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Welcome to the Pubget Medical Subject Headings (MESH) browser. Click on a topic or subtopic below to explore related papers. In the gold box is the most recent paper about the current MESH term, and below are 20 related papers.
Electrophoresis (11):
- Electrophoresis, Polyacrylamide Gel
- Electrophoresis, Cellulose Acetate
- Isoelectric Focusing
- Immunoelectrophoresis
- Electrophoresis, Capillary
- Electrophoresis, Agar Gel
- Electrophoretic Mobility Shift Assay
- Electrophoresis, Gel, Two-Dimensional
- Electrophoresis, Gel, Pulsed-Field
- Electrophoresis, Starch Gel
- Electrophoresis, Paper
Recent article
Electrophoresis
Abstract
protein glycosylation varies with the physiological and pathological status of the cell. Consequently, analysis of protein-linked glycans has growing importance both in basic glycobiological research and as a potential tool for monitoring the physiological state in humans. Design, setting and partici...
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PMID: 21807702
PDF is available here.
PDF is available here.
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Abstract
Atrial fibrillation (AF) is an important cause of morbidity and mortality after cardiac surgery. The pathogenesis of AF appears to be multifactorial but little is known about the cause-effect relationship of substrate modifications with the onset of the arrhythmia. With the use of modern proteomics,...
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PMID: 21718835
PDF is available here.
PDF is available here.
Abstract
These results clearly demonstrate that MMP-9 is elevated in CSF of dogs with visceral leishmaniasis (VL). Although these results are preliminary, they suggest that MMP-9 might play a role in disruption of blood-brain barrier and/or blood-CSF barrier. While the presence of MMPs in CSF is not a condit...
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PMID: 21332759
PDF is available here.
PDF is available here.
Abstract
We assessed the importance of glycosaminoglycans and sulfur-mediated bonds for the mechanical properties of lens capsules by comparing the stress-strain responses from control and treated pairs of bovine source. No significant change in mechanical properties was observed upon reduction of disulfide b...
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PMID: 21539774
PDF is available here.
PDF is available here.
Biochimica et Biophysica Acta (1808)9 2011
Abstract
We investigated the gradual disassembly of the AcrB trimer and bound DARPin ligands in dependence on laser intensity in solution. At low laser intensity, the release of the detergent molecule micelle from the AcrB/DARPin complex was observed. By increasing laser intensity, dimeric and monomeric AcrB...
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PMID: 21616055
PDF is available here.
PDF is available here.
Electrophoresis (32)9 2011
Abstract
Abstract
The effects of microbial transglutaminase (MTGase, 0.5%, w/w), dietary fiber (konjac flour, Kjc, 1. 0%, w/w) and ultraviolet irradiation, UV (254 nm, 3300 µW/cm(2) and 40 min), on heat-induced gels from gilthead seabream mince (Sparus aurata) were studied. MTGase addition improved texture, force at...
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PMID: 21421672
PDF is available here.
PDF is available here.
Abstract
We report that human mismatch correction reactions in cell-free extracts occur during concomitant nick-dependent nucleosome assembly shaped by the replication histone chaperone CAF-I. Concomitant nucleosome assembly protects the discontinuous mismatch-containing strands from excessive degradation by...
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PMID: 21282622
PDF is available here.
PDF is available here.
Proteomics (11)4 2011
Abstract
The identification, quantitation and global characterisation of all proteins within a given proteome are extremely challenging. This is due to the absolute detection limits of technology as well as the dynamic range in expression of proteins; and the extreme diversity and heterogenei...
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PMID: 21241016
PDF is available here.
PDF is available here.
Abstract
The comet assay is a sensitive electrophoretic method for measuring DNA breaks at the level of single cells, used widely in genotoxicity experiments, biomonitoring, and in fundamental research. Its sensitivity and range of application are increased by the incorporation of an extra step, after lysis...
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PMID: 20972751
PDF is available here.
PDF is available here.
Abstract
Shotgun proteomics originated as a strategy to identify proteins in complex protein mixtures, but it is also possible to obtain information on relative quantitation with some adjustments to the procedure. After protein digestion, the resulting peptide mixture is labelled with isobaric tags. Then, la...
