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Electrophoresis, Disc (0):
Recent article
Electrophoresis, Disc
Abstract
The polyacrylamide gel electrophoresis technique was used for comparison of common protein of body wall tissues in five species of Lumbricidae (Eisenia nordenskioldi, E.fetida, Lumbricus rubellus, Aporrectodea caliginosa, A. longa). The statistic processing of indexes of electrophoretic similarity r...
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PMID: 20387388
PDF is available here.
PDF is available here.
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Proteomics (10)2 2010
Abstract
We investigated proteins differentially expressed between freshly isolated TECs and sinusoid endothelial cells from well-formed rat HCC using 2-D DIGE coupled with MALDI-TOF/TOF MS. Thirty-eight unique proteins were identified to be differentially expressed more than twofold between the two endothel...
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PMID: 19899081
PDF is available here.
PDF is available here.
Genetika (Moskva) (44)9 2008
Abstract
Identical specimens were separated by electrophoresis in two gels to detect and fix peroxidase isozymes. Both gels were stained by Coomassie brilliant blue for detecting proteins. One gel was previously incubated for detecting peroxidase activity. The differences in electrophoretic patterns between...
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PMID: 18846828
PDF is available here.
PDF is available here.
Abstract
It was shown that the detection of proteins in polyacrylamide gel by silver is inhibited by contact with air of the ammonia complex with silver ions used at the first stage of detection. It was proved by experiments on the reduction of silver by ethanolamine from a complex with ethanolamine and by f...
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PMID: 19088749
PDF is available here.
PDF is available here.
Electrophoresis (28)10 2007
Abstract
We present here a multiphasic buffer system designed for this purpose. It avoids the continuous stacking of SDS as it occurs in the 'classical' SDS-PAGE. The system allows complete stacking and destacking of proteins in the 3.5-250 kDa range at acrylamide concentrations as low as 4.5% T (total acryl...
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PMID: 17427254
PDF is available here.
PDF is available here.
Journal of Cardiovascular Pharmacology (47)Supplement 2 2006
Abstract
In the absence of depolarizing agents, 45Ca uptake by synaptosomes of the brain tissue of spontaneously hypertensive rats (SHR) was 40% greater than in normotensive controls. This difference disappeared after membrane treatment with depolarizing agents or with the addition of the Ca channel blocker...
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PMID: 6204143
PDF is available here.
PDF is available here.
Abstract
Nous avons compareé chez cinq souches de souris adultes Quaking (Qk), C57BL/6, CBA, DBA/2 et CS7BR la distribution des protéines cérébrales totales (solubles en milieu salin ou détergent, acides à pH 8,9 ou basiques à pH 4,3) de fractions subcellulaires obtenues par séparation électrophorét...
PDF is available here.
PDF is available here.
Journal of Chromatography A (1079)1-2 2005
Abstract
The present review encompasses ca. 65 years of history of developments in electrokinetic separations, taking as a starting point the year 1937, i.e. the official launching of Tiselius' moving boundary electrophoresis (MBE). The 1950s have been particularly rich in introducing novel methodologies in...
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PMID: 16038288
PDF is available here.
PDF is available here.
Acta Pharmaceutica (55)2 2005
Abstract
Standard electrophoresis methods were used in the qualitative and quantitative protein analyses of cerebrospinal fluid (CSF). Disc electrophoresis was carried out for detection of oligoclonal IgG bands in cerebrospinal fluid on polyacrylamide gel. Pairs of CSF and serum were taken from 30 patients,...
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PMID: 16179135
PDF is available here.
PDF is available here.
Genetika (Moskva) (41)2 2005
Abstract
We studied blood serum albumins in European (Acipencer ruthensis L.) and Siberian (A. ruthensis marsiglii Brandt) sterlet using disk electrophoresis in polyacrylamide gel. The albumins were shown to be controlled by three codominant alleles of a single locus (ALB*a, b, c). In European sterlet, all t...
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PMID: 15810614
PDF is available here.
PDF is available here.
