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Nitrogen Isotopes (1):
Recent article
Nitrogen Isotopes
Abstract
We show that bedrock comprises a hitherto overlooked source of ecologically available N to forests. We report that the N content of soils and forest foliage on N-rich metasedimentary rocks (350-950 mg N kg(-1)) is elevated by more than 50% compared with similar temperate forest sites underlain...
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PMID: 21886160
PDF is available here.
PDF is available here.
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Abstract
A stable isotope dilution assay (SIDA) for the Alternaria mycotoxin tenuazonic acid was developed. Therefore, [(13)C(6),(15)N]-tenuazonic acid was synthesized from [(13)C(6),(15)N]-isoleucine by Dieckmann intramolecular cyclization after acetoacetylation with diketene. The synthesized [(13)C(6),(15)N...
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PMID: 21370870
PDF is available here.
PDF is available here.
Molecular Biology Reports (36 Suppl 1) 2010
Abstract
Abstract
SH3 is a ubiquitous domain mediating protein-protein interactions. Recent solution NMR structural studies have shown that a proline-rich peptide is capable of binding to the human vinexin SH3 domain. Here, an orthogonal amber tRNA/tRNA synthetase pair for (15)N/(19)F-trifluoromethyl-phenylalanine ((...
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PMID: 20946873
PDF is available here.
PDF is available here.
Abstract
We utilize an experimental framework based on competition theory to test whether plants partition resources via classical niche differentiation or via plasticity in resource use. We explore two alternatives: niche preemption, in which individuals respond to a superior competitor by switching to an a...
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PMID: 21141186
PDF is available here.
PDF is available here.
Abstract
We report here the (1)H, (15)N and (13)C assignments for the reduced form of a thiol-disulphide oxidoreductase from the Antarctic bacterium Pseudoalteromonas haloplanktis TAC125....
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PMID: 20455034
PDF is available here.
PDF is available here.
Ecological Applications (20)7 2010
Abstract
We used the isotopic composition of nitrate (delta15N-NO3- and delta18O-NO3-) in conjunction with a simple numerical model to examine the spatial and temporal variability of nitrate (NO3-) export across a land-use gradient and how agricultural and urban development affects net removal mechanisms. In...
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PMID: 21049883
PDF is available here.
PDF is available here.
Abstract
We present a simple method, ARTSY, for extracting ¹J(NH) couplings and ¹H-¹⁵N RDCs from an interleaved set of two-dimensional ¹H-¹⁵N TROSY-HSQC spectra, based on the principle of quantitative J correlation. The primary advantage of the ARTSY method over other methods is the ability to measu...
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PMID: 20694505
PDF is available here.
PDF is available here.
Abstract
We provide analytical solutions for the S-REDOR and S-RESPDOR dephasing curves. These solutions facilitate the measurement of hetero-nuclear distances from experimental data....
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PMID: 20577687
PDF is available here.
PDF is available here.
Abstract
We investigated the incorporation of (15)N from (15)NH(4)Cl into amino acids using an in vitro tissue culture system. Fat body or midgut tissues from female mosquitoes were incubated in an Aedes saline solution supplemented with glucose and (15)NH(4)Cl for 10-40min. The media were then mixed with de...
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PMID: 20206632
PDF is available here.
PDF is available here.
Abstract
Nitrogen (N) retention and transformation in the Szczecin Lagoon, southern Baltic Sea, were studied by means of budget calculations and stable isotope data of dissolved and particulate matter. Two stations, one located at the main outlet of the lagoon (Swina Strait) and the other 100 km to the south...
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PMID: 20672205
PDF is available here.
PDF is available here.
Abstract
We applied two different approaches to investigate the impact of diazotrophy on nitrogen stable isotope signatures in nitrate and particulate organic nitrogen (PON) of the food-web constituents. The first approach, used during the Poseidon cruise 348 in the Mauritanian upwelling, investigated the lo...
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PMID: 20706897
PDF is available here.
PDF is available here.
Abstract
Abstract
We develop and evaluate a macroalgal bioassay tool for monitoring the spatial extent of dissolved wastes loaded from offshore fish farms into the marine coastal ecosystem. This tool is based on the analysis of the nitrogen stable isotope ratio (delta(15)N) in tissues of several benthic primary produ...
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PMID: 20621769
PDF is available here.
PDF is available here.
Abstract
The combined use of two-dimensional NMR correlation experiments and gauge including atomic orbital density functional theory in (13)C NMR chemical shift (CS) calculations allowed reliable and simple structural determination of regioisomeric heterocyclic systems that originate from the reactions of a...
