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Primed In Situ Labeling (0):
Recent article
Primed In Situ Labeling
Zhonghua yi xue yi chuan xue za zhi = Zhonghua yixue yichuanxue zazhi = Chinese journal of medical genetics (26)4 2009
Abstract
Within 7 h, the 3 chromosomes were simultaneously marked in the same uncultured amniocyte. The chromosome signals were successfully detected in 69 uncultured samples of amniotic fluid. The results were consistent with that obtained by chromosomes in cultured amniocytes. CONCLUSION: This multicolor p...
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PMID: 20017312
PDF is available here.
PDF is available here.
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Abstract
BAC (bacterial artificial chromosome) clones from the genomic BAC library of the narrow-leafed lupin (Lupinus angustifolius) were used for cytogenetic mapping of mitotic metaphase chromosomes of that species by the BAC-FISH technique. Location of the clones, together with cytogenetic markers localis...
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PMID: 19433903
PDF is available here.
PDF is available here.
Zhonghua yi xue yi chuan xue za zhi = Zhonghua yixue yichuanxue zazhi = Chinese journal of medical genetics (25)6 2008
Abstract
Fifty metaphases were scored. PRINS labeling showed signals for the SRY on the Y chromosome at band Yp11.3 in all metaphases. These signals were as distinct as that from results of FISH. CONCLUSION: This improved method is ideal for rapidly localizing single copy genes and small DNA segments. And PR...
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PMID: 19065537
PDF is available here.
PDF is available here.
Abstract
We described the application of primed in situ (PRINS) labeling approach for the chromosomal mapping of repetitive DNA sequences in Danube salmon (Hucho hucho) (2n = 82, NF = 112). PRINS was successfully performed with primers enabling amplification of 5S rRNA genes (minor rDNAs), NOR building DNA s...
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PMID: 18038183
PDF is available here.
PDF is available here.
Journal of Parasitology (94)4 2008
Abstract
We were able to map these retrotransposons. Perere 03 was localized in the euchromatic regions of the short arm of chromosome 2 and Boudicca in the euchromatic regions of the short arm of chromosomes 2 and Z....
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PMID: 18576699
PDF is available here.
PDF is available here.
Hereditas (Beijing) (30)8 2008
Abstract
Numerical chromosome anomaly was one of the most important kinds of human chromosome diseases by inducing pregnancy loss, miscarriage, infant death, congenital malformations and nerve damage. The present study was to establish a rapid, reliable and reasonable multicolor primed in situ labeling (PRIN...
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PMID: 18779146
PDF is available here.
PDF is available here.
Current protocols in molecular biology / edited by Frederick M. Ausubel ... [et al.] (Chapter 14) 2008
Abstract
This unit provides detailed methods and material descriptions for in situ hybridization following in situ amplification of DNA or RNA by PCR. It includes all essential components of the techniques, including variations suitable for different kinds of tissue and cell preparations. Planning, controls,...
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PMID: 18425762
PDF is available here.
PDF is available here.
Nature Genetics (40)2 2008
Abstract
We report that the defective gene underlying lethal motoneuron syndrome LCCS1 is the mRNA export mediator GLE1. Our finding of mutated GLE1 exposes a common pathway connecting the genes implicated in LCCS1, LCCS2 and LCCS3 and elucidates mRNA processing as a critical molecular mechanism in motoneuro...
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PMID: 18204449
PDF is available here.
PDF is available here.
Abstract
We described application of PRINS technique with telomere human commercial kit to telomere sequences identification. This commercial kit may be use to visualization of interstitial telomeric signal in pig genome. PRINS is attractive complement to FISH for detection of DNA repetitive sequences and di...
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PMID: 18296269
PDF is available here.
PDF is available here.
Abstract
We developed a triple-PRINS reaction on uncultured amniotic cells by omitting the blocking step and taking advantage of mixing two fluorochromes (fluorescein and rhodamine) to create a third color for simultaneous detection in the same amniocytes of three different chromosome targets, e.g., chromoso...
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PMID: 18425471
PDF is available here.
PDF is available here.
Veterinary Journal (174)3 2007
Abstract
A combined primed in situ labelling (PRINS)/4',6-diamino-2-phenylindole (DAPI)/propidium iodide (PI)-fluorescence-banding method was used to characterise telomeres, identify their specific chromosomes and visualise neighbouring heterochromatin in 25 artificial insemination (AI) bulls. A highly heter...
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PMID: 17512764
PDF is available here.
