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Electrophoresis, Gel, Two-Dimensional (0):
Recent article
Electrophoresis, Gel, Two-Dimensional
Molecular BioSystems (7)10 2011
Abstract
We analyzed by proteomics modifications in plasma proteome of patients with AAA. Therefore, the present study analyzed the soluble plasma proteins using two dimensional electrophoresis (2-DE) and mass spectrometry (MS). We identified 33 protein spots, 31 of which show an up-regulation in AAA patient...
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PMID: 21805001
PDF is available here.
PDF is available here.
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Abstract
We present a mouse model in which a polyclonal sheep anti-mouse podocyte Ab caused subepithelial immune complex formation. Mice developed a nephrotic syndrome with severe edema, proteinuria, hypoalbuminemia, and elevated cholesterol and triglycerides. Development of proteinuria was biphasic: an init...
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PMID: 21844386
PDF is available here.
PDF is available here.
Molecular BioSystems (7)9 2011
Abstract
We resolved cytoplasmic and membrane-associated complexes from hESCs and characterised their composition, stoichiometry, and dynamics by denaturing SDS-PAGE. The reliability of the fractionation was examined by western blot analysis of membrane and cytosolic markers. MALDI TOF/TOF mass spectrometry...
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PMID: 21727978
PDF is available here.
PDF is available here.
Abstract
To study and establish a proteome reference map and regulation network of neonatal rat cardiomyocyte.
Cultured cardiomyocytes of neonatal rats were used. All proteins expressed in the cardiomyocytes were separated and identified by two-dimensional polyacrylamide gel e...
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PMID: 21841810
PDF is available here.
PDF is available here.
Proteomics (11)17 2011
Abstract
Lactobacillus acidophilus NCFM is a probiotic bacterium adapted to survive in the gastrointestinal tract and with potential health benefits to the host. Lactitol is a synthetic sugar alcohol used as a sugar replacement in low calorie foods and selectively stimulating growth of L. aci...
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PMID: 21751373
PDF is available here.
PDF is available here.
Molecular BioSystems (7)9 2011
Abstract
We developed an integrated proteomic platform with the combination of 2-D native/SDS-PAGE-based proteomics with co-immunoprecipitation, far-Western blotting, His-tag affinity purification and functional analysis, and used it to investigate the E. coli cytosolic complexome. A total of 24 distinct het...
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PMID: 21717022
PDF is available here.
PDF is available here.
Journal of Proteomics (74)8 2011
Abstract
Spontaneous isoaspartyl formation from aspartyl dehydration or asparaginyl deamidation is a major source of modifications in protein structures. In cells, these conformational changes could be reverted by the protein L-isoaspartyl methyltransferase (PIMT) repair enzyme that converts...
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PMID: 21586350
PDF is available here.
PDF is available here.
Journal of Proteomics (74)8 2011
Abstract
We have analyzed the adaptive responses of Lupinus albus roots to long-term B deficiency. Large morphological differences were observed between plants grown with or without B, and 265 polypeptides were found to be responsive to B deficiency out of a total of 406 polypeptides detected by two-dimensio...
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PMID: 21406259
PDF is available here.
PDF is available here.
Journal of Proteomics (74)8 2011
Abstract
The workhorse for proteomics in non-model plants is classical two-dimensional electrophoresis, a combination of iso-electric focusing and SDS-PAGE. However, membrane proteins with multiple membrane spanning domains are hardly detected on classical 2-DE gels because of their low abund...
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PMID: 21354347
PDF is available here.
PDF is available here.
Journal of Proteomics (74)8 2011
Abstract
Iron is essential for all living organisms and plays a crucial role in pathogenicity. This study presents the first proteome analysis of plasma membranes isolated from pea roots. Protein profiles of four different samples (+Fe, +Fe/Chitosan, -Fe, and -Fe/Chitosan) were compared by na...
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PMID: 21310270
PDF is available here.
PDF is available here.
Abstract
We tested the proteome equalization technology, ProteoMiner, in conjunction with Multidimensional Protein Identification Technology (MudPIT) to determine how the equalization of protein dynamic range could improve shotgun proteomics methods for the analysis of cellular proteomes. Our results suggest...
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PMID: 21702434
PDF is available here.
