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Welcome to the Pubget Medical Subject Headings (MESH) browser. Click on a topic or subtopic below to explore related papers. In the gold box is the most recent paper about the current MESH term, and below are 20 related papers.
Mass Spectrometry (6):
Recent article
Mass Spectrometry
Journal of Biological Chemistry (286)40 2011
Abstract
We analyzed early activation of murine CD4+ and CD8+ T-lymphocytes at 24 h. We observed a striking and selective up-regulation in the binding of a recombinant soluble form of siglec-E, an inhibitory siglec, which is expressed on several myeloid cell types including antigen-presenting dendritic cells...
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PMID: 21835922
PDF is available here.
PDF is available here.
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Abstract
Treatment of diabetic mice with glibenclamide and crude extract (BE) significantly declined the FBG content. However, amongst the 6 isolated compounds, 3 compounds (C1, C4 and C6) appreciably subsided the exaggerated level of FBG. Simultaneously, glibenclamide, BE, C4 and C6 treatmen...
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PMID: 22013742
PDF is available here.
PDF is available here.
Abstract
We found that 7-dehydrocholesterol (the immediate precursor of cholesterol) is oxidized by P450 7A1 to 7-ketocholesterol (k(cat)/K(m) = 3 × 10(4) m(-1) s(-1)). P450 7A1 converted lathosterol (Δ(5)-dihydro-7-dehydrocholesterol) to a mixture of the 7-keto and 7α,8α-epoxide products (∼1:2 ratio),...
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PMID: 21813643
PDF is available here.
PDF is available here.
Abstract
We show that MerTK is cleaved at proline 485 in murine macrophages. Site-directed deletion of 6 amino acids spanning proline 485 rendered MerTK resistant to proteolysis and suppression of efferocytosis by cleavage-inducing stimuli. LPS is a known inducer of MerTK cleavage, and the intracellular sign...
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PMID: 21828049
PDF is available here.
PDF is available here.
Abstract
We compared plasma protein profiles of four mouse models of lung cancer with profiles of models of pancreatic, ovarian, colon, prostate, and breast cancer and two models of inflammation. A protein signature for Titf1/Nkx2-1, a known lineage-survival oncogene in lung cancer, was found in plasmas of m...
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PMID: 21907921
PDF is available here.
PDF is available here.
Abstract
We demonstrate that endogenous mesenchymal stem cells (MSCs) become activated during treatment with platinum analogs and secrete factors that protect tumor cells against a range of chemotherapeutics. Through a metabolomics approach, we identified two distinct platinum-induced polyunsaturated fatty a...
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PMID: 21907927
PDF is available here.
PDF is available here.
Abstract
Antimicrobial properties of medicinal plants and plant parts such as flowers, roots, fruits, seeds and oils are being used to cure some chronic and acute diseases throughout the world. In the present study, an attempt has been made to isolate and identify the antibacterial compound p...
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PMID: 22013726
PDF is available here.
PDF is available here.
Eukaryotic Cell (10)9 2011
Abstract
We analyzed the structure of the macronuclear TtVPS13A gene, which was found to be composed of 17 exons spanning 12.5 kb and was predicted to encode a protein of 3,475 amino acids (aa). A strain expressing a TtVPS13A-green fluorescent protein (GFP) fusion protein was constructed, and the protein was...
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PMID: 21764909
PDF is available here.
PDF is available here.
Proteomics (11)18 2011
Abstract
We present a simple, time- and cost-efficient approach to tackle the proteome of prokaryotic organisms. To obtain large data sets of complex biological experiments high-throughput and time- and cost-efficient methods still have to be developed and refined. In this study, we combined well-approved te...
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PMID: 21751368
PDF is available here.
PDF is available here.
Abstract
We investigated the abundance and distribution of nucleobases and nucleobase analogs in formic acid extracts of 12 different meteorites by liquid chromatography-mass spectrometry. The Murchison and Lonewolf Nunataks 94102 meteorites contained a diverse suite of nucleobases, which included three unus...
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PMID: 21836052
PDF is available here.
PDF is available here.