PDF is available here.
Zhonghua yi xue yi chuan xue za zhi = Zhonghua yixue yichuanxue zazhi = Chinese journal of medical genetics (24)5 2007
Abstract
Chromosome 18, X and Y had been simultaneously and specifically marked within 3 hours. The frequency of successful labeling reached 90% both in dual-color and triple-color test. Two chromosome X had been correctly showed in lymphocyte sample of Klinerfelter's syndrome. CONCLUSION: Numerical chromoso...
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PMID: 17922416
PDF is available here.
PDF is available here.
Human Pathology (38)8 2007
Abstract
Molecular in situ-based assays are a useful adjunct to the diagnosis of viral infections by the surgical and cytopathologist. In some cases, the viral nucleic acids and/or proteins are abundant and easily detected by in situ hybridization or immunohistochemistry. In other cases, such as the one inte...
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PMID: 17640551
PDF is available here.
PDF is available here.
Zhonghua yi xue yi chuan xue za zhi = Zhonghua yixue yichuanxue zazhi = Chinese journal of medical genetics (24)4 2007
Abstract
The specific chromosomes were obtained on both metaphase and interphase nuclei. In more than 98% of the samples PRINS reactions with primers X and Y were successfully induced. One sample was properly identified and correctly scored as 47, XXY. CONCLUSION: The results suggest that PRINS technique is...
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PMID: 17680549
PDF is available here.
PDF is available here.
Abstract
An optimized protocol for the spreading of condensed metaphase chromosomes in teflon-coated diagnostic well-slides was developed. Applying this protocol we generated specimens for target primed rolling circle DNA synthesis of padlock probes recognizing a 40 nucleotide sequence in the male specific r...
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PMID: 17997865
PDF is available here.
PDF is available here.
BMC Biotechnology (7)1 2007
Abstract
We present here a proof of principle investigation of a novel rolling circle technology for the detection of non-polyadenylated RNA molecules in situ, including a new probe format (the Turtle Probe) and optimized procedures for its use on formalin fixed paraffin embedded tissue sections and in solid...
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PMID: 17945012
PDF is available here.
PDF is available here.
Cytogenetic and Genome Research (119)3-4 2007
Abstract
We used DNA reassociation kinetics to isolate the highly repeated fraction of the banana genome (M. acuminata 'Calcutta 4'). Two libraries, one prepared from Cot
PDF is available here.
PDF is available here.
Abstract
Two molecular cytogenetics methods, PRINS (primed in situ DNA labeling) and C-PRINS (cycling PRINS), were optimized for the physical mapping of several types of DNA sequences on the mitotic chromosomes of the narrow-leafed lupin (Lupinus angustifolius L.). The fragment of the FokI element from Vicia...
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PMID: 17103086
PDF is available here.
PDF is available here.
Abstract
Various staining patterns were observed in the 20 cases examined, and HPV-positive cells were localized within the surface epithelium as well as in neoplastic cells. We demonstrated that HPV-DNA could be detected in paraffin sections using either the method of in situ PCR or ISH, providing an approp...
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PMID: 17181737
PDF is available here.
PDF is available here.
Journal of Biological Chemistry (281)31 2006
Modifications in Thermus thermophilus 23 S ribosomal RNA are centered in regions of RNA-RNA contact.
Abstract
We investigated the modification status of Thermus thermophilus 5 S and 23 S ribosomal RNA by mass spectrometry and chemical derivatization/primer extension. A total of eleven modified nucleotides was found in 23 S rRNA, of which eight were singly methylated nucleotides and three were pseudouridines...
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PMID: 16731530
PDF is available here.
PDF is available here.
Abstract
Both the primed in situ (PRINS) and the peptide nucleic acid-fluorescence in situ hybridization (PNA-FISH) techniques constitute alternatives to the conventional (fluorescence in situ hybridization, FISH) procedure for chromosomal investigations. The PRINS reaction is based on the use of a DNA polym...
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PMID: 16763713
PDF is available here.
PDF is available here.
Genetika (Moskva) (42)6 2006
Abstract
We used primers, complementary to consensus sequences of two short retroposons: mammalian element MIR and the SOR element from the genome of Sorex araneus. Genetic differentiation among eleven populations of the common shrew from eight chromosome races was estimated. The NP and MJ analyses, as well...
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PMID: 16871777
PDF is available here.
PDF is available here.
