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Carbohydrate Sequence (0):
Recent article
Carbohydrate Sequence
Biochimica et Biophysica Acta (1808)10 2011
Abstract
In this work, the interaction between a synthetic analog of archaeal lipids and cholesterol was studied using Langmuir technique. The lipid, β-Mal(3)O(C(16+4))(2), contained phytanyl chains attached via two ether bonds to the sn-2 carbon of the glycerol backbone. The preliminary studies showed that...
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PMID: 21777564
PDF is available here.
PDF is available here.
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Biomacromolecules (12)6 2011
Abstract
We conducted a series of regioselective protection-deprotections of curdlan involving triphenylmethylation at C-6, phenylcarbamoylation at C-2 and C-4, and detriphenylmethylation. Subsequent glycosylation with a d-glucosamine-derived oxazoline, followed by deprotection gave rise to the branched curd...
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PMID: 21526779
PDF is available here.
PDF is available here.
Abstract
We have developed a new, broad-specificity "xyloglucan glycosynthase", selected from active-site mutants of a bacterial endoxyloglucanase, which catalyzed the synthesis of high molar mass polysaccharides, with complex side-chain structures, from suitable glycosyl fluoride donor substrates. The produ...
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PMID: 21618981
PDF is available here.
PDF is available here.
Abstract
These results are consistent with a C3 α-hydroxyalkyl radical in which the p-orbital harboring the unpaired electron spin at C3 is periplanar with the C-H bonds at both C2 and C4....
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PMID: 21513273
PDF is available here.
PDF is available here.
Abstract
A dimeric neomycin-neomycin conjugate 3 with a flexible linker, 2,2'-(ethylenedioxy)bis(ethylamine), has been synthesized and characterized. Dimer 3 can selectively bind to AT-rich DNA duplexes with high affinity. Biophysical studies have been performed between 3 and different nucleic acids with vary...
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PMID: 21524066
PDF is available here.
PDF is available here.
Innate Immunity (17)2 2011
Abstract
We present a structural and genetic analysis of the Y. pseudotuberculosis serotype O:1c OPS. Our results showed that this OPS has the same backbone as Y. pseudotuberculosis O:2b, but with a 3,6-dideoxy-D-ribo-hexofuranose (paratofuranose, Parf) side-branch instead of a 3,6-dideoxy-D-xylo-hexopyranos...
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PMID: 20418261
PDF is available here.
PDF is available here.
Abstract
In the process of adhesion, bacteria often carry proteins on their surface, adhesins, that bind to specific components of tissue cells or the extracellular matrix. In many cases these components are carbohydrate structures. The carbohydrate binding specificities of many bacteria have been uncovered o...
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PMID: 21557067
PDF is available here.
PDF is available here.
Abstract
We believe that daily intake of esculeoside A from tomato products can play a beneficial role in preventing the pathogenesis of atherosclerosis, the compound is not being used for preventive medicine due to the lack of information on methods for quantitative analysis and the content and stability of...
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PMID: 21395308
PDF is available here.
PDF is available here.
Abstract
We aim to identify potential glycans to therapeutically target this interaction and prevent SSL5-induced effects.
In addition to platelet activation experiments, flow cytometry, immunoprecipitation, surface plasmon resonance and a glycan binding array, were used to identify specific...
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PMID: 21552524
PDF is available here.
PDF is available here.
Abstract
We have shown that WalKR acts as the master controller of peptidoglycan metabolism, yet none of the identified regulon genes explain its requirement for cell viability. Transmission electron micrographs revealed cell wall thickening and aberrant division septa in the absence of WalKR, suggesting its...
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PMID: 21386961
PDF is available here.
PDF is available here.
Abstract
We show that the peptides bind on the surface of these cells and obtained clones that could even specifically bind to only specific regions of cells indicating restricted distribution of the epitopes. What was peculiar and interesting was that the epitopes were carbohydrate in nature. This gives ins...
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PMID: 21364990
PDF is available here.
PDF is available here.
BMC Biochemistry (12)1 2011
Abstract
We have reported the presence of highly sulfated dermatans in solitary ascidians from the orders Phlebobranchia (Phallusia nigra) and Stolidobranchia (Halocynthia pyriformis and Styela plicata). Despite the identical disaccharide backbone, consisting of [→4IdoA(2S)β-1→3GalNAcβ-1→], those pol...
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PMID: 21619699
PDF is available here.
PDF is available here.