PDF is available here.
Abstract
We performed comparative proteomic analyses (2D-DIGE and MALDI-TOF/TOF and iTRAQ/SCX-LC-MS/MS) of cells at three different conditions: exponential, late stationary phase, and biofilms. These results were compared with alterations in the proteome resulting from exposure to a biofilm inhibitory compou...
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PMID: 21612302
PDF is available here.
PDF is available here.
Abstract
Loss of pulp due to caries and pulpitis leads to loss of teeth and reduced quality of life. Thus, there is an unmet need for regeneration of pulp. A promising approach is stem cell therapy. Autologous pulp stem/progenitor (CD105(+)) cells were transplanted into a root canal with stro...
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PMID: 21417716
PDF is available here.
PDF is available here.
Proteomics (11)16 2011
Abstract
We analysed hearts of HCRs and LCRs from generation 22 of selection using DIGE and identified proteins from MS database searches. The running capacity of HCRs was six-fold greater than LCRs. DIGE resolved 957 spots and proteins were unambiguously identified in 369 spots. Protein expression profiling...
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PMID: 21751351
PDF is available here.
PDF is available here.
Proteomics (11)16 2011
Abstract
We have used 2-DE coupled with MS to identify proteins differentially expressed in the livers of juvenile gilthead sea bream (Sparus aurata) grown under fish-farm conditions and fed with a 100 mg/kg MA-enriched diet (MA(100)). After the comparison of the protein profiles from MA(100) fed fish and...
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PMID: 21751345
PDF is available here.
PDF is available here.
Biochimica et Biophysica Acta (1814)7 2011
Abstract
I and growth factor receptor-bound protein 2) are involved in regulation of metabolic pathways, protein biosynthesis and signaling transduction. All exhibited a greater than 1.4-fold change, reproducible in three separate experiments consisting of 36 gels in total. Flow cytometry validated the obser...
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PMID: 21513824
PDF is available here.
PDF is available here.
Biochimica et Biophysica Acta (1810)7 2011
Abstract
Multifunctional l-amino acid oxidases (LAAOs) occur widely in snake venoms.
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PMID: 21539897
PDF is available here.
PDF is available here.
Abstract
Our experiments showed that the ratio of total serum Hp-β to the glycosylated form of Hp-β varied among the patients with different liver diseases. The total Hp-β protein expression level was much higher in HCC than LC, while an incremental proportion of fucosylated Hp-β was also observed in LC...
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PMID: 21606158
PDF is available here.
PDF is available here.
Abstract
(i) a time-dependent method with a predefined cutoff value and (ii) a fixed-time-point method with a calculated cutoff. Thereby, SUB1 was proven to be rather reactive with specific immunoglobulin A (IgA), IgM, and IgG of patients with an acute infection. This finding makes this antigen an attractive...
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PMID: 21543561
PDF is available here.
PDF is available here.
Abstract
We have analyzed ESCC tissues and adjacent normal tissues by two-dimensional electrophoresis (2DE) and matrix-assisted laser desorption/ionization-time-of-flight mass spectrometry (MALDI-TOF MS) analysis. The results showed that a total of 104 protein spots with different expression levels were foun...
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PMID: 21517111
PDF is available here.
PDF is available here.
Abstract
Abstract
We utilized a proteomics strategy to characterize proteome-wide alterations between MP-treated MKN28 lines and their untreated counterparts. A total of 97 spots were positively identified as differentially expressed, and of these 62 proteins were up-regulated, whereas 35 proteins were down-regulated...
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PMID: 21574062
PDF is available here.
PDF is available here.
Abstract
We performed a global proteomic profiling to identify the downstream functional proteins of HBx, thereby detecting the mechanisms of action of HBx on virion replication.
HBx in the HepG2.2.15 cell line was knocked down by the transfection of small interfering RNA (siRNA). The replica...
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PMID: 21669574
PDF is available here.
PDF is available here.
Proteomics (11)12 2011
Abstract
We describe the benefits of heat treatment of neutrophils in reducing protein degradation for subsequent proteome analysis. Neutrophils isolated from four healthy volunteers were each divided into three aliquots and subjected to different preparation methods for 2-DE: (i) Heat treatment, (ii) resusp...
