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Phosphorus Isotopes (1):
Recent article
Phosphorus Isotopes
Biochimica et Biophysica Acta (1807)9 2011
Abstract
We assessed ΔG'(ATP) hydrolysis, cytosolic [ADP], and the rate of phosphocreatine recovery using Phosphorus Magnetic Resonance Spectroscopy in the calf muscle of a group of patients affected by glycogen myo-phosphorylase deficiency (McArdle disease). The goal was to ascertain whether and to what ex...
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PMID: 21722623
PDF is available here.
PDF is available here.
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Kidney International (78)10 2010
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Kidney International (78)10 2010
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Kidney International (78)10 2010
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Kidney International (78)10 2010
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Kidney International (78)10 2010
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Kidney International (78)10 2010
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Kidney International (76) 2010
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Kidney International (76) 2010
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Kidney International (76) 2010
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Kidney International (78)10 2010
Abstract
Kidney International (78)10 2010
Abstract
Kidney International (76) 2010
Abstract
Kidney International (76) 2010
Abstract
Kidney International (76) 2010
Abstract
Kidney International (76) 2010
Abstract
Kidney International (76) 2010
Abstract
Kidney International (78)10 2010
Abstract
Kidney International (78)10 2010
Abstract
Kidney International (78)10 2010
Abstract
Kidney International (78)10 2010
Abstract
Kidney International (78)10 2010
Abstract
Kidney International (78)10 2010
Abstract
Kidney International (78)10 2010
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Abstract
We investigated the relationship between high-energy phosphate (HEP) and cardiac function following CABG using cine magnetic resonance imaging (cine-MRI) and phosphorus-31 magnetic resonance spectroscopy ((31)P-MRS). A retrospective study was approved by the institutional review board. MRI and (31)P...
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PMID: 20444565
PDF is available here.
PDF is available here.
Abstract
We describe the recombinant production of the human Y(1) receptor from inclusion bodies of E. coli cultures. The in vitro refolding was carried out in the presence of lipids from bovine brain extracts. Y(1) receptors in brain lipids compete for cellular receptors in competitive binding experiments....
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PMID: 19689227
PDF is available here.
PDF is available here.
Abstract
The effects of dark-induced stress on the evolution of the soluble metabolites present in senescent soybean (Glycine max L.) nodules were analysed in vitro using (13)C- and (31)P-NMR spectroscopy. Sucrose and trehalose were the predominant soluble storage carbons. During dark-induced stress, a decli...
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PMID: 20358222
PDF is available here.
PDF is available here.
Abstract
The quantity and composition of phospholipids in full-fat soybean flour, flakes, and extruded flakes and in the cream fraction recovered after aqueous extraction (AEP) and enzyme-assisted aqueous extraction (EAEP) of these substrates were studied with (31)P NMR. Extruded flakes had significantly mor...
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PMID: 20329795
PDF is available here.
PDF is available here.
Abstract
We have reported two-dimensional HPP-COSY spectroscopy techniques to identify diphosphate-containing metabolic intermediates at submillimolar concentrations in the methylerythritol phosphate (MEP) isoprenoid biosynthetic pathway. (1) In this work, we explore the scope of HPP-COSY-based techniques to...
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PMID: 20408590
PDF is available here.
PDF is available here.
Abstract
We report here the (1)H, (15)N, (13)C and partial (31)P chemical shift assignments for the minimal functional 27nt neomycin sensing riboswitch RNA in complex with the 4,5-linked neomycin analog ribostamycin and the 4,6-linked aminoglycoside tobramycin....
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PMID: 20306311
PDF is available here.
PDF is available here.
Abstract
To evaluate the changes of phosphorus metabolites in leukemic infiltration of liver (LIL) with two-dimensional chemical shift imaging(2D CSI)(31)phosphorus MR spectroscopy ((31)P MRS).
Fifteen patients with LIL and 12 healthy subjects (control group) were scanned with liver 2D CSI(31)P MRS by a 1.5T...
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PMID: 20387242
PDF is available here.
PDF is available here.
Abstract
To investigate the biochemical metabolic changes detected by phosphorus-31 MR spectroscopy ((31)P MRS) with pathologic changes in the liver of fasting rabbits.
A total of 22 rabbits were under the starvation up to death to establish animal models. Hepatic (31)P MRS was performed in different period...
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PMID: 20387241
PDF is available here.
PDF is available here.
Applied Spectroscopy (64)3 2010
Abstract
Solid-state nuclear magnetic resonance (NMR) spectroscopic studies are reported for the interactions of probe molecules with respirable silicon-containing dusts as experimental evidence complementing computational studies reported by Snyder and Madura recently in J. Phys. Chem. B 112, 7095 (2008). T...
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PMID: 20223070
PDF is available here.
PDF is available here.
