821948
PDF is available here.
Abstract
Recent reports suggest that maternal immunization against low-frequency, platelet (PLT)-specific glycoprotein (GP) polymorphisms is a more common cause of neonatal alloimmune thrombocytopenia (NATP) than previously thought.
Serologic and molecular studies were perform...
|
PMID: 19821948
PDF is available here.
Abstract
Recent reports suggest that maternal immunization against low-frequency, platelet (PLT)-specific glycoprotein (GP) polymorphisms is a more common cause of neonatal alloimmune thrombocytopenia (NATP) than previously thought.
Serologic and molecular studies were perform...
|
PMID: 19821948
PDF is available here.
Abstract
Recent reports suggest that maternal immunization against low-frequency, platelet (PLT)-specific glycoprotein (GP) polymorphisms is a more common cause of neonatal alloimmune thrombocytopenia (NATP) than previously thought.
Serologic and molecular studies were perform...
|
PMID: 19821948
PDF is available here.
Abstract
Recent reports suggest that maternal immunization against low-frequency, platelet (PLT)-specific glycoprotein (GP) polymorphisms is a more common cause of neonatal alloimmune thrombocytopenia (NATP) than previously thought.
Serologic and molecular studies were perform...
|
PMID: 19821948
PDF is available here.
Abstract
Recent reports suggest that maternal immunization against low-frequency, platelet (PLT)-specific glycoprotein (GP) polymorphisms is a more common cause of neonatal alloimmune thrombocytopenia (NATP) than previously thought.
Serologic and molecular studies were perform...
|
PMID: 19821948
PDF is available here.
Abstract
Recent reports suggest that maternal immunization against low-frequency, platelet (PLT)-specific glycoprotein (GP) polymorphisms is a more common cause of neonatal alloimmune thrombocytopenia (NATP) than previously thought.
Serologic and molecular studies were perform...
|
PMID: 19821948
PDF is available here.
Abstract
Recent reports suggest that maternal immunization against low-frequency, platelet (PLT)-specific glycoprotein (GP) polymorphisms is a more common cause of neonatal alloimmune thrombocytopenia (NATP) than previously thought.
Serologic and molecular studies were perform...
|
PMID: 19821948
PDF is available here.
Abstract
Recent reports suggest that maternal immunization against low-frequency, platelet (PLT)-specific glycoprotein (GP) polymorphisms is a more common cause of neonatal alloimmune thrombocytopenia (NATP) than previously thought.
Serologic and molecular studies were perform...
|
PMID: 19821948
PDF is available here.
Abstract
Recent reports suggest that maternal immunization against low-frequency, platelet (PLT)-specific glycoprotein (GP) polymorphisms is a more common cause of neonatal alloimmune thrombocytopenia (NATP) than previously thought.
Serologic and molecular studies were perform...
|
PMID: 19821948
PDF is available here.
Abstract
Recent reports suggest that maternal immunization against low-frequency, platelet (PLT)-specific glycoprotein (GP) polymorphisms is a more common cause of neonatal alloimmune thrombocytopenia (NATP) than previously thought.
Serologic and molecular studies were perform...
|
PMID: 19821948
PDF is available here.
Abstract
Recent reports suggest that maternal immunization against low-frequency, platelet (PLT)-specific glycoprotein (GP) polymorphisms is a more common cause of neonatal alloimmune thrombocytopenia (NATP) than previously thought.
Serologic and molecular studies were perform...
|
PMID: 19821948
PDF is available here.
Abstract
Recent reports suggest that maternal immunization against low-frequency, platelet (PLT)-specific glycoprotein (GP) polymorphisms is a more common cause of neonatal alloimmune thrombocytopenia (NATP) than previously thought.
Serologic and molecular studies were perform...
|
PMID: 19821948
PDF is available here.
Abstract
Recent reports suggest that maternal immunization against low-frequency, platelet (PLT)-specific glycoprotein (GP) polymorphisms is a more common cause of neonatal alloimmune thrombocytopenia (NATP) than previously thought.
Serologic and molecular studies were perform...
|
PMID: 19821948
PDF is available here.
Abstract
Recent reports suggest that maternal immunization against low-frequency, platelet (PLT)-specific glycoprotein (GP) polymorphisms is a more common cause of neonatal alloimmune thrombocytopenia (NATP) than previously thought.
Serologic and molecular studies were perform...
|
PMID: 19821948
PDF is available here.
Abstract
Recent reports suggest that maternal immunization against low-frequency, platelet (PLT)-specific glycoprotein (GP) polymorphisms is a more common cause of neonatal alloimmune thrombocytopenia (NATP) than previously thought.
Serologic and molecular studies were perform...
|
PMID: 19821948
PDF is available here.
Abstract
Recent reports suggest that maternal immunization against low-frequency, platelet (PLT)-specific glycoprotein (GP) polymorphisms is a more common cause of neonatal alloimmune thrombocytopenia (NATP) than previously thought.
Serologic and molecular studies were perform...
|
PMID: 19821948
PDF is available here.
Abstract
Recent reports suggest that maternal immunization against low-frequency, platelet (PLT)-specific glycoprotein (GP) polymorphisms is a more common cause of neonatal alloimmune thrombocytopenia (NATP) than previously thought.
Serologic and molecular studies were perform...
|
PMID: 19821948
PDF is available here.