Abstract
We have identified these compounds and others in carbonaceous meteorites and/or as low temperature (laboratory) reaction products of pyruvic acid. In meteorites, we observe many as part of three newly reported classes of compounds: keto acids (pyruvic acid and homologs), hydroxy tricarboxylic acids...
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PMID: 21825143
PDF is available here.
PDF is available here.
Journal of Chromatography B (879)24 2011
Abstract
We investigated and compared the profiles of metabolites in nude mice with alcohol-induced liver injury or bearing a HCC xenograft (HCCX). Alcohol-induced liver injury was achieved by daily administration of grain liquor, and HCC xenografts were generated by subcutaneous inoculation of HepG2 cells i...
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PMID: 21763219
PDF is available here.
PDF is available here.
Journal of Proteomics (74)8 2011
Abstract
Spontaneous isoaspartyl formation from aspartyl dehydration or asparaginyl deamidation is a major source of modifications in protein structures. In cells, these conformational changes could be reverted by the protein L-isoaspartyl methyltransferase (PIMT) repair enzyme that converts...
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PMID: 21586350
PDF is available here.
PDF is available here.
Circulation Research (109)4 2011
Abstract
We focus on emerging techniques that may form a coherently integrated pipeline to overcome present limitations to both the discovery and validation processes....
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PMID: 21817166
PDF is available here.
PDF is available here.
Molecular Cell (43)3 2011
Abstract
We performed a time-resolved analysis of the in vivo interactors of cyclins E1, A2, and B1 by quantitative mass spectrometry. This global analysis of context-dependent protein interactions reveals the temporal dynamics of cyclin function in which networks of cyclin-cdk interactions vary according to...
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PMID: 21816347
PDF is available here.
PDF is available here.
Abstract
CCL5 (RANTES) is a proinflammatory chemokine known to activate leukocytes through its receptor, CCR5. Although the monomeric form of CCL5 is sufficient to cause cell migration in vitro, CCL5's propensity for aggregation is essential for migration in vivo, T cell activation and apo...
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PMID: 21827949
PDF is available here.
PDF is available here.
Abstract
We present a Java library to parse the msf files that constitute the output of Proteome Discoverer. The parser is also implemented as a graphical user interface allowing convenient access to the information found in the msf files, and in Rover, a program to analyze and validate quantitative proteomi...
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PMID: 21714566
PDF is available here.
PDF is available here.
Abstract
We previously described a highly sensitive multidimensional chromatographic strategy based on a combination of hydrophilic interaction liquid chromatography and reversed phase chromatography, which allows proteomic analysis with minimal sample losses. Here we apply this strategy to the analysis of a...
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PMID: 21696214
PDF is available here.
PDF is available here.
Abstract
Abstract
One of the central challenges to metabolomics is metabolite identification. Regardless of whether one uses so-called 'targeted' or 'untargeted' metabolomics, eventually all paths lead to the requirement of identifying (and quantifying) certain key metabolites. Indeed, without metabol...
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PMID: 21827274
PDF is available here.
PDF is available here.
Abstract
We have recently reported that when cholesterol is oxidized with an ozone-like oxidant generated by activated mouse neutrophils, secosterol-A is generated which is then converted to secosterol-B by an aldol reaction. To investigate further pathophysiological roles of secosterols, we have developed a...
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PMID: 21871845
PDF is available here.
PDF is available here.
Life Sciences (89)5-6 2011
Abstract
We measured concentrations of LPA and its precursor, lysophosphatidylcholine stored in the urinary bladder and present in the swollen pelvis of the ligated kidney as well as the corresponding blood plasma by liquid chromatography-tandem mass spectrometry.
We found that concentrations...
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PMID: 21693124
PDF is available here.
PDF is available here.
Life Sciences (89)5-6 2011
Abstract
We measured concentrations of LPA and its precursor, lysophosphatidylcholine stored in the urinary bladder and present in the swollen pelvis of the ligated kidney as well as the corresponding blood plasma by liquid chromatography-tandem mass spectrometry.
We found that concentrations...
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PMID: 21693124
PDF is available here.
PDF is available here.