Abstract
We use multiple NMR techniques to characterize interaction of spermidine with adenosine 5'-triphosphate (ATP). Two-dimensional (1)H-(15)N spectra obtained from gs-HMBC experiments at varied pH show significant shift of N-1 peak around pH 2.0-7.0 range, suggesting that spermidine binds to N-1 site of...
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PMID: 19960498
PDF is available here.
PDF is available here.
Abstract
5-Diisopropyloxy-phosphoryl-5-methyl-1-pyrroline-N-oxide (DIPPMPO) was used to trap a variety of free radicals and the stable compounds generated by the natural decomposition of the initially formed spin adducts were characterized by (31)P nuclear magnetic resonance (NMR) and mass spectrometry. Init...
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PMID: 20212327
PDF is available here.
PDF is available here.
Abstract
Pretreatment with chemicals such as ethylenediaminetetraacetic acid (EDTA) is often used to improve the analysis of sediment P with solution P-31 nuclear magnetic resonance spectroscopy (35P-NMR), but there is a lack of a comprehensive assessment of the methodology. In this study, the effects of EDT...
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PMID: 21043272
PDF is available here.
PDF is available here.
Abstract
In many regions, conservation tillage has replaced conventional tilling practices to reduce soil erosion, improve water conservation, and increase soil organic matter. However, tillage can have marked effects on soil properties, specifically nutrient redistribution or stratification in the soil prof...
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PMID: 21043270
PDF is available here.
PDF is available here.
Abstract
Our aim was to establish cardiac (31)P MR spectroscopy during leg exercise at 3T. The increased field strength should provide a higher signal to noise ratio than at lower field strengths. Furthermore, relatively high temporal resolution at a sufficiently fine spatial resolution should be feasible. (...
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PMID: 19697152
PDF is available here.
PDF is available here.
Abstract
A perfusion small-scale bioreactor allowing on-line monitoring of the cell energetic state was developed for free-suspension mammalian cells. The bioreactor was designed to perform in vivo nuclear magnetic resonance (NMR) spectroscopy, which is a noninvasive and nondestructive method...
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PMID: 19938168
PDF is available here.
PDF is available here.
Abstract
We present a novel method for up-concentration and identification of nrP in lake water: nrP is precipitated with poly aluminum chloride and the precipitate is subsequently recovered and dissolved by NaOH. Additional up-concentration by rotary evaporation increases P concentrations up to 5500 times....
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PMID: 19708371
PDF is available here.
PDF is available here.
Biophysical Journal (97)1 2009
Abstract
We use the temperature dependence of 31P R1 at low field (0.03-0.08 T), which reflects this correlation time, to explore the energy barriers associated with this motion. Most phospholipids exhibit a similar energy barrier of 13.2 +/- 1.9 kJ/mol at temperatures above that associated with their gel-to...
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PMID: 19580751
PDF is available here.
PDF is available here.
Biophysical Journal (96)9 2009
Abstract
We used (2)H NMR spectroscopy to investigate the structure and dynamics of the myristoyl chain of myr-Src(2-19), and compare them with the hydrocarbon chains of the surrounding phospholipids in bilayers of varying surface potentials and chain lengths. The myristoyl chain of Src was well inserted in...
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PMID: 19413971
PDF is available here.
PDF is available here.
Biophysical Journal (96)9 2009
Abstract
SP-B(CTERM), a cationic, helical peptide based on the essential lung surfactant protein B (SP-B), retains a significant fraction of the function of the full-length protein. Solid-state (2)H- and (31)P-NMR were used to examine the effects of SP-B(CTERM) on mechanically oriented lipid bilayer samples....
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PMID: 19413982
PDF is available here.
PDF is available here.
NMR in Biomedicine (22)2 2009
Abstract
Patients with extrahepatic biliary obstruction present with impairment of the normal bile flow, with jaundice and cholangitis as common complications. (1)H and (31)P NMR quantitative analysis of bile specimens from patients with extrahepatic biliary obstruction (n = 80) (with/without jaundice and ch...
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PMID: 18837066
PDF is available here.
PDF is available here.
NMR in Biomedicine (22)2 2009
Abstract
The aim of this study was to determine the metabolic changes in the transition from pre-invasive to invasive cervical cancer using high-resolution magic angle spinning (HR-MAS) MRS. Biopsy specimens were obtained from women with histologically normal cervix (n = 5), cervical intraepithelial neoplasi...
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PMID: 18833545
PDF is available here.
PDF is available here.
Environmental Pollution (157)1 2009
Abstract
Phosphorus (P) pollution in the sediments of seven artificial landscape lakes was studied via fractionation and phosphorus-31 nuclear magnetic resonance ((31)P NMR) spectroscopy. The lake sediments accumulated significant amounts of P from supplementation with reclaimed water and from runoff from th...
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PMID: 18804318
PDF is available here.
PDF is available here.
