Find your PDFs fast.
Welcome to the Pubget Medical Subject Headings (MESH) browser. Click on a topic or subtopic below to explore related papers. In the gold box is the most recent paper about the current MESH term, and below are 20 related papers.
Limulus Test (0):
Recent article
Limulus Test
Abstract
We describe a patient presenting with colonic perforation who recovered from septic shock with DHP-PMX. This treatment effect was reflected by a decrease in plasma endotoxin levels as demonstrated more readily with endotoxin scattering photometry assay than with the standard limulus test. We conclud...
|
PMID: 20453426
PDF is available here.
PDF is available here.
| < More recent | Older article > |
Bioscience Reports (30)1 2010
Abstract
We screened 78 herbs based on biosensor technology by targeting of lipid A. Terminaliachebula Retz was found to possess the highest capability of binding lipid A. With CER (cation-exchange resin) and HPLC, we obtained three active components extracted from Terminaliachebula Retz, and named them TCR1...
|
PMID: 19203350
PDF is available here.
PDF is available here.
Abstract
Radiopharmaceuticals play an important role in modern nuclear medicine, and 18F-FDG (2-[18F] fluoro-2-deoxy-D-glucose) is the most frequently used in imaging examinations today. The pyrogen test represents an important parameter for the quality of radiopharmaceuticals since most of them, 18F-FDG inc...
|
PMID: 21140657
PDF is available here.
PDF is available here.
Abstract
There is a need for new microbicidal agents with therapeutic potential due to antibiotic resistance in bacteria and fungi. In this study, the structure-microbicidal activity relationship of amino acid residues 14 to 31 (sequence 14-31) from the N-terminal end, corresponding to the antibacterial alph...
|
PMID: 19917761
PDF is available here.
PDF is available here.
Abstract
The results demonstrate the presence of serovar-specific LPS in AH, and thus it is likely that endotoxin is a causative factor in leptospiral uveitis....
|
PMID: 18658094
PDF is available here.
PDF is available here.
Abstract
The median LPS concentrations were dramatically higher among patients who had symptoms of BV compared to those who did not have symptoms (3235.0 vs 46.4 EU/ml, respectively, P<0.001); patients who had BV also had much higher colonization densities of P. bivia (0.06+/-0.36 vs 5.4+/-2.2 log(10) CFU/ml...
|
PMID: 18849004
PDF is available here.
PDF is available here.
Abstract
We assayed a new-generation filter which is able to capture endotoxins from solutions....
|
PMID: 18723658
PDF is available here.
PDF is available here.
Abstract
(1-->3)-beta-D-glucan is found in cell walls of some fungi, bacteria and plants. It plays a crucial role in bioaerosol-induced inflammatory reactions. To estimate the level of airborne (1-->3)-beta-D-glucan exposure, a monoclonal antibody-based two-site enzyme immunoassay (mAb-EIA) was developed. Th...
|
PMID: 18589436
PDF is available here.
PDF is available here.
Abstract
The cytokine of IFN-gamma became detectable on day 7 and its peak value appeared on day 14 by ELISPOT. The CTL was detected on day 7 and the maximum lysis of CTL appeared on day 28. The cellular immune response of IFN-gamma of MNCs were significantly correlated with the doses vaccinated from 1 micro...
|
PMID: 19031766
PDF is available here.
PDF is available here.
Abstract
We synthesized a cyclic peptide (CLP-19), and then investigated its bioactivity both in vitro and in vivo. The ability of CLP-19 to neutralize LPS in vitro was tested using a Limulus amebocyte lysate (LAL) assay and the LPS-binding affinity was measured with an affinity biosensor method. The synthet...
|
PMID: 18442780
PDF is available here.
PDF is available here.
Abstract
We recommend extraction in PFW-Tween combined with dilution and LAL analysis in PFW....
|
PMID: 18441112
PDF is available here.
PDF is available here.
Abstract
The aim of this study was to evaluate the effectiveness of ozonated water in the elimination of Candida albicans, Enterococcus faecalis, and endotoxins from root canals. Twenty-four single-rooted human teeth were inoculated with C. albicans and E. faecalis, and 24 specimens were inoculated with Esch...
|
PMID: 18280954
PDF is available here.