Abstract
Mechanisms of riboflavin oversynthesis in a high flavinogenic mold, Eremothecium ashbyii, were examined in relation to growth, riboflavin formation and related synthases, and medium pH with increasing culture periods. Growth reached maximum at 1 d and then decreased, riboflavin formation proceeded r...
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PMID: 15386926
PDF is available here.
PDF is available here.
Abstract
Abstract
Analytical Biochemistry (313)1 2003
Abstract
We have found that the dialysis membranes employed provide suitable flow rates for the elution buffer, similar to those of an earlier 3.6-cm-diameter device, resulting in the prevention of excess eluate dilution. The efficiency of this device was demonstrated by the fractionation of a bovine serum a...
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PMID: 12576059
PDF is available here.
PDF is available here.
Brazilian Dental Journal (14)1 2003
Abstract
We also studied the LDH activity and isoenzymes of the pulp of incisor teeth. The stage of development of the rat first molar at the age of 15 days and that of the second molar at the age of 18 days was classified as the beginning of root formation. At the age of 15 days, the electrophoretic profile...
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PMID: 12656457
PDF is available here.
PDF is available here.
Ukrainskij biokhimicheskij zhurnal/Ukrayins'kij biokhimichnij zhurnal/Ukrainian biochemical journal (75)3 2003
Abstract
Abstract
The present study deals with a total of 28 cases of Wilson's disease, 50 normal individuals alongwith siblings and parents of eight cases. Male predominance (18 out of 28 cases), a median age of 11 years and universal presence of Kayser-Fleischer (K. F.) ring marked the cardinal features. Furthermor...
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PMID: 12024917
PDF is available here.
PDF is available here.
Nippon rinsho. Japanese journal of clinical medicine (59 Suppl 2) 2001
Abstract
Tsitologiia (43)6 2001
Abstract
We compared the number, activity, and thermostability of separate electromorphs of Triton-soluble acid phosphatase (AcP) revealed by disc-electrophoresis in polyacrylamide gel using 2-naphthyl phosphate (pH 4.0) as a substrate. No differences in the number of AcP electromorphs and their mobility wer...
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PMID: 11534183
PDF is available here.
PDF is available here.
The venomous hair structure, venom and life cycle of Lagoa crispata, a puss caterpillar of Oklahoma.
Abstract
The presence of a unique population of Lagoa crispata, puss caterpillar, in western Oklahoma is reported. A detailed microscopic examination shows the structure of the L. crispata spines resemble the type 4 spines described by [Kawamoto, F., Kumada, N., 1984. Biology and venoms of lepidoptera. In: T...
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PMID: 10736472
PDF is available here.
PDF is available here.
Abstract
A D-galactose binding lectin (SLL-2) was isolated from Sinularia lochmodes, an octocoral, by a combination of affinity chromatography on acid-treated agarose and FPLC on Superdex 200. SLL-2 agglutinated rabbit and horse erythrocytes while SLL-1, a minor component, reacted only with rabbit erythrocyt...
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PMID: 10817910
PDF is available here.
PDF is available here.
Abstract
Scheme of apricot AAT control by Aat-1, Aat-2, Aat-3 loci on the base of formal genetic analysis was created. Determination by three active (a, b, c) and null (n) alleles Aat-1 of interspecies polymorphism of fast migrating components was revealed. Alleles frequencies and heterozygosity of Aat-1 loc...
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PMID: 10857203
PDF is available here.
PDF is available here.
Tsitologiia (42)12 2000
Abstract
Glucose-6-phosphate dehydrogenase (G6PD), acid phosphatase and esterases in free-living amoebae of 7 Amoeba species were investigated with the use of disc-electrophoresis in polyacrylamide gel. The evidence provided is suggestive that the electrophoretic isoenzyme patterns of acid phosphatase and es...
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PMID: 11213727
PDF is available here.
PDF is available here.
Abstract
Three cellobiases, here called cellobiase A, B, and C, from the culture filtrate of Aspergillus niger A20, were purified by precipitation with ammonium sulphate, gel filtration through Sephadex G-75, and column chromatography of DEAE-cellulose. The purified enzymes were homogeneous on polyacrylamide...
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PMID: 10327588
PDF is available here.
PDF is available here.
