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Calorimetry (1):
Recent article
Calorimetry
Abstract
With the help of thermonetry and general calorimetry body temperature and heat production in ambient temperatures 20 degrees C, 28 degrees C, 33 degrees C were recorded. The experiments showed, that at the temperature 20 degrees C the rectal temperature was changing very little. But...
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PMID: 21961306
PDF is available here.
PDF is available here.
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Abstract
National recommendations for the prevention and treatment of obesity emphasize reducing energy intake. Foods purchased in restaurants provide approximately 35% of the daily energy intake in US individuals but the accuracy of the energy contents listed for these foods is unknown....
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PMID: 21771989
PDF is available here.
PDF is available here.
Abstract
The accumulation, uptake mechanism, cytotoxicity, cellular localisation of-and mode of cell death induced by-dinuclear ruthenium(II) complexes ΔΔ/ΛΛ-[{Ru(phen)(2) }(2) {μ-bb(n) }](4+) (Rubb(n) ), where phen is 1,10-phenanthroline, bb(n) is bis[4(4'-methyl-2,2'-bipyridyl)]-1,n-al...
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PMID: 21472992
PDF is available here.
PDF is available here.
Biochimica et Biophysica Acta (1814)5 2011
Abstract
We use spectroscopic and calorimetric techniques to elucidate how anionic, cationic and non-ionic surfactants interact with bovine βLG and modulate its heat-induced aggregation. Alkyl trimethyl ammonium chlorides (xTAC) strongly promote aggregation, while sodium alkyl sulfates (SxS) and alkyl malto...
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PMID: 21440683
PDF is available here.
PDF is available here.
Biochimica et Biophysica Acta (1814)8 2011
Abstract
Heme proteins represent a diverse class of biomolecules responsible for an extremely diverse array of physiological functions including electron transport, monooxygenation, ligand transport and storage, cellular signaling, respiration, etc. An intriguing aspect of these proteins is that such function...
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PMID: 21278003
PDF is available here.
PDF is available here.
Biochimica et Biophysica Acta (1808)4 2011
Abstract
By using Fourier transform infrared (FT-IR) spectroscopy in combination with differential scanning calorimetry (DSC) coupled with pressure perturbation calorimetry (PPC), ultrasound velocimetry, Laurdan fluorescence spectroscopy, fluorescence microscopy and atomic force microscopy (AFM), the temperat...
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PMID: 21262194
PDF is available here.
PDF is available here.
Abstract
The study highlighted the main forces driving the formation of hydroxypropyl-cyclodextrins (HP-CDs) + poly(propylene) glycol 725 g mol(-1) inclusion complexes. The temperature parameter was chosen as the variable to modulate the hydrophobicity of the polymer, and consequently ITC experiments as funct...
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PMID: 21666930
PDF is available here.
PDF is available here.
Abstract
Lithiation of N-(4-chlorophenyl)-pivalamide (NCP) and two additional substituted acetanilides: 4-fluoroacetanilide (4-F) and 4-chloroacetanilide (4-Cl) has been monitored by means of calorimetry, on-line ATR-IR and UV/vis spectroscopy and endoscopy. The combined on-line monitoring re...
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PMID: 21678773
PDF is available here.
PDF is available here.
Biochimica et Biophysica Acta (1808)4 2011
Abstract
These results suggest that the PLM cytoplasmic domain populates NKA-associated and membrane-associated states in dynamic equilibrium and that phosphorylation may alter the position of the equilibrium. Interestingly, peptides representing the cytoplasmic domains of two other FXYD proteins, Mat-8 (FXY...
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PMID: 21130070
PDF is available here.
PDF is available here.
Abstract
Inorganic-organic cobalt phyllosilicate hybrids were synthesized by the sol-gel procedure under mild non-hydrothermal conditions with a silicon precursor, formed through individual reactions between the silane 3-glycidoxypropyltriethoxysilane and the aminoalcohols ethanol- or diethanolamine. These p...
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PMID: 20922219
PDF is available here.
PDF is available here.
Abstract
We have previously shown that a 28-amino acid peptide derived from the BRC4 motif of BRCA2 tumor suppressor inhibits selectively human RAD51 recombinase (HsRad51). With the aim of designing better inhibitors for cancer treatment, we combined an in silico docking approach with in vitro biochemical te...
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PMID: 20684611
PDF is available here.
PDF is available here.