Arzneimittel-Forschung (61)2 2011
Abstract
Dermal absorption of mucopolysaccharide polysulfate (MPS, the active ingredient of Hirudoid") in human and minipig was investigated by using 14C-labeled MPS. Three types of human and minipig skin samples were used: intact, dried and tape-stripped. At 24 h after application of 14C-MPS...
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PMID: 21428242
PDF is available here.
PDF is available here.
Abstract
We determined the O-chain repeating unit structure of the LPS from Halomonas alkaliantarctica strain CRSS, an haloalkaliphilic Gram-negative bacterium isolated from salt sediments of a saline lake in Cape Russell in Antarctic continent. The polysaccharide is constituted of the trisaccharidic repeati...
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PMID: 20890499
PDF is available here.
PDF is available here.
Biochemical Journal (431)2 2010
Abstract
We demonstrate that the neurotoxicity of BoNT/D depends on the presence of gangliosides by employing phrenic nerve hemidiaphragm preparations derived from mice expressing the gangliosides GM3, GM2, GM1 and GD1a, or only GM3 [a description of our use of ganglioside nomenclature is given in Svennerhol...
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PMID: 20704566
PDF is available here.
PDF is available here.
Carbohydrate Research (345)13 2010
Abstract
Exopolysaccharides (EPSs) from the Burkholderia genus are proposed to be involved in pathological conditions in humans, such as cystic fibrosis and septicemia, as well as in the stability of soil aggregates. Hence, considering that the conformational and dynamic aspects of such EPSs may influence th...
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PMID: 20633874
PDF is available here.
PDF is available here.
Carbohydrate Research (345)13 2010
Abstract
Based on the O-antigens (O-polysaccharides), one of the most variable cell constituents, 46 O-serogroups have been recognized in the Kauffmann-White serotyping scheme for Salmonella enterica. In this work, the structure of the O-polysaccharide and the genetic organization of the O-antigen gene clust...
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PMID: 20638049
PDF is available here.
PDF is available here.
Abstract
We describe a complete workflow for the analysis of protein N-glycans. The sample-preparation procedure, consisting of the release of the N-glycans by PNGase-F, followed by fluorescence labeling with 2-aminobenzamide and removal of excess label, was optimized to avoid alteration of the glycan sample...
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PMID: 20640408
PDF is available here.
PDF is available here.
Chemical Communications (46)31 2010
Abstract
MUC1 glycopeptide was efficiently coupled to glycosylphosphatidylinositol (GPI) derivatives by sortase A (SrtA), verifying that SrtA can accept sterically hindered glycopeptide as substrate for ligation with GPIs. This work has established a practical method for the chemoenzymatic synthesis of GPI-l...
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PMID: 20574591
PDF is available here.
PDF is available here.
Abstract
A low molecular weight fucogalactan, obtained from the brown seaweed Laminaria japonica, was separated into three fractions (LF1, LF2 and LF3) by DEAE-Sepharose FF column chromatography. All three fractions contained predominantly fucose, sulfate group and galactose. The results showed that the main...
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PMID: 20471412
PDF is available here.
PDF is available here.
Abstract
In this study, structural features of Tricholoma matsutake polysaccharide (TMP-A) were investigated by a combination of infrared (IR) spectra, gas chromatography-mass spectrometry (GC-MS), nuclear magnetic resonance (NMR) spectroscopy. The results indicated that TMP-A had a backbone of 1,4-beta-d-gl...
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PMID: 20430053
PDF is available here.
PDF is available here.
Abstract
The objective of this study was to prepare a series of chitosan-graft-polyethylenimine (chitosan-g-PEI) copolymers as gene carriers with high transfection efficiency and low cytotoxicity. Chitosan-g-PEIs with different molecular weights and segments were successfully synthesized by both oxidation an...
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PMID: 20824956
PDF is available here.
PDF is available here.
Protein Science (19)8 2010
Abstract
We report the kinetic mechanism and structure of the aminoglycoside phosphotransferase 2''-IVa (APH(2'')-IVa), an enzyme responsible for resistance to aminoglycoside antibiotics in clinical enterococcal and staphylococcal isolates. The enzyme operates via a Bi-Bi sequential mechanism in which the tw...
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PMID: 20556826
PDF is available here.
PDF is available here.
Plant Physiology (153)3 2010
Abstract
We describe here the lectin properties of the Arabidopsis (Arabidopsis thaliana) PP2-A1. Using a recombinant protein produced in Escherichia coli, we demonstrated binding to N-acetylglucosamine oligomers. Glycan array screening showed that PP2-A1 also bound to high-mannose N-glycans and 9-acyl-N-ace...
