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Transfection (1):
Recent article
Transfection
Abstract
We demonstrate the utility of optogenetics to cardiac muscle by a tandem cell unit (TCU) strategy, in which nonexcitable cells carry exogenous light-sensitive ion channels, and, when electrically coupled to cardiomyocytes, produce optically excitable heart tissue. A stable channelrhodopsin2 (ChR2)-e...
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PMID: 21828312
PDF is available here.
PDF is available here.
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Abstract
We examined whether CETP affected the insulin-mediated responses in adipocytes....
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PMID: 21816162
PDF is available here.
PDF is available here.
Abstract
These results suggest that GABA(B)R is predominantly expressed by osteoblasts to negatively regulate osteoblastogenesis through down-regulation of BMP2 expression toward disturbance of osteoclastogenesis after down-regulation of RANKL expression in mouse bone....
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PMID: 21828041
PDF is available here.
PDF is available here.
Abstract
We demonstrate heightened susceptibility to thioglycollate-induced peritonitis in CD93(-/-) mice. CD93(-/-) mice showed a 1.6-1.8-fold increase in leukocyte infiltration during thioglycollate-induced peritonitis between 3 and 24 h that returned to wild type levels by 96 h. Impaired vascular integrit...
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PMID: 21849679
PDF is available here.
PDF is available here.
Brain research (1411) 2011
Abstract
We show MMP-9 overexpression conferred a poor prognosis in 163 GBM patients. Furthermore, MMP-9 specific miRNA expression profile (14 positively and 31 negatively correlated miRNAs with MMP-9) was established via miRNA microarrays in 60 GBM samples. Among them, two miRNAs: miR-885-5p and miR-491-5p,...
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PMID: 21831363
PDF is available here.
PDF is available here.
Abstract
We tested the hypothesis that activation of Nox1 NADPH oxidase modulates intracellular calcium ([Ca(2+)](i)) levels. Using cultured SMCs from wild-type and Nox1 null mice, we confirmed that thrombin-dependent generation of reactive oxygen species requires Nox1. Thrombin rapidly increased [Ca(2+)](i)...
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PMID: 21810651
PDF is available here.
PDF is available here.
Abstract
We demonstrated that changes in cell morphology are a key factor. Morphological manipulation of single cells without cell-cell contact resulted in flat spread or round compact cells with nuclear or cytoplasmic Yap, respectively. Stress fibers increased in response to expanded cell areas, and F-actin...
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PMID: 21831922
PDF is available here.
PDF is available here.
Abstract
We visually distinguished two sequential steps of secretagogue-stimulated exocytosis: fusion of individual secretory granules (Weibel-Palade bodies [WPBs]) and subsequent expulsion of von Willebrand factor (VWF) content. Based on our observations, we conclude that for fusion, WPBs are released from...
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PMID: 21844207
PDF is available here.
PDF is available here.
Abstract
We identify a specific subset of centrosomal proteins that are recruited to the cell cortex by DP upon epidermal differentiation. These include Lis1 and Ndel1, which are centrosomal proteins that regulate microtubule organization and anchoring in other cell types. This recruitment was mediated by a...
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PMID: 21844209
PDF is available here.
PDF is available here.
Abstract
We show that a phosphorylation gradient is produced by Aurora B concentration and activation at centromeres and release and diffusion to reach substrates at a distance. Kinase concentration, either at centromeres or at another chromosomal site, is necessary for activity globally. By experimentally m...
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PMID: 21844210
PDF is available here.
PDF is available here.
Abstract
We found that the ZA was converted to another type of AJ with punctate morphology (pAJ) at the margins of epithelial colonies. The F-actin-stabilizing protein EPLIN (epithelial protein lost in neoplasm), which functions to maintain the ZA via its association with αE-catenin, was lost in the pAJs. C...
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PMID: 21844208
PDF is available here.
PDF is available here.
Abstract
Our data suggest that the mTOR signaling pathway is involved in SPC-modulated melanin synthesis....
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PMID: 21838918
PDF is available here.
PDF is available here.