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PMID: 20978983
PDF is available here.
PDF is available here.
Abstract
We designed targeted primers to amplify a short, species-specific DNA fragment for two secretive stream amphibian species in the northwestern region of the United States (Rocky Mountain tailed frogs, Ascaphus montanus, and Idaho giant salamanders, Dicamptodon aterrimus). We tested three DNA extracti...
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PMID: 21818382
PDF is available here.
PDF is available here.
Abstract
Lab On a Chip (10)23 2010
Abstract
We demonstrate for the first time continuous DNA extraction from cell lysate on a microfluidic chip. After mixing bacterial Escherichia coli culture with superparamagnetic bead suspension, lysis and binding buffers, DNA is released from cells and captured by the beads. These DNA carrying beads are c...
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PMID: 20938545
PDF is available here.
PDF is available here.
Abstract
We quantified protein vicinal dithiols within mammalian mitochondria using the vicinal dithiol-specific reagent phenylarsine oxide (PAO). We found 5-15% of thiols exposed on mitochondrial proteins were vicinal dithiols and that these thiols were particularly sensitive to oxidation by hydrogen peroxi...
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PMID: 20836988
PDF is available here.
PDF is available here.
Abstract
Conventional and innovative strategies can be exploited to identify and characterize new allergenic proteins. With the aim of obtaining suggestions for future improvements, this article describes our attempt to understand and describe some of the advantages and pitfalls of the methodologies and proc...
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PMID: 20973644
PDF is available here.
PDF is available here.
Mutation Research (694)1-2 2010
Abstract
We developed a convenient, specific SSB assay using alkaline gel electrophoresis (AGE) coupled with a neutral O-hydroxylamine, O-(tetrahydro-2H-pyran-2-yl)hydroxylamine (OTX). OTX stabilizes abasic sites (AP sites) to prevent their alkaline incision while still allowing for strong alkaline DNA denat...
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PMID: 20851134
PDF is available here.
PDF is available here.
Kidney International (78)10 2010
Abstract
Kidney International (78)10 2010
Abstract
Kidney International (78)10 2010
Abstract
Kidney International (78)10 2010
Abstract
Proteomics (10)18 2010
Abstract
We expand the application of blue native electrophoresis to the separation of mega protein complexes larger than 10 MDa by introducing novel large pore acrylamide gels. We tailored the bis-acrylamide cross-linker amounts relative to the acrylamide monomer to enlarge the pore size of acrylamide gels...
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PMID: 20687061
PDF is available here.
PDF is available here.
Abstract
The bacterial flora of two Japanese traditional fermented fish products, aji-narezushi (salted and long-fermented horse mackerel (Trachurus japonicas) with rice) and iwashi-nukazuke (salted and long-fermented sardine (Sardinops melanostica) with rice bran), was analysed using non-culture-based polym...
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PMID: 20572057
PDF is available here.
PDF is available here.
Journal of Royal Society Interface (7)Suppl_5 2010
Abstract
Electrophoretic deposition (EPD) is attracting increasing attention as an effective technique for the processing of biomaterials, specifically bioactive coatings and biomedical nanostructures. The well-known advantages of EPD for the production of a wide range of microstructures and...
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PMID: 20504802
PDF is available here.
PDF is available here.
Abstract
We investigated whether blood N-glycan changes can be used as a diagnostic biomarker for Alzheimer disease (AD). We used DNA sequencer-assisted, fluorophore-assisted carbohydrate electrophoresis (DSA-FACE) technology to assay N-glycans in sera from 79 autopsy-confirmed dementia patients and 149 heal...
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PMID: 20426627
PDF is available here.
PDF is available here.
Abstract
Boron is reported in this study to stabilize the structure of heme proteins exposed to peroxides. The oxidized heme protein (15 microM) was treated with H(2)O(2) (10mM) in 1M glycine-NaOH buffer (pH 9.2) at 25 degrees C in absence/presence of boron, and characterized by visible absorption spectrosco...
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PMID: 20576510
PDF is available here.
