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Bacterial Typing Techniques (3):
Recent article
Bacterial Typing Techniques
Abstract
We examine the role of S. aureus virulence factors for gut colonization. S. aureus isolated from quantitative stool cultures of 49 Swedish infants followed from birth to 12 months of age were assessed for 30 virulence-associated genes, spa type, and agr allele by serial polymerase chain reaction (PC...
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PMID: 21844297
PDF is available here.
PDF is available here.
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Cell Host & Microbe (10)2 2011
Abstract
We used massively parallel cDNA sequencing (RNA-seq) techniques to quantitatively catalog the transcriptome of the cholera pathogen, Vibrio cholerae, derived from two animal models of infection. Transcripts elevated in infected rabbits and mice relative to laboratory media derive from the major know...
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PMID: 21843873
PDF is available here.
PDF is available here.
Abstract
Actinobaculum schaalii, which belongs to the group of Gram-positive rods, is difficult to culture. Using molecular genetics, Actinobaculum schaalii could be identified as a causing microorganism in a case of Fournier's gangrene.
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PMID: 21508151
PDF is available here.
PDF is available here.
Abstract
Capsule-forming Klebsiella pneumoniae K1 caused primary liver abscess in two household members of a family. The causative isolates had identical pulsed-field gel electrophoresis patterns and were determined to be sequence type 23. An additional member of the family was found to carry the same strain...
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PMID: 21490191
PDF is available here.
PDF is available here.
Abstract
We emphasize the importance of recognizing bacteria that live in the larvae of a parasitic fly as the causative agent of severe infections in homeless patients....
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PMID: 21471333
PDF is available here.
PDF is available here.
Abstract
This paper details the phenotypic, genotypic, and antibiotic sensitivity patterns of 88 Vibrio cholerae strains from Zimbabwe. Of the 88 strains, 83 were classified as "altered El Tor" and 5 as "hybrid El Tor" strains. All of the strains were susceptible to tetracycline, doxycycline, ciprofloxacin, a...
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PMID: 21471347
PDF is available here.
PDF is available here.
Abstract
We present here data for the spread of Pseudomonas fluorescens caused by a contaminated drinking water dispenser in a bone marrow transplant unit. Over a 1-month period we observed a sharp increase in the isolation of P. fluorescens from weekly pharyngeal surveillance swabs. Environmental samples we...
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PMID: 21450958
PDF is available here.
PDF is available here.
Abstract
We investigated the prevalence of 6C among pediatric isolates obtained from eight children's hospitals in the United States. S. pneumoniae isolates were identified from a prospective multicenter study (1993 to 2009). Fifty-seven serotype 6C isolates were identified by multiplex PCR and/or Quellung r...
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PMID: 21450963
PDF is available here.
PDF is available here.
Abstract
A total of 91 of 1,480 invasive isolates (6.1%) collected from adults in Barcelona, Spain, in the period of 1994 to 2008 were of serogroup 6 (6B, 47 isolates; 6A, 28; and 6C, 16). Throughout this period, serotype 6B (Spain(6B)-ST90) decreased, and serotype 6A remained stable. An increase in serotype...
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PMID: 21450964
PDF is available here.
PDF is available here.
Abstract
We performed species identification, examined the antibiotic resistance profiles, and determined the mechanisms of resistance and clonal relationships of 123 Acinetobacter isolates obtained from inmates of 20 California correctional facilities (CCFs). We found that 57.7% of the isolates belong to A....
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PMID: 21450955
PDF is available here.
PDF is available here.
Abstract
Abstract A potential factor leading to the spread of antimicrobial resistance (AR) in bacteria is the horizontal transfer of resistance genes between bacteria in animals or their environment. To investigate this, swine fecal samples were collected on-farm and cultured for Escherichia...
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PMID: 21385089
PDF is available here.
PDF is available here.
Abstract
The objectives of this study were to determine drug resistance pattern in new and previously treated tuberculosis (TB) patients, to assess function of TB control program, and to characterize multidrug resistant TB (MDR-TB) by molecular fingerprinting methods. Anti-microbial susceptib...
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PMID: 21338958
PDF is available here.
PDF is available here.