Developmental Cell (21)2 2011
Abstract
We conducted two genome-wide RNAi screens identifying genes required for intoxication and demonstrating that requirements are strikingly different between PE and Ricin, with only 13% overlap. Yet factors required by both toxins are present from the endosomes to the ER, and, at the morphological leve...
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PMID: 21782526
PDF is available here.
PDF is available here.
Abstract
To investigate new strategies to intensify chemosensitivity in head and neck squamous cell carcinoma.
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PMID: 21844414
PDF is available here.
PDF is available here.
Hormones and Cancer (2)4 2011
Abstract
We previously demonstrated that H2 relaxin (RLN2) facilitates castrate-resistant (CR) growth of prostate cancer (CaP) cells through PI3K/Akt/β-catenin-mediated activation of the androgen receptor (AR) pathway. As inhibition of this pathway caused only ~50% reduction in CR growth, the goal of the cu...
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PMID: 21789713
PDF is available here.
PDF is available here.
Abstract
We found that VPA potently induced hyperacetylation of histone H3 and H4, increased the expression of E-cadherin and inhibited cell migration in prostate cancer cells. Furthermore, knock-down of E-cadherin significantly restored the effects of VPA on cell migration, while over-expression of E-cadher...
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PMID: 21901986
PDF is available here.
PDF is available here.
Abstract
The present study was undertaken to clone, express rabies virus glycoprotein (RVG) and to identify potential T-cell epitopes on it. RVG gene (1590 bp) was amplified using gene specific primers. The amplified product was cloned into pTZ57R/T cloning vector by TA cloning. RVG gene was...
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PMID: 21870427
PDF is available here.
PDF is available here.
Abstract
We have demonstrated that by temporarily removing Kupffer cells (KCs), the transgene expression levels mediated by retrograde intrabiliary infusion (RII) of plasmid DNA, polyethylenimine-DNA, and chitosan nanoparticles were enhanced by 1,927-, 131-, and 23,450-fold, respectively, in comparison with...
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PMID: 21091274
PDF is available here.
PDF is available here.
Abstract
We analyzed TRPC6 in 33 Italian children with sporadic early-onset SRNS and three Italian families with adult-onset FSGS. Design, setting, participants, & measurements TRPC6 mutation analysis was performed through PCR and sequencing. The effects of the detected amino acid substitutions were analyzed...
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PMID: 21734084
PDF is available here.
PDF is available here.
Oral Oncology (47)9 2011
Abstract
The HIV/SIV accessory protein Nef is known to down-modulate cell surface receptors that are required for virus entry such as CD4, CCR5 and CXCR4 to block lethal viral superinfection of the infected cell. The chemokine receptor CXCR4 also plays an important role in promoting cell proliferation, metast...
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PMID: 21763177
PDF is available here.
PDF is available here.
Biochimica et Biophysica Acta (1814)7 2011
Abstract
We next characterized specific enzymes in the nNOS-SUMO-1 conjugation cycle at the cellular level. SUMO-1 conjugation of nNOS depended on Ubc9 (E2). The interaction between nNOS and Ubc9 was facilitated by PIASxβ (E3). On the other hand, SUMO-1 was deconjugated from nNOS by SENP1 and SENP2. Overall...
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PMID: 21545853
PDF is available here.
PDF is available here.
Abstract
We have demonstrated that this is associated with up-regulation of the five microRNAs miR-29c, miR-369-5p, miR-371, miR-499, and let-7f. In this study, we examined the role of these senescence-associated microRNAs for cellular aging and differentiation of MSC. Proliferation was reduced upon transfec...
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PMID: 21660946
PDF is available here.
PDF is available here.
Biomacromolecules (12)6 2011
Abstract
We have designed and produced a modular, recombinant resilin-like polypeptide bearing both mechanically active and biologically active domains to create novel biomaterial microenvironments for engineering mechanically active tissues such as blood vessels, cardiovascular tissues, and vocal folds. Pre...
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PMID: 21553895
PDF is available here.
PDF is available here.
Abstract
We show that activation of the nuclear receptor farnesoid X receptor (FXR) induces AKR1B7 expression in the liver and intestine, and reduces the levels of malondialdehyde (MDA), the end product of lipid peroxidation, in the intestine but not in the liver. To determine whether AKR1B7 regulates MDA le...