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PMID: 21598391
PDF is available here.
PDF is available here.
Proteomics (11)12 2011
Abstract
We identified 46 proteins that were altered at protein expression levels. The protein changes detected by 2-DE/MS were verified by functional protein assays. These identified proteins include some key proteins involved in cellular stress response, metabolism, adhesion, cytoskeletal dynamics, cell gr...
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PMID: 21595037
PDF is available here.
PDF is available here.
Proteomics (11)12 2011
Abstract
We also found differences at post-translational level. The results indicated that late embryogenesis abundant Rab21 was more strongly phosphorylated in the embryos of the sensitive varieties than in the embryos of the tolerant ones. We propose that the differences found in the phosphorylation status...
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PMID: 21595035
PDF is available here.
PDF is available here.
Abstract
We used two dimensional gel electrophoresis (2-DE) over a narrow pH 3-5.6 range since this lies outside the range of highly abundant albumin, transferrin and immunoglobulins. In addition, we used in-solution isoelectric focusing followed by SDS-PAGE to find biomarkers in hepatitis C induced liver ci...
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PMID: 21410221
PDF is available here.
PDF is available here.
Abstract
We have employed two-dimensional electrophoresis to detect and quantitate gene expression effects of candidate chemoprevention compounds in the livers of treated rats. Oltipraz, an inducer of several phase II enzymes, affected a series of at least 26 proteins, most of which were slightly decreased b...
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PMID: 8538186
PDF is available here.
PDF is available here.
Abstract
Childhood pilocytic astrocytoma is the most frequent brain tumor affecting children. Proteomics analysis is currently considered a powerful tool for global evaluation of protein expression and has been widely applied in the field of cancer research. In the present study, a series of...
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PMID: 21466243
PDF is available here.
PDF is available here.
Abstract
We used a comparative approach aiming to identify candidate proteins potentially implicated in these pathways. We examined changes occurring, in the proteome of a Helicoverpa armigera-derived cell line, upon induction by actinomycin D. We identified 13 proteins for which the relative abundance was s...
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PMID: 21452889
PDF is available here.
PDF is available here.
Abstract
We used DIGE to compare protein expression in amastigote and promastigote forms of Leishmania chagasi. Nine hundred amastigote and promastigote spots were visualized. Five amastigote-specific, 25 promastigote-specific, and 10 proteins shared by the two parasite stages were identified. Furthermore, 4...
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PMID: 21355625
PDF is available here.
PDF is available here.
Abstract
We performed comparative proteome analysis of lung tissue from patients with sporadic IPF (n = 14) and human donor lungs (controls, n = 10) using two-dimensional gel electrophoresis and MALDI-TOF-MS. Eighty-nine differentially expressed proteins were identified, from which 51 were up-regulated and 3...
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PMID: 21319792
PDF is available here.
PDF is available here.
Abstract
We established a proteome reference map of the soybean root apex and compared this with the proteome of the differentiated root zone. The root apex samples contained the apical 1 mm of the root, comprising the RAM, quiescent center and root cap. We identified 342 protein spots from 550 excised pro...
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PMID: 21438152
PDF is available here.
PDF is available here.
Xenobiotica (41)5 2011
Abstract
We provide a sample preparation method for quantitative proteomics of cellular fungi. Two different protein extraction methods were compared with focus on reproducibility, minimized proteolytic degradation and protein losses during the sample preparation. In the first preparation the cells were lyse...
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PMID: 18369902
PDF is available here.
PDF is available here.
Xenobiotica (41)5 2011
Abstract
This chapter describes the technology and selected applications in the field of protein analysis for free flow electrophoresis (FFE). FFE is a highly versatile technology for applications in this field as a result of its continuous processing of sample and the possibility of separating almost any ki...
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PMID: 18392590
PDF is available here.
PDF is available here.
Abstract
We found that ITCs induces SW480 cells apoptosis in a dose-dependent manner by using MTT assay, phase contrast microscope and flow cytometry, and the IC50 was calculated to be 77.72 microg/ml, superior to the chemotherapeutical drug 5-flurouracil. Subsequently, 15 altered proteins in ITCs treated SW...
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PMID: 21699074
PDF is available here.
PDF is available here.