Journal of Heredity (97)4 2006
Abstract
In maize, the st1 mutant has been observed to result in chromosomes that stick together during both mitotic and meiotic anaphase. These sticky chromosomes result in abnormal chromosome separation at anaphase. Although the mechanism producing the st1 mutant phenotype is unknown, delayed replication o...
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PMID: 16837564
PDF is available here.
PDF is available here.
Mikrobiologiia (75)6 2006
Abstract
The biogeochemical and molecular biological study of the chemocline and sediments of saline meromictic lakes Shira and Shunet (Khakass Republic, Russia) was performed. A marked increase in the rates of sulfate reduction and methanogenesis was revealed at the medium depths of the chemocline. The rate...
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PMID: 17205809
PDF is available here.
PDF is available here.
Prenatal Diagnosis (26)1 2006
Abstract
Our current results are in agreement with the results previously reported by other groups showing that the number of fetal cells in maternal blood in trisomic 21 pregnancies is higher than in normal pregnancies. This high number of fetal cells is regarded as an advantage for the development of a non...
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PMID: 16374897
PDF is available here.
PDF is available here.
Abstract
An ultrarapid three- and four-color primed in situ (PRINS) procedure has been developed for rapid chromosome identification and aneuploidy assessment on isolated cells. Based on the direct in situ mixing of fluorochromes (fluorescein isothiocyanate, tetramethylrhodamine isothiocyanate, Cascade Blue)...
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PMID: 16861761
PDF is available here.
PDF is available here.
Abstract
We describe an alternative molecular cytogenetic procedure that is the primed in situ labeling (PRINS). This technique consists of annealing oligonucleotides specific to individual chromosome targets and in situ elongation using Taq DNA polymerase to incorporate labeled dUTPs. The sites of the newly...
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PMID: 16861760
PDF is available here.
PDF is available here.
Abstract
Micronuclei are indirect but reliable indicators of chromosome damage, formed at the mitotic division after either chromosome loss or chromosome breakage events, and identified at interphase as small bodies of extra-chromatin in the cytoplasm of mammalian cells. In this chapter, the procedures for s...
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PMID: 16861755
PDF is available here.
PDF is available here.
Abstract
Both primed in situ labeling (PRINS) and peptide nucleic acid (PNA) technologies have emerged as research techniques, but they have quickly evolved to applications in biological diagnosis assays. The two procedures present several features (specificity, discriminating ability, rapidity) that make th...
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PMID: 16861748
PDF is available here.
PDF is available here.
Abstract
We developed a simple and rapid multi-PRINS technique to simultaneously detect three chromosomes in the same cell. With this protocol, one can create a third color using the two most common forms of labeled dUTP (biotin- and digoxigenin-labeled dUTP) and two fluorochromes (fluorescein and rhodamine)...
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PMID: 16861747
PDF is available here.
PDF is available here.
Abstract
We localized the human SRY gene to Yp11.31-p11.32 in chromosome preparations in situ. Locus-specific oligonucleotide probes (i.e., PRINS primers) were annealed to chromosomal DNA fixed on glass slides and extended in the presence of the four trinucleotide precursors, biotin-16-2'-deoxyuridine 5'-tri...
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PMID: 16861750
PDF is available here.
PDF is available here.
Abstract
The introduction of primed in situ labeling (PRINS) into plant cytogenetics provided a novel means for fast and highly specific visualization of DNA sequences on chromosomes and in interphase nuclei. Although the technique does not reach the sensitivity of fluorescence in situ hybridization that is...
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PMID: 16861759
PDF is available here.
PDF is available here.
Abstract
We propose the detailed brain tissue processing protocol that could be used for different molecular cytogenetic approaches to study chromosome complement in interphase nuclei....
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PMID: 16861758
PDF is available here.
PDF is available here.
Abstract
Our group modified the primed in situ labeling (PRINS) method to study RB1 and p53 in cultured bone marrow cells from leukemia patients with known deletions within the 13q14 or 17p13 regions. Locus-specific oligonucleotide probes ("PRINS primers") were annealed to chromosomal DNA on glass slides and...
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PMID: 16861756
PDF is available here.
PDF is available here.
Abstract
Based on the direct in situ mixing of the colors of different fluorochromes (fluorescein isothiocyanate, tetramethylrhodamine isothiocyanate, Cascade Blue) incorporated in sequential primed in situ labeling (PRINS) reactions, a new multicolor PRINS procedure is described, allowing the rapid and dist...
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PMID: 16861752
PDF is available here.