PDF is available here.
Journal of Endodontics (34)3 2008
Abstract
Our findings indicated that chemomechanical preparation with 2.5% NaOCl was moderately effective against bacteria but less effective against endotoxins in root canal infection. Furthermore, a statistically significant association was found between higher levels and clinical symptomatology....
|
PMID: 18291273
PDF is available here.
PDF is available here.
Abstract
These results provide important background information for design of future laboratory or animal experiments, as well as for interpretation of field measurement data....
|
PMID: 18065630
PDF is available here.
PDF is available here.
Cytotherapy (10)4 2008
Abstract
Valid paired results, based on each center's LAL method and the Endosafe system criteria, were analyzed. Endotoxin detection between paired results was equivalent in most cases. DISCUSSION: The Endosafe system provided reliable results with products typically produced in cell-therapy manufacturing f...
|
PMID: 18574775
PDF is available here.
PDF is available here.
Abstract
We used the Limulus amoebocyte lysate assay to screen a broad range of commercial- and pharmaceutical-grade proteins, peptides, lipids, and inhibitors commonly used in microglia research for contamination with LPS. We then characterized the ability of PMX to alter a representative set of factor-indu...
|
PMID: 17910052
PDF is available here.
PDF is available here.
Abstract
Air samples were collected on glass fibre filters in 22 animal houses and 3 hay storage barns and examined for the presence of bacterial endotoxin with the Limulus (LAL) test and the gas chromatography-tandem mass spectrometry (GC-MSMS) technique, based on detection of 3-hydroxy fatty acids (3-OH-FA...
|
PMID: 18247467
PDF is available here.
PDF is available here.
Journal of Medical Microbiology (56)Pt 11 2007
Abstract
These results indicate that S. marcescens possesses a penta-acylated lipid A, which is nearly identical to E. coli lipid A in regard to biological activities, while it also may be a crucial virulence factor of the bacterium....
|
PMID: 17965342
PDF is available here.
PDF is available here.
Journal of Asthma (44)8 2007
Abstract
Beta-(1,3)-glucan is pro-inflammatory and has been associated with airway inflammation and respiratory symptoms. This study assessed beta-(1,3)-glucan levels from upper body clothing (jerseys) of 55 subjects. Beta-(1,3)-glucan levels were estimated with a modified Limulusamoebocyte lysate kinetic as...
|
PMID: 17943576
PDF is available here.
PDF is available here.
Abstract
Limulus amebocyte lysate (LAL) assay and gas chromatography-mass spectrum (GC-MS) analysis are usually used for the quantification of lipopolysaccharides (LPS) from the environment. The LAL assay measures endotoxin units to represent the LPS biological function but GC-MS analysis measures decanoic (...
|
PMID: 17188811
PDF is available here.
PDF is available here.
Abstract
Serum ET levels significantly increased during 6-72 h period after PH compared with NC and SO groups, and there were two peak values at 12 and 48 h while serum DAO level significantly increased at 12 and 24 h. There was positive correlation (r = 0.757, P < 0.05) between the levels of DAO and ET dyna...
|
PMID: 17659709
PDF is available here.
PDF is available here.
Abstract
PMB neutralizes LPS and inhibites the expression and release of cycokines in macrophages, in which the affinity of PMB for lipid A plays an important role....
|
PMID: 17535644
PDF is available here.
PDF is available here.
Journal of Infectious Diseases (195)11 2007
Abstract
These results suggest the secreted polysaccharide beta -1,3 glucan may serve as a useful tool for the diagnosis of Candida biofilm and device-associated infections....
|
PMID: 17471441
PDF is available here.
PDF is available here.
Abstract
The F1-V vaccine antigen, protective against Yersinia pestis, exhibits a strong tendency to multimerize that affects larger-scale manufacture and characterization. In this work, the sole F1-V cysteine was replaced with serine by site-directed mutagenesis for characterization of F1-V non-covalent mul...
|
PMID: 17293124
PDF is available here.
PDF is available here.
Early effects triggered by Larrea divaricata Cav. on murine macrophages at apoptotic concentrations.
