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Gene Transfer Techniques (3):
Recent article
Gene Transfer Techniques
Journal of Immunotherapy (34)8 2011
Abstract
We examined the possibility of antitumor activity by expressing LIGHT in cervical cancer (CC) model. A recombinant adeno-associated virus (AAV) vector was chosen for the transfer, based on its transfection efficiency and lack of detectable pathology. In vitro transfer of recombinant AAV vector expre...
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PMID: 21904218
PDF is available here.
PDF is available here.
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Abstract
Polyethylenimine-cyclodextrin-tegafur (PEI-CyD-tegafur) conjugate was synthesized as a novel multifunctional prodrug of tegafur for co-delivery of chemotherapeutic agent tegafur and enhanced green fluorescent protein (EGFP) reporter plasmid DNA. Conjugation of tegafur to PEI-CyD via...
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PMID: 21887847
PDF is available here.
PDF is available here.
Abstract
We found drug-inducible store-operated Ca(2+) entry in neonatal rat cardiomyocytes, which was dependent on STIM1. Although this STIM1-dependent, drug-inducible store-operated Ca(2+) entry was only marginal in adult cardiomyocytes isolated from control hearts, it increased significantly in cardiomyoc...
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PMID: 21810664
PDF is available here.
PDF is available here.
Abstract
Angiogenesis represents the formation of new capillaries from existing vasculature, and as such plays a critical role in the response to ischemia in the setting of chronic coronary artery and peripheral vascular disease. Recent technological advances in non-invasive imaging modalitie...
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PMID: 21538181
PDF is available here.
PDF is available here.
Abstract
We have demonstrated that by temporarily removing Kupffer cells (KCs), the transgene expression levels mediated by retrograde intrabiliary infusion (RII) of plasmid DNA, polyethylenimine-DNA, and chitosan nanoparticles were enhanced by 1,927-, 131-, and 23,450-fold, respectively, in comparison with...
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PMID: 21091274
PDF is available here.
PDF is available here.
Abstract
-Local intramyocardial BNP gene delivery improves cardiac function and attenuates adverse post-infarction and angiotensin II-induced remodeling. These results also indicate that myocardial BNP has pleiotropic, context-dependent favourable actions on cardiac function, and suggest that BNP acts locall...
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PMID: 21558448
PDF is available here.
PDF is available here.
Mitochondrion (11)4 2011
Abstract
Mutations in mitochondrial DNA (mtDNA) generate multi-system disorders due to failure of ATP production. A cybrid containing a 1.9-kb mtDNA deletion from a patient with Kearns Sayre Syndrome is respiration-defective and grows glycolytically. When treated with a ribonucleoprotein (RNP...
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PMID: 21406250
PDF is available here.
PDF is available here.
The Lancet (377)9781 2011
Abstract
We tested the hypothesis that non-viral 1 fibroblast growth factor (NV1FGF) would improve amputation-free survival.
In this phase 3 trial (EFC6145/TAMARIS), 525 patients with critical limb ischaemia unsuitable for revascularisation were enrolled from 171 sites in 30 countries. All ha...
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PMID: 21621834
PDF is available here.
PDF is available here.
Abstract
Our laboratory has previously demonstrated that viral administration of glial cell line-derived neurotrophic factor (AdGDNF), one week prior to a controlled cortical impact (CCI) over the forelimb sensorimotor cortex of the rat (FL-SMC) is neuroprotective, but does not significantly enhance recovery...
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PMID: 21672665
PDF is available here.
PDF is available here.
Abstract
This review will cover the current strategies that are being adopted to efficiently deliver small interfering RNA using nonviral vectors, including the use of polymers such as polyethylenimine, poly(lactic-co-glycolic acid), polypeptides, chitosan, cyclodextrin, dendrimers, and polym...
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PMID: 21749290
PDF is available here.
PDF is available here.
Protein Journal (30)5 2011
Abstract
We utilized a proteomics strategy to characterize proteome-wide alterations between MP-treated MKN28 lines and their untreated counterparts. A total of 97 spots were positively identified as differentially expressed, and of these 62 proteins were up-regulated, whereas 35 proteins were down-regulated...
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PMID: 21574062
PDF is available here.
PDF is available here.