Life Sciences (89)5-6 2011
Abstract
We measured concentrations of LPA and its precursor, lysophosphatidylcholine stored in the urinary bladder and present in the swollen pelvis of the ligated kidney as well as the corresponding blood plasma by liquid chromatography-tandem mass spectrometry.
We found that concentrations...
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PMID: 21693124
PDF is available here.
PDF is available here.
Abstract
Abstract
The Quantitative Assay Database (QuAD), http://proteome.moffitt.org/QUAD/, facilitates widespread implementation of quantitative mass spectrometry in cancer biology and clinical research through sharing of methods and reagents for monitoring protein expression and modification....
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PMID: 21656910
PDF is available here.
PDF is available here.
Abstract
Introduction: Coumadin (R/S-warfarin) is the most widely prescribed oral anticoagulant in the world; nevertheless, its clinical use is complicated by unpredictability in dose requirements to achieve and maintain optimal anticoagulation. Variations in warfarin metabolism among patient...
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PMID: 21480820
PDF is available here.
PDF is available here.
Abstract
Atrial fibrillation (AF) is an important cause of morbidity and mortality after cardiac surgery. The pathogenesis of AF appears to be multifactorial but little is known about the cause-effect relationship of substrate modifications with the onset of the arrhythmia. With the use of modern proteomics,...
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PMID: 21718835
PDF is available here.
PDF is available here.
Abstract
The plasma proteome was analysed as a potential source of markers of radiosensitivity in patients treated with definitive radiotherapy for head and neck cancer.
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PMID: 21351848
PDF is available here.
PDF is available here.
New England Journal of Medicine (364)26 2011
Abstract
We used a combination of genomic approaches, including next-generation sequencing and mass spectrometry-based genotyping, to identify mutations in samples of bone marrow aspirate from 439 patients with myelodysplastic syndromes. We then examined whether the mutation status for each gene was associat...
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PMID: 21714648
PDF is available here.
PDF is available here.
Abstract
(i) a time-dependent method with a predefined cutoff value and (ii) a fixed-time-point method with a calculated cutoff. Thereby, SUB1 was proven to be rather reactive with specific immunoglobulin A (IgA), IgM, and IgG of patients with an acute infection. This finding makes this antigen an attractive...
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PMID: 21543561
PDF is available here.
PDF is available here.
Abstract
We comprehensively describe the spiroacetal formation involved in the biosynthesis of reveromycin A, which inhibits bone resorption and bone metastases of tumor cells by inducing apoptosis in osteoclasts. We performed gene disruption, systematic metabolite analysis, feeding of labeled precursors and...
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PMID: 21642985
PDF is available here.
PDF is available here.
Abstract
A positive correlation between stearoyl-CoA desaturase (SCD)1 expression and metabolic diseases has been reported in rodents and humans. These findings indicate that SCD1 is a promising therapeutic target for the chronic treatment of diabetes and dyslipidemia. The SCD1 enzyme is expressed at high lev...
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PMID: 21642745
PDF is available here.
PDF is available here.
Abstract
We hypothesized that testosterone regulates components of prostate tight junctions which serve as a barrier to inflammation, thus providing a connection between age- and treatment-associated testosterone declines and prostatic pathology. We examined the expression and distribution of tight junction...
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PMID: 21761342
PDF is available here.
PDF is available here.
Abstract
We investigated the effects of naringin, naringenin and quercetin on the pharmacokinetics of doxorubicin in rats. These Citrus flavonoids are known as P-glycoprotein (P-gp) inhibitors and thus suspected to interact with doxorubicin, as shown by in vitro cell studies. Plasma concentrations, tissue dis...
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PMID: 21699081
PDF is available here.
PDF is available here.
Abstract
The biotransformation of digitoxin and some of its derivatives extracted from Digitalis lanata by Streptomyces isolated species was investigated. Cultures of a Streptomyces strain designated EUSA2003B, isolated from an Egyptian soil sample, efficiently induced selective 12beta-hydroxylation of the st...
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PMID: 21699088
PDF is available here.
PDF is available here.
