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Welcome to the Pubget Medical Subject Headings (MESH) browser. Click on a topic or subtopic below to explore related papers. In the gold box is the most recent paper about the current MESH term, and below are 20 related papers.
Microchip Analytical Procedures (2):
Recent article
Microchip Analytical Procedures
Xenobiotica (41)5 2011
Abstract
The production of recombinant proteins usually involves the exploration of a wide variety of expression and purification methodologies in the pursuit of a strategy tailored to a particular protein. The methods applied are reliant on exploiting individual differences between expression systems or the...
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PMID: 18988024
PDF is available here.
PDF is available here.
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Lab On a Chip (10)23 2010
Abstract
We present a simple, novel one-pot assay for the analysis of antibiotic susceptibility of bacterial biofilms using a microfluidics system where continuous concentration gradients of antibiotics are generated. The results of minimal biofilm eradication concentration (MBEC) clearly confirm that the co...
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PMID: 20938507
PDF is available here.
PDF is available here.
Lab On a Chip (10)23 2010
Abstract
This communication describes a simple method for printing aqueous solutions with millimeter-scale patterns on a variety of substrates using an easily fabricated, paper-based microfluidic device (a paper-based "stamp") as a contact printing device. The device is made from inexpensive materials, and i...
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PMID: 20949218
PDF is available here.
PDF is available here.
Lab On a Chip (10)23 2010
Abstract
A direct molding method for creating a homogeneous, polymer microfluidic channel is presented. By utilizing capillary rise and subsequent absorption of poly(methyl methacrylate) (PMMA) solution into a solvent-permeable poly(dimethyl siloxane) (PDMS) mold, various circular or elliptic polymer microch...
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PMID: 20938498
PDF is available here.
PDF is available here.
Lab On a Chip (10)23 2010
Abstract
We developed a microfluidic device designed to generate stable concentration gradients at low hydrodynamic shear and allowing long term culture of adhering cells. The gradient forms in a culture space between two parallel laminar flow streams of culture medium at two different concentrations of a gi...
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PMID: 20936235
PDF is available here.
PDF is available here.
Lab On a Chip (10)23 2010
Abstract
We demonstrate for the first time continuous DNA extraction from cell lysate on a microfluidic chip. After mixing bacterial Escherichia coli culture with superparamagnetic bead suspension, lysis and binding buffers, DNA is released from cells and captured by the beads. These DNA carrying beads are c...
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PMID: 20938545
PDF is available here.
PDF is available here.
Lab On a Chip (10)23 2010
Abstract
Functional high-density micro-arrays for mass spectrometry enable rapid picolitre-volume aliquoting and ultrasensitive analysis of microscale samples, for example, single cells.
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PMID: 20938499
PDF is available here.
PDF is available here.
Lab On a Chip (10)18 2010
Abstract
Lab On a Chip (10)12 2010
Abstract
A bio-impedance chip has been developed for real-time monitoring of the kinetics of epithelial cell monolayers in vitro. The human bronchial epithelial cell line (16-HBE 14o-) was cultured in Transwells creating a sustainable and interactive model of the airway epithelium. Conducting polymer polypyr...
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PMID: 20379587
PDF is available here.
PDF is available here.
Lab On a Chip (10)10 2010
Abstract
A droplet-based bioreaction microsystem has been developed with automated droplet generation and confinement. On-chip electronic sensing is employed to track the position of the droplets by sensing the oil/aqueous interface in real time. The sensing signal is also used to control the pneumatic suppl...
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PMID: 20445885
PDF is available here.
PDF is available here.
Lab On a Chip (10)10 2010
Abstract
We set out to create an integrated microchemical chip that benefits from microfluidic chip technology in terms of sensitivity and a strong detection methodology provided jointly by padlock probes and rolling circle amplification (RCA). Here, we have integrated padlock probes and RCA into a microchip...
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PMID: 20445878
PDF is available here.
PDF is available here.
