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Analytic Sample Preparation Methods (0):
Recent article
Analytic Sample Preparation Methods
Abstract
With the advent of "-omics" technologies, there has been an explosion of data generation in the field of toxicology, as well as in many others. As new candidate biomarkers of toxicity are being regularly discovered, the next challenge is to validate these observations in a targeted manner. Tradition...
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PMID: 20972768
PDF is available here.
PDF is available here.
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Abstract
Metabonomics, also often referred to as "metabolomics" or "metabolic profiling," is the systematic profiling of metabolites in bio-fluids or tissues of organisms and their temporal changes. In the last decade, metabonomics has become increasingly popular in drug development, molecular medicine, and...
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PMID: 20972767
PDF is available here.
PDF is available here.
Abstract
Fast protein liquid chromatography (FPLC) is a form of high-performance chromatography that takes advantage of high resolution made possible by small-diameter stationary phases. It was originally developed for proteins and features high loading capacity, biocompatible aqueous buffer systems, fast fl...
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PMID: 20978981
PDF is available here.
PDF is available here.
Abstract
We show the accurate proteomic measurements in thylakoid membranes. The chlorophyll-containing membrane protein complexes were precipitated using chloroform/methanol solution. These complexes were separated using two-dimensional gel electrophoresis, and the resolved spots were exercised from the gel...
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PMID: 20960129
PDF is available here.
PDF is available here.
Abstract
We describe simple protocols for protein digestion and the analysis of digested peptides produced both by one-dimensional reversed-phase liquid chromatography and two-dimensional liquid chromatography (i.e. strong cation exchange chromatography followed by reversed-phase chromatography), coupled wit...
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PMID: 20978984
PDF is available here.
PDF is available here.
Abstract
Major difficulties in phosphoprotein analysis relate to the presence of a huge number of nonphosphorylated proteins and to the wide concentration dynamic range among them. In order to overcome the analysis complexity, specific clean-up and highly efficient enrichment procedures are mandatory prior t...
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PMID: 20978974
PDF is available here.
PDF is available here.
Abstract
Shotgun proteomics originated as a strategy to identify proteins in complex protein mixtures, but it is also possible to obtain information on relative quantitation with some adjustments to the procedure. After protein digestion, the resulting peptide mixture is labelled with isobaric tags. Then, la...
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PMID: 20978983
PDF is available here.
PDF is available here.
Abstract
Shotgun proteomics is a rapid and near universal strategy to identify proteins in complex protein mixtures. After protein digestion, the resulting peptide mixture is submitted to two orthogonal techniques: peptides are first separated according to their isoelectric point (pI) by isoelectric focusing...
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PMID: 20978982
PDF is available here.
PDF is available here.
Abstract
Expression of recombinant proteins in the baculovirus/insect cell expression system is employed because it enables post-translational protein modification and high yields of recombinant protein. The system is capable of facilitating the functional expression of many proteins - either secreted or int...
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PMID: 20978972
PDF is available here.
PDF is available here.
Abstract
A rapid, simple and sensitive multi-residue method was developed and validated for the simultaneous quantification and confirmation of 69 pesticides in fruit and vegetables using liquid chromatography-tandem mass spectrometry (LC-MS/MS). The samples were extracted following the quick, easy, cheap, e...
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PMID: 20730643
PDF is available here.
PDF is available here.
Abstract
The aims of the study were to obtain information about the occurrence of ochratoxin A (OTA) and citrinin (CIT) in cereals harvested in the Czech Republic and to compare two analytical procedures for detecting OTA. A total of 34 cereal samples, including two matrix reference materials (R-Biopharm, Ge...
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PMID: 20835934
PDF is available here.
PDF is available here.
New Microbiologica (33)4 2010
Abstract
Our results have implications for studies concentrating on salivary microbiome and its role in the pathogenesis of oral diseases....
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PMID: 21213596
PDF is available here.
PDF is available here.