Abstract
We really able to understand about complex formation and can any of this information provide a useful tool to aid drug development? Correlations between thermodynamic data and structural detail have been investigated, allowing insight into ways in which these can be used to understand protein-ligand...
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PMID: 20658972
PDF is available here.
PDF is available here.
Abstract
We describe a series of experiments concerning the interaction of chlorocatechol 1,2-dioxygenase from Pseudomonas putida (Pp1,2-CCD) with cis-cis muconic acid. We used single-injection ITC to show that the reaction product inhibits enzyme kinetics. DSC and EPR measurements probed whether this was ac...
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PMID: 20452370
PDF is available here.
PDF is available here.
Bioresource Technology (101)15 2010
Abstract
Equilibrium adsorption experiments, isothermal titration calorimetry and potentiometric titration techniques were employed to investigate the adsorption of Cu(II) by extracellular polymeric substances (EPS) extracted from Pseudomonas putida X4, minerals (montmorillonite and goethite) and their compo...
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PMID: 20227874
PDF is available here.
PDF is available here.
Abstract
These results show that as oxidative radicals induce high proportions of irreversible damages (polymerisation) centrin 2 is highly sensitive to ionising radiation. This could have important consequences on its biological functions....
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PMID: 20586543
PDF is available here.
PDF is available here.
Hypertension Research (33)8 2010
Abstract
These results suggest that different sources of ALA are effective in preventing hypertension related to omega-3 fatty acid deficiency. However, there were other marked differences between the DEF and, in particular, the SUF-C phenotype including lowered body weight, adiposity, leptin and food intake...
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PMID: 20520615
PDF is available here.
PDF is available here.
Journal of Molecular Biology (400)3 2010
Abstract
We focused our study on TM0486 from Thermotoga maritima, which belongs to one of these COGs of unknown function, namely COG0011. The crystal structure of the protein was determined at 2 A resolution. The structure adopts a beta alpha beta beta alpha beta ferredoxin-like fold and assembles as a homot...
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PMID: 20471400
PDF is available here.
PDF is available here.
Journal of Molecular Biology (400)3 2010
Abstract
We report the crystal structures of TYK2, a first in class structure, and JAK3 in complex with PAN-JAK inhibitors CP-690550 ((3R,4R)-3-[4-methyl-3-[N-methyl-N-(7H-pyrrolo[2,3-d]pyrimidin-4-yl)amino]piperidin-1-yl]-3-oxopropionitrile) and CMP-6 (tetracyclic pyridone 2-t-butyl-9-fluoro-3,6-dihydro-7H-...
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PMID: 20478313
PDF is available here.
PDF is available here.
Journal of Biological Chemistry (285)29 2010
Abstract
The roadblock/LC7 dynein light chain is a ubiquitous component of all dyneins and is essential for many diverse processes including proper axonal transport and dendrite growth. In addition, LC7 functions in non-dynein transcriptional activation of the transforming growth factor-beta complex. Crystal...
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PMID: 20472935
PDF is available here.
PDF is available here.
Abstract
To determine pre- and postoperative predictors of energy expenditure in children with congenital heart disease requiring open heart surgery; and to compare measured resting energy expenditure with current predictive equations.
Prospective resting energy expenditure data were collected, using indirec...
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PMID: 20124946
PDF is available here.
PDF is available here.
Abstract
The toxic effects of artesunate and dihydroartemisinin on the growth metabolism of Tetrahymena thermophila BF5 were studied by microcalorimetry. The results showed that: (1) low concentrations of artesunate (
PDF is available here.
PDF is available here.
Protein Science (19)7 2010
Abstract
The role of specific cleavage of transcription repressor proteins by proteases and how this may be related to the emerging theme of dinucleotides as cellular signaling molecules is poorly characterized. The transcription repressor NmrA of Aspergillus nidulans discriminates between ox...
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PMID: 20506376
PDF is available here.
PDF is available here.
Protein Science (19)7 2010
Abstract
The role of specific cleavage of transcription repressor proteins by proteases and how this may be related to the emerging theme of dinucleotides as cellular signaling molecules is poorly characterized. The transcription repressor NmrA of Aspergillus nidulans discriminates between ox...
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PMID: 20506376
PDF is available here.
PDF is available here.
Protein Science (19)7 2010
Abstract
The role of specific cleavage of transcription repressor proteins by proteases and how this may be related to the emerging theme of dinucleotides as cellular signaling molecules is poorly characterized. The transcription repressor NmrA of Aspergillus nidulans discriminates between ox...