Lab On a Chip (10)4 2010
Abstract
We developed serve not only to transport a fluid over a long distance but also for a broad spectrum of biomedical applications such as protein adsorption, cell adhesion and DNA-based biosensors....
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PMID: 20126691
PDF is available here.
PDF is available here.
Optics Express (17)24 2009
Abstract
This paper reports an efficient fabrication of nanostructures on silicon substrates for surface-enhanced Raman scattering (SERS). Silicon wafer substrates in the aqueous solution of silver nitrate were machined by the femtosecond laser direct writing to achieve simultaneously in one-step the generat...
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PMID: 19997399
PDF is available here.
PDF is available here.
Optics Express (17)16 2009
Abstract
We have integrated magneto-optical traps (MOTs) into an atom chip by etching pyramids into a silicon wafer. These have been used to trap atoms on the chip, directly from a room temperature vapor of rubidium. This new atom trapping method provides a simple way to integrate several atom sources on the...
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PMID: 19654820
PDF is available here.
PDF is available here.
Abstract
We reported the synthesis, fabrication, and preliminary evaluation of poly(ethylene glycol) (PEG)-functionalized polymeric microchips that are inherently resistant to protein adsorption without surface modification in capillary electrophoresis (CE). In this study, we investigated the impact of cross...
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PMID: 19572700
PDF is available here.
PDF is available here.
Optics Express (17)15 2009
Abstract
We numerically demonstrated 1 x N multimode interference (MMI) splitters based on dielectric-loaded surface plasmon polariton waveguides (DLSPPW). The dependences of real part of effective index, propagation length and lateral mode profiles of the waveguides on the geometrical parameters are analyze...
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PMID: 19654663
PDF is available here.
PDF is available here.
Optics Express (17)14 2009
Abstract
We have designed, fabricated and characterized poly(dimethylsiloxane) (PDMS) single-mode rib waveguides. PDMS was chosen specifically for the core and cladding. Combined with the soft lithography fabrication techniques, it enables an easy integration of microoptical components for lab-on-a-chip syst...
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PMID: 19582088
PDF is available here.
PDF is available here.
Analytical Biochemistry (393)2 2009
Abstract
We recently found that the biotinylation reaction from thermophilic archaeon Sulfolobus tokodaii has a unique characteristic that the enzyme BPL forms a tight complex with the product, biotinylated BCCP (169 amino acid residues). In the current work, we attempted to apply this characteristic to a no...
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PMID: 19560433
PDF is available here.
PDF is available here.
Abstract
Protein glycosylation involves the addition of monosaccharides in a stepwise process requiring no glycan template. Therefore, identifying the numerous glycoforms, including isomers, can help elucidate the biological function(s) of particular glycans. A method to assess the diversity of the N-linked...
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PMID: 19288519
PDF is available here.
PDF is available here.
Abstract
Analytical Sciences (25)2 2009
Abstract
We have realized a cloned enzyme donor immunoassay (CEDIA) on a microchip in 96 s. CEDIA is a homogeneous immunoassay, based on the bacterial enzyme beta-galactosidase, which was genetically engineered into two inactive fragments: an enzyme donor and an enzyme acceptor. A model analyte was theophyll...
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PMID: 19212044
PDF is available here.
PDF is available here.
Analyst (Cambridge UK) (134)2 2009
Abstract
This paper describes the fabrication and characterization of thin-layer mercury/gold amalgam microelectrodes and their integration with microchip-based flow injection analysis. This microchip platform allows on-chip injection and lysis of erythrocytes followed by selective detection of intracellular...
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PMID: 19173065
PDF is available here.
PDF is available here.
Analytica Chimica Acta (632)2 2009
Abstract
I (cTnI) were analyzed using the EOC. A chemiluminescent signal proportional to the analyte concentration was produced by adding a luminogenic substrate to the tracer enzyme complexed with the analyte on the chip. The luminescent signal was detected in a dark chamber mounted with a cooled charge-cou...
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PMID: 19110101
PDF is available here.
PDF is available here.
