Abstract
To determine simple laboratory test in children with functional abdominal pain, to compare this test in those with functional abdominal pain in normal children and to try to search for it cause if any, and to determine the symptoms and signs of the disease.
This study...
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PMID: 21894358
PDF is available here.
Abstract
The French National reference Laboratory for Human papillomavirus (HPV) performed in 2009 a national study in order to review the methods used to detect and identify HR HPV genotypes in microbiology laboratories. Results from this study show a great diversity in volumes of samples tr...
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PMID: 21659046
PDF is available here.
Abstract
As a part of a series of yearly meeting, in May 2010 over 40 medical laboratory opinion leaders, pathologists, clinical biochemists and physicians from Europe, Israel and South Africa gathered together in Bardolino, Italy to discuss issues and current challenges for laboratory medicine, including a)...
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PMID: 21366504
PDF is available here.
Abstract
We conducted an age-matched control study comparing children hospitalized for pH1N1 with historic controls infected with seasonal H1N1 and H3N2 influenza to correct for the effect of age on disease susceptibility and clinical manifestations. We also compared children with pH1N1 to children concurren...
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PMID: 21799750
PDF is available here.
Abstract
This document affirms as final, with changes, a proposed rule that updates the Department of Veterans Affairs (VA) medical regulations concerning the payment methodology used to calculate VA payments for inpatient and outpatient health care professional services and other medical services associated...
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PMID: 21166263
PDF is available here.
Abstract
Diagnosis of Strongyloides stercoralis is often delayed owing to patients presenting with nonspecific gastrointestinal complaints, a low parasite load and irregular larval output. Although several diagnostic methods exist to detect the presence of S. stercoralis there is no gold standard. In immunoc...
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PMID: 20733481
PDF is available here.
Abstract
To update the reader on the latest developments in the laboratory diagnosis of intestinal protozoa.
Correct identification of a diarrhoea causing pathogens is essential for the choice of treatment in an individual patient as well as to map the aetiology of diarrhoea in a variety of patient populatio...
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PMID: 20683263
PDF is available here.
Abstract
A clinical and laboratory studies were undertaken to evaluate the efficiency of perioperative parenteral nutrition in destructive forms of acute calculous cholecystitis. Based on the general and biochemical blood analyses and the time course of changes in humoral immunological parameters, the author...
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PMID: 21162181
PDF is available here.
D Cohen,
N Gargouri,
A Ramlawi,
Z Abdeen,
A Belbesi,
B Al Hijawi,
A Haddadin,
S Sheikh Ali,
N Al Shuaibi,
R Bassal,
R Yishai,
M S Green and
A Leventhal
Abstract
In late 2002, health professionals from the ministries of health and academia of Jordan, the Palestinian Authority and Israel formed the Middle East Consortium on Infectious Disease Surveillance (MECIDS) to facilitate trans-border cooperation in response to infectious disease outbreaks. The first mi...
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PMID: 20158930
PDF is available here.
Abstract
The disease pyramid of under-ascertainment for surveillance of acute gastrointestinal illness (AGI) in New Zealand has been estimated using 2005-2007 data on notifiable diseases, a community telephone survey, and a survey of diagnostic laboratories. For each notified case of AGI there were an estima...
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PMID: 20196904
PDF is available here.
Abstract
More uniform core sizes are obtained with the homopolymer human H-chain ferritin than with the heteropolymer horse spleen HoSF protein in which subpopulations of HoSF molecules with varying iron content are observed. A binomial probability distribution of H- and L-subunits among protein shells quali...
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PMID: 20307627
PDF is available here.
Shinobu Umemura,
Masafumi Kurosumi,
Takuya Moriya,
Tetsunari Oyama,
Kohji Arihiro,
Hiroko Yamashita,
Yoshihisa Umekita,
Yoshifumi Komoike,
Chikako Shimizu,
Hisaki Fukushima,
Hiroshi Kajiwara,
Futoshi Akiyama and
Japanese Breast Cancer Society
Abstract
We summarize the evidence, on which the recommendations were made, and customize the recommendations suitable for the current status....