Abstract
We describe the superiority of γ-retroviral vectors compared with lentiviral vectors for transduction of murine T cells and surprisingly illustrate robust gene-transfer into phenotypically naive/memory-stem cell like (TN/TSCM; CD62L/CD44) and central memory (TCM; CD62L/CD44) CD8 T cells using murin...
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PMID: 21499127
PDF is available here.
PDF is available here.
Abstract
We show that oligonucleotide-oligospermine conjugates (Zip nucleic acids or ZNAs) can help overcome these shortcomings by serving as effective antisense and antigene agents. Conjugates containing DNA and locked nucleic acid (LNA) oligonucleotides are active, and oligospermine conjugation facilitates...
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PMID: 21539318
PDF is available here.
PDF is available here.
Xenobiotica (41)5 2011
Abstract
We detail a representative example of large-mammal siRNA use....
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PMID: 18369784
PDF is available here.
PDF is available here.
Abstract
Gene therapy holds the promise of correcting a genetic defect. It can be achieved with the introduction of a normal wild-type transgene into specific cells of the patient where the endogenous gene is underexpressing or by the introduction of a therapeutic agent, such as, antisense ol...
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PMID: 21291381
PDF is available here.
PDF is available here.
Abstract
The aim of this study was to compare gene silencing in bovine zygotes when small interfering RNAs (siRNAs) were introduced into bovine zygotes by microinjection or lipid-based transfection. In Experiment 1, E-cadherin siRNA was injected at 100 or 375 µM and compared with PBS-injected and non-injecte...
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PMID: 21557920
PDF is available here.
PDF is available here.
Abstract
We have established an MHC class II molecule-expressing B16F1 murine melanoma cell line (B16F1- CIITA) by transduction of the CIITA (Class II transactivator) gene. Exosomes from B16-CII cells (CIITA- Exo) contained a high amount of MHC class II as well as a tumor antigen TRP2. When loaded on dendrit...
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PMID: 21464590
PDF is available here.
PDF is available here.
Abstract
Advances in gene-based medicine since 1990s have ushered in new therapeutic strategy of gene therapy for inborn error genetic diseases and cancer. Malignant brain tumors such as glioblastoma multiforme and medulloblastoma remain virtually untreatable and lethal. Currently available t...
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PMID: 20521177
PDF is available here.
PDF is available here.
Clinical Cancer Research (17)9 2011
Abstract
Salivary glands are significantly affected when head and neck cancer patients are treated by radiation. We evaluated the effect of human keratinocyte growth factor (hKGF) gene transfer to murine salivary glands on the prevention of radiation-induced salivary hypofunction.
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PMID: 21367751
PDF is available here.
PDF is available here.
Abstract
We investigate what factors are secreted from skeletal muscle cells (SkMCs) transfected with bFGF gene and whether they participate in endothelial cell migration. We constructed replication-defective adenovirus vectors containing the human bFGF gene (Ad/bFGF) or a control LacZ gene (Ad/LacZ) and obt...
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PMID: 21350328
PDF is available here.
PDF is available here.
Thrombosis and Haemostasis (105)4 2011
Abstract
A patient with severe haemophilia B with a glycine-to-valine missense mutation at residue 190 (c25, chymotrypsin numbering) in factor IX (FIX; FIX-G190V or FIX-FuChou) had
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PMID: 21301787
PDF is available here.
PDF is available here.
Abstract
A family of biodegradable poly(amine-co-esters) was synthesized in one step via enzymatic copolymerization of diesters with amino-substituted diols. Diesters of length C(4) -C(12) (i.e., from succinate to dodecanedioate) were successfully copolymerized with diethanolamines with eithe...
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PMID: 21171165
PDF is available here.
PDF is available here.
Xi bao yu fen zi mian yi xue za zhi = Chinese journal of cellular and molecular immunology (27)2 2011
Abstract
Chimeric adenovirus Ad5/11-E1-CMV-endo-K5/E3-CMV-eGFP could successfully express eGFP and endostatin-K5. Chimeric adenoviral vector significantly enhances the infection efficiency for human glioblastoma cell line A172 and breast cancer cell line MDA-MB-231 compared with unmodified adenoviral vector...
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PMID: 21315041
PDF is available here.
PDF is available here.
Abstract
The genetic delivery of therapeutic monoclonal antibodies (mAbs) by in vivo production may offer a new solution to the current problems in the mAb therapy for microbial diseases. Herein, plasmids encoding the neutralizing mAb against hemagglutinin (HA) of A/PR/8/34 influenza virus (IFV) were electro-...