Abstract
Recent reports suggest that maternal immunization against low-frequency, platelet (PLT)-specific glycoprotein (GP) polymorphisms is a more common cause of neonatal alloimmune thrombocytopenia (NATP) than previously thought.
Serologic and molecular studies were perform...
|
PMID: 19821948
PDF is available here.
Abstract
Recent reports suggest that maternal immunization against low-frequency, platelet (PLT)-specific glycoprotein (GP) polymorphisms is a more common cause of neonatal alloimmune thrombocytopenia (NATP) than previously thought.
Serologic and molecular studies were perform...
|
PMID: 19821948
PDF is available here.
Abstract
Recent reports suggest that maternal immunization against low-frequency, platelet (PLT)-specific glycoprotein (GP) polymorphisms is a more common cause of neonatal alloimmune thrombocytopenia (NATP) than previously thought.
Serologic and molecular studies were perform...
|
PMID: 19821948
PDF is available here.
Abstract
Recent reports suggest that maternal immunization against low-frequency, platelet (PLT)-specific glycoprotein (GP) polymorphisms is a more common cause of neonatal alloimmune thrombocytopenia (NATP) than previously thought.
Serologic and molecular studies were perform...
|
PMID: 19821948
PDF is available here.
Abstract
Recent reports suggest that maternal immunization against low-frequency, platelet (PLT)-specific glycoprotein (GP) polymorphisms is a more common cause of neonatal alloimmune thrombocytopenia (NATP) than previously thought.
Serologic and molecular studies were perform...
|
PMID: 19821948
PDF is available here.
Abstract
We describe and demonstrate a technique for detection of cell surface antigens, with potential use in tissue antigen research. Briefly, a small volume of wetted chromatographic beads (specifically, 100 μL of Nickel-nitriloacetic acid (NTA) agarose) was bound to small quantities (specifically, ∼0....
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PMID: 20878645
PDF is available here.
Abstract
Liposomes prepared with biotinylated phospholipids and luminol entrapped were shown to be of 187 nm in size, 59% of which were unilamellar and with 43% luminol trapping efficiency. Liposome prepared from biotinylated phospholipids with a longer hydrophilic PEG2000 spacer, but not with the shorter hy...
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PMID: 20631437
PDF is available here.
Abstract
The electrooptical abilities of the microbial suspensions during a cells interaction with antibodies (ABs) of a different specificity have been studied on the example of the Azospirillum brasilense Sp245 cells and their interaction with the polyclonal monospecific and polyspecific antibodies. Measur...
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PMID: 20198920
PDF is available here.
Abstract
The presence of polyreactive immunoglobulins in sera may substantially influence on the accuracy of antibody affinity determination. In order to obtain precise values of antibody affinity one should apply one of two following ways. First, one should block polyreactive immunoglobulins with high conce...
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PMID: 20684229
PDF is available here.
Abstract
Our knowledge on antibody structure, in particular that of IgG1, largely used in the clinic, has progressed remarkably. However, some formidable challenges still remain to be confronted, among which the increase of a yet-limited antibody efficacy, the lowering of the frequency of serious clinical ad...
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PMID: 20035672
PDF is available here.
Abstract
We have designed a novel small molecule, designated as ARM-H, that has the potential to interfere with HIV survival through two mechanisms: (1) by recruiting antibodies to gp120-expressing virus particles and infected human cells, thus enhancing their uptake and destruction by the human immune syste...
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PMID: 19839582
PDF is available here.
Abstract
We developed a new experimental model for studying the mechanism of formalin fixation and antigen retrieval. Epitope mapping studies on nine antibodies useful for FFPE tissues revealed that each consisted of a contiguous stretch of amino acids in the native protein (linear epitope). Small peptides r...
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PMID: 19886757
PDF is available here.
Abstract
Immunocytokine (IC) hu14.18-IL2 is a fusion protein of humanized antidisialoganglioside (GD2) antibody (hu14.18) and interleukin (IL)-2. Sixty-one melanoma and neuroblastoma patients received IC in phase I/Ib studies. Patient sera were examined in ELISA to determine if an anti-IC antibody response oc...
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PMID: 19737959
PDF is available here.
Abstract
Signal size proportionality to a measurement value will be obtained in immunoassays only if the following four--theoretical--postulates are fulfilled: The immunochemical reaction behaviour (number and affinity of the epitopes) of the protein analyzed is identical and uniform for both the reference p...
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PMID: 1947751
PDF is available here.
Abstract
We identified CD22 expression on a blastic plasmacytoid dendritic cell (pDC) neoplasm presenting as a leukemia in a child. CD22 expression, as determined by the antibody s-HCL-1, was also noted on the neoplastic cells from three additional patients with blastic pDC tumors identified at our instituti...
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PMID: 19382197
PDF is available here.
Abstract
Guillain-Barré syndrome (GBS) is an acute immune-mediated polyradiculoneuropathy which can cause acute quadriplegia. Infection with micro-organisms, including Campylobacter jejuni (C. jejuni), Haemophilus influenzae, and Cytomegalovirus (CMV), is recognized as a main triggering event for the diseas...
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PMID: 19240270
PDF is available here.
Abstract
Alloimmunization is the formation of antibodies when there is an exposition of the individual to non-self antigens, as it occurs, for example, in the transfusion of incompatible blood and pregnancies, in whom the fetus express in its sanguineous cells antigens exclusively of paternal origin. This ar...
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PMID: 19684967
PDF is available here.