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Autoanalysis (0):
Recent article
Autoanalysis
Abstract
This method can be used to optimize thresholds for flag alerts on automated cell counters of any type and to improve the overall positive predictive value of the flagging system at the expense of a reduction in the negative predictive value. A reduced manual review rate helps to focus resources on d...
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PMID: 20923310
PDF is available here.
PDF is available here.
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Abstract
Abstract
The results obtained in this study strongly support the hypothesis that Test 1 measures only the RBC aggregation process and does not cover any of the indices directly linked to the sedimentation process as determined by the Westergren method....
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PMID: 19929267
PDF is available here.
PDF is available here.
Clinical laboratory (56)5-6 2010
Abstract
Our results suggest that the ISF scoring system of systemic function can be used to objectively evaluate the systemic function in individuals with different health statuses....
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PMID: 20575470
PDF is available here.
PDF is available here.
Abstract
Reference intervals for coagulation parameters have been rarely determined in dogs for the STA Compact automated coagulation analyzer, so it is the aim of the current study to validate assays and establish reference ranges for its use in canine specimens. Coagulation parameters were assessed in 56 h...
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PMID: 19901280
PDF is available here.
PDF is available here.
Abstract
Abstract
Abstract
We compared the IRIS iQ200 Body Fluids Module (Iris Diagnostics, Chatsworth, CA) and the Beckman-Coulter LH750 (Beckman-Coulter, Brea, CA) with manual counts and evaluated the impact of automation on the laboratory if clinically acceptable performance was to be maintained. Automated counts were comp...
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PMID: 19095566
PDF is available here.
PDF is available here.
Abstract
We routinely analyzed 99 peripheral blood smears, included 9 therapeutic cytopenias, stained with May-Grünwald-Giemsa. DM8/96TM is highly efficient in the cellular identification with great security of identity management and timesaving (30% faster than manual microscopy). Major innovations are t...
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PMID: 19654081
PDF is available here.
PDF is available here.
Abstract
Abstract
We have evaluated the analytical and clinical performances of the anti-CCP assay on the Elecsys analyzer (Roche Diagnostics). Two plasma pools (target values: 17.2 and 363.0 U/mL) and two quality controls (target values: 24.5 and 157.0 U/mL) were tested; we also analyzed three hundred plasma samples...
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PMID: 19654079
PDF is available here.
PDF is available here.
Journal of Food Protection (71)12 2008
Abstract
A two-stage study compared the BAX system PCR method with the reference culture method used by the Brazilian Ministry of Agriculture and Food Supply for the detection of Salmonella in food, water, and environmental samples. In stage 1, fish matrix samples (n = 258) were spiked at several levels with...
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PMID: 19244896
PDF is available here.
PDF is available here.
Clinical Chemistry (54)12 2008
Abstract
The Elecsys concentrations of 25-OH-D(3) were in good overall agreement with those determined with LC-MS/MS and RIA. However, large between-method variation was observed in individual patient samples. Use of serum rather than plasma is preferred owing to the higher results observed with plasma sampl...
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PMID: 18927245
PDF is available here.
PDF is available here.
Medical Laboratory Observer (40)10 2008
Abstract
Medical Laboratory Observer (40)10 2008
Abstract
Abstract
These results have also confirmed the great importance of Warburg's model for determining the electrical parameters of the electrode-electrolyte interface, and have shown that the measurement of intestinal mucosa electrical parameters can be achieved with a digital correction. Finally, the results s...
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PMID: 19088943
PDF is available here.
PDF is available here.
Abstract
Analysis of the training set data revealed that the ANC from test results flagged with the 'WBC abnormal scattergram' alarm was often invalid. After removal of these test results, the correlation between automated flagged and manual results was excellent (r = 0.95-0.99, P < 0.0001). The validity of...
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PMID: 18462258
PDF is available here.
PDF is available here.
Fertility and Sterility (90)2 2008
Abstract
OBJECTIVE: To determine the precision and accuracy of an automated cell counting system when applied to counting aneuploidies in sperm samples. DESIGN: Prospective pilot study. SETTING: Andrology clinic and research laboratory in a university teaching hospital. PATIENT(S): Ten anonymous sperm donors...
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PMID: 17936284
PDF is available here.
PDF is available here.