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PMID: 21266754
PDF is available here.
PDF is available here.
Abstract
Our understanding of siRNA and miRNA-based regulation has direct implications for fundamental biology as well as disease aetiology and treatment as it is discussed in this review on 'new techniques in molecular biology'.
2011 S. Karger AG, Basel....
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PMID: 21252559
PDF is available here.
PDF is available here.
Oligonucleotides (21)2 2011
Abstract
We previously reported an angiogenic endothelial cell-targeted polymeric gene carrier, PEI-g-PEG-RGD. This targeted carrier was developed by the conjugation of the ανβ3/ανβ5 integrin-binding RGD peptide (ACDCRGDCFC) to the cationic polymer, branched polyethylenimine, with a hydrophilic polyeth...
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PMID: 21375397
PDF is available here.
PDF is available here.
Abstract
Incorporation of gene into electrospun nanofibers for localized gene transfection of target cells represents a robust platform for tissue regeneration. In this study, a new two-step approach was explored to immobilize DNA onto electrospun nanofibers for effective gene delivery, that...
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PMID: 21105170
PDF is available here.
PDF is available here.
Abstract
We developed a transgenic Lactobacillus acidophilus strain that constitutively expresses IFN-β (La-IFN-β). While pretreatment of mice with control Lactobacillus (La-EV) provided slight protective benefits, La-IFN-β increased sensitivity to DSS. Analysis showed colitic mice pretreated with La-IFN-...
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PMID: 21365015
PDF is available here.
PDF is available here.
Abstract
Among the treatment options that have been developed for cancer, chemotherapy remains 1 of the leading clinical approaches. Chemotherapy can usually control tumor growth at the onset of disease, but its effectiveness becomes limited by the overexpression of transporter proteins respo...
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PMID: 20715163
PDF is available here.
PDF is available here.
Nature Medicine (16)12 2010
Abstract
Behavioural Brain Research (214)2 2010
Abstract
We show that overexpressing ERK2 increases preference for environments previously paired with low doses of cocaine and enhances cocaine-induced locomotion, whereas blocking ERK2 activity blocks cocaine-induced place conditioning and locomotor activity. These results demonstrate that ERK2-signaling w...
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PMID: 20561901
PDF is available here.
PDF is available here.
Brain research (1359) 2010
Abstract
Issues related to the intra-cerebral delivery of glial cell line-derived neurotrophic factor (GDNF) have hampered its progression as a neuroprotective therapy for Parkinson's disease. Ex vivo gene therapy, where cells are virally transduced in vitro to produce a specific protein, may circumvent some...
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PMID: 20732313
PDF is available here.
PDF is available here.
Brain research (1359) 2010
Abstract
We investigated whether ligand-mediated targeting could deliver genes to its secretory epithelium. We show here that when bacteriophages are targeted with epidermal growth factor, they acquire the ability to enter choroid epithelial cells grown in vitro as cell cultures, ex vivo as tissue explants o...
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PMID: 20732308
PDF is available here.
PDF is available here.
Scientific American (303)5 2010
Abstract
Abstract
We developed a recombinant adenoviral vector (AdSiR-MR-1) driving small interfering (si)RNA against MR-1 to evaluate its effect on cardiac hypertrophy in vivo. Cardiac hypertrophy was induced by chronic ANG II infusion in mice; AdSiR-MR-1 was administered via the jugular vein through one bolus injec...
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PMID: 20802139
PDF is available here.
PDF is available here.
Cold Spring Harbor Protocols (2010)11 2010
Abstract
RNA interference (RNAi) is an evolutionarily conserved process that silences gene expression through double-stranded RNA species in a sequence-specific manner. Small interfering RNAs (siRNAs) can promote sequence-specific degradation and/or translational repression of target RNA by a...
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PMID: 21041394
PDF is available here.
PDF is available here.
Nature Methods (7)11 2010
Abstract
We present a flexible and highly specific targeting method for lentiviral vectors based on single-chain antibodies recognizing cell-surface antigens. We generated lentiviral vectors specific for human CD105(+) endothelial cells, human CD133(+) hematopoietic progenitors and mouse GluA-expressing neur...
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PMID: 20935652
PDF is available here.
PDF is available here.