Abstract
Biomarkers of exposure and biological effects have frequently been used to monitor populations for their exposure to toxic substances and for the prediction of disease risk, such as cancer. Current interest is focused on improving the use of biomarkers to better understand biological mechanisms for...
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PMID: 19658318
PDF is available here.
PDF is available here.
Tsitologiia (51)10 2009
Abstract
The problem of non-specific binding of quantum dots (QDs) with cells is very important but not fully understood taking into account the possible application of QDs in medical and fundamental studies. The interactions of untargeted CdSe/ZnS QDs with isolated frog muscle fibres, HeLa cells and J774 ce...
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PMID: 19950862
PDF is available here.
PDF is available here.
Optics Express (17)1 2009
Abstract
We evaluate the spontaneous emission rate (Purcell) enhancement for optically-doped metal-dielectric-semiconductor light-emitting structures by considering the behavior of a semiclassical oscillating point dipole placed within the dielectric layer. For a Ag-SiO(2)-Si structure containing emitters at...
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PMID: 19129887
PDF is available here.
PDF is available here.
Optics Express (17)1 2009
Abstract
We propose a novel hybrid optical sensing system for standalone, chip-scale sensing applications. The hybrid optical sensing system detects any spectral shift of the microresonator sensor output by estimating the effective refractive index using maximum likelihood estimation. The performance evaluat...
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PMID: 19129882
PDF is available here.
PDF is available here.
Abstract
Gangliosides (GGs), a large group of sialylated glycosphingolipids, are considered biomarkers of human brain development, aging and certain diseases. Determination of individual GG components in complex mixtures extracted from a human brain represents a fundamental prerequisite for correlating their...
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PMID: 19661562
PDF is available here.
PDF is available here.
Abstract
An integrated microdevice is developed for the analysis of gene expression in single cells. The system captures a single cell, transcribes and amplifies the mRNA, and quantitatively analyzes the products of interest. The key components of the microdevice include integrated nanoliter metering pumps,...
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PMID: 19075237
PDF is available here.
PDF is available here.
Cancer Research (68)23 2008
Abstract
We have developed a p53-focused array, which allows us to investigate, simultaneously, p53 interactions with most of its known target sequences using the chromatin immunoprecipitation (ChIP)-on-chip methodology. Applying this technique to multiple cell types under various growth conditions revealed...
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PMID: 19047144
PDF is available here.
PDF is available here.
Lab On a Chip (8)12 2008
A polymer lab-on-a-chip for reverse transcription (RT)-PCR based point-of-care clinical diagnostics.
Abstract
An innovative polymer lab-on-a-chip (LOC) for reverse transcription (RT)-polymerase chain reaction (PCR) has been designed, fabricated, and characterized for point-of-care testing (POCT) clinical diagnostics. In addition, a portable analyzer that consists of a non-contact infrared (IR) based tempera...
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PMID: 19023475
PDF is available here.
PDF is available here.
Lab On a Chip (8)12 2008
Abstract
This paper presents the development of an easy-to-handle and disposable clinical diagnostic lab-on-a-chip using fully integrated plastic microfluidic components, which has the sampling/identifying capability to make fast and reliable measurements of metabolic parameters from human whole blood. A sma...
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PMID: 19023474
PDF is available here.
PDF is available here.
Journal of Chromatography B (875)2 2008
Abstract
We developed a porous silicon (PSi) affinity chip to detect BNP present at low concentrations in human plasma by matrix assisted laser desorption/ionization-time of flight-mass spectrometry (MALDI-TOF-MS) directly. The PSi surface immobilized with antibodies captured and concentrated BNP through ant...
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PMID: 18948066
PDF is available here.
PDF is available here.
Abstract
We describe here a microchip-based solid-phase extraction method for purification of RNA from biological samples is demonstrated. The method involves the use of silica beads as a solid phase, and the capacity of the device containing silica beads for RNA, RNA in the presence of protein, and DNA was...
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PMID: 18855414
PDF is available here.
PDF is available here.