Abstract
The phosphate concentration in corticoid-phosphate formulations varies greatly, and is mainly determined by the chosen buffer. The prednisolone acetate preparations showed physiological phosphate concentrations. For a treatment on a damaged corneal surface, preparations with physiological phosphate...
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PMID: 18357463
PDF is available here.
PDF is available here.
Abstract
We have developed and evaluated a new staining method for use in electrophoretic analysis of alpha-amylase (AMY, EC3. 2. 1. 1) isoenzymes in serum. Our method based on the colorimetric determination of formazane dye formed by enzyme-coupled reactions and maltopentaose was used as a substrate. We hav...
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PMID: 18709986
PDF is available here.
PDF is available here.
Abstract
A flow system designed with solenoid micro-pumps is proposed for the determination of paraquat in natural waters. The procedure involves the reaction of paraquat with dehydroascorbic acid followed by spectrophotometric measurements. The proposed procedure minimizes the main drawbacks related to the...
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PMID: 18585227
PDF is available here.
PDF is available here.
Abstract
A high sensitive portable biosensor system capable of determining the presence of neurotoxic agents in water has been developed. The system consists of (i) a screen-printed electrode with acetylcholinesterase (AChE) immobilized on it, (ii) a self-developed portable potentiostat with an analog to dig...
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PMID: 18585203
PDF is available here.
PDF is available here.
Lab On a Chip (8)6 2008
Abstract
We present a review of microscale assays prone to evaporation and methods to make them more robust. Applications for these assays span from combinatorial chemistry to cell-biology where the stability of concentrations and osmolarity can be critical. A dimensionless evaporation number Ev is presented...
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PMID: 18497901
PDF is available here.
PDF is available here.
Abstract
We examined intermethod differences in total PSA (tPSA) and free PSA (fPSA) measurements. We tested 157 samples with tPSA concentrations of 2 to 10 ng/mL (2-10 microg/L) using 6 PSA/fPSA method pairs and 1 tPSA method: ADVIA Centaur (complexed and total; Siemens Diagnostics, Tarrytown, NY), ARCHITEC...
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PMID: 18480013
PDF is available here.
PDF is available here.
Abstract
Biogas is produced by biological processes under anaerobic conditions and may contain up to 20,000 ppm(v) hydrogen sulfide (H(2)S), a corrosive substance that attacks power engines and can affect the health of the industrial staff. H(2)S must be removed from the biogas, especially in co-generation f...
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PMID: 18297275
PDF is available here.
PDF is available here.
Theriogenology (69)9 2008
Assessment of motility of ejaculated, liquid-stored boar spermatozoa using computerized instruments.
Abstract
We compared the results of sperm analysis using a conventional CASA system (Cell Motion Analyzer, SM-CMA), with those using a novel software (QualiSperm) and those of visual assessment performed by two operators. Ejaculates were collected weekly from four Swedish Landrace boars for 4 weeks. Each eja...
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PMID: 18358522
PDF is available here.
PDF is available here.
Abstract
Manual and automated CSF assays had moderate to excellent correlation for WBC and RBC concentrations, but results were more variable for differential cell percentages. The ADVIA assay may be more useful for assessment of canine CSF with adjustment of cell differentiation algorithms....
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PMID: 18533927
PDF is available here.
PDF is available here.
Abstract
The APOLOWAKO is an entirely automatic benchtop biochemistry analyzer that uses stabilized liquid reagents. It was tested for canine blood and plasma glucose, creatinine, urea, total proteins, alanine aminotransferase, and alkaline phosphatase. The APOLOWAKO gave very similar results for whole blood...
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PMID: 17826812
PDF is available here.
PDF is available here.
Abstract
MEKOC microscopy complexes have a group of specialized automatic functions for medical analyses of biomaterials integrated with general virtual microscopy accessories. Such functions provide a way of making specialized reference virtual slides (RVS). The latter contain the results of virtual analysi...
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PMID: 18720736
PDF is available here.
PDF is available here.
Clinical Chemistry (54)6 2008
Abstract
This simple and scalable homogeneous assay may serve as a convenient, rapid, and accurate screen to detect the presence of pathologic expanded HD alleles in symptomatic patients....
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PMID: 18403567
PDF is available here.
PDF is available here.