Abstract
Decoction and infusion of Larrea divaricata were tested at apoptotic concentrations (1 and 4 mg/ml) on peritoneal murine macrophages. Consistent changes were observed after incubation with 4 mg/ml decoction. Phagocytosis of zymosan, lysosomal enzyme activity, nitric oxide production, TNF-alpha relea...
|
PMID: 18075869
PDF is available here.
PDF is available here.
Abstract
A comparative bench-scale study of endotoxin contamination is presented for two common processes of immunoglobulin purification from equine plasma: ammonium sulphate fractionation of F(ab')2 fragments and caprylic acid precipitation of non-IgG proteins. To this end, both processes were carried out u...
|
PMID: 18047176
PDF is available here.
PDF is available here.
Medicina (Kaunas, Lithuania) (43 Suppl 1) 2007
Abstract
The level of endotoxins was less than 0.25 endotoxin unit (EU)/mL in all hemodialysis water (n=6) and dialysate (n=14) samples. One sample of water (16.7%) and two of dialysate (14.3%) had the level of endotoxins above 0.03 EU/mL. Therefore, the level of endotoxins in hemodialysis water and dialysat...
|
PMID: 17551282
PDF is available here.
PDF is available here.
Cardiovascular Research (73)1 2007
Abstract
Interleukin-8 secretion, monocyte adhesion, and E-selectin expression were measured in human umbilical vein endothelial cells (HUVECs) and coronary artery endothelial cells (HCAECs) challenged in vitro with highly purified endotoxins of common host colonisers Escherichia coli, Porphyromonas gingival...
|
PMID: 17156768
PDF is available here.
PDF is available here.
Abstract
Abstract
We designed and synthesized lipid A analogues containing acidic amino acid residues in place of the non-reducing end phosphorylated glucosamine. Definite switching of the endotoxic or antagonistic activity was observed depending on the difference of the acidic groups (phosphoric acid or carboxylic a...
|
PMID: 16828560
PDF is available here.
PDF is available here.
Abstract
We have prepared conjugates of polymyxin B (PMB) and the cytokine binding protein alpha2-macroglobulin (A2M). The conjugate binds TNF-alpha as well as LPS as studied by electrophoresis and phase partitioning. Compared with free PMB, the conjugate is nontoxic to cells and does not affect the viabilit...
|
PMID: 16705081
PDF is available here.
PDF is available here.
Abstract
We used peptides, derived from the binding regions of known LPS-binding proteins, as scaffolds to introduce modifications at the amino acid level. Structure-activity relationship studies have shown that these modifications generate highly active peptides. Thus, from a bioactive peptide with an initi...
|
PMID: 16625684
PDF is available here.
PDF is available here.
Abstract
This paper presents a rapid, highly-sensitive, and low-cost method of endotoxin quantification based on the use of stress-responsive magnetoelastic sensors, that monitor the gel formation (viscosity change) of the Limulus Amoebocyte Lysate (LAL) assay in response to endotoxin. Ribbon-like magnetoela...
|
PMID: 16356707
PDF is available here.
PDF is available here.
Abstract
In analyzed synovial fluids, suspected for endotoxin presence, the presence of endotoxin done was proved by LAL-test in 21 synovial fluids /42%/ and in 12 of 20 /60%/ the presence of endotoxin inhibiting substances /IS/ was proved. Because many parameters /like presence CIC, anti-LPS, KDO/ indicates...
|
PMID: 17195360
PDF is available here.
PDF is available here.
Abstract
Inhalation of bioaerosols from animal houses can induce acute inflammatory reactions in the respiratory tract. Determination of the concentration of airborne endotoxins is widely used to characterize this risk. In this study, the activity of bioaerosol samples from a duck-fattening unit to induce in...
|
PMID: 16629985
PDF is available here.
PDF is available here.
Abstract
The spike recovery was 113.63% (95% CI = 101.69, 125.57%) for LAL and 99.69% (95% CI = 90.14, 109.24%) for rFC assays. The LAL assay gave higher endotoxin estimates compared with rFC. The LAL and rFC estimates were highly correlated (r = 0.86, P < 0.0001). The difference between LAL and rFC endotoxi...
|
PMID: 16550568
PDF is available here.