ChemPhysChem (9)15 2008
Abstract
We survey some of the approaches and techniques to handle minute amounts of fluid volumes in microfluidic systems with special focus on surface acoustic wave driven fluidics, a technique developed in our laboratory. Here, we outline the basics of this technique and demonstrate, for example, how acou...
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PMID: 18932153
PDF is available here.
PDF is available here.
NHS-ester functionalized poly(PEGMA) brushes on silicon surface for covalent protein immobilization.
Abstract
These results suggest that this method which incorporates the properties of polymer brush onto the binding surfaces may be a good strategy suitable for covalent protein immobilization....
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PMID: 18675539
PDF is available here.
PDF is available here.
Abstract
Antimicrobial therapy is one of the main stones of sepsis therapy. A recent study of septic shock patients showed that each hour of delay in antimicrobial administration during the ensuing 6 h after the onset of hypotension was associated with a decrease in survival rates. However, many questions re...
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PMID: 18704013
PDF is available here.
PDF is available here.
Journal of Chromatography A (1206)1 2008
Abstract
Journal of Chromatography A (1206)1 2008
Abstract
We have investigated the simultaneous determination of them using an electrophoretic lab-on-a-chip (microchip capillary electrophoresis, MCE) technique. In this study, in order to establish an MCE assay process without deproteinization, the addition of a zwitterionic additive into the running buffer...
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PMID: 18692851
PDF is available here.
PDF is available here.
Journal of Chromatography A (1206)1 2008
22nd International Symposium on Microscale Bioseparations and Methods for Systems Biology. Foreword.
Abstract
Journal of Chromatography A (1206)1 2008
Abstract
We describe a simple approach to fabricate robust three-dimensional periodic porous nanostructures inside the microchannels. In this approach, the colloidal crystals were first grown inside the microchannel using an evaporation-assisted self-assembly process. Then the void spaces among the colloidal...
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PMID: 18656884
PDF is available here.
PDF is available here.
Biomedical Microdevices (10)5 2008
Abstract
A flexible polymer tube lab-chip integrated with physical and biochemical sensor modules mounted on a flexible spiral structure for measuring physiological (temperature/flow rate) and metabolic data (glucose concentration) in a catheter application was designed, fabricated and characterized in this...
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PMID: 18483863
PDF is available here.
PDF is available here.
Biomedical Microdevices (10)5 2008
Abstract
We report the development of nitrogen doped diamond-like carbon (DLC:N) microelectrodes on a chip to monitor quantal release of catecholamines from cells. Advantages of DLC:N microelectrodes are that they are batch producible at low cost, and are harder and more durable than graphite films. The DLC:...
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PMID: 18493856
PDF is available here.
PDF is available here.
Analytica Chimica Acta (626)1 2008
Abstract
A fluorescence-activated particle counting and sorting system is developed for lab-on-a-chip applications. This system integrates the microfluidic chip, fluorescence excitation and detection, electronic power switch control, and optical visualization. The automatic sorting function is achieved by el...
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PMID: 18761126
PDF is available here.
PDF is available here.
Lab On a Chip (8)9 2008
Abstract
A simple low temperature adhesive 'stamp-and-stick' bonding procedure for lab-on-a-chip glass devices has been tested for capillary electrophoresis applications. This technique involves use of a mask aligner to transfer a UV-curable adhesive selectively onto the top CE substrate which is then aligne...
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PMID: 18818814
PDF is available here.
PDF is available here.
Lab On a Chip (8)9 2008
Abstract
We present a flexible chip-sized scaffold for three dimensional cell cultivation in the form of a microcontainer array. The spatially warped container walls have been provided with micropores, cell adhesion micropatterns and thin film microelectrodes....
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PMID: 18818815
PDF is available here.
PDF is available here.
Abstract
We have developed a chip-based scanometric method for the detection of mercuric ion (Hg (2+)). This method takes advantage of the cooperative binding and catalytic properties of DNA-functionalized gold nanoparticles and the selective binding of a thymine-thymine mismatch for Hg (2+). The limit of de...
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PMID: 18665608
PDF is available here.
PDF is available here.