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PMID: 21642744
PDF is available here.
PDF is available here.
Abstract
We show that the stress-dependent phosphorylation of huntingtin at Ser13 and Ser16 affects N17 conformation and targets full-length huntingtin to chromatin-dependent subregions of the nucleus, the mitotic spindle and cleavage furrow during cell division. Polyglutamine-expanded mutant huntingtin is h...
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PMID: 21623356
PDF is available here.
PDF is available here.
Abstract
We investigate the DNA ligase requirement of chromosomal translocation formation in mouse cells. Mammals have two other DNA ligases, Lig1 and Lig3, in addition to Lig4. As deletion of Lig3 results in cellular lethality due to its requirement in mitochondria, we used recently developed cell lines def...
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PMID: 21655080
PDF is available here.
PDF is available here.
Abstract
We performed a global proteomic profiling to identify the downstream functional proteins of HBx, thereby detecting the mechanisms of action of HBx on virion replication.
HBx in the HepG2.2.15 cell line was knocked down by the transfection of small interfering RNA (siRNA). The replica...
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PMID: 21669574
PDF is available here.
PDF is available here.
Abstract
P-glycoprotein (P-gp) mediates efflux of xenobiotics and bacterial toxins from the intestinal mucosa into the lumen. Dysregulation of P-gp has been implicated in inflammatory bowel disease. Certain probiotics have been shown to be effective in treating inflammatory bowel disease. However, direct effe...
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PMID: 21350189
PDF is available here.
PDF is available here.
Abstract
Hydrogen sulfide (H(2)S) is produced endogenously by L-cysteine metabolism. H(2)S modulates several ion channels with an unclear mechanism of action. A possible mechanism is through reduction-oxidation reactions attributable to the redox potential of the sulfur moiety. The aims of this study were to...
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PMID: 21393430
PDF is available here.
PDF is available here.
Abstract
We hypothesize that dynamic movement of cav-1 is essential for physiological contractile response of colonic smooth muscle....
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PMID: 21372166
PDF is available here.
PDF is available here.
Abstract
We hypothesized that MUC17 has a role in protection of the intestinal mucosa against luminal pathogens. Human intestinal cell lines were transfected by electroporation (Caco-2 and HT 29/19A) and by retroviral expression vector (LS174T, a cell line with high levels of MUC17 expression) using MUC17 si...
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PMID: 21393431
PDF is available here.
PDF is available here.
Abstract
Our results indicated that occludin small-interfering RNA (siRNA) transfection causes an increase in transepithelial flux of various-sized probes, including urea, mannitol, inulin, and dextran, across the Caco-2 monolayers, without affecting the transepithelial resistance. The increase in relative f...
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PMID: 21415414
PDF is available here.
PDF is available here.
Abstract
We examined the role of polyamines in the regulation of the guanine nucleotide exchange factor, diffuse B-cell lymphoma (Dbl), for Rho GTPases. Polyamine depletion decreased the level as well as the activation of Dbl protein. Dbl knockdown by siRNA altered cytoskeletal structure and decreased Rac1 a...
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PMID: 21372162
PDF is available here.
PDF is available here.
Abstract
We investigated the effect of stable over-expression of constitutively active HIF-1α on cardiosphere-derived cell (CDC) engraftment and left ventricular function. CDCs were transduced with a lentivirus expressing a constitutively active mutant of human HIF-1α (LVHIF-1α). Two million male rat CDCs...
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PMID: 21538185
PDF is available here.
PDF is available here.
Molecular Therapy (19)6 2011
Abstract
We investigated its potential as a therapeutic target in NB by generating anti-GD₂-targeted nanoparticles that carry ALK-directed siRNA, which are specifically and efficiently delivered to GD₂-expressing NB cells. Relative to free ALK-siRNA, anti-GD₂-targeted liposomal formulations of ALK-siRN...
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PMID: 21487394
PDF is available here.
PDF is available here.