Abstract
We obtained spatial- and concentration-dependent distributions of seven lens sphingomyelins (SM) and two ceramide-1-phosphates (CerP), which are important lipid second messengers. Glycosylated sphingolipids or sphingolipid breakdown products were not observed. Owing to ultra high resolution MS, all...
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PMID: 20388918
PDF is available here.
PDF is available here.
Bioanalysis (2)8 2010
Abstract
We have evaluated three distinct sample preparation procedures of DBS samples for LC-MS/MS assay development.
A new term 'elution efficiency' is introduced to evaluate the effectiveness of eluting compounds from the DBS cards into the liquid phase. Three different types of DBS cards...
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PMID: 21083341
PDF is available here.
PDF is available here.
Abstract
In most diseases, the clinical need for serum/plasma markers has never been so crucial, not only for diagnosis, but also for the selection of the most efficient therapies, as well as exclusion of ineffective or toxic treatment. Due to the high sample complexity, prefractionation is e...
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PMID: 20685463
PDF is available here.
PDF is available here.
Lab On a Chip (10)12 2010
Abstract
A new method for in-gel sample processing and tryptic digestion of proteins is described. Sample preparation, rehydration, in situ digestion and peptide extraction from gel slices are dramatically accelerated by treating the gel slice with surface acoustic waves (SAWs). Only 30 minutes total workflo...
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PMID: 20517556
PDF is available here.
PDF is available here.
Biomedical Microdevices (12)3 2010
Abstract
We also tested this on-chip droplet generation and manipulation platform using a two-step thermal cycled bioreaction: nested TaqMan PCR. With the same concentration of template DNA, the two-step reaction in a well-mixed merged droplet shows a cycle threshold of approximately 6 cycles earlier than th...
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PMID: 20204702
PDF is available here.
PDF is available here.
Abstract
We review the techniques themselves for qualitative and quantitative metalloproteomics, and provide evidence in the form of several example of different kinds of expertise working together to produce coherent and effective results in the area....
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PMID: 20536316
PDF is available here.
PDF is available here.
Bioanalysis (2)6 2010
Abstract
A novel approach for regulated bioanalytical sample preparation has been developed to combine multiple types of extraction techniques into one integrated and automated sample-preparation suite that pairs a graphical user interface with the Hamilton Microlab(®) STAR robotic liquid ha...
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PMID: 21083206
PDF is available here.
PDF is available here.
Chemical Reviews (110)5 2010
Abstract
Abstract
A new pyrazole derivative 1-phenyl-3-methylthio-4-cyano-5-acrylicacidcarbamoyl-pyrazole (PMTCAACP) was synthesized and chosen as a complexing monomer for the preparation of surface-grafted ion-imprinted polymers for selective solid-phase extraction of thorium(IV). The silica gel, modified with malei...
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PMID: 20188883
PDF is available here.
PDF is available here.
Abstract
A novel method of ultrasonic probe-assisted ionic liquid dispersive liquid-liquid microextraction combined with electrothermal atomic absorption spectrometry (ETAAS) was developed for the determination of chromium(VI) species in water samples. In this procedure, the hydrophobic chelate of chromium(V...
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PMID: 20188905
PDF is available here.
PDF is available here.
Abstract
Arsenic (As) speciation in muscle tissues of freshwater fish was investigated with special emphasis on extraction yields and mass balances. For the quantification of water-soluble As compounds, samples were extracted using a 1:1 (v/v) methanol/water mixture. Various extraction parameters, such as ex...
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PMID: 20188911
PDF is available here.
PDF is available here.
Abstract
A simple and efficient method for the determination of ultra-trace amounts of inorganic mercury (iHg) and methylmercury (MeHg) in waters and fish tissues was developed using a micro-column filled with polyaniline (PANI) coupled online to flow injection-chemical vapour generation-inductively coupled...
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PMID: 20188947
PDF is available here.
PDF is available here.
Abstract
A mixed micelle-cloud point extraction (MM-CPE) has been developed for the analysis of penicillin antibiotics (ampicillin, penicillin G, oxacillin, and cloxacillin) in milk samples using Triton X-114 (TX-114) and cethyl trimethylammonium bromide (CTAB) as the mixed micellar extractant. The parameter...