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PMID: 20596613
PDF is available here.
Abstract
To assess the test quality of HBsAg, anti-HBc IgM and anti-HAV IgM in the laboratories of Hepatitis B pilot surveillance provinces.
Blood serum from each of the Hepatitis B pilot surveillance provinces were collected to verify the test results. The Chemiluminescence Microparticle Immuno Assay (CMIA)...
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PMID: 20726257
PDF is available here.
Abstract
T-Cell-Xtend-independent median ELISPOT counts (spot forming cells per million peripheral blood mononuclear cells) were significantly higher at day 1 versus day 0 (114 vs. 100; n=66; p=0.03); inter-time-point agreement between the results was 95.45% and the conversion/reversion rate was 4.55%. By co...
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PMID: 20152856
PDF is available here.
Melissa J MJ Whaley,
Charles C Rose,
Joseph J Martinez,
Gouri G Laher,
Deborah L DL Sammons,
Jerry P JP Smith,
John E JE Snawder,
Ray R Borrow,
Raymond E RE Biagini,
Brian B Plikaytis,
George M GM Carlone and
Sandra S Romero-Steiner
Abstract
We compared three bead-based immunoassays: a commercial assay (xMAP Pneumo14; Luminex) and two in-house assays (of the Health Protection Agency [HPA] and Centers for Disease Control and Prevention [CDC]), using the WHO-recommended standard reference and reference sera (n = 11) from vaccinated adults...
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PMID: 20335434
PDF is available here.
Abstract
During a Histoplasma outbreak in a colony of fruit bats at a southern United States zoo, it was observed that although Histoplasma was recovered in culture from multiple sites at necropsy, none of the samples collected from those bats tested positive for Histoplasma antigen (HAg). Five of the Histop...
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PMID: 20357054
PDF is available here.
Abstract
We discuss the causes of these variations, and present a global quality control program to overcome them....
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PMID: 20201744
PDF is available here.
Abstract
Due to their complex preanalytics coagulation tests show a higher rate of rejected samples due to insufficient quality and a higher intra- and inter-individual test variability. In the last years several guidelines addressed this issue in an effort to standardize preanalytic procedures. However, in...
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PMID: 20454751
PDF is available here.
Abstract
The first TM EQA results of 2008 showed that the pass percentage of all participating laboratories ranged from 87.3% (CA125) to 95.5% (beta-2-microglobulin). And the second batch ranged from 83.5% (HCG) to 94.0% (beta-2-microglobulin). The coefficient variances (CVs) of intra-group values determined...
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PMID: 20646652
PDF is available here.
Abstract
Enterovirus 71 (EV71) is the main etiologic agent of hand, foot, and mouth disease (HFMD) and causes frequently severe neurological complications and mortality in young children. The serum neutralizing antibody response is the major indicator of EV71 infection and protective immunity. The current se...
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PMID: 20036286
PDF is available here.
Abstract
Since joining the Centers for Disease Control and Prevention's (CDC) Foodborne Diseases Active Surveillance Network (FoodNet) in 1999, Tennessee has conducted active surveillance for foodborne pathogens including Shiga toxin-producing E. coli (STEC). The number of STEC infections has increased in re...
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PMID: 20481249
PDF is available here.
Abstract
Laboratorians should be aware of the potential for carryover in random access analyzers, and should be mindful of appropriate troubleshooting techniques....
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PMID: 20148727
PDF is available here.