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PMID: 20589725
PDF is available here.
PDF is available here.
Abstract
We found that, depending on the triazole precursor, only one regioisomer resulted, either of the 1 or 2 series. We also observed that these two structural surrogates could be unambiguously differentiated by indirectly measuring their (15)N chemical shifts by (1)H-(15)N HMBC experiments. The occasion...
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PMID: 20589727
PDF is available here.
PDF is available here.
Abstract
We present here a set of (13)C-direct detected NMR experiments to facilitate the resonance assignment of RNA oligonucleotides. Three experiments have been developed: (1) the (H)CC-TOCSY-experiment utilizing a virtual decoupling scheme to assign the intraresidual ribose (13)C-spins, (2) the (H)CPC-ex...
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PMID: 20544375
PDF is available here.
PDF is available here.
Abstract
We present two novel (15)N direct-detection experiments. The CAN experiment sequentially connects amide (15)N resonances using (13)C(alpha) chemical shift matching, and the CON experiment connects the preceding (13)C' nuclei. When starting from the same carbon polarization, the intensities of nitrog...
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PMID: 20556482
PDF is available here.
PDF is available here.
Abstract
Unambiguous vibrational band assignments have been made to cyclic nitramine hexahydro-1,3,5-trinitro-s-triazine, commonly known as the alpha-phase of RDX or alpha-RDX, with the use of (13)C and (15)N (on ring) enriched isotopic RDX analogues. Vibrational spectra were collected using Raman and IR spe...
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PMID: 20381411
PDF is available here.
PDF is available here.
Abstract
Although glutamine is considered the main precursor for citrulline synthesis, the current literature does not differentiate between the contribution of glutamine carbon skeleton vs. nonspecific nitrogen (i.e., ammonia) and carbon derived from glutamine oxidation. To elucidate the role of glutamine a...
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PMID: 20407005
PDF is available here.
PDF is available here.
Abstract
We analyzed the stable carbon (delta(13)C), hydrogen (deltaD), and nitrogen (delta(15)N) isotope composition of peregrine eggs collected from urban and nonurban habitats. We found that delta(13)C values were significantly higher in urban versus nonurban eggs, suggesting that urban peregrines indirec...
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PMID: 20540532
PDF is available here.
PDF is available here.
New Phytologist (188)2 2010
Abstract
We know little about the ecophysiology of arbuscular mycorrhizal mycoheterotrophs. Here, we identify the mycorrhizal fungi of two unrelated mycoheterotrophs and one putative partial mycoheterotroph that form arbuscular mycorrhizas, and analyse their carbon (C) and nitrogen (N) isotope signatures. ...
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PMID: 20618915
PDF is available here.
PDF is available here.
New Phytologist (187)2 2010
Abstract
We studied N cycling under ambient and elevated CO(2) concentrations (600 micromol mol(-1)), and ambient and elevated temperature (1.5 : 3.0 degrees C warmer day:night) in a full factorial semiarid grassland field experiment in Wyoming, USA. We measured soil inorganic N, plant and microbial N pool s...
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PMID: 20487311
PDF is available here.
PDF is available here.
New Phytologist (187)2 2010
Abstract
We studied N cycling under ambient and elevated CO(2) concentrations (600 micromol mol(-1)), and ambient and elevated temperature (1.5 : 3.0 degrees C warmer day:night) in a full factorial semiarid grassland field experiment in Wyoming, USA. We measured soil inorganic N, plant and microbial N pool s...
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PMID: 20487311
PDF is available here.
PDF is available here.
New Phytologist (187)2 2010
Abstract
We studied N cycling under ambient and elevated CO(2) concentrations (600 micromol mol(-1)), and ambient and elevated temperature (1.5 : 3.0 degrees C warmer day:night) in a full factorial semiarid grassland field experiment in Wyoming, USA. We measured soil inorganic N, plant and microbial N pool s...
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PMID: 20487311
PDF is available here.
PDF is available here.
New Phytologist (187)2 2010
Abstract
We studied N cycling under ambient and elevated CO(2) concentrations (600 micromol mol(-1)), and ambient and elevated temperature (1.5 : 3.0 degrees C warmer day:night) in a full factorial semiarid grassland field experiment in Wyoming, USA. We measured soil inorganic N, plant and microbial N pool s...
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PMID: 20487311
PDF is available here.
PDF is available here.