Leprosy Review (82)1 2011
Abstract
Abstract
The paper describes a rapid method for PCR identification of Groups C and G streptococci (Streptococcus dysgalactiae subspecies equisimilis, Streptococcus constellatus, and Streptococcus anginosus) that cause human disease. Species-specific regions of the cpn60 gene encoding heat shock protein GroEL...
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PMID: 21506385
PDF is available here.
PDF is available here.
Sexual Health (8)1 2011
Abstract
New variant Chlamydia trachomatis (nvCT) remains an important public health concern and in 2008 four cases of nvCT were reported in Scotland. The present study set out to determine whether nvCT was present in England and Wales.
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PMID: 21371386
PDF is available here.
PDF is available here.
Abstract
ABSTRACT: Salmonella enterica subsp. enterica serovar Enteritidis (S. Enteritidis) was responsible for a worldwide pandemic during the 1980s and/or 1990s; however, changes in the dominant lineage before and after this event remain unknown. This study determined Salmonella enterica su...
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PMID: 21672260
PDF is available here.
PDF is available here.
Abstract
We investigated the overall composition of the gut microbiota and its host specificity and temporal stability in 20 Koreans using 454-pyrosequencing with barcoded primers targeting the V1 to V3 region of the bacterial 16S rRNA gene. A total of 303,402 high quality reads covered each sample and 8,427...
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PMID: 21829445
PDF is available here.
PDF is available here.
Abstract
I (n = 1), VIII (n = 1) and five unnamed types (n = 7), while 23 isolates had two types, III+V (n = 12), II+V (n = 8), II+IV (n = 2) or IV+V (n = 1). The five unnamed types were assigned UT1 (class A mec, ccrA1/ccrB4), UT2 (class C1 mec, ccrA4/ccrB4), UT3 (class A mec, cc...
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PMID: 21637845
PDF is available here.
PDF is available here.
Abstract
We tested this genotyping system for molecular epidemiological analysis of 369 M. tuberculosis isolates from 3 regions of Brazil, a high TB-burden country. Deligotyping, targeting 43 large sequence polymorphisms (LSPs), and the MIRU-VNTRplus identification database were used to assess phylogenetic p...
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PMID: 21464915
PDF is available here.
PDF is available here.
Abstract
Irish breeder and intensive broiler flocks together with the corresponding poultry farm environment were sampled for the presence of Campylobacter with the aim of identifying potential sources and transmission routes of poultry flock contamination. The genetic diversity of a subset o...
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PMID: 21039133
PDF is available here.
PDF is available here.
Abstract
We used an enrichment method according to the International Organisation of Standardization (ISO) standard in combination with a real-time polymerase chain reaction (PCR) method detecting the ail gene of Y. enterocolitica. After detecting Y. enterocolitica in a sample, a quantitative investigation o...
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PMID: 21034270
PDF is available here.
PDF is available here.
Khirurgiia ()6 2011
Abstract
The experience of surgical treatment of 1532 patients with the complicated forms of diabetic foot syndrome (DFS) was analyzed. The original clinical classification of the complicated DFS was suggested. The classification considered the clinical form (symbol "C"), anatomic localization (symbol "Z") an...
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PMID: 21716224
PDF is available here.
PDF is available here.
Abstract
The goal of this work was to provide comparative genetic characterization of the human and animal Brucella melitensis isolates from Mongolia, Russia and Azerbaijan using current molecular-genetic typing methods.
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PMID: 21786630
PDF is available here.
PDF is available here.
Abstract
The modern phenotypic and genetic methods except for Multi Locus Sequence Typing do not allow the reliable differentiation within Mitis group of α-hemolytic streptococci. During this study the MALDI mass spectra were acquired for 28 clinical isolates initially identified as S. pneumoniae by routine...
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PMID: 21460882
PDF is available here.
PDF is available here.
Abstract
The phenotypic and genotypic characteristics of 6 clinical strains of Vibrio cholerae isolated in Surabaya, Indonesia in 2009 were examined. The DNA fingerprints obtained suggested that these isolates were not from a single clone. Furthermore, all isolates produced cholera toxin and possessed the cla...
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PMID: 21266749
PDF is available here.
PDF is available here.