American Journal of Physiology -- Legacy Content (275)2 Pt 2 1998
Abstract
We have purified a 28-kDa protein from urine using salt precipitation, preparative isoelectric focusing, and sizing chromatography. Amino acid composition and NH2-terminal amino sequence analysis showed complete homology to calgranulin. Calgranulin was found to be a potent inhibitor of CaOx crystal...
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PMID: 9691016
PDF is available here.
PDF is available here.
Abstract
These results suggest that midband as a shoulder of beta-lipoproteins may be a risk factor for CAD in diabetes mellitus with hyperlipidemia....
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PMID: 9768371
PDF is available here.
PDF is available here.
Abstract
A new analytical procedure for determining the fraction composition (FC) of lipoproteins (LP) is developed on the basis of the physical method small-angle X-ray scattering (SAXS). This method quantitatively determines the levels of the basic fractions of LP and their subfractions in plasma or serum...
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PMID: 9608277
PDF is available here.
PDF is available here.
Abstract
The glycoprotein (15-18 kDa) antigen of Mycobacterium tuberculosis H37Rv was affinity isolated on the immunosorbent with monoclonal antibodies S4C1G4 (specific to M. tuberculosis H37Rv; Avdiyenko V. G., Kondrashov S.Yu, Lyashenko S.M.@Probl. Tuberk. 1996, v. 1, p. 6-8 (in Russian). This antigen and...
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PMID: 9613181
PDF is available here.
PDF is available here.
Abstract
A modified discontinuous electrophoretic method for the separation of standard and urinary glycosaminoglycans has been reported. The merits of the method are the simple and easy to handle apparatus, non-requirement of elaborate cooling system, sensitivity and high reproducibility of the results and...
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PMID: 10773911
PDF is available here.
PDF is available here.
Indian Journal of Cancer (34)4 1997
Abstract
We recommend the triangular approach to diagnosis of paraproteinemia with a special emphasis on immunoelectrophoresis for typing multiple myeloma and allied disorders along with disc electrophoresis for the demonstration of urinary Bence Jones protein in the routine set up....
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PMID: 9715537
PDF is available here.
PDF is available here.
Abstract
This study presents a different structural feature for carbonic anhydrase in human erythrocytes. Carbonic anhydrase isozymes (CA-I and CA-II) were purified from an erythrocyte pool of 20 healthy subjects. For purification, Sepharose-4B-L-tyrosine-sulfanilamide affinity column was used. Resnets from...
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PMID: 9413560
PDF is available here.
PDF is available here.
Abstract
Our task was to isolate autoantibodies to low-density lipoproteins (LDLP) and characterize them. CNBr-Sepharose and LDLP-based immunoadsorbent were prepared. Autoantibodies were isolated from a pool of human sera from donors by affinity chromatography in a column with immunoadsorbent. Analysis of th...
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PMID: 9377026
PDF is available here.
PDF is available here.
Abstract
Abstract
Abstract
Cyclic mechanical stretching of the rabbit aortic smooth muscle cells induced a serum-independent increase in the h-caldesmon expression. The expression was more obvious in the cells grown on laminin. The data obtained suggests that the cyclic stretching specifically activates a serum- and matrix-in...
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PMID: 9035810
PDF is available here.
PDF is available here.
Applied and theoretical electrophoresis : the official journal of the International Electrophoresis Society (6)1 1996
Abstract
The use and development of discontinuous buffer systems for the separation of proteins and nucleic acids are reviewed, and the advantages over a continuous buffer system are presented. Emphasis is given to the more recent applications in DNA separations where discontinuous systems have aided in the...
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PMID: 9072074
PDF is available here.
PDF is available here.
Abstract
Reversed micelles of Aerosol-OT (AOT) in heptane were used to purify superoxide dismutase (SOD) from bovine red blood cells. Effects of pH, temperature, concentrations of NaCl and protein in the aqueous phase, and the AOT to protein ratio on the extraction of the concomitant proteins from the aqueou...
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PMID: 8984471
PDF is available here.
PDF is available here.