PDF is available here.
Journal of Endodontics (32)4 2006
Abstract
Journal of Endodontics (32)4 2006
Abstract
Fluid was aspirated from the root canals of 40 endodontically involved teeth. This fluid was assayed for endotoxin with the limulus lysate test. Pulpless teeth contained greater concentrations of endotoxin than those with vital pulps. Symptomatic teeth also contained more endotoxin than asymptomatic...
|
PMID: 16554197
PDF is available here.
PDF is available here.
Abstract
Data from human inhalation studies have increased our understanding of the cell mechanisms underlying diseases related to endotoxin exposure. In addition, knowledge from molecular genetics may help us to identify individuals at risk. Several investigations have demonstrated that, apart from endotoxi...
|
PMID: 16505614
PDF is available here.
PDF is available here.
Abstract
We compared the immune stimulatory potency of 11 different LPSs using human whole blood incubations. While the majority of LPSs induced cytokine release equipotently, a 1,000-fold more LPS from Pseudomonas aeruginosa or Vibrio cholerae was still less potent in inducing TNF, IL-1 beta, IL-10 and IFN-...
|
PMID: 16719988
PDF is available here.
PDF is available here.
Abstract
beta-Glucans are present in standardized allergen extracts. The effects of these quantities of beta-glucans on allergen immunotherapy and allergen skin testing require further study....
|
PMID: 16953976
PDF is available here.
PDF is available here.
FEMS Microbiology Letters (79)2-3 2006
Abstract
I variants of B. pertussis, with the exception of strain 134, contain a preponderance of LPS I whereas the major component of LPS of phase IV variants in LPS II. Sera raised to LPSs of phase I strains, other than 134, cross-react with each other but not with phase IV LPSs; and similarly all sera rai...
|
PMID: 2060761
PDF is available here.
PDF is available here.
FEMS Microbiology Letters (137)1 2006
Abstract
Endotoxic activities of lipopolysaccharide (LPS) isolated from Burkholderia (Pseudomonas) pseudomallei, a causative agent of melioidosis, were investigated. Compared to an enterobacterial LPS (SAE-LPS), B. pseudomallei LPS (BP-LPS) exhibited weaker pyrogenic activity in rabbits, lethal toxicity in ga...
|
PMID: 8935661
PDF is available here.
PDF is available here.
Abstract
We undertook this study to determine if lipopolysaccharide (LPS) extraction efficiency could account for differences among laboratories. We generated and collected aerosols from chicken and swine barns, and corn processing. We randomly allocated side-by-side filter samples to five laboratories for L...
|
PMID: 16307099
PDF is available here.
PDF is available here.
Medicinal Chemistry (1)6 2005
Abstract
We have studied the interaction of PhoE with the membrane-forming lipids phosphatidylethanolamine (PE) and phosphatidylglycerol (PG) from the inner and lipopolysaccharide (LPS) from the outer leaflet of the outer membrane. These investigations comprise functional aspects of the protein:lipid interac...
|
PMID: 16787338
PDF is available here.
PDF is available here.
Abstract
We evaluated room-specific within-home and between-home variability in dust endotoxin obtained from 470 households in Boston, Massachusetts. Homes were sampled up to two times over 5-11 months. We analyzed 1,287 dust samples from the kitchen, family room, and baby's bedroom for endotoxin. We fit a m...
|
PMID: 16263505
PDF is available here.
PDF is available here.
Abstract
In the study of undiluted FDG, seven of 50 PPC vials at 12 EU x ml(-1) did not gel. The lowest endotoxin concentration that consistently gelled at 20 min was 4 EU x ml(-1) . In the sample tested for inhibition using PPC tubes at 4 EU x ml(-1) , 49 tubes gelled at 20 min, and one did not. CONCLUSION:...
|
PMID: 16160645
PDF is available here.
PDF is available here.
Abstract
The usefulness of LAL test in routine diagnostic procedures in hospitalized children with infections was not proven so far. However, the LAL test appeared useful in the urinary tract infection (UTI) cases in which significant elevation of serum ET levels were demonstrated. Moreover, it was confirmed...
|
PMID: 16245427
PDF is available here.