Abstract
We present a case of Neisseria meningitidis-related sepsis and meningitis in a 60-year-old woman. The N. meningitidis strain was identified as serogroup B and sequence type (ST)-4893 by multilocus sequence typing (MLST). The patient in this case had visited France prior to development of symptoms. N...
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PMID: 21266758
PDF is available here.
PDF is available here.
Abstract
We have developed a cytolethal distending toxin (cdt) gene-based species-specific multiplex PCR assay for the detection and identification of Campylobacter jejuni, C. coli, and C. fetus. The applicability of this assay was evaluated with 325 Campylobacter strains isolated from diarrheal patients in J...
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PMID: 21266751
PDF is available here.
PDF is available here.
Abstract
Species of Acanthamoeba, known to cause keratitis (AK) and granulomatous encephalitis in humans are frequently isolated from a variety of water sources. In this study, 13 Acanthamoeba isolates from swimming pools were classified at the genotype level based on the sequence analysis of the Acanthamoeba...
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PMID: 21195045
PDF is available here.
PDF is available here.
Cytometry Part A (77)12 2010
Discovering the unknown: detection of emerging pathogens using a label-free light-scattering system.
Abstract
Our study uses a Bayesian approach to learning with a nonexhaustive training dataset to allow for the automated detection of unknown bacterial classes.
© 2010 International Society for Advancement of Cytometry....
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PMID: 21108360
PDF is available here.
PDF is available here.
Abstract
We report the presence of the pathogen Anaplasma phagocytophilum in hedgehogs and their ticks in Germany....
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PMID: 20426690
PDF is available here.
PDF is available here.
Molecular characteristics of "Mycobacterium canettii" the smooth Mycobacterium tuberculosis bacilli.
Abstract
We have collected 59 SmTB isolates of which 14 were newly recovered since previous reports, and performed extensive phenotypical and genotypical characterization. We take advantage of these investigations to review the current knowledge of "M. canettii". Their characteristics and the apparent lack o...
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PMID: 20692377
PDF is available here.
PDF is available here.
Epidemiology and Infection (138)12 2010
Abstract
A case-control and a case-crossover study were performed to investigate a Campylobacter jejuni outbreak in Crete in 2009. Most cases originated from rural areas, served by a different water-supply system from that of the adjacent town. Thirty-seven cases and 79 controls were interviewed; cases were...
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PMID: 20836911
PDF is available here.
PDF is available here.
Epidemiology and Infection (138)12 2010
Abstract
We identified other unpublished reports of outbreaks associated with bike races in rainy or muddy conditions; these underscore the importance of educating racers and raising public awareness of the risks of mud ingestion....
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PMID: 20334726
PDF is available here.
PDF is available here.
Epidemiology and Infection (138)12 2010
Abstract
We believe it is essential to include exposure to mist machines in any legionellosis epidemiological survey....
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PMID: 20392306
PDF is available here.
PDF is available here.
Epidemiology and Infection (138)12 2010
Abstract
We collected respiratory tract samples of adult outpatients with symptoms of pneumonia in a German nationwide network and detected M. pneumoniae by real-time PCR in 140 specimens. The strains were typed by sequencing and demonstrated the circulation of subtypes 1 and 2 and variants 2a and 2b. The ov...
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PMID: 20334729
PDF is available here.
PDF is available here.
Epidemiology and Infection (138)12 2010
Abstract
During a 15-month period in Scotland a small but important number of human Campylobacter cases (3·2%) arose from 91 putative household outbreaks. Of the 26 outbreaks with known strain composition, 89% were composed of the same MLST which supports the potential use of MLST in public health epidemiol...
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PMID: 20587120
PDF is available here.
PDF is available here.
Euro surveillance : bulletin europeen sur les maladies transmissibles = European communicable disease bulletin (15)46 2010
Abstract
We tested the prevalence of MRSA among employees who work in the cold meat processing industry and in institutional kitchens. Nasal swabs and samples from the employees' hands as well as the handled meat were tested quantitatively and qualitatively for the presence of MRSA. Typical colonies were con...
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PMID: 21144430
PDF is available here.
PDF is available here.
British Journal of Nutrition (104)9 2010
Abstract
Epidemiological studies have shown an inverse relationship between risk of CVD and intake of whole grain (WG)-rich food. Regular consumption of breakfast cereals can provide not only an increase in dietary WG but also improvements to cardiovascular health. Various mechanisms have been proposed, incl...