Abstract
Acetylcholinesterase (AChE) was purified on columns with iminodiacetate Agarose charged with Cu2+, Zn2+, and Ni2+. The best results (a 14-fold purification and more than 30% activity yield) were obtained with Zn2+ used as a complex-forming metal. The preparation had a specific activity of approximat...
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PMID: 8984476
PDF is available here.
PDF is available here.
Anticancer Research (16)4A 1996
Abstract
We have carried out serum glycoprotein electrophoresis on polyacrylamide disc gel in 53 breast cancer patients, at diagnosis as well as during and after therapy. Follow-up samples were divided into complete responders (CR) (n = 138) and nonresponders (NR) (n = 44). Glycoprotein electrophoresis showe...
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PMID: 8712748
PDF is available here.
PDF is available here.
Abstract
A nephritic condition was developed by infecting Swiss Webster albino mice with the malarial parasite Plasmodium berghei NK 65. These animals were tested for urinary protein and the presence of circulating immune complexes using reagent strips and a polyethylene glycol (PEG) precipitation assay. The...
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PMID: 9583966
PDF is available here.
PDF is available here.
Abstract
I showed hemolysin neutralization activity among them. The protective effect of these MAbs against A. pleuropneumoniae infection were examined by passive immunization. Administration of Apx I MAb to mice extended survival time after challenge with serotype 5. The mice showed partial cross-protection...
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PMID: 8519906
PDF is available here.
PDF is available here.
Abstract
We wanted, therefore, to determine the presence and characteristics of non-typeable H. influenzae in sputum samples from patients with CF. In order to do this, we have assessed sputum samples from 55 consecutive clinically stable patients seen routinely at an adult CF out-patient clinic. Quantitativ...
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PMID: 7589381
PDF is available here.
PDF is available here.
Electrophoresis (16)6 1995
Abstract
A scanning beam laser macroscope has been developed which scans an area of 7.5 x 7.5 cm in 5 s. This new imaging system is examined as a potential tool for scanning electrophoretic gels. A specially-designed telecentric, f* theta laser scan lens is used in the instrument to achieve a linear scan and...
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PMID: 7498139
PDF is available here.
PDF is available here.
Electrophoresis (16)6 1995
Abstract
Horizontal gel electrophoresis has previously suffered from lack of an instrumental design that would allow for application of large sample volumes. A recently introduced commercial apparatus (HPGE-1000, LabIntelligence) remedies that problem in application to gel electrophoresis with intermittent s...
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PMID: 7498142
PDF is available here.
PDF is available here.
Abstract
A study was made of the variations in the electrophoretic profile of myofibrillar proteins in the muscles of blue whiting (Micromesistius poutassou R.), horse mackerel (Trachurus trachurus L.) and mackerel (Scomber scombrus L.). It was shown that all species presented different deterioration pattern...
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PMID: 7785353
PDF is available here.
PDF is available here.
Abstract
We report that soluble CD14 isolated from the urine of nephrotic patients (uCD14) contains a potent cytokine inducing activity. CD14 derived from urine appeared to consist of two major polypeptides of about 54 and 48 kDa. In uCD14 isolated from three different nephrotic patients the cytokine-inducin...
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PMID: 7519994
PDF is available here.
PDF is available here.
Biochemistry (Washington) (33)17 1994
Abstract
Heterodimeric quaternary structures for two enzyme complexes from Lactobacillus acidophilus R-26 exhibiting deoxycytidine kinase/deoxyadenosine kinase (I) and deoxyguanosine kinase/deoxyadenosine kinase(II) activities have been proven by the following steps: (1) separation of each complex into two...
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PMID: 8172906
PDF is available here.
PDF is available here.
Journal of Biological Chemistry (269)16 1994
Abstract
I intermediate analogous to that formed by horseradish peroxidase....
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PMID: 8163487
PDF is available here.
PDF is available here.
Electrophoresis (15)8-9 1994
Separation of chlorophyll-protein complexes by Deriphat polyacrylamide gradient gel electrophoresis.
Abstract
I and photosystem II bands when different plant species or the effects of different treatments are compared using whole thylakoid membranes....
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PMID: 7859709
PDF is available here.
PDF is available here.