PDF is available here.
Microbiological Research (165)5 2010
Abstract
Although a number of studies have indicated that microbes are key players in nutrient cycling, limitations on how to accurately assess their diversity have constrained further knowledge on the role of microbial diversity in organic matter decomposition in streams. Microbial diversity on leaf litter...
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PMID: 19720514
PDF is available here.
PDF is available here.
Abstract
We have sought homology-dependent structural phenomena based on topological relaxation. Using two-dimensional electrophoresis, we demonstrate a structural transition in supercoiled plasmid molecules containing homologous segments. Atomic force microscopy (AFM) reveals a dumbbell structure in molecul...
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PMID: 20616051
PDF is available here.
PDF is available here.
Abstract
Numerous methods exist for monitoring residual disease in multiple myeloma using peripheral blood, urine, and bone marrow (BM) techniques. This study was conducted in a cohort of 83 patients to compare residual disease detection using serum protein electrophoresis (SPE) and urine protein electrophor...
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PMID: 20551278
PDF is available here.
PDF is available here.
Abstract
We report on one tape that met both these criteria. Three different cases are presented which demonstrate the usefulness of adhesive tape sampling of crime items. Finally, the advantages of the tape collection technique are discussed and guidelines for preferred areas of tape sampling on various cas...
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PMID: 20456579
PDF is available here.
PDF is available here.
Water Research (44)13 2010
Abstract
The formation, breakage and re-growth of flocs were investigated using alum and polyDADMAC to explore the reversibility of floc breakage. There is a significant reversibility of the breakage process, i.e. the broken flocs can re-grow to the size before breakage, when charge neutralization dominates...
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PMID: 20494394
PDF is available here.
PDF is available here.
Lab On a Chip (10)12 2010
Abstract
A microfluidic system was developed for blood plasma separation at high flow rate. This system uses only hydrodynamic forces to separate plasma from whole blood. The microfluidic network features a series of constrictions and bifurcations to enhance the product yield and purity. A maximum purity eff...
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PMID: 20358050
PDF is available here.
PDF is available here.
Abstract
Aggregation kinetics of rotavirus in aqueous solutions and its deposition kinetics on silica surface in the presence of divalent (Ca(2+), Mg(2+)) cations were studied using complementary techniques of time-resolved dynamic light scattering (TR-DLS) and quartz crystal microbalance (QCM). Within a rea...
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PMID: 20481597
PDF is available here.
PDF is available here.
Abstract
A Radial Stagnation Point Flow (RSPF) system coupled with a microscope was used to study deposition of Cryptosporidium parvum oocysts on quartz and Suwannee River Natural Organic Matter (SRNOM)-coated surfaces in solutions with different Ca(2+) or Mg(2+) concentrations. Both untreated and proteinase...
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PMID: 20465262
PDF is available here.
PDF is available here.
Abstract
We measured total urinary GAGs by a method using dimethylmethylene blue (DMB), and followed by isolation and separation of GAGs using high resolution electrophoresis (HRE) technique. Of 760 urine samples analyzed, 40 have abnormal GAGs HRE patterns. Thirty-five of these 40 cases have elevated urinar...
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PMID: 20614724
PDF is available here.
PDF is available here.
Abstract
Our primary goal was to investigate the antioxidant properties of human milk in a combined in vitro digestion/cell culture model that more closely replicates conditions in the gastrointestinal system of the preterm infant.
An in vitro digestion model was developed that incorporates both gastric and...
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PMID: 20386324
PDF is available here.
PDF is available here.
Analgesic, anti-pyretic and DNA cleavage studies of novel pyrimidine derivatives of coumarin moiety.
Abstract
A novel series of 4-[4-(6-phenyl-pyrimidin-4-yl)-phenoxymethyl]-chromen-2-ones [5-7(a-e)] were synthesized from various 4-bromomethyl coumarins 1(a-e). The synthesized compounds were screened for in-vivo analgesic and anti-pyretic activities at a dose of 25 and 100 mg/kg body weight (b.w), respectiv...
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PMID: 20356657
PDF is available here.
PDF is available here.