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PMID: 20487589
PDF is available here.
PDF is available here.
Abstract
Our results confirm that the upper respiratory tract acts as an important reservoir of P aeruginosa colonization and infection in the mechanically ventilated patients and emphasize the importance of exogenous acquisition of P aeruginosa. A combination of early identification and eradication of airwa...
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PMID: 20627453
PDF is available here.
PDF is available here.
Abstract
We compared DL to pulsed-field gel electrophoresis (PFGE) on a national collection of ESBL-producing Escherichia coli (n=258; of which 226 isolates were typeable with PFGE) and Klebsiella pneumoniae (n=48) isolated in 2007. For E. coli the Wallace coefficients showed that the probability of two isol...
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PMID: 20849889
PDF is available here.
PDF is available here.
Abstract
We developed the high-throughput Universal Probe Salmonella Serotyping (UPSS) technique based on nano liter PCR. In comparison to the classical approach, where O- and H-antisera are applied, the UPSS relies on specific gene content amplification of Salmonella spp. by a universal TaqMan assay for all...
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PMID: 20869995
PDF is available here.
PDF is available here.
Epidemiologie, mikrobiologie, imunologie : casopis Spolecnosti pro epidemiologii a mikrobiologii Ceske lekarske spolecnosti J.E. Purkyne (59)4 2010
Abstract
A brief report is presented of the results of the Project of the Internal Grant Agency of the Ministry of Health of the Czech Republic that received the Medical Research Award 2008 from the Minister of Health of the Czech Republic (http://www.mzcr.cz/Odbornik/Pages/816-udeleni-ceny-ministra-zdravotn...
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PMID: 21114007
PDF is available here.
PDF is available here.
Journal of Dairy Science (93)10 2010
Abstract
The objective of this study was to evaluate the fermentation dynamics of 2 commonly fed corn (co)products in their intact and defatted forms, using the in vitro gas production (IVGP) technique, and to investigate the shifts of the predominant rumen bacterial populations using the 16S rDNA bacterial...
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PMID: 20855008
PDF is available here.
PDF is available here.
Abstract
This review aims to identify and highlight important data published in the past 12 months which provide new information on nontyphoidal salmonellosis in Africa.
Recent reviews and clinical studies continue to emphasize the challenges of diagnosis and management of invasive nontyphoidal Salmonellae (...
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PMID: 20736739
PDF is available here.
PDF is available here.
New Microbiologica (33)4 2010
Abstract
The Vibrio genus contains a large number of closely related bacterial species differing, in some cases, less than 1% in 16S rRNA gene sequence. The present study evaluated the usefulness of toxR gene for phylogenetic and evolution analysis on Vibrio isolates of environmental or clinical origin belon...
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PMID: 21213595
PDF is available here.
PDF is available here.
Abstract
A Gram-negative, motile, non-spore-forming bacterial strain, designated MJ07(T), was isolated from a farm soil and was characterized to determine its taxonomic position by using a polyphasic approach. Comparative 16S rRNA gene sequence analysis showed that strain MJ07(T) belongs to the family Alcali...
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PMID: 19933592
PDF is available here.
PDF is available here.
Abstract
A Gram-negative, motile, non-spore-forming bacterial strain, designated HU1-GD12(T), was isolated from freshwater sediment. The strain was characterized by using a polyphasic approach in order to determine its taxonomic position. Comparative analysis of the 16S rRNA gene sequence showed that the iso...
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PMID: 19946046
PDF is available here.
PDF is available here.
Abstract
We propose the names Mucilaginibacter gossypii sp. nov. (type strain Gh-67(T) =NCIMB 14470(T) =KCTC 22380(T)) and Mucilaginibacter gossypiicola sp. nov. (type strain Gh-48(T) =NCIMB 14471(T) =KCTC 22379(T))....
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PMID: 19946048
PDF is available here.
PDF is available here.
Abstract
I and/or anteiso-C₁₇ :₁ B), iso-C₁₅:₀ and C₁₆:₁ω5c. The fatty acid content of strain 6424S-61(T) mainly comprised iso-C₁₅ :₁ G and iso-C₁₅:₀. Comparative analysis of phenotypic and phylogenetic traits indicated that strains 6424S-25(T) and 6515J-31(T) represented t...