Abstract
Effective myocardial oxygen supply should not be compromised during cardiac surgery as it is essential to avoid circulatory and cardiac dysfunction. Local measurement of myocardial oxygen partial pressure (pO2) was therefore introduced into the operative monitoring of myocardial ischemia. The aim of...
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PMID: 19807283
PDF is available here.
PDF is available here.
Abstract
Information on the chemical composition of sediment phosphorus (P) is fundamental to understanding P dynamics and eutrophication in lake ecosystems. In this study, the surface (10 cm) sediments were collected from seven lakes representing two contrasting ecological areas in China: the middle and low...
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PMID: 19141826
PDF is available here.
PDF is available here.
Abstract
We measured the incidence of all P compounds using solution 31P nuclear magnetic resonance (NMR) spectroscopy and extracts, relating them to relevant geochemical properties. The contribution of phytate to the overall pool of P declined from around 50% in manures to between 2 and 13% in down-gradient...
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PMID: 19141802
PDF is available here.
PDF is available here.
Journal of Neurochemistry (108)2 2009
Abstract
We demonstrate that NorSAL reduces cell viability and induces apoptosis via cytochrome c release and caspase 3 activation in SH-SY5Y human neuroblastoma cells. Cytochrome c release, caspase 3 activation, and apoptosis induction were all inhibited by the antioxidant N-acetylcysteine. Thus, reactive o...
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PMID: 19012744
PDF is available here.
PDF is available here.
Biochemistry (Washington) (47)52 2008
Abstract
Lumazine synthase catalyzes the reaction of 5-amino-6-D-ribitylamino-2,4(1H,3H)-pyrimidinedione(1) with (S)-3,4-dihydroxybutanone 4-phosphate (2) to afford 6,7-dimethyl-8-D-ribityllumazine(3), the immediate biosynthetic precursor of riboflavin. The overall reaction implies a series of intermediates...
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PMID: 19117095
PDF is available here.
PDF is available here.
Abstract
NaF caused RBC-shape evolution from discocytes, through various forms of echinocytes, to spherocytes, over approximately 6 h and approximately 10 h at 37 degrees C and 25 degrees C, respectively. ATP declined to approximately 0.5 its normal concentration before the first stage of discocyte transform...
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PMID: 19025949
PDF is available here.
PDF is available here.
Abstract
Biochimica et Biophysica Acta (1778)11 2008
Abstract
We previously showed that the ability of a fluoroquinolone antibiotic, ciprofloxacin, to induce disorder and modify the orientation of the acyl chains is related to its propensity to be expelled from a monolayer upon compression [1]. Here, we compared the binding of ciprofloxacin on DPPC and DPPG li...
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PMID: 18809375
PDF is available here.
PDF is available here.
Abstract
We show that chemical shift parameters can be determined with high accuracy by analysing the correlation pattern from a 2D experiment which correlates a powder pattern in the indirect dimension with a sideband pattern in the direct dimension. The chemical shift parameters so determined have greater...
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PMID: 18666220
PDF is available here.
PDF is available here.
Biochemical Journal (414)3 2008
Abstract
We used (31)P MRS (magnetic resonance spectroscopy) measurements of energetic intermediates [ATP, P(i) and PCr (phosphocreatine)] in combination with the analytical tools of metabolic control analysis to study in vivo energy metabolism in the contracting skeletal muscle of anaesthetized rats over a...
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PMID: 18498244
PDF is available here.
PDF is available here.
Abstract
Proton-detected NMR diffusion and (31)P NMR chemical shifts/bandwidths measurements were used to investigate a series of liposomal formulations where size and PEGylation extent need to be controlled for ultrasound mediated drug release. The width of the (31)P line is sensitive to aggregate size and...
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PMID: 18589432
PDF is available here.
PDF is available here.
Journal of Physical Chemistry B (112)36 2008
Abstract
We incorporated a well-studied peptide, protegrin-1 (PG-1), a beta-sheet AMP, to investigate AMP-induced dynamic supramolecular lipid assemblies at different peptide concentrations and membrane compositions. Anisotropic NMR line shapes specifying toroidal pores and thinned membranes, which are forme...
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PMID: 18700738
PDF is available here.
PDF is available here.
Abstract
The main aim of this study was to examine the hypothesis that creatine (Cr) feeding enhances myocellular glycogen storage in humans undergoing carbohydrate loading. Twenty trained male subjects were randomly assigned to have their diets supplemented daily with 252 g of glucose polymer (GP) and eithe...
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PMID: 19244932
PDF is available here.
PDF is available here.
Magnesium Research (21)3 2008
Abstract
Phosphorus magnetic resonance spectroscopy offers a unique opportunity to measure in vivo the free cytosolic magnesium [Mg2+] of different tissues. In particular, this technique has been employed in human brain and in skeletal muscle providing new hints on Mg2+ homeostasis and on its involvement in...
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PMID: 19009818
PDF is available here.
PDF is available here.