PDF is available here.
Abstract
We developed a simultaneous PRINS and immunofluorescent labeling. We report here the method, which enabled us to visualize the centromere of selected chromosomes and the achromatic spindle on the same picture, a method that may be of a more general application....
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PMID: 16861749
PDF is available here.
PDF is available here.
Abstract
This protocol is a prototype for a series of upcoming procedures aimed at the detection of single molecules of nucleic acids in situ. It uses circular probes that, upon hybridization to their target molecule, can template rolling-circle DNA synthesis, if the target also provides a primer for initiat...
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PMID: 16861751
PDF is available here.
PDF is available here.
Abstract
The protocol is suited for the quantitative and qualitative detection of simple repeat target DNA composed of three or fewer of the four bases A, C, G, and T. A consequence of the lacking base(s) is that such DNA can be synthesized from nucleotide mixtures containing the particular bases as dideoxyn...
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PMID: 16861754
PDF is available here.
PDF is available here.
Abstract
We localized specific deletions of RBM and DAZ, candidate genes for AZF (azoospermia factor) to proximal Yq11.2, the AZF region, in an infertile male. Locus-specific oligonucleotide probes (PRINS primers) were annealed to chromosomal DNA in situ and extended on preparations fixed on glass slides in...
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PMID: 16888350
PDF is available here.
PDF is available here.
Abstract
We describe an approach based on the combined use of primed in situ labeling and fluorescence in situ hybridization techniques for the detection of single DMD exons in fixed metaphase chromosomes and interphase nuclei of both male and female subjects, suggesting the usefulness of this tool in the de...
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PMID: 16861757
PDF is available here.
PDF is available here.
Abstract
The advent of molecular genetic techniques has brought forth new procedures for in situ chromosomal analysis. One of these techniques is the primed in situ labeling (PRINS) procedure, which constitutes a fast and efficient alternative to conventional fluorescence in situ hybridization for nucleic ac...
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PMID: 16861766
PDF is available here.
PDF is available here.
Abstract
These results demonstrate that CPRINS-FISH provides a high sensitivity for microscopic detection of bacteria carrying a specific gene in natural aquatic samples....
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PMID: 16269764
PDF is available here.
PDF is available here.
Clinical Genetics (68)1 2005
Abstract
The aim of this study is to validate the multi-PRINS (primed in situ labelling) technique for simultaneous detection of chromosomes 18, X and Y in uncultured amniocytes for prenatal diagnosis of aneuploidy. The sites of the newly synthesized DNA sequences were showed as fluorescent signals by using...
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PMID: 15952981
PDF is available here.
PDF is available here.
Journal of Heredity (96)5 2005
Abstract
Xenopus laevis is an important reference model organism used in many vertebrate studies. Gene mapping in X. laevis, in comparison to other reference organisms, is in its early stages. Few studies have been conducted to localize DNA sequences on X. laevis chromosomes. Primed in situ labeling (PRINS)...
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PMID: 16135708
PDF is available here.
PDF is available here.
Chromosome Research (13)7 2005
Abstract
We therefore made observations to establish C-banded karyotypes of the agile gibbons included in the lar-group. The data were compared with those of siamangs (the genus Symphalangus), which carry distinctive C-bands, to determine the chromosomal patterns in each group. C-banded chromosomes of agile...
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PMID: 16235121
PDF is available here.
PDF is available here.
Chromosome Research (13)7 2005
Abstract
Chicken repeat 1 (CR1) belongs to the non-long repeat class of retrotransposons. Nearly 100000 repeats interspersed in the chicken genome are subdivided into at least six distinct subfamilies, each 300 bp long and all sharing substantial sequence similarity. CR1-like elements were found in genomes f...
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PMID: 16235116
PDF is available here.
PDF is available here.
Abstract
OBJECTIVES: To evaluate the discriminatory power and usefulness of arbitrarily primed-polymerase chain reaction (AP-PCR) characterization of leptospires with M16 primer. METHODS: AP-PCR fingerprints of 20 reference strains of Leptospira representing 20 different serovars belonging to seven genospeci...
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PMID: 15548317
PDF is available here.
PDF is available here.
Chromosoma (113)4 2004
Abstract
We developed a double-strand PRINS technique, which uses two primers, (TTAGGG)(7) and (CCCTAA)(7), to label both forward and reverse telomeric DNA strands. A total of 120 lymphocyte metaphases obtained from three normal adults were scored to evaluate the labeling efficiency based upon the telomere s...