Kinue Ooi,
Kazushi Sugimoto,
Katsuya Shiraki,
Norihiko Yamamoto,
Masahiko Tameda,
Tetsuya Beppu,
Junichiro Tanaka,
Keiichiro Nojiri,
Satoko Kusagawa,
Yoshiyuki Takei,
Chiaki Masuda and
Tsutomu Nobori
Abstract
Patterns of hypocholesterolemic lipid fractions in 295 patients with liver diseases, malignant tumors, arteriosclerotic and renal diseases with cholesterol (Chol) levels of <30 mg/dl were classified using a simultaneous analytical method for the Chol and triglyceride (TG) fractions (Chol/Trig Combo...
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PMID: 20198309
PDF is available here.
Brian R BR Smith,
Maria M Aguero-Rosenfeld,
John J Anastasi,
Beverly B Baron,
Anders A Berg,
Jay L JL Bock,
Sheldon S Campbell,
Kendall P KP Crookston,
Robert R Fitzgerald,
Mark M Fung,
Richard R Haspel,
John Greg JG Howe,
Jeffrey J Jhang,
Malek M Kamoun,
Susan S Koethe,
Matthew D MD Krasowski,
Marie L ML Landry,
Marisa B MB Marques,
Henry M HM Rinder,
William W Roberts,
William E WE Schreiber,
Steven L SL Spitalnik,
Christopher A CA Tormey,
Paul P Wolf,
Yan Yun YY Wu and
Abstract
As the 100th anniversary of the Flexner report nears, medical student education is being reviewed at many levels. One area of concern, expressed in recent reports from some national health care organizations, is the adequacy of training in the discipline of laboratory medicine (also...
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PMID: 20231605
PDF is available here.
Abstract
A recombinant glycoprotein (R-GP) of vesicular stomatitis New Jersey virus (VSV-NJ) was expressed in insect cells by a baculovirus system. Its utility as a diagnostic antigen in a blocking ELISA was investigated as an alternative to the current native GP extracted from VSV-NJ. With the cut-off value...
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PMID: 20018211
PDF is available here.
Abstract
This Commentary provides a state of the art for array-based karyotyping in cancer diagnostics.
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PMID: 20075205
PDF is available here.
Abstract
Early laboratory diagnosis is critical for the optimal management of human malaria, particularly following the introduction of relatively expensive, artemisinin-combination therapies (ACT). The performance of the laboratories and the quality of malaria diagnosis have recently been assessed in 36 hea...
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PMID: 20406579
PDF is available here.
Abstract
We report a novel method using surface-enhanced laser desorption and ionization time of flight mass spectrometry to rapidly genotype, in a multiplex fashion, 3 SNPs that predispose patients to thrombosis. First, patient DNA samples were subjected to polymerase chain reaction to amplify and extend th...
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PMID: 20186013
PDF is available here.
Abstract
The T-SPOT.TB test does not cross-react with Bacille Calmette-Guérin or most non-tuberculosis mycobacterium species, and is based on IFN-gamma responses to Mycobacterium tuberculosis-specific antigens. The objective of this study was to compare tuberculin skin test (TST) with T-SPOT.TB results used...
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PMID: 20332570
PDF is available here.
Abstract
We propose several strategies for the post-analytical reporting of hemostasis assays, and how this may provide the final opportunity to prevent serious clinical errors in diagnosis. This report should be of interest to both the laboratory scientists working in hemostasis and clinicians that request...
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PMID: 20014957
PDF is available here.
A Sargent,
A Bailey,
A Turner,
M Almonte,
C Gilham,
H Baysson,
J Peto,
C Roberts,
C Thomson,
M Desai,
J Mather and
H Kitchener
Abstract
We present data on the use of the Hybrid Capture 2 (HC2) test for the detection of high-risk human papillomavirus (HR HPV) with different thresholds for positivity within a primary screening setting and as a method of triage for low-grade cytology. In the ARTISTIC population-based trial, 18,386 wome...
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PMID: 20007387
PDF is available here.
Abstract
We evaluated toxigenic Clostridium difficile detection by a lateral flow assay for antigen and toxin, an enzyme immunoassay, and two commercial PCR methods. Compared to the cell cytotoxicity neutralization assay and toxigenic culture, both toxin detection methods lacked sensitivity. PCR following co...