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PMID: 20442276
PDF is available here.
PDF is available here.
Biochemistry (Washington) (49)23 2010
Abstract
We report steady-state enzyme kinetics and hydrolysis-product analyses using anion-exchange chromatography and a novel rapid mass spectrometric profiling method of (18)O-labeled products obtained using H(2)(18)O as a solvent. The results obtained with oligosaccharide substrates show that although th...
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PMID: 20426480
PDF is available here.
PDF is available here.
Abstract
The antigenic O-polysaccharide component of the lipopolysaccharide produced by Escherichia coli serotype O71:H12 was analyzed by chemical composition, nuclear magnetic spectroscopy, and Smith-type periodate oxidation methods. It was determined to be a partially O-acetylated unbranched polymer of a r...
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PMID: 20555385
PDF is available here.
PDF is available here.
Abstract
The capsular polysaccharide (CPS) of Streptococcus suis serotype 2 was isolated, purified, chemically modified, and characterized. Sugar and absolute configuration analyses of the CPS gave the following composition: D-Gal, 3; D-Glc, 1; D-GlcNAc, 1; D-Neu5Ac, 1; L-Rha, 1. Sialic acid was found to be...
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PMID: 20555393
PDF is available here.
PDF is available here.
Glycobiology (20)6 2010
Abstract
We adapted this strategy to construct a modular approach to study the synthetic properties of individual glycosyltransferases expressed alone and in combinations. To this end, a set of expression vectors containing one to four putative glycosyltransferase genes from the lsg locus, lsgC-F, were trans...
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PMID: 20208062
PDF is available here.
PDF is available here.
Abstract
We developed a facile and high-yield synthesis of the corresponding disaccharide. Herein, the non-reducing disaccharide segment of phenolic glycolipid I from Mycobacterium leprae, O-(3,6-di-O-methyl-beta-D-glucopyranosyl)-(1-->4)-O-2,3-di-O-methyl-alpha-L-rhamnopyranose was synthesized by an improve...
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PMID: 20462755
PDF is available here.
PDF is available here.
Abstract
Many eukaryotic proteins are modified with a glycosylphosphatidylinositol (GPI) anchor at their C-termini. This post-translational modification causes these proteins to be noncovalently tethered to the plasma membrane. The synthesis of truncated GPI anchor analogues is reported; these compounds were...
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PMID: 20380381
PDF is available here.
PDF is available here.
Abstract
D-Galactofuranosyl-containing conjugates are ubiquitous in many pathogenic microorganisms, but completely absent from mammals. As they may constitute interesting pharmacophores, recent works have been dedicated to their preparation. Besides well-reported chemical procedures, enzymatic approaches are...
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PMID: 20401386
PDF is available here.
PDF is available here.
Carbohydrate Research (345)7 2010
Abstract
The Cronobacter spp., previously known as Enterobacter sakazakii, are Gram-negative enterobacterial pathogens that can cause necrotizing enterocolitis, meningitis, and septicemia with a high mortality rate in neonates. The O-specific polysaccharide (O-PS) was isolated from Cronobacter sakazakii stra...
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PMID: 20206918
PDF is available here.
PDF is available here.
Carbohydrate Research (345)7 2010
Abstract
An O-polysaccharide was obtained by mild acid degradation of the lipopolysaccharide of Salmonella enterica O41, and the following structure of the O-unit was determined by chemical analyses along with 1D and 2D (1)H and (13)C NMR spectroscopy: -->2)-beta-d-Manp-(1-->4)-alpha-d-Glcp-(1-->3)-alpha-l-Q...
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PMID: 20199769
PDF is available here.
PDF is available here.
Abstract
I backbone, a pectin domain, using Arabidopsis thaliana seed mucilage-derived rhamnogalacturonan I oligosaccharides--BSA conjugates. Two monoclonal antibodies, designated INRA-RU1 and INRA-RU2, selected for further characterization, were specific for the backbone of rhamnogalacturonan I, displaying...
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PMID: 20309579
PDF is available here.
PDF is available here.
Abstract
A novel polysaccharide (GBP50S2) with antioxidant activity was isolated from Ginkgo biloba. The structure of GBP50S2 was elucidated on the basis of physico-chemical and instrumental analyses, and its average molecular weight (Mw=2.30x10(5)) was determined by gel permeation chromatography. The backbo...