Abstract
We found that specific and sustained GH1 expression did not improve muscle exercise performance despite inducing local muscle hypertrophy. Injection of rAAV2/1-CMV-GH1 had some systemic effects on the liver and heart and on lipid metabolism in the healthy rats. Serum levels of hGH (human growth horm...
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PMID: 20204528
PDF is available here.
PDF is available here.
Abstract
These results demonstrate that the eNOS/NO system provides cardiac protection after MI injury through inhibition of inflammation and suppression of MAPK signaling....
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PMID: 19908164
PDF is available here.
PDF is available here.
Molecular Therapy (18)10 2010
Abstract
We compared the genomic integration efficiencies and transposition site preferences of Sleeping Beauty (SB or SB11), Tol2, and piggyBac (PB) transposon systems in primary T cells derived from peripheral blood lymphocytes (PBL) and umbilical cord blood (UCB). We found that PB demonstrated the highest...
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PMID: 20606646
PDF is available here.
PDF is available here.
Biomacromolecules (11)9 2010
Abstract
These results clearly show that EAPPM is an efficient and safe vector for FGFR-mediated targeted gene delivery both in vitro and in vivo. With low cytotoxicity and high targetability, EAPPM may have great potential as a delivery vector for future cancer gene therapy applications....
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PMID: 20704346
PDF is available here.
PDF is available here.
Abstract
The incorporation of stimuli-responsive properties into nanostructured systems has recently attracted significant attention in the research of intracellular drug/gene delivery. In particular, numerous surface-functionalized, end-capped mesoporous silica nanoparticle (MSN) materials have been designe...
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PMID: 20716017
PDF is available here.
PDF is available here.
Abstract
We compared the rat VEGF120 and rat VEGF164 expression level of the fibroblasts or osteoblasts that were transfected with human VEGF165, with nontransfected control cells. Both the transfected fibroblasts and osteoblasts showed greater expression of rat VEGF164 than nontransfected controls at Day 1...
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PMID: 20736792
PDF is available here.
PDF is available here.
Abstract
We found that at a charge ratio (rho) of 4.0 lipoplexes had optimum characteristics for gene delivery in vitro. To decrease the size of lipoplexes, we used a method of continuous-flow microfluidics. PEGylation of lipoplexes did not hinder internalization, but was found to hamper transfection. To dis...
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PMID: 19947825
PDF is available here.
PDF is available here.
Abstract
We examined the kinetics of endogenous HIF-1alpha during acute and chronic rejection, and the effect of exogenous HIF-1alpha in chronically rejecting rat cardiac allografts.
Heterotopic cardiac transplantations were performed between major MHC-mismatched Dark Agouti and Wistar-Furth rats. Cyclospori...
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PMID: 20580263
PDF is available here.
PDF is available here.
Oncology Reports (24)3 2010
Abstract
We investigated the effect of PEDF gene loaded in PLGA nanoparticles (PEDF-PLGANPs) on the mouse colon carcinoma cells (CT26s) in vitro and in vivo. Blank PLGANPs (bPLGANPs) showed lower cytotoxicity than PEI to the CT26s. In vitro, PEDF-PLGANPs directly induced CT26 apoptosis and inhibit human umbi...
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PMID: 20664971
PDF is available here.
PDF is available here.
Abstract
We developed polylysine based DNA nanoparticles (DNA NPs) that contain disulfide linkage in the carrier and demonstrated that this reducible DNA NP enhances in vitro gene transfer via an extracellular mechanism. Polylysine was conjugated through an N-terminal cysteine to a polyethylene glycol chain...
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PMID: 20438780
PDF is available here.
PDF is available here.
Abstract
Polyplexes consisting of a standard CMV promoter driven luciferase plasmid condensed with PAMAM starburst dendrimers (generation 4 and 5) efficiently transfected tumor cells in vitro. Tail vein injection of PAMAM polyplexes into immune competent mice bearing subcutaneous, well vascularized murine ne...
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PMID: 20438778
PDF is available here.
PDF is available here.
Abstract
The objective of this study was to prepare a series of chitosan-graft-polyethylenimine (chitosan-g-PEI) copolymers as gene carriers with high transfection efficiency and low cytotoxicity. Chitosan-g-PEIs with different molecular weights and segments were successfully synthesized by both oxidation an...