Abstract
FEBS Journal (278)11 2011
Abstract
We report the overexpression of the ARO10 gene product in Escherichia coli and the first detailed in vitro characterization of this enzyme. The enzyme is shown to be an efficient aromatic 2-keto acid decarboxylase, consistent with it playing a major in vivo role in phenylalanine, tryptophan and poss...
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PMID: 21501384
PDF is available here.
PDF is available here.
Proteomics (11)12 2011
Abstract
We describe the benefits of heat treatment of neutrophils in reducing protein degradation for subsequent proteome analysis. Neutrophils isolated from four healthy volunteers were each divided into three aliquots and subjected to different preparation methods for 2-DE: (i) Heat treatment, (ii) resusp...
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PMID: 21598391
PDF is available here.
PDF is available here.
Proteomics (11)12 2011
Abstract
Small-colony variants (SCVs) of Staphylococcus aureus represent a slow-growing subpopulation causing chronic and relapsing infections due to their physiological adaptation on an intracellular lifestyle. In this first proteomic study on physiological changes associated with a natural,...
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PMID: 21595038
PDF is available here.
PDF is available here.
Proteomics (11)12 2011
Abstract
We identified 46 proteins that were altered at protein expression levels. The protein changes detected by 2-DE/MS were verified by functional protein assays. These identified proteins include some key proteins involved in cellular stress response, metabolism, adhesion, cytoskeletal dynamics, cell gr...
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PMID: 21595037
PDF is available here.
PDF is available here.
Proteomics (11)12 2011
Abstract
We also found differences at post-translational level. The results indicated that late embryogenesis abundant Rab21 was more strongly phosphorylated in the embryos of the sensitive varieties than in the embryos of the tolerant ones. We propose that the differences found in the phosphorylation status...
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PMID: 21595035
PDF is available here.
PDF is available here.
Abstract
Experimental and theoretical studies based on mass spectrometry, collision-induced dissociation, and ab initio calculations are performed on the formation and stability of FeO(n)(+) clusters, as well as on their structural, electronic, and magnetic properties. In the mass spectra, clusters with an ev...
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PMID: 21615142
PDF is available here.
PDF is available here.
Biochimica et Biophysica Acta (1814)9 2011
Abstract
We used biophysical and biochemical approaches to directly monitor small molecule binding to Gβγ. Surface plasmon resonance (SPR) analysis indicated that multiple compounds bound directly to Gβγ with affinities in the high nanomolar to low micromolar range but with surprisingly slow on and off r...
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PMID: 21621014
PDF is available here.
PDF is available here.
Cancer Cell (19)5 2011
Abstract
We investigate the mechanisms by which ETS fusions mediate their effects, and find that the product of the predominant ETS gene fusion, TMPRSS2:ERG, interacts in a DNA-independent manner with the enzyme poly (ADP-ribose) polymerase 1 (PARP1) and the catalytic subunit of DNA protein kinase (DNA-PKcs)...
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PMID: 21575865
PDF is available here.
PDF is available here.
Abstract
We describe the design and synthesis of several boronic acid functionalized peptide-based synthetic lectin (SL) libraries, as well as the optimized methodology for obtaining peptide sequences of these SLs. SL libraries were subsequently used to identify SLs with as high as 5-fold selectivity for var...
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PMID: 21405093
PDF is available here.
PDF is available here.
Abstract
Structures of the indole···pyridine dimer and (indole)2···pyridine trimer have been investigated in a supersonic jet using resonant two-photon ionization (R2PI) and IR-UV double resonance spectroscopic techniques combined with quantum chemistry calculations. R2PI spectra of the dimer and the tr...
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PMID: 21634367
PDF is available here.
PDF is available here.
Abstract
We used single-cell "mass cytometry" to examine healthy human bone marrow, measuring 34 parameters simultaneously in single cells (binding of 31 antibodies, viability, DNA content, and relative cell size). The signaling behavior of cell subsets spanning a defined hematopoietic hierarchy was monitore...
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PMID: 21551058
PDF is available here.
PDF is available here.