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PMID: 20153363
PDF is available here.
PDF is available here.
Biochemistry (New York) (75)5 2010
Abstract
An acidic O-specific polysaccharide containing L-rhamnose, 2-acetamido-2-deoxy-D-galactose, 2,6-dideoxy-2-(N-acetyl-L-threonine)amino-D-galactose, and 2-acetamido-2-deoxy-D-mannuronic acid was obtained by mild acid degradation of the lipopolysaccharide of the marine bacterium Pseudoalteromonas agari...
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PMID: 20632942
PDF is available here.
PDF is available here.
Biochemistry (New York) (75)5 2010
Abstract
Antigenic differences were revealed between the cell wall outer membrane lipopolysaccharides and the capsular high molecular weight bioglycans for a typical strain of the nitrogen-fixing rhizobacterium Azospirillum lipoferum Sp59b using antibodies prepared against the homologous lipopolysaccharide a...
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PMID: 20632940
PDF is available here.
PDF is available here.
Chemistry & Biology (17)4 2010
Abstract
The broadly neutralizing antibody 2G12 recognizes a conserved cluster of high-mannose glycans on the surface envelope spike of HIV, suggesting that the "glycan shield" defense of the virus can be breached and may, under the right circumstances, serve as a vaccine target. In an attempt to recreate fe...
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PMID: 20416507
PDF is available here.
PDF is available here.
Abstract
A versatile synthetic procedure to construct series of high- and low-density homo- and heteroglycoclusters is reported. The binding properties of these synthetic multivalent glycoconjugates to concanavalin A (Con A), a model lectin, have been assessed by using a range of competitive and non-competit...
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PMID: 20449489
PDF is available here.
PDF is available here.
Abstract
Endohexosaminidase D, a family 85 glycoside hydrolase from S. pneumoniae and the first endohexosaminidase to be discovered, is found to be capable of catalysing the glycosylation of a glycosyl amino acid bearing a GlcNAc residue using a variety of N-glycan oxazoline donors. Although enzyme-catalysed...
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PMID: 20449490
PDF is available here.
PDF is available here.
Journal of Antibiotics (63)4 2010
Abstract
The structures of nosokomycins A, B, C and D, new anti-methicillin-resistant Staphylococcus aureus antibiotics produced by Streptomyces sp. K04-0144, were elucidated by spectroscopic studies including various NMR experiments. Nosokomycins A, B, C and D are new members of the moenomycin family consis...
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PMID: 20203703
PDF is available here.
PDF is available here.
Journal of Antibiotics (63)4 2010
Abstract
The in vivo-mimic assay system using silkworm larvae was used as a screening tool to discover antibiotics against methicillin-resistant Staphylococcus aureus (MRSA). Microbial culture broths were screened in this in vivo-mimic assay system and a culture broth of Streptomyces sp. K04-0144 was selecte...
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PMID: 20186168
PDF is available here.
PDF is available here.
Abstract
A new triterpenoid saponin was isolated from Pulsatilla cernua, along with eight known triterpenoids and triterpenoid glycosides. The new compound was identified as 3-O-beta-D-glucopyranosyl-(1-->4)-alpha-L-arabinopyranosyl-bayogenin-28-alpha-L-rhamnopyranosyl-(1-->4)-beta-D-glucopyranosyl-(1-->6)-b...
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PMID: 20336019
PDF is available here.
PDF is available here.
Carbohydrate Research (345)3 2010
Abstract
We performed N-glycosylation profiling of turtle egg yolk by using HPLC mapping in conjunction with mass spectrometric methods and thereby demonstrated that the alpha-Galp-(1-->4)-Galp groups are displayed on approximately 38% of total N-glycans. Our findings suggest that the ability to express the...
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PMID: 20044081
PDF is available here.
PDF is available here.
Abstract
I (3) and catalpol (4), two saponins, ilwensisaponin A (5) and C (6), and a phenylethanoid glycoside, verbascoside (7), in Verbascum mucronatum. Their structures were elucidated by spectral techniques. The aqueous extract and fractions including the phenylethanoid glycoside 7 showed DPPH scavenger e...
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PMID: 21319708
PDF is available here.
PDF is available here.
Abstract
We were able to analyze interactions between sugar chains and proteins such as enzymes and lectins in detail. Interestingly, our studies reveal that some enzymes recognize a wider region of the substrate than anticipated....
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PMID: 20431262
PDF is available here.
PDF is available here.
