Abstract
Non-faecalis and non-faecium Enterococcus species can cause protean manifestations which vary with the infecting Enterococcus species. Misidentification of unusual enterococcal species might occur by the commercial identification methods and accurate identification with molecular methods is required...
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PMID: 20433865
PDF is available here.
Abstract
We have developed a rapid and cost-efficient strategy that combines cell-free (CF) protein synthesis, optimized combinatorial dual-isotope labeling for nearly instant resonance assignment, and fast acquisition of long-distance information using paramagnetic probes. Here we report three backbone stru...
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PMID: 20498088
PDF is available here.
Abstract
Water samples of ten mineral water springs at Miercurea Ciuc (Csíkszereda) region (Romania) were examined during 2005-2006 using cultivation-dependent microbiological methods. The results of standard hygienic bacteriological tests showed that the Hargita Spring had perfect and five other springs ha...
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PMID: 20587384
PDF is available here.
Abstract
We present a severe case of acute tonsillitis caused by Borrelia vincenti. B. vincenti does not only cause Plaut-Vincent's angina, but also a more localised infection of the tonsil.
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PMID: 20237790
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Abstract
Staphylococcus spp. are microorganisms that are naturally present in milk and dairy products and are often associated with food-borne diseases outbreaks due to the ability of some strains to produce thermostable enterotoxins. This ability is usually associated with coagulase and thermonuclease produ...
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PMID: 20417392
PDF is available here.
Abstract
We investigated the relationship between the time from obtaining the blood specimen to the detection of positive culture tests as well as the significance of the pathogen detected. Twenty-three inpatients demonstrated CNS-positive blood culture tests. It was judged that six patients (26%) had contam...
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PMID: 20622485
PDF is available here.
Abstract
Though the Koch's bacillus was discovered 128 years ago, still there lies some dilemma in its diagnosis in certain clinical situations. Here in this article two basic approaches have been described for its diagnosis. One is the 'direct' approach in which detection of mycobacteria or its products is...
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PMID: 21121388
PDF is available here.
Abstract
We evaluated the Uro4 HB&L automated system for the rapid diagnosis of respiratory tract infections in ICU patients. A total of 644 lower respiratory tract specimens collected from 400 inpatients from nine ICUs at the Padova University hospital were collected during a 12-month period. All samples we...
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PMID: 20347888
PDF is available here.
Abstract
Nasopharyngeal carriage studies are needed to monitor changes in important bacterial pathogens in response to vaccination and antibiotics. Commercial swab transport followed by transfer to skim milk tryptone glucose glycerol broth for frozen storage is an option for studies of Streptococcus pneumoni...
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PMID: 20298727
PDF is available here.
Motoki Goto,
Kayo Shimada,
Ayako Sato,
Eri Takahashi,
Takafumi Fukasawa,
Tomoki Takahashi,
Seii Ohka,
Takahide Taniguchi,
Eiichi Honda,
Akio Nomoto,
Atsuo Ogura,
Teruo Kirikae and
Ken-Ichi Hanaki
Abstract
A colorimetric loop-mediated isothermal amplification (LAMP) assay with hydroxy naphthol blue was designed to amplify a region in the outer membrane lipoprotein (oprL) gene of Pseudomonas aeruginosa. The LAMP assay showed 100% specificity for the serogroup and other bacteria, and the sensitivity was...
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PMID: 20298724
PDF is available here.
Abstract
We investigated the time gain after the introduction of a molecular assay and compared this with a preceding control period, using culture-based techniques. During a four-month period all high risk patients (N=44) and all known MRSA-positive patients readmitted to the hospital (N=41) were screened f...
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PMID: 20381915
PDF is available here.
Abstract
A quadruplex real-time (RT) qPCR assay for the detection and quantification in 4h of Staphylococcusaureus, Pseudomonasaeruginosa, Acinetobacterbaumannii and Stenotrophomonasmaltophilia directly from bronchoalveolar lavage specimens was developed. The specificity of the assay was 100% for all four sp...
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PMID: 20353803
PDF is available here.
Abstract
Contagious bovine pleuropneumonia (CBPP) is the most serious cattle disease in Africa, caused by Mycoplasma mycoides subsp. mycoides small-colony type (SC). CBPP control strategies currently rely on vaccination with a vaccine based on live attenuated strains of the organism. Recently, an lppQ(-) mut...
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PMID: 20381545
PDF is available here.
Abstract
Bartonella henselae is the causative agent of cat scratch disease, a human infection usually characterized by persistent regional lymphadenopathy. It is transmitted to humans by cat scratches or bites. Cats are the major reservoir for this bacterium thus B. henselae has a worldwide distribution. The...
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PMID: 20042306
PDF is available here.
Abstract
Roseiflexus sp. strains were cultivated from a microbial mat of an alkaline siliceous hot spring in Yellowstone National Park. These strains are closely related to predominant filamentous anoxygenic phototrophs found in the mat, as judged by the similarity of small-subunit rRNA, lipid distributions,...
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PMID: 20363941
PDF is available here.
Abstract
P. aeruginosa induces keratitis in this lens-wearing model after a single inoculation. Delayed disease onset was interesting considering the greater keratitis risk during extended wear. Infection did not require the disruption of corneal barrier function before lens wear and occurred without exposur...
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PMID: 20130275
PDF is available here.
Abstract
The disruption of intestinal barrier leads to the penetration of noxious luminal compounds into the gut wall, causing further damage. This unit describes the assessment of enteric bacteria translocation into the intestinal wall of rats, an established method for the evaluation of bow...
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PMID: 20967745
PDF is available here.
Abstract
Streptococcus (54.5%), Neisseria (14.7%), Actinomyces (8.4%), Gemella (4.1%), Granulicatella (3.8%), and Prevotella (1.4%) were dominant in a total of 1655 clones examined from the saliva of the healthy subjects. The dominant genera differed among the patients with odontogenic infections (a total of...
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PMID: 20171911
PDF is available here.
Abstract
ESBL organisms provide a continuing challenge in the geriatric community. They are increasingly prevalent, and pose unique challenges in treatment. Carbapenems are the mainstay of therapy; however they are expensive medications that require prolonged intravenous administration. Carbapenem resistance...
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PMID: 20533728
PDF is available here.
Abstract
Many filamentous fungi secrete considerable quantities of enzymes including protease, cellulase and xylanase, which are of major industrial importance. Over the past few decades, many of these fungal enzymes have been isolated and their relevant genes characterised. Solid-state fermentation (SSF), a...
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PMID: 20145973
PDF is available here.