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PMID: 20032256
PDF is available here.
Abstract
We detected 109 parasitemias by real-time PCR and, by pooling samples, obviated over 50% of reactions and halved the cost of testing. Our study highlights both substantial discordance between malaria diagnostics and the utility and parsimony of employing a sample pooling strategy for molecular diagn...
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PMID: 19940051
PDF is available here.
Abstract
Previous studies have reported the increased sensitivity of PCR targeting AF146527 over that of PCR targeting the B1 gene for diagnosis of toxoplasmosis. The present study suggests that the AF146527 element was absent in 4.8% of human Toxoplasma gondii-positive samples tested. The data argue that th...
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PMID: 19940050
PDF is available here.
Alina Deshpande,
Jason Gans,
Steven W Graves,
Lance Green,
Laura Taylor,
Heung Bok Kim,
Yuliya A Kunde,
Pascale M Leonard,
Po-E Li,
Jacob Mark,
Jian Song,
Momchilo Vuyisich and
P Scott White
Abstract
We have developed a rapid (under 4 hours), multiplex, nucleic acid assay, adapted to a microsphere array detection platform. We call this assay multiplex oligonucleotide ligation-PCR (MOL-PCR). Unlike other ligation-based assays that require multiple steps, our protocol consists of a single tube rea...
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PMID: 20006656
PDF is available here.
Abstract
We demonstrated that physical disruption by bead-beating with 0.1mm beads in combination with MagNA Pure DNA III extraction enhances microbial lysis of diverse Gram-positive microorganisms and may be used to optimize DNA extraction protocols in routine clinical diagnostics.
Copyright 2009 Elsevier...
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PMID: 19995580
PDF is available here.
Abstract
The discrepancies between the two calibrations lead to significant clinical misinterpretation with decreased detection of PCa if tPSA cut-off thresholds are not adjusted....
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PMID: 20001849
PDF is available here.
Abstract
Prolactin results can be reported directly from the DxI. When results are discordant with the clinical presentation, prolactin should be measured using GFC....
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PMID: 19943807
PDF is available here.
Ephraim L Tsalik,
Daphne Jones,
Bradly Nicholson,
Lynette Waring,
Oliver Liesenfeld,
Lawrence P Park,
Seth W Glickman,
Lauren B Caram,
Raymond J Langley,
Jennifer C van Velkinburgh,
Charles B Cairns,
Emanuel P Rivers,
Ronny M Otero,
Stephen F Kingsmore,
Tahaniyat Lalani,
Vance G Fowler and
Christopher W Woods
Abstract
We identified patients from two large academic hospital emergency departments with suspected sepsis. The results of a multiplex PCR that could detect 25 bacterial and fungal pathogens were compared to those of blood culture. The results were analyzed with respect to the likelihood of infection, seps...
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PMID: 19846634
PDF is available here.
Abstract
We developed a new assay system which supports very high degrees of multiplexing. We developed a new class of mismatch-tolerant "sloppy" molecular beacons, modified them to provide an extended hybridization range, and developed a multiprobe, multimelting temperature (T(m)) signature approach to bact...
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PMID: 19923485
PDF is available here.
Abstract
Our two experts have been asked to consider the following question: what is the role of rapid immunochromatographic antigen testing in the laboratory diagnosis of influenza A virus infection during the current 2009 H1N1 pandemic?...
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PMID: 20007399
PDF is available here.
Abstract
The BD GeneOhm Cdiff assay, a real-time PCR assay for the detection of the Clostridium difficile toxin B (tcdB) gene, was compared with the toxin A/B (Tox A/B) II enzyme-linked immunosorbent assay (ELISA) and a two-step algorithm which includes a C. Diff Chek-60 glutamate dehydrogenase (GDH) antigen...
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PMID: 19864479
PDF is available here.