New Phytologist (187)2 2010
Abstract
We studied N cycling under ambient and elevated CO(2) concentrations (600 micromol mol(-1)), and ambient and elevated temperature (1.5 : 3.0 degrees C warmer day:night) in a full factorial semiarid grassland field experiment in Wyoming, USA. We measured soil inorganic N, plant and microbial N pool s...
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PMID: 20487311
PDF is available here.
PDF is available here.
New Phytologist (187)2 2010
Abstract
We studied N cycling under ambient and elevated CO(2) concentrations (600 micromol mol(-1)), and ambient and elevated temperature (1.5 : 3.0 degrees C warmer day:night) in a full factorial semiarid grassland field experiment in Wyoming, USA. We measured soil inorganic N, plant and microbial N pool s...
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PMID: 20487311
PDF is available here.
PDF is available here.
New Phytologist (187)2 2010
Abstract
We studied N cycling under ambient and elevated CO(2) concentrations (600 micromol mol(-1)), and ambient and elevated temperature (1.5 : 3.0 degrees C warmer day:night) in a full factorial semiarid grassland field experiment in Wyoming, USA. We measured soil inorganic N, plant and microbial N pool s...
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PMID: 20487311
PDF is available here.
PDF is available here.
New Phytologist (187)2 2010
Abstract
We studied N cycling under ambient and elevated CO(2) concentrations (600 micromol mol(-1)), and ambient and elevated temperature (1.5 : 3.0 degrees C warmer day:night) in a full factorial semiarid grassland field experiment in Wyoming, USA. We measured soil inorganic N, plant and microbial N pool s...
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PMID: 20487311
PDF is available here.
PDF is available here.
New Phytologist (187)2 2010
Abstract
We studied N cycling under ambient and elevated CO(2) concentrations (600 micromol mol(-1)), and ambient and elevated temperature (1.5 : 3.0 degrees C warmer day:night) in a full factorial semiarid grassland field experiment in Wyoming, USA. We measured soil inorganic N, plant and microbial N pool s...
|
PMID: 20487311
PDF is available here.
PDF is available here.
New Phytologist (187)2 2010
Abstract
We studied N cycling under ambient and elevated CO(2) concentrations (600 micromol mol(-1)), and ambient and elevated temperature (1.5 : 3.0 degrees C warmer day:night) in a full factorial semiarid grassland field experiment in Wyoming, USA. We measured soil inorganic N, plant and microbial N pool s...
|
PMID: 20487311
PDF is available here.
PDF is available here.
New Phytologist (187)2 2010
Abstract
We studied N cycling under ambient and elevated CO(2) concentrations (600 micromol mol(-1)), and ambient and elevated temperature (1.5 : 3.0 degrees C warmer day:night) in a full factorial semiarid grassland field experiment in Wyoming, USA. We measured soil inorganic N, plant and microbial N pool s...
|
PMID: 20487311
PDF is available here.
PDF is available here.
New Phytologist (187)2 2010
Abstract
We studied N cycling under ambient and elevated CO(2) concentrations (600 micromol mol(-1)), and ambient and elevated temperature (1.5 : 3.0 degrees C warmer day:night) in a full factorial semiarid grassland field experiment in Wyoming, USA. We measured soil inorganic N, plant and microbial N pool s...
|
PMID: 20487311
PDF is available here.
PDF is available here.
New Phytologist (187)2 2010
Abstract
We studied N cycling under ambient and elevated CO(2) concentrations (600 micromol mol(-1)), and ambient and elevated temperature (1.5 : 3.0 degrees C warmer day:night) in a full factorial semiarid grassland field experiment in Wyoming, USA. We measured soil inorganic N, plant and microbial N pool s...
|
PMID: 20487311
PDF is available here.
PDF is available here.
New Phytologist (187)2 2010
Abstract
We studied N cycling under ambient and elevated CO(2) concentrations (600 micromol mol(-1)), and ambient and elevated temperature (1.5 : 3.0 degrees C warmer day:night) in a full factorial semiarid grassland field experiment in Wyoming, USA. We measured soil inorganic N, plant and microbial N pool s...
|
PMID: 20487311
PDF is available here.
PDF is available here.
New Phytologist (187)2 2010
Abstract
We studied N cycling under ambient and elevated CO(2) concentrations (600 micromol mol(-1)), and ambient and elevated temperature (1.5 : 3.0 degrees C warmer day:night) in a full factorial semiarid grassland field experiment in Wyoming, USA. We measured soil inorganic N, plant and microbial N pool s...
|
PMID: 20487311
PDF is available here.
PDF is available here.