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PMID: 19946049
PDF is available here.
PDF is available here.
Abstract
Bacterial strains belonging to DNA hybridization groups (HG) II, IV and V, in the Erwinia herbicola-Enterobacter agglomerans complex, of Brenner et al. [Int J Syst Bacteriol 34 (1984), 45-55] were suggested previously to belong to the genus Pantoea, but have never been formally described and classif...
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PMID: 19946052
PDF is available here.
PDF is available here.
Abstract
Two strains of anaerobic, Gram-stain-negative bacilli isolated from the human oral cavity (D033B-12-2(T) and D080A-01) were subjected to a comprehensive range of phenotypic and genotypic tests and were found to be distinct from any previously described species. 16S rRNA gene sequence analysis reveal...
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PMID: 19946051
PDF is available here.
PDF is available here.
Abstract
Three housekeeping genes (dnaK, groEL and rpoB) of strains belonging to the genus Gluconacetobacter (37 strains) or related taxa (38 strains) were sequenced. Reference strains of the 15 species of the genus Gluconacetobacter were included. Phylogenetic trees generated using these gene sequences conf...
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PMID: 19915110
PDF is available here.
PDF is available here.
Abstract
We propose the previously deposited reference strain ATCC 12670(T) =DSM 44181(T) =NCIMB 10420(T), located in collections worldwide, as the type strain....
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PMID: 19915104
PDF is available here.
PDF is available here.
Abstract
These results and the differentiating biochemical and chemotaxonomic properties show that strain R4-1A(T) represents a novel species, for which the name Chryseobacterium culicis sp. nov. is proposed. The type strain is R4-1A(T) (=LMG 25442(T) =CCM 7716(T))....
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PMID: 19933582
PDF is available here.
PDF is available here.
Abstract
A marine bacterial strain, designated fav-2-10-05(T), was isolated from the mucus layer of a coral of the genus Favia, collected from the coral reef in the Gulf of Eilat, Israel (29.5 ° N 34.9 ° E). On the basis of 16S rRNA gene sequence comparisons, strain fav-2-10-05(T) was affiliated with t...
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PMID: 19915103
PDF is available here.
PDF is available here.
Abstract
A polyphasic taxonomic study of a halotolerant bacterium, isolated from sandy rhizospheric soil in Sarbandar, Persian Gulf, Iran, revealed that strain HM6(T) represents a novel species within the genus Nocardiopsis. Results of the 16S rRNA gene sequence comparison revealed that strain HM6(T) cluster...
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PMID: 19933587
PDF is available here.
PDF is available here.
Abstract
Three strains of Gram-negative, rod-shaped, non-spore-forming bacteria (M 2040(T), M 1973 and M 1878-SK2), isolated from milk of camels at a camel-milk production farm in the United Arab Emirates, were investigated for their taxonomic allocation. On the basis of 16S rRNA gene sequence similarities,...
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PMID: 19933583
PDF is available here.
PDF is available here.
Abstract
A Gram-positive, endospore-forming, rod-shaped bacterium, strain PD-A10(T), was isolated from salted crab (poo-khem) in Thailand and subjected to a taxonomic study. Phenotypic and chemotaxonomic characteristics, including phylogenetic analyses, showed that the novel strain was a member of the genus...
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PMID: 19933584
PDF is available here.
PDF is available here.
Abstract
A bacterial strain, SPC26(T), was isolated from a sediment sample of the Southern Ocean off Antarctica. The strain was Gram-staining- and catalase-positive and contained lysine and alanine in the cell-wall peptidoglycan. The major cellular fatty acids were anteiso-C₁₅:₀ (54.92 %), iso-C₁...
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PMID: 19783612
PDF is available here.
PDF is available here.
Abstract
These results suggested that strain VN05A0561(T) represents a novel species of the genus Pseudonocardia, for which the name Pseudonocardia babensis sp. nov. is proposed. The type strain is VN05A0561(T) (=VTCC-A-1757(T)=NBRC 105793(T))....
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PMID: 19933589
PDF is available here.
PDF is available here.