Abstract
We prepared eight chimeric enzymes. Based on the results of the relationship between Ca(2+) and hydrolysis activity, the region of amino-acid residues 244-349 of StMan was responsible for a Ca(2+) sensitive site.
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PMID: 21601016
PDF is available here.
Abstract
We designed a novel VLR protein (called dVLR) containing six LRR repeats based on a sea lamprey receptor sequence. The designed protein was expressed in Escherichia coli in a soluble, native form and showed very favorable biophysical properties. Recombinant dVLR is monomeric in solution and preserve...
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PMID: 21621013
PDF is available here.
Abstract
We perform an EPR study on the role played by the membrane composition in tuning the interaction between lipid bilayers and two peptides, gH626-644 and gB632-650, that are highly fusogenic fragments of the gH and gB glycoproteins of herpes simplex virus. Our results show that both peptides interact...
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PMID: 21798233
PDF is available here.
Abstract
The survival motor neuron (SMN) protein plays an essential role in the assembly of uridine-rich small nuclear ribonuclear protein complexes. Phosphorylation of SMN can regulate its function, stability, and sub-cellular localization. This study shows that protein kinase A (PKA) phosph...
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PMID: 21609790
PDF is available here.
Abstract
Infectious bronchitis virus (IBV) Cal99 variant was isolated from the kidneys of seven 2-5-mo-old game chickens with nephritis and respiratory disease. IBV Cal99 variant is usually associated with respiratory disease in broiler chickens in California. Macroscopically, the majority of...
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PMID: 22017040
PDF is available here.
Abstract
The study aimed to construct the amplicon vector of HSV-1 strain HF and explore its universal package function between different serotypes of HSV. OriS and pac elements were obtained by enzyme digestion from the Plasmid BAC-HSV-1 strain HF and sequenced. With red fluorescence (DsRed)...
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PMID: 21998950
PDF is available here.
Abstract
To identify and trace the pathogen of acute hemorrhagic conjunctivitis (AHC) epidemic in Zhejiang Province in 2010. Viral nucleic acid of Enterovirus (EV) and Coxsackievirus A24 variant (CA24v) were directly detected by real-time RT-PCR from the conjunctival swab collected from suspe...
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PMID: 21998952
PDF is available here.
Abstract
The purpose of this study was to compare the whole genome sequences and replication dynamics in cell cultures of two Avian leukosis viruses of subgroup B (ALV) isolates, SDAU09E3 and SDAU09C2. Comparison of the amino acid sequences indicated that the gp85 identity of these two subgro...
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PMID: 21998957
PDF is available here.
Abstract
In order to confirm the cause of the outbreak of aseptic meningitis in Zhejiang Province in 2002-2004, trace the pathogen and analyze the molecular characteristics, 271 cerebrospinal fluid (CSF) and faeces specimens were collected from suspected patients. The virus strains from the s...
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PMID: 21998959
PDF is available here.
Abstract
We have examined the in vivo amyloid reactivity of seven heparin-binding peptides by using single photon emission and X-ray computed tomographic imaging, microautoradiography, and tissue biodistribution measurements. All of the peptides bound amyloid deposits within 1 h post-injection, but the exten...
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PMID: 21807994
PDF is available here.
Abstract
We applied a recently developed reprogramming approach to generate a novel in vitro human RTT model. Induced pluripotent stem cells (iPSCs) were derived from RTT fibroblasts by overexpressing the reprogramming factors OCT4, SOX2, KLF4, and MYC. Intriguingly, whereas some iPSCs maintained X chromosom...
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PMID: 21807996
PDF is available here.
Melanie M Merk,
Swen S Zierow,
Lin L Leng,
Rituparna R Das,
Xin X Du,
Wibke W Schulte,
Juan J Fan,
Hongqi H Lue,
Yibang Y Chen,
Huabao H Xiong,
Frederic F Chagnon,
Jürgen J Bernhagen,
Elias E Lolis,
Gil G Mor,
Olivier O Lesur and
Richard R Bucala
Abstract
We hypothesized that these observations may be explained by a second MIF-like ligand, and we considered a probable candidate to be the protein encoded by the homologous, D-dopachrome tautomerase (D-DT) gene. We show that recombinant D-DT protein binds CD74 with high affinity, leading to activation o...
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PMID: 21817065
PDF is available here.
Abstract
We know Arabidopsis suecica and Arabidopsis kamchatica as fully stabilized allopolyploid species. Both species evolved during Pleistocene glaciation and deglaciation cycles in Fennoscandinavia and the amphi-Beringian region, respectively. These hybrid studies were conducted either on a phylogeograph...