Abstract
Lipopolysaccharides (LPSs) from Taylorella equigenitalis, the causative agent of contagious equine metritis, and T. asinigenitalis were compared by sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE). Lipopolysaccharide profiles of 11 T. equigenitalis strains were similar, but diff...
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PMID: 20357953
PDF is available here.
PDF is available here.
Abstract
We are now able to identify all isomeric glycoforms in very heterogeneous LPS preparations....
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PMID: 19882122
PDF is available here.
PDF is available here.
Abstract
We sequenced and characterized the O-antigen biosynthetic gene cluster of Escherichia coli serotype O86. Four glycosyltransferases encoded by the genes within the cluster were cloned and overexpressed. In vitro reconstitution of the O-repeating unit of E. coli 086 was achieved via using these enzyme...
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PMID: 19882123
PDF is available here.
PDF is available here.
Abstract
We report a glycoproteomics study on the identification of even low abundant glycoproteins, including the localization of N-glycosylation sites and the glycan profiling in human sera from healthy and myocarditis affected donors. The strategy is simply based on proteolytic digestion of total serum pr...
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PMID: 20065524
PDF is available here.
PDF is available here.
Abstract
We present an overview of recent advances in protecting groups influencing stereoselectivity in glycosylation reactions, including participating protecting groups, and conformation-constraining protecting groups in general....
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PMID: 20966873
PDF is available here.
PDF is available here.
Abstract
The full structure of the lipopolysaccharide core of bacteria Shigella flexneri types 2a and 5b, the causative agents of bacillary dysentery (shigellosis), was established by chemical methods, high-resolution electrospray ionization mass spectrometry, and two-dimensional NMR spectroscopy. The struct...
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PMID: 20644600
PDF is available here.
PDF is available here.
Abstract
Mass spectrometric studies are now playing a leading role in the elucidation of lipopolysaccharide (LPS) structures through the characterization of antigenic polysaccharides, core oligosaccharides and lipid A components including LPS genetic modifications. The conventional MS and MS/MS analyses toge...
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PMID: 20589944
PDF is available here.
PDF is available here.
Abstract
Natural derivatives and synthetic analogues of lipopolysaccharide are potent stimulators of the mammalian immune system. Retained adjuvant activity with reduced toxicity was obtained by the development of monophosphoryl lipid A (MPL((R))), which is approved for use in several vaccine products. Ongoi...
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PMID: 20593273
PDF is available here.
PDF is available here.
Abstract
The O antigen, consisting of many repeats of an oligosaccharide unit, is part of the lipopolysaccharide (LPS) in the outer membrane of Gram-negative bacteria. It is on the cell surface and appears to be a major target for both immune system and bacteriophages, and therefore becomes one of the most v...
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PMID: 20593265
PDF is available here.
PDF is available here.
Abstract
We therefore developed a subunit vaccine by combining detoxified J5 LPS (J5 dLPS) with the outer membrane protein (OMP) from group B N. meningitidis. This vaccine was highly efficacious in experimental models of sepsis and progressed to phase 1 clinical trial. While well-tolerated, this vaccine indu...
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PMID: 20593272
PDF is available here.
PDF is available here.
Abstract
Pseudomonas aeruginosa (PA) is a ubiquitous environmental Gram-negative bacterium found in soil and water. This opportunistic pathogen can cause infections in individuals with impaired phagocytic function, such as those with burns, exposure to chemotherapy, or cystic fibrosis (CF). PA infects the lu...
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PMID: 20593270
PDF is available here.
PDF is available here.
Abstract
We describe the structures of the LPSs from symbiont members of the Rhizobiales, the genetics and mechanism of their biosynthesis, the modifications that occur during symbiosis, and their possible functions....
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PMID: 20593275
PDF is available here.
PDF is available here.
Abstract
Bacterial lipopolysaccharides (LPSs) are the major component of the outer membrane of Gram-negative bacteria. They have a structural role since they contribute to the cellular rigidity by increasing the strength of cell wall and mediating contacts with the external environment that can induce struct...
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PMID: 20593263
PDF is available here.
PDF is available here.
Abstract
Gram negative bacterial endotoxin is a biological pyrogen that causes fever when introduced intravenously. The endotoxin, also known as lipopolysaccharide (LPS), is found in the outer membrane of Gram-negative bacteria. During Gram-negative sepsis, endotoxin stimulates host macrophages to release in...
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PMID: 20593268
PDF is available here.
PDF is available here.