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PMID: 20188951
PDF is available here.
PDF is available here.
Abstract
A new method based on a microcolumn packed with ionic liquid-modified silica combined with flame atomic absorption spectrometry has been developed for the determination of trace amount of cadmium (Cd) in environmental samples. The ionic liquid-modified silica sorbent was prepared by the surface of c...
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PMID: 20188980
PDF is available here.
PDF is available here.
Abstract
A method based on coupling of cloud point extraction (CPE) with high performance liquid chromatography separation and ultraviolet detection was developed for determination of xanthohumol in beer. The nonionic surfactant Triton X-114 was chosen as the extraction medium. The parameters affecting the C...
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PMID: 20188983
PDF is available here.
PDF is available here.
Journal of Biochemistry (147)4 2010
Abstract
Rare sugars are defined as monosaccharides with extremely low natural abundances. Their natural distributions and biological functions remain to be clarified. To establish a robust analytical system that can separate, identify and quantify rare sugars, 12 d-hexoses-including five rare aldohexoses an...
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PMID: 19948774
PDF is available here.
PDF is available here.
Methods in Molecular Biology (632)632 2010
Abstract
We uncover. Understanding the mechanisms of these aberrations to guide additional testing of the parents and genetic counseling of the patients and their families requires the expertise of individuals who are well-versed in meiotic mechanisms and chromosomal structures that may lead to these abnorma...
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PMID: 20217575
PDF is available here.
PDF is available here.
Methods in Molecular Biology (627)627 2010
Abstract
We present protocols for capturing, washing and eluting proteins from Biacore instruments as well as for robust sample preparation for sensitive mass spectrometric identification....
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PMID: 20217629
PDF is available here.
PDF is available here.
Methods in Molecular Biology (627)627 2010
Abstract
We obtained best and reproducible results when the lectin was immobilized and its ligands were used as soluble analytes. With heterogeneous glycoconjugates such as neoglycoproteins (which are heterogeneous in terms of nature, number, and position of sugar residues) or a mixture of oligosaccharides,...
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PMID: 20217620
PDF is available here.
PDF is available here.
Abstract
We confirm these findings in regard to floral pollen, using pollen collected directly from flowers of the known PA plants Senecio jacobaea, S. vernalis, Echium vulgare and pollinia of Phalaenopsis hybrids, and we extend analyses of 1,2-unsaturated PAs and 1,2-unsaturated PANOs to include bee-pollen...
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PMID: 20013884
PDF is available here.
PDF is available here.
Abstract
We confirm these findings in regard to floral pollen, using pollen collected directly from flowers of the known PA plants Senecio jacobaea, S. vernalis, Echium vulgare and pollinia of Phalaenopsis hybrids, and we extend analyses of 1,2-unsaturated PAs and 1,2-unsaturated PANOs to include bee-pollen...
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PMID: 20013884
PDF is available here.
PDF is available here.
Abstract
A method for determining all of the six natural vitamin B(6) compounds and pyridoxine-beta-glucoside in urine from humans consuming their usual diet was developed. These compounds were specifically converted with 5 enzymes into a high fluorescent 4-pyridoxolactone which was supersensitively determin...
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PMID: 20651455
PDF is available here.
PDF is available here.
Abstract
We describe procedures for reliable and accurate quantification of retinoids, including detailed descriptions for handling retinoids, preparing standard solutions, collecting samples and harvesting tissues, extracting samples, resolving isomers, and detecting with high sensitivity. Sample-specific s...
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PMID: 20552420
PDF is available here.
PDF is available here.
Analytical Biochemistry (398)2 2009
Abstract
Quantification of heparosan produced from the E. coli K5 strain typically relies on chromatography following extensive purification. Such purification steps are both time-consuming and can result in sample loss and thus may not accurately reflect the amount of heparosan in the origin...