Lab On a Chip (10)10 2010
Abstract
We demonstrate that the combination of high speed separations on chip with a fast mass spectrometer enables electrophoretic separations with full mass spectra registration within a second. This was accomplished by coupling a microfluidic glass chip with an integrated nanospray emitter to a fast time...
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PMID: 20445873
PDF is available here.
PDF is available here.
Bing du xue bao = Chinese journal of virology / [bian ji, Bing du xue bao bian ji wei yuan hui] (26)3 2010
Abstract
We reported that the repression of a mutated E2 protein of HPV-2 isolated from a patient with huge common wart on the viral early promoter was obviously decreased, and A338V mutation located at the C terminal DNA binding region of E2 protein. In this study, we expressed and purified the recombinant...
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PMID: 20572344
PDF is available here.
PDF is available here.
Nanotechnology (21)16 2010
Abstract
We demonstrate high yield fabrication of field effect transistors (FET) using chemically reduced graphene oxide (RGO) sheets. The RGO sheets suspended in water were assembled between prefabricated gold source and drain electrodes using ac dielectrophoresis. With the application of a backgate voltage...
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PMID: 20348593
PDF is available here.
PDF is available here.
Abstract
We report here a rapid, simple, and simultaneous immunosensing method for two tumor markers, alpha-fetoprotein (AFP) and carcinoembryonic antigen (CEA), by applying the negative-dielectrophoretic (n-DEP) manipulation of microparticles. Microparticles modified with different antibodies rapidly accumu...
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PMID: 20188978
PDF is available here.
PDF is available here.
Journal of Physical Chemistry B (114)12 2010
Abstract
We find that for low fields most DNA molecules follow tortuous paths through the gels, at an angle up to 90 degrees from the field direction, while some molecules find paths along the field lines. In high fields, virtually all of the DNA molecules follow the field lines. In many cases, the molecules...
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PMID: 20205441
PDF is available here.
PDF is available here.
Abstract
We describe an approach to numerical simulation of spiral waves dynamics of large spatial extent, using small computational grids.
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PMID: 20481855
PDF is available here.
PDF is available here.
Palliative Medicine (24)3 2010
Abstract
There is an increasing emphasis on using translational research to examine mechanisms and increase our understanding of disease. Translational research involves the use of basic science techniques to generate new treatments for use in a clinical setting - the so called 'bench to beds...
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PMID: 20123948
PDF is available here.
PDF is available here.
Palliative Medicine (24)3 2010
Abstract
There is an increasing emphasis on using translational research to examine mechanisms and increase our understanding of disease. Translational research involves the use of basic science techniques to generate new treatments for use in a clinical setting - the so called 'bench to beds...
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PMID: 20123948
PDF is available here.
PDF is available here.
Palliative Medicine (24)3 2010
Abstract
There is an increasing emphasis on using translational research to examine mechanisms and increase our understanding of disease. Translational research involves the use of basic science techniques to generate new treatments for use in a clinical setting - the so called 'bench to beds...
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PMID: 20123948
PDF is available here.
PDF is available here.
Palliative Medicine (24)3 2010
Abstract
There is an increasing emphasis on using translational research to examine mechanisms and increase our understanding of disease. Translational research involves the use of basic science techniques to generate new treatments for use in a clinical setting - the so called 'bench to beds...
|
PMID: 20123948
PDF is available here.
PDF is available here.
Palliative Medicine (24)3 2010
Abstract
There is an increasing emphasis on using translational research to examine mechanisms and increase our understanding of disease. Translational research involves the use of basic science techniques to generate new treatments for use in a clinical setting - the so called 'bench to beds...
|
PMID: 20123948
PDF is available here.
PDF is available here.
Palliative Medicine (24)3 2010
Abstract
There is an increasing emphasis on using translational research to examine mechanisms and increase our understanding of disease. Translational research involves the use of basic science techniques to generate new treatments for use in a clinical setting - the so called 'bench to beds...
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PMID: 20123948
PDF is available here.
PDF is available here.