New Phytologist (187)2 2010
Abstract
We studied N cycling under ambient and elevated CO(2) concentrations (600 micromol mol(-1)), and ambient and elevated temperature (1.5 : 3.0 degrees C warmer day:night) in a full factorial semiarid grassland field experiment in Wyoming, USA. We measured soil inorganic N, plant and microbial N pool s...
|
PMID: 20487311
PDF is available here.
PDF is available here.
New Phytologist (187)2 2010
Abstract
We studied N cycling under ambient and elevated CO(2) concentrations (600 micromol mol(-1)), and ambient and elevated temperature (1.5 : 3.0 degrees C warmer day:night) in a full factorial semiarid grassland field experiment in Wyoming, USA. We measured soil inorganic N, plant and microbial N pool s...
|
PMID: 20487311
PDF is available here.
PDF is available here.
New Phytologist (187)2 2010
Abstract
We studied N cycling under ambient and elevated CO(2) concentrations (600 micromol mol(-1)), and ambient and elevated temperature (1.5 : 3.0 degrees C warmer day:night) in a full factorial semiarid grassland field experiment in Wyoming, USA. We measured soil inorganic N, plant and microbial N pool s...
|
PMID: 20487311
PDF is available here.
PDF is available here.
New Phytologist (187)2 2010
Abstract
We studied N cycling under ambient and elevated CO(2) concentrations (600 micromol mol(-1)), and ambient and elevated temperature (1.5 : 3.0 degrees C warmer day:night) in a full factorial semiarid grassland field experiment in Wyoming, USA. We measured soil inorganic N, plant and microbial N pool s...
|
PMID: 20487311
PDF is available here.
PDF is available here.
New Phytologist (187)2 2010
Abstract
We studied N cycling under ambient and elevated CO(2) concentrations (600 micromol mol(-1)), and ambient and elevated temperature (1.5 : 3.0 degrees C warmer day:night) in a full factorial semiarid grassland field experiment in Wyoming, USA. We measured soil inorganic N, plant and microbial N pool s...
|
PMID: 20487311
PDF is available here.
PDF is available here.
New Phytologist (187)2 2010
Abstract
We studied N cycling under ambient and elevated CO(2) concentrations (600 micromol mol(-1)), and ambient and elevated temperature (1.5 : 3.0 degrees C warmer day:night) in a full factorial semiarid grassland field experiment in Wyoming, USA. We measured soil inorganic N, plant and microbial N pool s...
|
PMID: 20487311
PDF is available here.
PDF is available here.
New Phytologist (187)2 2010
Abstract
We studied N cycling under ambient and elevated CO(2) concentrations (600 micromol mol(-1)), and ambient and elevated temperature (1.5 : 3.0 degrees C warmer day:night) in a full factorial semiarid grassland field experiment in Wyoming, USA. We measured soil inorganic N, plant and microbial N pool s...
|
PMID: 20487311
PDF is available here.
PDF is available here.
New Phytologist (187)2 2010
Abstract
We studied N cycling under ambient and elevated CO(2) concentrations (600 micromol mol(-1)), and ambient and elevated temperature (1.5 : 3.0 degrees C warmer day:night) in a full factorial semiarid grassland field experiment in Wyoming, USA. We measured soil inorganic N, plant and microbial N pool s...
|
PMID: 20487311
PDF is available here.
PDF is available here.
New Phytologist (187)2 2010
Abstract
We studied N cycling under ambient and elevated CO(2) concentrations (600 micromol mol(-1)), and ambient and elevated temperature (1.5 : 3.0 degrees C warmer day:night) in a full factorial semiarid grassland field experiment in Wyoming, USA. We measured soil inorganic N, plant and microbial N pool s...
|
PMID: 20487311
PDF is available here.
PDF is available here.
New Phytologist (187)2 2010
Abstract
We studied N cycling under ambient and elevated CO(2) concentrations (600 micromol mol(-1)), and ambient and elevated temperature (1.5 : 3.0 degrees C warmer day:night) in a full factorial semiarid grassland field experiment in Wyoming, USA. We measured soil inorganic N, plant and microbial N pool s...
|
PMID: 20487311
PDF is available here.
PDF is available here.
New Phytologist (187)2 2010
Abstract
We studied N cycling under ambient and elevated CO(2) concentrations (600 micromol mol(-1)), and ambient and elevated temperature (1.5 : 3.0 degrees C warmer day:night) in a full factorial semiarid grassland field experiment in Wyoming, USA. We measured soil inorganic N, plant and microbial N pool s...
|
PMID: 20487311
PDF is available here.