Zentralblatt für Gynäkologie (116)6 1994
Abstract
We conclude from these data that glomerular functional lesions pregnancy in patients with preexisting glomerulopathies or pregnancy induced renal dysfunction are accompanied in a high rate by moderate disorders of liver function....
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PMID: 8048289
PDF is available here.
PDF is available here.
Abstract
These results strongly suggest that P-gp functions as an active transporter, with a coupling stoichiometry of one MgATP molecule hydrolysed for one verapamil molecule transported....
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PMID: 7902705
PDF is available here.
PDF is available here.
Journal of Bacteriology (175)21 1993
Abstract
These results and the kinetic parameters suggest that the maleylacetate reductase is sufficient to channel the 2,4-D degradation intermediate 2-chloromaleylacetate into the 3-oxoadipate pathway. In a data base search the NH2-terminal sequence of maleylacetate reductase was found to be most similar t...
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PMID: 8226615
PDF is available here.
PDF is available here.
Abstract
We have studied the relationship between the enzyme activity and rose bengal (RB) binding in hepatic cytosol and plasma indocyanine green (ICG) kinetics in patients with various liver diseases. The enzyme activity was measured in samples of hepatic cytosol obtained from 52 patients. In addition, the...
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PMID: 8251611
PDF is available here.
PDF is available here.
Abstract
A capillary permeability-increasing enzyme-2 was purified from the venom of A. caliginosus by ion-exchange chromatography and gel filtration on Sephadex G-100. By this procedure, 3.1 mg of purified enzyme was obtained from 4 g of the venom. The mol. wt of the purified enzyme was estimated to be appr...
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PMID: 8303715
PDF is available here.
PDF is available here.
Journal of Biological Chemistry (268)26 1993
Abstract
Tyrosine-rich acidic matrix protein (TRAMP) is a recently discovered protein that co-purifies with porcine skin lysyl oxidase and is equivalent to the M(r) 22,000 extracellular matrix protein from bovine skin that co-purifies with dermatan sulfate proteoglycans (Cronshaw, A. D., MacBeath, J. R. E.,...
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PMID: 8103522
PDF is available here.
PDF is available here.
Preparative biochemistry (23)3 1993
Abstract
Two kallikreins were identified in a homogenate of bovine pancreas in terms of their differential elution from an anion-exchange chromatography column. The kallikreins were quantified by their ability to release kinin from a partially purified preparation of bovine kininogen. The second kallikrein,...
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PMID: 8361954
PDF is available here.
PDF is available here.
Abstract
The synthesis of 23-kDa and 34-kDa proteins in mouse peritoneal macrophages was enhanced when they were incubated with oxidatively modified low-density lipoprotein (LDL). This response required the oxidation of the lipid of the LDL, because native LDL had little effect and acetylated LDL enhanced th...
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PMID: 8323542
PDF is available here.
PDF is available here.
Cancer Letters (69)3 1993
Abstract
S1, a heterophile antigen present on human sarcoma cell lines in culture, has been previously defined by this laboratory [1,2]. This antigen is also present in guinea-pig kidney. Purification of the antigen to homogeneity has now been achieved by a combination of ammonium sulfate fractionation, DEAE...
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PMID: 8513443
PDF is available here.
PDF is available here.
Abstract
Over a 20-minute period, subjects expectorated 8 samples of whole saliva (EWS) while chewing gum. Flow rates were calculated, and sucrose was analyzed in these samples as well as in saliva collected on filter paper strips from different tooth surfaces. Salivary film velocity (SFV), based on a 0.1-mm...
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PMID: 8501281
PDF is available here.
PDF is available here.
Zentralblatt fur Hygiene und Umweltmedizin = International journal of hygiene and environmental medicine (193)6 1993
Abstract
Microaerophilic "campylobacter-like organisms" (CLO) were isolated from a drinking water reservoir in Germany during a period of some months. The strains were characterized by biotyping (API Campy), serotyping and SDS-Disc-gelelectrophoresis as Arcobacter butzleri. Now this facultative humanpathogen...
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PMID: 8318136
PDF is available here.
PDF is available here.