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PMID: 20824956
PDF is available here.
PDF is available here.
Abstract
The proliferation of hPDLCs transduced by adenoviruses coexpressing BMP-7 and IGF-1 was suppressed while their differentiation ability was enhanced. There was a synergism of BMP-7 and IGF-1 in up-regulating alkaline phosphatase activity and mRNA levels of collagen type I and Runx2. Implantation in v...
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PMID: 20412417
PDF is available here.
PDF is available here.
Abstract
The pMEL34 was loaded in elastic cationic niosomes (Tween61/Cholesterol/DDAB at 1:1:0.5 molar ratio) by chloroform film method with sonication and rehydrated with 25% ethanol. The amount of pMEL34 was determined by gel electrophoresis and gel documentation. The maximum loading of pME...
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PMID: 20213835
PDF is available here.
PDF is available here.
Diabetologia (53)8 2010
Abstract
We previously showed the effective delivery of VEGF gene to rat myocardium by an ultrasound-mediated gene-transfer method named ultrasound-targeted microbubble destruction (UTMD). Here we examined the effect of non-viral VEGF delivery using UTMD on transplanted islets in vivo.
A marg...
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PMID: 20405100
PDF is available here.
PDF is available here.
Diabetologia (53)8 2010
Abstract
We previously showed the effective delivery of VEGF gene to rat myocardium by an ultrasound-mediated gene-transfer method named ultrasound-targeted microbubble destruction (UTMD). Here we examined the effect of non-viral VEGF delivery using UTMD on transplanted islets in vivo.
A marg...
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PMID: 20405100
PDF is available here.
PDF is available here.
Diabetologia (53)8 2010
Abstract
We previously showed the effective delivery of VEGF gene to rat myocardium by an ultrasound-mediated gene-transfer method named ultrasound-targeted microbubble destruction (UTMD). Here we examined the effect of non-viral VEGF delivery using UTMD on transplanted islets in vivo.
A marg...
|
PMID: 20405100
PDF is available here.
PDF is available here.
Diabetologia (53)8 2010
Abstract
We previously showed the effective delivery of VEGF gene to rat myocardium by an ultrasound-mediated gene-transfer method named ultrasound-targeted microbubble destruction (UTMD). Here we examined the effect of non-viral VEGF delivery using UTMD on transplanted islets in vivo.
A marg...
|
PMID: 20405100
PDF is available here.
PDF is available here.
Diabetologia (53)8 2010
Abstract
We previously showed the effective delivery of VEGF gene to rat myocardium by an ultrasound-mediated gene-transfer method named ultrasound-targeted microbubble destruction (UTMD). Here we examined the effect of non-viral VEGF delivery using UTMD on transplanted islets in vivo.
A marg...
|
PMID: 20405100
PDF is available here.
PDF is available here.
Diabetologia (53)8 2010
Abstract
We previously showed the effective delivery of VEGF gene to rat myocardium by an ultrasound-mediated gene-transfer method named ultrasound-targeted microbubble destruction (UTMD). Here we examined the effect of non-viral VEGF delivery using UTMD on transplanted islets in vivo.
A marg...
|
PMID: 20405100
PDF is available here.
PDF is available here.
Diabetologia (53)8 2010
Abstract
We previously showed the effective delivery of VEGF gene to rat myocardium by an ultrasound-mediated gene-transfer method named ultrasound-targeted microbubble destruction (UTMD). Here we examined the effect of non-viral VEGF delivery using UTMD on transplanted islets in vivo.
A marg...
|
PMID: 20405100
PDF is available here.
PDF is available here.
Diabetologia (53)8 2010
Abstract
We previously showed the effective delivery of VEGF gene to rat myocardium by an ultrasound-mediated gene-transfer method named ultrasound-targeted microbubble destruction (UTMD). Here we examined the effect of non-viral VEGF delivery using UTMD on transplanted islets in vivo.
A marg...
|
PMID: 20405100
PDF is available here.
PDF is available here.
Diabetologia (53)8 2010
Abstract
We previously showed the effective delivery of VEGF gene to rat myocardium by an ultrasound-mediated gene-transfer method named ultrasound-targeted microbubble destruction (UTMD). Here we examined the effect of non-viral VEGF delivery using UTMD on transplanted islets in vivo.
A marg...
|
PMID: 20405100
PDF is available here.
PDF is available here.