Palliative Medicine (24)3 2010
Abstract
There is an increasing emphasis on using translational research to examine mechanisms and increase our understanding of disease. Translational research involves the use of basic science techniques to generate new treatments for use in a clinical setting - the so called 'bench to beds...
|
PMID: 20123948
PDF is available here.
PDF is available here.
Palliative Medicine (24)3 2010
Abstract
There is an increasing emphasis on using translational research to examine mechanisms and increase our understanding of disease. Translational research involves the use of basic science techniques to generate new treatments for use in a clinical setting - the so called 'bench to beds...
|
PMID: 20123948
PDF is available here.
PDF is available here.
Pediatric Research (67)4 2010
Abstract
We present an observational study of 55 premature infants born weighing less than 1.3 kg within two level III neonatal intensive care units. Endotracheal aspirates (ETA) and nasogastric aspirates (NGA) were studied with denaturing gradient gel electrophoresis (DGGE) profiling to elucidate the total...
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PMID: 20035248
PDF is available here.
PDF is available here.
Abstract
To explore changes in the structure and diversity of activated sludge-derived microbial communities during adaptation to gradual increases in the concentration of wastewater, RAPD-PCR and the combination of PCR amplification of 16S rRNA genes with denaturing gradient gel electrophoresis (DGGE) analy...
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PMID: 20453903
PDF is available here.
PDF is available here.
Abstract
The synthesis of nanoparticles consisting of a magnetite core coated with one or more layers of amino acid (L-arginine, L-lysine, glycine, and L-glutamine) is described in this paper. For all the amino acids it is found that adsorption increases with concentration in solution in the range 0.5-10 mg/...
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PMID: 20096847
PDF is available here.
PDF is available here.
Clinical Nephrology (73)3 2010
Abstract
In the conventional lipid analysis, triglycerides (TG) correlated directly with ACR and inversely with eGFR quartiles (p = 0.01), while LDL, HDL cholesterol did not correlate with change in ACR or eGFR. ACR was directly associated with apoB, total VLDL, medium VLDL, IDL and small LDL particle concen...
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PMID: 20178716
PDF is available here.
PDF is available here.
Georgian medical news ()179 2010
Abstract
67 patients were investigated. From these patients, 35 had been persistent form of Allergic Rhinitis and 32 - intermittent form of pathology. It has been established, that in patients with Allergic Rhinitis the treatment with intranasal electrophoresis of vanadium strenghthens T-cellulal immunity, h...
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PMID: 20234060
PDF is available here.
PDF is available here.
Molecular Biotechnology (44)2 2010
Abstract
I (hypervariable) simple sequence repeats (SSRs). A total of 231 microsatellite repeats were detected from 12,593 EST sequences of turmeric after redundancy elimination. The average density of Class I SSRs accounts to one SSR per 17.96 kb of EST. Mononucleotides were the most abundant class of micro...
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PMID: 19924572
PDF is available here.
PDF is available here.
Zhongguo ji sheng chong xue yu ji sheng chong bing za zhi = Chinese journal of parasitology & parasitic diseases (28)1 2010
Abstract
Among the 10 isolates, 7 MDH bands were found, more with Rm34, Rm47, Rm51, Rm55, and Rm59. All the isolates showed Rm34 and Rm51 bands. 5 bands showed ALP patterns: Rm22, Rm25, Rm28, Rm35, and Rm38. Difference existed with the MDH and ALP patterns among the isolates. CONCLUSION: MDH and ALP patterns...
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PMID: 20411749
PDF is available here.
PDF is available here.
Ecotoxicology (19)2 2010
Abstract
The temporal activity, abundance and diversity of microbial communities were evaluated across a metal-contamination gradient around a Superfund site in Montana. In order to analyze short-term variability, samples were collected from six sites on four occasions over 12 months. Measure...
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PMID: 19771511
PDF is available here.
PDF is available here.
Ecotoxicology (19)2 2010
Abstract
The temporal activity, abundance and diversity of microbial communities were evaluated across a metal-contamination gradient around a Superfund site in Montana. In order to analyze short-term variability, samples were collected from six sites on four occasions over 12 months. Measure...
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PMID: 19771511
PDF is available here.
PDF is available here.