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PMID: 20506376
PDF is available here.
PDF is available here.
Protein Science (19)7 2010
Abstract
The role of specific cleavage of transcription repressor proteins by proteases and how this may be related to the emerging theme of dinucleotides as cellular signaling molecules is poorly characterized. The transcription repressor NmrA of Aspergillus nidulans discriminates between ox...
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PMID: 20506376
PDF is available here.
PDF is available here.
Protein Science (19)7 2010
Abstract
The role of specific cleavage of transcription repressor proteins by proteases and how this may be related to the emerging theme of dinucleotides as cellular signaling molecules is poorly characterized. The transcription repressor NmrA of Aspergillus nidulans discriminates between ox...
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PMID: 20506376
PDF is available here.
PDF is available here.
Protein Science (19)7 2010
Abstract
The role of specific cleavage of transcription repressor proteins by proteases and how this may be related to the emerging theme of dinucleotides as cellular signaling molecules is poorly characterized. The transcription repressor NmrA of Aspergillus nidulans discriminates between ox...
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PMID: 20506376
PDF is available here.
PDF is available here.
Protein Science (19)7 2010
Abstract
The role of specific cleavage of transcription repressor proteins by proteases and how this may be related to the emerging theme of dinucleotides as cellular signaling molecules is poorly characterized. The transcription repressor NmrA of Aspergillus nidulans discriminates between ox...
|
PMID: 20506376
PDF is available here.
PDF is available here.
Protein Science (19)7 2010
Abstract
The role of specific cleavage of transcription repressor proteins by proteases and how this may be related to the emerging theme of dinucleotides as cellular signaling molecules is poorly characterized. The transcription repressor NmrA of Aspergillus nidulans discriminates between ox...
|
PMID: 20506376
PDF is available here.
PDF is available here.
Protein Science (19)7 2010
Abstract
The role of specific cleavage of transcription repressor proteins by proteases and how this may be related to the emerging theme of dinucleotides as cellular signaling molecules is poorly characterized. The transcription repressor NmrA of Aspergillus nidulans discriminates between ox...
|
PMID: 20506376
PDF is available here.
PDF is available here.
Protein Science (19)7 2010
Abstract
The role of specific cleavage of transcription repressor proteins by proteases and how this may be related to the emerging theme of dinucleotides as cellular signaling molecules is poorly characterized. The transcription repressor NmrA of Aspergillus nidulans discriminates between ox...
|
PMID: 20506376
PDF is available here.
PDF is available here.
Protein Science (19)7 2010
Abstract
The role of specific cleavage of transcription repressor proteins by proteases and how this may be related to the emerging theme of dinucleotides as cellular signaling molecules is poorly characterized. The transcription repressor NmrA of Aspergillus nidulans discriminates between ox...
|
PMID: 20506376
PDF is available here.
PDF is available here.
Protein Science (19)7 2010
Abstract
The role of specific cleavage of transcription repressor proteins by proteases and how this may be related to the emerging theme of dinucleotides as cellular signaling molecules is poorly characterized. The transcription repressor NmrA of Aspergillus nidulans discriminates between ox...
|
PMID: 20506376
PDF is available here.
PDF is available here.
Protein Science (19)7 2010
Abstract
The role of specific cleavage of transcription repressor proteins by proteases and how this may be related to the emerging theme of dinucleotides as cellular signaling molecules is poorly characterized. The transcription repressor NmrA of Aspergillus nidulans discriminates between ox...
|
PMID: 20506376
PDF is available here.
PDF is available here.
Protein Science (19)7 2010
Abstract
The role of specific cleavage of transcription repressor proteins by proteases and how this may be related to the emerging theme of dinucleotides as cellular signaling molecules is poorly characterized. The transcription repressor NmrA of Aspergillus nidulans discriminates between ox...
|
PMID: 20506376
PDF is available here.
PDF is available here.
Protein Science (19)7 2010
Abstract
The role of specific cleavage of transcription repressor proteins by proteases and how this may be related to the emerging theme of dinucleotides as cellular signaling molecules is poorly characterized. The transcription repressor NmrA of Aspergillus nidulans discriminates between ox...
|
PMID: 20506376
PDF is available here.
PDF is available here.