Abstract
A comparative study of proteome maps from control and Cd-exposed rat liver was performed using a new technology of two-dimensional liquid chromatography separation method (PF-2D system, Beckman Coulter). Rats were fed for one month 0 or 100 μg Cd g(-1). The between-replicate and bet...
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PMID: 21093054
PDF is available here.
PDF is available here.
Abstract
We show that affinity of self molecules to the glycosaminoglycan dermatan sulfate (DS) is a common property of autoantigens and leads to a specific autoreactive B-1a cell response. Autoimmune ANA/ENA reference sera react preferentially with DS affinity-fractionated cellular proteins. Studying patien...
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PMID: 21514432
PDF is available here.
PDF is available here.
Abstract
To establish stable tetracycline-inducible pancreatic cancer cell lines.
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PMID: 21528072
PDF is available here.
PDF is available here.
Biochimica et Biophysica Acta (1814)4 2011
Abstract
Many aspects of plant metabolism that are involved in plant growth and development are influenced by light-regulated diurnal rhythms as well as endogenous clock-regulated circadian rhythms. To identify the rhythmic proteins in rice, periodically grown (12h light/12h dark cycle) seedlings were harvest...
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PMID: 21300183
PDF is available here.
PDF is available here.
Abstract
Surgical repair of Achilles tendon (AT) rupture should immediately be followed by active tendon mobilization. The optimal time as to when the mobilization should begin is important yet controversial. Early kinesitherapy leads to reduced rehabilitation period. However, an insight into the detailed mec...
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PMID: 21453573
PDF is available here.
PDF is available here.
Abstract
To establish an efficient and high resolution 2-DE (two-dimensional electrophoresis) protocol for root tuber proteome analysis of Rehmannia glutinosa.
Proteins from root tuber of R. glutinosa were extracted by using five different methods and their productivity and pr...
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PMID: 21809567
PDF is available here.
PDF is available here.
Journal of Proteomics (74)4 2011
Abstract
A large series of protein pathway components have been shown to be dysregulated in Down syndrome (DS) brain. No information about pathomechanisms linked to the trisomic state can be obtained from adult DS brain, however, as neurodegeneration occurs from the fourth decade. The aim of...
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PMID: 21262400
PDF is available here.
PDF is available here.
Proteomics (11)7 2011
Abstract
We analyzed proteome dynamics during plasma cell differentiation with combined 2-DE/MS. Thirty-two identified protein spots changed in relative abundance when lipopolysaccharide (LPS)-stimulated primary B cells differentiated into antibody-secreting plasma cells. A correlative analysis of protein an...
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PMID: 21319304
PDF is available here.
PDF is available here.
Proteomics (11)7 2011
Abstract
We describe the first proteomic characterization of the radial nerve cord (RNC) of an echinoderm, the sea star Marthasterias glacialis. The combination of 2-DE with MS (MALDI-TOF/TOF) resulted in the identification of 286 proteins in the RNC. Additionally, 158 proteins were identified in the synapto...
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PMID: 21365759
PDF is available here.
PDF is available here.
Proteomics (11)7 2011
Abstract
We established a 2-DE reference map for INS-1E cells. Using MALDI-TOF/TOF-MS/MS, we identified 546 spots. These included various proteins with an important role in β-cell physiology and with known roles as crucial proteins for diabetes development. We believe that the availability of this reference...
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PMID: 21365744
PDF is available here.
PDF is available here.
Abstract
We propose that cell wall restructuring and superior homeostasis, particularly the management of reactive oxygen species, may render better dehydration-adaptation. To our knowledge, this is the first report on the comprehensive comparison of dehydration-responsive organellar proteome of two genotype...
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PMID: 21348435
PDF is available here.
PDF is available here.
Abstract
We compared the exoproteome of a nasal carrier strain of SA to a genetically similar noncarrier strain. Additionally, the carrier strain biofilm exoproteome was also compared against its planktonic counterpart. Using high throughput proteomics, it was observed that the carrier strain of SA secretes...
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PMID: 21338050
PDF is available here.
PDF is available here.
Proteomics (11)7 2011
Abstract
We analyzed global protein expression patterns in plasma of control and sibutramine-treated rats using proteomic analysis for a better understanding of the two conflicting functions of this drug, appetite regulation, and cardiovascular risk. The control (n=6) and sibutramine-treated groups (n=6) wer...