Clinical Chemistry (54)6 2008
Abstract
By combining a direct immunoassay with a competitive system, analytes present in widely varying concentrations can be quantified within a single multiplex assay. Introducing a second readout channel for analyte quantification is an effective tool for spot-to-spot normalization and helps to lower int...
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PMID: 18436718
PDF is available here.
PDF is available here.
Clinical Chemistry (54)6 2008
Abstract
Clinical Chemistry (54)5 2008
Abstract
Clinical Chemistry (54)5 2008
Abstract
We describe a sensitive and robust mAb-based TR-IFMA for proGRP. The assay is fully automated and displays high quality performance....
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PMID: 18443179
PDF is available here.
PDF is available here.
Clinica Chimica Acta (391)1-2 2008
Abstract
The Architect BNP assay shows good performance characteristics with total imprecision < or =5.3%. It agrees well with the Advia Centaur, AxSYM, and UniCel DxI BNP assays....
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PMID: 18294456
PDF is available here.
PDF is available here.
International Journal of Epidemiology (37 Suppl 1) 2008
Abstract
Modern manufacturing principles are appropriate for use in the development of a high throughput biological sample processing facility....
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PMID: 18381393
PDF is available here.
PDF is available here.
International Journal of Epidemiology (37 Suppl 1) 2008
Abstract
A specially designed vision system was capable of accurately measuring the position of the plasma meniscus, plasma/buffy coat interface and the red cells/buffy coat interface within a vacutainer. A rack of 24 vacutainers could be processed in <5 min. CONCLUSION: An automated, high throughput blood f...
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PMID: 18381394
PDF is available here.
PDF is available here.
Clinical Chemistry (54)3 2008
Abstract
Clinical Chemistry (54)3 2008
Abstract
The AxSYM Active B12 assay allows rapid, precise, sensitive, specific, and automated measurement of human holoTC in serum and plasma....
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PMID: 18178666
PDF is available here.
PDF is available here.
Journal of Chromatography B (863)1 2008
Abstract
A method for determination of a gamma-secretase inhibitor, cis-3-[4-[(4-chlorophenyl)sulfonyl]-4-(2,5-difluorophenyl)cyclohexyl]propanoic acid (A), in human plasma and cerebrospinal fluid (CSF) has been developed to support the clinical investigation of compound A for its potential treatment of Alzh...
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PMID: 18249589
PDF is available here.
PDF is available here.
Abstract
We demonstrated that paraprotein interferences with TBIL, DBIL, and HDL-C are relatively common and provided explanations why these interferences occurred. Although it is difficult to predict which specimens cause interferences, spurious results appeared method and concentration dependent....
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PMID: 18251580
PDF is available here.
PDF is available here.
Abstract
The performance of the iQ200 is equal to traditional microscopy, but it strongly improves the reliability of urinalysis by standardisation, quantitative reports and improved workflow. From a clinical point of view, renewed attention and improvement of routine urinalysis aids in the efficient detecti...
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PMID: 19012529
PDF is available here.
PDF is available here.
Cell Transplantation (16)10 2008
Abstract
We have developed two methodologies: multiple automated perifusion to determine islet hormone secretion and high-throughput kinetic imaging to examine islet cellular responses. Both technologies use fully automated devices that allow performing simultaneous experiments on multiple islet preparations...
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PMID: 18351020
PDF is available here.
PDF is available here.
Abstract
We assessed the concordance between the results obtained from 652 freshly collected urine samples for erythrocytes (RBC), leukocytes (WBC), squamous epithelial cells (EC), nitrites/bacteria, and crystals using the methodologies mentioned. A principal components analysis was performed in order to exa...
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PMID: 18623125
PDF is available here.
PDF is available here.
Clinical Chemistry (54)1 2008
Abstract
The within-run and between-run (total) imprecision (CVs) were < or =1.7% (n = 20) and 4.4% (n = 15), respectively. The median within-person variability of results (3 measurements over 3 months in 15 postmenopausal women) was 7.2%, resulting in a least significant change (LSC) value of 20%. Serum P1N...
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PMID: 17998267
PDF is available here.
PDF is available here.
Abstract
Differences between assays have implications for public health messages about hypovitaminosis D. Harmonization of results with DEQAS enabled the use of previously determined cutoffs for hypovitaminosis D....
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PMID: 17726070
PDF is available here.
PDF is available here.