Abstract
Mean ambient temperatures were 29.0 degrees C (84.2 degrees F), -2.7 degrees C (27.1 degrees F), and 10.4 degrees C (50.8 degrees F) for groups 1 through 3, respectively. Thermal sensor readings varied with ambient temperature and rectal temperature. Rectal temperatures ranged from 36.2 degrees to 4...
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PMID: 18710319
PDF is available here.
PDF is available here.
Nature Medicine (14)8 2008
Abstract
We report the development of a new, miniaturized diagnostic magnetic resonance (DMR) system for multiplexed, quantitative and rapid analysis. By using magnetic particles as a proximity sensor to amplify molecular interactions, the handheld DMR system can perform measurements on unprocessed biologica...
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PMID: 18607350
PDF is available here.
PDF is available here.
Abstract
We here analyzed 10 chronic lymphocytic leukemia (CLL) samples using four different high-resolution platforms: BAC arrays (32K), oligonucleotide arrays (185K, Agilent), and two SNP arrays (250K, Affymetrix and 317K, Illumina). Cross-platform comparison revealed 29 concordantly detected CNAs, includi...
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PMID: 18484635
PDF is available here.
PDF is available here.
Lab On a Chip (8)8 2008
Abstract
We develop here a microfabrication compatible force measurement technique termed as ultrasoft polydimethylsiloxane-based traction force microscopy (UPTFM). This technique is devised for mapping the cellular traction forces imparted on the adhering substrate, so as to depict the physiological state o...
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PMID: 18651073
PDF is available here.
PDF is available here.
Abstract
We demonstrate that the combination of chaotic advection created by surface acoustic waves combined with a temperature increase (Marangoni effect) leads to the same kinetics regime as in standard macroscale conditions. This opens the route for application of the new generation of LOC to efficient or...
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PMID: 18593199
PDF is available here.
PDF is available here.
Abstract
Abstract
To efficiently prevent and treat bovine mastitis and minimize its effect on the dairy industry, a sensitive, rapid, and specific test is required for identifying the mastitis-causing pathogens. In this study, a biochip capable of detecting 7 common species of mastitis-causing pathogens, including Co...
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PMID: 18599851
PDF is available here.
PDF is available here.
Electrophoresis (29)13 2008
Abstract
We compared the methods of photothermal spectroscopy used in different spatial scales, namely thermal-lens spectrometry (TLS) and thermal-lens microscopy (TLM) to enhance the performance parameters in analytical procedures. All of the experimental results were confirmed by theoretical calculation. I...
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PMID: 18546176
PDF is available here.
PDF is available here.
Lab On a Chip (8)7 2008
Abstract
We present a scalable microvalve that integrates well with standard lab on a chip (LOC) implementations, yet which requires essentially no external infrastructure for its operation. This electrically controlled, phase-change microvalve is used to integrate genetic amplification and analysis via capi...
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PMID: 18584081
PDF is available here.
PDF is available here.
Lab On a Chip (8)7 2008
Abstract
We present a novel, on-chip system for the electrokinetic capture of bacterial cells and their identification using the polymerase chain reaction (PCR). The system comprises a glass-silicon platform with a set of micro-channels, -chambers, and -electrodes. A platinum thin film resistor, placed in th...
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PMID: 18584089
PDF is available here.
PDF is available here.
Lab On a Chip (8)7 2008
Abstract
We investigate viscoelastic electroosmotic flows through a rectangular straight microchannel with and without pressure gradient. It is shown that the volumetric flow rates of viscoelastic fluids are significantly different from those of Newtonian fluids under the same external electric field and pre...
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PMID: 18584093
PDF is available here.
PDF is available here.
Lab On a Chip (8)7 2008
Abstract
This paper presents the capability of changing the activation energy of chemical reactions using microchannel laminar flow. Kinetic parameters of the duplex-coil equilibrium of DNA oligomers were studied by measuring the hysteresis between denaturation-renaturation curves using an in-house temperatu...
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PMID: 18584094
PDF is available here.
PDF is available here.