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PMID: 21337517
PDF is available here.
PDF is available here.
Proteomics (11)7 2011
Abstract
We report the subproteome reference maps of E. coli B REL606 by analyzing cytoplasmic, periplasmic, inner and outer membrane, and extracellular proteomes based on the genome information using experimental and computational approaches. Among the total of 3487 spots, 651 proteins including 410 non-red...
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PMID: 21337514
PDF is available here.
PDF is available here.
Abstract
I dysfunction, but the exact pathways which lead to cell death have not been resolved. 2D-gel electrophoresis profiles of isolated mitochondria from neuroblastoma cells treated with subcytotoxic concentrations of 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP), a well-characterized complex I inh...
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PMID: 21322648
PDF is available here.
PDF is available here.
Proteomics (11)7 2011
Abstract
We analyzed altered protein expressions in various tissues, namely, brain, lung, spleen, and intestine, from 1 Gy-irradiated mice by employing 2-DE analysis. MALDI-TOF MS and peptide mapping identified 25 proteins that showed greater than twofold expressional changes. In order to confirm signific...
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PMID: 21319302
PDF is available here.
PDF is available here.
Abstract
We describe a strategy that circumvents problems related to the biochemical complexity of this sample and the presence of high concentrations of interfering substances. Bile collected from a patient suffering from a biliary stenosis caused by a pancreatic adenocarcinoma was fractionated by a differe...
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PMID: 21314112
PDF is available here.
PDF is available here.
Journal of Proteomics (74)4 2011
Abstract
We employ a comparative iTRAQ-coupled 2D LC-MS/MS system to analyze the protein profile of NPC cell line upon EBV infection. Based on the proteome data and Western blot validation, 12 proteins were found to be significantly up-regulated and associated with signal transduction, cytoskeleton formation...
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PMID: 21296196
PDF is available here.
PDF is available here.
Journal of Proteomics (74)4 2011
Abstract
We have utilized two cellular models of EOC, where transfer of normal chromosome 18 material into the EOC cell lines TOV-112D and TOV-21G induced in vitro and in vivo suppression of their tumourigenic phenotype. A combination of quantitative two-dimensional difference gel electrophoresis (2D-DIGE) a...
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PMID: 21237297
PDF is available here.
PDF is available here.
Journal of Proteomics (74)4 2011
Abstract
We identified sixteen proteins that were markedly altered and involved in mitochondrial function, heat shock protein activity, myocyte cytoskeleton composition and enzyme activity for energy metabolism. We describe, for the first time, a novel pathway (NDPK) that is involved in LV hypertrophy and en...
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PMID: 21237295
PDF is available here.
PDF is available here.
PLoS Genetics (7)4 2011
Abstract
We now identify 14-3-3 proteins as in vivo interaction partners of Exo1, both in yeast and mammalian cells. Yeast 14-3-3-deficient cells fail to induce Mec1-dependent Exo1 hyperphosphorylation and accumulate Exo1-dependent ssDNA gaps at stalled forks, as revealed by electron microscopy. This leads t...
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PMID: 21533173
PDF is available here.
PDF is available here.
Abstract
The assembly of mitochondrial respiratory chain complex IV (cytochrome c oxidase) involves the coordinated action of several assembly chaperones. In Saccharomyces cerevisiae, at least 30 different assembly chaperones have been identified. To date, pathogenic mutations leading to a mitochondrial disor...
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PMID: 21457908
PDF is available here.
PDF is available here.
Abstract
We have identified protein complexes present under different conditions of nitrogen availability by the use of two-dimensional Blue Native / SDS-PAGE and NSI-LC-LTQ-Orbitrap mass spectrometry. We have identified several membrane protein complexes, including components of the ATP synthase, reaction c...
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PMID: 21443180
PDF is available here.
PDF is available here.
Abstract
Increased climatic variability is resulting in an increase of both the frequency and the magnitude of extreme climate events. Therefore, cereals may be exposed to more than one stress event in the growing season, which may ultimately affect crop yield and quality. Here, effects are r...
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PMID: 21433286
PDF is available here.
PDF is available here.