Protein Journal (30)5 2011
Abstract
We utilized a proteomics strategy to characterize proteome-wide alterations between MP-treated MKN28 lines and their untreated counterparts. A total of 97 spots were positively identified as differentially expressed, and of these 62 proteins were up-regulated, whereas 35 proteins were down-regulated...
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PMID: 21574062
PDF is available here.
PDF is available here.
Abstract
The effects of red ginseng extract on lipid metabolism were examined in ovariectomized rats. Twenty-four female Sprague-Dawley rats (210 +/- 20 g) were studied for 10 weeks. The rats were divided into four groups: (I) "sham" non-ovariectomized rats treated with olive oil, (II) control ovariectomized...
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PMID: 21699086
PDF is available here.
PDF is available here.
Psychoneuroendocrinology (36)5 2011
Abstract
We studied MR gene haplotypes constituted by the two single nucleotide polymorphisms MR-2G/C (rs2070951) and MRI180V (rs5522). The haplotypes showed differences in cortisol-induced gene transcription and protein expression while the structural variant MRI180V did not affect ligand binding. Moreover,...
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PMID: 21095064
PDF is available here.
PDF is available here.
Abstract
Abstract
We analysed how signalling pathways can still process information quantitatively despite strong heterogeneity in protein levels. We systematically perturbed the protein levels of Erk, the terminal kinase in the MAPK signalling pathway in a panel of human cell lines. We found that the steady-state ph...
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PMID: 21613978
PDF is available here.
PDF is available here.
Quantitative analysis of transient and sustained transforming growth factor-beta signaling dynamics.
Abstract
We discovered that cells respond differently to continuous and pulsating TGF-β stimulation. The TGF-β pathway elicits a transient signaling response to a single pulse of TGF-β stimulation, whereas it is capable of integrating repeated pulses of ligand stimulation at short time interval, resulting...
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PMID: 21613981
PDF is available here.
PDF is available here.
Abstract
We find that leucine deprivation causes the caspase-dependent apoptotic death of melanoma cells because it fails to appropriately activate autophagy. Hyperactivation of the RAS-MEK pathway, which is common in melanoma, prevents leucine deprivation from inhibiting mTORC1, the main repressor of autoph...
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PMID: 21575862
PDF is available here.
PDF is available here.
Abstract
We show that a substantial number of serine hydrolases can be irreversibly inactivated by 1,2,3-triazole ureas, which show negligible cross-reactivity with other protein classes. Rapid lead optimization by click chemistry-enabled synthesis and competitive activity-based profiling identified 1,2,3-tr...
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PMID: 21572424
PDF is available here.
PDF is available here.
Cancer Cell (19)5 2011
Abstract
We find that AR transcriptional output is decreased in human and murine tumors with PTEN deletion and that PI3K pathway inhibition activates AR signaling by relieving feedback inhibition of HER kinases. Similarly, AR inhibition activates AKT signaling by reducing levels of the AKT phosphatase PHLPP....
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PMID: 21575859
PDF is available here.
PDF is available here.
Cancer Cell (19)5 2011
Abstract
We investigate the mechanisms by which ETS fusions mediate their effects, and find that the product of the predominant ETS gene fusion, TMPRSS2:ERG, interacts in a DNA-independent manner with the enzyme poly (ADP-ribose) polymerase 1 (PARP1) and the catalytic subunit of DNA protein kinase (DNA-PKcs)...
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PMID: 21575865
PDF is available here.
PDF is available here.
Abstract
We describe here different strategies for overexpressing functional M3R on the perspective of future biophysical studies. To achieve this goal, four tagged M3R genes were engineered and codon optimized. Different heterologous expression systems, including mammalian cells and viral transfection, were...
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PMID: 21548142
PDF is available here.
PDF is available here.
Abstract
The insulin-like growth factor 1 receptor (IGF-1R) is involved in transformation, survival, mitogenesis and differentiation. It is overexpressed in many tumors and a validated target for anticancer therapy. In cell-free systems, polypyrimidic peptide nucleic acids (PNAs) can form triplex-like structu...
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PMID: 21402719
PDF is available here.
PDF is available here.