PDF is available here.
New Phytologist (187)2 2010
Abstract
We studied N cycling under ambient and elevated CO(2) concentrations (600 micromol mol(-1)), and ambient and elevated temperature (1.5 : 3.0 degrees C warmer day:night) in a full factorial semiarid grassland field experiment in Wyoming, USA. We measured soil inorganic N, plant and microbial N pool s...
|
PMID: 20487311
PDF is available here.
PDF is available here.
New Phytologist (187)2 2010
Abstract
We studied N cycling under ambient and elevated CO(2) concentrations (600 micromol mol(-1)), and ambient and elevated temperature (1.5 : 3.0 degrees C warmer day:night) in a full factorial semiarid grassland field experiment in Wyoming, USA. We measured soil inorganic N, plant and microbial N pool s...
|
PMID: 20487311
PDF is available here.
PDF is available here.
New Phytologist (187)2 2010
Abstract
We studied N cycling under ambient and elevated CO(2) concentrations (600 micromol mol(-1)), and ambient and elevated temperature (1.5 : 3.0 degrees C warmer day:night) in a full factorial semiarid grassland field experiment in Wyoming, USA. We measured soil inorganic N, plant and microbial N pool s...
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PMID: 20487311
PDF is available here.
PDF is available here.
Abstract
We have developed a rapid and cost-efficient strategy that combines cell-free (CF) protein synthesis, optimized combinatorial dual-isotope labeling for nearly instant resonance assignment, and fast acquisition of long-distance information using paramagnetic probes. Here we report three backbone stru...
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PMID: 20498088
PDF is available here.
PDF is available here.
Abstract
We used N-addition experiments to achieve N-enriched conditions in mixed-species, lowland and montane forests in Panama. Our objectives were to (1) assess changes in soil mineral N production (gross rates of N mineralization and nitrification) and retention (microbial immobilization and rapid reacti...
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PMID: 20583713
PDF is available here.
PDF is available here.
Abstract
We employed NMR spectroscopy to monitor the conformation of the SH3 domain from alpha-spectrin at sequential stages of elongation via in vivo ribosome-arrested (15)N,(13)C-labeled nascent polypeptides. These nascent chains exposed either the entire SH3 domain or C-terminally truncated segments there...
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PMID: 20439768
PDF is available here.
PDF is available here.
Abstract
We use a variety of methods to reconstruct bald eagle diets and local abundance of their potential prey on the Channel Islands from the late Pleistocene to the time when the last breeding pairs disappeared from the islands in the mid-20th century. Faunal and isotopic analysis of bald eagles shows th...
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PMID: 20439737
PDF is available here.
PDF is available here.
Abstract
We describe a pseudopotential E(relax) that permits direct use of (15)N relaxation rates, in the form of R(2)/R(1) ratios, as experimental restraints in structure calculations without requiring prior information to be extracted from a known molecular structure. The elements of the rotational diffusi...
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PMID: 20392103
PDF is available here.
PDF is available here.
Abstract
Spectra obtained by application of multidimensional Fourier Transformation (MFT) to sparsely sampled nD NMR signals are usually corrupted due to missing data. In the present paper this phenomenon is investigated on simulations and experiments. An effective iterative algorithm for artifact suppressio...
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PMID: 20372976
PDF is available here.
PDF is available here.
Narrow carbonyl resonances in proton-diluted proteins facilitate NMR assignments in the solid-state.
Abstract
HNCO/HNCACO type correlation experiments are an alternative for assignment of backbone resonances in extensively deuterated proteins in the solid-state, given the fact that line widths on the order of 14-17 Hz are achieved in the carbonyl dimension without the need of high power decoupling. The achi...
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PMID: 20232230
PDF is available here.
PDF is available here.
Abstract
An approach for generating efficient NR(vS, vk)(n) symmetry-based dual channel RF pulse schemes for gamma-encoded broadband (15)N-(13)C dipolar recoupling at high magic angle spinning frequencies is presented. The method involves the numerical optimisation of the RF phase-modulation profile of the b...
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PMID: 20306117
PDF is available here.
PDF is available here.
Abstract
Escherichia coli (E. coli) is a versatile organism for making nucleotides labeled with stable isotopes ((13)C, (15)N, and/or (2)H) for structural and molecular dynamics characterizations. Growth of a mutant E. coli strain deficient in the pentose phosphate pathway enzyme glucose-6-phosphate dehydrog...
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PMID: 20309608
PDF is available here.
PDF is available here.