Protein Science (19)7 2010
Abstract
The role of specific cleavage of transcription repressor proteins by proteases and how this may be related to the emerging theme of dinucleotides as cellular signaling molecules is poorly characterized. The transcription repressor NmrA of Aspergillus nidulans discriminates between ox...
|
PMID: 20506376
PDF is available here.
PDF is available here.
Protein Science (19)7 2010
Abstract
The role of specific cleavage of transcription repressor proteins by proteases and how this may be related to the emerging theme of dinucleotides as cellular signaling molecules is poorly characterized. The transcription repressor NmrA of Aspergillus nidulans discriminates between ox...
|
PMID: 20506376
PDF is available here.
PDF is available here.
Protein Science (19)7 2010
Abstract
The role of specific cleavage of transcription repressor proteins by proteases and how this may be related to the emerging theme of dinucleotides as cellular signaling molecules is poorly characterized. The transcription repressor NmrA of Aspergillus nidulans discriminates between ox...
|
PMID: 20506376
PDF is available here.
PDF is available here.
Protein Science (19)7 2010
Abstract
The role of specific cleavage of transcription repressor proteins by proteases and how this may be related to the emerging theme of dinucleotides as cellular signaling molecules is poorly characterized. The transcription repressor NmrA of Aspergillus nidulans discriminates between ox...
|
PMID: 20506376
PDF is available here.
PDF is available here.
Protein Science (19)7 2010
Abstract
The role of specific cleavage of transcription repressor proteins by proteases and how this may be related to the emerging theme of dinucleotides as cellular signaling molecules is poorly characterized. The transcription repressor NmrA of Aspergillus nidulans discriminates between ox...
|
PMID: 20506376
PDF is available here.
PDF is available here.
Protein Science (19)7 2010
Abstract
The role of specific cleavage of transcription repressor proteins by proteases and how this may be related to the emerging theme of dinucleotides as cellular signaling molecules is poorly characterized. The transcription repressor NmrA of Aspergillus nidulans discriminates between ox...
|
PMID: 20506376
PDF is available here.
PDF is available here.
Protein Science (19)7 2010
Abstract
The role of specific cleavage of transcription repressor proteins by proteases and how this may be related to the emerging theme of dinucleotides as cellular signaling molecules is poorly characterized. The transcription repressor NmrA of Aspergillus nidulans discriminates between ox...
|
PMID: 20506376
PDF is available here.
PDF is available here.
Protein Science (19)7 2010
Abstract
The role of specific cleavage of transcription repressor proteins by proteases and how this may be related to the emerging theme of dinucleotides as cellular signaling molecules is poorly characterized. The transcription repressor NmrA of Aspergillus nidulans discriminates between ox...
|
PMID: 20506376
PDF is available here.
PDF is available here.
Endocrinology (151)7 2010
Abstract
We show that selective overexpression of androgen receptor in muscle cells (myocytes) of transgenic male rats both increases lean mass percentage and reduces fat mass. Similar changes in body composition are observed in human skeletal actin promoter driving expression of androgen receptor (HSA-AR) t...
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PMID: 20427479
PDF is available here.
PDF is available here.
Biochimica et Biophysica Acta (1804)7 2010
Abstract
The stability of bovine lactoperoxidase to denaturation by guanidinium-HCl, urea, or high temperature was examined by differential scanning calorimetry (DSC) and tryptophan fluorescence. The calorimetric scans were observed to be dependent on the heating scan rate, indicating that lactoperoxidase st...
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PMID: 20298816
PDF is available here.
PDF is available here.
Biochimica et Biophysica Acta (1804)7 2010
Abstract
3-deoxy-D-manno-octulosonate 8-phosphate synthase (KDO8PS) catalyzes the reaction between phosphoenol pyruvate and D-arabinose 5-phosphate to generate KDO8P. This reaction is part of the biosynthetic pathway to 3-deoxy-D-manno-octulosonate, a component of the lipopolysaccharide of the Gram-negative...
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PMID: 20406700
PDF is available here.
PDF is available here.
Abstract
Acetonitrile hydratase (ANHase) of Rhodococcus jostii RHA1 is a cobalt-containing enzyme with no significant sequence identity with characterized nitrile hydratases. The ANHase structural genes anhA and anhB are separated by anhE, predicted to encode an 11.1-kDa polypeptide. An anhE deletion mutant d...
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PMID: 20558748
PDF is available here.