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PMID: 21825128
PDF is available here.
Abstract
We recently introduced the enhanced aromatic sequon as a family of portable structural motifs that are stabilized upon glycosylation in specific reverse turn contexts: a five-residue type I β-turn harboring a G1 β-bulge (using a Phe-Yyy-Asn-Xxx-Thr sequon) and a type II β-turn within a six-residu...
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PMID: 21825145
PDF is available here.
Abstract
We identified a third candidate early domestication gene and conducted haplotype analyses of all three genes to address a recent, controversial hypothesis about the origin of cultivated sunflower. Although the scientific consensus had long been that sunflower was domesticated once in eastern North A...
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PMID: 21844335
PDF is available here.
Abstract
We report that the resistance to the Bt toxin Cry1Ac in the cabbage looper, Trichoplusia ni, evolved in greenhouses, is associated with differential alteration of two midgut aminopeptidases N, APN1 and APN6, conferred by a trans-regulatory mechanism. Biochemical, proteomic, and molecular analyses sh...
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PMID: 21844358
PDF is available here.
Abstract
We explore the functional significance of an unprecedented example of regulatory state conservation from the cnidarian Nematostella to Drosophila, sea urchin, fish, and mammals. Our probe is a deeply conserved cis-regulatory DNA module of the SRY-box B2 (soxB2), recognizable at the sequence level ac...
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PMID: 21844364
PDF is available here.
Abstract
We show that symbiosis-induced calcium changes occur in both the nucleoplasm and the perinuclear cytoplasm and seem to originate from the nuclear membranes. Reaction diffusion simulations suggest that spike generation within the nucleoplasm is not possible through transmission of a calcium wave from...
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PMID: 21825141
PDF is available here.
Matthew M Taylor,
Sharon S Graw,
Gianfranco G Sinagra,
Carl C Barnes,
Dobromir D Slavov,
Francesca F Brun,
Bruno B Pinamonti,
Ernesto E EE Salcedo,
William W Sauer,
Stylianos S Pyxaras,
Brian B Anderson,
Bernd B Simon,
Julius J Bogomolovas,
Siegfried S Labeit,
Henk H Granzier and
Luisa L Mestroni
Abstract
We evaluated the cardiomyopathy gene titin (TTN) as a candidate ARVC gene because of its proximity to an ARVC locus at position 2q32 and the connection of the titin protein to the transitional junction at intercalated disks. Methods and Results- All 312 titin exons known to be expressed in human car...
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PMID: 21810661
PDF is available here.
Abstract
We report cultures of this group and describe the class, Archaeorhizomycetes, phylogenetically placed within subphylum Taphrinomycotina in the Ascomycota. Archaeorhizomycetes comprises hundreds of cryptically reproducing filamentous species that do not form recognizable mycorrhizal structures and ha...
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PMID: 21836015
PDF is available here.
E Toby ET Kiers,
Marie M Duhamel,
Yugandhar Y Beesetty,
Jerry A JA Mensah,
Oscar O Franken,
Erik E Verbruggen,
Carl R CR Fellbaum,
George A GA Kowalchuk,
Miranda M MM Hart,
Alberto A Bago,
Todd M TM Palmer,
Stuart A SA West,
Philippe P Vandenkoornhuyse,
Jan J Jansa and
Heike H Bücking
Abstract
We manipulated cooperation in plants and fungal partners to show that plants can detect, discriminate, and reward the best fungal partners with more carbohydrates. In turn, their fungal partners enforce cooperation by increasing nutrient transfer only to those roots providing more carbohydrates. On...
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PMID: 21836016
PDF is available here.
Abstract
We report the crystal structure of a motor domain dimer in complex with its tail domain at 2.2 angstroms and compare it with a structure of the motor domain alone at 2.7 angstroms. These structures indicate that neither an induced conformational change nor steric blocking is the cause of inhibition....
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PMID: 21836017
PDF is available here.
Abstract
We combined autozygome analysis with exome sequencing to identify a homozygous truncating mutation in dedicator of cytokinesis 6 gene (DOCK6) which encodes an atypical guanidine exchange factor (GEF) known to activate two members of the Rho GTPase family: Cdc42 and Rac1. Another homozygous truncatin...
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PMID: 21820096
PDF is available here.
Abstract
We investigated a large family affected with autosomal-dominant adermatoglyphia (absence of fingerprints) also known as the "immigration delay disease." Using linkage and haplotype analyses, we mapped the disease phenotype to 4q22. One of the genes located in this interval is SMARCAD1, a member of t...
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PMID: 21820097
PDF is available here.