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PMID: 20018166
PDF is available here.
PDF is available here.
Abstract
A novel sample preparation technique for the measurement of anions in particulate matter by ion chromatography is presented. The technique uses the eluant from the ion chromatography analysis stage to perform the extraction of the anions from the particulate matter. The demonstrated advantages of th...
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PMID: 19836590
PDF is available here.
PDF is available here.
Abstract
Partitioned dispersive liquid-liquid microextraction (PDLLME), using THF as the dispersive solvent and dichloromethane as the extraction solvent, was utilized to isolate and concentrate phenylurea herbicides (PUHs) from aqueous samples. In PDLLME, a dispersive solvent should be able to partition in...
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PMID: 19836510
PDF is available here.
PDF is available here.
Abstract
A method for the extraction of triethyl lead (TEL(+)), trimethyl lead (TML(+)), and Pb(2+) from sand was developed using supercritical modified CO(2)-CH(3)OH extraction and in situ complexation with sodium diethyldithiocarbamate (NaDDTC) using a 2(5) factorial exploratory design is described. The sc...
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PMID: 19836512
PDF is available here.
PDF is available here.
Audio, Transactions of the IRE Professional Group on (Chapter 11) 2009
Abstract
Amino acid analysis is used to determine the amino acid content of amino acid-, peptide- and protein-containing samples. With minor exceptions, proteins are long linear polymers of amino acids connected to each other via peptide bonds. The first step of amino acid analysis involves hydrolyzing these...
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PMID: 19937719
PDF is available here.
PDF is available here.
Nature Methods (6)11 2009
Abstract
Cytometry Part A (75A)10 2009
Abstract
We discuss the recent advances and applications that fulfill the criteria for clinical proteomics with the focus on cellular proteomics (cytoproteomics) as related to preanalytical and analytical standardization and to quality control measures required for effective implementation of these technolog...
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PMID: 19739086
PDF is available here.
PDF is available here.
Abstract
A simple and stability-indicating high performance liquid chromatographic method was developed and validated for the determination of miconazole nitrate in bulk and cream preparations. The extraction step for cream samples consisted in a warming, cooling and centrifugation procedure that assures the...
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PMID: 19576442
PDF is available here.
PDF is available here.
Abstract
A flow injection online speciation procedure by using micro-column packed with Cu(II) loaded nanometer-sized Al(2)O(3) coupled to inductively coupled plasma mass spectrometry (ICP-MS) for the separation and determination of selenomethionine (SeMet) and selenocystine (SeCys(2)) has been developed. Th...
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PMID: 19576438
PDF is available here.
PDF is available here.
Abstract
OBJECTIVE: To study pre-treatment in determining total polysaccharide in flos Chrysanthemi Indici. METHOD: The factors including the extraction temperature, extraction time, ratio of material to liquid were studied. The best extraction condition was found through the response surface design. RESULT:...
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PMID: 19873776
PDF is available here.
PDF is available here.
Analyst (Cambridge UK) (134)7 2009
Abstract
A fast, selective and sensitive method is here proposed for the analysis of female steroid hormones as conjugated forms (mainly, glucuronides and sulfates). The method has been applied to female urine samples to assess the metabolism of these compounds. The method implements an enzymatic hydrolysis...
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PMID: 19562210
PDF is available here.
PDF is available here.
Journal of Chromatography B (877)20-21 2009
Abstract
A method using high-performance liquid chromatography coupled with tandem mass spectrometry (HPLC-MS/MS) was developed to screen and confirm residues of multi-class veterinary drugs in animal tissues (porcine kidney, liver, muscle; bovine muscle). Thirty target drugs (19 beta-blocker...
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PMID: 19493712
PDF is available here.
PDF is available here.
Journal of Chromatography B (877)20-21 2009
Abstract
The applicability of hollow fiber liquid phase microextraction (HF-LPME) for extraction and preconcentration of trace amounts of pioglitazone (PGL) as an anti-diabetic drug in biological fluids, prior to determination by high-performance liquid chromatography (HPLC), was evaluated. I...