PDF is available here.
Abstract
We use isothermal titration calorimetry and stopped-flow fluorometry to determine and analyze the thermodynamics of the human kinesin-5 (Eg5/KSP) ATPase cycle. In the absence of microtubules, the binding interactions of kinesin-5 with both ADP product and ATP substrate involve significant enthalpic...
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PMID: 20558732
PDF is available here.
PDF is available here.
Abstract
We report the crystal structure of the cytosolic domain of PEX3 in complex with a PEX19-derived peptide. PEX3 adopts a novel fold that is best described as a large helical bundle. A hydrophobic groove at the membrane-distal end of PEX3 engages the PEX19 peptide with nanomolar affinity. Mutagenesis e...
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PMID: 20554521
PDF is available here.
PDF is available here.
Journal of Hazardous Materials (178)1-3 2010
Abstract
Chlorine, as a key element causing high temperature corrosion and low efficiency in waste-to-energy plants, and its thermal behavior has widely drawn attention. In this study, the chlorine content in eight fractions of municipal solid waste (MSW) was quantified and characterized using five analytica...
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PMID: 20171781
PDF is available here.
PDF is available here.
Abstract
We show that peptide nucleic acids, as short as six nucleobases, bind very strongly (K(a) > 10(7)) and sequence selectively to a homopurine tract of double helical RNA at pH 5.5. The isothermal titration calorimetry and circular dichroism experiments suggest that the binding mode may be a sequence s...
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PMID: 20527745
PDF is available here.
PDF is available here.
Biological Trace Element Research (135)1-3 2010
Abstract
In this paper, the biological activation of heteropoly complex of molybdotungstosilicate containing lanthanum K(10)H(3)La(SiMo(6)W(5)O(39))(2)x26H(2)O (LaW(5)) was investigated by spectroscopic approach and microcalorimetry under the human physiological conditions. Fluorescence spectroscopy in combi...
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PMID: 19680606
PDF is available here.
PDF is available here.
Biophysical Chemistry (149)1-2 2010
Abstract
We explore the thermodynamic properties of three different fibrils of the peptide hormone glucagon, formed under different salt conditions (glycine, sulfate and NaCl, respectively), and differing considerably in compactness. The three fibrils display a large variation in the specific heat capacity D...
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PMID: 20435401
PDF is available here.
PDF is available here.
Analytica Chimica Acta (668)1 2010
Abstract
A highly selective, interference free biosensor for the measurement of fructose in real syrup samples was developed. The assay is based on the phosphorylation of D(-)fructose to fructose-6-phosphate by hexokinase and subsequent conversion of fructose-6-phosphate to fructose-1,6-biphosphate by fructo...
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PMID: 20457296
PDF is available here.
PDF is available here.
Abstract
We present the results of a thermodynamic investigation of the binding interaction(s) between the pyrenyl-modified surfactants and DNA. The thermodynamics of the interactions have been examined using isothermal titration calorimetry, light scattering, zeta potential, and circular dichroism measureme...
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PMID: 20428564
PDF is available here.
PDF is available here.
Abstract
We report the crystal structures of the wild type and two Parkinson disease-associated variants of the enzyme, S18Y and I93M, bound to a ubiquitin-based suicide substrate, ubiquitin vinyl methyl ester. These structures reveal that ubiquitin vinyl methyl ester binds primarily at two sites on the enzy...
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PMID: 20439756
PDF is available here.
PDF is available here.
Abstract
Lanthanide salts of phytanic acid, an isoprenoid-type amphiphile, have been synthesized and characterized. Elemental analysis and FTIR spectroscopy were used to confirm the formed product and showed that three phytanate anions are complexed with one lanthanide cation. The physicochemical properties...
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PMID: 20039652
PDF is available here.
PDF is available here.
Journal of Bacteriology (192)10 2010
Abstract
We show here that neither NikA nor the membrane-bound NikB and NikC proteins of the Ni(2+) transport system are required for repellent taxis in response to Ni(2+)....
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PMID: 20233931
PDF is available here.
PDF is available here.
Bioconjugate Chemistry (21)4 2010
Abstract
We have used kinetic measurements of luminescence and measurements of binding by isothermal calorimetry to determine essential rate and equilibrium constants for a system that permanently captures modified DOTA chelates for positron imaging. We used that information along with previous results to qu...
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PMID: 20361788
PDF is available here.
PDF is available here.