Abstract
We studied some TDP-43 fragments and their effects on RNA processing in cell models. The results show that the 35-kDa fragment of TDP-43 (namely TDP-35, residues 90-414), but not TDP-25A (184-414), is capable of forming cytoplasmic inclusion bodies and altering pre-mRNA splicing. The inclusions form...
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PMID: 21450909
PDF is available here.
PDF is available here.
Abstract
We investigated whether this polymorphism affects either transcription or translation using luciferase reporter constructs containing variant GCLC 5' UTRs. Higher luciferase activity was observed in HepG2 and human embryonic kidney 293 (HEK293) cells transfected with constructs containing either 8 o...
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PMID: 21444626
PDF is available here.
PDF is available here.
Abstract
We propose a model whereby an N-terminal signal sequence, xLxxKN, directs ABCA transporters to a post-Golgi vesicular sorting station where additional signals may be required for selective delivery of individual transporters to final subcellular destinations....
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PMID: 21586796
PDF is available here.
PDF is available here.
Abstract
We chose bicistronic lentiviral vectors coexpressing fluorescent reporters (eGFP, dTomato, Cerulean, or Venus) along with the canonical RFs to transduce a bulk of murine embryonic fibroblasts (MEFs). Using a flow cytometric approach, we were able to independently and proportionally quantify all fluo...
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PMID: 21548079
PDF is available here.
PDF is available here.
Abstract
We examined the role of mitochondrial dysfunction (MtD) in Aldo-treated podocytes in vitro and in vivo. Exposure of podocytes to Aldo reduced nephrin expression dose dependently, accompanied by increased production of reactive oxygen species (ROS). The ROS generation and podocyte damage were abolish...
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PMID: 21514419
PDF is available here.
PDF is available here.
Xenobiotica (41)5 2011
Abstract
We have optimized the electroporation conditions in a large mammal model, i.e. pig. The parameters optimized include electric field intensity, number of pulses, lag time between plasmid injection and electroporation, and plasmid delivery volume. Constant current pulses, between 0.4 and 0.6 A, applie...
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PMID: 18370201
PDF is available here.
PDF is available here.
Xenobiotica (41)5 2011
Abstract
The discovery of novel target antigens for antibody-based immunotherapy is still a major challenge. Antibody phage display is one of the technologies that is widely applied for the identification of novel cell surface molecules on intact eukaryotic cells and many reports describe the isolation of ph...
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PMID: 19153691
PDF is available here.
PDF is available here.
Xenobiotica (41)5 2011
Abstract
We discuss that the DNA delivery into skin can be greatly enhanced by topical electroporation of the DNA injection site in rabbits using a tweezer electrode. Furthermore, the immune responses following a DNA vaccine delivery by using electroporation have been explored. Electroporation shows great po...
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PMID: 18370202
PDF is available here.
PDF is available here.
Xenobiotica (41)5 2011
Abstract
We provide step-by-step methods on how to utilize synthetic precursor and antisense inhibitor molecules for understanding miRNA function....
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PMID: 18369991
PDF is available here.
PDF is available here.
Abstract
We identified a lumbar spine BMD-associated SNP, rs312009, located in the LRP5 5' region. A RUNX2 binding site was identified in this region by gel-shift experiments. Here we test the functionality of this SNP and examine whether RUNX2 is indeed a regulator of LRP5 expression. Gene reporter assays w...
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PMID: 21542013
PDF is available here.
PDF is available here.
Abstract
To investigate the relationship between the expression of thymidine phosphorylase (TP) and the sensitivity of gastric carcinoma to 5-fluorouracil (5-FU) and its prodrugs.
Gastric carcinoma cell line AGS was transfected with recombinant plasmid pEGFP-N1-TP or control p...
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PMID: 21756828
PDF is available here.
PDF is available here.
Abstract
Abstract
We report a new selectable marker that could allow multiple transfections. We have taken advantage of our finding that a critical function of the mitochondrial electron transport chain (mtETC) in the erythrocytic stages of P. falciparum is the regeneration of ubiquinone as co-substrate of dihydrooro...
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PMID: 21251930
PDF is available here.
PDF is available here.