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PMID: 19501030
PDF is available here.
PDF is available here.
Bioanalysis (1)4 2009
Abstract
The plasma proteome offers a wealth of opportunity to develop protein-based assays for diagnostic, prognostic and predictive biomarkers. Nonetheless, the unique properties of plasma, with its high dynamic range of protein concentrations and the vast complexity of protein species, pre...
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PMID: 21083142
PDF is available here.
PDF is available here.
Analytical Biochemistry (393)2 2009
Abstract
We report the first application of this technique to the study of an enzyme-catalyzed reaction, the bioconversion of ferulic acid to vanillin in cultures of Streptomyces setonii. We were able to show that the NAQ-NMR methodology is sufficiently precise and robust to measure the isotope shifts in the...
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PMID: 19563771
PDF is available here.
PDF is available here.
Analytical Biochemistry (393)2 2009
Abstract
A simple method for the determination of oxysterols was developed by semi-micro high-performance liquid chromatography with electrochemical detection (semi-micro HPLC-ECD). Semi-micro HPLC-ECD was established using a C30 microbore column, acetonitrile containing 50mmol/L LiClO(4) as...
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PMID: 19563770
PDF is available here.
PDF is available here.
Abstract
A novel solid phase extraction technique was developed for the determination of trace lead, cadmium and copper in environmental water samples based on separation and preconcentration with a microcolumn packed with crosslinked carboxymethyl konjac glucomannan (CCMKGM)prior to its determination by gra...
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PMID: 19650505
PDF is available here.
PDF is available here.
Abstract
The following study investigates the preparation of human blood plasma for metabolomic profiling analysis by ultrahigh performance liquid chromatography coupled to time-of-flight mass spectrometry (UPLC/TOFMS) in a novel two-step design study. Four different organic solvents (acetonitrile, acetone,...
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PMID: 19323527
PDF is available here.
PDF is available here.
Abstract
Analysis of scattered light can achieve high spatial resolution without labeling or sample preparation.
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PMID: 19402719
PDF is available here.
PDF is available here.
Abstract
An automated sample introduction system, utilizing a demountable direct injection high-efficiency nebulizer (d-DIHEN), is successfully incorporated for the first time with an inductively coupled plasma optical emission spectrometer (ICP-OES) for the measurement of the phosphorus content in acid-dige...
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PMID: 19344148
PDF is available here.
PDF is available here.
Abstract
A novel solid-phase preconcentration method is reported, using in-house gold-coated silica adsorbent packed in a microcolumn, for the determination of dissolved mercury in natural waters by atomic fluorescence spectrometry (AFS). The adsorbent was prepared by chemical reduction of a Au(III) solution...
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PMID: 19334705
PDF is available here.
PDF is available here.
Nature Methods (6)5 2009
Abstract
We describe a method, filter-aided sample preparation (FASP), which combines the advantages of in-gel and in-solution digestion for mass spectrometry-based proteomics. We completely solubilized the proteome in sodium dodecyl sulfate, which we then exchanged by urea on a standard filtration device. P...
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PMID: 19377485
PDF is available here.
PDF is available here.
Bioanalysis (1)1 2009
Abstract
'Omics' is a general term for a broad discipline of science and engineering concerned with analyzing the interactions of biological molecular components in various 'omes'. These include genome, proteome, metabolome, expressome and interactome. 'Ome' and 'omics' are very convenient ha...
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PMID: 21083194
PDF is available here.
PDF is available here.
Bioanalysis (1)1 2009
Abstract
The determination of chiral drugs and their metabolites in biological samples is key to gaining a full understanding of enantioselective drug action and disposition, as well as establishing the advantages of using racemate or isolated enantiomers. In this review, methods published in...
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PMID: 21083198
PDF is available here.
PDF is available here.
Abstract
These results validate the use of immobilized PPV targets and spot real-time RT-PCR as screening method for largescale analyses....
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PMID: 19440977
PDF is available here.
PDF is available here.