Abstract
We previously prepared a SREB2-overexpressing transgenic mouse for functional analysis but were unable to confirm SREB2 protein expression level by immunochemical or biochemical methods. In this article, we report mass spectrometric identification and relative quantitative analysis of SREB2 in the f...
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PMID: 21395347
PDF is available here.
PDF is available here.
Abstract
We validate the high degree of sample purity and evaluate several possible sources of bias in the proteomic data. We compare the classes of proteins identified in the current oocyte proteome with protein classes identified in our previously determined oocyte transcriptome. The oocyte proteome appear...
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PMID: 21452892
PDF is available here.
PDF is available here.
Abstract
We used a comparative approach aiming to identify candidate proteins potentially implicated in these pathways. We examined changes occurring, in the proteome of a Helicoverpa armigera-derived cell line, upon induction by actinomycin D. We identified 13 proteins for which the relative abundance was s...
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PMID: 21452889
PDF is available here.
PDF is available here.
Abstract
A new method for enhancing peptide ion identification in proteomics analyses using ion mobility data is presented. Ideally, direct comparisons of experimental drift times (t(D)) with a standard mobility database could be used to rank candidate peptide sequence assignments. Such a dat...
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PMID: 21417239
PDF is available here.
PDF is available here.
Abstract
We were able to showed that PGC1-α expression is dramatically reduced in GRMD compared to healthy muscle. Collectively, these results provide novel insights into the molecular pathology of the clinically relevant animal model of DMD, and indicate that defective energy metabolism is a central hallma...
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PMID: 21410286
PDF is available here.
PDF is available here.
Abstract
We analyzed how Hb exposure could modulate the macrophage phenotype on a proteome level. We identified and relatively quantified 3691 macrophage proteins, representing the largest human macrophage proteome published to date. The Hb polarized macrophage phenotype was characterized by an induced Hb:Hp...
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PMID: 21405025
PDF is available here.
PDF is available here.
Abstract
We present the proteome of metaphase II mouse oocytes to a depth of 3699 proteins, which substantially extends the number of proteins identified until now in mouse oocytes and is comparable by size to the proteome of undifferentiated mouse ES cells. Twenty-eight oocyte proteins, also detected in ES...
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PMID: 21344949
PDF is available here.
PDF is available here.
Abstract
We designed a strategy to assemble two gold nanoclusters at the ferroxidase active sites of ferritin heavy chain. Our studies showed that the resulting nanostructures (Au-Ft) retain not only the intrinsic fluorescence properties of noble metal, but gain enhanced intensity, show a red-shift, and exhi...
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PMID: 21542609
PDF is available here.
PDF is available here.
Abstract
Although the proteome and genome of bacteriophages are well developed, there is little knowledge about metals and their interactions with the phages, even though metals have been observed in stabilizing phage particles. With expanding studies of phage display and its promising applic...
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PMID: 21423961
PDF is available here.
PDF is available here.
Abstract
Investigation of secondary metabolites from the sea fan-derived fungus Curvularia sp. PSU-F22 resulted in isolation of three new metabolites, curvulapyrone (1), curvulalide (2) and curvulalic acid (3) together with six known compounds, modiolides A (4) and B (5), pyrenolide A (6), stagonolide E (7),...
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PMID: 21656354
PDF is available here.
PDF is available here.
Abstract
A new coumarin, 7-[(E)-3',7'-Dimethyl-6'-oxo-2',7'-octadienyl]oxy coumarin (1), together with three known compounds, schinilenol (2), schinindiol (3) and 7-[(E)-7'-hydroxy-3',7'-dimethylocta-2',5'-dienyloxy]-coumarin (4) were isolated from the methylene chloride fraction of Z. schinifolium by normal...
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PMID: 21656356
PDF is available here.
PDF is available here.
Abstract
In the course of searching for cholesteryl ester transfer protein (CETP) inhibitors from natural sources, a new type of CETP inhibitor, [10]-dehydrogingerdione (1), was isolated from the extract of rhizomes of Zingiber officinale Roscoe. By NMR spectroscopic analysis of its (1)HNMR, (13)C-NMR, and (1...
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PMID: 21656357
PDF is available here.
PDF is available here.