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PMID: 15349787
PDF is available here.
PDF is available here.
Abstract
A flexible, low-cost alternative to FISH, primed in situ labeling (PRINS) has traditionally been used to detect tandemly repeated target sequences in chromosomes and nuclei. The technique is capable of discriminating among closely related DNA sequences in situ and has the advantage of using very sma...
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PMID: 18428362
PDF is available here.
PDF is available here.
Abstract
To study virus-vector interactions between Soilborne wheat mosaic virus (SBWMV) or Wheat spindle streak mosaic virus (WSSMV) and Polymyxa graminis Ledingham, P. graminis was propagated in plants grown hydroponically. P. graminis accumulated to high levels in several barley cultivars tested. Multiple...
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PMID: 15242168
PDF is available here.
PDF is available here.
Liver International (24)3 2004
Abstract
Without any growth support, normal human liver tissues survived in NOD/SCID mice while maintaining the original viable hepatic architecture. HCV RNA was detected in the mice serum until the fourth week after the inoculation. In situ PCR and immunohistochemistry clearly demonstrated positive signals...
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PMID: 15189278
PDF is available here.
PDF is available here.
médecine/sciences (20)4 2004
Abstract
As a non-isotopic molecular cytogenetic technique, the primed in situ (PRINS) labelling reaction represents a major technological progress achieved in the past decade. It has become a routine technique for the microscopic visualization of specific DNA sequences in cells and nuclei and constitutes a...
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PMID: 15124121
PDF is available here.
PDF is available here.
Journal of Hepatology (40)3 2004
Abstract
The E469E homozygote status for ICAM-1 is associated with protection against PSC....
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PMID: 15123348
PDF is available here.
PDF is available here.
Zhonghua yi xue yi chuan xue za zhi = Zhonghua yixue yichuanxue zazhi = Chinese journal of medical genetics (21)1 2004
Abstract
The specific chromosomes were obtained on both metaphase and interphase nuclei. In more than 95% of the samples, PRINS reactions with primer 18cen were successfully induced. Two samples were properly identified and correctly scored as trisomic 18. CONCLUSION: The results suggest that PRINS technique...
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PMID: 14767916
PDF is available here.
PDF is available here.
Abstract
We focus on the genetic basis of mental retardation (MR), specifically the use of subtelomeric probes to provide new diagnoses in idiopathic MR. We discuss both the background to the clinical demand for diagnoses and the technological advances that culminated in the development of subtelomeric testi...
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PMID: 15603454
PDF is available here.
PDF is available here.
Abstract
Our observations confirm that GC-rich regions are highly mobile genetic structures, and led to ITS formation on brook trout chromosomes.
Copyright 2003 S. Karger AG, Basel...
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PMID: 15218261
PDF is available here.
PDF is available here.
Chromosome Research (12)3 2004
Abstract
We present an improved FISH strategy for differentiating the sex chromosomes of the dioecious model plant, Silene latifolia. Fixed mitotic protoplasts were dropped on a polyethylene naphthalate membrane, the X or Y chromosomes were isolated using nitrogen laser beam microdissection, catapulted by la...
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PMID: 15125638
PDF is available here.
PDF is available here.
Abstract
In 15 cases of NPC tissues, the chromosome 3 had a labeling rate of 88.6% and increasing copy numbers in ten cases (66.7%), and the chromosome 7 had a labeling rate of 87.4% and loss of chromosome in five cases (33.3%). Four cases coexisted with increasing chromosome 3 copy numbers and loss of chrom...
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PMID: 14720386
PDF is available here.
PDF is available here.
Human Molecular Genetics (12)21 2003
Abstract
We have developed a novel fluorescence in-situ hybridization and primed in-situ hybridization (PRINS) approach and rapidly localized the breakpoint of a non-recurrent 22q11.2 translocation, a t(4;22). Multiple primer pairs were designed from the sequence of a 200 kb, chromosome 4, breakpoint-spannin...
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PMID: 12952865
PDF is available here.
PDF is available here.
Chromosoma (112)3 2003
Abstract
We cloned and sequenced the Vicia sativa 25S-18S rDNA intergenic spacer (IGS) and the satellite repeat S12, thought to be related to the spacer sequence. The spacer was shown to contain three types of subrepeats (A, B, and C) with monomers of 173 bp (A), 10 bp (B), and 66 bp (C), separated by unique...
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PMID: 14579131
PDF is available here.
PDF is available here.