PDF is available here.
Nippon rinsho. Japanese journal of clinical medicine (63 Suppl 7) 2005
Abstract
Artificial Organs (29)6 2005
Abstract
We demonstrated a semilogarithmic model in which the optical densities measured after the ELISA assay increases linearly with the levels of endotoxin. This model allowed the determination of the amount of endotoxins in biofilm samples with a detection limit of 0.032 EU/mL. Most of the time, the amou...
|
PMID: 15926985
PDF is available here.
PDF is available here.
Abstract
We have earlier found that a trivalent vaccine, containing antigenic components from both Gram-positive and Gram-negative bacteria, induced secretion of the endogenous pyrogen interleukin 6 (IL-6) when added to fresh human blood in vitro. The results of the present study showed that the IL-6 secreti...
|
PMID: 15882532
PDF is available here.
PDF is available here.
Yonsei Medical Journal (46)2 2005
Abstract
We propose a possible new method for detecting endotoxin using a pH-indication system of macrophage culture media. After RAW 264.7 macrophage cells were treated with lipopolysaccharide (LPS), the addition of fluorescein to the LPS-treated media reproductively reduced its absorption and emission spec...
|
PMID: 15861501
PDF is available here.
PDF is available here.
Abstract
We detected non-specific reactions in the measurement of (1-->3)-beta-D-glucan levels (beta-glucan) in plasma, and the influences of the non-specific reactions on sensitivity and specificity of measurement methods were examined. In this study, 460 plasma samples from 174 patients at Kawasaki Medical...
|
PMID: 15977573
PDF is available here.
PDF is available here.
Abstract
To investigate the efficacy of pexiganan, a 22-residue magainin analog, alone and combined with betalactmas antibiotics in three experimental rat models of Gram-negative septic shock. Adult male Wistar rats were given (i) an intraperitoneal injection of 1 mg Escherichia coli 0111:B4 LPS; (ii) 2x10(1...
|
PMID: 15629532
PDF is available here.
PDF is available here.
Abstract
We report the construction, expression and purification of a bifunctional protein, hIL-2/GM-CSF, which may facilitate interaction between T cells and the antigen presentation cells and improve the efficiency of antigen presentation. We found that the use of chemicals and temperature shift is a pecul...
|
PMID: 15642462
PDF is available here.
PDF is available here.
Journal of Dentistry (33)2 2005
Abstract
The calcium hydroxide and polymyxin B intracanal medicaments detoxified endotoxin in root canals and altered the properties of LPS to stimulate the antibody production by B-lymphocytes. The combination neomycin-polymyxin B-hydrocortisone did not detoxified endotoxin....
|
PMID: 15683891
PDF is available here.
PDF is available here.
Abstract
The aim of the study was to determine bacterial endotoxin concentration in the water flowing from a high-speed handpiece of a dental unit and in the air contained in the bioaerosol formed during dental conservative treatment. The air was collected in the space between the patient and dentist. The st...
|
PMID: 16028879
PDF is available here.
PDF is available here.
Human Vaccines (1)4 2005
Abstract
We attempt to explore a test system for the laboratory safety evaluation of CyaA preparations. Endotoxin was determined in vitro by the Limulus amoebocyte lysate assay. Cytotoxicity was measured by a tetrazolium salt test and a lactate dehydrogenase release assay using murine and human phagocytic ce...
|
PMID: 17012877
PDF is available here.
PDF is available here.
Abstract
We have focused, in the present study, on the effects of the acyl moieties and acidic groups at the 1- and 4'- positions. We synthesized a new analogue corresponding to Rubrivivax gelatinosus lipid A, which has a characteristic symmetrical distribution of acyl groups on the two glucosamine residues...
|
PMID: 16303089
PDF is available here.
PDF is available here.
Igiene e sanita pubblica (61)5 2005
Abstract
In 1995 the regional council of the Lazio region passed Decree N.1650 which regulates the maintenance of dialysis equipment and sets limiting values for microbiological and chemical parameters used to assess the quality of water used for dialysis fluids. As regards the microbiological parameters, li...
|
PMID: 17206217
PDF is available here.
PDF is available here.