Abstract
We developed a semi-automated method for the measurement of urinary iodine using firstly ammonium persulfate for digestion of urine followed by estimation of iodine content in the Sandell-Kolthoff reaction, in which iodine acts as a catalyst for the reduction of cerium. This method was validated in...
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PMID: 18209890
PDF is available here.
PDF is available here.
Clinical Chemistry (53)12 2007
Abstract
Polyoxyethylene-polyoxybutylene block copolymer (POE-POB) exhibited favorable selectivity toward VLDLR and IDL fractions. POE-POB removed apolipoprotein (apo) E and apo C-III from IDL particles in the presence of cholesterol esterase (CHER), and the particle size distribution of IDLs became smaller...
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PMID: 17901111
PDF is available here.
PDF is available here.
Clinical Chemistry (53)12 2007
Abstract
A high-throughput, automated thawing and mixing workcell was successfully built, validated, and installed on our automated transport and sorting system....
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PMID: 17890435
PDF is available here.
PDF is available here.
Abstract
We describe Peptoonist, a program that can automatically identify the glycans (sugars) present at each N-glycosylation site of a protein. The input to Peptoonist is a series of mass spectra, both MS and MS/MS, obtained from a liquid chromatography (LC) run of proteolytically digested purified glycop...
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PMID: 17727280
PDF is available here.
PDF is available here.
Clinical Chemistry (53)10 2007
Abstract
The combined microchip-DHPLC strategy allows rapid and specific molecular diagnosis of craniosynostosis and is an effective tool for the medical and surgical management of these common congenital anomalies in a newborn or an infant with a developmental defect of the cranial vault....
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PMID: 17693524
PDF is available here.
PDF is available here.
Abstract
Agreement rates between M960 and conventional methods were 95.2% with B460, 96.6% with the PM and 93.4% with the RRM. The lowest agreement rates were obtained for SM with the RRM and for EMB with B460. When comparing M960 with judicial susceptibility profiles, the agreement rate was 97.9%. The agree...
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PMID: 17705976
PDF is available here.
PDF is available here.
Abstract
Our results indicate that the automated IG count can replace the manual morphology count for IG counting in the clinical laboratory. The results also confirm that the flow cytometric IG count is superior to and can replace the manual morphology count as a reference method for IG counting....
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PMID: 17709320
PDF is available here.
PDF is available here.
Abstract
Serum BALP activity paralleled PALP activity within each group when compared with the controls. CONCLUSION: Our data indicate that the semiautomated serum BALP isoenzyme assay may be used as a biomarker of bone-building potential in rat models of osteoporosis. This assay affords many advantages to i...
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PMID: 17806079
PDF is available here.
PDF is available here.
Clinical Chemistry (53)9 2007
Abstract
Clinica Chimica Acta (384)1-2 2007
Abstract
These 2 automated urinalysis systems demonstrated good concordance with each other in urine sediment examination. The automated process could be used as a screening procedure but some manual microscopy was still necessary....
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PMID: 17604012
PDF is available here.
PDF is available here.
Clinical Chemistry (53)9 2007
Abstract
This automated high-throughput XLC-MS/MS method for the measurement of plasma free MNs is precise and linear, with short analysis time and low variable costs. The method is attractive for routine diagnosis of pheochromocytoma because of its high analytical sensitivity, the analytical power of MS/MS,...
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PMID: 17712005
PDF is available here.
PDF is available here.
Clinical Chemistry (53)8 2007
Abstract
This newly constructed immunosensor array provides a simple, automated, simultaneous multianalyte immunoassay with high throughput, short analytical time, and sufficiently low detection limits for clinical application. This method offers the capability of miniaturizing the multianalyte detection dev...
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PMID: 17599954
PDF is available here.
PDF is available here.
Abstract
We compared the results of ESR obtained by an ESR analyzer with those by the Westergren method in a group of 75 patients Linear regression analysis showed a good correlation between the two results (r = 0.818, p < 0.01). The intra class correlation was 0.82. The analyzer method had the advantages of...
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PMID: 17883168
PDF is available here.
PDF is available here.
Abstract
Overall correlation between the automated and manual results was excellent. The automated method revealed better precision than the manual method. The number of specimens with false automated results was very small, and all were flagged for verification by a smear review....
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PMID: 17616994
PDF is available here.
PDF is available here.